Physiological and Molecular Plant Pathology最新文献

{"title":"Ferric ion permease gene VdFtr1 regulates the formation of microsclerotia and pathogenicity in Verticillium dahliae","authors":"Jianfeng Yang ,&nbsp;Yitong Feng ,&nbsp;Xiaoluo Zhang ,&nbsp;Jing Gao ,&nbsp;Qingqin Cao ,&nbsp;Tingting Xiao ,&nbsp;Jun Zhao ,&nbsp;Jian Zhang","doi":"10.1016/j.pmpp.2025.102563","DOIUrl":"10.1016/j.pmpp.2025.102563","url":null,"abstract":"<div><div><em>Verticillium dahliae</em> is a soilborne plant pathogen that infects over 660 plant species, causing <em>Verticillium</em> wilt (VW). Microsclerotia are specialized latent structures generated in the late infection stage of <em>V. dahliae</em>, serving as the primary inoculum for many hosts.</div><div>Our previous research demonstrated that α-1,6-Mannosyltransferase (<em>VdOCH1</em>) regulates microsclerotia formation and pathogenicity in <em>V. dahliae</em>. Subsequently, transcriptome analysis comparing the <em>VdOCH1</em> gene knockout mutant and wild-type (WT) strain of <em>V. dahliae</em> revealed significant alterations in the expression profiles of 1563 genes in the knockout mutant, with 739 genes upregulated and 824 downregulated. The <em>VdFtr1</em> (<em>VDAG_09918</em>) gene was selected for further function analysis based on its significantly down-regulated knockout mutant. The knockout (<em>ΔVdFtr1)</em> and complemented (Com<em>VdFtr1</em>) mutants of the <em>VdFtr1</em> gene were obtained via homologous recombination. The biological characteristics and pathogenicity were compared between WT, <em>ΔVdFtr1,</em> and Com<em>VdFtr1</em> mutants. The results indicate that knockout of <em>VdFtr1</em> (<em>ΔVdFtr1)</em> significantly reduced conidial production and inhibited microsclerotial formation. Concurrently, the expression levels of genes involved in melanin formation and microsclerotium formation were down-regulated, leading to a markedly diminished ability for microsclerotia formation. Also, the pathogenicity of the <em>ΔVdFtr1</em> was dramatically attenuated. All impaired phenotypes of <em>ΔVdFtr1</em> could be restored in Com<em>VdFtr1</em>, in which the full-length <em>VdFtr1</em> gene was complemented in a knockout mutant. In conclusion, <em>VdFtr1</em>, encoded iron transporter permease, functions as a downstream target in α-1,6-Mannosyltransferase (<em>OCH1</em>) regulated microsclerotia formation and pathogenicity signal pathway.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102563"},"PeriodicalIF":2.8,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical review on characterization, management, and challenges of fusarium head blight disease in wheat","authors":"Ali Al-Hashimi ,&nbsp;Omolola Aina ,&nbsp;Augustine Innalegwu Daniel ,&nbsp;Morné Du Plessis ,&nbsp;Marshall Keyster ,&nbsp;Ashwil Klein","doi":"10.1016/j.pmpp.2024.102557","DOIUrl":"10.1016/j.pmpp.2024.102557","url":null,"abstract":"<div><div>Fusarium head blight (FHB) of wheat poses a significant concern globally due to its recurrent epidemics and substantial economic impact. This disease stems from the <em>Fusarium</em> species complex, prominently <em>Fusarium graminearum, F. culmorum,</em> and <em>F. avenaceum</em>, often coexisting with less pathogenic species such as <em>F. sporotrichioides</em>, <em>F. langsethiae, F. triticum, F. poae, Microdochium majus,</em> and <em>Microdochium nivale</em>. FHB's symptoms, such as spike bleaching, mycelium buildup, and shrunken kernels, impact grain quality, and yield, causing annual economic losses exceeding a billion dollars. Furthermore, mycotoxins generated by the FHB complex pose severe health risks to humans, animals, and the ecosystem. Regulatory standards have been enforced to curtail mycotoxin levels in wheat and its derivatives, underscoring the gravity of this issue. This review covers FHB's pathogenesis, its symptoms, identification, and characterisation of <em>Fusarium</em> species within the complex via morphological and molecular techniques. It summarizes the lifecycles of affiliated <em>Fusarium</em> species and their mode of infection. Mycotoxin production, health impacts, and regulatory thresholds in wheat are presented with an overview of management strategies and associated challenges. This review aims to enhance our understanding of FHB intricacies and its management in wheat, fostering more effective disease control measures and reinforcing global food security.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102557"},"PeriodicalIF":2.8,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance, characterization, and pathogenicity of Diplodia and Dothiorella species associated with apple canker and dieback in northern Patagonia, Argentina","authors":"Ximena Virginia Lódolo ,&nbsp;Julia Ousset ,&nbsp;Pedro Mondino-Hintz ,&nbsp;María Cecilia Lutz ,&nbsp;María Cristina Sosa","doi":"10.1016/j.pmpp.2024.102561","DOIUrl":"10.1016/j.pmpp.2024.102561","url":null,"abstract":"<div><div>Canker and dieback diseases affect apple production in northern Patagonia. This study aimed to estimate disease incidence and severity, identify the species of Botryosphaeriaceae associated with the outbreaks, and study their pathogenicity. Rapid increase of infection incidence and severity affecting branches and trunks, but not fruit, was registered after rains. Based on cultural and morphological characteristics, fungal isolates obtained from apple trees were identified as genus <em>Diplodia</em>, with two species and genus <em>Dothiorella</em>. Phylogenetic analysis based on sequencing of ITS, TEF-1α, and TUB-2 gene regions were used to identify <em>Diplodia seriata</em> as the main species, followed by <em>Diplodia mutila</em> and <em>Dothiorella omnivora</em>. <em>Diplodia seriata</em> were grouped by greater virulence in fruit, while <em>D. seriata</em> and <em>D. mutila</em> exhibited greater virulence in shoots. The lowest optimum growth temperature was observed for <em>Do. omnivora.</em> Optimization of orchard management practices is suggested to mitigate risks associated with Botryosphaeriaceae infections.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102561"},"PeriodicalIF":2.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bhendi yellow vein mosaic virus C4 circumvents the RNA silencing pathway by interacting with SAM synthetase and cystathionine beta synthase","authors":"Jeyalakshmi Karanthamalai ,&nbsp;Nagesh Srikakulam ,&nbsp;Kandhalu Sagadevan Dinesh Babu ,&nbsp;Asha Anand ,&nbsp;Sachin Mohite ,&nbsp;Shantha Kumar ,&nbsp;Gopal Pandi","doi":"10.1016/j.pmpp.2024.102549","DOIUrl":"10.1016/j.pmpp.2024.102549","url":null,"abstract":"<div><div>Post-transcriptional and transcriptional gene silencing represents critical innate antiviral defense mechanisms in plants. Viruses encode clusters of viral silencing suppressors to infect plants to counteract the plant's antiviral response effectively. Previous studies have identified Bhendi yellow vein mosaic virus (BYVMV) C4 and C2 proteins as post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS) suppressors, yet the underlying mechanisms of action remain elusive. In our current research, we reveal that the BYVMV C4 protein interacts with S-adenosyl methionine synthetase (SAMS) and cystathionine β-synthase (CBS), both of which play crucial roles in the methyl cycle, either directly or indirectly, thus inhibiting the S-adenosylmethionine (SAM) cycle. Silencing of CBS and SAMS through virus-induced gene silencing (VIGS) resulted in increased symptom susceptibility, elevated viral DNA accumulation, and decreased cytosine methylation on the BYVMV promoter. These findings indicate that BYVMV C4 suppresses PTGS and TGS by inhibiting SAMS and CBS activity, thereby enhancing plant viral infection.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102549"},"PeriodicalIF":2.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of defense-related genes expression in shallot after UV-B-induced resistance against Fusarium acutatum showed time difference","authors":"Belinda Winona ,&nbsp;Widhi Dyah Sawitri ,&nbsp;Muhammad Idris ,&nbsp;Valentina D.S. Handayani ,&nbsp;Arif Wibowo ,&nbsp;Deden D. Matra ,&nbsp;Febri Doni ,&nbsp;Ani Widiastuti","doi":"10.1016/j.pmpp.2024.102559","DOIUrl":"10.1016/j.pmpp.2024.102559","url":null,"abstract":"<div><div>The impact of ultraviolet-B (UV-B) radiation on the activation of key defense-related genes in shallot (<em>Allium cepa</em> L. Aggregatum) is necessary to be investigated. The key resistance genes, i.e. Lipoxygenase 2 (LOX-2), Pathogenesis-related (PR), Peroxidase (PERX), Chitinase (CHIT), and Glucanase (GLU), were monitored at different time and linking them to plant defense pathways like salicylic acid (SA) and jasmonic acid (JA) pathway and UV-B perception responses. We compared the response of UV-B exposure under intensity of 0,25 W/m² for 30 and 150 min. Our results showed increasing the expression levels of LOX-2, PR, PERX, CHIT and GLU in 150 min, while the incidence and intensity of <em>F. acutatum</em> infection was also suppressed when the duration was applied 3 times at night. The UV-B induced resistance is shown to prioritize JA production on the fifth day than SA, however SA accumulation also increased after the treatment. This study provides a novel insight that UV-B induced resistance against <em>F. acutatum</em> infection in shallot by activating key-defense genes and signal transduction pathways in different time which shows a dynamic defense mechanism. These findings underscore the potential of UV-B treatments as an eco-friendly approach to enhance crop resilience and reduce dependence on chemical pesticides in sustainable agriculture.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102559"},"PeriodicalIF":2.8,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing drought-induced immune priming for intergenerational resistance to spot blotch in wheat","authors":"Bandana Devi ,&nbsp;Nidhi Yadav ,&nbsp;Menka Tiwari,&nbsp;Shweta Gupta,&nbsp;Bhumika Chhodvadiya,&nbsp;Prashant Singh","doi":"10.1016/j.pmpp.2024.102558","DOIUrl":"10.1016/j.pmpp.2024.102558","url":null,"abstract":"<div><div>Drought priming has proven to be an effective strategy in modern agriculture for enhancing crop resistance to pathogens. However, the biochemical and metabolic mechanisms underlying these protective effects under biotic stress remain insufficiently understood, offering a valuable opportunity for further research. This study demonstrates that drought-induced priming in wheat (<em>Triticum aestivum</em>) not only improves resistance to <em>Bipolaris sorokiniana</em> (spot blotch) but also transfers this protection to the subsequent generation (G1). Using growth metrics, disease assessments, biochemical analysis, and yield evaluations, we found that progeny of drought-primed plants showed enhanced defense responses under pathogen stress, despite no morphological differences in stress-free conditions. Significant increases in photosynthetic pigments-chlorophyll a (3.80-fold), chlorophyll <em>b</em> (3.27-fold), and carotenoids (2.69-fold) were observed, along with key stress markers like proline (1.78-fold), total phenolics (1.30-fold), and ascorbic acid (2.30-fold). The progeny also exhibited an 8.37-fold reduction in malondialdehyde (MDA) levels, indicating reduced oxidative damage. Under biotic stress, drought-primed progeny displayed increased activities of antioxidant enzymes, including superoxide dismutase (2.35-fold), catalase (2.48-fold), and ascorbate peroxidase (3.37-fold), along with upregulated defense enzymes like peroxidase (1.44-fold), phenylalanine ammonia-lyase (1.97-fold) and chitinase (1.88-fold). These biochemical adjustments optimized resource allocation for both growth and defense. Additionally, drought-primed progeny showed improved yield performance compared to non-primed progeny under stress.</div><div>Our findings highlight drought-induced intergenerational priming as a sustainable, chemical-free strategy for long-term crop protection, offering a path toward resilient crop varieties in the face of global climate challenges.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102558"},"PeriodicalIF":2.8,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing plant-origin bioactive compounds as nematicides –present insights and future directions","authors":"Narmadha Kamaraj ,&nbsp;Seenivasan Nagachandrabose ,&nbsp;Sathyamoorthy Pon ,&nbsp;Devrajan Kandasamy ,&nbsp;Senthil Kuppusamy ,&nbsp;Raja Kalimuthu ,&nbsp;Janani Mani ,&nbsp;Vellaikumar Sampathrajan ,&nbsp;Jayakanthan Mannu","doi":"10.1016/j.pmpp.2024.102543","DOIUrl":"10.1016/j.pmpp.2024.102543","url":null,"abstract":"<div><div>Plant parasitic nematodes (PPNs) are a major challenge to agricultural productivity, causing global crop losses. Although chemical nematicides are still widely used due to their cost-effectiveness and immediate availability, concerns over their environmental and health risks have driven the search for eco-friendly alternatives. Plants with nematicidal properties have emerged as a sustainable option, leading to the development of botanical nematicides. However, large-scale production is constrained by the limited availability of plant material and extracts, prompting efforts to identify plant-origin bioactive compounds (POBCs) with nematicidal potential. Plant-origin bioactive compounds offer several advantages, including reduced toxicity to non-target organisms, environmental safety, and potential for integration into sustainable pest management practices. Over the past two decades, around 114 POBCs from 20 plant families have been identified, including flavonoids, isothiocyanates, saponins, coumarins, terpenes, alkaloids, ketones, glucosinolates, phenolic compounds, phthalides, limonoids, and glycosides. These compounds exhibit nematicidal, nematostatic, membrane-disruptive, egg hatch inhibitory, and developmental inhibitory effects against key PPN species such as <em>Meloidogyne</em> spp., <em>Pratylenchus</em> spp., and <em>Heterodera</em> spp. Effective doses for nematode control range from 11.7 to 188.3 mg/L, 3.2–500 μg/mL, 0.125–1.0 %, and 25–50 ppm. Commercial POBCs like azadirachtin, thymol, and geraniol are marketed globally, though challenges in raw material availability, extraction, and standardization remain. Bioinformatic tools and advanced formulation technologies, such as nanoemulsions and solid lipid nanoparticles, are being explored to enhance the efficacy and stability of POBC-based nematicides. This review highlights the progress in POBC research, their mechanisms of action, and emerging strategies to overcome production and formulation challenges for sustainable nematode management.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102543"},"PeriodicalIF":2.8,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the causal agent of floral malformation as Fusarium complex using metagenomic and metabolomic approaches","authors":"M. Muthukumar,&nbsp;Anju Bajpai,&nbsp;Sumit K. Soni,&nbsp;Sanjay Kumar Singh,&nbsp;Yashi Bajpai,&nbsp;Sandeep Kumar,&nbsp;Laxmi","doi":"10.1016/j.pmpp.2024.102556","DOIUrl":"10.1016/j.pmpp.2024.102556","url":null,"abstract":"<div><div>Mango malformation disease (MMD) is one of the most important diseases that have defied proper understanding and management strategy. The disease is characterized by malformation of vegetative shoots and inflorescences, causing 50–60 % economic losses to nursery and young bearing trees. This study focuses on floral malformation (FM), as it is the most serious concern affecting the flowering and fruiting. The causal agents of floral malformation have been highly debated and Koch postulates could only be partially proven with <em>Fusarium mangiferae</em>. The current modern tools approaches such as metagenomic and metabolomic methods provide robust data for associated pathogen diversity and their metabolites within the malformed mango panicle (MP). Whole metagenome analysis of malformed panicles (cv. Beauty Maclean) showing characteristic symptoms were compared with healthy normal panicle described as control (C), that revealed maximum OTUs representing <em>Fusarium mangiferae,</em> followed by <em>F. proliferatum</em>, <em>F. verticilloides</em>, <em>F. oxysporum</em> and <em>Giberrella fujikorrii</em>. Functional analysis predicted Polyketide synthase gene (<em>FUM1a</em>) involved in Fumonisin/Fusaric acid biosynthesis. Pathway analysis showed unique key regulators of fungal pathogenicity viz., glucosylceramidase, chitin deacetylase etc. in the malformed tissue. Upregulated urea carboxylase and mercuric reductase in the MP sample only raises interesting questions on chemical impact on predisposition of MMD. Zn-peptidases, dipeptidases and cyanide permease were also identified in MP which is involved in fungal virulence and cyanide toxicity induced by <em>Fusarium</em> complex. These results confirm <em>Fusarium</em> complex (<em>Fusarium mangiferae, F. proliferatum</em>, <em>F. verticilloides</em>) as incitants of FM. This was corroborated by standard semi-quantitative PCR and quantitative real time PCR (q-RT-PCR) assay using <em>FUM1a</em> gene in MP and C in three mango cultivars. Differential metabolite analysis through gas chromatography-mass spectrometry (GC-MS) based volatile organic compounds (VOC) detected upregulation of metabolites such as esters and methyl esters of fatty acids that implicate induction of jasmonic acid and fatty acid pathways, fumonisin production and suppression of resistance metabolites in the malformed samples. This study gives clues to understand the genetic and molecular events related to the disease etiology besides confirming the incitant of MMD as <em>Fusarium</em> complex.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102556"},"PeriodicalIF":2.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and biochemical disruptions in Phaseolus vulgaris L. induced by Pseudomonas syringae pv. phaseolicola infection","authors":"Kamran Rahnama ,&nbsp;Robert Whitbread ,&nbsp;Nima Akbari Oghaz ,&nbsp;John F. Farrar","doi":"10.1016/j.pmpp.2024.102553","DOIUrl":"10.1016/j.pmpp.2024.102553","url":null,"abstract":"<div><div>This study examines the physiological and biochemical responses of <em>Phaseolus vulgaris</em> L. cv. Prince to infection by <em>Pseudomonas syringae</em> pv. <em>phaseolicola</em> Race 1 under controlled environmental conditions. Plants were inoculated with a bacterial suspension of 6 × 10⁸ cfu cm⁻³ applied to unifoliate leaves, and disease progression was assessed via microscopic examination and photography. Photosynthetic performance, chlorophyll and carotenoid concentrations, protein content, carbon assimilation, and carbohydrate metabolism were analyzed to elucidate the pathogen's impact on plant physiology. Results revealed the onset of infection symptoms, including necrosis and chlorosis, within 36 h post-inoculation, with a progressive decline in plant health, particularly in unifoliate and trifoliate leaves. Photosynthetic efficiency, measured through oxygen evolution, was significantly impaired in infected tissues, alongside a marked increase in dark respiration rates. Chlorophyll <em>a</em> and <em>b</em>, as well as carotenoid concentrations, were significantly reduced in inoculated areas. Soluble protein levels and carbon translocation, traced via ¹⁴CO₂ labeling, were also adversely affected, with inoculated plants displaying altered carbon partitioning and decreased total soluble carbohydrates, particularly near infection sites. Inoculation reduced relative growth rates and dry mass accumulation, most notably in plants with inoculated unifoliate leaves. These findings provide comprehensive insights into the deleterious effects of bacterial infection on both the structural and functional aspects of plant physiology, demonstrating significant impairments in photosynthetic capacity, protein content, and carbohydrate metabolism. This study offers a deeper understanding of the complex plant-pathogen interactions, highlighting the multifaceted impacts of <em>P. syringae</em> on plant growth and health.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102553"},"PeriodicalIF":2.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput sequencing identification and multiplex RT-PCR detection of pepper viruses","authors":"Chun Luo ,&nbsp;Yao Luo ,&nbsp;Hao Luo ,&nbsp;Ke Han ,&nbsp;Lei Lei ,&nbsp;Meng-ao Jia ,&nbsp;Tengzhi Xu","doi":"10.1016/j.pmpp.2024.102555","DOIUrl":"10.1016/j.pmpp.2024.102555","url":null,"abstract":"<div><div>Peppers are susceptible to a variety of virus infections. Early and accurate detection of virus species is the prerequisite for the virus disease prevention and management to reduce yield and quality losses. This study utilized small RNA sequencing to investigate the viruses in Guizhou peppers. Through extensive bioinformatics analysis, we identified ten virus species infecting Guizhou peppers and obtained the sequences of the conserved protein encoded by these viruses. Our phylogenetic analyses clarified the taxonomic status of the obtained viral isolate. A rapid and cost-effective multiplex PCR method for simultaneous virus detection was developed. By testing the novel developed method using field samples, we found that CMV, PCV1 and BBWV2 were the three most common viruses. This research offers a comprehensive list of viruses affecting peppers in Guizhou and presents an efficient detection method, facilitating our understanding and practical management of pepper virus infections.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"136 ","pages":"Article 102555"},"PeriodicalIF":2.8,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143162768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}