Pub Date : 2017-08-02DOI: 10.32604/phyton.2017.86.265
M. Carmona, E. Simonetti, M. E. Ravotti, M. Scandiani, A. Luque, N. Formento, F. Sautua
Soybean root and stem rots caused by soil-bornepathogens are diseases commonly found in soybean fields, and oneof the most important causes of crop losses. In the present study, themycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotiniasclerotiorum and Macrophomina phaseolina was evaluated on potatodextrose agar media (25 mL) supplemented with different concentrationsof manganese phosphite (MnPhi) diluted in water (0, 25,37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelialgrowth sensitivity was analyzed using logarithmic linear regressionanalysis. The MnPhi concentration needed to inhibit 50% of the mycelialgrowth (IC50) ranged from 105 μg/mL (Fusarium spp.) to 409μg/mL (M. phaseolina). Sclerotia were completely inhibited at 500μg/mL. The results of our study represent the first report on the directin vitro fungicidal/fungistatic action of MnPhi against fungi thatare causal agents of soil-borne diseases.
{"title":"In vitro antifungal/fungistatic activity of manganese phosphite against soybean soil-borne pathogens","authors":"M. Carmona, E. Simonetti, M. E. Ravotti, M. Scandiani, A. Luque, N. Formento, F. Sautua","doi":"10.32604/phyton.2017.86.265","DOIUrl":"https://doi.org/10.32604/phyton.2017.86.265","url":null,"abstract":"Soybean root and stem rots caused by soil-bornepathogens are diseases commonly found in soybean fields, and oneof the most important causes of crop losses. In the present study, themycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotiniasclerotiorum and Macrophomina phaseolina was evaluated on potatodextrose agar media (25 mL) supplemented with different concentrationsof manganese phosphite (MnPhi) diluted in water (0, 25,37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelialgrowth sensitivity was analyzed using logarithmic linear regressionanalysis. The MnPhi concentration needed to inhibit 50% of the mycelialgrowth (IC50) ranged from 105 μg/mL (Fusarium spp.) to 409μg/mL (M. phaseolina). Sclerotia were completely inhibited at 500μg/mL. The results of our study represent the first report on the directin vitro fungicidal/fungistatic action of MnPhi against fungi thatare causal agents of soil-borne diseases.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"365 1","pages":"265-269"},"PeriodicalIF":1.7,"publicationDate":"2017-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78125461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-31DOI: 10.32604/phyton.2017.86.007
E. Zúñiga-Mendoza, L. F. Ceja-Torres
In vitro tests were carried out, to evaluate the antagonistcapacity of a strain of Trichoderma isolated from soil in LaCienega de Chapala, Michoacan, Mexico against Sclerotium cepivorumand Sclerotium rolfsii, casual agents of onion rot, an importantdisease in Mexico and the rest of the world. Percentages of radialgrowth inhibition (PRGI) were calculated every 24 h, until a rateof antagonism was obtained according to the Bell’s scale, as well asthe percentages of inhibition of the production of sclerotia and theirparasitism. The PRGI was 17.4% against S. rolfsii and 22.2% againstS. cepivorum after 48 and 72 h, respectively. Trichoderma harzianumobtained a degree of antagonism of 1 on S. cepivorum and two againstS. rolfsii, on the Bell scale. On the other hand, T. harzianum reduced(P≤0.05) 95% the production of sclerotia of S. cepivorum and 78%that of S. rolfsii; the parasitism of the sclerotia of S. cepivorum and S.rolfsii was 91% and 54%, respectively.
{"title":"In vitro antagonism of Trichoderma harzianum on Sclerotium cepivorum Berk. and S. rolfsii Sacc., causal agents of onion rot","authors":"E. Zúñiga-Mendoza, L. F. Ceja-Torres","doi":"10.32604/phyton.2017.86.007","DOIUrl":"https://doi.org/10.32604/phyton.2017.86.007","url":null,"abstract":"In vitro tests were carried out, to evaluate the antagonistcapacity of a strain of Trichoderma isolated from soil in LaCienega de Chapala, Michoacan, Mexico against Sclerotium cepivorumand Sclerotium rolfsii, casual agents of onion rot, an importantdisease in Mexico and the rest of the world. Percentages of radialgrowth inhibition (PRGI) were calculated every 24 h, until a rateof antagonism was obtained according to the Bell’s scale, as well asthe percentages of inhibition of the production of sclerotia and theirparasitism. The PRGI was 17.4% against S. rolfsii and 22.2% againstS. cepivorum after 48 and 72 h, respectively. Trichoderma harzianumobtained a degree of antagonism of 1 on S. cepivorum and two againstS. rolfsii, on the Bell scale. On the other hand, T. harzianum reduced(P≤0.05) 95% the production of sclerotia of S. cepivorum and 78%that of S. rolfsii; the parasitism of the sclerotia of S. cepivorum and S.rolfsii was 91% and 54%, respectively.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"74 1","pages":"7-13"},"PeriodicalIF":1.7,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89527510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-24DOI: 10.32604/phyton.2017.86.112
Gustavo Carlos María Giaccio, L. Pedro, Cabria Fabian
The analysis of lateral flow runoff of superficial nutrientsand sediments from an agricultural origin and their retentionby infiltration of riparian vegetation strips are of great importancein reducing the agricultural externalities on surface water quality.The aim of this study was to relate soil infiltration to the main biophysicalproperties of riparian environments in the Pampa Australof Argentina, defined by the composition of its plant communityand the coverage of exotic species. We explored the relationships betweenhydraulic conductivity (Ks) and aboveground, litter and rootbiomasses, organic matter concentration, terrain slope, soil bulk density,texture and electrical conductivity. We determined eight floristicassociations through the classification of 65 censuses of vegetation.Three of the associations were dominated by native grasses, threeby exotic grasses and two presented a layer of exotic willow trees incombination with native and invasive grasses. The mean Ks in soilsfrom the floristic associations with trees and from the associationsof native grasses without trees were higher than in soils from theassociations of exotic grasses without trees. Significant relationshipswere found between Ks and the soil properties that are sensitive tothe influence of vegetation, suggesting that the relationships betweenfloristic composition and infiltration could be explained partly due tocausal relationships.
{"title":"Floristic associations and filtering ability of riparian vegetation strips","authors":"Gustavo Carlos María Giaccio, L. Pedro, Cabria Fabian","doi":"10.32604/phyton.2017.86.112","DOIUrl":"https://doi.org/10.32604/phyton.2017.86.112","url":null,"abstract":"The analysis of lateral flow runoff of superficial nutrientsand sediments from an agricultural origin and their retentionby infiltration of riparian vegetation strips are of great importancein reducing the agricultural externalities on surface water quality.The aim of this study was to relate soil infiltration to the main biophysicalproperties of riparian environments in the Pampa Australof Argentina, defined by the composition of its plant communityand the coverage of exotic species. We explored the relationships betweenhydraulic conductivity (Ks) and aboveground, litter and rootbiomasses, organic matter concentration, terrain slope, soil bulk density,texture and electrical conductivity. We determined eight floristicassociations through the classification of 65 censuses of vegetation.Three of the associations were dominated by native grasses, threeby exotic grasses and two presented a layer of exotic willow trees incombination with native and invasive grasses. The mean Ks in soilsfrom the floristic associations with trees and from the associationsof native grasses without trees were higher than in soils from theassociations of exotic grasses without trees. Significant relationshipswere found between Ks and the soil properties that are sensitive tothe influence of vegetation, suggesting that the relationships betweenfloristic composition and infiltration could be explained partly due tocausal relationships.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"97 1","pages":"112-123"},"PeriodicalIF":1.7,"publicationDate":"2017-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80531785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-22DOI: 10.32604/phyton.2017.86.199
C. C. Castillo-Aguilar, R. Garruña, J. Zúñiga-Aguilar, Aa Guzmán-Antonio
The Habanero pepper (Capsicum chinense) is intensivelycultivated in the Yucatan peninsula, Mexico. Because of adverseenvironmental conditions, it required seeding in germinationtrays, from which six-week-old seedlings were transplanted to thesoil. Adequate nursing and fertilization programmes were made toimprove health and vigour before seedlings transplanting. Duringseed germination, we investigated the effects of inoculation withfour plant growth-promoting rhizobacteria (PGPR) on growth,nutrient uptake and gas exchange of 8-week-old Capsicum chinenseplants. Inoculation was made with Pseudomonas sp. -P61-, Pseudomonassp. -A46-, Bacillus pumillus -R44-, and Paenibacillus polymyxa-BSP1.1-. The BSP1.1 strain produced the highest increase in plantheight (22.52 cm), leaf area (99 cm2), fresh weight of roots (0.337g) and shoots (2.83 g), and dry weights of roots (0.05 g) and shoots(0.43 g). Nitrogen uptake was similar (P>0.05) among treatments.However, seedlings inoculated with BSP1.1 had a higher accumulationthan controls when they were exposed to P and K (50 and40%, respectively). Likewise, seedlings inoculated with the BSP1.1strain improved both photosynthesis (2 μmol/m2/s) and water useefficiency (7.3 μmol CO2/mmol H2O), and decreased transpirationrate (0.27 mmol/m2/s). Thus, the inoculation with the BSP1.1 strainwas the best option to enhance growth and vigour of Capsicum chinenseplants.
{"title":"PGPR inoculation improves growth, nutrient uptake and physiological parameters of Capsicum chinense plants","authors":"C. C. Castillo-Aguilar, R. Garruña, J. Zúñiga-Aguilar, Aa Guzmán-Antonio","doi":"10.32604/phyton.2017.86.199","DOIUrl":"https://doi.org/10.32604/phyton.2017.86.199","url":null,"abstract":"The Habanero pepper (Capsicum chinense) is intensivelycultivated in the Yucatan peninsula, Mexico. Because of adverseenvironmental conditions, it required seeding in germinationtrays, from which six-week-old seedlings were transplanted to thesoil. Adequate nursing and fertilization programmes were made toimprove health and vigour before seedlings transplanting. Duringseed germination, we investigated the effects of inoculation withfour plant growth-promoting rhizobacteria (PGPR) on growth,nutrient uptake and gas exchange of 8-week-old Capsicum chinenseplants. Inoculation was made with Pseudomonas sp. -P61-, Pseudomonassp. -A46-, Bacillus pumillus -R44-, and Paenibacillus polymyxa-BSP1.1-. The BSP1.1 strain produced the highest increase in plantheight (22.52 cm), leaf area (99 cm2), fresh weight of roots (0.337g) and shoots (2.83 g), and dry weights of roots (0.05 g) and shoots(0.43 g). Nitrogen uptake was similar (P>0.05) among treatments.However, seedlings inoculated with BSP1.1 had a higher accumulationthan controls when they were exposed to P and K (50 and40%, respectively). Likewise, seedlings inoculated with the BSP1.1strain improved both photosynthesis (2 μmol/m2/s) and water useefficiency (7.3 μmol CO2/mmol H2O), and decreased transpirationrate (0.27 mmol/m2/s). Thus, the inoculation with the BSP1.1 strainwas the best option to enhance growth and vigour of Capsicum chinenseplants.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"36 1","pages":"199-204"},"PeriodicalIF":1.7,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89698320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-22DOI: 10.32604/PHYTON.2017.86.318
I. Semerdjieva, E. Yankova-Tsvetkova
Scanning electron microscope (SEM) study of the pollenand seed morphology of Peganum harmala L. and Zygophyllumfabago L. (Zygophyllaceae), in native Bulgarian populations, wascarried out. Additionally, a light microscope (LM) investigation onpollen grains was made in order to elucidate the peculiarities of themajor parameters of pollen surface and size. It was established thatpollen grains in Z. fabago are pantoporate. Exine ornamentation isreticulate. The pollen grains of P. harmala are oblate, spheroidal, elongated,oval in shape, colporate, exine striato-rugulate. The seeds of P.harmala are oblong-oval and slightly flattened at the side (incorrecttetrahedral to rhomboid), with a peaked edge, arc-curve laterally. Theseed sculpture corresponds to the Tubular to Concave-type, whichis characterized by polygonal cells and reticulate tectum. The seedsof Z. fabago are oval-rhomboid in shape. The seed sculpture correspondsto Concave to Convex-type which is characterized with polygonalcells, ranging in size, with cuticular wax folds.
用扫描电镜(SEM)研究了保加利亚本地种群中Peganum harmala L.和Zygophyllumfabago L. (zygophylllfabago L.)的花粉和种子形态。此外，为了阐明花粉表面和大小的主要参数的特殊性，对花粉颗粒进行了光镜观察。结果表明，蚕豆属花粉粒为泛孔型。外叶纹饰网状。其花粉粒呈扁圆形、球形、长形、卵圆形、整体状、外壁纹状。种子长椭圆形，侧面略扁平(不正确的四面体到菱形)，边缘呈尖峰状，侧面呈弧形。这些种子雕刻对应于管状到凹状，其特征是多边形细胞和网状顶盖。蚕豆种子呈椭圆形菱形。种子雕刻属于凹到凸型，其特征是多角形细胞，大小不等，表皮有蜡褶皱。
{"title":"Pollen and seed morphology of Zygophylum fabago and Peganum harmala (Zygophyllaceae) from Bulgaria","authors":"I. Semerdjieva, E. Yankova-Tsvetkova","doi":"10.32604/PHYTON.2017.86.318","DOIUrl":"https://doi.org/10.32604/PHYTON.2017.86.318","url":null,"abstract":"Scanning electron microscope (SEM) study of the pollenand seed morphology of Peganum harmala L. and Zygophyllumfabago L. (Zygophyllaceae), in native Bulgarian populations, wascarried out. Additionally, a light microscope (LM) investigation onpollen grains was made in order to elucidate the peculiarities of themajor parameters of pollen surface and size. It was established thatpollen grains in Z. fabago are pantoporate. Exine ornamentation isreticulate. The pollen grains of P. harmala are oblate, spheroidal, elongated,oval in shape, colporate, exine striato-rugulate. The seeds of P.harmala are oblong-oval and slightly flattened at the side (incorrecttetrahedral to rhomboid), with a peaked edge, arc-curve laterally. Theseed sculpture corresponds to the Tubular to Concave-type, whichis characterized by polygonal cells and reticulate tectum. The seedsof Z. fabago are oval-rhomboid in shape. The seed sculpture correspondsto Concave to Convex-type which is characterized with polygonalcells, ranging in size, with cuticular wax folds.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"35 1","pages":"318-324"},"PeriodicalIF":1.7,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82793628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-05-22DOI: 10.32604/phyton.2017.86.137
Gang Jin, X. W. Tang, Y. Niu, X. Huang, T. Chen, Q. Huang, J. Zhang, R. Zhou
In plants, the mitochondrial NADH dehydrogenase(complex I) is a large protein complex transferring electrons to ubiquinone.For the nine chondriogenes encoding complex I subunits (nad1,nad2, nad3, nad4, nad4L, nad5, nad6, nad7, and nad9), an efficientstrategy for the cloning of complete coding sequences (CDSs) is important.Specific orthologous portions of untranslated regions (UTRs)were found based on multiple sequence alignments of chondriogeneorthologues encoding complex I subunits in plant species. Based onthe conservation of partial UTRs, a one-step PCR strategy was conceivedfor the cloning of CDSs of the nine chondriogene orthologues.Using this strategy, the five complete mitochondrial open readingframes (ORFs) , which encode mitochondrial NADH dehydrogenasesubunits, nad1, nad2, nad6, nad7 and nad9 respectively, were cloned inthree angiosperm species: kenaf (Hibiscus cannabinus), camphor tree(Cinnamomum camphora), and ramie (Boehmeria nivea). The fifteencloned PCR products also included 5' and 3'-UTR partial sequences.Moreover, a potential C-U RNA editing site was identified in the startcodon of kenaf nad9. In conclusion, the simple and efficient strategyavoids the use of time-consuming rapid amplification of cDNA ends(RACE) process, and facilitates the cloning mitochondrial completeORFs whose 5' and 3' flanking UTR contain an orthologous regionwith some degeneracy in higher plant species.
{"title":"Evolutionarily conserved untranslated regions facilitate the cloning of complete coding sequences of chondriogenes encoding NADH dehydrogenase subunits in higher plants","authors":"Gang Jin, X. W. Tang, Y. Niu, X. Huang, T. Chen, Q. Huang, J. Zhang, R. Zhou","doi":"10.32604/phyton.2017.86.137","DOIUrl":"https://doi.org/10.32604/phyton.2017.86.137","url":null,"abstract":"In plants, the mitochondrial NADH dehydrogenase(complex I) is a large protein complex transferring electrons to ubiquinone.For the nine chondriogenes encoding complex I subunits (nad1,nad2, nad3, nad4, nad4L, nad5, nad6, nad7, and nad9), an efficientstrategy for the cloning of complete coding sequences (CDSs) is important.Specific orthologous portions of untranslated regions (UTRs)were found based on multiple sequence alignments of chondriogeneorthologues encoding complex I subunits in plant species. Based onthe conservation of partial UTRs, a one-step PCR strategy was conceivedfor the cloning of CDSs of the nine chondriogene orthologues.Using this strategy, the five complete mitochondrial open readingframes (ORFs) , which encode mitochondrial NADH dehydrogenasesubunits, nad1, nad2, nad6, nad7 and nad9 respectively, were cloned inthree angiosperm species: kenaf (Hibiscus cannabinus), camphor tree(Cinnamomum camphora), and ramie (Boehmeria nivea). The fifteencloned PCR products also included 5' and 3'-UTR partial sequences.Moreover, a potential C-U RNA editing site was identified in the startcodon of kenaf nad9. In conclusion, the simple and efficient strategyavoids the use of time-consuming rapid amplification of cDNA ends(RACE) process, and facilitates the cloning mitochondrial completeORFs whose 5' and 3' flanking UTR contain an orthologous regionwith some degeneracy in higher plant species.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":" 400","pages":"137-142"},"PeriodicalIF":1.7,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72378309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of Nitrogen, Phosphorous, Potassium and Foliar Feeding on Total Lipids and Fatty Acids of Nigella sativa L. Grown in Arid Zones","authors":"K. Khalid","doi":"10.3923/IJB.2017.52.58","DOIUrl":"https://doi.org/10.3923/IJB.2017.52.58","url":null,"abstract":"","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"28 1","pages":"52-58"},"PeriodicalIF":1.7,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83847000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-01DOI: 10.3923/IJB.2017.103.114
Krisma Lekitoo, Hans Fence Zake, Novita Panambe, Reinardus Liborius C
{"title":"Ecological and Ethnobotanical Facet of 'Kelapa Hutan' (Pandanus Spp.) and Perspectives Towards its Existence and Benefit","authors":"Krisma Lekitoo, Hans Fence Zake, Novita Panambe, Reinardus Liborius C","doi":"10.3923/IJB.2017.103.114","DOIUrl":"https://doi.org/10.3923/IJB.2017.103.114","url":null,"abstract":"","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"16 1","pages":"103-114"},"PeriodicalIF":1.7,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75100106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-01DOI: 10.3923/ijb.2017.126.133
R. Ma, Salama Mohamed El, Kholod Ali Khatta
Background and Objective: Haplophyllum tuberculatum is a valuable medicinal species with wide distribution in Mediterranean coastal desert of Egypt. It was characterized by high level of intraspecific morphological and biochemical variability. The objective of study was to assess the species status diversity for its coordinated conservation. Materials and Methods: Twenty-seven Operation Taxonomic Units (OTU’s) from nine populations were collected along the Mediterranean coastal desert of Egypt. Eighty seven macro and micro morphological characters, besides 114 molecular attributes from both seed storage proteins and RAPD were evaluated. The agglomerative cluster analysis conducted and the dissimilarity matrix analyzed through three sorting methods, average linkage UPGMA, single linkage and Ward's. Results: The OTU's from 19-21 of Abo-Tamr village were discriminated from the other populations due to a large distance between the inflorescence and the first leaf, united sepals and united stamens at base. The studied samples achieved 8 common bands and both El-Karma (OTU's 7-9) and El-Gophera villages were distinguished by the absence of both polymorphism and the band at 77 KDa. The discrete DNA products per primer ranged from 12-19 bands, the percentage of polymorphism from 24-31% and fingerprinting bands from 1-4. Conclusion: Two biotypes of H. tuberculatum can be distinguished, OTU’s from 19-21 of Abo-Tamr and the other ecotypes. The determination of the rank of these biotypes needs the study of the morphologically similar H. blanche to confirm the presence of the two species.
{"title":"Discrimination of Two Biotypes of Haplophyllum tuberculatum (Forssk.) A. Juss. (Rutaceae) by Morphology, SDS-PAGE and RAPD","authors":"R. Ma, Salama Mohamed El, Kholod Ali Khatta","doi":"10.3923/ijb.2017.126.133","DOIUrl":"https://doi.org/10.3923/ijb.2017.126.133","url":null,"abstract":"Background and Objective: Haplophyllum tuberculatum is a valuable medicinal species with wide distribution in Mediterranean coastal desert of Egypt. It was characterized by high level of intraspecific morphological and biochemical variability. The objective of study was to assess the species status diversity for its coordinated conservation. Materials and Methods: Twenty-seven Operation Taxonomic Units (OTU’s) from nine populations were collected along the Mediterranean coastal desert of Egypt. Eighty seven macro and micro morphological characters, besides 114 molecular attributes from both seed storage proteins and RAPD were evaluated. The agglomerative cluster analysis conducted and the dissimilarity matrix analyzed through three sorting methods, average linkage UPGMA, single linkage and Ward's. Results: The OTU's from 19-21 of Abo-Tamr village were discriminated from the other populations due to a large distance between the inflorescence and the first leaf, united sepals and united stamens at base. The studied samples achieved 8 common bands and both El-Karma (OTU's 7-9) and El-Gophera villages were distinguished by the absence of both polymorphism and the band at 77 KDa. The discrete DNA products per primer ranged from 12-19 bands, the percentage of polymorphism from 24-31% and fingerprinting bands from 1-4. Conclusion: Two biotypes of H. tuberculatum can be distinguished, OTU’s from 19-21 of Abo-Tamr and the other ecotypes. The determination of the rank of these biotypes needs the study of the morphologically similar H. blanche to confirm the presence of the two species.","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"95 1","pages":"126-133"},"PeriodicalIF":1.7,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90377662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-03-01DOI: 10.3923/ijb.2017.115.125
S. Agele, I. K. Oyewusi, O. Aiyelari, I. Famuwagun
{"title":"Dry Matter Production, Biomass Partitioning and Seed Setting Efficiencies in Early- and Late-rainy Season Cowpea in the Rainforest Agroecology of South-West Nigeria","authors":"S. Agele, I. K. Oyewusi, O. Aiyelari, I. Famuwagun","doi":"10.3923/ijb.2017.115.125","DOIUrl":"https://doi.org/10.3923/ijb.2017.115.125","url":null,"abstract":"","PeriodicalId":20184,"journal":{"name":"Phyton-international Journal of Experimental Botany","volume":"26 1","pages":"115-125"},"PeriodicalIF":1.7,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82176088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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