Abstract: Fluorescence lifetime imaging microcopy (FLIM) is successfully used to image the intracellular fluorescent coenzymes NAD(P)H and FAD+. The redox state of these coenzymes is a parameter which helps to reveal the metabolic status of living cells and tissues. However, metabolic reactions are strongly dependent on the intracellular oxygen level. One promising optical method to monitor oxygen in biomedical samples is phosphorescence lifetime imaging microscopy (PLIM). PLIM is based on oxygen-dependent quenching of the phosphorescence of so-called “phosphors”. In this way, PLIM enables measurement of the oxygen partial pressure (pO2) within living cells. This review describes the FLIM and PLIM approaches used in biomedical research, drawing particular attention to the techniques of simultaneous FLIM and PLIM, which provide correlative imaging of both the fluorescence lifetime of metabolic coenzymes and pO2-sensitive phosphorescence lifetime.
{"title":"FLIM and PLIM in biomedical research – An innovative way to combine autofluorescence and oxygen measurements","authors":"S. Kalinina, A. Rück","doi":"10.1515/plm-2016-0026","DOIUrl":"https://doi.org/10.1515/plm-2016-0026","url":null,"abstract":"Abstract: Fluorescence lifetime imaging microcopy (FLIM) is successfully used to image the intracellular fluorescent coenzymes NAD(P)H and FAD+. The redox state of these coenzymes is a parameter which helps to reveal the metabolic status of living cells and tissues. However, metabolic reactions are strongly dependent on the intracellular oxygen level. One promising optical method to monitor oxygen in biomedical samples is phosphorescence lifetime imaging microscopy (PLIM). PLIM is based on oxygen-dependent quenching of the phosphorescence of so-called “phosphors”. In this way, PLIM enables measurement of the oxygen partial pressure (pO2) within living cells. This review describes the FLIM and PLIM approaches used in biomedical research, drawing particular attention to the techniques of simultaneous FLIM and PLIM, which provide correlative imaging of both the fluorescence lifetime of metabolic coenzymes and pO2-sensitive phosphorescence lifetime.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80723368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. V. Bashenow, I. Motus, Sergey N. Skornyakow, A. V. Neretin, Natalya V. Rayewskaya, Ilia A. Dyachkow, Roman B. Berdnikow, Lyudmila A. Golubewa
Abstract Objective: A first experience of using a Nd:YAG laser with a wavelength of 1318 nm for limited forms of pulmonary tuberculosis surgery is reported. Subjects and methods: During the period from June 2013 to August 2015 a total of 32 patients (19 men and 13 women) underwent surgery for tuberculous granulomas. The average age of the patients was 37.3 years (median, 36 years; range, 18–61 years). Resections of granulomas were carried out using the laser alone (25 patients) or in combination with suturing devices (seven patients). Results: Overall 39 tuberculomas were removed from the 32 patients. The biggest growth measured 2.4×2.2×3.1 cm, while the smallest measured 0.5×0.5×0.5 cm. Overall, the average surgery time was 57 min (median, 50 min; range, 25–115 min). In the combined resection subgroup (using the laser and staplers), the average surgery time was 75 min (median, 50 min; range, 45–115 min). In the subgroup where lung resection was carried out using the laser alone, the average surgery time was 52 min (median, 45 min; range 25–95 min). Total blood loss during surgery was 53 ml (median, 45 ml; range 10–150 ml), 87 ml (median, 50 ml; range 15–150 ml) in the combined lung resection subgroup and 44 ml (median, 35 ml; range, 10–100 ml) in the laser only resection subgroup. Morphological analysis revealed the following results: a high level of tubercular activity was maintained in 12/39 (31%) resected tuberculous granulomas, a moderate level of tubercular activity was detected in 11/39 (28%) resected masses and remitting activity was identified in 16/39 (41%) resected masses. Along the laser resection line of the lung the edge of a tuberculous growth was found in 10/39 cases (26%). The average post-operative duration of pleural drainage was 3.9 days (median, 3 days; range, 2–9 days) in total, 4.1 days (median, 5 days; range, 2–6 days) in the combined subgroup and 3.8 days (median, 3 days; range, 2–9 days) in the laser-only subgroup. The number of post-operative complications assessed according to the Clavien-Dindo scale was two out of 32 patients (6.2%), with two cases of residual pleural cavities. Post operative mortality was 0%. Conclusion: Our first experience of using the Nd:YAG laser with a wavelength of 1318 nm for the resection of limited forms of tuberculosis demonstrated a high level of efficacy and excellent aero- and hemostatic properties with a low rate of post-operative complications even though the differences between the combined resection and laser-only subgroups were not statistically significant (p>0.05). However, the method is applicable in surgery of limited forms of lung tuberculosis in different variants, performing pure laser resections and as an additional method for other types of lung resections.
{"title":"Use of a 1318 nm Nd:YAG laser for the resection of limited forms of pulmonary tuberculosis","authors":"A. V. Bashenow, I. Motus, Sergey N. Skornyakow, A. V. Neretin, Natalya V. Rayewskaya, Ilia A. Dyachkow, Roman B. Berdnikow, Lyudmila A. Golubewa","doi":"10.1515/plm-2016-0020","DOIUrl":"https://doi.org/10.1515/plm-2016-0020","url":null,"abstract":"Abstract Objective: A first experience of using a Nd:YAG laser with a wavelength of 1318 nm for limited forms of pulmonary tuberculosis surgery is reported. Subjects and methods: During the period from June 2013 to August 2015 a total of 32 patients (19 men and 13 women) underwent surgery for tuberculous granulomas. The average age of the patients was 37.3 years (median, 36 years; range, 18–61 years). Resections of granulomas were carried out using the laser alone (25 patients) or in combination with suturing devices (seven patients). Results: Overall 39 tuberculomas were removed from the 32 patients. The biggest growth measured 2.4×2.2×3.1 cm, while the smallest measured 0.5×0.5×0.5 cm. Overall, the average surgery time was 57 min (median, 50 min; range, 25–115 min). In the combined resection subgroup (using the laser and staplers), the average surgery time was 75 min (median, 50 min; range, 45–115 min). In the subgroup where lung resection was carried out using the laser alone, the average surgery time was 52 min (median, 45 min; range 25–95 min). Total blood loss during surgery was 53 ml (median, 45 ml; range 10–150 ml), 87 ml (median, 50 ml; range 15–150 ml) in the combined lung resection subgroup and 44 ml (median, 35 ml; range, 10–100 ml) in the laser only resection subgroup. Morphological analysis revealed the following results: a high level of tubercular activity was maintained in 12/39 (31%) resected tuberculous granulomas, a moderate level of tubercular activity was detected in 11/39 (28%) resected masses and remitting activity was identified in 16/39 (41%) resected masses. Along the laser resection line of the lung the edge of a tuberculous growth was found in 10/39 cases (26%). The average post-operative duration of pleural drainage was 3.9 days (median, 3 days; range, 2–9 days) in total, 4.1 days (median, 5 days; range, 2–6 days) in the combined subgroup and 3.8 days (median, 3 days; range, 2–9 days) in the laser-only subgroup. The number of post-operative complications assessed according to the Clavien-Dindo scale was two out of 32 patients (6.2%), with two cases of residual pleural cavities. Post operative mortality was 0%. Conclusion: Our first experience of using the Nd:YAG laser with a wavelength of 1318 nm for the resection of limited forms of tuberculosis demonstrated a high level of efficacy and excellent aero- and hemostatic properties with a low rate of post-operative complications even though the differences between the combined resection and laser-only subgroups were not statistically significant (p>0.05). However, the method is applicable in surgery of limited forms of lung tuberculosis in different variants, performing pure laser resections and as an additional method for other types of lung resections.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84462476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gobbo, G. Ottaviani, K. Rupel, M. Biasotto, A. Guglielmi
Abstract Background: Patients suffering from anal cancer have to undergo different anticancer therapies, including radiotherapy. Radiodermatitis represents a debilitating side effect of radiotherapy, often leading to painful lesions and a suspension of the radiotherapy treatment. No widely recognized treatment of radiodermatitis is currently available. Objective: The present report introduces an innovative technique based on the use of laser light to manage radiodermatitis of the anal region in a decisive fashion. Study design: Two cases of radiodermatitis are presented, the occurrence of which followed combined chemoradiotherapy for anal squamous cell carcinoma. Both patients complained about strong pain, severe itch and spontaneous bleeding from the lesions. In addition, they reported limitations in their daily life activities as well as a negative impact on their quality of life. Both patients received laser irradiation therapy by applying two wavelengths simultaneously (970±15 nm and 660±15 nm) for a total of six laser sessions. The chosen treatment parameters were as follows: peak power, 6 W, 50% duty cycle for 360 s; peak power, 6 W in continuous mode for another 120 s resulting in spatially averaged energy fluences of 67.5 J/cm2 and 45 J/cm2, respectively. Results: Complete healing of the lesions and symptoms was achieved within 2 weeks. Remission of the symptoms continued after 2 months with no documented side effects. Both patients managed to complete the radiotherapy sessions and reported maximum satisfaction with the treatment. Conclusions: These cases can prepare the ground to consider class-IV laser therapy, an innovative option to manage radiodermatitis with predictable effectiveness and a consequent improvement in the quality of life of the patients. Patients experiencing radiodermatitis suffer from pain accompanied by limited independence in daily activities, decreased life quality, and reduced self-esteem which often leads to depression. Resolution of radiodermatitis can result in fewer cases of radiotherapy suspension and a better outcome in the oncological treatment as well as an improved quality of life, usually very quickly.
{"title":"Can laser therapy be the answer for radiodermatitis in anal cancer patients? Two case reports","authors":"M. Gobbo, G. Ottaviani, K. Rupel, M. Biasotto, A. Guglielmi","doi":"10.1515/plm-2016-0009","DOIUrl":"https://doi.org/10.1515/plm-2016-0009","url":null,"abstract":"Abstract Background: Patients suffering from anal cancer have to undergo different anticancer therapies, including radiotherapy. Radiodermatitis represents a debilitating side effect of radiotherapy, often leading to painful lesions and a suspension of the radiotherapy treatment. No widely recognized treatment of radiodermatitis is currently available. Objective: The present report introduces an innovative technique based on the use of laser light to manage radiodermatitis of the anal region in a decisive fashion. Study design: Two cases of radiodermatitis are presented, the occurrence of which followed combined chemoradiotherapy for anal squamous cell carcinoma. Both patients complained about strong pain, severe itch and spontaneous bleeding from the lesions. In addition, they reported limitations in their daily life activities as well as a negative impact on their quality of life. Both patients received laser irradiation therapy by applying two wavelengths simultaneously (970±15 nm and 660±15 nm) for a total of six laser sessions. The chosen treatment parameters were as follows: peak power, 6 W, 50% duty cycle for 360 s; peak power, 6 W in continuous mode for another 120 s resulting in spatially averaged energy fluences of 67.5 J/cm2 and 45 J/cm2, respectively. Results: Complete healing of the lesions and symptoms was achieved within 2 weeks. Remission of the symptoms continued after 2 months with no documented side effects. Both patients managed to complete the radiotherapy sessions and reported maximum satisfaction with the treatment. Conclusions: These cases can prepare the ground to consider class-IV laser therapy, an innovative option to manage radiodermatitis with predictable effectiveness and a consequent improvement in the quality of life of the patients. Patients experiencing radiodermatitis suffer from pain accompanied by limited independence in daily activities, decreased life quality, and reduced self-esteem which often leads to depression. Resolution of radiodermatitis can result in fewer cases of radiotherapy suspension and a better outcome in the oncological treatment as well as an improved quality of life, usually very quickly.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86873059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. S. Belova, A. Orlova, I. Balalaeva, N. Antonova, A. Maslennikova, N. M. Mishina, E. Zagaynova
Abstract Objective: A flow cytometric approach is proposed to assess the hydrogen peroxide (H2O2) level under chemotherapy action separately in viable and apoptotic tumor cells. Materials and methods: For studying the involvement of H2O2 in the process of cell death, the genetically encoded fluorescent sensor HyPer2, apoptosis marker PE Annexin V and vital dye 7-AAD were employed. The approach was used for testing the capacity of two cytotoxic drugs, cisplatin and bleomycin, to change the intracellular H2O2 concentration, depending on the stage of cell death. Results: An increase in HyPer2 fluorescence has been revealed in cells undergoing apoptosis under cisplatin action. This finding indicates that accumulation of H2O2 accompanies the cisplatin-induced apoptotic reaction. HyPer2 response was also revealed in negative to PE Annexin V viable cells which can be explained either by participation of H2O2 in the earliest stages of apoptosis or in a cell response to a non-fatal injury. Under bleomycin action, neither an apoptotic reaction nor changes of fluorescence intensity HyPer2 were detected, allowing one to assume that H2O2 is not involved in the reaction of tumor cells to bleomycin. Conclusion: The proposed approach can be used for studying the mechanisms of cell death under action of any types of antitumor drugs.
{"title":"Hydrogen peroxide detection in viable and apoptotic tumor cells under action of cisplatin and bleomycin","authors":"A. S. Belova, A. Orlova, I. Balalaeva, N. Antonova, A. Maslennikova, N. M. Mishina, E. Zagaynova","doi":"10.1515/plm-2015-0047","DOIUrl":"https://doi.org/10.1515/plm-2015-0047","url":null,"abstract":"Abstract Objective: A flow cytometric approach is proposed to assess the hydrogen peroxide (H2O2) level under chemotherapy action separately in viable and apoptotic tumor cells. Materials and methods: For studying the involvement of H2O2 in the process of cell death, the genetically encoded fluorescent sensor HyPer2, apoptosis marker PE Annexin V and vital dye 7-AAD were employed. The approach was used for testing the capacity of two cytotoxic drugs, cisplatin and bleomycin, to change the intracellular H2O2 concentration, depending on the stage of cell death. Results: An increase in HyPer2 fluorescence has been revealed in cells undergoing apoptosis under cisplatin action. This finding indicates that accumulation of H2O2 accompanies the cisplatin-induced apoptotic reaction. HyPer2 response was also revealed in negative to PE Annexin V viable cells which can be explained either by participation of H2O2 in the earliest stages of apoptosis or in a cell response to a non-fatal injury. Under bleomycin action, neither an apoptotic reaction nor changes of fluorescence intensity HyPer2 were detected, allowing one to assume that H2O2 is not involved in the reaction of tumor cells to bleomycin. Conclusion: The proposed approach can be used for studying the mechanisms of cell death under action of any types of antitumor drugs.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83869432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sophie Fürst, S. Hasmüller, A. Burges, T. Pongratz, Bettina Sailer, M. Heide, R. Sroka
Abstract Background and objective: Patients suffering from uterine leiomyoma are often treated by hysterectomy or organ preserving myoma enucleation using electrosurgical, ultrasonic or radio-frequency techniques. Considerable thermal tissue damage leads to the development of scar formation which in the long term may result in complications during pregnancy. Therefore alternative techniques are required. Materials and methods: After ethical approval, human uteri myomatosus were used to investigate the interaction of 1470 nm diode laser light (Medilas D MultiBeam; Dornier MedTech Laser GmbH, Weßling, Germany) during surgical procedures (laser mode, continuous wave; laser power, 5–30 W; fiber core diameter, 600 μm; cutting velocity, 3 mm/s). Macroscopic and microscopic evaluation was performed to improve understanding of tissue ablation and coagulation processes. Additionally laser-assisted ex-vivo myoma enucleation procedures were performed to assess handling and applicability. Descriptive and correlation statistics were used for evaluation. Results: The macroscopic visible coagulation rim was calculated to be at a maximum of ~550 μm in lateral direction (superficial width), and ~300 μm in axial plane (sagittal depth). Microscopic examination of hematoxylin and eosin stained sagittal tissue slices showed a largest ablation depth of 279.1±186.8 μm and a maximum irreversibly damaged remaining tissue depth of 628.7±354.3 μm when a laser power of 30 W was applied. The ratio of the remaining tissue and ablation depth indicates that increased applied laser power results in a faster ablation than coagulation into the depth. Hands-on ex-vivo myoma enucleation at power levels of 20–25 W showed an ideal preparation situation with the potential for sufficient surface coagulation when the fiber-tissue distance is adjusted to 5–10 mm. Conclusion: Sufficient and effective ablation and coagulation of uterus myomatosus tissue and the ex-vivo myoma enucleation showed the potential of the 1470 nm diode laser as an innovative surgical tool for myoma enucleation. Based upon this study it should be clinically validated whether this procedure could become an alternative application in endoscopic surgery in gynecology.
{"title":"Ex-vivo investigation on the potential of 1470 nm diode laser light for enucleation of uterine leiomyoma","authors":"Sophie Fürst, S. Hasmüller, A. Burges, T. Pongratz, Bettina Sailer, M. Heide, R. Sroka","doi":"10.1515/plm-2015-0038","DOIUrl":"https://doi.org/10.1515/plm-2015-0038","url":null,"abstract":"Abstract Background and objective: Patients suffering from uterine leiomyoma are often treated by hysterectomy or organ preserving myoma enucleation using electrosurgical, ultrasonic or radio-frequency techniques. Considerable thermal tissue damage leads to the development of scar formation which in the long term may result in complications during pregnancy. Therefore alternative techniques are required. Materials and methods: After ethical approval, human uteri myomatosus were used to investigate the interaction of 1470 nm diode laser light (Medilas D MultiBeam; Dornier MedTech Laser GmbH, Weßling, Germany) during surgical procedures (laser mode, continuous wave; laser power, 5–30 W; fiber core diameter, 600 μm; cutting velocity, 3 mm/s). Macroscopic and microscopic evaluation was performed to improve understanding of tissue ablation and coagulation processes. Additionally laser-assisted ex-vivo myoma enucleation procedures were performed to assess handling and applicability. Descriptive and correlation statistics were used for evaluation. Results: The macroscopic visible coagulation rim was calculated to be at a maximum of ~550 μm in lateral direction (superficial width), and ~300 μm in axial plane (sagittal depth). Microscopic examination of hematoxylin and eosin stained sagittal tissue slices showed a largest ablation depth of 279.1±186.8 μm and a maximum irreversibly damaged remaining tissue depth of 628.7±354.3 μm when a laser power of 30 W was applied. The ratio of the remaining tissue and ablation depth indicates that increased applied laser power results in a faster ablation than coagulation into the depth. Hands-on ex-vivo myoma enucleation at power levels of 20–25 W showed an ideal preparation situation with the potential for sufficient surface coagulation when the fiber-tissue distance is adjusted to 5–10 mm. Conclusion: Sufficient and effective ablation and coagulation of uterus myomatosus tissue and the ex-vivo myoma enucleation showed the potential of the 1470 nm diode laser as an innovative surgical tool for myoma enucleation. Based upon this study it should be clinically validated whether this procedure could become an alternative application in endoscopic surgery in gynecology.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91196420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the publication of the first issue of the Photonics & Lasers in Medicine’s fifth volume, the editors-inchief would like to express their thanks to all who have contributed to the journal, in particular the authors, issue editors, editorial board members and reviewers. Furthermore we are very grateful to those who have supported us in our work and encouraged us in our quest to create an international forum for the publication of latest research in the field of laser medicine and biophotonics. On this occasion, we would like to renew our invitation for contributed manuscript submissions in addition to the special topics listed hereinafter. Photonics & Lasers in Medicine has a very broad and comprehensive scope. The thematic spectrum ranges from laser optical diagnostics to thermal, non-linear therapeutic procedures and applications, optical methods including light emitting diodes (LEDs), intense pulsed light (IPL), and organic light emitting diodes (OLEDs), and last, but by no means least, clinical basic research for both human and veterinary medicine. Photonics & Lasers in Medicine gives authors the opportunity to publish late stage pre-clinical studies, and perhaps more importantly, those in-vitro, ex-vivo and early-stage clinical studies which often do not find a place in clinical journals or in technologically-oriented laser/ biophotonics journals, but offer the prospect of future translation in practice. Upcoming special issues
{"title":"Upcoming issues in 2016 – Call for papers","authors":"F. Frank, L. Lilge, C. Philipp, R. Sroka","doi":"10.1515/PLM-2015-0040","DOIUrl":"https://doi.org/10.1515/PLM-2015-0040","url":null,"abstract":"With the publication of the first issue of the Photonics & Lasers in Medicine’s fifth volume, the editors-inchief would like to express their thanks to all who have contributed to the journal, in particular the authors, issue editors, editorial board members and reviewers. Furthermore we are very grateful to those who have supported us in our work and encouraged us in our quest to create an international forum for the publication of latest research in the field of laser medicine and biophotonics. On this occasion, we would like to renew our invitation for contributed manuscript submissions in addition to the special topics listed hereinafter. Photonics & Lasers in Medicine has a very broad and comprehensive scope. The thematic spectrum ranges from laser optical diagnostics to thermal, non-linear therapeutic procedures and applications, optical methods including light emitting diodes (LEDs), intense pulsed light (IPL), and organic light emitting diodes (OLEDs), and last, but by no means least, clinical basic research for both human and veterinary medicine. Photonics & Lasers in Medicine gives authors the opportunity to publish late stage pre-clinical studies, and perhaps more importantly, those in-vitro, ex-vivo and early-stage clinical studies which often do not find a place in clinical journals or in technologically-oriented laser/ biophotonics journals, but offer the prospect of future translation in practice. Upcoming special issues","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81519953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: An overview of terahertz (THz) development is presented in view of possible medical applications, including details of the current technologies which could be effectively used. Although no clinical THz technologies are currently in use, its principal applicability has been already demonstrated in skin cancer detection and treatment, dental caries detection and pharmaceutical screening. Fundamental limitations of THz studies are highlighted which have to be overcome before clinical applications can be realized.
{"title":"Status and opportunities for future use of terahertz radiation for clinical applications","authors":"C. Treviño-Palacios","doi":"10.1515/plm-2015-0017","DOIUrl":"https://doi.org/10.1515/plm-2015-0017","url":null,"abstract":"Abstract: An overview of terahertz (THz) development is presented in view of possible medical applications, including details of the current technologies which could be effectively used. Although no clinical THz technologies are currently in use, its principal applicability has been already demonstrated in skin cancer detection and treatment, dental caries detection and pharmaceutical screening. Fundamental limitations of THz studies are highlighted which have to be overcome before clinical applications can be realized.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79871929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Laser-advanced new methods for diagnostics and therapeutics","authors":"R. Sroka, L. Lilge","doi":"10.1515/plm-2015-0046","DOIUrl":"https://doi.org/10.1515/plm-2015-0046","url":null,"abstract":"","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82294670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: Clinical Laserthermia Systems AB (CLS) has developed a treatment protocol named immunostimulating interstitial laser thermotherapy (imILTCLS) which is intended to improve current treatment regimens for metastatic cancer diseases. It aims at a controlled local tumor ablation with a subsequent release of tumor specific antigens resulting in a systemic immune activation against the remaining cancer cells. The approach has its origins in the laser-induced interstitial thermotherapy procedure and is based on a proprietary and patented technique to optimize the immunological effect. CLS has gained know-how through long experience both in clinical and pre-clinical studies of the application, laser technology, thermometry, and expertise in regulatory affairs. However, CLS does not have the in-house expertise required for fiber development and production. Therefore the company has actively searched for industrial partners to collaborate with on the design and development of a fiber delivery system tailored to imILTCLS. The aim of this collaboration is to design an application-specific laser fiber that allows the treatment of a wider range of tumors with a single treatment and overcomes the limitations of currently available laser fibers, such as the limited achievable lesion sizes due the maximum power that can be used without carbonizing tissue. The present contribution gives a short overview about the current development steps within the network of developers, industry and users.
{"title":"Optical fiber solutions for laser ablation of tissue and immunostimulating interstitial laser thermotherapy – Product development in the network of developers, industry and users","authors":"Cristina Pantaleone, S. Dymling, J. Axelsson","doi":"10.1515/plm-2015-0036","DOIUrl":"https://doi.org/10.1515/plm-2015-0036","url":null,"abstract":"Abstract: Clinical Laserthermia Systems AB (CLS) has developed a treatment protocol named immunostimulating interstitial laser thermotherapy (imILTCLS) which is intended to improve current treatment regimens for metastatic cancer diseases. It aims at a controlled local tumor ablation with a subsequent release of tumor specific antigens resulting in a systemic immune activation against the remaining cancer cells. The approach has its origins in the laser-induced interstitial thermotherapy procedure and is based on a proprietary and patented technique to optimize the immunological effect. CLS has gained know-how through long experience both in clinical and pre-clinical studies of the application, laser technology, thermometry, and expertise in regulatory affairs. However, CLS does not have the in-house expertise required for fiber development and production. Therefore the company has actively searched for industrial partners to collaborate with on the design and development of a fiber delivery system tailored to imILTCLS. The aim of this collaboration is to design an application-specific laser fiber that allows the treatment of a wider range of tumors with a single treatment and overcomes the limitations of currently available laser fibers, such as the limited achievable lesion sizes due the maximum power that can be used without carbonizing tissue. The present contribution gives a short overview about the current development steps within the network of developers, industry and users.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83672023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joan González Pedrol, J. Trull, Mercè Campoy Sánchez, J. L. Romero, P. Martínez-Carpio, M. Trelles
Abstract Objective: A clinical observation of a professional tattooing procedure and the skin damage caused by the tattooing technique and the healing process of the lesions. Materials and methods: Three tattoos were performed using black, red and white ink, together with a fourth “tattoo” with the same characteristics and an identical procedure, but using no ink. The evolution of the lesions was observed over a period of 6 months using photographs and biopsies. Results: The tattoos evolved with no complications. At 72 h, full destruction of the epidermis and de-structuring of the papillary dermis were confirmed. At 3 months, an almost ad integrum restoration of the skin structure was observed with a settling of the inks on the reticular dermis. With regard to the inkless tattoo, a wound with self-limiting capillary bleeding was observed which gradually healed, leaving only a sclerotic and hyperpigmented residual lesion by month 6. Conclusion: An awareness of the tattooing procedure would lead to a better understanding of potential complications, which might arise. This study contains the description and evolution of the lesions and provides the first images of skin damage caused by contemporary professional tattooing techniques, without the masking factor of the ink.
{"title":"Clinical observation of a professional tattooing procedure and evolutionary study of the skin damage provoked","authors":"Joan González Pedrol, J. Trull, Mercè Campoy Sánchez, J. L. Romero, P. Martínez-Carpio, M. Trelles","doi":"10.1515/plm-2015-0037","DOIUrl":"https://doi.org/10.1515/plm-2015-0037","url":null,"abstract":"Abstract Objective: A clinical observation of a professional tattooing procedure and the skin damage caused by the tattooing technique and the healing process of the lesions. Materials and methods: Three tattoos were performed using black, red and white ink, together with a fourth “tattoo” with the same characteristics and an identical procedure, but using no ink. The evolution of the lesions was observed over a period of 6 months using photographs and biopsies. Results: The tattoos evolved with no complications. At 72 h, full destruction of the epidermis and de-structuring of the papillary dermis were confirmed. At 3 months, an almost ad integrum restoration of the skin structure was observed with a settling of the inks on the reticular dermis. With regard to the inkless tattoo, a wound with self-limiting capillary bleeding was observed which gradually healed, leaving only a sclerotic and hyperpigmented residual lesion by month 6. Conclusion: An awareness of the tattooing procedure would lead to a better understanding of potential complications, which might arise. This study contains the description and evolution of the lesions and provides the first images of skin damage caused by contemporary professional tattooing techniques, without the masking factor of the ink.","PeriodicalId":20126,"journal":{"name":"Photonics & Lasers in Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89821982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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