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Nutritional and Bioactive Compounds, Antioxidant Properties and Antibacterial Activity of Chinese Chive (Allium odorum L.). 韭菜(Allium odorum L.)的营养成分、生物活性成分、抗氧化性能和抗菌活性。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-09 DOI: 10.1007/s11130-024-01259-7
Phuritshabam Vivekanandini Devi, Rajinder Kumar Dhall, Jaswinder Kaur Brar

The present study was conducted to generate the comprehensive data on the nutritional composition, minerals, heavy metal, bioactive compounds, antioxidant and antibacterial properties of Chinese chive (Allium odorum L.) grown at two different locations including Manipur and Punjab. The crop grown in Manipur exhibited higher values of nutritional and phytochemical compound values than those grown in Punjab. It exhibited significantly higher amounts of crude protein, crude fiber, potassium, calcium and iron content in the Manipur environment. The allicin content in leaves of Chinese chive on a dry weight (dw) varied from 1.31 to 1.4% and there was non-significant difference in allicin content at both locations. Besides, the heavy metal content of Chinese chive was much below the permissible limits of the World Health Organization. The IC50 value of Chinese chive was 78.908-95.140 µg/ml which imparts strong antioxidant activity and higher DPPH (2,2-diphenyl-1-picrylhydrazyl)radical scavenging activity higher in fresh leaves than dried leaves. Total phenol and total flavonoid were found to be significantly high. In addition, high antibacterial activities were observed against toxin producing pathogenic bacterial strains of Staphylococcus aureus and Enterococcus faecalis. The nutritional and bioactive compounds, antioxidant properties and antibacterial activity of Chinese chive make it ideal for its use as a functional food and/or supplement.

本研究旨在获得在曼尼普尔和旁遮普两个不同地区种植的韭菜(Allium odorum L.)的营养成分、矿物质、重金属、生物活性化合物、抗氧化和抗菌特性的综合数据。曼尼普尔种植的韭菜营养价值和植物化学化合物价值高于旁遮普种植的韭菜。曼尼普尔的粗蛋白、粗纤维、钾、钙和铁含量明显高于旁遮普。韭菜叶片中的大蒜素含量(干重)从 1.31% 到 1.4% 不等,两地的大蒜素含量差异不大。此外,韮菜的重金屬含量遠低於世界衞生組織的允許限值。韭菜的 IC50 值为 78.908-95.140 µg/ml,具有很强的抗氧化活性,鲜叶的 DPPH(2,2-二苯基-1-苦基肼)自由基清除活性高于干叶。总酚和总黄酮的含量也很高。此外,对产生毒素的金黄色葡萄球菌和粪肠球菌等致病菌株也有较高的抗菌活性。韭菜的营养和生物活性化合物、抗氧化特性和抗菌活性使其成为一种理想的功能性食品和/或补充剂。
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引用次数: 0
Mesquite Tree (Prosopis spp.): A Native Resource for the Potential for Human Consumption and Healthcare. 豆科树:一种具有人类消费和保健潜力的本地资源。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-09 DOI: 10.1007/s11130-024-01255-x
Rocio Yaneli Aguirre-Loredo

Mesquite is a tree widely distributed in arid and semi-arid areas of the American continent. Its use dates back to the native cultures of the continent. Its use mainly focuses on obtaining products for human consumption, traditional medicine, fuel, and construction. There are 44 known species, which are widely distributed in the north and south of the American continent, and some of them can be found in Africa, Asia, and Australia. The entire mesquite tree can be used without wasting any part of the plant. Its uses include fodder for small farm animals, furniture manufacturing, firewood for fuel, charcoal for cooking, or biofuel biomass. As for its use in human food, the mature seeds and pods are used to obtain a gluten-free flour added as a supplement in baking corn and wheat products. This flour is rich in fiber, protein, and minerals and low in fat. Due to the presence of compounds such as flavonoids and phenolic compounds in various tissues or sections of the plant, it has a high antioxidant, antibacterial, and antifungal capacity, among other properties that make the Prosopis tree an excellent source of active compounds, flours, natural sugars, and gums that can be used by the food industry to enrich the population's diet. It is an underutilized and widely distributed natural resource in the American continent that should not be ignored.

Mesquite 是一种广泛分布于美洲大陆干旱和半干旱地区的树木。它的使用可以追溯到美洲大陆的本土文化。其用途主要集中在获取供人类消费、传统医药、燃料和建筑用的产品。已知有 44 个物种,广泛分布在美洲大陆的北部和南部,其中一些物种在非洲、亚洲和澳大利亚也能找到。整棵弥猴桃树都可以利用,不会浪费植物的任何部分。其用途包括小型农场动物的饲料、家具制造、燃料木柴、烹饪木炭或生物燃料生物质。至于它在人类食品中的用途,成熟的种子和豆荚可用于制作无麸质面粉,作为烘焙玉米和小麦产品的补充。这种面粉富含纤维、蛋白质和矿物质,脂肪含量低。由于植物的不同组织或部分含有黄酮类和酚类化合物等化合物,它具有很强的抗氧化、抗菌和抗真菌能力,这些特性使芒果树成为活性化合物、面粉、天然糖和胶质的极佳来源,可被食品工业用来丰富人们的饮食。它是美洲大陆一种未得到充分利用且分布广泛的自然资源,不应被忽视。
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引用次数: 0
Phenolic Compounds, Antioxidant Activity, Sensorial Analysis and Metabolic Syndrome Enzyme Inhibition Properties of Calafate (Berberis microphylla) Fruit Juice Fermented by Patagonian Lactic Acid Bacteria. 巴塔哥尼亚乳酸菌发酵卡拉酸(小叶小檗)果汁的酚类化合物、抗氧化活性、感官分析和代谢综合征酶抑制特性
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-07 DOI: 10.1007/s11130-024-01246-y
Brenda Sede Lucena, Julieta Amalia Burini, Omar Federico Ordoñez, Laura Crespo, María Clara Bruzone, Fernanda Mozzi, Micaela Pescuma

Calafate is a native barberry of Patagonia, used in culinary and medicinal practices since ancient times. The aim of this work was to analyze the ability of lactic acid bacteria (LAB) isolated from calafate fruits and flowers, to increase the phenolic compound concentration and antioxidant capacity as well as to inhibit metabolic-related enzymes in fermented calafate juices. The sensory attributes of the selected fermented juice were also analyzed. The LAB strains grew between 1.33 ± 0.03 and 2.61 ± 0.30 log CFU/ml in the calafate juices at 24 h. Fructobacillus fructosus B7 consumed glucose and fructose the most (2.30 ± 0.45 g/L and 3.73 ± 0.44 g/L, respectively) and produced mannitol (3.89 ± 0.77 g/L). The fermented juice by Lacticaseibacillus paracasei B4 showed the highest total phenolic compound concentration (2662.58 ± 344.51 mg GAE/100 ml) and antioxidant capacity (38916.42 ± 2157.52 µmol TE/100 ml). The fermented juices inhibited the activity of metabolic syndrome-related enzymes. The lower IC50 for α-glucosidase activity was observed for F. fructosus B7 and L. paracasei B4 (0.56 ± 0.10 and 0.64 ± 0.05 mg GAE/ml, respectively) fermented juices, while for α-amylase the lowest IC50 values were determined for the L. curvatus B34 and L. paracasei B4 (0.34 ± 0.01 and 0.37 ± 0.06 mg GAE/ml, respectively) juices. The relative amount of isorhamnetins, which can induce GLUT4 translocation to the plasma membrane preventing hyperglycemia, was increased in the L. paracasei B4 fermented juice. The L. paracasei B4 fermented juice had acceptable sensorial values for consumption.

卡拉法特是巴塔哥尼亚的一种本土浆果,自古以来就被用于烹饪和医疗实践。本研究的目的是分析从酸果和花中分离的乳酸菌(LAB)提高酸果发酵汁中酚类化合物浓度、抗氧化能力和抑制代谢相关酶的能力。并对所选发酵汁的感官特性进行了分析。24 h时,乳酸菌生长在1.33±0.03 ~ 2.61±0.30 log CFU/ml之间,果糖芽孢杆菌B7消耗葡萄糖和果糖最多(分别为2.30±0.45 g/L和3.73±0.44 g/L),产生甘露醇(3.89±0.77 g/L)。副干酪乳杆菌B4发酵汁的总酚类化合物浓度最高(2662.58±344.51 mg GAE/100 ml),抗氧化能力最高(38916.42±2157.52µmol TE/100 ml)。发酵果汁抑制代谢综合征相关酶的活性。果糖乳杆菌B7和副干酪乳杆菌B4的α-葡萄糖苷酶活性IC50较低(分别为0.56±0.10和0.64±0.05 mg GAE/ml),而α-淀粉酶活性IC50最低的是曲曲菌B34和副干酪乳杆菌B4(分别为0.34±0.01和0.37±0.06 mg GAE/ml)。异鼠李素的相对含量在副干酪乳杆菌B4发酵汁中有所增加,异鼠李素可诱导GLUT4向质膜转移,防止高血糖。副干酪乳杆菌B4发酵汁具有可接受的感官值。
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引用次数: 0
Alcoholic Extracts from the Ganoderma Lucidum Fermentation Product Alleviated Ethanol-Induced Liver Injury, Gut Leakiness, and Gut Dysbiosis in Mice. 灵芝发酵产物酒精提取物可减轻小鼠乙醇性肝损伤、肠道渗漏和肠道生态失调。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-05 DOI: 10.1007/s11130-024-01271-x
Zhikun Zhao, Xiaoxiao Ma, Mingyan Li, Guangyuan Chen, Libo Qi, Shuang Song, Zhenhao Li, Chunhong Yan

The hepatoprotective effect of the alcoholic extracts of Ganoderma lucidum fermentation products (GFE) was investigated. C57BL/6 mice were pretreated with GFE for 7 days and then subjected to the chronic-binge ethanol feeding model. GFE pretreatment significantly reduced the ethanol-induced elevated serum levels of aspartate aminotransferase (AST) and alanine transaminase (ALT), hepatic steatosis, and increased triglyceride content. GFE pretreatment also altered hepatic alcohol metabolism, suppressed oxidative stress by decreasing the expression of Cyp2e1, and increasing the level of GSH. Lipidmoic analysis revealed that GFE pretreatment effectively increased ratio of phosphatidylcholines /phosphatidylethanolamine (PC/PE) in the liver. Furthermore, mice pretreated with GFE demonstrated decreased hepatic inflammation and plasma lipopolysaccharide (LPS) levels. Additionally, the mRNA expression of gut tight junction proteins such as ZO-1, Occludin and Claudin-1, along with antimicrobial peptide (e.g., Reg3β and Reg3γ) were up-regulated by GFE pretreatment. 16s rRNA sequencing revealed that GFE increased Bacteroidales, Parabacteroides, and Dubosiella, which were associated with hepatic steatosis, inflammation and intestinal barrier function parameters. These results demonstrate that GFE can prevent ethanol-induced liver injury and inflammation, gut leakiness and restore gut microbiota dysbiosis.

研究了灵芝发酵产物醇提物的保肝作用。C57BL/6小鼠经GFE预处理7 d后建立慢性酒精暴食模型。GFE预处理显著降低了乙醇诱导的血清谷草转氨酶(AST)和谷丙转氨酶(ALT)水平升高、肝脂肪变性和甘油三酯含量升高。GFE预处理还通过降低Cyp2e1的表达和提高GSH水平来改变肝脏酒精代谢,抑制氧化应激。脂质学分析显示,GFE预处理能有效提高肝脏中磷脂酰胆碱/磷脂酰乙醇胺(PC/PE)的比值。此外,用GFE预处理的小鼠显示肝脏炎症和血浆脂多糖(LPS)水平降低。此外,GFE预处理还上调了肠道紧密连接蛋白(ZO-1、Occludin和Claudin-1)和抗菌肽(Reg3β和Reg3γ)的mRNA表达。16s rRNA测序结果显示,GFE增加了与肝脂肪变性、炎症和肠屏障功能参数相关的拟杆菌属、拟副杆菌属和杜波菌属。上述结果表明,GFE具有预防乙醇性肝损伤和炎症、肠道渗漏和恢复肠道菌群失调的作用。
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引用次数: 0
Production of Functional White Bread Enriched with Various Dietary Fibers with High Consumer Acceptance. 富含多种膳食纤维的功能性白面包的生产,消费者接受度高。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-04 DOI: 10.1007/s11130-024-01267-7
Işılay Yılmaz, Mehmet Sertaç Özer, Halef Dizlek

Fiber-enriched breads are inferior to wheat flour (white) breads in terms of volume, taste, and textural characteristics. The aim of this study was to produce functional dietary fiber (DF)-enriched white bread (WB) with high consumer acceptance. Wheat fiber (WF) was added to wheat flour as an insoluble fiber source (5%); polydextrose and inulin were used as soluble fibers at three different concentrations (2, 4, and 6%) individually or in combination. Their effects on dough rheology (farinograph and extensograph properties) and bread properties (volume, baking loss, moisture, texture, color, sensory, and crumb-grain characteristics) were investigated. The addition of WF, polydextrose and inulin had significant effects on dough rheology and bread properties. Hardness, chewiness, cell density, and DF content of breads were generally increased, whereas volume yield, baking loss, moisture and porosity values decreased with increasing DF concentration. The bread with the highest DF content (PD6IN6) contained 7.1 times more DF than WB. According to the results of sensory analysis, except for the sample with 6% concentration of polydextrose and inulin, all the other breads had very high overall acceptance values. Although the breads produced in the study have a high fiber content, their important quality characteristics (moisture content, volume yield, cell density, color values and sensory properties) are similar to those of WB. As a result, the overall quality characteristics of bread made with different dietary fibers were maintained, and functional WB enriched with DF were produced with high consumer acceptance.

富含纤维的面包在体积、味道和质地方面都不如小麦粉(白)面包。本研究的目的是生产功能性膳食纤维(DF)丰富的白面包(WB),具有较高的消费者接受度。将小麦纤维(WF)作为不溶性纤维源(5%)添加到小麦粉中;以三种不同浓度(2、4和6%)的聚葡萄糖和菊粉作为可溶性纤维单独或联合使用。研究了它们对面团流变学(面粉特性和拉伸特性)和面包特性(体积、烘烤损失、水分、质地、颜色、感官和面包屑颗粒特性)的影响。面粉、聚葡萄糖和菊粉的添加对面团流变学和面包性能有显著影响。随着DF浓度的增加,面包的硬度、嚼劲、细胞密度和DF含量普遍增加,而体积产率、烘烤损失、水分和孔隙率均降低。DF含量最高的面包(PD6IN6) DF含量是WB的7.1倍。感官分析结果显示,除聚葡萄糖和菊粉浓度为6%的样品外,其余面包的总体接受值均非常高。虽然研究中生产的面包纤维含量高,但其重要的质量特性(含水量、体积产率、细胞密度、颜色值和感官性能)与WB相似。从而保持了不同膳食纤维制成的面包的整体品质特征,生产出的富含DF的功能性WB具有较高的消费者接受度。
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引用次数: 0
Protein-based Emulsion Hydrogels and Their Application in the Development of Sustainable Food Products. 基于蛋白质的乳液水凝胶及其在可持续食品开发中的应用。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-01 Epub Date: 2024-08-13 DOI: 10.1007/s11130-024-01214-6
Alweera Ashfaq, Khwaja Osama, Owais Yousuf, Kaiser Younis

Consumers have become more conscious of their diet, resulting in an increased demand for low-calorie and nutrient-rich food. Therefore, finding alternative ways to develop food products with improved nutritional values has become necessary without compromising the textural and sensorial properties. In the last few years, emulsion gels have gained much popularity for oil structuring, delivery of bioactive compounds, and development of nutritious food products. Protein-stabilized emulsion hydrogels have the most significant potential to be utilized in the food industry as they contain natural ingredients that help with clean label tags. Different gelation methods can be used to fabricate emulsion gels depending on the requirements of end products. Emulsion hydrogels' rheological, textural, mechanical, and structural properties can be modified by altering their composition, oil concentration, gelation method, and gelling environment, such as pH, temperature, etc. This review addresses using protein-based emulsion gels to develop novel food products with reduced-calorie and nutrition-rich content.

消费者越来越注重自己的饮食,因此对低热量和营养丰富的食品的需求越来越大。因此,有必要在不影响质地和感官特性的前提下,寻找其他方法来开发营养价值更高的食品。在过去几年中,乳液凝胶在油脂结构、生物活性化合物的输送以及营养食品的开发方面受到了广泛欢迎。蛋白质稳定乳液水凝胶在食品工业中的应用潜力最大,因为它们含有有助于清洁标签的天然成分。根据最终产品的要求,可采用不同的凝胶化方法制造乳液凝胶。乳液水凝胶的流变、质地、机械和结构特性可通过改变其成分、油浓度、凝胶化方法和胶凝环境(如 pH 值、温度等)来改变。本综述将探讨如何利用基于蛋白质的乳液凝胶来开发热量低、营养丰富的新型食品。
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引用次数: 0
Influence of Plant Phenology on Chemical Composition of Monarda fistulosa L. Organs and their Bioactive Properties. 植物物候对 Monarda fistulosa L. 器官化学成分及其生物活性特性的影响
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-01 Epub Date: 2024-09-26 DOI: 10.1007/s11130-024-01238-y
Łukasz Gontar, Anna Geszprych, Monika Sitarek-Andrzejczyk, Ewa Osińska

Monarda fistulosa L. above-ground organs, collected at three phases of plant phenology, were investigated as potential raw materials for application in the food industry. They were evaluated regarding essential oil (EO) content, composition, and antimicrobial activity, as well as characteristics of phenolic fractions and antioxidant properties, which may determine health benefits and potential use in food preservation. The dominant constituent of leaf EO was carvacrol. In the inflorescence EO carvacrol content was especially high at the full flowering phase (45.12%), while during the fruit setting phase its content was lower than that of p-cymene (39.75%) and thymoquinone (25.04%). In the agar dilution test, leaf and inflorescence EOs inhibited the growth of the six tested microorganisms at the concentration range of 0.156-0.625 µL/mL. Leaves collected at the vegetative phase of plant growth were characterised by the highest content of rosmarinic acid and didymin. Inflorescences harvested during the flowering of plants were rich in linarin. Flavonoid content was highly correlated with antioxidant activity of extracts. Due to these properties, M. fistulosa extracts and essential oils could be used in the food industry as natural preservatives or antioxidants, thereby contributing to the development of safer and more sustainable food products.

研究人员对植物物候学三个阶段采集的 Monarda fistulosa L. 地上茎进行了调查,以确定其作为食品工业应用的潜在原料。对它们的精油(EO)含量、成分、抗菌活性以及酚类组分的特征和抗氧化特性进行了评估,这可能会决定其对健康的益处以及在食品保存中的潜在用途。叶环氧乙烷的主要成分是香芹酚。花序环氧乙烷中的香芹酚含量在盛花期特别高(45.12%),而在坐果期则低于对伞花烃(39.75%)和胸腺醌(25.04%)。在琼脂稀释试验中,叶片和花序环氧乙烷在 0.156-0.625 µL/mL 的浓度范围内抑制了六种受试微生物的生长。在植物生长的无性阶段采集的叶片中,迷迭香酸和地迪明的含量最高。在植物开花期采集的花序中含有丰富的亚麻仁素。类黄酮含量与提取物的抗氧化活性高度相关。由于这些特性,M. fistulosa 的提取物和精油可在食品工业中用作天然防腐剂或抗氧化剂,从而有助于开发更安全、更可持续的食品。
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引用次数: 0
Effect of Partial Replacement of Wheat Flour by Flour from Extruded Sunflower Seed Kernels on Muffins Quality. 用挤压葵花籽仁面粉部分替代小麦粉对松饼质量的影响
IF 4.3 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-01 Epub Date: 2024-09-02 DOI: 10.1007/s11130-024-01232-4
Iryna Tsykhanovska, Olena Stabnikova, Mykola Riabchykov, Tetiana Lazarieva, Natalia Korolyova

The use of new types of raw materials to improve the quality and nutritional value of products is an important trend in flour confectionery. Flour from extruded sunflower seed kernels (FESSK), the by-product of oil production, was used as a new ingredient in muffin formulation. Analysis of physicochemical and nutritional properties of muffins prepared with FESSK, which was added in the amounts of 5, 10, and 15% to the total weight of mixture of wheat and rye flours, as well as their sensory evaluation, were performed. According to the sensory evaluation, the muffins with FESSK had a pleasant, nutty and sunflower aroma, and the best results were shown by muffins with 10% of FESSK. Addition of FESSK, 10%, led to an increase of the content of protein by 24.7%, fat by 16.9%, fiber by 23.3%, ash by 16.9%, and a decrease of content of total carbohydrates by 5.2% and sugars by 16.2%. Enriched muffins had improved texture characteristics, particularly, smooth, crack-free surface, soft, and elastic crumb with well-developed porosity and small, evenly distributed, thin-walled pores. The FESSK could be recommended as an ingredient for improving the nutritional and technological properties of flour confectionery products.

使用新型原材料来提高产品的质量和营养价值是面粉糖果的一个重要趋势。挤压葵花籽仁面粉(FESSK)是油脂生产的副产品,被用作松饼配方的新原料。在小麦粉和黑麦粉混合物的总重量中分别添加 5%、10% 和 15%的葵花籽仁面粉,对用葵花籽仁面粉配制的松饼的理化和营养特性进行了分析,并对其进行了感官评价。根据感官评价,添加了 FESSK 的松饼具有令人愉悦的坚果和向日葵香味,其中添加了 10% FESSK 的松饼效果最佳。添加 10%的 FESSK 后,蛋白质含量增加了 24.7%,脂肪增加了 16.9%,纤维增加了 23.3%,灰分增加了 16.9%,总碳水化合物含量减少了 5.2%,糖分减少了 16.2%。富集后的松饼具有更好的质地特性,尤其是表面光滑、无裂纹、松软、有弹性、孔隙度发达、孔隙小且分布均匀、壁薄。建议将 FESSK 作为一种配料,用于改善面粉糕点产品的营养和技术特性。
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引用次数: 0
Gastrointestinal Health Benefits of Sorghum Phenolics. 高粱酚类化合物对胃肠道健康的益处。
IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI: 10.1007/s11130-024-01230-6
Ibtesam Sleem, Dmitriy Smolensky, Vermont Dia

Sorghum is considered a promising food security crop and remarkable rich source of bioactive components including phenolic acids, flavonoids, and tannins. Sorghum phenolics exhibited numerous protective effects against multiple chronic diseases. However, there is no review of the effects of sorghum phenolics on gastrointestinal (GI) health. Specifically, recent studies have highly suggested that sorghum phenolics can maintain gastrointestinal homeostasis and enhance microbial diversity and richness. Furthermore, sorghum phenolics showed GI anticancer effects in both in vitro and in vivo studies against colorectal and esophageal cancers. Treatment of GI related human cancer cell lines stimulated apoptosis and suppressed proliferation. Sorghum intake and extracts treatments reduced intestinal oxidative stress and inflammatory mediators in human and in vivo studies. In addition, understanding the role and mechanisms underlying gastrointestinal health benefits of sorghum phenolics is crucial to determine treatment strategies of different GI diseases.

高粱被认为是一种前景广阔的粮食安全作物,也是生物活性成分(包括酚酸、类黄酮和单宁酸)的显著丰富来源。高粱酚类物质对多种慢性疾病具有保护作用。然而,目前还没有关于高粱酚类物质对胃肠道(GI)健康影响的综述。具体而言,最近的研究高度表明,高粱酚类物质可以维持胃肠道的平衡,并提高微生物的多样性和丰富度。此外,高粱酚类物质在体外和体内研究中都显示出对大肠癌和食道癌的胃肠道抗癌作用。对与胃肠道相关的人类癌细胞株的处理可刺激细胞凋亡,抑制细胞增殖。在人体和体内研究中,高粱摄入量和提取物处理可减少肠道氧化应激和炎症介质。此外,了解高粱酚类物质对胃肠道健康有益的作用和机制,对于确定不同胃肠道疾病的治疗策略至关重要。
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引用次数: 0
Isolation of an α-glucosidase Inhibitor from Houttuynia cordata Thunb. and Its In vitro and In vivo Hypoglycemic Bioactivity. 从Houttuynia cordata Thunb.中分离出一种α-葡萄糖苷酶抑制剂及其体内外降血糖生物活性。
IF 4.3 2区 农林科学 Q2 CHEMISTRY, APPLIED Pub Date : 2024-12-01 Epub Date: 2024-08-12 DOI: 10.1007/s11130-024-01217-3
Pei Cao, Shiyin Xiang, Shixin Liu, Yun Feng, Xinyue Zhang, Qin Wu, Jianjun Hou, Huan Liu, Dan Cheng, Xixia Liu

The Houttuynia cordata Thunb. belongs to the Saururaceae family and is a well-known medicine and food homologous plant. Herein, the isolation of an α-glucosidase inhibitor from Houttuynia cordata Thunb. and characterization of its in vitro and in vivo hypoglycemic bioactivities are reported. We optimized the extraction conditions and isolated neochlorogenic acid (nCGA), which has α-glucosidase inhibitory activity from Houttuynia cordata Thunb. for the first time. nCGA competed with glucose for the α-glucosidase binding site, with a 50% inhibitory concentration (IC50) of 0.711 mg/mL. In vivo experiments in zebrafish showed that effects of nCGA on blood glucose varied by its concentrations. In particular, 4 mg/L nCGA significantly decreased the blood glucose level and inhibited effects of α-glucosidase in zebrafish. This work provides a theoretical basis for the extraction of hypoglycemic active ingredients from Houttuynia cordata Thunb. and a foundation for the development of natural and effective α-glucosidase inhibitors.

蕺菜(Houttuynia cordata Thunb.)属于金牛星科,是一种著名的药食同源植物。本文报道了从蕺菜中分离出一种α-葡萄糖苷酶抑制剂,并对其体内外降血糖生物活性进行了表征。nCGA 与葡萄糖竞争α-葡萄糖苷酶结合位点,50%的抑制浓度(IC50)为 0.711 mg/mL。斑马鱼体内实验表明,nCGA 对血糖的影响因浓度而异。其中,4 毫克/升 nCGA 能显著降低斑马鱼的血糖水平,并抑制α-葡萄糖苷酶的作用。这项工作为从蕺菜中提取降血糖活性成分提供了理论依据,也为开发天然有效的α-葡萄糖苷酶抑制剂奠定了基础。
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Plant Foods for Human Nutrition
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