PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations最新文献
Nor Salihah Abdul Manas, Muhd Drus, A. Zulkharnain, J. Hui, N. I. W. Azelee, D. Dailin
Carbazole is a heterocyclic aromatic compound that imposes threat to the environment when contaminates water source. A marine-isolated bacterium, Thalassospira profundimaris shows ability to degrade carbazole. The use of free-cell for bioremediation is inefficient as the cells are exposed to harsh environmental condition. In this study, immobilizations of T. profundimaris in gellan gum were investigated to develop robust systems for bioremediation. The mechanical strength and its relationship with transport of carbazole was investigated. The findings proved that concentration of immobilization media affects diffusivity and mechanical strength. Higher media concentration formed a stronger bead with lower diffusivity where lower concentration formed soft bead with higher diffusivity. The optimum concentration of gellan gum was 0.7% (w/v) with 61% carbazole degradation recorded and an optimum diffusivity of 36.8 × 10−7 cm2/s. It has the highest Young’s modulus (0.041810 N/mm2) among other concentrations. The findings of the optimum carbazole degradation, strength and diffusivity were profound to increase the performance of the bacteria entrapped inside the immobilization media for bioremediation and withstand harsh environment.Carbazole is a heterocyclic aromatic compound that imposes threat to the environment when contaminates water source. A marine-isolated bacterium, Thalassospira profundimaris shows ability to degrade carbazole. The use of free-cell for bioremediation is inefficient as the cells are exposed to harsh environmental condition. In this study, immobilizations of T. profundimaris in gellan gum were investigated to develop robust systems for bioremediation. The mechanical strength and its relationship with transport of carbazole was investigated. The findings proved that concentration of immobilization media affects diffusivity and mechanical strength. Higher media concentration formed a stronger bead with lower diffusivity where lower concentration formed soft bead with higher diffusivity. The optimum concentration of gellan gum was 0.7% (w/v) with 61% carbazole degradation recorded and an optimum diffusivity of 36.8 × 10−7 cm2/s. It has the highest Young’s modulus (0.041810 N/mm2) among other concentrations. The...
{"title":"Transport phenomena of carbazole biodegradation by immobilized Thalasosspira profundimaris cell and mechanical properties","authors":"Nor Salihah Abdul Manas, Muhd Drus, A. Zulkharnain, J. Hui, N. I. W. Azelee, D. Dailin","doi":"10.1063/1.5125513","DOIUrl":"https://doi.org/10.1063/1.5125513","url":null,"abstract":"Carbazole is a heterocyclic aromatic compound that imposes threat to the environment when contaminates water source. A marine-isolated bacterium, Thalassospira profundimaris shows ability to degrade carbazole. The use of free-cell for bioremediation is inefficient as the cells are exposed to harsh environmental condition. In this study, immobilizations of T. profundimaris in gellan gum were investigated to develop robust systems for bioremediation. The mechanical strength and its relationship with transport of carbazole was investigated. The findings proved that concentration of immobilization media affects diffusivity and mechanical strength. Higher media concentration formed a stronger bead with lower diffusivity where lower concentration formed soft bead with higher diffusivity. The optimum concentration of gellan gum was 0.7% (w/v) with 61% carbazole degradation recorded and an optimum diffusivity of 36.8 × 10−7 cm2/s. It has the highest Young’s modulus (0.041810 N/mm2) among other concentrations. The findings of the optimum carbazole degradation, strength and diffusivity were profound to increase the performance of the bacteria entrapped inside the immobilization media for bioremediation and withstand harsh environment.Carbazole is a heterocyclic aromatic compound that imposes threat to the environment when contaminates water source. A marine-isolated bacterium, Thalassospira profundimaris shows ability to degrade carbazole. The use of free-cell for bioremediation is inefficient as the cells are exposed to harsh environmental condition. In this study, immobilizations of T. profundimaris in gellan gum were investigated to develop robust systems for bioremediation. The mechanical strength and its relationship with transport of carbazole was investigated. The findings proved that concentration of immobilization media affects diffusivity and mechanical strength. Higher media concentration formed a stronger bead with lower diffusivity where lower concentration formed soft bead with higher diffusivity. The optimum concentration of gellan gum was 0.7% (w/v) with 61% carbazole degradation recorded and an optimum diffusivity of 36.8 × 10−7 cm2/s. It has the highest Young’s modulus (0.041810 N/mm2) among other concentrations. The...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87912935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sukmaningsih, S. Permana, D. Santjojo, A. Wardoyo, S. Sumitro
This research aimed to prove the ability of extracted freeze-dried java plum fruit applied with a cigarette filter to decrease the intensity of free radicals in the cigarette smoke. It was reported that there was a difference in the spectrum infrared (IR) pattern, leading to a wavelength shift in the smoke burning from a cigarette after the application of the freeze-dried java plum fruit extract on its filter (K1). The XRD analysis indicated that were some diffraction peaks found in K1. This finding suggested that java plum fruit was able to alter the structure and the position of atoms found in the cigarette. In addition, the result of ESR analysis showed that K1 was less paramagnetic than the commercial cigarette smoke (K0). The difference in factor-g value between the K0 and K1 caused an energy splitting, where the commercial cigarette smoke (K0) generated higher factor-g value. The g-factor value was 2.009 and 2.0048, respectively. Furthermore, the ESR spectra indicated a decline in the intensity of free radicals found in K1. Findings of this research, therefore, suggest that the extract of freeze-dried java plum fruit is effective in decreasing the intensity of free radicals in cigarette smoke.This research aimed to prove the ability of extracted freeze-dried java plum fruit applied with a cigarette filter to decrease the intensity of free radicals in the cigarette smoke. It was reported that there was a difference in the spectrum infrared (IR) pattern, leading to a wavelength shift in the smoke burning from a cigarette after the application of the freeze-dried java plum fruit extract on its filter (K1). The XRD analysis indicated that were some diffraction peaks found in K1. This finding suggested that java plum fruit was able to alter the structure and the position of atoms found in the cigarette. In addition, the result of ESR analysis showed that K1 was less paramagnetic than the commercial cigarette smoke (K0). The difference in factor-g value between the K0 and K1 caused an energy splitting, where the commercial cigarette smoke (K0) generated higher factor-g value. The g-factor value was 2.009 and 2.0048, respectively. Furthermore, the ESR spectra indicated a decline in the intensity of f...
{"title":"The potency of java plum (Syzgium cumini) fruit extract as free radical scavenging in cigarette smoke","authors":"A. Sukmaningsih, S. Permana, D. Santjojo, A. Wardoyo, S. Sumitro","doi":"10.1063/1.5125519","DOIUrl":"https://doi.org/10.1063/1.5125519","url":null,"abstract":"This research aimed to prove the ability of extracted freeze-dried java plum fruit applied with a cigarette filter to decrease the intensity of free radicals in the cigarette smoke. It was reported that there was a difference in the spectrum infrared (IR) pattern, leading to a wavelength shift in the smoke burning from a cigarette after the application of the freeze-dried java plum fruit extract on its filter (K1). The XRD analysis indicated that were some diffraction peaks found in K1. This finding suggested that java plum fruit was able to alter the structure and the position of atoms found in the cigarette. In addition, the result of ESR analysis showed that K1 was less paramagnetic than the commercial cigarette smoke (K0). The difference in factor-g value between the K0 and K1 caused an energy splitting, where the commercial cigarette smoke (K0) generated higher factor-g value. The g-factor value was 2.009 and 2.0048, respectively. Furthermore, the ESR spectra indicated a decline in the intensity of free radicals found in K1. Findings of this research, therefore, suggest that the extract of freeze-dried java plum fruit is effective in decreasing the intensity of free radicals in cigarette smoke.This research aimed to prove the ability of extracted freeze-dried java plum fruit applied with a cigarette filter to decrease the intensity of free radicals in the cigarette smoke. It was reported that there was a difference in the spectrum infrared (IR) pattern, leading to a wavelength shift in the smoke burning from a cigarette after the application of the freeze-dried java plum fruit extract on its filter (K1). The XRD analysis indicated that were some diffraction peaks found in K1. This finding suggested that java plum fruit was able to alter the structure and the position of atoms found in the cigarette. In addition, the result of ESR analysis showed that K1 was less paramagnetic than the commercial cigarette smoke (K0). The difference in factor-g value between the K0 and K1 caused an energy splitting, where the commercial cigarette smoke (K0) generated higher factor-g value. The g-factor value was 2.009 and 2.0048, respectively. Furthermore, the ESR spectra indicated a decline in the intensity of f...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86414220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Of late, the number of fire cases in Malaysia has been steadily increasing at a very alarming rate. One way to reduce the ability of fire ignition or spreading is by coating or doping the wood with a layer of fire-protected coating, or an insulating barrier, which is also known as Flame Retardant Coatings (FRCs). In this study, titania (TiO2) is coated onto the surface of the wood to act as an FRC. The synthesis of TiO2 was carried out by using the sol-gel method, while the coating process was done by the dip-coating method. FESEM images show that the surface of the wood has been fully covered by titania nanoparticles (TiO2 NPs) in size range of 24 – 45 nm. From the TGA results, it was shown that the thermal stability of the wood has increased from 300 to 320 °C, with just by a merely coated layer of TiO2 NPs. Flammability testing was carried out by a flame burner also shows that the coated wood is capable of reducing the flammability of the wood, where the coated wood required a longer time to be burned ...
{"title":"Fire-retardancy of wood coated by titania nanoparticles","authors":"Abdul Fatah Deraman, S. Chandren","doi":"10.1063/1.5125526","DOIUrl":"https://doi.org/10.1063/1.5125526","url":null,"abstract":"Of late, the number of fire cases in Malaysia has been steadily increasing at a very alarming rate. One way to reduce the ability of fire ignition or spreading is by coating or doping the wood with a layer of fire-protected coating, or an insulating barrier, which is also known as Flame Retardant Coatings (FRCs). In this study, titania (TiO2) is coated onto the surface of the wood to act as an FRC. The synthesis of TiO2 was carried out by using the sol-gel method, while the coating process was done by the dip-coating method. FESEM images show that the surface of the wood has been fully covered by titania nanoparticles (TiO2 NPs) in size range of 24 – 45 nm. From the TGA results, it was shown that the thermal stability of the wood has increased from 300 to 320 °C, with just by a merely coated layer of TiO2 NPs. Flammability testing was carried out by a flame burner also shows that the coated wood is capable of reducing the flammability of the wood, where the coated wood required a longer time to be burned ...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80402842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Gunam, N. S. Antara, A. Anggreni, Y. Setiyo, I. P. Wiguna, I. Wijaya, I. Putra
The objective of the research was to find out a source of lignocelluloses that could be converted to simple sugars especially glucose as a raw material in bioethanol production. The bioethanol production is an inefficient process, particularly the delignification process. Some solid wastes such as bagasse, corn straw, paddy straw, and sawdust were used as raw materials of lignocelluloses resource. The raw material was dried, ground into small particles, and delignified by using sodium hydroxide (NaOH), ammonia (NH3), and Hydrogen Peroxide (H2O2) in different concentration and soaking time. Two kinds of cellulose resources were chosen based on the value of its potency with the best chemical treatment for delignification. These selected cellulose and chemical used for delignification process were then used as substrate of cellulase enzyme production by Aspergillus niger FNU 6018. Results of the research showed that NaOH was the most effective chemical substance used in delignification process. This solvent also could increase the water retention value (WRV) of the lignocelluloses. Bagasse and corn straw were the potential agriculture waste to be used as raw material in bioethanol production. After delignification process, this raw material contained cellulose, lignin, and WRV of 69.46%, 8.79%, and 8.42, respectively. The FP-ase activity (bagasse as substrate) and CMC-ase activity (corn straw as substrate) of the crude enzyme was 0.0226 U and was 0.0606 U, respectively.The objective of the research was to find out a source of lignocelluloses that could be converted to simple sugars especially glucose as a raw material in bioethanol production. The bioethanol production is an inefficient process, particularly the delignification process. Some solid wastes such as bagasse, corn straw, paddy straw, and sawdust were used as raw materials of lignocelluloses resource. The raw material was dried, ground into small particles, and delignified by using sodium hydroxide (NaOH), ammonia (NH3), and Hydrogen Peroxide (H2O2) in different concentration and soaking time. Two kinds of cellulose resources were chosen based on the value of its potency with the best chemical treatment for delignification. These selected cellulose and chemical used for delignification process were then used as substrate of cellulase enzyme production by Aspergillus niger FNU 6018. Results of the research showed that NaOH was the most effective chemical substance used in delignification process. This solvent ...
{"title":"Chemical pretreatment of lignocellulosic wastes for cellulase production by Aspergillus niger FNU 6018","authors":"I. Gunam, N. S. Antara, A. Anggreni, Y. Setiyo, I. P. Wiguna, I. Wijaya, I. Putra","doi":"10.1063/1.5125544","DOIUrl":"https://doi.org/10.1063/1.5125544","url":null,"abstract":"The objective of the research was to find out a source of lignocelluloses that could be converted to simple sugars especially glucose as a raw material in bioethanol production. The bioethanol production is an inefficient process, particularly the delignification process. Some solid wastes such as bagasse, corn straw, paddy straw, and sawdust were used as raw materials of lignocelluloses resource. The raw material was dried, ground into small particles, and delignified by using sodium hydroxide (NaOH), ammonia (NH3), and Hydrogen Peroxide (H2O2) in different concentration and soaking time. Two kinds of cellulose resources were chosen based on the value of its potency with the best chemical treatment for delignification. These selected cellulose and chemical used for delignification process were then used as substrate of cellulase enzyme production by Aspergillus niger FNU 6018. Results of the research showed that NaOH was the most effective chemical substance used in delignification process. This solvent also could increase the water retention value (WRV) of the lignocelluloses. Bagasse and corn straw were the potential agriculture waste to be used as raw material in bioethanol production. After delignification process, this raw material contained cellulose, lignin, and WRV of 69.46%, 8.79%, and 8.42, respectively. The FP-ase activity (bagasse as substrate) and CMC-ase activity (corn straw as substrate) of the crude enzyme was 0.0226 U and was 0.0606 U, respectively.The objective of the research was to find out a source of lignocelluloses that could be converted to simple sugars especially glucose as a raw material in bioethanol production. The bioethanol production is an inefficient process, particularly the delignification process. Some solid wastes such as bagasse, corn straw, paddy straw, and sawdust were used as raw materials of lignocelluloses resource. The raw material was dried, ground into small particles, and delignified by using sodium hydroxide (NaOH), ammonia (NH3), and Hydrogen Peroxide (H2O2) in different concentration and soaking time. Two kinds of cellulose resources were chosen based on the value of its potency with the best chemical treatment for delignification. These selected cellulose and chemical used for delignification process were then used as substrate of cellulase enzyme production by Aspergillus niger FNU 6018. Results of the research showed that NaOH was the most effective chemical substance used in delignification process. This solvent ...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88153013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. A. Habibah, Y. Anggraito, M. Abdullah, Husni Ahmad Sidiq, Anggi Angeliena, A. Ma’ruf, F. Huyop, A. Retnoningsih
Kundur area in Kepulauan Riau, Indonesia is very well known for having many superior durian cultivars. It is foretold that most of durian plants on the island are originally grown from seeds derived from Malaysian durian. Superiorities of Kundur durian are differently unique in taste, texture and arylus colour. This study aimed to analyse the diversity of superior durian in Kundur using morphological properties and characteristics. Vegetative and generative phases of 30 durian cultivars were observed in five vegetative and seven generative properties. Characteristics that can be observed in each trait were transformed to scores then analysed using the NTSYS-pc 2.02 program to determine the level of similarity/diversity of Kundur durian cultivars. Results showed that similarity of Kundur durian cultivars ranged from 37-78% or had a diversity of 22-63%. There were no cultivars that showed 100% similarity. Based on the results of Pearson’s correlation analysis, 30 of the 48 characteristics observed were positively correlated. Based on fruit characteristics that consumers like, 33.3% Kundur durian is preferred because it has an arylus thickness >10 mm, 80% has a bittersweet taste, 26.7% arylus is dark yellow, and 77% arylus texture is soft and dry.Kundur area in Kepulauan Riau, Indonesia is very well known for having many superior durian cultivars. It is foretold that most of durian plants on the island are originally grown from seeds derived from Malaysian durian. Superiorities of Kundur durian are differently unique in taste, texture and arylus colour. This study aimed to analyse the diversity of superior durian in Kundur using morphological properties and characteristics. Vegetative and generative phases of 30 durian cultivars were observed in five vegetative and seven generative properties. Characteristics that can be observed in each trait were transformed to scores then analysed using the NTSYS-pc 2.02 program to determine the level of similarity/diversity of Kundur durian cultivars. Results showed that similarity of Kundur durian cultivars ranged from 37-78% or had a diversity of 22-63%. There were no cultivars that showed 100% similarity. Based on the results of Pearson’s correlation analysis, 30 of the 48 characteristics observed were posi...
{"title":"Morphological-based diversity analysis of durian from Kundur Island, Indonesia","authors":"N. A. Habibah, Y. Anggraito, M. Abdullah, Husni Ahmad Sidiq, Anggi Angeliena, A. Ma’ruf, F. Huyop, A. Retnoningsih","doi":"10.1063/1.5125532","DOIUrl":"https://doi.org/10.1063/1.5125532","url":null,"abstract":"Kundur area in Kepulauan Riau, Indonesia is very well known for having many superior durian cultivars. It is foretold that most of durian plants on the island are originally grown from seeds derived from Malaysian durian. Superiorities of Kundur durian are differently unique in taste, texture and arylus colour. This study aimed to analyse the diversity of superior durian in Kundur using morphological properties and characteristics. Vegetative and generative phases of 30 durian cultivars were observed in five vegetative and seven generative properties. Characteristics that can be observed in each trait were transformed to scores then analysed using the NTSYS-pc 2.02 program to determine the level of similarity/diversity of Kundur durian cultivars. Results showed that similarity of Kundur durian cultivars ranged from 37-78% or had a diversity of 22-63%. There were no cultivars that showed 100% similarity. Based on the results of Pearson’s correlation analysis, 30 of the 48 characteristics observed were positively correlated. Based on fruit characteristics that consumers like, 33.3% Kundur durian is preferred because it has an arylus thickness >10 mm, 80% has a bittersweet taste, 26.7% arylus is dark yellow, and 77% arylus texture is soft and dry.Kundur area in Kepulauan Riau, Indonesia is very well known for having many superior durian cultivars. It is foretold that most of durian plants on the island are originally grown from seeds derived from Malaysian durian. Superiorities of Kundur durian are differently unique in taste, texture and arylus colour. This study aimed to analyse the diversity of superior durian in Kundur using morphological properties and characteristics. Vegetative and generative phases of 30 durian cultivars were observed in five vegetative and seven generative properties. Characteristics that can be observed in each trait were transformed to scores then analysed using the NTSYS-pc 2.02 program to determine the level of similarity/diversity of Kundur durian cultivars. Results showed that similarity of Kundur durian cultivars ranged from 37-78% or had a diversity of 22-63%. There were no cultivars that showed 100% similarity. Based on the results of Pearson’s correlation analysis, 30 of the 48 characteristics observed were posi...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87526762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ability of Cinnamomum aromaticum essential oil for controlling Tribolium castaneum has good potential to compare with chemical pesticides because of its large secondary metabolites and high volatility. This study aims to determine the effectiveness of Cinnamomum aromaticum compared to chlorpyrifos chemical pesticides for controlling Tribolium castaneum using nanoparticle technology. The research methods consist of preparation, extraction, Cinnamomum aromaticum nanoparticle fabrication, chlorpyrifos chemical pesticides preparation, and Tribolium castaneum mortality test. The experiment was carried out in 24 hours using Cinnamomum aromaticum nanoparticles and chlorpyrifos chemical pesticides with various concentrations, 2%, 4%, 6%, and 8% for each treatment. The results showed that the higher of Cinnamomum aromaticum nanoparticles concentration, the higher the mortality of Tribolium castaneum. The most effective nanoparticle concentration, which has the highest mortality is 8%, with an average of mortality, is 5,331 ± 1,528. In the mortality test using chlorpyrifos, all concentrations lead to 100% mortality after 24 hours. This study concludes that Cinnamomum aromaticum nanoparticle has high potential to control Tribolium castaneum and can reduce the use of chlorpyrifos chemical pesticides which are harmful to humans, animals non-target, and environment.The ability of Cinnamomum aromaticum essential oil for controlling Tribolium castaneum has good potential to compare with chemical pesticides because of its large secondary metabolites and high volatility. This study aims to determine the effectiveness of Cinnamomum aromaticum compared to chlorpyrifos chemical pesticides for controlling Tribolium castaneum using nanoparticle technology. The research methods consist of preparation, extraction, Cinnamomum aromaticum nanoparticle fabrication, chlorpyrifos chemical pesticides preparation, and Tribolium castaneum mortality test. The experiment was carried out in 24 hours using Cinnamomum aromaticum nanoparticles and chlorpyrifos chemical pesticides with various concentrations, 2%, 4%, 6%, and 8% for each treatment. The results showed that the higher of Cinnamomum aromaticum nanoparticles concentration, the higher the mortality of Tribolium castaneum. The most effective nanoparticle concentration, which has the highest mortality is 8%, with an average of mortal...
{"title":"The application of Cinnamomum aromaticum nanoparticle and chlorpyrifos for controlling Tribolium castaneum","authors":"N. Subekti, R. Saputri","doi":"10.1063/1.5125522","DOIUrl":"https://doi.org/10.1063/1.5125522","url":null,"abstract":"The ability of Cinnamomum aromaticum essential oil for controlling Tribolium castaneum has good potential to compare with chemical pesticides because of its large secondary metabolites and high volatility. This study aims to determine the effectiveness of Cinnamomum aromaticum compared to chlorpyrifos chemical pesticides for controlling Tribolium castaneum using nanoparticle technology. The research methods consist of preparation, extraction, Cinnamomum aromaticum nanoparticle fabrication, chlorpyrifos chemical pesticides preparation, and Tribolium castaneum mortality test. The experiment was carried out in 24 hours using Cinnamomum aromaticum nanoparticles and chlorpyrifos chemical pesticides with various concentrations, 2%, 4%, 6%, and 8% for each treatment. The results showed that the higher of Cinnamomum aromaticum nanoparticles concentration, the higher the mortality of Tribolium castaneum. The most effective nanoparticle concentration, which has the highest mortality is 8%, with an average of mortality, is 5,331 ± 1,528. In the mortality test using chlorpyrifos, all concentrations lead to 100% mortality after 24 hours. This study concludes that Cinnamomum aromaticum nanoparticle has high potential to control Tribolium castaneum and can reduce the use of chlorpyrifos chemical pesticides which are harmful to humans, animals non-target, and environment.The ability of Cinnamomum aromaticum essential oil for controlling Tribolium castaneum has good potential to compare with chemical pesticides because of its large secondary metabolites and high volatility. This study aims to determine the effectiveness of Cinnamomum aromaticum compared to chlorpyrifos chemical pesticides for controlling Tribolium castaneum using nanoparticle technology. The research methods consist of preparation, extraction, Cinnamomum aromaticum nanoparticle fabrication, chlorpyrifos chemical pesticides preparation, and Tribolium castaneum mortality test. The experiment was carried out in 24 hours using Cinnamomum aromaticum nanoparticles and chlorpyrifos chemical pesticides with various concentrations, 2%, 4%, 6%, and 8% for each treatment. The results showed that the higher of Cinnamomum aromaticum nanoparticles concentration, the higher the mortality of Tribolium castaneum. The most effective nanoparticle concentration, which has the highest mortality is 8%, with an average of mortal...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91350030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani
CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between s...
{"title":"Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene","authors":"M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani","doi":"10.1063/1.5125545","DOIUrl":"https://doi.org/10.1063/1.5125545","url":null,"abstract":"CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between s...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80493926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anggi Angeliena, A. Ma’ruf, Husni Ahmad Sidiq, Y. Anggraito, N. A. Habibah, F. Huyop, A. Retnoningsih
Indonesia has high diversity of Durian. More than a hundred cultivars have been recognized as superior durians. The main characteristics of superior durian are seen from superiority of taste, texture and color of arillus. Fruit identification and characterization of durian requires a relatively long periods because fruit producing minimum 4 years after planting. Aim of this study was to reveal the diversity of superior Indonesian durians using ISSR molecular markers. DNA samples were collected from 55 superior durian cultivars from five sites in Indonesia (Kundur region in Riau Islands province, Bogor regency in West Java province, Banyuwangi regency in East Java province, Lombok island, and Brongkol Ambarawasub-district in Central Javaprovince). Samples were analyzed using 11 ISSR markers. Amplification of superior durian cultivar DNA produced 83 polymorphic alleles. The highest allele was obtained from the analysis of PKBT 9 markers as many as 13 alleles and the lowest one was taken from the analysis of ISSR 10 markers in 5 alleles. The diversity of 55 superior Indonesian durians using ISSR marker was very high with a polymorphic percentage reached 93.25%. The results of this study indicated that all 55 durian cultivars are different. Similarity analysis showed that none of cultivars had a 100% similarity value.Indonesia has high diversity of Durian. More than a hundred cultivars have been recognized as superior durians. The main characteristics of superior durian are seen from superiority of taste, texture and color of arillus. Fruit identification and characterization of durian requires a relatively long periods because fruit producing minimum 4 years after planting. Aim of this study was to reveal the diversity of superior Indonesian durians using ISSR molecular markers. DNA samples were collected from 55 superior durian cultivars from five sites in Indonesia (Kundur region in Riau Islands province, Bogor regency in West Java province, Banyuwangi regency in East Java province, Lombok island, and Brongkol Ambarawasub-district in Central Javaprovince). Samples were analyzed using 11 ISSR markers. Amplification of superior durian cultivar DNA produced 83 polymorphic alleles. The highest allele was obtained from the analysis of PKBT 9 markers as many as 13 alleles and the lowest one was taken from the analysis of...
{"title":"The diversity of superior Indonesian durians based on molecular markers","authors":"Anggi Angeliena, A. Ma’ruf, Husni Ahmad Sidiq, Y. Anggraito, N. A. Habibah, F. Huyop, A. Retnoningsih","doi":"10.1063/1.5125547","DOIUrl":"https://doi.org/10.1063/1.5125547","url":null,"abstract":"Indonesia has high diversity of Durian. More than a hundred cultivars have been recognized as superior durians. The main characteristics of superior durian are seen from superiority of taste, texture and color of arillus. Fruit identification and characterization of durian requires a relatively long periods because fruit producing minimum 4 years after planting. Aim of this study was to reveal the diversity of superior Indonesian durians using ISSR molecular markers. DNA samples were collected from 55 superior durian cultivars from five sites in Indonesia (Kundur region in Riau Islands province, Bogor regency in West Java province, Banyuwangi regency in East Java province, Lombok island, and Brongkol Ambarawasub-district in Central Javaprovince). Samples were analyzed using 11 ISSR markers. Amplification of superior durian cultivar DNA produced 83 polymorphic alleles. The highest allele was obtained from the analysis of PKBT 9 markers as many as 13 alleles and the lowest one was taken from the analysis of ISSR 10 markers in 5 alleles. The diversity of 55 superior Indonesian durians using ISSR marker was very high with a polymorphic percentage reached 93.25%. The results of this study indicated that all 55 durian cultivars are different. Similarity analysis showed that none of cultivars had a 100% similarity value.Indonesia has high diversity of Durian. More than a hundred cultivars have been recognized as superior durians. The main characteristics of superior durian are seen from superiority of taste, texture and color of arillus. Fruit identification and characterization of durian requires a relatively long periods because fruit producing minimum 4 years after planting. Aim of this study was to reveal the diversity of superior Indonesian durians using ISSR molecular markers. DNA samples were collected from 55 superior durian cultivars from five sites in Indonesia (Kundur region in Riau Islands province, Bogor regency in West Java province, Banyuwangi regency in East Java province, Lombok island, and Brongkol Ambarawasub-district in Central Javaprovince). Samples were analyzed using 11 ISSR markers. Amplification of superior durian cultivar DNA produced 83 polymorphic alleles. The highest allele was obtained from the analysis of PKBT 9 markers as many as 13 alleles and the lowest one was taken from the analysis of...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77339334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Omar, S. Hashim, S. K. Ghoshal, N. D. Shariff, A. Hashim
For single point dose measurements, the use of optically stimulated luminescence dosimeters (OSLDs), nanoDots is gaining a remarkable interest due to availability and potential attributes. In this ...
{"title":"Light induced fading in optically stimulated luminescence dots for medical dosimetry measurement","authors":"R. Omar, S. Hashim, S. K. Ghoshal, N. D. Shariff, A. Hashim","doi":"10.1063/1.5125516","DOIUrl":"https://doi.org/10.1063/1.5125516","url":null,"abstract":"For single point dose measurements, the use of optically stimulated luminescence dosimeters (OSLDs), nanoDots is gaining a remarkable interest due to availability and potential attributes. In this ...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90077755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Mahat, Kalaiarasi Dhevaseelan, Nor’ Ashikin Sharif, Hussein Omar Khan, A. Azman, Elizabeth Layang, Siti Afifah Ismail
Dental remains one of the most reliable forensic means for identification, especially when facial recognition and fingerprints are inconclusive. Even though previous studies reported the possibilit...
{"title":"Recovery of human DNA from canine teeth exposed to direct heating of 300 °C at varying durations for forensic identification","authors":"N. Mahat, Kalaiarasi Dhevaseelan, Nor’ Ashikin Sharif, Hussein Omar Khan, A. Azman, Elizabeth Layang, Siti Afifah Ismail","doi":"10.1063/1.5125508","DOIUrl":"https://doi.org/10.1063/1.5125508","url":null,"abstract":"Dental remains one of the most reliable forensic means for identification, especially when facial recognition and fingerprints are inconclusive. Even though previous studies reported the possibilit...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79201153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations
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