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Insights into historic and genetic relationships of diverse common lilac (Syringa vulgaris) genotypes based on whole-genome profiling 基于全基因组图谱对不同普通丁香(Syringa vulgaris)基因型的历史和遗传关系的见解
Pub Date : 2023-12-20 DOI: 10.1017/s1479262123001053
Helena Korpelainen, Leena Lindén

Common lilac (Syringa vulgaris L.) is a popular landscaping plant. Our aim was to obtain a large set of single nucleotide polymorphism (SNP) markers, to reveal the precise identities of the investigated S. vulgaris accessions, and to discover genetic relationships among them. The studied plant material included local Finnish, previously unidentified accessions, known reference cultivars, and so-called historical accessions i.e., old shrubs growing in historic cultural landscapes. We intended to verify cultivar names for some valuable local common lilac accessions and to provide insights into the history of common lilac cultivation in Finland. In the analyses, we used a set of 15,007 SNP markers. First, polymorphic information contents were calculated (mean 0.190, range 0.012–0.500 per marker). Then, to investigate genetic relationships among genotypes, a phylogenetic tree was constructed, and a principal coordinate analysis was conducted. A Bayesian analysis of population structure was performed to determine the number and distribution of genetic clusters among samples. Genetic marker data combined with existing historical and phenotypic knowledge revealed novel information on the unidentified cultivars and on the genetic relationships among studied accessions and solved the arrival and early history of common lilac in Finland. Overall, such comprehensive genomic characterization and deep understanding of genetic relationships of S. vulgaris can be used when utilizing present cultivars and developing new ones in future breeding programs.

普通丁香(Syringa vulgaris L.)是一种广受欢迎的景观植物。我们的目的是获得大量的单核苷酸多态性(SNP)标记,揭示所调查的普通丁香品种的确切身份,并发现它们之间的遗传关系。所研究的植物材料包括芬兰本地的、以前未确定的登录品种、已知的参考栽培品种以及所谓的历史登录品种,即生长在历史文化景观中的古老灌木。我们的目的是验证一些珍贵的本地普通丁香品种的栽培品种名称,并深入了解芬兰普通丁香的栽培历史。在分析中,我们使用了一组 15,007 个 SNP 标记。首先,我们计算了每个标记的多态信息含量(平均值为 0.190,范围为 0.012-0.500)。然后,为了研究基因型之间的遗传关系,构建了一棵系统发生树,并进行了主坐标分析。对种群结构进行了贝叶斯分析,以确定样本间遗传集群的数量和分布。遗传标记数据与现有的历史和表型知识相结合,揭示了未确定栽培品种的新信息和所研究品种间的遗传关系,并解开了普通丁香进入芬兰和早期历史的谜团。总之,在未来的育种计划中,利用现有栽培品种和开发新栽培品种时,可以利用这种全面的基因组表征和对普通丁香遗传关系的深入了解。
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引用次数: 0
Diversity analyses of chickpea (Cicer arietinum L.) through agro-morphological traits 通过农业形态特征分析鹰嘴豆(Cicer arietinum L.)的多样性
Pub Date : 2023-12-20 DOI: 10.1017/s1479262123001065
Nasser Eddine Dehbaoui, Laurent Gentzbittel, Jean Jacques Drevon, Mohamed Lazali

The limited genetic resource of chickpea (Cicer arietinum L.) is one of the most critical issue impeding its improvement and production. Collection and characterization of chickpea for agronomic traits is prerequisite to build a successful breeding programme through expanding chickpea's genepool to fulfil the needs of farmers and breeders, such as grain size and yield which supply food to a world rising population. To reach such an objective, numerous collection missions were carried out in Algeria, covering the northern agro-ecological environments, this allows us to gather 56 national and international genotypes that report a phenotypic variability and may provide a valuable diversity of useful features. Characterization experiments of 19 agro-morphological traits were set up at the Technical Institute of Field Crops over two successive seasons 2020 and 2021. All traits were statistically significant and the cluster analysis classified the genotypes into three major group, where local and improved accessions revealed an interesting outcome in terms of seed yield and resistance to Ascochyta blight. In general, the current study's findings clearly demonstrated that plant and seed morphological characteristics could be used to distinguish and identify chickpea genotypes. More often, it might participate as a source of variation in breeding programmes to meet the desirable improvement targets.

鹰嘴豆(Cicer arietinum L.)遗传资源有限是阻碍其改良和生产的最关键问题之一。收集鹰嘴豆的农艺性状并对其进行特征描述,是通过扩大鹰嘴豆的基因库来满足农民和育种者的需求(如为全球不断增加的人口提供粮食的粒度和产量),从而建立一个成功的育种计划的先决条件。为了实现这一目标,我们在阿尔及利亚开展了多次采集工作,采集范围涵盖北部农业生态环境,从而收集到 56 个国内和国际基因型,这些基因型具有表型变异性,并可能提供有用特征的宝贵多样性。2020 年和 2021 年连续两季,在大田作物技术研究所对 19 个农业形态特征进行了表征实验。所有性状均具有统计学意义,聚类分析将基因型分为三大类,其中本地和改良品种在种子产量和抗穗轴锈病方面表现出令人感兴趣的结果。总之,目前的研究结果清楚地表明,植物和种子形态特征可用于区分和识别鹰嘴豆基因型。通常情况下,它可以作为育种计划的变异源,以达到理想的改良目标。
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引用次数: 0
Evaluation of indigenous and exotic soybean accessions for yield, resistance to frog-eye leaf spot and yellow mosaic virus diseases 评估本土和外来大豆品种的产量、对蛙眼叶斑病和黄曲霉病毒病的抗性
Pub Date : 2023-12-15 DOI: 10.1017/s1479262123000941
Anuradha Bhartiya, Vangala Rajesh, J. P. Aditya, Jeevan B., Sanjay Gupta, Lakshmi Kant, Hemlata Joshi, S. P. Mehtre, H. N. Devi, S. Jaybhay, M. K. Karnwal, Vennampally Nataraj, Nita Khandekar
Soybean is a major source of vegetable oil and protein worldwide. Globally, India is among the top five producers where soybean is a major oilseed grown under diverse agro-climatic conditions by small and marginal farmers. The present study aims to identify soybean varieties with higher yield levels, resistance to pestdiseases and adaptability to climatic fluctuations. One hundred and twenty-five (125) indigenous and exotic soybean germplasm accessions and five checks were evaluated and characterized for eight agro-morphological traits at five testing locations and also screened for frog-eye leaf spot (FLS) and yellow mosaic virus (YMV) diseases under hot-spot locations during the rainy season. A wide range of variability was observed among accessions for days to 50% flowering (39–59), plant height (41–111 cm), number of nodes/plant (10–30), pod clusters/plant (14–39), number of pods/plant (40–102), days to maturity (96–115), grain yield/plant (4.89–16.54 g) and 100-seed weight (6.02–13.72 g). Among various traits, 100-seed weight (0.45), number of pods/plant (0.60) and number of pod clusters/plant (0.38) were found to be major yield-contributing traits as they exhibited highly significant correlation with grain yield/plant. Principal components PCI and PCII with eigen value >1 accounted for 42.66 and 27.08% of the total variation, respectively. Accessions G24 (EC 393222) from Taiwan and G40 (IMP-1) from the USA belonging to cluster IV were found promising for multiple yield traits and JS 20–38 from cluster III for earliness as per cluster analysis. GGE biplot average environment coordination (AEC) view revealed that the accessions viz., G11 (EC 333872), G2 (EC 251506) and G47 (TNAU-S-55) were the best performing stable genotypes in terms of grain yield/plant across locations. Twelve accessions had a high level of resistance against both FLS and YMV diseases under natural hot-spot conditions which can be utilized as promising donors in the soybean breeding programme.
大豆是全球植物油和蛋白质的主要来源。印度是全球五大大豆生产国之一,大豆是小农和边缘化农民在不同农业气候条件下种植的主要油籽。本研究旨在确定产量更高、抗虫害和适应气候波动的大豆品种。研究人员在五个测试地点对 125 份本土和外来大豆种质登录品和五份对照品进行了评估,对其八个农业形态特征进行了鉴定,并在雨季热点地区对蛙眼叶斑病(FLS)和黄镶嵌病毒(YMV)病进行了筛查。在 50%开花天数(39-59 天)、株高(41-111 厘米)、节数/株(10-30 节)、荚丛/株(14-39 个)、荚数/株(40-102 个)、成熟天数(96-115 天)、谷物产量/株(4.89-16.54 克)和百粒重(6.02-13.72 克)方面,各参试品种之间的差异很大。在各种性状中,百粒重(0.45)、荚数/株(0.60)和荚簇数/株(0.38)被认为是主要的产量贡献性状,因为它们与谷物产量/株表现出极显著的相关性。特征值为 1 的主成分 PCI 和 PCII 分别占总变异的 42.66% 和 27.08%。根据聚类分析,来自台湾的 G24(EC 393222)和来自美国的 G40(IMP-1)属于第 IV 聚类,在多个产量性状方面表现良好,而来自第 III 聚类的 JS 20-38 则在早熟性方面表现良好。GGE 双图平均环境协调图(AEC)显示,G11(EC 333872)、G2(EC 251506)和 G47(TNAU-S-55)是各地谷物产量/株表现最好的稳定基因型。在自然热点条件下,有 12 个品系对 FLS 和 YMV 病害具有较高的抗性,可作为大豆育种计划中的有前途的供体。
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引用次数: 0
Morphometric diversity of some Nigerian accessions of Bambara groundnut (Vigna subterranea) 尼日利亚一些班巴拉落花生(Vigna subterranea)品种的形态多样性
Pub Date : 2023-12-13 DOI: 10.1017/s1479262123001028
Opeyemi Tunde Osundare, Oluwole Benjamin Akinyele, Alexander C. Odiyi, Michael Abberton, Olaniyi Ajewole Oyatomi, Rajneesh Paliwal

This study evaluated the morphological diversity of some Nigerian accessions of Bambara groundnut. Field experiments were conducted in Nigeria in two locations and the phenotypic variation of Bambara groundnut accessions evaluated in 3 years (2017–2020) using a randomized complete block design. Twenty-eight quantitative and 10 qualitative traits were observed. Twenty-three out of the 28 quantitative morphological traits showed significant differences. TVSu-589 (28.85) and TVSu-670 (28.57) had the highest plant height; TVSu-572 (121.52), TVSu-271 (113.10) and TVSu-336 (104.15) had the highest 100-seed weight. Genetic variations were also observed in seed colour, terminal leaflet shape, petiole colour and eye pattern. Principal component analysis showed PC1 constituting 23.36% and PC2 constituting 15.76% of the total variation, while the first eight principal components with eigenvalues ≥1 revealed 77.28% of the total variation. Cluster analysis grouped the accessions into four groups. Based on yield data in this study, accessions TVSu-594, TVSu-350, TVSu-336, TVSu-1242, TVSu-129, TVSu-14, TVSu-179, TVSu-2100, TVSu-261 and TVSu-589 were the best for yield and are recommended for further evaluation to improve yield. This study showed that a wide range of diversity exists in Bambara groundnut of Nigerian origin that could be useful for further utilization of genetic resources and improvement.

本研究评估了尼日利亚一些班巴拉花生品种的形态多样性。该研究在尼日利亚的两个地点进行了田间试验,并采用随机完全区组设计对班巴拉落花生品种的表型变异进行了为期三年(2017-2020 年)的评估。共观察到 28 个数量性状和 10 个质量性状。28 个定量形态性状中有 23 个表现出显著差异。TVSu-589 (28.85)和 TVSu-670 (28.57)株高最高;TVSu-572 (121.52)、TVSu-271 (113.10)和 TVSu-336 (104.15)百粒重最高。在种子颜色、顶生小叶形状、叶柄颜色和眼纹方面也观察到遗传变异。主成分分析表明 PC1 和 PC2 分别占总变异的 23.36% 和 15.76%,而特征值≥1 的前 8 个主成分则占总变异的 77.28%。聚类分析将加入的品种分为四组。根据产量数据,TVSu-594、TVSu-350、TVSu-336、TVSu-1242、TVSu-129、TVSu-14、TVSu-179、TVSu-2100、TVSu-261 和 TVSu-589 的产量最佳,建议进一步评估以提高产量。这项研究表明,尼日利亚原产的班巴拉花生存在广泛的多样性,可用于进一步利用遗传资源和改良。
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引用次数: 0
Discovery of male sterility from an interspecific cross between Jatropha curcas and J. integerrima 发现麻疯树和麻疯树种间杂交的雄性不育现象
Pub Date : 2023-12-11 DOI: 10.1017/s1479262123001016
Premroedee Phithakhongsa, Patcharin Tanya, Anuruck Arunyanark, Chamnanr Phetcharat, Narathid Muakrong, Peerasak Srinives

Jatropha (Jatropha curcas L.) is a shrub that produces non-food oil and can potentially be used for biodiesel production. An interspecific cross was made between J. curcas and peregrina (J. integerrima) to increase genetic diversity. Interestingly, male sterility was observed in the F2 population. Out of the 445 F2 plants, five, namely, ms-1 to ms-5, exhibited male sterility, characterized by unopened and distorted stamens without pollen. The parental jatropha, peregrina, F1 and F2 had fertile pollen grain rates of 90.61%, 96.39%, 81.46% and 75.39%, respectively. To verify the fertility of the pistils in the male sterile plants, they were pollinated through selfing, opening and hand crossing with fertile pollen. All of the ms lines experienced seed abortion with or without fruit, except for ‘ms-5’, which produced seed.

麻疯树(Jatropha curcas L.)是一种生产非食用油的灌木,可用于生产生物柴油。为了增加遗传多样性,麻疯树和麻风树(J. integerrima)进行了种间杂交。有趣的是,在 F2 群体中观察到了雄性不育现象。在 445 株 F2 植株中,有 5 株(即 ms-1 至 ms-5)表现出雄性不育,其特征是雄蕊未开放且扭曲,没有花粉。亲本麻风树、百日草、F1 和 F2 的可育花粉粒率分别为 90.61%、96.39%、81.46% 和 75.39%。为了验证雄性不育植株雌蕊的可育性,对它们进行了自交、开放和可育花粉人工杂交授粉。除了 "ms-5 "能结出种子外,所有 ms 品系都出现了有果或无果的种子流产现象。
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引用次数: 0
Evaluation of garlic genotypes for yield performance and stability using GGE biplot analysis and genotype by environment interaction 利用 GGE 双图分析和基因型与环境的相互作用评估大蒜基因型的产量表现和稳定性
Pub Date : 2023-12-07 DOI: 10.1017/s1479262123000904
Fekadu Gebretensay Mengistu, Gizachew Atinafu Mossie, Getachew Tabor Fita

In Ethiopia, the low productivity of garlic is largely due to a shortage of high yielding and stable varieties. To address this issue, thirteen garlic genotypes were evaluated for yield stability and performance across six different environments in central and southeast Ethiopia. The genotypes were analysed using Additive Main effects and Multiplicative Interaction (AMMI) and genotype-by-environment interaction (G × E) methods. Results showed that the genotypes differed significantly for bulb yield performance and morphological traits, with G-020/03 and G-054/03 producing the highest bulb yield with 25.39% and 18.39% yield advantages over the check variety, Kuriftu, respectively. Furthermore, G-020/03 demonstrated better yield stability across most environments, while G-054/03 exhibited specific adaptability. GGE biplot analysis confirmed that these two genotypes were among the three winning genotypes in terms of yield performance and relative stability. Therefore, G-020/03 and G-054/03 have been selected as candidate varieties for release, potentially improving garlic productivity in Ethiopia.

在埃塞俄比亚,大蒜产量低的主要原因是缺乏高产、稳定的品种。为解决这一问题,我们对埃塞俄比亚中部和东南部六个不同环境下的十三个大蒜基因型的产量稳定性和表现进行了评估。采用加性主效应和乘性交互作用(AMMI)以及基因型与环境交互作用(G × E)方法对这些基因型进行了分析。结果表明,各基因型在球茎产量表现和形态特征方面差异显著,其中 G-020/03 和 G-054/03 的球茎产量最高,分别比对照品种 Kuriftu 高出 25.39% 和 18.39%。此外,G-020/03 在大多数环境中表现出更好的产量稳定性,而 G-054/03 则表现出特殊的适应性。GGE 双图分析证实,这两个基因型在产量表现和相对稳定性方面是三个优胜基因型之一。因此,G-020/03 和 G-054/03 被选为候选发布品种,有望提高埃塞俄比亚的大蒜产量。
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引用次数: 0
Fine mapping and discovery of candidate genes for rind trait in watermelon 西瓜果皮性状候选基因的精细定位与发现
Pub Date : 2023-12-01 DOI: 10.1017/s1479262123000813
Zhi Qiang Wang, Huiying Du, Cheng Li
Fine mapping and discovery of watermelon rind trait candidate genes are of great significance for modern watermelon breeding and development. In this study, we used the high-resolution genetic mapping and genome-wide genetic variation detection technology, combined with genome survey and sequencing technology, to locate and discover the candidate genes for rind traits of star watermelon varieties ‘Su XuanBai’ and ‘SHLX21’. Firstly, we identified a total of eight quantitative trait loci (QTLs) related to watermelon rind traits on chromosome 6. Secondly, a total of 208,240 single nucleotide polymorphisms and 75,345 small Indels (insertions/deletions) were detected in the two parents by high-coverage re-sequencing, respectively. Based on the genetic variation of the two parents and combined with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis using the planta database, the QTL region was reduced to 0.02 Mb. Finally, we identified the six potential regulatory factors for watermelon rind traits using real-time quantitative PCR. In conclusion, our results revealed the fine localization of candidate genes for watermelon rind traits and the successful discovery of candidate genes for regulating watermelon rind traits, which is of importance for watermelon rind traits and breeding-improved watermelon varieties.
西瓜皮性状候选基因的精细定位和发现对现代西瓜育种和发育具有重要意义。本研究采用高分辨率遗传作图和全基因组遗传变异检测技术,结合基因组调查和测序技术,定位和发现了明星西瓜品种‘苏宣白’和‘SHLX21’的外皮性状候选基因。首先,在6号染色体上鉴定出8个与西瓜皮性状相关的数量性状位点(qtl)。其次,通过高覆盖重测序,在亲本中分别检测到208,240个单核苷酸多态性和75,345个小indel(插入/缺失)。根据两亲本的遗传变异,结合基因本体(GO)和京都基因与基因组百科全书(KEGG)数据库的分析,将QTL区域缩小到0.02 Mb。最后,利用实时定量PCR技术鉴定了6个影响西瓜皮性状的潜在调控因子。总之,我们的研究结果揭示了西瓜皮性状候选基因的精细定位,成功发现了调控西瓜皮性状的候选基因,这对西瓜皮性状和西瓜品种的选育具有重要意义。
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引用次数: 0
Participatory tree selection and fruit morphological characterization of Phoebe cooperiana (U.N Kanjilal ex A. Das) in the Eastern Himalayas of India 印度东喜马拉雅地区菲比(Phoebe cooperiana)的参与式树选择和果实形态特征
Pub Date : 2023-12-01 DOI: 10.1017/s1479262123000989
Jasmine Pabin, Nicolee Lyngdoh, Shivani Dobhal, Kalidas Upadhyaya, Mongjam Bishwapati Devi
Selection of desirable phenotypes and characterization of variability in economically important traits are essential for domestication of indigenous fruit trees (IFTs). Currently, participatory tree selection is a widely accepted approach in IFT domestication wherein farmers' knowledge and preferences are included in tree selection processes. In Arunachal Pradesh, India, fruits of Phoebe cooperiana are extensively consumed by people and traded across the state. In this study, we employed a selection index to screen trees for superior fruit traits from a base population of 45 farmer-identified trees of P. cooperiana in Arunachal Pradesh. Based on fruit diameter length ratio, pulp fruit ratio and yield, 19 trees were selected for superior fruit traits. The per cent improvement for 10 fruit and seed traits ranged from 0.16 to 14.56% compared to the base population. Significant variation for all fruit and seed parameters was observed among the 19 trees with coefficient of variation values ranging between 1.92% for fruit diameter and 8.26% for seed weight. There was significant positive association between economic traits including fruit weight with pulp weight, fruit diameter length ratio with fruit weight and pulp thickness with pulp weight. Principal component analysis revealed that variability was largely contributed by fruit weight (0.49), pulp weight (0.46), seed weight (0.45) and fruit diameter (0.42). Cluster analysis grouped phenotypes into seven groups with no clear clustering of individuals from the same area. The study highlights the significance of participatory tree selection approach and the benefits of individual tree selection in capturing wider variation and locating extreme phenotypes in IFT domestication.
理想表型的选择和重要经济性状变异的鉴定是乡土果树驯化的关键。目前,参与式树木选择是一种被广泛接受的IFT驯化方法,其中农民的知识和偏好被纳入树木选择过程。在印度**,菲比的果实被人们广泛食用,并在全州范围内进行交易。在本研究中,我们以**地区农民鉴定的45棵木为基础群体,采用选择指数对果树的果实性状进行筛选。根据果径长比、果浆比和产量等指标,筛选出果实性状优良的19株。与基础群体相比,10个果实和种子性状的改良率为0.16% ~ 14.56%。结果表明,果实和种子各参数变异显著,果实直径变异系数为1.92%,种子重变异系数为8.26%。果实质量与果肉质量、果实直径长比与果肉质量、果肉厚度与果肉质量呈显著正相关。主成分分析表明,变异主要由果实质量(0.49)、果肉质量(0.46)、种子质量(0.45)和果实直径(0.42)贡献。聚类分析将表型分为7组,同一地区的个体没有明确的聚类。该研究强调了参与式树选择方法的重要性,以及个体树选择在捕获更广泛的变异和定位IFT驯化中极端表型方面的益处。
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引用次数: 0
Analysis of agro-morphological variability and inter-trait relationships in Ghanaian groundnut (Arachis hypogaea L.) accessions 加纳花生(arachhis hypogaea L.)种质的农业形态变异及性状间关系分析
Pub Date : 2023-12-01 DOI: 10.1017/s1479262123000916
Richard Adu Amoah, Charles Nelimor, Bright Adu Gymafi, Richard Boampong, Collins Yeboah Osei, Abraham Yeboah, Victoria Sackey, Eric Osafo Ansah, Samuel Awuah, Abednego Opoku Mensah, Yaw Kwateng, Lawrence Misa Aboagye
Germplasm characterization and evaluation are essential for the genetic improvement of crops. In this study, a collection of 204 groundnut accessions including 13 checks held by Plant Genetic Resources Research Institute, Ghana were evaluated under optimal conditions for 2 years. The objectives were to characterize the groundnut collection using 18 qualitative and 14 quantitative traits and to determine the relationships between the traits. Combined analysis of variance based on augmented design revealed significant differences (P < 0.05) among the accessions for majority of the traits. Results of principal component analysis showed that all the quantitative traits were relevant in discriminating the accessions. Primary seed colour was the most diverse qualitative trait based on Shannon diversity index (n = 0.77). Frequency distribution analysis showed predominance of decumbent growth habit and pale tan seeds. Cluster analysis using qualitative traits identified five major groups of accessions and three each based on quantitative traits and joint analysis of quantitative and qualitative traits. Number of main branches (NMB) and pod width (PW) appeared the most important traits with positive contribution to yield based on correlation and path coefficient analysis. Ten promising trait-specific accessions were identified for earliness along with desirable pod and seed traits. GH 9672 and GH 9665 were identified as promising for higher grain and fodder yield. Five accessions (GH 9833, GH 9829, GH 9830, GH 9835 and GH 9750) produced ~5–30% more grain yield than the best check. This study underscored ample phenotypic variation that would ultimately be exploited for genetic improvement of groundnut.
种质资源的鉴定与评价是作物遗传改良的基础。本研究以加纳植物遗传资源研究所持有的204份花生材料(包括13份检查)为研究对象,在最优条件下进行了2年的评价。目的是利用18个定性性状和14个定量性状对花生种质资源进行表征,并确定性状间的相互关系。基于增强设计的联合方差分析显示显著差异(P <0.05)。主成分分析结果表明,所有数量性状对品种的鉴别均有相关性。Shannon多样性指数(n = 0.77)表明,原色是最具多样性的品质性状。频率分布分析显示,平卧生长习性和浅褐色种子占优势。利用质量性状聚类分析确定了5个主要类群,数量性状聚类分析确定了3个主要类群。相关分析和通径分析显示,主枝数和荚果宽度对产量的贡献为正,是最重要的性状。初步鉴定出10个具有较好荚果和种子性状的品种。GH 9672和GH 9665具有较高的籽粒和饲料产量。5个品系(GH 9833、GH 9829、GH 9830、GH 9835和GH 9750)的籽粒产量比最佳对照高出5-30%。该研究强调了丰富的表型变异,最终将用于花生的遗传改良。
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引用次数: 0
Cladistics groupings of the active breeding cocoa genetic resources of Nigeria for physicochemical and nutraceutical traits 尼日利亚活跃育种可可遗传资源理化和营养性状的分类
Pub Date : 2023-11-30 DOI: 10.1017/s1479262123000977
Daniel B. Adewale, Oluwayemisi O. Adeigbe
Preference for functional and nutritious food capable of meeting consumers' demand and health is on the increase. The present preliminary study seeks to assess physico-chemical and nutraceutical diversity in the cocoa bean powder of 77 genotypes present in four Nigerian cocoa field banks. Twenty ripe pods/genotypes in each of the four active breeding field banks at the Cocoa Research Institute of Nigeria (CRIN), Ibadan, Nigeria were utilized. Composite beans from the 20 pods of each genotype were singly fermented, sun-dried and milled. Duplicate samples of the powder of each genotype were analysed for physico-chemical and nutraceutical components. Twenty-one polymorphic variables distinguished the 77 cocoa genotypes. Grouping by dendogram identified four clusters, three differently and uniquely captured 100% of the genotype membership in the local clone, international clone and the regional varieties field bank but 86% of the genotypes in the hybrid trial field bank were grouped in cluster I. Prominent traits with highest values in each clusters were: protein, pH, Ca, K and Fe (Cluster I), Zn and Mg (Cluster II), crude fat and P (Cluster III) and crude fibre, ash, theobromine, flavonoids and caffeine (Cluster IV). Exploitable diversity for nutritional quality improvement is present in the active breeding and working collections of Nigerian cocoa field banks.
消费者对既能满足需求又能保证健康的功能性、营养性食品的偏好日益增加。目前的初步研究旨在评估尼日利亚四个可可田库中77种基因型可可粉的物理化学和营养多样性。利用了尼日利亚伊巴丹市尼日利亚可可研究所(CRIN)四个活跃育种田间库中各20个成熟荚果/基因型。从每种基因型的20个豆荚中提取复合豆进行单独发酵、晒干和研磨。对各基因型粉末的重复样品进行理化和营养成分分析。21个多态变量区分了77个可可基因型。通过树形图分组鉴定出4个聚类,其中3个不同且独特地捕获了本地无性系、国际无性系和区域品种田间库中100%的基因型隶属度,而杂交试田库中86%的基因型隶属于聚类i。蛋白质、pH值、Ca、K和Fe(第一类)、Zn和Mg(第二类)、粗脂肪和P(第三类)以及粗纤维、灰分、可可碱、类黄酮和咖啡因(第四类)。尼日利亚可可田银行的活跃育种和工作收集中存在可用于改善营养质量的可开发多样性。
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Plant Genetic Resources
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