Pub Date : 2024-05-01DOI: 10.1016/j.plabm.2024.e00404
Xin-Yu Wang , Xiangzhi Liu , Chengliang Zhen , Nannan Tian , Haina Ma , Menghan Wang , Li Wang
This study aims to investigate the correlation between plasma fat-soluble vitamin levels and blood lipid in elderly patients with coronary heart disease (CHD). A total of 120 participants were enrolled, including 60 CHD patients and 60 controls without CHD. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify plasma levels of vitamins A, D3, E, and K. Data analysis was conducted using the statistical analysis system module of MetaboAnalyst 5.0. The CHD group showed significantly higher levels of plasma total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) but not high-density lipoprotein cholesterol (HDL-C) compared to controls. The CHD group exhibited significantly higher plasma levels of VA and VE, positively correlating with TC, TG, and LDL-C. After adjusted by TG levels, the CHD group had significantly lower plasma levels of VA and VE, negatively correlating with TC, TG, and LDL-C. The CHD group also had significantly lower concentrations of VD3, independent of TG modification, compared to controls. VD3 negatively correlated with TC, TG, and LDL-C. Elderly individuals with CHD display abnormal blood lipid metabolism, and fat-soluble vitamins adjusted by TG levels can more accurately and timely response to implicit fat-soluble vitamins deficiency in CHD patients.
本研究旨在探讨老年冠心病(CHD)患者血浆脂溶性维生素水平与血脂之间的相关性。研究共招募了 120 名参与者,包括 60 名冠心病患者和 60 名非冠心病对照组。采用液相色谱-串联质谱法(LC-MS/MS)定量检测血浆中维生素A、D3、E和K的含量。与对照组相比,CHD组的血浆总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)水平明显较高,但高密度脂蛋白胆固醇(HDL-C)水平并不高。心脏病组的血浆 VA 和 VE 水平明显较高,与 TC、TG 和 LDL-C 呈正相关。根据 TG 水平进行调整后,CHD 组的血浆 VA 和 VE 水平明显较低,与 TC、TG 和 LDL-C 呈负相关。与对照组相比,冠心病组的 VD3 浓度也明显较低,与 TG 的变化无关。VD3 与总胆固醇、总胆固醇和低密度脂蛋白胆固醇呈负相关。患有冠心病的老年人血脂代谢异常,根据 TG 水平调整脂溶性维生素可以更准确、更及时地应对冠心病患者隐性脂溶性维生素缺乏的问题。
{"title":"Correction of plasma fat-soluble vitamin levels by blood lipids in elderly patients with coronary heart disease","authors":"Xin-Yu Wang , Xiangzhi Liu , Chengliang Zhen , Nannan Tian , Haina Ma , Menghan Wang , Li Wang","doi":"10.1016/j.plabm.2024.e00404","DOIUrl":"10.1016/j.plabm.2024.e00404","url":null,"abstract":"<div><p>This study aims to investigate the correlation between plasma fat-soluble vitamin levels and blood lipid in elderly patients with coronary heart disease (CHD). A total of 120 participants were enrolled, including 60 CHD patients and 60 controls without CHD. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify plasma levels of vitamins A, D<sub>3</sub>, E, and K. Data analysis was conducted using the statistical analysis system module of MetaboAnalyst 5.0. The CHD group showed significantly higher levels of plasma total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) but not high-density lipoprotein cholesterol (HDL-C) compared to controls. The CHD group exhibited significantly higher plasma levels of VA and VE, positively correlating with TC, TG, and LDL-C. After adjusted by TG levels, the CHD group had significantly lower plasma levels of VA and VE, negatively correlating with TC, TG, and LDL-C. The CHD group also had significantly lower concentrations of VD<sub>3</sub>, independent of TG modification, compared to controls. VD<sub>3</sub> negatively correlated with TC, TG, and LDL-C. Elderly individuals with CHD display abnormal blood lipid metabolism, and fat-soluble vitamins adjusted by TG levels can more accurately and timely response to implicit fat-soluble vitamins deficiency in CHD patients.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000507/pdfft?md5=89f9eecd2162905a759d3140a4daac47&pid=1-s2.0-S2352551724000507-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141140924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1016/j.plabm.2024.e00414
Yuan Yu , Xiaoyun Zhang , Kai Lin
Background
Most glycated hemoglobin A1c (HbA1c) analytical reagents used were obtained from the analyzer's manufacturer. However, clinical laboratories need more choices for HbA1c analytical reagents to overcome the limitations of dedicated reagents for special analyzers. We developed new mobile phase buffers as HbA1c diagnostic reagents and evaluated their analytical performance for the HbA1c assay.
Methods
Different mobile phase buffers used as HbA1c diagnostic reagents were prepared using different concentrations of sodium salts. According to the Clinical and Laboratory Standards Institute (CLSI) recommendation guidelines, the analytical performances of the newly developed mobile phase buffers were evaluated on an ARKRAY HA-8160 Analyzer. Both quality controls and clinical blood samples were used in these experiments. To assess the quality of the newly developed mobile phase buffers, precision, accuracy, linearity, carryover, interference, bias, correlation with commercial reagents, and stability were analyzed.
Results
The CVs of intra-assay precision and interassay precision of quality control and clinical.
There were fewer than 1.00 % blood sample assays using the newly developed mobile phase buffer. The RDs of accuracy were less than 1.00 %. Linearity: R2 = 0.9998 in the concentration range of 4.40%–17.30 %. Carryover: 0.00 %. Reagent comparison revealed that the Pearson regression equation was Y = 0.9884x+0.05692 (R2 = 0.9977), and the Bland-Altman mean difference was −0.02650 % (CI: −0.2121 %–0.1591 %) between the two analytical reagents. Stability was also acceptable within 12 months. This mobile phase buffer showed good anti-interference ability.
Conclusion
The newly developed mobile phase buffers demonstrated good analytical performance and were suitable for clinical HbA1c assays on an ARKRAY HA-8160 Analyzer.
{"title":"Analytical performance evaluation of hemoglobin A1c on an ARKRAY HA-8160 analyzer with newly-developed mobile phase buffer","authors":"Yuan Yu , Xiaoyun Zhang , Kai Lin","doi":"10.1016/j.plabm.2024.e00414","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00414","url":null,"abstract":"<div><h3>Background</h3><p>Most glycated hemoglobin A1c (HbA1c) analytical reagents used were obtained from the analyzer's manufacturer. However, clinical laboratories need more choices for HbA1c analytical reagents to overcome the limitations of dedicated reagents for special analyzers. We developed new mobile phase buffers as HbA1c diagnostic reagents and evaluated their analytical performance for the HbA1c assay.</p></div><div><h3>Methods</h3><p>Different mobile phase buffers used as HbA1c diagnostic reagents were prepared using different concentrations of sodium salts. According to the Clinical and Laboratory Standards Institute (CLSI) recommendation guidelines, the analytical performances of the newly developed mobile phase buffers were evaluated on an ARKRAY HA-8160 Analyzer. Both quality controls and clinical blood samples were used in these experiments. To assess the quality of the newly developed mobile phase buffers, precision, accuracy, linearity, carryover, interference, bias, correlation with commercial reagents, and stability were analyzed.</p></div><div><h3>Results</h3><p>The <em>CV</em>s of intra-assay precision and interassay precision of quality control and clinical.</p><p>There were fewer than 1.00 % blood sample assays using the newly developed mobile phase buffer. The <em>RDs</em> of accuracy were less than 1.00 %. Linearity: R<sup>2</sup> = 0.9998 in the concentration range of 4.40%–17.30 %. Carryover: 0.00 %. Reagent comparison revealed that the Pearson regression equation was Y = 0.9884x+0.05692 (R<sup>2</sup> = 0.9977), and the Bland-Altman mean difference was −0.02650 % (CI: −0.2121 %–0.1591 %) between the two analytical reagents. Stability was also acceptable within 12 months. This mobile phase buffer showed good anti-interference ability.</p></div><div><h3>Conclusion</h3><p>The newly developed mobile phase buffers demonstrated good analytical performance and were suitable for clinical HbA1c assays on an ARKRAY HA-8160 Analyzer.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S235255172400060X/pdfft?md5=f33377b107c40be9e47def3bbf412eb8&pid=1-s2.0-S235255172400060X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1016/j.plabm.2024.e00411
Liu Dong , Xiaoqing Wang , Qianqian Xu, Ruoshui Cao, Xuan Deng, Jian Chen, Haoqin Jiang
Background
Pandy's test is used to assess the globulin level in cerebrospinal fluid (CSF). As a semi-quantitative manual method, the practicality and clinical value of Pandy's test has been challenged.
Objective
We tend to summarize the relationship between CSF total protein (CSF-TP) quantification and Pandy's results, providing a formula to estimate Pandy's results merely by CSF-TP value.
Methods
This retrospective study involved 1090 cases hospitalized in Huashan Hospital during 1/1/2023 to 20/4/2023. All samples were divided into six group based on their Pandy's results. Their corresponding CSF-TP quantitative results were subsequently analyzed and summarized. Another 364 patients were also gathered for verification.
Results
The turbidity of samples won't affect examiners'ocular inspection and interpretation of Pandy's tests in positive groups. The results of Pandy's tests can be deduced based on CSF-TP quantitative results according to following rules: CSF-TP quantitative results 0–614 mg/L for Pandy negative (−), 615–1322 mg/L for extremely weak positive (±), 1323–2953 mg/L for weak positive (1+), 2954–6561 mg/L for medium positive results (2+), 6562–13007 mg/L for strong positive results (3+) and CSF-TP results >13007 for strongest positive (4+). The quantitative range above was experimentally verified as effective and correct by calculating the agreement rate through another 364 samples and the R ratio of each Pandy group was greater than 90 %.
Conclusion
There is an excellent correlation between CSF-TP and Pandy's test. Therefore, CSF-TP quantification test through PROT Slides can be used to infer the results of Pandy's test to accelerate the abolish of this traditional manual test.
{"title":"An alternative method for inferring Pandy's test using cerebrospinal fluid total protein","authors":"Liu Dong , Xiaoqing Wang , Qianqian Xu, Ruoshui Cao, Xuan Deng, Jian Chen, Haoqin Jiang","doi":"10.1016/j.plabm.2024.e00411","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00411","url":null,"abstract":"<div><h3>Background</h3><p>Pandy's test is used to assess the globulin level in cerebrospinal fluid (CSF). As a semi-quantitative manual method, the practicality and clinical value of Pandy's test has been challenged.</p></div><div><h3>Objective</h3><p>We tend to summarize the relationship between CSF total protein (CSF-TP) quantification and Pandy's results, providing a formula to estimate Pandy's results merely by CSF-TP value.</p></div><div><h3>Methods</h3><p>This retrospective study involved 1090 cases hospitalized in Huashan Hospital during 1/1/2023 to 20/4/2023. All samples were divided into six group based on their Pandy's results. Their corresponding CSF-TP quantitative results were subsequently analyzed and summarized. Another 364 patients were also gathered for verification.</p></div><div><h3>Results</h3><p>The turbidity of samples won't affect examiners'ocular inspection and interpretation of Pandy's tests in positive groups. The results of Pandy's tests can be deduced based on CSF-TP quantitative results according to following rules: CSF-TP quantitative results 0–614 mg/L for Pandy negative (−), 615–1322 mg/L for extremely weak positive (±), 1323–2953 mg/L for weak positive (1+), 2954–6561 mg/L for medium positive results (2+), 6562–13007 mg/L for strong positive results (3+) and CSF-TP results >13007 for strongest positive (4+). The quantitative range above was experimentally verified as effective and correct by calculating the agreement rate through another 364 samples and the R ratio of each Pandy group was greater than 90 %.</p></div><div><h3>Conclusion</h3><p>There is an excellent correlation between CSF-TP and Pandy's test. Therefore, CSF-TP quantification test through PROT Slides can be used to infer the results of Pandy's test to accelerate the abolish of this traditional manual test.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S235255172400057X/pdfft?md5=b5b628c6d5255304cd0b92e6160d6cd3&pid=1-s2.0-S235255172400057X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141239037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-18DOI: 10.1016/j.plabm.2024.e00395
Abidah Mobarak , Subashini C. Thambiah , Ana Daliela Masiman , Intan Nureslyna Samsudin , Yin Ye Lai
Acquired methemoglobinemia, predominantly due to oxidizing medications occurs when heme iron in hemoglobin is oxidized from ferrous to ferric ion and binds oxygen irreversibly leading to functional anemia, cyanosis, and tissue hypoxia. We report a case of a 60-year-old man with multiple comorbidities who was diagnosed with coronavirus disease 2019 (COVID-19) and developed methemoglobinemia after consumption of prescribed supplements. He presented with dyspnea and cyanosis. An oxygen saturation gap with characteristic chocolate-brown arterial blood indicated methemoglobinemia. Outsourced methemoglobin (MetHb) was increased at 9.0%. Despite aggressive intervention, he succumbed to his illness. In this case, we discuss the pathophysiology of why some individuals, especially the elderly with COVID-19 are more susceptible to develop methemoglobinemia after possibly being exposed to oxidizing agents. Laboratory methods for assessing oxygen saturation, including pulse oximetry, arterial blood gas and co-oximetry are examined in relation to this case. The importance of considering a diagnosis of methemoglobinemia based on clinical and biochemical findings although MetHb assay or co-oximetry are not readily available is also emphasized.
{"title":"Refractory hypoxia and saturation gap in a COVID-19 patient","authors":"Abidah Mobarak , Subashini C. Thambiah , Ana Daliela Masiman , Intan Nureslyna Samsudin , Yin Ye Lai","doi":"10.1016/j.plabm.2024.e00395","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00395","url":null,"abstract":"<div><p>Acquired methemoglobinemia, predominantly due to oxidizing medications occurs when heme iron in hemoglobin is oxidized from ferrous to ferric ion and binds oxygen irreversibly leading to functional anemia, cyanosis, and tissue hypoxia. We report a case of a 60-year-old man with multiple comorbidities who was diagnosed with coronavirus disease 2019 (COVID-19) and developed methemoglobinemia after consumption of prescribed supplements. He presented with dyspnea and cyanosis. An oxygen saturation gap with characteristic chocolate-brown arterial blood indicated methemoglobinemia. Outsourced methemoglobin (MetHb) was increased at 9.0%. Despite aggressive intervention, he succumbed to his illness. In this case, we discuss the pathophysiology of why some individuals, especially the elderly with COVID-19 are more susceptible to develop methemoglobinemia after possibly being exposed to oxidizing agents. Laboratory methods for assessing oxygen saturation, including pulse oximetry, arterial blood gas and co-oximetry are examined in relation to this case. The importance of considering a diagnosis of methemoglobinemia based on clinical and biochemical findings although MetHb assay or co-oximetry are not readily available is also emphasized.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000416/pdfft?md5=f4925cc54af639949a5b0292b7eee6dd&pid=1-s2.0-S2352551724000416-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140639349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-16DOI: 10.1016/j.plabm.2024.e00394
Guanbin Zhang , Xiaoying Lin , Wenkun Mu , Jun Luo , Yiyuan Xu , Chicheng Song , Jiang Li
Objectives
The clamshell isothermal nucleic acid amplification analyzer RTisochip-S, a next-generation instrument featuring improved structural design, enhanced functional integration, reduced cost, and increased portability, was assessed for its suitability in clinical respiratory pathogens detection.
Methods
The certificated detection kit for lower respiratory tract bacteria (LRTB-kit) was applied to evaluate the performance of RTisochip-S via sensitivity, specificity, and repeatability analysis. The clinical specimens, including 51 sputum specimens and 10 bronchoalveolar lavage fluid specimens, were simultaneously detected on both RTisochip-S and a certificated reference instrument (RTisochip-A) to assess the consistency.
Results
The results indicated that RTisochip-S fulfills the sensitivity, specificity, and repeatability requirements of the LRTB-Kit, and the results of clinical specimens on the two instruments were consistent.
Conclusions
RTisochip-S is satisfying the clinical detection of respiratory pathogens while enhancing portability and compactness, making it more well-suited for point-of-care testing (POCT) applications.
{"title":"Application of a clamshell isothermal nucleic acid amplification analyzer in the detection of lower respiratory tract bacteria","authors":"Guanbin Zhang , Xiaoying Lin , Wenkun Mu , Jun Luo , Yiyuan Xu , Chicheng Song , Jiang Li","doi":"10.1016/j.plabm.2024.e00394","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00394","url":null,"abstract":"<div><h3>Objectives</h3><p>The clamshell isothermal nucleic acid amplification analyzer RTisochip-S, a next-generation instrument featuring improved structural design, enhanced functional integration, reduced cost, and increased portability, was assessed for its suitability in clinical respiratory pathogens detection.</p></div><div><h3>Methods</h3><p>The certificated detection kit for lower respiratory tract bacteria (LRTB-kit) was applied to evaluate the performance of RTisochip-S via sensitivity, specificity, and repeatability analysis. The clinical specimens, including 51 sputum specimens and 10 bronchoalveolar lavage fluid specimens, were simultaneously detected on both RTisochip-S and a certificated reference instrument (RTisochip-A) to assess the consistency.</p></div><div><h3>Results</h3><p>The results indicated that RTisochip-S fulfills the sensitivity, specificity, and repeatability requirements of the LRTB-Kit, and the results of clinical specimens on the two instruments were consistent.</p></div><div><h3>Conclusions</h3><p>RTisochip-S is satisfying the clinical detection of respiratory pathogens while enhancing portability and compactness, making it more well-suited for point-of-care testing (POCT) applications.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000404/pdfft?md5=cf27d7a272776a666a30c260483b2917&pid=1-s2.0-S2352551724000404-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140618087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-09DOI: 10.1016/j.plabm.2024.e00393
Anna Lee , Sooah Jang , Sanghoo Lee , Hyun-Kyung Park , In-Young Kim , Ryunsup Ahn , Jeong-Ho Seok , Kyoung-Ryul Lee
Objectives
Salivary cortisol reflects the biologically active form of serum cortisol, offering a noninvasive evaluation method for the diurnal rhythm of the hypothalamic-pituitary-adrenal (HPA) axis. While liquid chromatography-tandem mass spectrometry (LC-MS/MS) is known for its specificity, immunoassays (IA) are commonly used because of their simplicity. This study aimed to assess the performance of salivary cortisol measurement using both IA and LC-MS/MS in comparison to serum-free cortisol measurement.
Methods
Assay results for 188 saliva and 94 serum samples from 47 participants were analyzed. Salivary samples collected at different time points were analyzed using IA and LC-MS/MS. Serum samples were analyzed for cortisol, cortisol-binding globulin, and free cortisol. The statistical analyses included correlations and method comparisons.
Results
The diurnal salivary cortisol profiles exhibited a comparable circadian rhythm pattern; however, the concentrations measured using IA were consistently higher than those measured using LC-MS/MS. The correlation analysis revealed robust associations among salivary cortisol (IA), salivary cortisol (LC-MS/MS), and serum-free cortisol levels (LC-MS/MS). However, the method comparison revealed a systematic bias between IA and LC-MS/MS in salivary cortisol measurement.
Conclusions
This study contributes to the ongoing debate on assay techniques by affirming the suitability of IA and LC-MS/MS for salivary cortisol measurement to assess dynamic changes in HPA axis activity. The identified systematic bias emphasizes the importance of selecting methods based on specific research or clinical requirements.
目的涎皮质醇反映了血清皮质醇的生物活性形式,为下丘脑-垂体-肾上腺(HPA)轴的昼夜节律提供了一种无创评估方法。液相色谱-串联质谱法(LC-MS/MS)以其特异性著称,而免疫测定法(IA)则因其简便性而常用。本研究旨在评估使用免疫测定法和 LC-MS/MS 测量唾液皮质醇的性能,并与无血清皮质醇测量进行比较。采用 IA 和 LC-MS/MS 分析了在不同时间点采集的唾液样本。对血清样本进行了皮质醇、皮质醇结合球蛋白和游离皮质醇分析。结果昼夜唾液皮质醇曲线表现出相似的昼夜节律模式;但是,用 IA 测得的浓度始终高于用 LC-MS/MS 测得的浓度。相关性分析表明,唾液皮质醇(IA)、唾液皮质醇(LC-MS/MS)和无血清皮质醇水平(LC-MS/MS)之间存在很强的相关性。结论 该研究肯定了唾液皮质醇测量中 IA 和 LC-MS/MS 在评估 HPA 轴活动动态变化方面的适用性,从而为目前关于检测技术的争论做出了贡献。发现的系统性偏差强调了根据特定研究或临床要求选择方法的重要性。
{"title":"Comparative analysis of salivary cortisol measurements using different assay methods in relation to serum-free cortisol measurement","authors":"Anna Lee , Sooah Jang , Sanghoo Lee , Hyun-Kyung Park , In-Young Kim , Ryunsup Ahn , Jeong-Ho Seok , Kyoung-Ryul Lee","doi":"10.1016/j.plabm.2024.e00393","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00393","url":null,"abstract":"<div><h3>Objectives</h3><p>Salivary cortisol reflects the biologically active form of serum cortisol, offering a noninvasive evaluation method for the diurnal rhythm of the hypothalamic-pituitary-adrenal (HPA) axis. While liquid chromatography-tandem mass spectrometry (LC-MS/MS) is known for its specificity, immunoassays (IA) are commonly used because of their simplicity. This study aimed to assess the performance of salivary cortisol measurement using both IA and LC-MS/MS in comparison to serum-free cortisol measurement.</p></div><div><h3>Methods</h3><p>Assay results for 188 saliva and 94 serum samples from 47 participants were analyzed. Salivary samples collected at different time points were analyzed using IA and LC-MS/MS. Serum samples were analyzed for cortisol, cortisol-binding globulin, and free cortisol. The statistical analyses included correlations and method comparisons.</p></div><div><h3>Results</h3><p>The diurnal salivary cortisol profiles exhibited a comparable circadian rhythm pattern; however, the concentrations measured using IA were consistently higher than those measured using LC-MS/MS. The correlation analysis revealed robust associations among salivary cortisol (IA), salivary cortisol (LC-MS/MS), and serum-free cortisol levels (LC-MS/MS). However, the method comparison revealed a systematic bias between IA and LC-MS/MS in salivary cortisol measurement.</p></div><div><h3>Conclusions</h3><p>This study contributes to the ongoing debate on assay techniques by affirming the suitability of IA and LC-MS/MS for salivary cortisol measurement to assess dynamic changes in HPA axis activity. The identified systematic bias emphasizes the importance of selecting methods based on specific research or clinical requirements.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000398/pdfft?md5=89279cbc1bebd280b01274033e305a55&pid=1-s2.0-S2352551724000398-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140549343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.1016/j.plabm.2024.e00383
Zejian Zhang , Xiangyi Cui , Nan Zhou , Lisi Zhu , Yuxiang Zhi , Shuyang Zhang
Background and aims
Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.
Materials and methods
We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.
Results
Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits.
Conclusion
Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.
{"title":"Influence of plasma collection tubes on N-glycome in human blood samples","authors":"Zejian Zhang , Xiangyi Cui , Nan Zhou , Lisi Zhu , Yuxiang Zhi , Shuyang Zhang","doi":"10.1016/j.plabm.2024.e00383","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00383","url":null,"abstract":"<div><h3>Background and aims</h3><p>Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.</p></div><div><h3>Materials and methods</h3><p>We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.</p></div><div><h3>Results</h3><p>Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits, and between EDTA and citrate plasma in 62 out of 82 traits. These differences encompassed various N-glycan features, including glycan type, sialylation, galactosylation, fucosylation, and bisection. Trends in N-glycan variations in citrate and heparin plasma were largely consistent compared to EDTA plasma. In correlation analysis (EDTA vs. heparin; EDTA vs. citrate), Pearson's correlation coefficients were consistently higher than 0.7 for the majority of N-glycan traits.</p></div><div><h3>Conclusion</h3><p>Sample matrix variations impact plasma N-glycome measurements. Caution is crucial when comparing samples from different plasma collection tubes in glycomics projects.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000295/pdfft?md5=2f804ffd65e96a5c5246b7e8c695a672&pid=1-s2.0-S2352551724000295-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.1016/j.plabm.2024.e00386
Sedat Abusoglu , Halil Guven , Busra Ecer , Ahmet Emre Yorulmaz , Abdullah Sivrikaya , Fatma Humeyra Yerlikaya Aydemir , Ali Unlu , Gulsum Abusoglu , Muhittin Abdulkadir Serdar
Objectives
Urinalysis is a first-line test for screening for urinary tract infection. Several devices performing strip and sediment analysis have been introduced. The aim of this study was to compare the performance of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy.
Methods
463 urine samples were analyzed. Digital image processing and particle recognition automatically display the cells in a flowing sheath fluid mixed monolayer urine sample, take the pictures of particles via digital camera, analyse these pics with a particle recognition software, transfer images of the formed elements to the screen and allow well-trained personnel to select, reclassify or remove them. Manual microscopy was used for comparison.
Results
Agreement between Tricell-100 and manual microscopy was very good for RBC (ϰ = 0.80), and WBC (ϰ = 0.83); good for CaOx (ϰ = 0.69), SEC (ϰ = 0.80), YLC (ϰ = 0.72), HC (0.69) and LC (ϰ = 0.64); moderate for BAC (ϰ = 0.51), APC (ϰ = 0.43) and MT (ϰ = 0.55); fair for GC (ϰ = 0.39) and RTEC (ϰ = 0.32).
Conclusions
Labsan Trion TriCell-1000 demonstrated satisfactory performance and can be used in routine urinalysis. In the case of low counts of RBC, presence of yeast, crystal, casts or cell clumping in urine sediment, characterization of urine particles should be performed by manual microscopy.
{"title":"Comparison of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy","authors":"Sedat Abusoglu , Halil Guven , Busra Ecer , Ahmet Emre Yorulmaz , Abdullah Sivrikaya , Fatma Humeyra Yerlikaya Aydemir , Ali Unlu , Gulsum Abusoglu , Muhittin Abdulkadir Serdar","doi":"10.1016/j.plabm.2024.e00386","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00386","url":null,"abstract":"<div><h3>Objectives</h3><p>Urinalysis is a first-line test for screening for urinary tract infection. Several devices performing strip and sediment analysis have been introduced. The aim of this study was to compare the performance of Labsan Tricell-1000 and Dirui FUS-2000 automated urine analyzers with manual microscopy.</p></div><div><h3>Methods</h3><p>463 urine samples were analyzed. Digital image processing and particle recognition automatically display the cells in a flowing sheath fluid mixed monolayer urine sample, take the pictures of particles via digital camera, analyse these pics with a particle recognition software, transfer images of the formed elements to the screen and allow well-trained personnel to select, reclassify or remove them. Manual microscopy was used for comparison.</p></div><div><h3>Results</h3><p>Agreement between Tricell-100 and manual microscopy was very good for RBC (ϰ = 0.80), and WBC (ϰ = 0.83); good for CaOx (ϰ = 0.69), SEC (ϰ = 0.80), YLC (ϰ = 0.72), HC (0.69) and LC (ϰ = 0.64); moderate for BAC (ϰ = 0.51), APC (ϰ = 0.43) and MT (ϰ = 0.55); fair for GC (ϰ = 0.39) and RTEC (ϰ = 0.32).</p></div><div><h3>Conclusions</h3><p>Labsan Trion TriCell-1000 demonstrated satisfactory performance and can be used in routine urinalysis. In the case of low counts of RBC, presence of yeast, crystal, casts or cell clumping in urine sediment, characterization of urine particles should be performed by manual microscopy.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000325/pdfft?md5=7f05a63231e96c752eb99bf724ad7d26&pid=1-s2.0-S2352551724000325-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140015837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.1016/j.plabm.2024.e00384
Laurent Blairon , Marie Tré-Hardy , Sophie Collignon , François Coenen , Ingrid Beukinga , Roberto Cupaiolo
Objectives
The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer.
Design and Methods
Intra-assay and inter-assay accuracies were calculated using residual clinical samples. Accuracies were measured by comparing the results obtained with the glucometer to those from the central laboratory on a large routine chemistry platform.
Results
The intra-assay coefficients of variation were between 6.1% and 6.2% for low values (18 mg/dL) and between 5.6% and 6.8% for high values (58 mg/dL). The inter-assay coefficients of variation were between 9.4% and 16.3% for the low values (18 mg/dL) and between 5.7% and 8.7% for the high values (pool; ±75 mg/dL). The regression equation by comparison to the central laboratory was y = 4.08 + 0.82 x, with a coefficient of determination (r2) of 0.95.
Conclusions
The measurement of glycorrhachia with a connected glucometer before the analysis in the central laboratory allows a rapid orientation in the deferential diagnosis of a meningitis of viral vs bacterial origin. The response time is fast (6 s) and requires only a small amount of fluid (1.2 μL), which is important in infants, especially since lumbar puncture is an integral part of the investigation of the origin of a fever in this population.
{"title":"Added value of a connected glucose meter for glycorrhachia assessment","authors":"Laurent Blairon , Marie Tré-Hardy , Sophie Collignon , François Coenen , Ingrid Beukinga , Roberto Cupaiolo","doi":"10.1016/j.plabm.2024.e00384","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00384","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to demonstrate the performance and added value of rapid glucose determination in cerebrospinal fluid using a connected glucometer.</p></div><div><h3>Design and Methods</h3><p>Intra-assay and inter-assay accuracies were calculated using residual clinical samples. Accuracies were measured by comparing the results obtained with the glucometer to those from the central laboratory on a large routine chemistry platform.</p></div><div><h3>Results</h3><p>The intra-assay coefficients of variation were between 6.1% and 6.2% for low values (18 mg/dL) and between 5.6% and 6.8% for high values (58 mg/dL). The inter-assay coefficients of variation were between 9.4% and 16.3% for the low values (18 mg/dL) and between 5.7% and 8.7% for the high values (pool; ±75 mg/dL). The regression equation by comparison to the central laboratory was y = 4.08 + 0.82 x, with a coefficient of determination (r<sup>2</sup>) of 0.95.</p></div><div><h3>Conclusions</h3><p>The measurement of glycorrhachia with a connected glucometer before the analysis in the central laboratory allows a rapid orientation in the deferential diagnosis of a meningitis of viral vs bacterial origin. The response time is fast (6 s) and requires only a small amount of fluid (1.2 μL), which is important in infants, especially since lumbar puncture is an integral part of the investigation of the origin of a fever in this population.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000301/pdfft?md5=832c69b64e8568ee7d0ec1e53b2808dd&pid=1-s2.0-S2352551724000301-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140031400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.1016/j.plabm.2024.e00385
Xiaotao Ma , Ruiting Wang , Linting Wei , Pengfei Liu , Lanmei Jing , Jinghua Wang , Wei Dong , Xuefei Tian , Rongguo Fu
Objective
The accurate detection of phospholipase A2 receptor (PLA2R) autoantibody is crucial in the diagnosis and monitoring of primary membranous nephropathy (pMN). While enzyme-linked immunosorbent assay (ELISA) is the commonly used detection method, its complexity and time-consuming nature pose challenges, especially for small sample sizes. Chemiluminescence immunoassay (CLIA) has emerged as a rapid alternative for clinical immunoassays. This study aims to compare the sensitivity, specificity, and precision of CLIA and ELISA in detecting PLA2R autoantibody.
Method
A total of 145 patients with biopsy-confirmed primary membranous nephropathy and 85 patients with non-membranous nephropathy were enrolled in this comparative study. CLIA and ELISA were employed to test all samples for the presence of PLA2R autoantibodies. Statistical analysis of sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) was performed using SPSS 26.0. The diagnostic value of ELISA and CLIA for pMN was analyzed using the ROC curve, and Correlation analysis was performed using Spearman.
Results
Serum levels of anti-PLA2R antibody in pMN group were significantly higher than those in nMN group(P < 0.05). The accuracy of CLIA for detecting anti-PLA2R antibody was 76.96%, while ELISA showed an accuracy of 74.78%. The sensitivity for CLIA was 64.83%, compared to 60% for ELISA. However, no statistically significant difference was observed between the two methods (P > 0.05). The overall qualitative agreement of anti-PLA2R detection was 93.35% (95% confidence interval[CI] 89.47–96.3). ROC curve analysis showed that AUC of anti-PLA2R antibody detected by ELISA and CLIA were 0.8737 (95% confidence interval [CI] 0.8270–0.9204), 0.8914 (95% confidence interval [CI]0.8495–0.9332), respectively. The Spearman correlation analysis revealed a significant correlation between them(P < 0.05). Notably, CLIA demonstrated a significant time-saving advantage, particularly when the sample size was less than 200, and especially when it was less than 20.
Conclusion
CLIA and ELISA showed similar accuracy and consistency in detecting anti-PLA2R antibody for primary membranous nephropathy. However, CLIA exhibited a significant advantage in terms of automation and time-saving compared to ELISA, particularly for smaller sample sizes. This finding suggests that CLIA has the potential to become a preferred and widely adopted test in the future.
{"title":"A comparison of chemiluminescent immunoassay and enzyme-linked immunosorbent assay for detecting phospholipase A2 receptor antibody in primary membranous nephropathy","authors":"Xiaotao Ma , Ruiting Wang , Linting Wei , Pengfei Liu , Lanmei Jing , Jinghua Wang , Wei Dong , Xuefei Tian , Rongguo Fu","doi":"10.1016/j.plabm.2024.e00385","DOIUrl":"https://doi.org/10.1016/j.plabm.2024.e00385","url":null,"abstract":"<div><h3>Objective</h3><p>The accurate detection of phospholipase A2 receptor (PLA2R) autoantibody is crucial in the diagnosis and monitoring of primary membranous nephropathy (pMN). While enzyme-linked immunosorbent assay (ELISA) is the commonly used detection method, its complexity and time-consuming nature pose challenges, especially for small sample sizes. Chemiluminescence immunoassay (CLIA) has emerged as a rapid alternative for clinical immunoassays. This study aims to compare the sensitivity, specificity, and precision of CLIA and ELISA in detecting PLA2R autoantibody.</p></div><div><h3>Method</h3><p>A total of 145 patients with biopsy-confirmed primary membranous nephropathy and 85 patients with non-membranous nephropathy were enrolled in this comparative study. CLIA and ELISA were employed to test all samples for the presence of PLA2R autoantibodies. Statistical analysis of sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) was performed using SPSS 26.0. The diagnostic value of ELISA and CLIA for pMN was analyzed using the ROC curve, and Correlation analysis was performed using Spearman.</p></div><div><h3>Results</h3><p>Serum levels of anti-PLA2R antibody in pMN group were significantly higher than those in nMN group(P < 0.05). The accuracy of CLIA for detecting anti-PLA2R antibody was 76.96%, while ELISA showed an accuracy of 74.78%. The sensitivity for CLIA was 64.83%, compared to 60% for ELISA. However, no statistically significant difference was observed between the two methods (<em>P</em> > 0.05). The overall qualitative agreement of anti-PLA2R detection was 93.35% (95% confidence interval[CI] 89.47–96.3). ROC curve analysis showed that AUC of anti-PLA2R antibody detected by ELISA and CLIA were 0.8737 (95% confidence interval [CI] 0.8270–0.9204), 0.8914 (95% confidence interval [CI]0.8495–0.9332), respectively. The Spearman correlation analysis revealed a significant correlation between them(<em>P</em> < 0.05). Notably, CLIA demonstrated a significant time-saving advantage, particularly when the sample size was less than 200, and especially when it was less than 20.</p></div><div><h3>Conclusion</h3><p>CLIA and ELISA showed similar accuracy and consistency in detecting anti-PLA2R antibody for primary membranous nephropathy. However, CLIA exhibited a significant advantage in terms of automation and time-saving compared to ELISA, particularly for smaller sample sizes. This finding suggests that CLIA has the potential to become a preferred and widely adopted test in the future.</p></div>","PeriodicalId":20421,"journal":{"name":"Practical Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352551724000313/pdfft?md5=77ed71aee7e179b45c671f2edfe84687&pid=1-s2.0-S2352551724000313-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}