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Investigating the Supplementation of Lactococcus Lactis MA5 as an Autochthonous Probiotic in Diets for Hybrid Catfish Ictalurus Punctatus × I. Furcatus: Effects on Growth Performance, Immune Modulation, and Disease Resistance. 杂交斑点鲶鱼饲料中添加乳酸乳球菌MA5作为原生益生菌的研究:对其生长性能、免疫调节和抗病性的影响
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-07 DOI: 10.1007/s12602-026-10930-1
Jing Huang, Caitlin E Older, Taylor I Heckman, Vitor F Silva, Heather Jordan, Matt J Griffin, Peter J Allen, Fernando Y Yamamoto
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引用次数: 0
Probiotic Bacillus Clausii Protects against Amoxicillin-evoked Reproductive Dysfunction through Oxidative Stress Modulation. 克劳氏益生菌通过氧化应激调节防止阿莫西林引起的生殖功能障碍。
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-05 DOI: 10.1007/s12602-025-10891-x
Nadia A El-Fahla, Nahla S El-Shenawy, Noran M Tawfik

Antibiotics are frequently used in livestock, aquaculture, and poultry, resulting in their accumulation in these animals and the surrounding environment. This contamination can present health risks to humans. Research on the harmful effects of antibiotics, especially their potential to disrupt hormones in the male reproductive system, is still in its early phases. This study investigated the impact of exposure to the antibiotic amoxicillin (AMO) on male reproductive health. The aim is to shed light on the potential dangers antibiotics pose to male reproductive health and to emphasize how Bacillus clausii may help protect against the toxic effects of AMO. The study included four groups treated via gavage: control animals, a supplemented group receiving B. clausii (1.25 ml, 1 × 10^9 CFU per mouse per day), a group treated with AMO at 30 mg/kg daily, and a fourth group that received AMO and B. clausii. After 45 days of treatment, blood samples were collected to assess serum activities of superoxide dismutase and catalase and levels of reduced glutathione, malondialdehyde, and testosterone. Testicular tissue samples were analyzed for mechanistic target of rapamycin (mTOR) gene expression and histological changes. This study demonstrates that exposure to AMO negatively impacts male reproductive health by increasing oxidative stress, reducing testosterone levels, causing testicular damage, and downregulating the mTOR expression level. However, supplementation with B. clausii mitigates these harmful effects, likely due to its antioxidant properties. The probiotic reduced oxidative stress markers, improved testosterone levels, and alleviated testicular lesions, thereby upregulating the mTOR expression level, which suggests its potential protective role against antibiotic-induced toxicity.

抗生素经常被用于家畜、水产养殖和家禽,导致它们在这些动物和周围环境中积累。这种污染会给人类带来健康风险。关于抗生素有害影响的研究,特别是它们可能破坏男性生殖系统中的激素的研究,仍处于早期阶段。本研究调查了接触抗生素阿莫西林(AMO)对男性生殖健康的影响。其目的是阐明抗生素对男性生殖健康构成的潜在危险,并强调克劳梭杆菌如何有助于防止AMO的毒性作用。研究分为四组灌胃:对照动物,补充克劳梭菌组(1.25 ml, 1 × 10^9 CFU /小鼠/ d), AMO组(30 mg/kg / d), AMO和克劳梭菌组(4)。治疗45天后,采集血样评估血清超氧化物歧化酶和过氧化氢酶的活性以及还原性谷胱甘肽、丙二醛和睾酮的水平。分析睾丸组织样品雷帕霉素(mTOR)基因表达和组织学变化的机制靶点。本研究表明,暴露于AMO会增加氧化应激,降低睾丸激素水平,导致睾丸损伤,下调mTOR表达水平,从而对男性生殖健康产生负面影响。然而,补充克劳梭菌可以减轻这些有害影响,可能是由于其抗氧化特性。益生菌降低氧化应激标志物,提高睾酮水平,减轻睾丸病变,从而上调mTOR表达水平,提示其对抗生素诱导的毒性具有潜在的保护作用。
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引用次数: 0
Optimization of Fermentation Condition to Enhance Postbiotic Yield and Bio-Functional Efficacy from Lactobacillus-Bifidobacterium Strains Co-Fermentation. 优化发酵条件提高乳酸菌-双歧杆菌共发酵生后产量和生物功能功效
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-05 DOI: 10.1007/s12602-025-10899-3
Zerihun Asefa, Abera Belay, Teferi Damto, Admasu Addi, Mesfin Getachew Tadesse, Endale Amare, Feriehiwote Weldeyohanis Gebremariam, Ibrahim Ahmed Sherif, Henock Woldemichael Woldemariam
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引用次数: 0
Understanding The Role of Lacticaseibacillus rhamnosus in Vaginal Dysbiosis: in Vitro Studies and Clinical Evidence. 了解鼠李糖乳杆菌在阴道生态失调中的作用:体外研究和临床证据。
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1007/s12602-026-10922-1
Benedetta Canala, Luciana Rossi
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引用次数: 0
Postbiotic-Mediated Green Synthesis of Silver Nanoparticles: Revealing Potent Antimicrobial, Anti-Biofilm, Antioxidant, and Anticancer Properties using Paenibacillus xylanexedens. 生物后介导的纳米银绿色合成:揭示木聚糖双胞杆菌有效的抗菌、抗生物膜、抗氧化和抗癌特性。
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1007/s12602-025-10905-8
Sema Yiyit Doğan, Seçil Kaya, Ebru Kondolot Solak
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引用次数: 0
Evaluation of antioxidant activities of bioactive peptides extracted from Curcuma longa and Curcuma caesia from South-eastern and North-Eastern India. 印度东南部和东北部姜黄和黄姜黄生物活性肽的抗氧化活性评价。
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1007/s12602-025-10895-7
Anshula Narayanasamy, Abinaya Kanagaraja, Munusamy Thirumavalavan, Meenakumari Sakthivelu, Raman Pachaiappan

Turmeric, referred to as "Golden Spice of India," belongs to Zingiberaceae family is cultivated in South-eastern and North-eastern regions of India, where it displays the regional diversity. In this study, turmeric rhizomes of Curcuma longa (yellow turmeric) from the South-eastern India and Curcuma caesia (black turmeric) from North-eastern India were collected. Proteins from these rhizome samples were extracted by using four different pH based buffer systems. The sequentially purified peptide filtrates obtained through ultrafiltration with 10 kDa and 3 kDa cut off membranes were quantified and assessed for their antioxidant potential by FRAP, NOS, DPPH and ABTS assays. The results showed that the highest antioxidant activities were observed in the Phosphate (pH 7.0) and Tris-HCl (pH 8.0) buffers. Further analysis of the 3 kDa filtrates from both turmeric samples by using HPLC confirmed the presence of peptides, which were subsequently identified by MALDI-TOF MS. The peptides identified in this study exhibited the promising bioactive potential based on their sequences and showed the less cleaving potential evaluated by insilico peptideCutter analysis. Future research will focus on validating these activities through comprehensive experimental approaches, including cell-based antioxidant, mechanistic studies, and antimicrobial evaluations. Such investigations will be essential to confirm their biological relevance and to determine its suitability for nutraceutical applications.

姜黄,被称为“印度的黄金香料”,属于姜科,种植在印度东南部和东北部地区,在那里它显示出区域多样性。本研究采集了产自印度东南部的黄姜黄和产自印度东北部的黑姜黄的姜黄根茎。从这些根茎样品中提取蛋白质,使用四种不同的pH为基础的缓冲系统。分别用10 kDa和3 kDa切膜进行超滤纯化后的肽滤液,通过FRAP、NOS、DPPH和ABTS测定其抗氧化能力。结果表明,磷酸盐(pH 7.0)和Tris-HCl (pH 8.0)缓冲液的抗氧化活性最高。通过HPLC对两种姜黄样品的3 kDa滤液进行进一步分析,证实了多肽的存在,随后通过MALDI-TOF ms对其进行了鉴定。本研究中鉴定的多肽根据其序列显示出良好的生物活性潜力,并且通过insilico peptideCutter分析显示出较少的切割潜力。未来的研究将集中于通过全面的实验方法来验证这些活性,包括基于细胞的抗氧化、机制研究和抗菌评估。这样的调查对于确认它们的生物学相关性和确定其营养应用的适用性至关重要。
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引用次数: 0
Probiotic Lactobacillus johnsonii RS-7 Alleviates Intestinal Inflammation Via the TLR4/MyD88/NF-κB Signaling Pathway. 益生菌约氏乳杆菌RS-7通过TLR4/MyD88/NF-κB信号通路缓解肠道炎症
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-03 DOI: 10.1007/s12602-026-10931-0
Heng Yin, Chengbi Wang, Kai Zhao, Jianfei Zhao, Liang Chen, Hongfu Zhang, Shanchuan Cao, Jingbo Liu

Developing novel probiotics can help in preventing livestock diarrhea and associated intestinal diseases. Lactic acid bacteria (LAB) are symbiotic intestinal bacteria, which contribute to gastrointestinal tract health. An LAB strain, designated L. johnsonii RS-7, was isolated from the feces of healthy adult pigs and was resistant to acidic conditions and bile salts. In vitro evaluation showed significant antioxidant and anti-inflammatory properties, suggesting its potential application in alleviating intestinal inflammation. An artificially induced colitis model was established in mice to investigate the efficacy of L. johnsonii RS-7. Results indicated that mice administered water containing 3% DSS developed pronounced colitis symptoms, characterized by weight loss, elevated disease activity index, shortened colon length, microvilli shedding, tight junction disruption, reduced goblet cell counts, suppression of anti-inflammatory cytokines, activation of pro-inflammatory cytokines and the TLR4/MyD88/NF-κB signaling pathway, and impaired gut microbiota diversity. These suggest that oral administration of L. johnsonii RS-7 significantly alleviated colitis symptoms. In summary, L. johnsonii RS-7 acted as a probiotic by inhibiting activation of the TLR4/MyD88/NF-κB pathway.

开发新型益生菌有助于预防家畜腹泻及相关肠道疾病。乳酸菌是一种肠道共生菌,对胃肠道健康有重要作用。从健康成年猪的粪便中分离到一株乳酸菌,命名为约翰氏乳杆菌RS-7,该菌株对酸性条件和胆汁盐具有抗性。体外评价显示出显著的抗氧化和抗炎特性,提示其在缓解肠道炎症方面具有潜在的应用前景。建立人工诱导小鼠结肠炎模型,观察约氏乳杆菌RS-7的治疗效果。结果表明,给予含有3% DSS的水的小鼠出现明显的结肠炎症状,其特征是体重减轻、疾病活动指数升高、结肠长度缩短、微绒毛脱落、紧密连接破坏、杯状细胞计数减少、抗炎细胞因子抑制、促炎细胞因子和TLR4/MyD88/NF-κB信号通路激活,以及肠道微生物群多样性受损。这表明口服约氏乳杆菌RS-7可显著缓解结肠炎症状。综上所述,L. johnsonii RS-7通过抑制TLR4/MyD88/NF-κB通路的激活而发挥益生菌的作用。
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引用次数: 0
A Localization-Based Motif Combination Approach To the Design of Antimicrobial Peptides Targeting Gram-Positive and Gram-Negative Bacteria. 基于定位的基序组合方法设计针对革兰氏阳性和革兰氏阴性细菌的抗菌肽
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-01-29 DOI: 10.1007/s12602-025-10875-x
Zülal Kesmen, Saime Gülsüm Batman, Melike Canpolat, Mine Erdem Büyükkiraz

In this study, we aimed to develop a novel antimicrobial peptide (AMP) design strategy via a localization-based motif combination approach to target Gram-negative and Gram-positive pathogenic bacteria. Peptide sequences with high inhibitory activity against Escherichia coli and Staphylococcus aureus were extracted from Database of Antimicrobial Activity and Structure of Peptides (DBAASP) to form two separate datasets for Gram-negative and Gram-positive bacteria, respectively. The peptide sequences in the datasets were segmented into three fragments based on their N-terminal, C-terminal, and central positions, and their statistical significance was scored. The top-scoring fragments were assembled based on their segment-specific positions to generate candidate peptides. The several properties of the candidate peptides were predicted in silico, and the peptides exhibiting the highest antibacterial activity against Gram-positive and Gram-negative bacteria were selected for subsequent synthetic production. The peptides showed membrane deformation effects against the target bacterial strains, and their minimum inhibitory concentration (MIC) values were determined to be between 4 and 16 µg/mL for Gram-negative strains and 4-128 µg/mL for Gram-positive strains. The maximum therapeutic index of the peptides varied between 86 and 129, and their docking scores were found to be higher than known antimicrobial agents, such as nisin and polymyxin B. These results suggest that the designed peptides may serve as effective agents against pathogenic bacteria, including drug-resistant strains, and represent a promising alternative to traditional antibiotics.

在这项研究中,我们旨在通过基于定位的基序组合方法开发一种新的抗菌肽(AMP)设计策略,以靶向革兰氏阴性和革兰氏阳性致病菌。从抗菌肽活性与结构数据库(Database of Antimicrobial activity and Structure of Peptides, DBAASP)中提取对大肠杆菌和金黄色葡萄球菌具有高抑制活性的肽序列,分别形成革兰氏阴性菌和革兰氏阳性菌两个独立的数据集。将数据集中的肽序列根据其n端、c端和中心位置分成3个片段,并对其进行统计显著性评分。根据片段的特定位置组装得分最高的片段以生成候选肽。对候选肽的几种性质进行了硅预测,并选择对革兰氏阳性和革兰氏阴性细菌具有最高抗菌活性的肽进行后续合成生产。结果表明,该肽对目标菌株具有膜变形作用,革兰氏阴性菌株的最小抑制浓度(MIC)为4 ~ 16µg/mL,革兰氏阳性菌株的最小抑制浓度(MIC)为4 ~ 128µg/mL。该多肽的最大治疗指数在86 ~ 129之间,其对接分数高于已知的抗菌药物,如nisin和多粘菌素b。这些结果表明,所设计的多肽可能是一种有效的抗病原菌药物,包括耐药菌株,并代表着传统抗生素的一个有希望的替代方案。
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引用次数: 0
Multi-Omics Analysis Uncovers Acute Hypoxia-Induced Gut Damage and the Underlying Protective Mechanisms of Probiotic Clostridium butyricum B3 in Yellow Catfish (Pelteobagrus fulvidraco). 多组学分析揭示了黄颡鱼急性缺氧诱导的肠道损伤和益生菌丁酸梭菌B3的潜在保护机制
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-01-28 DOI: 10.1007/s12602-025-10908-5
Ziang Qian, Jiale Tian, Qifeng Chen, Yufei Zhang, Cong Zhang, Shaowu Yin, Kai Zhang

Acute hypoxia stress poses a significant challenge in aquaculture, not only compromising gut health but also resulting in substantial economic losses. Using an integrated multi-omics approach, this study demonstrates that hypoxia severely disrupts the intestinal function of yellow catfish (Pelteobagrus fulvidraco), specifically manifesting as phospholipid metabolism disorders, inhibited fatty acid β-oxidation, reduced short-chain fatty acid (SCFA) synthesis, imbalanced gut microbiota (e.g., decreased levels of beneficial lactic acid bacteria Lactococcus and Clostridium sensu stricto 1), and downregulation of detoxification pathways mediated by cytochrome P450. Building upon the previously isolated and identified high-yield SCFA-producing probiotic Clostridium butyricum B3 from yellow catfish in early work, this research further investigated the efficacy and mechanisms of B3 supplementation in mitigating hypoxia-induced intestinal barrier damage in yellow catfish. The results indicated that the supplementation of C. butyricum B3, particularly at a dose of 3.0 × 10⁷ CFU/g, significantly reduced histological damage, enhanced the expression of key tight junction proteins (such as ZO-1 and Claudin), and modulated hypoxia-inducible factor signaling pathways (including HIF-1α, FIH, and PHD). Furthermore, the application of C. butyricum B3 restored microbial ecological balance by promoting the growth of beneficial bacteria like Cetobacterium and inhibiting potential pathogens such as Acinetobacter. In conclusion, these findings underscore the potential of C. butyricum B3 as a novel probiotic strategy for enhancing fish hypoxia tolerance and maintaining intestinal integrity, offering valuable insights for sustainable aquaculture practices.

急性缺氧应激对水产养殖构成重大挑战,不仅损害肠道健康,而且造成重大经济损失。本研究采用综合多组学方法,证明了缺氧严重破坏黄颡鱼(Pelteobagrus fulvidraco)的肠道功能,具体表现为磷脂代谢紊乱,脂肪酸β-氧化抑制,短链脂肪酸(SCFA)合成减少,肠道微生物群失衡(如有益乳酸菌乳球菌和严格感梭菌1水平下降),以及细胞色素P450介导的解毒途径下调。本研究在前期从黄颡鱼中分离并鉴定出高产产scfa益生菌丁酸梭菌B3的基础上,进一步研究了补充B3对黄颡鱼缺氧诱导的肠屏障损伤的影响及其机制。结果表明,添加C. butyricum B3,特别是3.0 × 10⁷CFU/g的剂量,显著降低了组织学损伤,增强了关键紧密连接蛋白(如ZO-1和Claudin)的表达,并调节了缺氧诱导因子信号通路(包括HIF-1α、FIH和PHD)。此外,施用C. butyricum B3可以促进有益菌如Cetobacterium的生长,抑制潜在致病菌如Acinetobacter的生长,从而恢复微生物生态平衡。总之,这些发现强调了丁酸梭菌B3作为增强鱼类缺氧耐受性和维持肠道完整性的新型益生菌策略的潜力,为可持续水产养殖实践提供了有价值的见解。
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引用次数: 0
Probiotic and Microbial Enzymatic Mechanisms for PFAS Detoxification. PFAS解毒的益生菌和微生物酶机制。
IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-01-27 DOI: 10.1007/s12602-026-10921-2
Md Rayhan Chowdhury, Ariful Islam, Valentina Yurina, Takeshi Shimosato

Per- and polyfluoroalkyl substances (PFAS) are highly persistent environmental contaminants that pose a significant threat to ecosystems and human health due to their exceptional chemical stability and resistance to degradation. Conventional remediation methods-such as activated carbon adsorption, ion exchange, and advanced oxidation-primarily transfer PFAS between phases rather than achieving complete mineralization, resulting in the generation of secondary waste. Microbial bioremediation has emerged as a promising, sustainable strategy. Several bacteria, fungi, and cyanobacteria can transform or defluorinate PFAS under environmentally relevant conditions, yielding less fluorinated intermediates. Enzymatic studies have identified oxygenases and reductive dehalogenases as key catalysts in the cleavage of C-F bonds. Moreover, recent evidence indicates that the gut microbiota can adsorb and sequester PFAS, facilitating fecal elimination and reducing systemic toxicity. Advances in synthetic biology now enable the engineering of microbial systems, including probiotic strains, with enhanced PFAS uptake and degradation capabilities. However, significant challenges remain; current microbial pathways primarily act through partial transformation rather than complete mineralization, often accumulating stable fluorinated intermediates. True mineralization is constrained by low enzymatic efficiency, narrow substrate specificity, and the difficulty of translating laboratory success to complex environmental matrices. This review critically synthesizes current progress in microbial and probiotic bioremediation of PFAS, emphasizing enzymatic mechanisms, microbial pathways, and integration with conventional treatment systems. By engaging with these limitations alongside promising advances, we provide a balanced assessment of the feasibility of microbial and engineered probiotic approaches, highlighting knowledge gaps and future directions for developing safe, scalable detoxification technologies.

全氟烷基和多氟烷基物质(PFAS)是高度持久性的环境污染物,由于其特殊的化学稳定性和抗降解性,对生态系统和人类健康构成重大威胁。传统的修复方法,如活性炭吸附、离子交换和高级氧化,主要是将PFAS在相之间转移,而不是完全矿化,导致二次废物的产生。微生物生物修复已经成为一种有前途的、可持续的策略。几种细菌、真菌和蓝藻可以在环境相关条件下转化或去氟化PFAS,产生含氟较少的中间体。酶学研究已经确定了加氧酶和还原脱卤酶是C-F键断裂的关键催化剂。此外,最近的证据表明,肠道微生物群可以吸附和隔离PFAS,促进粪便消除并降低全身毒性。合成生物学的进步使微生物系统的工程,包括益生菌菌株,具有增强的PFAS吸收和降解能力。然而,重大挑战依然存在;目前的微生物途径主要是通过部分转化而不是完全矿化,经常积累稳定的氟化中间体。真正的矿化受到酶效率低、底物特异性窄以及将实验室成功转化为复杂环境基质的困难的限制。本文综述了PFAS微生物和益生菌生物修复的最新进展,重点介绍了酶机制、微生物途径以及与传统处理系统的整合。通过将这些局限性与有希望的进展结合起来,我们对微生物和工程益生菌方法的可行性进行了平衡评估,突出了知识差距和开发安全,可扩展的解毒技术的未来方向。
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引用次数: 0
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Probiotics and Antimicrobial Proteins
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