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Correction of plasma fat-soluble vitamin levels by blood lipids in elderly patients with coronary heart disease 通过血脂校正老年冠心病患者的血浆脂溶性维生素水平
IF 1.9 Q3 Health Professions Pub Date : 2024-05-01 DOI: 10.1016/j.plabm.2024.e00404
Xin-Yu Wang , Xiangzhi Liu , Chengliang Zhen , Nannan Tian , Haina Ma , Menghan Wang , Li Wang

This study aims to investigate the correlation between plasma fat-soluble vitamin levels and blood lipid in elderly patients with coronary heart disease (CHD). A total of 120 participants were enrolled, including 60 CHD patients and 60 controls without CHD. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify plasma levels of vitamins A, D3, E, and K. Data analysis was conducted using the statistical analysis system module of MetaboAnalyst 5.0. The CHD group showed significantly higher levels of plasma total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) but not high-density lipoprotein cholesterol (HDL-C) compared to controls. The CHD group exhibited significantly higher plasma levels of VA and VE, positively correlating with TC, TG, and LDL-C. After adjusted by TG levels, the CHD group had significantly lower plasma levels of VA and VE, negatively correlating with TC, TG, and LDL-C. The CHD group also had significantly lower concentrations of VD3, independent of TG modification, compared to controls. VD3 negatively correlated with TC, TG, and LDL-C. Elderly individuals with CHD display abnormal blood lipid metabolism, and fat-soluble vitamins adjusted by TG levels can more accurately and timely response to implicit fat-soluble vitamins deficiency in CHD patients.

本研究旨在探讨老年冠心病(CHD)患者血浆脂溶性维生素水平与血脂之间的相关性。研究共招募了 120 名参与者,包括 60 名冠心病患者和 60 名非冠心病对照组。采用液相色谱-串联质谱法(LC-MS/MS)定量检测血浆中维生素A、D3、E和K的含量。与对照组相比,CHD组的血浆总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)水平明显较高,但高密度脂蛋白胆固醇(HDL-C)水平并不高。心脏病组的血浆 VA 和 VE 水平明显较高,与 TC、TG 和 LDL-C 呈正相关。根据 TG 水平进行调整后,CHD 组的血浆 VA 和 VE 水平明显较低,与 TC、TG 和 LDL-C 呈负相关。与对照组相比,冠心病组的 VD3 浓度也明显较低,与 TG 的变化无关。VD3 与总胆固醇、总胆固醇和低密度脂蛋白胆固醇呈负相关。患有冠心病的老年人血脂代谢异常,根据 TG 水平调整脂溶性维生素可以更准确、更及时地应对冠心病患者隐性脂溶性维生素缺乏的问题。
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引用次数: 0
Analytical performance evaluation of hemoglobin A1c on an ARKRAY HA-8160 analyzer with newly-developed mobile phase buffer 使用新开发的流动相缓冲液在 ARKRAY HA-8160 分析仪上进行血红蛋白 A1c 分析性能评估
IF 1.9 Q3 Health Professions Pub Date : 2024-05-01 DOI: 10.1016/j.plabm.2024.e00414
Yuan Yu , Xiaoyun Zhang , Kai Lin

Background

Most glycated hemoglobin A1c (HbA1c) analytical reagents used were obtained from the analyzer's manufacturer. However, clinical laboratories need more choices for HbA1c analytical reagents to overcome the limitations of dedicated reagents for special analyzers. We developed new mobile phase buffers as HbA1c diagnostic reagents and evaluated their analytical performance for the HbA1c assay.

Methods

Different mobile phase buffers used as HbA1c diagnostic reagents were prepared using different concentrations of sodium salts. According to the Clinical and Laboratory Standards Institute (CLSI) recommendation guidelines, the analytical performances of the newly developed mobile phase buffers were evaluated on an ARKRAY HA-8160 Analyzer. Both quality controls and clinical blood samples were used in these experiments. To assess the quality of the newly developed mobile phase buffers, precision, accuracy, linearity, carryover, interference, bias, correlation with commercial reagents, and stability were analyzed.

Results

The CVs of intra-assay precision and interassay precision of quality control and clinical.

There were fewer than 1.00 % blood sample assays using the newly developed mobile phase buffer. The RDs of accuracy were less than 1.00 %. Linearity: R2 = 0.9998 in the concentration range of 4.40%–17.30 %. Carryover: 0.00 %. Reagent comparison revealed that the Pearson regression equation was Y = 0.9884x+0.05692 (R2 = 0.9977), and the Bland-Altman mean difference was −0.02650 % (CI: −0.2121 %–0.1591 %) between the two analytical reagents. Stability was also acceptable within 12 months. This mobile phase buffer showed good anti-interference ability.

Conclusion

The newly developed mobile phase buffers demonstrated good analytical performance and were suitable for clinical HbA1c assays on an ARKRAY HA-8160 Analyzer.

背景大多数糖化血红蛋白 A1c(HbA1c)分析试剂都是从分析仪制造商那里获得的。然而,临床实验室需要更多的 HbA1c 分析试剂来克服专用分析仪试剂的局限性。我们开发了新的流动相缓冲液作为 HbA1c 诊断试剂,并评估了它们在 HbA1c 检测中的分析性能。根据临床和实验室标准协会(CLSI)的推荐指南,在 ARKRAY HA-8160 分析仪上对新开发的流动相缓冲液的分析性能进行了评估。实验中使用了质量控制和临床血液样本。为了评估新开发的流动相缓冲液的质量,对其精密度、准确度、线性、携带、干扰、偏差、与商业试剂的相关性和稳定性进行了分析。准确度的 RD 小于 1.00%。线性度在 4.40%-17.30 % 的浓度范围内,R2 = 0.9998。迁移率0.00 %.试剂比较显示,两种分析试剂的皮尔逊回归方程为 Y = 0.9884x+0.05692 (R2 = 0.9977),Bland-Altman 平均差为 -0.02650 % (CI: -0.2121 %-0.1591 %)。12 个月内的稳定性也是可以接受的。结论新开发的流动相缓冲液具有良好的分析性能,适合在 ARKRAY HA-8160 分析仪上进行临床 HbA1c 检测。
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引用次数: 0
An alternative method for inferring Pandy's test using cerebrospinal fluid total protein 利用脑脊液总蛋白推断潘迪试验的另一种方法
IF 1.9 Q3 Health Professions Pub Date : 2024-05-01 DOI: 10.1016/j.plabm.2024.e00411
Liu Dong , Xiaoqing Wang , Qianqian Xu, Ruoshui Cao, Xuan Deng, Jian Chen, Haoqin Jiang

Background

Pandy's test is used to assess the globulin level in cerebrospinal fluid (CSF). As a semi-quantitative manual method, the practicality and clinical value of Pandy's test has been challenged.

Objective

We tend to summarize the relationship between CSF total protein (CSF-TP) quantification and Pandy's results, providing a formula to estimate Pandy's results merely by CSF-TP value.

Methods

This retrospective study involved 1090 cases hospitalized in Huashan Hospital during 1/1/2023 to 20/4/2023. All samples were divided into six group based on their Pandy's results. Their corresponding CSF-TP quantitative results were subsequently analyzed and summarized. Another 364 patients were also gathered for verification.

Results

The turbidity of samples won't affect examiners'ocular inspection and interpretation of Pandy's tests in positive groups. The results of Pandy's tests can be deduced based on CSF-TP quantitative results according to following rules: CSF-TP quantitative results 0–614 mg/L for Pandy negative (−), 615–1322 mg/L for extremely weak positive (±), 1323–2953 mg/L for weak positive (1+), 2954–6561 mg/L for medium positive results (2+), 6562–13007 mg/L for strong positive results (3+) and CSF-TP results >13007 for strongest positive (4+). The quantitative range above was experimentally verified as effective and correct by calculating the agreement rate through another 364 samples and the R ratio of each Pandy group was greater than 90 %.

Conclusion

There is an excellent correlation between CSF-TP and Pandy's test. Therefore, CSF-TP quantification test through PROT Slides can be used to infer the results of Pandy's test to accelerate the abolish of this traditional manual test.

背景潘迪试验用于评估脑脊液(CSF)中的球蛋白水平。方法本回顾性研究涉及华山医院 2023 年 1 月 1 日至 2023 年 4 月 20 日住院的 1090 例患者。所有样本根据潘迪结果分为六组。随后对其相应的 CSF-TP 定量结果进行分析和总结。结果在阳性组中,样本的浑浊度不会影响检查人员的眼部检查和对 Pandy's 检测结果的解读。根据 CSF-TP 定量结果,可按以下规则推断 Pandy's 试验的结果:CSF-TP 定量结果 0-614 mg/L 为潘迪阴性(-),615-1322 mg/L 为极弱阳性(±),1323-2953 mg/L 为弱阳性(1+),2954-6561 mg/L 为中等阳性(2+),6562-13007 mg/L 为强阳性(3+),CSF-TP 结果 >13007 为最强阳性(4+)。通过计算另外 364 份样本的一致率,实验验证了上述定量范围的有效性和正确性,各 Pandy 组的 R 比值均大于 90%。因此,通过 PROT 幻灯片进行 CSF-TP 定量试验可用于推断 Pandy 试验的结果,从而加快取消这种传统的人工试验。
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引用次数: 0
Refractory hypoxia and saturation gap in a COVID-19 patient 一名 COVID-19 患者的难治性缺氧和饱和度缺口
IF 1.9 Q3 Health Professions Pub Date : 2024-04-18 DOI: 10.1016/j.plabm.2024.e00395
Abidah Mobarak , Subashini C. Thambiah , Ana Daliela Masiman , Intan Nureslyna Samsudin , Yin Ye Lai

Acquired methemoglobinemia, predominantly due to oxidizing medications occurs when heme iron in hemoglobin is oxidized from ferrous to ferric ion and binds oxygen irreversibly leading to functional anemia, cyanosis, and tissue hypoxia. We report a case of a 60-year-old man with multiple comorbidities who was diagnosed with coronavirus disease 2019 (COVID-19) and developed methemoglobinemia after consumption of prescribed supplements. He presented with dyspnea and cyanosis. An oxygen saturation gap with characteristic chocolate-brown arterial blood indicated methemoglobinemia. Outsourced methemoglobin (MetHb) was increased at 9.0%. Despite aggressive intervention, he succumbed to his illness. In this case, we discuss the pathophysiology of why some individuals, especially the elderly with COVID-19 are more susceptible to develop methemoglobinemia after possibly being exposed to oxidizing agents. Laboratory methods for assessing oxygen saturation, including pulse oximetry, arterial blood gas and co-oximetry are examined in relation to this case. The importance of considering a diagnosis of methemoglobinemia based on clinical and biochemical findings although MetHb assay or co-oximetry are not readily available is also emphasized.

当血红蛋白中的血红素铁由亚铁离子氧化为铁离子,并与氧不可逆地结合,导致功能性贫血、紫绀和组织缺氧时,就会发生获得性高铁血红蛋白血症,这主要是由氧化性药物引起的。我们报告了一例 60 岁男性病例,他患有多种并发症,被诊断为冠状病毒病 2019(COVID-19),并在服用处方补品后出现高铁血红蛋白血症。他出现呼吸困难和紫绀。血氧饱和度出现差距,动脉血呈特征性的巧克力棕色,表明出现了高铁血红蛋白血症。外包高铁血红蛋白(MetHb)升高至 9.0%。尽管采取了积极的干预措施,他还是因病去世。在这个病例中,我们讨论了为什么有些人,尤其是患有 COVID-19 的老年人在可能接触氧化剂后更容易患高铁血红蛋白血症的病理生理学原因。结合本病例研究了评估血氧饱和度的实验室方法,包括脉搏血氧仪、动脉血气和联合血氧仪。此外,还强调了根据临床和生化检查结果考虑高铁血红蛋白血症诊断的重要性,尽管 MetHb 检测或联合氧饱和度测定法并不容易获得。
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引用次数: 0
Application of a clamshell isothermal nucleic acid amplification analyzer in the detection of lower respiratory tract bacteria 蛤壳式等温核酸扩增分析仪在检测下呼吸道细菌中的应用
IF 1.9 Q3 Health Professions Pub Date : 2024-04-16 DOI: 10.1016/j.plabm.2024.e00394
Guanbin Zhang , Xiaoying Lin , Wenkun Mu , Jun Luo , Yiyuan Xu , Chicheng Song , Jiang Li

Objectives

The clamshell isothermal nucleic acid amplification analyzer RTisochip-S, a next-generation instrument featuring improved structural design, enhanced functional integration, reduced cost, and increased portability, was assessed for its suitability in clinical respiratory pathogens detection.

Methods

The certificated detection kit for lower respiratory tract bacteria (LRTB-kit) was applied to evaluate the performance of RTisochip-S via sensitivity, specificity, and repeatability analysis. The clinical specimens, including 51 sputum specimens and 10 bronchoalveolar lavage fluid specimens, were simultaneously detected on both RTisochip-S and a certificated reference instrument (RTisochip-A) to assess the consistency.

Results

The results indicated that RTisochip-S fulfills the sensitivity, specificity, and repeatability requirements of the LRTB-Kit, and the results of clinical specimens on the two instruments were consistent.

Conclusions

RTisochip-S is satisfying the clinical detection of respiratory pathogens while enhancing portability and compactness, making it more well-suited for point-of-care testing (POCT) applications.

方法 应用下呼吸道细菌认证检测试剂盒(LRTB-kit),通过灵敏度、特异性和重复性分析评估 RTisochip-S 的性能。临床标本包括 51 份痰标本和 10 份支气管肺泡灌洗液标本,同时用 RTisochip-S 和认证参考仪器(RTisochip-A)进行检测,以评估其一致性。结果结果表明,RTisochip-S 符合 LRTB-Kit 的灵敏度、特异性和重复性要求,两种仪器对临床标本的检测结果一致。
{"title":"Application of a clamshell isothermal nucleic acid amplification analyzer in the detection of lower respiratory tract bacteria","authors":"Guanbin Zhang ,&nbsp;Xiaoying Lin ,&nbsp;Wenkun Mu ,&nbsp;Jun Luo ,&nbsp;Yiyuan Xu ,&nbsp;Chicheng Song ,&nbsp;Jiang Li","doi":"10.1016/j.plabm.2024.e00394","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00394","url":null,"abstract":"<div><h3>Objectives</h3><p>The clamshell isothermal nucleic acid amplification analyzer RTisochip-S, a next-generation instrument featuring improved structural design, enhanced functional integration, reduced cost, and increased portability, was assessed for its suitability in clinical respiratory pathogens detection.</p></div><div><h3>Methods</h3><p>The certificated detection kit for lower respiratory tract bacteria (LRTB-kit) was applied to evaluate the performance of RTisochip-S via sensitivity, specificity, and repeatability analysis. The clinical specimens, including 51 sputum specimens and 10 bronchoalveolar lavage fluid specimens, were simultaneously detected on both RTisochip-S and a certificated reference instrument (RTisochip-A) to assess the consistency.</p></div><div><h3>Results</h3><p>The results indicated that RTisochip-S fulfills the sensitivity, specificity, and repeatability requirements of the LRTB-Kit, and the results of clinical specimens on the two instruments were consistent.</p></div><div><h3>Conclusions</h3><p>RTisochip-S is satisfying the clinical detection of respiratory pathogens while enhancing portability and compactness, making it more well-suited for point-of-care testing (POCT) applications.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000404/pdfft?md5=cf27d7a272776a666a30c260483b2917&pid=1-s2.0-S2352551724000404-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140618087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative analysis of salivary cortisol measurements using different assay methods in relation to serum-free cortisol measurement 使用不同检测方法进行唾液皮质醇测量与无血清皮质醇测量的比较分析
IF 1.9 Q3 Health Professions Pub Date : 2024-04-09 DOI: 10.1016/j.plabm.2024.e00393
Anna Lee , Sooah Jang , Sanghoo Lee , Hyun-Kyung Park , In-Young Kim , Ryunsup Ahn , Jeong-Ho Seok , Kyoung-Ryul Lee

Objectives

Salivary cortisol reflects the biologically active form of serum cortisol, offering a noninvasive evaluation method for the diurnal rhythm of the hypothalamic-pituitary-adrenal (HPA) axis. While liquid chromatography-tandem mass spectrometry (LC-MS/MS) is known for its specificity, immunoassays (IA) are commonly used because of their simplicity. This study aimed to assess the performance of salivary cortisol measurement using both IA and LC-MS/MS in comparison to serum-free cortisol measurement.

Methods

Assay results for 188 saliva and 94 serum samples from 47 participants were analyzed. Salivary samples collected at different time points were analyzed using IA and LC-MS/MS. Serum samples were analyzed for cortisol, cortisol-binding globulin, and free cortisol. The statistical analyses included correlations and method comparisons.

Results

The diurnal salivary cortisol profiles exhibited a comparable circadian rhythm pattern; however, the concentrations measured using IA were consistently higher than those measured using LC-MS/MS. The correlation analysis revealed robust associations among salivary cortisol (IA), salivary cortisol (LC-MS/MS), and serum-free cortisol levels (LC-MS/MS). However, the method comparison revealed a systematic bias between IA and LC-MS/MS in salivary cortisol measurement.

Conclusions

This study contributes to the ongoing debate on assay techniques by affirming the suitability of IA and LC-MS/MS for salivary cortisol measurement to assess dynamic changes in HPA axis activity. The identified systematic bias emphasizes the importance of selecting methods based on specific research or clinical requirements.

目的涎皮质醇反映了血清皮质醇的生物活性形式,为下丘脑-垂体-肾上腺(HPA)轴的昼夜节律提供了一种无创评估方法。液相色谱-串联质谱法(LC-MS/MS)以其特异性著称,而免疫测定法(IA)则因其简便性而常用。本研究旨在评估使用免疫测定法和 LC-MS/MS 测量唾液皮质醇的性能,并与无血清皮质醇测量进行比较。采用 IA 和 LC-MS/MS 分析了在不同时间点采集的唾液样本。对血清样本进行了皮质醇、皮质醇结合球蛋白和游离皮质醇分析。结果昼夜唾液皮质醇曲线表现出相似的昼夜节律模式;但是,用 IA 测得的浓度始终高于用 LC-MS/MS 测得的浓度。相关性分析表明,唾液皮质醇(IA)、唾液皮质醇(LC-MS/MS)和无血清皮质醇水平(LC-MS/MS)之间存在很强的相关性。结论 该研究肯定了唾液皮质醇测量中 IA 和 LC-MS/MS 在评估 HPA 轴活动动态变化方面的适用性,从而为目前关于检测技术的争论做出了贡献。发现的系统性偏差强调了根据特定研究或临床要求选择方法的重要性。
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引用次数: 0
Influence of plasma collection tubes on N-glycome in human blood samples 血浆采集管对人体血液样本中 N-糖蛋白的影响
IF 1.9 Q3 Health Professions Pub Date : 2024-03-01 DOI: 10.1016/j.plabm.2024.e00383
Zejian Zhang , Xiangyi Cui , Nan Zhou , Lisi Zhu , Yuxiang Zhi , Shuyang Zhang

Background and aims

Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.

Materials and methods

We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.

Results

Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits.

Conclusion

Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.

背景和目的定量分析血浆N-甘氨酸是一种很有前景的确定疾病生物标志物的方法。本研究旨在调查使用不同抗凝剂的采血管对血浆 N-lycome的影响。材料和方法我们使用一种稳健的质谱方法对两组健康志愿者(组群 1,n = 16;组群 2,n = 53)的血浆 N-lycome进行了分析。结果主要成分分析表明,根据采血管的不同,血样的聚类也不同。配对比较显示,在 82 个量化的 N-糖特征中,EDTA 和肝素血浆在 55 个特征上存在显著差异;在 82 个特征中,EDTA 和柠檬酸血浆在 62 个特征上存在显著差异。这些差异涵盖了各种 N-聚糖特征,包括聚糖类型、硅烷基化、半乳糖基化、岩藻糖基化和双链化。与 EDTA 血浆相比,柠檬酸盐和肝素血浆中 N 聚糖的变化趋势基本一致。在相关性分析(EDTA 与肝素;EDTA 与枸橼酸盐)中,大多数 N-糖特征的皮尔逊相关系数始终高于 0.7。结论样品基质的变化会影响血浆 N-糖蛋白的测量,在糖化学项目中比较来自不同血浆收集管的样品时必须谨慎。
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引用次数: 0
Comparison of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy Labsan Tricell-1000 和 Dirui FUS-2000 自动尿液分析仪与人工显微镜的比较
IF 1.9 Q3 Health Professions Pub Date : 2024-03-01 DOI: 10.1016/j.plabm.2024.e00386
Sedat Abusoglu , Halil Guven , Busra Ecer , Ahmet Emre Yorulmaz , Abdullah Sivrikaya , Fatma Humeyra Yerlikaya Aydemir , Ali Unlu , Gulsum Abusoglu , Muhittin Abdulkadir Serdar

Objectives

Urinalysis is a first-line test for screening for urinary tract infection. Several devices performing strip and sediment analysis have been introduced. The aim of this study was to compare the performance of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy.

Methods

463 urine samples were analyzed. Digital image processing and particle recognition automatically display the cells in a flowing sheath fluid mixed monolayer urine sample, take the pictures of particles via digital camera, analyse these pics with a particle recognition software, transfer images of the formed elements to the screen and allow well-trained personnel to select, reclassify or remove them. Manual microscopy was used for comparison.

Results

Agreement between Tricell-100 and manual microscopy was very good for RBC (ϰ = 0.80), and WBC (ϰ = 0.83); good for CaOx (ϰ = 0.69), SEC (ϰ = 0.80), YLC (ϰ = 0.72), HC (0.69) and LC (ϰ = 0.64); moderate for BAC (ϰ = 0.51), APC (ϰ = 0.43) and MT (ϰ = 0.55); fair for GC (ϰ = 0.39) and RTEC (ϰ = 0.32).

Conclusions

Labsan Trion TriCell-1000 demonstrated satisfactory performance and can be used in routine urinalysis. In the case of low counts of RBC, presence of yeast, crystal, casts or cell clumping in urine sediment, characterization of urine particles should be performed by manual microscopy.

目的尿液分析是筛查尿路感染的一线检测方法。目前已推出了几种可进行条带和沉淀物分析的设备。本研究旨在比较 Labsan Tricell-1000 和 Dirui FUS-2000 自动尿液分析仪与人工显微镜的性能。数字图像处理和颗粒识别自动显示流动的鞘液混合单层尿样中的细胞,通过数码相机拍摄颗粒照片,用颗粒识别软件分析这些照片,将形成的元素图像传输到屏幕上,并允许训练有素的人员选择、重新分类或删除它们。结果对于 RBC(ϰ = 0.80)和 WBC(ϰ = 0.83),Tricell-100 与手动显微镜的一致性非常好;对于 CaOx(ϰ = 0.69)、SEC(ϰ = 0.80)、YLC(ϰ = 0.72)、HC(0.69)和 LC(ϰ = 0.结论Labsan Trion TriCell-1000 的性能令人满意,可用于常规尿液分析。如果尿沉渣中的红细胞计数较低、存在酵母菌、晶体、铸型或细胞团块,则应通过人工显微镜对尿液颗粒进行定性。
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引用次数: 0
Added value of a connected glucose meter for glycorrhachia assessment 连接式血糖仪在糖尿评估中的附加值
IF 1.9 Q3 Health Professions Pub Date : 2024-03-01 DOI: 10.1016/j.plabm.2024.e00384
Laurent Blairon , Marie Tré-Hardy , Sophie Collignon , François Coenen , Ingrid Beukinga , Roberto Cupaiolo

Objectives

The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer.

Design and Methods

Intra-assay and inter-assay accuracies were calculated using residual clinical samples. Accuracies were measured by comparing the results obtained with the glucometer to those from the central laboratory on a large routine chemistry platform.

Results

The intra-assay coefficients of variation were between 6.1% and 6.2% for low values (18 mg/dL) and between 5.6% and 6.8% for high values (58 mg/dL). The inter-assay coefficients of variation were between 9.4% and 16.3% for the low values (18 mg/dL) and between 5.7% and 8.7% for the high values (pool; ±75 mg/dL). The regression equation by comparison to the central laboratory was y = 4.08 + 0.82 x, with a coefficient of determination (r2) of 0.95.

Conclusions

The measurement of glycorrhachia with a connected glucometer before the analysis in the central laboratory allows a rapid orientation in the deferential diagnosis of a meningitis of viral vs bacterial origin. The response time is fast (6 s) and requires only a small amount of fluid (1.2 μL), which is important in infants, especially since lumbar puncture is an integral part of the investigation of the origin of a fever in this population.

设计与方法使用残留的临床样本计算测定内和测定间的准确度。结果低值(18 毫克/分升)的测定结果的测定内变异系数为 6.1%至 6.2%,高值(58 毫克/分升)的测定结果的测定内变异系数为 5.6%至 6.8%。低值(18 毫克/分升)的测定间变异系数为 9.4% 至 16.3%,高值(池;±75 毫克/分升)的测定间变异系数为 5.7% 至 8.7%。结论在中心实验室进行分析之前,使用连接的血糖仪测量血糖,可以快速确定病毒性脑膜炎与细菌性脑膜炎的诊断方向。反应时间短(6 秒),只需少量液体(1.2 μL),这对婴儿非常重要,尤其是因为腰椎穿刺是调查婴儿发烧原因不可或缺的一部分。
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引用次数: 0
A comparison of chemiluminescent immunoassay and enzyme-linked immunosorbent assay for detecting phospholipase A2 receptor antibody in primary membranous nephropathy 化学发光免疫分析法与酶联免疫吸附法检测原发性膜性肾病磷脂酶 A2 受体抗体的比较
IF 1.9 Q3 Health Professions Pub Date : 2024-03-01 DOI: 10.1016/j.plabm.2024.e00385
Xiaotao Ma , Ruiting Wang , Linting Wei , Pengfei Liu , Lanmei Jing , Jinghua Wang , Wei Dong , Xuefei Tian , Rongguo Fu

Objective

The accurate detection of phospholipase A2 receptor (PLA2R) autoantibody is crucial in the diagnosis and monitoring of primary membranous nephropathy (pMN). While enzyme-linked immunosorbent assay (ELISA) is the commonly used detection method, its complexity and time-consuming nature pose challenges, especially for small sample sizes. Chemiluminescence immunoassay (CLIA) has emerged as a rapid alternative for clinical immunoassays. This study aims to compare the sensitivity, specificity, and precision of CLIA and ELISA in detecting PLA2R autoantibody.

Method

A total of 145 patients with biopsy-confirmed primary membranous nephropathy and 85 patients with non-membranous nephropathy were enrolled in this comparative study. CLIA and ELISA were employed to test all samples for the presence of PLA2R autoantibodies. Statistical analysis of sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) was performed using SPSS 26.0. The diagnostic value of ELISA and CLIA for pMN was analyzed using the ROC curve, and Correlation analysis was performed using Spearman.

Results

Serum levels of anti-PLA2R antibody in pMN group were significantly higher than those in nMN group(P < 0.05). The accuracy of CLIA for detecting anti-PLA2R antibody was 76.96%, while ELISA showed an accuracy of 74.78%. The sensitivity for CLIA was 64.83%, compared to 60% for ELISA. However, no statistically significant difference was observed between the two methods (P > 0.05). The overall qualitative agreement of anti-PLA2R detection was 93.35% (95% confidence interval[CI] 89.47–96.3). ROC curve analysis showed that AUC of anti-PLA2R antibody detected by ELISA and CLIA were 0.8737 (95% confidence interval [CI] 0.8270–0.9204), 0.8914 (95% confidence interval [CI]0.8495–0.9332), respectively. The Spearman correlation analysis revealed a significant correlation between them(P < 0.05). Notably, CLIA demonstrated a significant time-saving advantage, particularly when the sample size was less than 200, and especially when it was less than 20.

Conclusion

CLIA and ELISA showed similar accuracy and consistency in detecting anti-PLA2R antibody for primary membranous nephropathy. However, CLIA exhibited a significant advantage in terms of automation and time-saving compared to ELISA, particularly for smaller sample sizes. This finding suggests that CLIA has the potential to become a preferred and widely adopted test in the future.

目的准确检测磷脂酶 A2 受体(PLA2R)自身抗体对于诊断和监测原发性膜性肾病(pMN)至关重要。虽然酶联免疫吸附试验(ELISA)是常用的检测方法,但其复杂性和耗时性带来了挑战,尤其是对小样本量的检测。化学发光免疫测定(CLIA)已成为临床免疫测定的快速替代方法。本研究旨在比较 CLIA 和 ELISA 检测 PLA2R 自身抗体的灵敏度、特异性和精确度。采用 CLIA 和 ELISA 方法检测所有样本中是否存在 PLA2R 自身抗体。使用 SPSS 26.0 对敏感性、特异性、准确性、阳性预测值(PPV)和阴性预测值(NPV)进行了统计分析。结果 pMN 组血清中抗 PLA2R 抗体水平显著高于 nMN 组(P < 0.05)。CLIA 检测抗 PLA2R 抗体的准确率为 76.96%,而 ELISA 的准确率为 74.78%。CLIA 的灵敏度为 64.83%,而 ELISA 为 60%。不过,两种方法之间的差异没有统计学意义(P > 0.05)。抗 PLA2R 检测的总体定性一致率为 93.35%(95% 置信区间[CI] 89.47-96.3)。ROC曲线分析显示,ELISA和CLIA检测抗PLA2R抗体的AUC分别为0.8737(95%置信区间[CI]0.8270-0.9204)和0.8914(95%置信区间[CI]0.8495-0.9332)。斯皮尔曼相关分析表明,它们之间存在显著相关性(P < 0.05)。结论 CLIA 和 ELISA 在检测原发性膜性肾病的抗 PLA2R 抗体方面表现出相似的准确性和一致性。然而,与 ELISA 相比,CLIA 在自动化和节省时间方面具有明显优势,尤其是在样本量较小的情况下。这一发现表明,CLIA 有可能成为未来首选并被广泛采用的检测方法。
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Practical Laboratory Medicine
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