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DNA destruction under the influence of VUV radiation 紫外线辐射影响下的DNA破坏
Pub Date : 2021-12-02 DOI: 10.1117/12.2613831
G. Zvereva, I. Y. Kirtsidely, E. M. Machs
In the work were investigated the effect of vacuum ultraviolet (VUV) radiation (λ=147 nm, 173 nm) on the DNA of microscopic fungi spores of the species Rhodotorula colostri, Aurobasidium pullulans (colored and uncolored types), Cladosporium herbarum containing different types of protective pigments. The DNA molecules inside the spores (in vivo) and isolated from them (in vitro) were irradiated. The results of electrophoresis showed, that VUV irradiation leads to the appearance of light DNA fragments with sizes 100-750 base pairs, as well as high molecular weight fragments containing more than 20 000 base pairs. The greatest features were observed during the irradiation of Rhodotorula colostri DNA: 1) were formed longer fragments (100-750 base pairs); 2) at λ=172 nm threshold effect was observed: DNA destruction appeared starting from radiation energies of 35 mJ/cm2 during in vivo irradiation and, starting from radiation energies of 20 mJ/cm2, during in vitro irradiation; 2) when irradiated at the wavelength λ=147 nm, the relative destruction of DNA increased, while for other types of the spores it fell. These features can be explained by the presence in Rhodotorula colostri spores of carotene capable of exercising both the properties of the antioxidant and to produce reactive radicals. When irradiated at a wavelength of λ=173 nm in vivo and in vitro, each type of spore had the same characteristic features, which may indicate a small effect on DNA of free water molecules photolysis products. When irradiated at the wavelength λ=147 nm, the influence of melanin was observed: in melanin containing spores the results in vivo and in vitro exposure had the same characteristic features, in the spores without melanin- differed. In the first case, this can be explained by a smaller concentration of reactive radicals due to VUV radiation absorption by melanin, in the second - participation in the DNA destruction of free water molecules photolysis products. A common feature of the electrophoresis results was the presence of long DNA fragments of about 20,000 base pairs in all types of spores. The brightness of these fragments correlated with the DNA destruction degree.
研究了真空紫外(VUV)辐射(λ=147 nm, 173 nm)对含有不同类型保护色素的牛乳红酵母(Rhodotorula colostri)、普鲁兰金丝桃(aurrobasidium pullulans)(有色型和非有色型)和草本枝孢(Cladosporium herbarum)显微真菌孢子DNA的影响。将孢子内(体内)和分离出来的DNA分子(体外)照射。电泳结果表明,VUV照射可产生100-750碱基对大小的轻质DNA片段,以及20,000碱基对以上的高分子量片段。初乳红酵母DNA辐照后的最大特征是:1)形成较长的片段(100-750个碱基对);2)在λ=172 nm处观察到阈值效应:DNA在体内辐照时从35 mJ/cm2的辐射能量开始破坏,在体外辐照时从20 mJ/cm2的辐射能量开始破坏;(2)当波长为λ=147 nm时,DNA的相对破坏增加,而其他类型孢子的相对破坏则下降。这些特征可以用牛初红酵母孢子中存在的胡萝卜素来解释,这些胡萝卜素既能发挥抗氧化剂的作用,又能产生活性自由基。在体内和体外λ=173 nm的波长照射下,每种类型的孢子具有相同的特征特征,这可能表明对游离水分子光解产物DNA的影响很小。当波长λ=147 nm照射时,观察到黑色素的影响:在含黑色素的孢子中,体内和体外暴露的结果具有相同的特征特征,在不含黑色素的孢子中则不同。在第一种情况下,这可以解释为由于VUV辐射被黑色素吸收,活性自由基浓度较小,在第二种情况下,参与DNA破坏的自由水分子光解产物。电泳结果的一个共同特征是在所有类型的孢子中都存在约20,000个碱基对的长DNA片段。这些碎片的亮度与DNA破坏程度相关。
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引用次数: 1
Single wall carbon nanotubes and their conjugates with dimeric phthalocyanine complexes of Cu for optical limiters in the protection of photosensitive detectors and micro-optoelectromechanical systems 单壁碳纳米管及其与铜二聚酞菁配合物的偶联物在光敏探测器和微光电系统保护中的光学限制作用
Pub Date : 2021-12-02 DOI: 10.1117/12.2606048
M. Savelyev, P. Vasilevsky, A. Tolbin, A. Gerasimenko
To protect photosensitive detectors and micro-optoelectromechanical systems, protective equipment is needed against the ingress of powerful laser radiation. Conventional color filters pose a problem for such devices by cutting out the region of the spectrum in which the laser operates. To ensure the possible operation, passive limiters based on nonlinear optical effects can be used. They are capable of transmitting non-hazardous laser radiation with insignificant attenuation (transmission of the order of 70%), and when hit by powerful laser radiation they can quickly darken. The urgency of this problem only increases with the development and widespread use of laser systems. In particular, when using lidars, receiving photosensitive detectors are often damaged. The use of various conjugates with carbon nanotubes is promising for creating limiters. In this work, it is proposed to use conjugates of dimeric phthalocyanine complexes of Cu to create nonlinear optical materials. This material demonstrates the ability to attenuate laser radiation with a nanosecond duration of 16 ns, even in the case of single pulses, due to the synergistic effect of absorption and scattering of radiation. In addition, in the case of 140 fs femtosecond periodic radiation, with a pulse repetition rate of 80 MHz, this material demonstrates the attenuation of radiation when using a cut-off diaphragm due to the effect of self-phase modulation (SSPM). The characteristics of the SSPM pattern were studied, it was noted that the outer rings have the greatest width. In general, the width of the rings gradually increases with distance from the center of the beam. At the same time, the peak fluence (highest fluence value) of each ring is approximately the same, with the exception of the central part. In the central region, a strong effect is observed from heated flows tending upward and thus strongly violating symmetry.
为了保护光敏探测器和微光电系统,需要防护设备来防止强激光辐射的进入。传统的彩色滤光片由于切断了激光工作的光谱区域而给这种装置带来了问题。为了保证可能的运行,可以使用基于非线性光学效应的无源限制器。它们能够传输无害的激光辐射,衰减很小(传输率为70%左右),当受到强大的激光辐射时,它们可以迅速变暗。随着激光系统的发展和广泛使用,这个问题的紧迫性只会增加。特别是在使用激光雷达时,接收光敏探测器往往会损坏。使用碳纳米管的各种共轭物有望产生限制。在这项工作中,提出了使用铜的二聚酞菁配合物的共轭物来创建非线性光学材料。由于辐射的吸收和散射的协同效应,这种材料证明了即使在单脉冲的情况下,也能衰减纳秒持续时间为16 ns的激光辐射。此外,在140秒飞秒周期辐射的情况下,脉冲重复率为80 MHz,该材料证明了由于自相位调制(SSPM)的影响,使用截止膜片时辐射的衰减。研究了SSPM模式的特征,发现外圈宽度最大。一般来说,环的宽度随着离光束中心的距离逐渐增加。同时,除中心部分外,各环的峰值通量(最高通量值)大致相同。在中心区域,观察到一个强烈的影响,从加热流倾向于向上,从而强烈违反对称性。
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引用次数: 0
A smart data control system for environment recognition lidar on Zynq SoCs 基于Zynq soc的环境识别激光雷达智能数据控制系统
Pub Date : 2021-12-02 DOI: 10.1117/12.2613429
Ru-qing Liu, Jing-guo Zhu, Feng Li, Yan Jiang, Cheng-hao Jiang, Zhe Meng, Zhengyu Zhang
Nowadays, laser ranging is widely used in industrial and security fields. As one of the most well-known pulse laser detection system, Lidar is a much effective equipment for environmental recognition. Currently, research on autonomous and smart vehicles technology has been growing exponentially in recent years by integrating more and more smart hardware or software inside. Most studies are almost based on non-real-time operating system with random delays, which leads to lag and inaccuracies in the received data. Based on a self-developed three-dimensional Lidar, a smart embedded system for real-time data transmission on the FPGA Zynq is proposed. The real-time signal acquisition and transmission system is implemented and verified in the three-dimensional imaging experiments system. In this system, verilog code is applied for Lidar data control acquisition and transmission. C/C++ code is used for data calculation and uploading the point cloud data to the computer through the network to realize the real-time display of point cloud. Experimental results demonstrate that the Lidar data are correctly read by the FPGA board. In addition, the real time data acquisition meets the requirements of control and real time data acquisition and procession at 20fps. The proposed approach provides a very useful basic platform for many applications using Lidar as a sensor and will improve their detection accuracy.
目前,激光测距广泛应用于工业和安全领域。激光雷达作为最著名的脉冲激光探测系统之一,是一种非常有效的环境识别设备。目前,自动驾驶和智能汽车技术的研究近年来呈指数级增长,其中集成了越来越多的智能硬件或软件。大多数研究几乎都是基于具有随机延迟的非实时操作系统,这导致接收到的数据存在滞后和不准确。基于自主开发的三维激光雷达,提出了一种基于FPGA Zynq的智能嵌入式实时数据传输系统。该实时信号采集与传输系统在三维成像实验系统中进行了实现和验证。在该系统中,采用verilog码进行激光雷达数据控制的采集和传输。使用C/ c++代码进行数据计算,并通过网络将点云数据上传到计算机,实现点云的实时显示。实验结果表明,FPGA板能够正确读取激光雷达数据。此外,实时数据采集满足控制和20fps实时数据采集和处理的要求。提出的方法为许多使用激光雷达作为传感器的应用提供了一个非常有用的基础平台,并将提高其检测精度。
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引用次数: 0
Light scattering by large irregular ice crystals of cirrus clouds 卷云的大不规则冰晶的光散射
Pub Date : 2021-12-02 DOI: 10.1117/12.2605256
N. Kustova, A. G. Borovoi, A. Konoshonkin, Zhenzhu Wang
The scattering matrixes for randomly oriented large particles of faceted shapes have been calculated using both the geometrical optics and physical optics approximations. We showed that the scattering matrixes are different for regular and irregular particle shapes since the corner reflection effect is predominant at the backward direction for the regular shapes. In the case of irregular shapes, the scattering matrixes have the common feature. Namely, the elements of the matrix reveal near the backward scattering direction some typical peaks that are explained by interference of the backscattered waves. In particular, the negative polarization effect known in astrophysics can be explained by the interference.
利用几何光学近似和物理光学近似计算了随机定向的面形大颗粒的散射矩阵。我们发现,规则和不规则粒子形状的散射矩阵不同,因为规则形状的角反射效应在反向上占主导地位。在不规则形状的情况下,散射矩阵具有共同的特征。即,矩阵的元素在后向散射方向附近显示出一些典型的峰,这些峰是由后向散射波的干涉解释的。特别是,天体物理学中已知的负极化效应可以用干涉来解释。
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引用次数: 0
Features of the xenon excimer VUV spectra formation 氙准分子VUV光谱形成的特征
Pub Date : 2021-12-02 DOI: 10.1117/12.2614089
G. Zvereva
The change in the shape of xenon dimers second continuum spectrum (3,1Σ+u→1Σ+g), excited in a barrier discharge (P = 300 Torr), depending on the gas temperature is studied. It was found that an increase in the gas temperature from Т = 500 K to 1300 K leads to a broadening of the continuum at half maximum from Δλ = 15 nm to Δλ = 20.5 nm and a shift of its maximum to the short-wave region from λ = 173.2 nm to λ = 172.5 nm. The relative increase in intensity occurred in the short-wavelength wing, while the position of the short-wavelength continua boundary did not depend on temperature.The shape of the second continuum of krypton dimers experienced much smaller changes under similar excitation conditions, which was explained by the lower heating of the gas and the lower change in the relative population of the vibrational levels.
研究了在阻挡放电(P = 300 Torr)激发下氙二聚体第二连续谱(3,1Σ+u→1Σ+g)随气体温度的变化。结果表明,当气体温度从Т = 500 K增加到1300 K时,连续谱的半最大值从Δλ = 15 nm扩大到Δλ = 20.5 nm,最大值从λ = 173.2 nm向短波区移动到λ = 172.5 nm。强度的相对增加发生在短波翼,而短波连续边界的位置不依赖于温度。在相似的激发条件下,氪二聚体的第二连续体的形状变化要小得多,这可以解释为气体的加热较低,振动能级的相对居数变化较小。
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引用次数: 0
Formation of the scattering phase function in the interaction of ultrashort laser pulses with a drop and aerosol in a nonlinear mode 超短激光脉冲与液滴和气溶胶非线性相互作用时散射相函数的形成
Pub Date : 2021-12-02 DOI: 10.1117/12.2606961
P. Babushkin, A. Bulygin, E. Danilkin, O. Kuchinskaya, O. Minina, A. V. Petrov
The propagation of ultrashort laser pulses in the atmosphere is accompanied by nonlinear effects. The most low-threshold of them is the effect of cubic nonlinearity along with nonlinear absorption manifesting in aerosol. This effect should lead to the transformation of the scattering phase function formed in a liquid droplet aerosol. To study this effect, numerical and experimental studies on droplets of various sizes and geometries were carried out. As expected, the cubic nonlinearity inclusion should lead to an increase in the effect of backward scattering.
超短激光脉冲在大气中的传播伴随着非线性效应。其中阈值最低的是气溶胶中三次非线性和非线性吸收的影响。这种效应会导致在液滴气溶胶中形成的散射相函数发生转变。为了研究这种影响,对不同尺寸和几何形状的液滴进行了数值和实验研究。正如预期的那样,三次非线性包涵会导致后向散射效应的增加。
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引用次数: 0
Specifics of multiple filamentation formation for femtosecond laser radiation with topological charge 带拓扑电荷的飞秒激光辐射多丝形成的特点
Pub Date : 2021-12-02 DOI: 10.1117/12.2602421
A. Bulygin, A. Zemlyanov
Evolution of local instability in a ring vortex beam propagating in the turbulent atmosphere with highest nonlinearities of the refractive index as well as in uence of the effective characteristics of the light beam is investigated based on numerical calculation of the nonlinear Schrodinger equation with high-order nonlinearities. Propagation of high-power femtosecond laser radiation (HPFLR) with topological charge in the random medium under overlap of an amplitude-phase mask and an original beam is considered. Depending on the mask configuration and initial radiation power, rotation of the beam can lead both to converge and growth more distant of filamentation beginning as well as extension of filamentation area by two and more times.
基于高阶非线性薛定谔方程的数值计算,研究了具有最高折射率非线性的环形涡旋光束在湍流大气中传播时的局部不稳定性演变及其对光束有效特性的影响。研究了带拓扑电荷的高功率飞秒激光在随机介质中,在幅相掩模和原始光束重叠的情况下的传播。根据掩模结构和初始辐射功率的不同,光束的旋转既可以使成丝开始的会聚和增长更远,也可以使成丝面积扩大两倍以上。
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引用次数: 0
Pulse-periodical TEA-CO2 laser with wavelength tuning and pulse energy up to 1.5 J and duration up to 100 ns 波长可调的脉冲周期TEA-CO2激光器,脉冲能量可达1.5 J,持续时间可达100ns
Pub Date : 2021-12-02 DOI: 10.1117/12.2613560
B. Kozlov, D. S. Makhanko, A. Y. Payurov
The conditions for the formation of stable volume discharges at atmospheric pressure in CO2–laser mixtures with an increased content of carbon dioxide and providing the generation of laser radiation pulses in the form of a "giant peak" with a base duration of up to 100 nanoseconds and a radiation energy of up to 1.5 J are determined. With the help of a selective resonator, a discrete rearrangement of radiation on individual vibrational–rotational transitions in the wavelength range of 9.2–10.8 μm is realized. The operation of the TEA–CO2 laser at pulse repetition frequencies up to 50 Hz is ensured.
确定了在大气压下二氧化碳-激光混合物中形成稳定体积放电的条件,增加了二氧化碳含量,并提供了基持续时间高达100纳秒,辐射能量高达1.5 J的“巨峰”形式的激光辐射脉冲的产生。在选择性谐振腔的帮助下,实现了9.2 ~ 10.8 μm波长范围内单个振动-旋转跃迁的离散重排。确保TEA-CO2激光器在高达50 Hz的脉冲重复频率下工作。
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引用次数: 0
Active medium parameters of molecular nitrogen ions in air laser plasma 空气激光等离子体中分子氮离子的活性介质参数
Pub Date : 2021-12-02 DOI: 10.1117/12.2613928
I. A. Zyatikov, V. Losev, V. Prokopev
In this work, self-seeded lasing pulse duration of the В2Σ+u – X2Σ+g transition of molecular nitrogen ion in air laser plasma at different pump pulse focusing conditions was investigated. Pumping was carried out at the central wavelength of 950 nm, with 60 fs duration (FWHM), and 10 mJ energy. It was shown that lasing pulse duration increases from 1.15 to 4.6 ps, and unsaturated gain coefficient decreases from 6.7 to 4.2 with decreasing pump pulse intensity when geometrical focus length was increased. An explanation for picosecond lasing duration at femtosecond seed amplification is proposed.
本文研究了不同泵浦脉冲聚焦条件下空气激光等离子体中分子氮离子В2Σ+u - X2Σ+g跃迁的自种子激光脉冲持续时间。泵送的中心波长为950 nm,持续时间为60 fs,能量为10 mJ。结果表明,当几何焦距增大时,随着泵浦脉冲强度的减小,激光脉冲持续时间从1.15 ps增加到4.6 ps,不饱和增益系数从6.7减小到4.2。提出了飞秒种子放大时皮秒激光持续时间的解释。
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引用次数: 1
Generation mode of runaway electron beams with high amplitude in atmospheric pressure air 常压空气中高振幅失控电子束的产生模式
Pub Date : 2021-12-02 DOI: 10.1117/12.2616494
V. Tarasenko, D. Beloplotov, D. Sorokin, M. Lomaev, E. Baksht, A. Burachenko
The results of experimental studies of various generation modes of runaway electron beams (RAEBs) are presented. The main attention is paid to the mode when the RAEB with the highest amplitude are generated in air at atmospheric pressure. Other modes are discussed too: the most implemented and often mentioned by other authors generation mode as well as the mode when RAEB is generated after breakdown.
本文介绍了各种失控电子束产生模式的实验研究结果。本文主要研究了在大气压力下产生振幅最大的RAEB时的模态。本文还讨论了其他模式,包括实现最多的和其他作者经常提到的生成模式,以及故障后生成RAEB的模式。
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引用次数: 0
期刊
Atomic and Molecular Pulsed Lasers
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