首页 > 最新文献

Ophthalmic Technologies XXIX最新文献

英文 中文
Front Matter: Volume 10858 封面:卷10858
Pub Date : 2019-05-23 DOI: 10.1117/12.2531204
The Pascal Rol Lecture on Ophthalmic Technologies is presented by a leading researcher in ophthalmology with a strong interest and pioneering research contributions to the field of ophthalmic technologies. This invited lecture is intended to trigger further development of ophthalmic technologies by stimulating discussions between basic scientists, engineers, and clinicians. The 2019 lecture was supported by Johnson and Johnson Vision the
帕斯卡·罗尔眼科技术讲座由一位对眼科技术领域有着浓厚兴趣和开创性研究贡献的眼科领先研究人员介绍。本次特邀讲座旨在通过激发基础科学家、工程师和临床医生之间的讨论,推动眼科技术的进一步发展。2019年的讲座得到了强生视觉基金会的支持
{"title":"Front Matter: Volume 10858","authors":"","doi":"10.1117/12.2531204","DOIUrl":"https://doi.org/10.1117/12.2531204","url":null,"abstract":"The Pascal Rol Lecture on Ophthalmic Technologies is presented by a leading researcher in ophthalmology with a strong interest and pioneering research contributions to the field of ophthalmic technologies. This invited lecture is intended to trigger further development of ophthalmic technologies by stimulating discussions between basic scientists, engineers, and clinicians. The 2019 lecture was supported by Johnson and Johnson Vision the","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116121176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First clinical application of low cost portable OCT system (Conference Presentation) 低成本便携式OCT系统的首次临床应用(会议介绍)
Pub Date : 2019-03-13 DOI: 10.1117/12.2507936
Ge Song, Sanghoon Kim, Michael Crose, Brian Cox, Evan T. Jelly, J. N. Ulrich, Adam Wax
Optical coherence tomography (OCT) is currently recognized as the gold standard for identifying retinal structural abnormalities in ophthalmology. However, its availability is often limited to large eye centers and research labs due to its high cost and lack of portability. We present a low-cost, portable spectral-domain OCT system with a total cost of materials under $6,000. Compared to current commercial systems, our design offers 50% size reduction and over 80% cost reduction. Image acquisition interface is incorporated and displayed onto a mounted 7-inch touchscreen. Human retinal imaging is demonstrated, and performance is compared with a commercial OCT system. Based on contrast-to-noise ratio analysis, the low-cost OCT demonstrates comparable imaging capabilities.
光学相干断层扫描(OCT)目前被认为是识别眼科视网膜结构异常的金标准。然而,由于其高成本和缺乏便携性,它的可用性通常仅限于大型眼科中心和研究实验室。我们提出了一种低成本、便携的光谱域OCT系统,总材料成本低于6000美元。与目前的商用系统相比,我们的设计可以缩小50%的尺寸,降低80%以上的成本。图像采集接口集成并显示在安装的7英寸触摸屏上。演示了人类视网膜成像,并与商用OCT系统进行了性能比较。基于噪比分析,低成本OCT具有相当的成像能力。
{"title":"First clinical application of low cost portable OCT system (Conference Presentation)","authors":"Ge Song, Sanghoon Kim, Michael Crose, Brian Cox, Evan T. Jelly, J. N. Ulrich, Adam Wax","doi":"10.1117/12.2507936","DOIUrl":"https://doi.org/10.1117/12.2507936","url":null,"abstract":"Optical coherence tomography (OCT) is currently recognized as the gold standard for identifying retinal structural abnormalities in ophthalmology. However, its availability is often limited to large eye centers and research labs due to its high cost and lack of portability. We present a low-cost, portable spectral-domain OCT system with a total cost of materials under $6,000. Compared to current commercial systems, our design offers 50% size reduction and over 80% cost reduction. Image acquisition interface is incorporated and displayed onto a mounted 7-inch touchscreen. Human retinal imaging is demonstrated, and performance is compared with a commercial OCT system. Based on contrast-to-noise ratio analysis, the low-cost OCT demonstrates comparable imaging capabilities.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127272263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spherical aberration of the crystalline lens measured in-vitro using an LRT-OCT system (Conference Presentation) 用LRT-OCT系统体外测量晶状体的球差(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2508664
Siobhan Williams, M. Ruggeri, Bianca Maceo Heilman, Yu-Cherng Chang, Ashik Mohamed, G. Sravani, C. Rowaan, Alex Gonzalez, A. Ho, J. Parel, F. Manns
Anatomical changes of the growing crystalline lens influence its refractive development, including power and spherical aberration. We have recently developed a new instrument that characterizes both the optical and biometric properties of the lens in-vitro by merging Ray-Tracing Aberrometry (RTA) with three-dimensional OCT imaging. In this abstract, we describe the application of the RTA to the measurement of lens spherical aberration.Experiments were performed on 54 isolated human lenses (age: 0.25 to 56 years). The system was programmed to sequentially deliver the probing beam through the lens using a raster scan pattern of 13 × 13 transversal positions spaced 0.5 mm apart. Exit rays were imaged after exiting the tissue chamber at 9 different axial positions (ΔZ = 0 mm to 8 mm) in 1 mm intervals. A total of 1,521 spot images were acquired per lens. All data was automatically analyzed using custom software we developed in MATLAB. Exit ray slopes over a 6 mm pupil were used to determine Zernike wavefront coefficients up to the sixth order. The 4th order Zernike coefficient Z[4,0] was used to measure primary spherical aberration (SA). The results suggest that spherical aberration of the growing lens becomes more negative before adulthood and less negative after around age 30. The data is consistent with results from in-vivo studies that suggest the lens spherical aberration becomes less negative in older lenses (>30 years).
晶状体生长过程中的解剖变化影响其折射发展,包括功率和球差。我们最近开发了一种新的仪器,通过将射线追踪像差法(RTA)与三维OCT成像相结合,来表征晶状体的光学和生物特征。本文介绍了RTA在透镜球差测量中的应用。实验采用54例离体人体晶状体(年龄:0.25 ~ 56岁)进行。该系统被编程为使用13 × 13个间距为0.5 mm的横向位置的光栅扫描模式,顺序地将探测光束通过透镜。以1mm的间隔,在9个不同的轴向位置(ΔZ = 0 mm ~ 8 mm)离开组织腔后成像。每个镜头共获得1521张点图像。所有数据使用我们在MATLAB中开发的定制软件自动分析。出口射线斜度超过6毫米的瞳孔被用来确定泽尼克波前系数高达六阶。采用四阶泽尼克系数Z[4,0]测量初级球差(SA)。结果表明,生长晶状体的球差在成年前趋于负,而在30岁左右后趋于负。该数据与体内研究的结果一致,该结果表明,年龄较大(>30年)的晶状体球差变得不那么负。
{"title":"Spherical aberration of the crystalline lens measured in-vitro using an LRT-OCT system (Conference Presentation)","authors":"Siobhan Williams, M. Ruggeri, Bianca Maceo Heilman, Yu-Cherng Chang, Ashik Mohamed, G. Sravani, C. Rowaan, Alex Gonzalez, A. Ho, J. Parel, F. Manns","doi":"10.1117/12.2508664","DOIUrl":"https://doi.org/10.1117/12.2508664","url":null,"abstract":"Anatomical changes of the growing crystalline lens influence its refractive development, including power and spherical aberration. We have recently developed a new instrument that characterizes both the optical and biometric properties of the lens in-vitro by merging Ray-Tracing Aberrometry (RTA) with three-dimensional OCT imaging. In this abstract, we describe the application of the RTA to the measurement of lens spherical aberration.\u0000\u0000Experiments were performed on 54 isolated human lenses (age: 0.25 to 56 years). The system was programmed to sequentially deliver the probing beam through the lens using a raster scan pattern of 13 × 13 transversal positions spaced 0.5 mm apart. Exit rays were imaged after exiting the tissue chamber at 9 different axial positions (ΔZ = 0 mm to 8 mm) in 1 mm intervals. A total of 1,521 spot images were acquired per lens. All data was automatically analyzed using custom software we developed in MATLAB. Exit ray slopes over a 6 mm pupil were used to determine Zernike wavefront coefficients up to the sixth order. The 4th order Zernike coefficient Z[4,0] was used to measure primary spherical aberration (SA). The results suggest that spherical aberration of the growing lens becomes more negative before adulthood and less negative after around age 30. The data is consistent with results from in-vivo studies that suggest the lens spherical aberration becomes less negative in older lenses (>30 years).","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115844298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel ergonomic optical coherence tomography probe optimized for supine handheld angiography of young children and infants (Conference Presentation) 一种新型的符合人体工程学的光学相干断层扫描探头,用于婴幼儿仰卧位手持式血管造影(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2508034
C. Viehland, Xi Chen, Du Tran-Viet, G. Waterman, Moseph Jackson-Atogi, C. Toth, J. Izatt
Optical coherence tomography angiography (OCTA) is an extension of OCTA that allows for non-invasive imaging of the retinal microvasculature. OCTA imaging of adult retinal diseases is area of active research in ophthalmology as OCTA can provide insight into the pathogenesis of many retinal diseases. Like these adult diseases, pediatric diseases such as retinopathy of prematurity (ROP) have a primarily vascular pathogenesis. However, table top OCTA systems require compliant, seated subjects and cannot be used on infants and young children. In this manuscript we describe the development of a non-contact handheld OCTA (HH-OCTA) probe for imaging of young children and infants in the operating room. The probe utilizes a novel, diverging light on the scanner optical design that provides improved performance over a traditional OCT scanner design. While most handheld OCT probes are designed to be held by the side of the case or by a handle, our operators tend to prefer to grip probes by the tip of the probe for supine imagine. The ergonomics of the HH-OCTA probe were designed to match this grip. The HH-OCTA probe used a 200 kHz OCT engine, has a motorized stage that provides +10 to -10 D refractive error correction, and weighs 700g. Initial OCTA imaging was performed in 9 children or infants during exam under anesthesia. The HH-OCTA images provide visualization of the retinal microvasculature in both normal and pathological eyes.
光学相干断层扫描血管造影(OCTA)是OCTA的延伸,允许视网膜微血管的非侵入性成像。成人视网膜疾病的OCTA成像是眼科研究的活跃领域,因为OCTA可以深入了解许多视网膜疾病的发病机制。像这些成人疾病一样,儿科疾病如早产儿视网膜病变(ROP)主要是血管发病。然而,桌面OCTA系统需要顺从的、坐着的受试者,不能用于婴幼儿。在这篇手稿中,我们描述了一种非接触式手持式OCTA (HH-OCTA)探头的发展,用于在手术室中对幼儿和婴儿进行成像。该探头在扫描仪光学设计上采用了一种新颖的发散光,比传统的OCT扫描仪设计提供了更好的性能。虽然大多数手持式OCT探头被设计为由外壳侧面或手柄握住,但我们的操作员倾向于通过探头尖端握住探头,以进行仰卧想象。HH-OCTA探针的人体工程学设计与这种抓地力相匹配。HH-OCTA探针使用200 kHz OCT发动机,具有提供+10至-10 D屈光误差校正的机动级,重700克。9例儿童或婴儿在麻醉下检查时进行了首次OCTA成像。HH-OCTA图像提供了正常和病理眼睛视网膜微血管的可视化。
{"title":"A novel ergonomic optical coherence tomography probe optimized for supine handheld angiography of young children and infants (Conference Presentation)","authors":"C. Viehland, Xi Chen, Du Tran-Viet, G. Waterman, Moseph Jackson-Atogi, C. Toth, J. Izatt","doi":"10.1117/12.2508034","DOIUrl":"https://doi.org/10.1117/12.2508034","url":null,"abstract":"Optical coherence tomography angiography (OCTA) is an extension of OCTA that allows for non-invasive imaging of the retinal microvasculature. OCTA imaging of adult retinal diseases is area of active research in ophthalmology as OCTA can provide insight into the pathogenesis of many retinal diseases. Like these adult diseases, pediatric diseases such as retinopathy of prematurity (ROP) have a primarily vascular pathogenesis. However, table top OCTA systems require compliant, seated subjects and cannot be used on infants and young children. In this manuscript we describe the development of a non-contact handheld OCTA (HH-OCTA) probe for imaging of young children and infants in the operating room. The probe utilizes a novel, diverging light on the scanner optical design that provides improved performance over a traditional OCT scanner design. While most handheld OCT probes are designed to be held by the side of the case or by a handle, our operators tend to prefer to grip probes by the tip of the probe for supine imagine. The ergonomics of the HH-OCTA probe were designed to match this grip. The HH-OCTA probe used a 200 kHz OCT engine, has a motorized stage that provides +10 to -10 D refractive error correction, and weighs 700g. Initial OCTA imaging was performed in 9 children or infants during exam under anesthesia. The HH-OCTA images provide visualization of the retinal microvasculature in both normal and pathological eyes.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133474574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ocular fundus imaging with transmitted light (Conference Presentation) 眼底透射光成像(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2507989
Timothy D. Weber, J. Mertz
Widefield ocular fundus imaging is conventionally performed in a reflection geometry. In this configuration, back-reflections from inner retinal layers, such as the nerve fiber layer, the inner limiting membrane, or even the anterior walls of large blood vessels, are often encountered, and may obscure the visibility of deeper features. Moreover, spectroscopic quantification of endogenous chromophores is complicated since the final image is a summation of reflections from several fundus layers (i.e. no single absorption pathlength can safely be assumed). Researchers have sought to model the fundus reflections, however the models are sensitive to the populations used and particular imaging platform. In theory, unwanted superficial reflections could be avoided and light path modeling could be simplified by adopting a transmission imaging geometry. We present an alternative transillumination fundus imaging strategy based on deeply penetrating near-infrared (NIR) light delivered transcranial near the subject’s temple. A portion of this light diffuses through bone and illuminates the posterior eye not from the front, as with conventional methods, but rather mostly from behind. As such, we image light transmitted through the fundus rather than back-reflected off multiple fundus layers. This single-pass measurement geometry simplifies absorption pathlength considerations and provides complementary information to fundus reflectometry. The use of NIR light enables imaging as deep as the choroid. Importantly, the technique is compatible with reflection-based techniques and we have shown that it works well with a commercial non-mydriatic fundus camera. Combining information from these two illumination approaches should improve spectroscopic analysis of the fundus.
广角眼底成像通常以反射几何形状进行。在这种情况下,经常会遇到视网膜内层(如神经纤维层、内层限制膜,甚至大血管前壁)的反向反射,这可能会模糊更深层特征的可见性。此外,内源性发色团的光谱定量是复杂的,因为最终的图像是来自几个眼底层的反射的总和(即不能安全地假设单一的吸收路径长度)。研究人员试图建立眼底反射的模型,然而模型对使用的人群和特定的成像平台很敏感。理论上,可以避免不必要的表面反射,并且通过采用透射成像几何结构可以简化光路建模。我们提出了一种替代的眼底透照成像策略,该策略基于深穿透近红外(NIR)光在受试者太阳穴附近经颅传输。一部分光线通过骨头扩散,并不是像传统方法那样从前面照射后眼,而是大部分从后面照射。因此,我们成像通过眼底传输的光,而不是多个眼底层的反向反射。这种单通道测量几何简化了吸收路径长度的考虑,并为眼底反射计提供了补充信息。使用近红外光可以成像到脉络膜深处。重要的是,该技术与基于反射的技术兼容,我们已经证明它与商用无散瞳眼底相机一起工作得很好。结合这两种照明方法的信息可以改善眼底的光谱分析。
{"title":"Ocular fundus imaging with transmitted light (Conference Presentation)","authors":"Timothy D. Weber, J. Mertz","doi":"10.1117/12.2507989","DOIUrl":"https://doi.org/10.1117/12.2507989","url":null,"abstract":"Widefield ocular fundus imaging is conventionally performed in a reflection geometry. In this configuration, back-reflections from inner retinal layers, such as the nerve fiber layer, the inner limiting membrane, or even the anterior walls of large blood vessels, are often encountered, and may obscure the visibility of deeper features. Moreover, spectroscopic quantification of endogenous chromophores is complicated since the final image is a summation of reflections from several fundus layers (i.e. no single absorption pathlength can safely be assumed). Researchers have sought to model the fundus reflections, however the models are sensitive to the populations used and particular imaging platform. In theory, unwanted superficial reflections could be avoided and light path modeling could be simplified by adopting a transmission imaging geometry. We present an alternative transillumination fundus imaging strategy based on deeply penetrating near-infrared (NIR) light delivered transcranial near the subject’s temple. A portion of this light diffuses through bone and illuminates the posterior eye not from the front, as with conventional methods, but rather mostly from behind. As such, we image light transmitted through the fundus rather than back-reflected off multiple fundus layers. This single-pass measurement geometry simplifies absorption pathlength considerations and provides complementary information to fundus reflectometry. The use of NIR light enables imaging as deep as the choroid. Importantly, the technique is compatible with reflection-based techniques and we have shown that it works well with a commercial non-mydriatic fundus camera. Combining information from these two illumination approaches should improve spectroscopic analysis of the fundus.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115366052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Axial motion corrected constant linear velocity spiral scan OCT with dynamic focusing for high resolution wide field corneal and anterior chamber imaging (Conference Presentation) 轴向运动校正恒线速度螺旋扫描OCT动态聚焦用于高分辨率宽视场角膜和前房成像(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2510164
R. McNabb, Yuxiao Wei, Moseph Jackson-Atogi, Alice S Liu, O. Carrasco-Zevallos, J. Izatt, A. Kuo
Imaging the entire human cornea with a conventional OCT system configuration requires trade-offs between resolution and depth-of-focus because the cornea is curved over a depth of approximately 4 mm. These system trade-offs result in image quality variations in the corneal image such as a bright apex surrounded by decreasing intensity as the cornea curves away from the apex. These intensity changes cause non-biological ambiguities in interpreting the image, make it difficult to see anatomy in the dim areas, and make automated surface detection difficult in the periphery. To address this problem, we developed a continuously focusing corneal OCT system coupled with a constant linear velocity (CLV) spiral scan pattern that is able to better maintain focus from the apex to the deeper cornea during a scan. The continuous focusing was implemented by introducing a focusing telescope on a motorized stage into the sample arm and matching the translation of the telescope with the CLV scan as it spiraled from the corneal apex outwards. Orthogonal B-scans prior to volume acquisition were used as a reference to estimate and correct motion that occurred during the subsequent CLV scan. A consented subject was imaged, and the resultant image showed increased intensity in the peripheral and deeper cornea and anterior chamber. Continuous focusing with CLV spiral scanning is a promising design change to OCT systems allowing adequate focus over relatively large depths such as for scanning the human cornea.
使用传统的OCT系统配置成像整个人类角膜需要在分辨率和聚焦深度之间进行权衡,因为角膜在大约4毫米的深度上弯曲。这些系统的权衡导致图像质量的变化,在角膜图像,如明亮的顶点周围的降低强度,因为角膜曲线远离顶点。这些强度变化在解释图像时导致非生物模糊性,使得难以在模糊区域看到解剖结构,并且难以在外围进行自动表面检测。为了解决这个问题,我们开发了一种连续聚焦的角膜OCT系统,加上恒定线速度(CLV)螺旋扫描模式,能够在扫描期间更好地保持从顶点到深层角膜的焦点。连续聚焦是通过在样品臂上的电动平台上引入聚焦望远镜来实现的,当望远镜从角膜尖端向外旋转时,将望远镜的平移与CLV扫描相匹配。体积采集前的正交b扫描被用作估计和纠正随后CLV扫描期间发生的运动的参考。对同意的受试者进行成像,所得图像显示外周、深层角膜和前房的强度增加。连续聚焦与CLV螺旋扫描是一个有前途的OCT系统的设计变化,允许足够的聚焦在相对较大的深度,如扫描人类角膜。
{"title":"Axial motion corrected constant linear velocity spiral scan OCT with dynamic focusing for high resolution wide field corneal and anterior chamber imaging (Conference Presentation)","authors":"R. McNabb, Yuxiao Wei, Moseph Jackson-Atogi, Alice S Liu, O. Carrasco-Zevallos, J. Izatt, A. Kuo","doi":"10.1117/12.2510164","DOIUrl":"https://doi.org/10.1117/12.2510164","url":null,"abstract":"Imaging the entire human cornea with a conventional OCT system configuration requires trade-offs between resolution and depth-of-focus because the cornea is curved over a depth of approximately 4 mm. These system trade-offs result in image quality variations in the corneal image such as a bright apex surrounded by decreasing intensity as the cornea curves away from the apex. These intensity changes cause non-biological ambiguities in interpreting the image, make it difficult to see anatomy in the dim areas, and make automated surface detection difficult in the periphery. To address this problem, we developed a continuously focusing corneal OCT system coupled with a constant linear velocity (CLV) spiral scan pattern that is able to better maintain focus from the apex to the deeper cornea during a scan. The continuous focusing was implemented by introducing a focusing telescope on a motorized stage into the sample arm and matching the translation of the telescope with the CLV scan as it spiraled from the corneal apex outwards. Orthogonal B-scans prior to volume acquisition were used as a reference to estimate and correct motion that occurred during the subsequent CLV scan. A consented subject was imaged, and the resultant image showed increased intensity in the peripheral and deeper cornea and anterior chamber. Continuous focusing with CLV spiral scanning is a promising design change to OCT systems allowing adequate focus over relatively large depths such as for scanning the human cornea.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116244883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Towards in vivo imaging of the mouse cone photoreceptors (Conference Presentation) 小鼠视锥细胞光感受器的体内成像研究(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2508864
Pengfei Zhang, Eric B. Miller, Suman K. Manna, R. Meleppat, E. Pugh, R. Zawadzki
Vision is the most important sense organ of human, more than 80% of the information from outside world is acquired by vision. Vision starts at the photoreceptors in the retina capturing the visible light photons. There are two general types of photoreceptors, called rods and cones. Rods allow us to see in dim and dark light, cones allow us to perceive fine visual detail and color. To understand physiology of cones, researchers developed many model organisms that allow them to study in details different aspects of photoreceptors function. Specifically, mice play a central role in basic vision science research. However, one should keep in mind that mice have rod dominant retinas which is different from human cone dominant retinas near fovea. As one of the consequence in vivo imaging of cones in humans is relatively easy in periphery, and cone mosaic was the first cellular structure that was reported to be seen by optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO), especially with implementation of adaptive optics (AO)[1]. However, just recently researchers started to visualize human rods which are smaller than cones [2, 3]. In case of mouse retinal imaging, it is quite the opposite situation. There have been recent reports of imaging rods mosaic [4-6], but up to date no reports on identifying cones in the images. Given that the cones are twice as big as rods in mice, it is very interesting why one can visualize rods but cannot visualize cones.
视觉是人类最重要的感觉器官,80%以上的外界信息是通过视觉获得的。视觉开始于视网膜中的感光器捕捉可见光光子。一般有两种类型的光感受器,称为视杆细胞和视锥细胞。视杆细胞让我们在昏暗和黑暗的光线下看到东西,视锥细胞让我们感知精细的视觉细节和颜色。为了了解视锥细胞的生理,研究人员开发了许多模式生物,使他们能够详细研究光感受器功能的不同方面。具体来说,小鼠在基础视觉科学研究中发挥着核心作用。然而,我们应该记住,老鼠的视网膜是杆状显性的,这与人类在中央凹附近的视网膜是不同的。因此,人体内视锥细胞的外围成像相对容易,而视锥镶嵌是第一个被光学相干断层扫描(OCT)和扫描激光检眼镜(SLO)观察到的细胞结构,特别是自适应光学(AO)的应用[1]。然而,就在最近,研究人员开始设想人类的杆状体比锥体小[2,3]。在小鼠视网膜成像中,情况则完全相反。最近有关于成像杆镶嵌的报道[4-6],但迄今为止还没有关于在图像中识别锥体的报道。考虑到老鼠的视锥细胞是视杆细胞的两倍大,为什么人们可以看到视杆细胞而不能看到视锥细胞,这是非常有趣的。
{"title":"Towards in vivo imaging of the mouse cone photoreceptors (Conference Presentation)","authors":"Pengfei Zhang, Eric B. Miller, Suman K. Manna, R. Meleppat, E. Pugh, R. Zawadzki","doi":"10.1117/12.2508864","DOIUrl":"https://doi.org/10.1117/12.2508864","url":null,"abstract":"Vision is the most important sense organ of human, more than 80% of the information from outside world is acquired by vision. Vision starts at the photoreceptors in the retina capturing the visible light photons. There are two general types of photoreceptors, called rods and cones. Rods allow us to see in dim and dark light, cones allow us to perceive fine visual detail and color. To understand physiology of cones, researchers developed many model organisms that allow them to study in details different aspects of photoreceptors function. Specifically, mice play a central role in basic vision science research. However, one should keep in mind that mice have rod dominant retinas which is different from human cone dominant retinas near fovea. \u0000As one of the consequence in vivo imaging of cones in humans is relatively easy in periphery, and cone mosaic was the first cellular structure that was reported to be seen by optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO), especially with implementation of adaptive optics (AO)[1]. However, just recently researchers started to visualize human rods which are smaller than cones [2, 3]. In case of mouse retinal imaging, it is quite the opposite situation. There have been recent reports of imaging rods mosaic [4-6], but up to date no reports on identifying cones in the images. Given that the cones are twice as big as rods in mice, it is very interesting why one can visualize rods but cannot visualize cones.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117318045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
In vivo imaging of vitreous opacities with full-eye-length SS-OCT (Conference Presentation) 全眼SS-OCT在体内成像玻璃体混浊(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2509474
I. Grulkowski, Ana Rodríguez-Aramendía, S. Manzanera, Yiwei Chen, Juan Mompeán, F. Díaz-Doutón, J. Pujol, J. Sebag, P. Artal
Transparency of ocular structures is an important factor determining contrast in the retinal image. Although opacities are most commonly formed in the crystalline lens of aging eye (cataract formation), visual function can be also altered by the opacities in the vitreous body. Therefore, macro- and micro-scale visualization of vitreous is clinically relevant since alterations of vitreous organization impact retinal diseases and affect vision. However, optical imaging of the vitreous body is challenging due to its transparency. We demonstrate visualization of vitreous and its opacities in vivo using optical coherence tomography (OCT). We developed a prototype long-depth-range Swept-Source OCT instrument operating at the speed of 30 kA-scans/second and at the central wavelength of 1 μm to perform high-resolution imaging through the entire vitreous depth. The interface with focus-tunable optics has been used to optimize the field of view. 2-D and 3-D OCT data sets of eyes with vitreous opacities were acquired and processed to obtain contrast-enhanced high-resolution images of vitreous. The results demonstrate the ability of the OCT imaging to characterize the opacities that cause floaters. In conclusion, long-depth-range SS-OCT enables volumetric visualization of in vivo microstructural changes in the vitreous body. This instrument might be a useful tool in high-resolution evaluation and surgical management of vitreous opacities.
眼部结构的透明度是决定视网膜图像对比度的重要因素。虽然混浊最常见于老化眼睛的晶状体(白内障形成),但玻璃体中的混浊也会改变视觉功能。因此,玻璃体的宏观和微观可视化具有临床意义,因为玻璃体组织的改变会影响视网膜疾病并影响视力。然而,由于玻璃体的透明度,光学成像具有挑战性。我们展示了使用光学相干断层扫描(OCT)可视化玻璃体及其混浊物。我们开发了一种原型长深度扫描源OCT仪器,工作速度为30 ka扫描/秒,中心波长为1 μm,可在整个玻璃体深度进行高分辨率成像。可调焦光学接口被用来优化视场。获取玻璃体混浊眼的二维和三维OCT数据集,并对其进行处理,获得玻璃体对比度增强的高分辨率图像。结果证明了OCT成像表征引起飞蚊的混浊的能力。总之,长深度范围SS-OCT能够实现玻璃体体内显微结构变化的体积可视化。该仪器可能是高分辨率评估和手术治疗玻璃体混浊的有用工具。
{"title":"In vivo imaging of vitreous opacities with full-eye-length SS-OCT\u0000 (Conference Presentation)","authors":"I. Grulkowski, Ana Rodríguez-Aramendía, S. Manzanera, Yiwei Chen, Juan Mompeán, F. Díaz-Doutón, J. Pujol, J. Sebag, P. Artal","doi":"10.1117/12.2509474","DOIUrl":"https://doi.org/10.1117/12.2509474","url":null,"abstract":"Transparency of ocular structures is an important factor determining contrast in the retinal image. Although opacities are most commonly formed in the crystalline lens of aging eye (cataract formation), visual function can be also altered by the opacities in the vitreous body. Therefore, macro- and micro-scale visualization of vitreous is clinically relevant since alterations of vitreous organization impact retinal diseases and affect vision. However, optical imaging of the vitreous body is challenging due to its transparency. We demonstrate visualization of vitreous and its opacities in vivo using optical coherence tomography (OCT). We developed a prototype long-depth-range Swept-Source OCT instrument operating at the speed of 30 kA-scans/second and at the central wavelength of 1 μm to perform high-resolution imaging through the entire vitreous depth. The interface with focus-tunable optics has been used to optimize the field of view. 2-D and 3-D OCT data sets of eyes with vitreous opacities were acquired and processed to obtain contrast-enhanced high-resolution images of vitreous. The results demonstrate the ability of the OCT imaging to characterize the opacities that cause floaters. In conclusion, long-depth-range SS-OCT enables volumetric visualization of in vivo microstructural changes in the vitreous body. This instrument might be a useful tool in high-resolution evaluation and surgical management of vitreous opacities.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122386963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-invasive and high temporal resolution choroidal and retinal blood flow imaging using laser Doppler holography (Conference Presentation) 激光多普勒全息成像无创、高时间分辨率脉络膜和视网膜血流成像(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2506157
L. Puyo, M. Pâques, M. Fink, J. Sahel, M. Atlan
Monitoring retinal vascularization is crucial to understand the pathophysiology of major diseases affecting the retina. Laser Doppler holography addresses the problem of temporal resolution in blood flow imaging. The method is conceptually close to laser Doppler flowmetry except it uses digital holography which allows for full-field imaging with a simple Mach–Zehnder interferometer. The light backscattered by the retina is combined with a reference field in order to measure the beat frequency spectrum with a very high acquisition frame rate. Wideband measurements of the optical Doppler broadening were performed with a 75 kHz crash test camera. The power spectrum density of the recorded holograms was analyzed with a short-time Fourier transform analysis to reveal local pulsatile flow. By using laser Doppler holography, we were able to image blood flow in vivo and qualitatively in retinal vessels with a temporal resolution down to a few milliseconds which enabled to investigate blood flow profiles in arteries and veins. Additionally the angiographic contrast in power Doppler images has proved sensitive to lateral flow which made possible to image vessels in en-face planes. Finally we showed that laser Doppler holography allows to reveal non-invasively in young and healthy subjects the large vessels of the choroid. To this end we stitched together multiple power Doppler images to form a wide-field laser Doppler holographic panorama.
监测视网膜血管形成对于了解影响视网膜的主要疾病的病理生理至关重要。激光多普勒全息技术解决了血流成像的时间分辨率问题。该方法在概念上接近激光多普勒流量测量,但它使用数字全息,允许用简单的马赫-曾德干涉仪进行全场成像。将视网膜后向散射的光与参考场相结合,以非常高的采集帧率测量拍频。用75khz碰撞测试相机进行了光学多普勒加宽的宽带测量。利用短时傅立叶变换分析记录全息图的功率谱密度,揭示局部脉动流。通过使用激光多普勒全息成像,我们能够在体内和定性地成像视网膜血管中的血流,时间分辨率低至几毫秒,这使得研究动脉和静脉的血流概况成为可能。此外,功率多普勒造影对侧流很敏感,这使得在正面平面上成像血管成为可能。最后,我们发现激光多普勒全息术可以在年轻和健康的受试者中无创地显示脉络膜的大血管。为此,我们将多个功率多普勒图像拼接在一起,形成宽视场激光多普勒全息全景图。
{"title":"Non-invasive and high temporal resolution choroidal and retinal blood flow imaging using laser Doppler holography (Conference Presentation)","authors":"L. Puyo, M. Pâques, M. Fink, J. Sahel, M. Atlan","doi":"10.1117/12.2506157","DOIUrl":"https://doi.org/10.1117/12.2506157","url":null,"abstract":"Monitoring retinal vascularization is crucial to understand the pathophysiology of major diseases affecting the retina. Laser Doppler holography addresses the problem of temporal resolution in blood flow imaging. The method is conceptually close to laser Doppler flowmetry except it uses digital holography which allows for full-field imaging with a simple Mach–Zehnder interferometer. The light backscattered by the retina is combined with a reference field in order to measure the beat frequency spectrum with a very high acquisition frame rate. Wideband measurements of the optical Doppler broadening were performed with a 75 kHz crash test camera. The power spectrum density of the recorded holograms was analyzed with a short-time Fourier transform analysis to reveal local pulsatile flow. By using laser Doppler holography, we were able to image blood flow in vivo and qualitatively in retinal vessels with a temporal resolution down to a few milliseconds which enabled to investigate blood flow profiles in arteries and veins. Additionally the angiographic contrast in power Doppler images has proved sensitive to lateral flow which made possible to image vessels in en-face planes. Finally we showed that laser Doppler holography allows to reveal non-invasively in young and healthy subjects the large vessels of the choroid. To this end we stitched together multiple power Doppler images to form a wide-field laser Doppler holographic panorama.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132572098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Photovoltaic restoration of sight in age-related macular degeneration (Conference Presentation) 光电恢复老年性黄斑变性的视力(会议报告)
Pub Date : 2019-03-13 DOI: 10.1117/12.2508967
D. Palanker, T. Flores, Elton Ho, H. Lorach, Mohajeet B. Bhuckory, T. Kamins, Tiffany W Huang, K. Mathieson
To restore vision in patients who lost photoreceptors due to retinal degeneration, we developed a photovoltaic subretinal prosthesis which converts light into pulsed electric current, stimulating the inner retinal neurons. Visual information is projected onto the retina by video goggles using pulsed near-infrared (880nm) light. This design avoids the use of bulky electronics and wiring, thereby greatly reducing the surgical complexity and allows scaling the implants to thousands of electrodes.We found that similarly to normal vision, retinal response to prosthetic stimulation exhibits flicker fusion at high frequencies (>20Hz), adaptation to static images, antagonistic center-surround receptive fields with non-linear summation of its subunits. In rats, photovoltaic arrays with 55um pixels provided grating visual acuity up to a pixel pitch, which corresponds to about 20/200 acuity in a human eye. In patients with geographic atrophy, implants with 100um pixels provided retinotopically correct pattern percepts with resolution matching the pixel size. With flat pixels of 40um and smaller, stimulation thresholds are becoming prohibitively high. To reduce the pixel size further, we developed a novel honeycomb configuration of the stimulating electrode array with vertical walls separating the active and return electrodes, designed to leverage retinal migration for reducing the subretinal stimulation threshold and electrical cross-talk between neighboring pixels. Scalability, ease of implantation, and high resolution of these arrays open the door to highly functional restoration of sight in retinal degeneration.
为了恢复因视网膜变性而失去光感受器的患者的视力,我们开发了一种光伏视网膜下假体,它将光转化为脉冲电流,刺激视网膜内神经元。视觉信息通过视频护目镜使用脉冲近红外(880nm)光投射到视网膜上。这种设计避免了使用庞大的电子设备和布线,从而大大降低了手术的复杂性,并允许将植入物扩展到数千个电极。我们发现,与正常视觉相似,视网膜对假肢刺激的反应表现为高频闪烁融合(>20Hz),对静态图像的适应,对抗性中心-环绕接受野及其亚基的非线性求和。在大鼠中,55um像素的光伏阵列提供了高达一个像素间距的光栅视敏度,相当于人眼的20/200左右的视敏度。在地理萎缩患者中,100um像素的植入物提供了分辨率与像素大小匹配的视网膜定位正确的模式感知。对于40um及更小的平面像素,刺激阈值变得过高。为了进一步减小像素大小,我们开发了一种新的蜂窝结构的刺激电极阵列,其垂直壁将有源电极和返回电极分开,旨在利用视网膜迁移来降低视网膜下刺激阈值和相邻像素之间的电串扰。这些阵列的可扩展性、易植入性和高分辨率为视网膜变性患者的高功能视力恢复打开了大门。
{"title":"Photovoltaic restoration of sight in age-related macular degeneration (Conference Presentation)","authors":"D. Palanker, T. Flores, Elton Ho, H. Lorach, Mohajeet B. Bhuckory, T. Kamins, Tiffany W Huang, K. Mathieson","doi":"10.1117/12.2508967","DOIUrl":"https://doi.org/10.1117/12.2508967","url":null,"abstract":"To restore vision in patients who lost photoreceptors due to retinal degeneration, we developed a photovoltaic subretinal prosthesis which converts light into pulsed electric current, stimulating the inner retinal neurons. Visual information is projected onto the retina by video goggles using pulsed near-infrared (880nm) light. This design avoids the use of bulky electronics and wiring, thereby greatly reducing the surgical complexity and allows scaling the implants to thousands of electrodes.\u0000We found that similarly to normal vision, retinal response to prosthetic stimulation exhibits flicker fusion at high frequencies (>20Hz), adaptation to static images, antagonistic center-surround receptive fields with non-linear summation of its subunits. In rats, photovoltaic arrays with 55um pixels provided grating visual acuity up to a pixel pitch, which corresponds to about 20/200 acuity in a human eye. In patients with geographic atrophy, implants with 100um pixels provided retinotopically correct pattern percepts with resolution matching the pixel size. \u0000With flat pixels of 40um and smaller, stimulation thresholds are becoming prohibitively high. To reduce the pixel size further, we developed a novel honeycomb configuration of the stimulating electrode array with vertical walls separating the active and return electrodes, designed to leverage retinal migration for reducing the subretinal stimulation threshold and electrical cross-talk between neighboring pixels. Scalability, ease of implantation, and high resolution of these arrays open the door to highly functional restoration of sight in retinal degeneration.","PeriodicalId":204875,"journal":{"name":"Ophthalmic Technologies XXIX","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132578281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
期刊
Ophthalmic Technologies XXIX
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1