PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations最新文献
Cancer is characterized by cell growth uncontrolled in tissue and doesn’t recognize apoptosis. Cheral® is an herbal medicinal product that contains meniran (Phyllanthus niruri) and turmeric (Curcum...
{"title":"Exploring of Cheral® potential as anticancer agent via apoptosis mechanism","authors":"Sapti Puspitarini, Widodo, N. Lestari, M. Rifa’i","doi":"10.1063/1.5125553","DOIUrl":"https://doi.org/10.1063/1.5125553","url":null,"abstract":"Cancer is characterized by cell growth uncontrolled in tissue and doesn’t recognize apoptosis. Cheral® is an herbal medicinal product that contains meniran (Phyllanthus niruri) and turmeric (Curcum...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74908591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sambucus ebulus L. is used in local folk medicine to treat several diseases in Europe and West Asia. The objective of the review is to evaluate the S. ebulus L. in terms of past, current and future perspective. Various parts of the plant have been long used in traditional medicine for food, medicinal and ornamental purpose. Until recently, however, substantial scientific research concerning its properties has been lacking. Here, we evaluated the state of past and current scientific research concerning the use of the plant extract and related products, particularly in the treatment of antimicrobial activity. S. ebulus L. has a very high invitro antioxidant activity and no mutagenic effects at low concentrations, which makes it recommendable for applications in the food industry and pharmacology. Future studies will focus on the use of S. ebulus L. in the food and pharmaceutical industry.Sambucus ebulus L. is used in local folk medicine to treat several diseases in Europe and West Asia. The objective of the review is to evaluate the S. ebulus L. in terms of past, current and future perspective. Various parts of the plant have been long used in traditional medicine for food, medicinal and ornamental purpose. Until recently, however, substantial scientific research concerning its properties has been lacking. Here, we evaluated the state of past and current scientific research concerning the use of the plant extract and related products, particularly in the treatment of antimicrobial activity. S. ebulus L. has a very high invitro antioxidant activity and no mutagenic effects at low concentrations, which makes it recommendable for applications in the food industry and pharmacology. Future studies will focus on the use of S. ebulus L. in the food and pharmaceutical industry.
{"title":"Sambucus ebulus L.: Past, present and future","authors":"Y. Kaya, E. Haji, Y. E. Arvas, H. Aksoy","doi":"10.1063/1.5125534","DOIUrl":"https://doi.org/10.1063/1.5125534","url":null,"abstract":"Sambucus ebulus L. is used in local folk medicine to treat several diseases in Europe and West Asia. The objective of the review is to evaluate the S. ebulus L. in terms of past, current and future perspective. Various parts of the plant have been long used in traditional medicine for food, medicinal and ornamental purpose. Until recently, however, substantial scientific research concerning its properties has been lacking. Here, we evaluated the state of past and current scientific research concerning the use of the plant extract and related products, particularly in the treatment of antimicrobial activity. S. ebulus L. has a very high invitro antioxidant activity and no mutagenic effects at low concentrations, which makes it recommendable for applications in the food industry and pharmacology. Future studies will focus on the use of S. ebulus L. in the food and pharmaceutical industry.Sambucus ebulus L. is used in local folk medicine to treat several diseases in Europe and West Asia. The objective of the review is to evaluate the S. ebulus L. in terms of past, current and future perspective. Various parts of the plant have been long used in traditional medicine for food, medicinal and ornamental purpose. Until recently, however, substantial scientific research concerning its properties has been lacking. Here, we evaluated the state of past and current scientific research concerning the use of the plant extract and related products, particularly in the treatment of antimicrobial activity. S. ebulus L. has a very high invitro antioxidant activity and no mutagenic effects at low concentrations, which makes it recommendable for applications in the food industry and pharmacology. Future studies will focus on the use of S. ebulus L. in the food and pharmaceutical industry.","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80182183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andriana Joanes Leong, Ng Nyuk-Ting, Nurafifah Mohd Nor, U. Baig, W. Ibrahim, M. M. Sanagi, A. S. A. Keyon
In this work, cellulose acetate-(3-aminopropyl)triethoxysilane (CA-APTES) was evaluated for the first time as adsorbent for the removal of rhodamine 6G (R6G) and crystal violet (CV) in water. The sorbent was first synthesized then characterized using attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). The presence of C=O amide at 1740.17 cm−1 and N-H amide around 3451.98 cm−1 confirmed that the sorbent has been successfully synthesized. The removal efficiency was determined using ultraviolet-visible (UV-Vis) spectrophotometry, where the optimum conditions were found to be at 2 ppm initial dyes concentration, sample solution of pH 7, 50 mg of sorbent, and 2 hours of shaking time. Kinetic studies for the sorbents were carried out using both pseudo-first-order and pseudo-second-order models, where the R2 values for removal R6G and CV were 0.0064 and 0.0523 respectively for the former, and R2 0.75 and 0.87 respectively for the latter. The kinetic study was best described using pseudo-second-order model. The optimized method was adopted for the dyes removal in real batik textile wastewater, whereby removal efficiencies of 61.81 % for CV and 48.62 % for R6G were obtained.In this work, cellulose acetate-(3-aminopropyl)triethoxysilane (CA-APTES) was evaluated for the first time as adsorbent for the removal of rhodamine 6G (R6G) and crystal violet (CV) in water. The sorbent was first synthesized then characterized using attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). The presence of C=O amide at 1740.17 cm−1 and N-H amide around 3451.98 cm−1 confirmed that the sorbent has been successfully synthesized. The removal efficiency was determined using ultraviolet-visible (UV-Vis) spectrophotometry, where the optimum conditions were found to be at 2 ppm initial dyes concentration, sample solution of pH 7, 50 mg of sorbent, and 2 hours of shaking time. Kinetic studies for the sorbents were carried out using both pseudo-first-order and pseudo-second-order models, where the R2 values for removal R6G and CV were 0.0064 and 0.0523 respectively for the former, and R2 0.75 and 0.87 respectively for the latter. The kinetic study was best described using pseu...
{"title":"Removal of rhodamine 6G and crystal violet dyes from water sample using cellulose acetate-(3-aminopropyl)-triethoxysilane sorbent","authors":"Andriana Joanes Leong, Ng Nyuk-Ting, Nurafifah Mohd Nor, U. Baig, W. Ibrahim, M. M. Sanagi, A. S. A. Keyon","doi":"10.1063/1.5125517","DOIUrl":"https://doi.org/10.1063/1.5125517","url":null,"abstract":"In this work, cellulose acetate-(3-aminopropyl)triethoxysilane (CA-APTES) was evaluated for the first time as adsorbent for the removal of rhodamine 6G (R6G) and crystal violet (CV) in water. The sorbent was first synthesized then characterized using attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). The presence of C=O amide at 1740.17 cm−1 and N-H amide around 3451.98 cm−1 confirmed that the sorbent has been successfully synthesized. The removal efficiency was determined using ultraviolet-visible (UV-Vis) spectrophotometry, where the optimum conditions were found to be at 2 ppm initial dyes concentration, sample solution of pH 7, 50 mg of sorbent, and 2 hours of shaking time. Kinetic studies for the sorbents were carried out using both pseudo-first-order and pseudo-second-order models, where the R2 values for removal R6G and CV were 0.0064 and 0.0523 respectively for the former, and R2 0.75 and 0.87 respectively for the latter. The kinetic study was best described using pseudo-second-order model. The optimized method was adopted for the dyes removal in real batik textile wastewater, whereby removal efficiencies of 61.81 % for CV and 48.62 % for R6G were obtained.In this work, cellulose acetate-(3-aminopropyl)triethoxysilane (CA-APTES) was evaluated for the first time as adsorbent for the removal of rhodamine 6G (R6G) and crystal violet (CV) in water. The sorbent was first synthesized then characterized using attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). The presence of C=O amide at 1740.17 cm−1 and N-H amide around 3451.98 cm−1 confirmed that the sorbent has been successfully synthesized. The removal efficiency was determined using ultraviolet-visible (UV-Vis) spectrophotometry, where the optimum conditions were found to be at 2 ppm initial dyes concentration, sample solution of pH 7, 50 mg of sorbent, and 2 hours of shaking time. Kinetic studies for the sorbents were carried out using both pseudo-first-order and pseudo-second-order models, where the R2 values for removal R6G and CV were 0.0064 and 0.0523 respectively for the former, and R2 0.75 and 0.87 respectively for the latter. The kinetic study was best described using pseu...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85229737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual
Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...
菖蒲是一种未被充分利用的植物，它含有生物活性化合物，如酚类物质、类黄酮和萜类。酚类化合物的积累在植物的各个部位是不同的;因此，本实验采用不同的有机溶剂从叶和根茎中提取酚类化合物。采用2,2-二苯基-1-苦味酰肼(DPPH)法测定其抗氧化活性。结果表明，甲醇、丙酮、乙酸乙酯和己烷提取的总酚含量分别为22.17 mg GAE/g、15.89 mg GAE/g、12.79 mg GAE/g和11.49 mg GAE/g。按槲皮素标准计算，黄酮类含量分别为5.07 mg QE /g、6.55 mg QE /g、3.85 mg QE /g和2.23 mg QE /g。另一方面，各有机溶剂提取的根总酚和类黄酮含量分别为240.32 mg GAE/g、139.02 mg GAE/g、99.63 mg GAE/g、105.57 mg GAE/g和63.06 mg QE /g、48.35 mg QE /g、43.07 mg QE /g、31.78 mg QE /g。叶片和根茎提取物的抗氧化活性表现为清除自由基的活性在75% ~ 90%之间。GC-MS检测到的主要挥发性化合物为α-和β-细辛酮。菖蒲是一种未被充分利用的植物，它含有生物活性化合物，如酚类物质、类黄酮和萜类。酚类化合物的积累在植物的各个部位是不同的;因此，本实验采用不同的有机溶剂从叶和根茎中提取酚类化合物。采用2,2-二苯基-1-苦味酰肼(DPPH)法测定其抗氧化活性。结果表明，甲醇、丙酮、乙酸乙酯和己烷提取的总酚含量分别为22.17 mg GAE/g、15.89 mg GAE/g、12.79 mg GAE/g和11.49 mg GAE/g。按槲皮素标准计算，黄酮类含量分别为5.07 mg QE /g、6.55 mg QE /g、3.85 mg QE /g和2.23 mg QE /g。另一方面，各有机溶剂提取的木耳根总酚和总黄酮含量分别为240.32 mg GAE/g、139.02 mg GAE/g、99.63 mg GAE/g、105.57 mg GAE/g和63.06 mg QE /g、48.35 mg QE /g、43.07 mg QE /g、31.78 mg QE /g、…
{"title":"Antioxidant activity of karimenga (Acorus calamus)","authors":"G. S. Djarkasi, Lana E. Lalujan, Erny J. N. Nurali, M. Sumual","doi":"10.1063/1.5125555","DOIUrl":"https://doi.org/10.1063/1.5125555","url":null,"abstract":"Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g, respectively. The antioxidant activities of leaf and rhizome extracts expressed as free radical scavenging activity were in the range of 75 – 90 %. The main volatile compound detected by GC-MS was α- and β-asarone.Karimenga (Acorus calamus) is an underutilized plant that contains bioactive compounds such as phenolics, flavonoids, and terpenoids. The accumulation of phenolic compounds varies in each part of the plant; hence the phenolic compounds in this experiment were extracted from leaf and rhizome using different organic solvents. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that total phenol from the leaf extracted by methanol, acetone, ethyl acetate, and hexane were 22.17 mg GAE/g, 15.89 mg GAE /g, 12.79 mg GAE /g, and 11.49 mg GAE/g respectively. The flavonoid content were 5.07 mg QE /g, 6.55 mg QE /g, 3.85 mg QE /g, and 2.23 mg QE /g respectively which were calculated base on quercetin standard. On the other hand, the total phenol and flavonoid content from karimenga rhizome extracted by each organic solvent were 240.32 mg GAE/g, 139.02 mg GAE/g, 99.63 mg GAE/g, 105.57 mg GAE/g, and 63.06 mg QE /g, 48.35 mg QE /g, 43.07 mg QE /g, 31.78 mg QE /g,...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84603335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The utilization of low value agro-industrial waste as valuable end product has become a key research priority in the recent years. Currently, the commercial cultivation of Pleurotus ostreatus using sawdust from rubber tree as the base substrate in Malaysia has been reported. However, the price and demand of the rubber tree sawdust (RS) have been increasing, affecting the overall production cost. Thus, a new inexpensive alternative substrate yet as effective as rubber tree sawdust is needed to replace the RS as the base substrate for the cultivation of P. ostreatus. In this study, the investigation on substrates from various agro-industrial waste in Malaysia was carried out. Nine different substrates were prepared in combinations in the ratio of 0.25 w/w and 0.5 w/w with empty fruit bunch (EFB), palm pressed fibre (PPF), sugarcane bagasse (SGB) and RS as an alternative substrate for the cultivation of P. ostreatus. These substrates were supplemented with fixed ratio of wheat bran and calcium carbonate to increase the yield of P. ostreatus. The number of days mycelium growth, yield (g/kg substrates), BE (%) and nutrient compositions were studied for different the nine combinations of EFB, PPF and SGB to RS. The substrate of 25%PPF + 25%SGB + 50%RS was found as the best formulation in term of growth performance, yield and biological efficiency (BE) (%) with 35 days for first harvest at 318.88 g/kg and 79.72%, respectively. Thus, the combination of EFB, PPF and SGB demonstrated a great potential as an alternative substrate for the cultivation of P. ostreatus when incorporated with RS.The utilization of low value agro-industrial waste as valuable end product has become a key research priority in the recent years. Currently, the commercial cultivation of Pleurotus ostreatus using sawdust from rubber tree as the base substrate in Malaysia has been reported. However, the price and demand of the rubber tree sawdust (RS) have been increasing, affecting the overall production cost. Thus, a new inexpensive alternative substrate yet as effective as rubber tree sawdust is needed to replace the RS as the base substrate for the cultivation of P. ostreatus. In this study, the investigation on substrates from various agro-industrial waste in Malaysia was carried out. Nine different substrates were prepared in combinations in the ratio of 0.25 w/w and 0.5 w/w with empty fruit bunch (EFB), palm pressed fibre (PPF), sugarcane bagasse (SGB) and RS as an alternative substrate for the cultivation of P. ostreatus. These substrates were supplemented with fixed ratio of wheat bran and calcium carbonate to i...
{"title":"Growth and yield performance of Pleurotus ostreatus on various agro-industrial wastes in Malaysia","authors":"F. A. Zakil, M. Sueb, R. Isha","doi":"10.1063/1.5125559","DOIUrl":"https://doi.org/10.1063/1.5125559","url":null,"abstract":"The utilization of low value agro-industrial waste as valuable end product has become a key research priority in the recent years. Currently, the commercial cultivation of Pleurotus ostreatus using sawdust from rubber tree as the base substrate in Malaysia has been reported. However, the price and demand of the rubber tree sawdust (RS) have been increasing, affecting the overall production cost. Thus, a new inexpensive alternative substrate yet as effective as rubber tree sawdust is needed to replace the RS as the base substrate for the cultivation of P. ostreatus. In this study, the investigation on substrates from various agro-industrial waste in Malaysia was carried out. Nine different substrates were prepared in combinations in the ratio of 0.25 w/w and 0.5 w/w with empty fruit bunch (EFB), palm pressed fibre (PPF), sugarcane bagasse (SGB) and RS as an alternative substrate for the cultivation of P. ostreatus. These substrates were supplemented with fixed ratio of wheat bran and calcium carbonate to increase the yield of P. ostreatus. The number of days mycelium growth, yield (g/kg substrates), BE (%) and nutrient compositions were studied for different the nine combinations of EFB, PPF and SGB to RS. The substrate of 25%PPF + 25%SGB + 50%RS was found as the best formulation in term of growth performance, yield and biological efficiency (BE) (%) with 35 days for first harvest at 318.88 g/kg and 79.72%, respectively. Thus, the combination of EFB, PPF and SGB demonstrated a great potential as an alternative substrate for the cultivation of P. ostreatus when incorporated with RS.The utilization of low value agro-industrial waste as valuable end product has become a key research priority in the recent years. Currently, the commercial cultivation of Pleurotus ostreatus using sawdust from rubber tree as the base substrate in Malaysia has been reported. However, the price and demand of the rubber tree sawdust (RS) have been increasing, affecting the overall production cost. Thus, a new inexpensive alternative substrate yet as effective as rubber tree sawdust is needed to replace the RS as the base substrate for the cultivation of P. ostreatus. In this study, the investigation on substrates from various agro-industrial waste in Malaysia was carried out. Nine different substrates were prepared in combinations in the ratio of 0.25 w/w and 0.5 w/w with empty fruit bunch (EFB), palm pressed fibre (PPF), sugarcane bagasse (SGB) and RS as an alternative substrate for the cultivation of P. ostreatus. These substrates were supplemented with fixed ratio of wheat bran and calcium carbonate to i...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73878189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laser-induced breakdown spectroscopy (LIBS) has been applied to perform elemental analysis of printing ink samples. Samples of black printing inks from three types of printers viz. inkjet, laser-jet, and photocopier (three different brands for each type) and one control sample (blank white A4 paper) were analysed under optimised conditions. Results revealed that the LIBS method when coupled with PCA was able to provide discriminative evidence on elemental differences among all the different printing inks. Considering its time and cost effectiveness as well as requiring only minute amount of sample with no sample pre-treatment steps, the combination of LIBS and PCA may prove useful for forensic questioned document practical caseworks.Laser-induced breakdown spectroscopy (LIBS) has been applied to perform elemental analysis of printing ink samples. Samples of black printing inks from three types of printers viz. inkjet, laser-jet, and photocopier (three different brands for each type) and one control sample (blank white A4 paper) were analysed under optimised conditions. Results revealed that the LIBS method when coupled with PCA was able to provide discriminative evidence on elemental differences among all the different printing inks. Considering its time and cost effectiveness as well as requiring only minute amount of sample with no sample pre-treatment steps, the combination of LIBS and PCA may prove useful for forensic questioned document practical caseworks.
{"title":"Laser-induced breakdown spectroscopy (LIBS) for printing ink analysis coupled with principle component analysis (PCA)","authors":"Y. W. Hui, N. Mahat, D. Ismail, R. R. Ibrahim","doi":"10.1063/1.5125514","DOIUrl":"https://doi.org/10.1063/1.5125514","url":null,"abstract":"Laser-induced breakdown spectroscopy (LIBS) has been applied to perform elemental analysis of printing ink samples. Samples of black printing inks from three types of printers viz. inkjet, laser-jet, and photocopier (three different brands for each type) and one control sample (blank white A4 paper) were analysed under optimised conditions. Results revealed that the LIBS method when coupled with PCA was able to provide discriminative evidence on elemental differences among all the different printing inks. Considering its time and cost effectiveness as well as requiring only minute amount of sample with no sample pre-treatment steps, the combination of LIBS and PCA may prove useful for forensic questioned document practical caseworks.Laser-induced breakdown spectroscopy (LIBS) has been applied to perform elemental analysis of printing ink samples. Samples of black printing inks from three types of printers viz. inkjet, laser-jet, and photocopier (three different brands for each type) and one control sample (blank white A4 paper) were analysed under optimised conditions. Results revealed that the LIBS method when coupled with PCA was able to provide discriminative evidence on elemental differences among all the different printing inks. Considering its time and cost effectiveness as well as requiring only minute amount of sample with no sample pre-treatment steps, the combination of LIBS and PCA may prove useful for forensic questioned document practical caseworks.","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75662932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Abed, T. A. Farhan, A. A. Kadhum, M. Abed, Noor A. Zaki, M. F. Edbeib
The aim of this study is to evaluate the efficacy of some biological control agents in the resistance of Trichoderma harzianium, Trichoderma viride and mycorrhizal fungi Glomus mosseae, individually or collectively, Fusarim oxysporum f.sp. cucumerinum. The results of this study showed that the combined biological control factors had a positive and significant effect in reducing the severity of injury of the disease by 20% and 21% respectively, with an effect efficiency of 73.92% and 65.30% in reducing the percent of the injury severity. Followed by the treatment of mycorrhizal fungi G. mosseae, the results showed a reduction of injury severity with 26.7%, 23% and 65.18%, 62.47%. The use of T.harzianium and T. viride has also resulted in a significant reduction of the injury severity by 43.3%, 23%, 46.7%, 29.3%, 46.7% and 23%, respectively. In addition, the use of these biological control agents had a significant impact on the growth. An increase in the plant height, leaves number, wet and dry weight of plant was also observed.The aim of this study is to evaluate the efficacy of some biological control agents in the resistance of Trichoderma harzianium, Trichoderma viride and mycorrhizal fungi Glomus mosseae, individually or collectively, Fusarim oxysporum f.sp. cucumerinum. The results of this study showed that the combined biological control factors had a positive and significant effect in reducing the severity of injury of the disease by 20% and 21% respectively, with an effect efficiency of 73.92% and 65.30% in reducing the percent of the injury severity. Followed by the treatment of mycorrhizal fungi G. mosseae, the results showed a reduction of injury severity with 26.7%, 23% and 65.18%, 62.47%. The use of T.harzianium and T. viride has also resulted in a significant reduction of the injury severity by 43.3%, 23%, 46.7%, 29.3%, 46.7% and 23%, respectively. In addition, the use of these biological control agents had a significant impact on the growth. An increase in the plant height, leaves number, wet and dry weight of pl...
{"title":"Effect of some biocontrol factors and their efficacy in resistance to Fusarium wilt disease caused by Fusarium oxysporum f.sp. cucumerinum on cucumber plant under open field conditions","authors":"J. Abed, T. A. Farhan, A. A. Kadhum, M. Abed, Noor A. Zaki, M. F. Edbeib","doi":"10.1063/1.5125535","DOIUrl":"https://doi.org/10.1063/1.5125535","url":null,"abstract":"The aim of this study is to evaluate the efficacy of some biological control agents in the resistance of Trichoderma harzianium, Trichoderma viride and mycorrhizal fungi Glomus mosseae, individually or collectively, Fusarim oxysporum f.sp. cucumerinum. The results of this study showed that the combined biological control factors had a positive and significant effect in reducing the severity of injury of the disease by 20% and 21% respectively, with an effect efficiency of 73.92% and 65.30% in reducing the percent of the injury severity. Followed by the treatment of mycorrhizal fungi G. mosseae, the results showed a reduction of injury severity with 26.7%, 23% and 65.18%, 62.47%. The use of T.harzianium and T. viride has also resulted in a significant reduction of the injury severity by 43.3%, 23%, 46.7%, 29.3%, 46.7% and 23%, respectively. In addition, the use of these biological control agents had a significant impact on the growth. An increase in the plant height, leaves number, wet and dry weight of plant was also observed.The aim of this study is to evaluate the efficacy of some biological control agents in the resistance of Trichoderma harzianium, Trichoderma viride and mycorrhizal fungi Glomus mosseae, individually or collectively, Fusarim oxysporum f.sp. cucumerinum. The results of this study showed that the combined biological control factors had a positive and significant effect in reducing the severity of injury of the disease by 20% and 21% respectively, with an effect efficiency of 73.92% and 65.30% in reducing the percent of the injury severity. Followed by the treatment of mycorrhizal fungi G. mosseae, the results showed a reduction of injury severity with 26.7%, 23% and 65.18%, 62.47%. The use of T.harzianium and T. viride has also resulted in a significant reduction of the injury severity by 43.3%, 23%, 46.7%, 29.3%, 46.7% and 23%, respectively. In addition, the use of these biological control agents had a significant impact on the growth. An increase in the plant height, leaves number, wet and dry weight of pl...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74960856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Lestari, Suherr Khalleefah, Sapti Puspitarini, Widodo, A. Soewondo, Muhamin Rifa’I
Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Hyperglycemia in DM leads to bone marrow dysfunction in the hematopoiesis process of hematopoietic stem cells (HSC). Th...
{"title":"The role of Moringa oliefera-Albumin combination as a traditional medicine to control the development of SDF-1 and TER-119+VLA-4+ in diabetes mice models","authors":"N. Lestari, Suherr Khalleefah, Sapti Puspitarini, Widodo, A. Soewondo, Muhamin Rifa’I","doi":"10.1063/1.5125552","DOIUrl":"https://doi.org/10.1063/1.5125552","url":null,"abstract":"Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia. Hyperglycemia in DM leads to bone marrow dysfunction in the hematopoiesis process of hematopoietic stem cells (HSC). Th...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74807592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani
Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.
{"title":"Establishment of primary 3D cell culture based on magnetic bioprinting for colorectal cancer cells from patients in Cipto Mangunkusumo National Hospital Indonesia","authors":"M. Abdullah, B. Bela, A. Syam, M. Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, D. Makmun, A. A. Rani","doi":"10.1063/1.5125529","DOIUrl":"https://doi.org/10.1063/1.5125529","url":null,"abstract":"Colorectal cancer is the third most common malignancy with more than 1.2 million new annual cases in the world. Methods for propagating cancer cells range from conventional to the more advanced system. Cancer cells are routinely cultured in two-dimensional (2D) model. This method faces several limitations attributable to lack of structural architecture and nature condition. Three-dimensional (3D) model mimics in vivo environment more closely than the conventional 2D. This strategy may help in elucidating cancer biology and subsequently contribute to higher success of therapy. The goal of this study was to establish the primary 3D culture from colorectal cancer collected from patients in Cipto Mangunkusumo National Hospital. Cancer cells were extracted mechanically and grown in DMEM/F12 medium supplemented with serum and antibiotics. The NanoShuttle-PL was added to the cells and magnetic drive was used to form cell spheroids. There were 22 samples obtained with varying cancer stages viz. IIA, IIIB, IIIC, and IV. Primary culture of cancer cells successfully established by mechanically extracting the tumour tissues into small pieces. Results on magnetic bioprinting revealed that the 3D culture (spheroids) was successfully established rapidly. Further studies are needed to evaluate culture viability and cell compositions, as well as performing the chemosensitivity testing.","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82650940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nurhaziqah Supari, Y. Kaya, Maral Biroudian, M. Javed
Exploring the diversity at the molecular level is one of the prerequisites in modern plant breeding to address the world food security challenges. Malaysian rice cultivars have been characterized using single sequence repeat (SSR) markers to assess the molecular diversity. In this study, 21 Malaysian rice cultivars were characterized using primers of 27 microsatellite loci to investigate the molecular diversity. The extracted DNA and PCR-based methods were used to amplify the potential microsatellite loci. According to derived results, 61 alleles were amplified including 27 rare and 15 unique alleles and also 88.89% of all studied microsatellite loci were polymorphic. Moreover, di-nucleotide repeat motifs were shown to be more frequent (49.28%) than tri-nucleotide repeat motifs (48.28%). Polymorphism information content (PIC) values were ranged from 0.090 (RM411) to 0.940 (RM105) with an average of 0.531. Furthermore, the UPGMA derived dendrogram grouped all varieties into three major clusters. A high pro...
{"title":"Molecular characterization of Malaysian rice cultivars using SSR markers","authors":"Nurhaziqah Supari, Y. Kaya, Maral Biroudian, M. Javed","doi":"10.1063/1.5125520","DOIUrl":"https://doi.org/10.1063/1.5125520","url":null,"abstract":"Exploring the diversity at the molecular level is one of the prerequisites in modern plant breeding to address the world food security challenges. Malaysian rice cultivars have been characterized using single sequence repeat (SSR) markers to assess the molecular diversity. In this study, 21 Malaysian rice cultivars were characterized using primers of 27 microsatellite loci to investigate the molecular diversity. The extracted DNA and PCR-based methods were used to amplify the potential microsatellite loci. According to derived results, 61 alleles were amplified including 27 rare and 15 unique alleles and also 88.89% of all studied microsatellite loci were polymorphic. Moreover, di-nucleotide repeat motifs were shown to be more frequent (49.28%) than tri-nucleotide repeat motifs (48.28%). Polymorphism information content (PIC) values were ranged from 0.090 (RM411) to 0.940 (RM105) with an average of 0.531. Furthermore, the UPGMA derived dendrogram grouped all varieties into three major clusters. A high pro...","PeriodicalId":20581,"journal":{"name":"PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81680412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations
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