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A robust procedure for P-wave detection and segmentation in high resolution 12-lead ECG 高分辨率12导联心电图p波检测和分割的鲁棒程序
O. Escalona, L. Gavidia, G. Wright
Reliable detection and measurement of the P-wave duration and position in high resolution 12-lead ECG recordings, was automated by means of a computer procedure which involved noise reduction by means of signal averaging, and bi-directional filtering of the P-wave at 25 Hz and the QRS complex at 40 Hz Then, algorithms for P-onset, P-offset and QRS-onset detection, allowed the estimation of P-wave duration and PQ interval, This computer procedure was clinically tested with 52 volunteers. The results indicated that, although reliable P-wave detection was possible in at least three leads in ally case, the measurement technique was most robust with leads I, II and V/sub 4/.
高分辨率12导联心电图记录中p波持续时间和位置的可靠检测和测量,通过计算机程序自动进行,该程序包括通过信号平均降噪、25 Hz p波和40 Hz QRS复合的双向滤波,然后,p波开始、p偏移和QRS开始检测算法,允许估计p波持续时间和PQ间隔,该计算机程序在52名志愿者中进行了临床测试。结果表明,虽然在大多数情况下,至少可以在三个引线上进行可靠的纵波检测,但使用引线I, II和V/sub 4/时测量技术最稳健。
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引用次数: 2
Acoustic sensors for respiratory air flow-from basic principles to clinical evaluation and industrialization 呼吸气流声传感器——从基本原理到临床评价和产业化
B. Hok
Acoustic detection principles are potentially useful for monitoring of respiratory air flow. One passive sensor has been evaluated in three clinical investigations. Its sensitivity and specificity to apnea detection was found to be significantly higher than, e.g. pulse oximetry. The sensor is now being industrialized. Furthermore, a new principle for noncontacting detection of respiratory flow is demonstrated.
声学检测原理对监测呼吸气流有潜在的用处。一种无源传感器已经在三个临床研究中进行了评估。其对呼吸暂停检测的敏感性和特异性明显高于脉搏血氧仪等。这种传感器现在正在工业化。提出了一种新的呼吸流量非接触检测原理。
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引用次数: 2
A microfabricated planar magnetic particle separator with optically inspectable flow channel 一种具有光学可检测流道的微加工平面磁粉分离器
Wenjin Zhang, C. Ahn
A new planar magnetic particle separator with opening flow channel which can separate magnetic beads from suspended bio-fluids has been realized on silicon and Pyrex glass wafers, using microfabrication techniques. Electromagnets and fluid channels are separately fabricated, and then two components are bonded together to construct the separator using electrostatic wafer bonding techniques. Separation tests are performed by flowing a suspension containing magnetic beads through the channel. Magnetic beads binding with protein or enzyme which are suspended in bio-liquid solutions have been successfully separated from the suspensions using the realized magnetic separator, while the separation process can be continuously monitored with optical beams. The planar magnetic particle separators microfabricated in this work have a high potential in biomedical or biological applications, especially in separating small amounts of cells, enzyme, protein, or DNA that are bound or marked on magnetic beads.
采用微细加工技术,在硅片和耐热玻璃晶片上实现了一种新型的具有开口流道的平面磁粉分离器,可从悬浮生物流体中分离磁珠。首先分别制作电磁铁和流体通道,然后利用静电晶圆键合技术将两个元件粘接在一起构成分离器。分离试验是通过使含有磁珠的悬浮液流过通道来进行的。利用所实现的磁选器,成功地将悬浮在生物液体溶液中的与蛋白质或酶结合的磁珠从悬浮液中分离出来,并利用光束对分离过程进行连续监测。本研究所制备的平面磁颗粒分离器在生物医学或生物学方面具有很高的应用潜力,特别是在分离结合或标记在磁珠上的少量细胞、酶、蛋白质或DNA方面。
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引用次数: 1
Novel nonisotopic gibberellin DNA probe labeling and fluorescent detection system 新型非同位素赤霉素DNA探针标记及荧光检测系统
Y. Yamada, H. Sugiyama, N. Kagiyama, M. Momiyama, Y. Kondou
A novel highly sensitive nonisotopic DNA labeling with Gibberellin (GA4) and a new fluorescent fast detection system have been developed. The DNA is labeled with GA4 linked to dUTP via an 8 atom linker spacer (GA4-[8]-dUTP) by a random-primed method. Following hybridization of a membrane-bound target DNA with a GA4-labeled probe, the hybrids are detected by the ELISA principle using anti-GA4 Fab fragments conjugated to alkaline phosphatase (ALP). The novel GA4 system allows the detection of 0.1 pg homologous DNA within several hours in dot- and Southern-blots on nylon membranes using a new fluorochrome, 2-hydroxy-3-naphthoic-acid-2'-phenyl-anilide phosphate (HNPP), as the substrate for ALP. The newly developed GA4 system and HNPP fluorescent detection method exhibits not only high sensitivity and low background, but the sensitivity of DNA detection is identical over a wide range of ratio of GA4-dUTP to dUTP in labeling reaction.
利用赤霉素(GA4)建立了一种新的高灵敏度非同位素DNA标记和荧光快速检测系统。DNA被标记为GA4,通过8个原子连接间隔(GA4-[8]-dUTP)通过随机引物的方法连接到dUTP。将膜结合的靶DNA与ga4标记的探针杂交后,利用与碱性磷酸酶(ALP)结合的抗ga4 Fab片段,通过ELISA原理检测杂交产物。新的GA4系统使用一种新的荧光染料,2-羟基-3-萘酸-2'-苯基苯胺磷酸(HNPP)作为ALP的底物,可以在几个小时内在尼龙膜上的点印迹和southern印迹中检测到0.1 pg的同源DNA。新开发的GA4体系和HNPP荧光检测方法不仅具有高灵敏度和低本底的特点,而且在标记反应中GA4-dUTP与dUTP的比例很大的范围内,DNA检测的灵敏度是相同的。
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引用次数: 0
Intraneural microstimulation for enhanced prosthetic control using a peripheral nerve interface 利用外周神经界面增强假肢控制的神经内微刺激
D. Edell, R. Riso, L. Devaney, B. Larsen, M. Koris, D. DeLorenzo
The functionality of prosthetic arms would be enhanced by incorporating cognitive feedback about tactile events, grasp force and hand position. This goal may best be met by interfacing directly with the peripheral nerves in the prosthesis user's residual limb. We are presently exploring a variety of interface designs based on arrays of fine wires. The efficacy of nerve fiber activation and the biocompatibility of the arrays are being studied by chronic implantation into the transected tibial nerve in the rabbit. A behavioral eye blink conditioning paradigm was implemented to assess the implant functionality over time. Our ultimate goal is to achieve stable activation of single tactile afferents. Results show that stable nerve activation with thresholds on the order of 2 nC/stimulus phase (100 /spl mu/s stimulus), can be achieved for a long period (at least 15 months has been verified in one animal that is still being observed). Using the eye blink test paradigm, it is not possible to verify if the behavioral threshold represents single fiber activation. However, in view of the low stimulus threshold, it is likely that only a few fibers are being activated.
通过结合触觉事件、抓握力和手的位置的认知反馈,假肢手臂的功能将得到增强。这一目标最好通过直接与假肢使用者残肢的周围神经连接来实现。我们目前正在探索各种基于细线阵列的接口设计。通过在兔胫骨神经内长期植入,研究了神经纤维的激活效果和生物相容性。采用行为眨眼条件反射范式来评估植入物随时间的功能。我们的最终目标是实现单个触觉传入的稳定激活。结果表明,神经激活阈值在2 nC/刺激期(100 /spl mu/s刺激)左右,可以实现长时间的稳定激活(至少在一只仍在观察的动物中得到了15个月的验证)。使用眨眼测试范例,不可能验证行为阈值是否代表单个纤维激活。然而,鉴于低刺激阈值,很可能只有少数纤维被激活。
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引用次数: 5
Imaging of the head using induced current impedance tomography 使用感应电流阻抗断层成像的头部
C. Towers, I. Freeston, R. C. Tozer
The use of induced current electrical impedance imaging to detect epileptic foci has been simulated. The simulation uses a three dimensional finite element model to determine the sensitivity required of the measuring system in order to detect an epileptic focus represented as a cube of side 10 mm with a 10% change of conductivity. It is shown that the system can image foci near the edge of the region, but is less successful near the centre.
模拟了用感应电流电阻抗成像检测癫痫病灶的方法。模拟使用三维有限元模型来确定测量系统所需的灵敏度,以便检测以边长为10mm的立方体表示的癫痫病灶,其电导率变化为10%。结果表明,该系统可以对区域边缘附近的焦点进行成像,但对中心附近的焦点成像效果较差。
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引用次数: 2
Transmembrane potential distribution in a spherical heart during field stimulation: a 3-D finite element bidomain model 场刺激下球形心脏跨膜电位分布:三维有限元双域模型
Qiuju Huang, F. Claydon
The objective of this study is to utilize a 3-D finite element bidomain model to examine the transmembrane potential of the heart that arises from an external electric field. The myocardium is modeled as a spherical shell with an anisotropy conductivity ratio of 9.2 in the intracellular space and 2.6 in the extracellular space. Fiber rotation from the epicardium to endocardium is also incorporated into the finite element model. The results from an applied uniform electric field external to the heart indicate that the transmembrane potential is sensitive to the orientation of the cardiac fibers to the external field. This result suggests that cardiac fiber orientation is an important feature when using models to simulate the heart's response to external applied fields.
本研究的目的是利用三维有限元双域模型来检查由外电场引起的心脏跨膜电位。心肌模型为球壳,细胞内各向异性电导率为9.2,细胞外各向异性电导率为2.6。从心外膜到心内膜的纤维旋转也被纳入有限元模型。在心脏外施加均匀电场的结果表明,跨膜电位对心脏纤维对外场的取向很敏感。这一结果表明,当使用模型模拟心脏对外部电场的反应时,心脏纤维取向是一个重要的特征。
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引用次数: 0
Continuous localization of ectopic left ventricular activation sites by means of a two-dimensional representation of body surface QRS integral maps 利用体表QRS积分图的二维表示对异位左心室激活部位进行连续定位
M. Potse, A. Linnenbank, A. SippensGroenewegen, C. Grimbergen
By means of Karhunen-Loeve transformation it is possible to represent electrocardiographic body surface QRS integral maps using only two coefficients while retaining more than 80% of information. These coefficients are computed for a number of mean paced QRS integral maps, which were acquired from well-defined ventricular segments and are contained in a database used for localization of the origin of ventricular tachycardia. It is shown that these two coefficients are sufficient to compute the pacing site in a continuous fashion, without reference to a limited number of segments. These coefficients facilitate and improve the ventricular tachycardia localization procedure based on using the database of paced QRS integral maps, by establishing the relation between QRS integral map and corresponding site of origin.
通过Karhunen-Loeve变换,可以仅使用两个系数表示心电图体表QRS积分图,同时保留80%以上的信息。这些系数是为许多平均节奏QRS积分图计算的,这些积分图是从定义明确的心室段获得的,并包含在用于定位室性心动过速起源的数据库中。结果表明,这两个系数足以以连续的方式计算起搏位置,而无需参考有限数量的片段。这些系数通过建立QRS积分图与相应原点之间的关系,促进和改进了基于有节奏QRS积分图数据库的室性心动过速定位过程。
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引用次数: 5
Piezo-dynamometry of foot-to-floor interactions during locomotion 运动过程中脚与地板相互作用的压电动力学
V. Macellari, C. Giacomozzi, E. Scattolini, A. Cappozzo
A compound instrument made of a six component strain gauged force platform and a pressure platform rigidly connected to it was developed aimed at analysing the foot-to-floor interaction during locomotion. Integrating these two instruments allowed the simultaneous estimation of resultant ground reaction components (vertical and shear resultant force components, free moment and location of the centre of pressure), pressure distribution throughout the foot-floor contact area, the trajectory of the centre of pressure superimposed to the contact area (foot print). All of these quantities were made available in each sampled instant of time during the execution of the locomotor act. In addition, the readings provided by the force plate allowed for a time dependent calibration of the pressure sensors. This permitted the authors to overcome the problems associated with the non-linear properties and hysteresis of the relevant sensing material.
研制了一种由六分量应变测力平台和与之刚性连接的压力平台组成的复合仪器,用于分析运动过程中脚与地板的相互作用。将这两种仪器集成在一起,可以同时估计地面合力分量(垂直和剪切合力分量、自由力矩和压力中心的位置)、整个脚-地板接触区域的压力分布、压力中心叠加到接触区域的轨迹(脚印)。所有这些量都是在执行运动动作的每个采样时刻提供的。此外,力板提供的读数允许对压力传感器进行随时间的校准。这使得作者能够克服与相关传感材料的非线性特性和滞后相关的问题。
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引用次数: 2
Dynamic spectral analysis of arterial Doppler blood flow signals using time-frequency representations 动态频谱分析动脉多普勒血流信号使用时频表示
X. Long, J.W. Lee, V. C. Roberts
Doppler signals from a disturbed arterial blood flow are very likely to be nonstationary for they usually have a time-varying frequency structure caused by a complicated flow pattern. Therefore conventional Fourier analysis based methods are unsuitable for this application. This paper introduces the application of joint time-frequency analysis (TFA) to estimate the frequency dynamics of arterial Doppler blood flow signals and to detect the presence of small stenoses of the artery. The short time Fourier transform (STFT), Wigner distribution (WD), and Choi-Williams distribution (CWD) were investigated. The results of synthetic signal analysis show that the STFT, WD and CWD follow the transient changes of frequency well, and the STFT and CWD also behave well for multicomponent signals. Doppler spectra were obtained by means of the FFT, STFT, WD, and CWD for different flow rates and positions along a simulated blood vessel for stenoses ranging from 2% to 25% cross sectional area reduction (CSAR) using a blood analogue. The results indicate that all of these methods are to some extent capable of detecting stenoses as small as 2% CSAR by identifying the existence of negative frequency components in the spectra, and the CWD appears at this stage to be superior to the STFT and WD.
受到干扰的动脉血流的多普勒信号很可能是非平稳的,因为它们通常具有由复杂的血流模式引起的时变频率结构。因此,传统的基于傅里叶分析的方法不适合这种应用。本文介绍了联合时频分析(TFA)在估计动脉多普勒血流信号的频率动态和检测动脉小血管狭窄中的应用。研究了短时傅里叶变换(STFT)、Wigner分布(WD)和Choi-Williams分布(CWD)。综合信号分析结果表明,STFT、WD和CWD对频率的瞬态变化具有良好的跟踪性,对于多分量信号,STFT和CWD也表现良好。通过FFT、STFT、WD和CWD获得不同流速和位置的多普勒频谱,用于使用血液模拟物的狭窄范围为2%至25%的横截面积减少(CSAR)。结果表明,通过识别光谱中负频率成分的存在,这些方法都能在一定程度上检测到最小为2% CSAR的狭窄,并且CWD在这个阶段表现出优于STFT和WD。
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引用次数: 2
期刊
Proceedings of 18th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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