首页 > 最新文献

Renal Failure最新文献

英文 中文
Chrysophanol-mediated trx-1 activation attenuates renal fibrosis through inhibition of the JNK/Cx43 signaling pathway. 菊醇介导的 trx-1 激活可通过抑制 JNK/Cx43 信号通路减轻肾脏纤维化。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1080/0886022X.2024.2398710
Neng Bao, Jin Wang, Qiyu Yue, Fang Cao, Xuejing Gu, Kejian Wen, Wei Kong, Mingjia Gu

Purpose: This study aimed to investigate the inhibitory effect of chrysophanol on renal fibrosis and its molecular mechanism.

Methods: Initially, potential targets of chrysophanol were predicted through network pharmacology analysis, and a protein-protein interaction network of these targets was constructed using Venn diagrams and the STRING database. GO enrichment analysis predicted the biological process of chrysophanol in treating renal fibrosis. Subsequently, both in vivo and in vitro experiments were conducted using unilateral ureteral obstruction (UUO) induced CKD mouse model and HK-2 cell model, respectively. In the mouse model, different doses of chrysophanol were administered to assess its renal protective effects through biochemical indicators, histological examination, and immunofluorescence staining. In the cell model, the regulatory effect of chrysophanol on the Trx-1/JNK/Cx43 pathway was evaluated using western blotting and flow cytometry.

Results: Chrysophanol treatment significantly ameliorated renal dysfunction and histopathological damage in the UUO mouse model, accompanied by a reduction in serum oxidative stress markers. Furthermore, chrysophanol markedly upregulated the expression of Trx-1 in renal tissues and inhibited the activation of the JNK/Cx43 signaling pathway. At the cellular level, chrysophanol enhanced the activity of Trx-1 and downregulated the JNK/Cx43 signaling pathway, thereby inhibiting TGF-β induced oxidative stress and cell apoptosis.

Conclusion: This study demonstrated a significant inhibitory effect of chrysophanol on renal fibrosis, mediated by the activation of Trx-1 to inhibit the JNK/Cx43 pathway. These findings provide experimental support for the potential use of chrysophanol as a therapeutic agent for renal fibrosis.

目的:本研究旨在探讨金丝桃醇对肾脏纤维化的抑制作用及其分子机制:方法:首先通过网络药理学分析预测了金合欢醇的潜在靶点,并利用维恩图和STRING数据库构建了这些靶点的蛋白-蛋白相互作用网络。GO富集分析预测了金丝桃醇治疗肾脏纤维化的生物学过程。随后,分别利用单侧输尿管梗阻(UUO)诱导的CKD小鼠模型和HK-2细胞模型进行了体内和体外实验。在小鼠模型中,给药不同剂量的菊醇,通过生化指标、组织学检查和免疫荧光染色评估其肾脏保护作用。在细胞模型中,利用Western印迹和流式细胞术评估了金丝桃醇对Trx-1/JNK/Cx43通路的调节作用:结果:金丝桃醇能明显改善UUO小鼠模型的肾功能障碍和组织病理学损伤,同时降低血清氧化应激标记物。此外,菊醇还能明显上调肾组织中 Trx-1 的表达,并抑制 JNK/Cx43 信号通路的激活。在细胞水平上,菊醇增强了Trx-1的活性,下调了JNK/Cx43信号通路,从而抑制了TGF-β诱导的氧化应激和细胞凋亡:本研究表明,通过激活 Trx-1,抑制 JNK/Cx43 通路,菊醇对肾脏纤维化有明显的抑制作用。这些发现为可能将金丝桃醇用作肾脏纤维化的治疗药物提供了实验支持。
{"title":"Chrysophanol-mediated trx-1 activation attenuates renal fibrosis through inhibition of the JNK/Cx43 signaling pathway.","authors":"Neng Bao, Jin Wang, Qiyu Yue, Fang Cao, Xuejing Gu, Kejian Wen, Wei Kong, Mingjia Gu","doi":"10.1080/0886022X.2024.2398710","DOIUrl":"10.1080/0886022X.2024.2398710","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the inhibitory effect of chrysophanol on renal fibrosis and its molecular mechanism.</p><p><strong>Methods: </strong>Initially, potential targets of chrysophanol were predicted through network pharmacology analysis, and a protein-protein interaction network of these targets was constructed using Venn diagrams and the STRING database. GO enrichment analysis predicted the biological process of chrysophanol in treating renal fibrosis. Subsequently, both <i>in vivo</i> and <i>in vitro</i> experiments were conducted using unilateral ureteral obstruction (UUO) induced CKD mouse model and HK-2 cell model, respectively. In the mouse model, different doses of chrysophanol were administered to assess its renal protective effects through biochemical indicators, histological examination, and immunofluorescence staining. In the cell model, the regulatory effect of chrysophanol on the Trx-1/JNK/Cx43 pathway was evaluated using western blotting and flow cytometry.</p><p><strong>Results: </strong>Chrysophanol treatment significantly ameliorated renal dysfunction and histopathological damage in the UUO mouse model, accompanied by a reduction in serum oxidative stress markers. Furthermore, chrysophanol markedly upregulated the expression of Trx-1 in renal tissues and inhibited the activation of the JNK/Cx43 signaling pathway. At the cellular level, chrysophanol enhanced the activity of Trx-1 and downregulated the JNK/Cx43 signaling pathway, thereby inhibiting TGF-β induced oxidative stress and cell apoptosis.</p><p><strong>Conclusion: </strong>This study demonstrated a significant inhibitory effect of chrysophanol on renal fibrosis, mediated by the activation of Trx-1 to inhibit the JNK/Cx43 pathway. These findings provide experimental support for the potential use of chrysophanol as a therapeutic agent for renal fibrosis.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11382722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Right ventricular-pulmonary artery uncoupling in patients with atrial fibrillation on peritoneal dialysis. 腹膜透析心房颤动患者的右心室-肺动脉解偶联。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-11 DOI: 10.1080/0886022X.2024.2413872
Tao Zhang, Zijun Zhou, Qianyi Zhou, Jie Li, Zhiwei Zhang, Shili Cao, Bo Yang, Qingmiao Shao

Background: Tricuspid annular plane systolic excursion (TAPSE)/pulmonary artery systolic pressure (PASP) as a noninvasively measured index of right ventricular-pulmonary artery uncoupling is associated with poor outcomes in heart failure patients. However, the relationship by which the TAPSE/PASP is linked to atrial fibrillation (AF) in peritoneal dialysis (PD) patients is not clear. We aimed to investigate the relationship between the TAPSE/PASP and AF in PD patients.

Methods: This study was divided into two parts. First, we included 329 PD patients. All the subjects provided detailed a medical history, laboratory analysis and transthoracic echocardiography on admission. We evaluated the differences in the TASPE/PASP ratios between the AF and non-AF groups. Second, a total of 121 patients were followed up to compare mortality between the AF and non-AF groups.

Results: Age, BNP, RDW, LA, and septal E/e' were significantly higher, and TAPSE/PASP was significantly lower in patients with AF than in those without AF (p < 0.05). Moreover, the TAPSE/PASP was more pronounced in persistent AF patients. PD patients with AF had a greater risk of mortality (7.2%) than did those without AF (3.8%) after an average follow-up of 12 months. Kaplan-Meier analysis revealed that patients with TAPSE/PASP ratios ≤ 0.715 had a greater risk of mortality than did those with TAPSE/PASP ratios > 0.715.

Conclusions: The results suggested that the TAPSE/PASP was lower in AF patients than in non-AF patients. The TAPSE/PASP may be a useful factor for predicting mortality in AF patients with PD, but large-scale prospective studies are needed for verification.

背景:三尖瓣环平面收缩期偏移(TAPSE)/肺动脉收缩压(PASP)作为右心室-肺动脉解偶联的无创性测量指标,与心衰患者的不良预后有关。然而,TAPSE/PASP 与腹膜透析(PD)患者心房颤动(AF)的关系尚不清楚。我们旨在研究腹膜透析患者的 TAPSE/PASP 与房颤之间的关系:本研究分为两部分。首先,我们纳入了 329 名腹膜透析患者。所有受试者在入院时均提供了详细的病史、实验室分析和经胸超声心动图。我们评估了房颤组和非房颤组之间 TASPE/PASP 比率的差异。其次,我们对 121 名患者进行了随访,以比较心房颤动组和非心房颤动组的死亡率:结果:房颤患者的年龄、BNP、RDW、LA和室间隔E/e'显著高于非房颤患者,TAPSE/PASP显著低于非房颤患者(P 0.715):结果表明,房颤患者的 TAPSE/PASP 低于非房颤患者。TAPSE/PASP可能是预测患有腹膜透析的房颤患者死亡率的有用因素,但还需要大规模的前瞻性研究来验证。
{"title":"Right ventricular-pulmonary artery uncoupling in patients with atrial fibrillation on peritoneal dialysis.","authors":"Tao Zhang, Zijun Zhou, Qianyi Zhou, Jie Li, Zhiwei Zhang, Shili Cao, Bo Yang, Qingmiao Shao","doi":"10.1080/0886022X.2024.2413872","DOIUrl":"10.1080/0886022X.2024.2413872","url":null,"abstract":"<p><strong>Background: </strong>Tricuspid annular plane systolic excursion (TAPSE)/pulmonary artery systolic pressure (PASP) as a noninvasively measured index of right ventricular-pulmonary artery uncoupling is associated with poor outcomes in heart failure patients. However, the relationship by which the TAPSE/PASP is linked to atrial fibrillation (AF) in peritoneal dialysis (PD) patients is not clear. We aimed to investigate the relationship between the TAPSE/PASP and AF in PD patients.</p><p><strong>Methods: </strong>This study was divided into two parts. First, we included 329 PD patients. All the subjects provided detailed a medical history, laboratory analysis and transthoracic echocardiography on admission. We evaluated the differences in the TASPE/PASP ratios between the AF and non-AF groups. Second, a total of 121 patients were followed up to compare mortality between the AF and non-AF groups.</p><p><strong>Results: </strong>Age, BNP, RDW, LA, and septal E/e' were significantly higher, and TAPSE/PASP was significantly lower in patients with AF than in those without AF (<i>p</i> < 0.05). Moreover, the TAPSE/PASP was more pronounced in persistent AF patients. PD patients with AF had a greater risk of mortality (7.2%) than did those without AF (3.8%) after an average follow-up of 12 months. Kaplan-Meier analysis revealed that patients with TAPSE/PASP ratios ≤ 0.715 had a greater risk of mortality than did those with TAPSE/PASP ratios > 0.715.</p><p><strong>Conclusions: </strong>The results suggested that the TAPSE/PASP was lower in AF patients than in non-AF patients. The TAPSE/PASP may be a useful factor for predicting mortality in AF patients with PD, but large-scale prospective studies are needed for verification.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of a novel nonsense mutation in α-galactosidase A that causes Fabry disease in a Chinese family. 在一个中国家庭中鉴定出一种导致法布里病的α-半乳糖苷酶A新型无义突变。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-06-07 DOI: 10.1080/0886022X.2024.2362391
Yushi Peng, Meize Pan, Yuchen Wang, Zongrui Shen, Jian Xu, Fu Xiong, Hongbo Xiao, Yun Miao

Fabry disease, a lysosomal storage disease, is an uncommon X-linked recessive genetic disorder stemming from abnormalities in the alpha-galactosidase gene (GLA) that codes human alpha-Galactosidase A (α-Gal A). To date, over 800 GLA mutations have been found to cause Fabry disease (FD). Continued enhancement of the GLA mutation spectrum will contribute to a deeper recognition and underlying mechanisms of FD. In this study, a 27-year-old male proband exhibited a typical phenotype of Fabry disease. Subsequently, family screening for Fabry disease was conducted, and high-throughput sequencing was employed to identify the mutated gene. The three-level structure of the mutated protein was analyzed, and its subcellular localization and enzymatic activity were determined. Apoptosis was assessed in GLA mutant cell lines to confirm the functional effects. As a result, a new mutation, c.777_778del (p. Gly261Leufs*3), in the GLA gene was identified. The mutation caused a frameshift during translation and the premature appearance of a termination codon, which led to a partial deletion of the domain in C-terminal region and altered the protein's tertiary structure. In vitro experiments revealed a significant reduction of the enzymatic activity in mutant cells. The expression was noticeably decreased at the mRNA and protein levels in mutant cell lines. Additionally, the subcellular localization of α-Gal A changed from a homogeneous distribution to punctate aggregation in the cytoplasm. GLA mutant cells exhibited significantly higher levels of apoptosis compared to wild-type cells.

法布里病是一种溶酶体贮积病,是一种不常见的 X 连锁隐性遗传疾病,源于编码人类α-半乳糖苷酶 A(α-Gal A)的α-半乳糖苷酶基因(GLA)异常。迄今为止,已发现 800 多种 GLA 基因突变可导致法布里病(FD)。不断扩大 GLA 基因突变谱将有助于更深入地认识法布里病及其潜在机制。在本研究中,一名 27 岁的男性原患者表现出典型的法布里病表型。随后,进行了法布里病家族筛查,并采用高通量测序鉴定了突变基因。对突变蛋白的三级结构进行了分析,并确定了其亚细胞定位和酶活性。评估了GLA突变细胞系的凋亡情况,以确认其功能效应。结果,在 GLA 基因中发现了一个新的突变,即 c.777_778del (p. Gly261Leufs*3)。该突变导致翻译过程中的框架偏移和终止密码子的过早出现,从而导致 C 端区域结构域的部分缺失,并改变了蛋白质的三级结构。体外实验显示,突变体细胞的酶活性显著降低。突变体细胞系的 mRNA 和蛋白质水平的表达量明显下降。此外,α-Gal A 的亚细胞定位从均匀分布变为细胞质中的点状聚集。与野生型细胞相比,GLA突变型细胞的凋亡水平明显更高。
{"title":"Identification of a novel nonsense mutation in α-galactosidase A that causes Fabry disease in a Chinese family.","authors":"Yushi Peng, Meize Pan, Yuchen Wang, Zongrui Shen, Jian Xu, Fu Xiong, Hongbo Xiao, Yun Miao","doi":"10.1080/0886022X.2024.2362391","DOIUrl":"10.1080/0886022X.2024.2362391","url":null,"abstract":"<p><p>Fabry disease, a lysosomal storage disease, is an uncommon X-linked recessive genetic disorder stemming from abnormalities in the alpha-galactosidase gene (<i>GLA</i>) that codes human alpha-Galactosidase A (α-Gal A). To date, over 800 <i>GLA</i> mutations have been found to cause Fabry disease (FD). Continued enhancement of the <i>GLA</i> mutation spectrum will contribute to a deeper recognition and underlying mechanisms of FD. In this study, a 27-year-old male proband exhibited a typical phenotype of Fabry disease. Subsequently, family screening for Fabry disease was conducted, and high-throughput sequencing was employed to identify the mutated gene. The three-level structure of the mutated protein was analyzed, and its subcellular localization and enzymatic activity were determined. Apoptosis was assessed in <i>GLA</i> mutant cell lines to confirm the functional effects. As a result, a new mutation, c.777_778del (p. Gly261Leufs*3), in the <i>GLA</i> gene was identified. The mutation caused a frameshift during translation and the premature appearance of a termination codon, which led to a partial deletion of the domain in C-terminal region and altered the protein's tertiary structure. <i>In vitro</i> experiments revealed a significant reduction of the enzymatic activity in mutant cells. The expression was noticeably decreased at the mRNA and protein levels in mutant cell lines. Additionally, the subcellular localization of α-Gal A changed from a homogeneous distribution to punctate aggregation in the cytoplasm. <i>GLA</i> mutant cells exhibited significantly higher levels of apoptosis compared to wild-type cells.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Factors associated with dysfunction of autogenous arteriovenous fistula in patients with secondary hyperparathyroidism after parathyroidectomy. 甲状旁腺切除术后继发性甲状旁腺功能亢进症患者自体动静脉瘘功能障碍的相关因素
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1080/0886022X.2024.2402515
Boxi Chen, Qiying Fang, Yiming Tao, Siqi Peng, Shuting Deng, Ye Yuan, Nan Jiang, Sichun Wen, Bohou Li, Qiong Wu, Zewen Zhao, Pingjiang Ge, Sijia Li, Ting Lin, Zhonglin Feng, Feng Wen, Lei Fu, Zhuo Li, Jia Wen, Renwei Huang, Chaosheng He, Wenjian Wang, Guibao Ke, Lixia Xu, Shuangxin Liu, Jianchao Ma

Background: Secondary hyperparathyroidism (SHPT) is a prevalent chronic complication in patients undergoing hemodialysis. Parathyroidectomy (PTX) is crucial for reducing mortality and improving the prognosis in the treatment of refractory hyperparathyroidism. However, it is often associated with a number of postoperative complications such as postoperative hypotension, hyperkalemia, and hungry bone syndrome. A previous study demonstrated that low blood pressure influences the patency of autogenous arteriovenous fistulas (AVF). Few studies have examined AVF dysfunction following PTX. This study aimed to identify and describe the risk variables associated with AVF dysfunction after PTX.

Methods: Cases of AVF dysfunction after PTX between 2015 and 2021 were studied. Four controls were identified for each patient and were matched for sex and age. Biochemical parameters and blood pressure of the patients before and after PTX were recorded. Risk factors for AVF dysfunction after PTX were identified using conditional logistic regression analysis.

Results: Sixteen patients and 64 controls were included in this study. Baseline demographic and laboratory data were compared. Patients in the AVF dysfunction group had lower levels of postoperative calcium than the controls. After surgery, calcium levels decreased more in patients with AVF dysfunction than in the control group. The decrease in systolic blood pressure (ΔSBP) after PTX was greater in the AVF dysfunction group than that in the control group. For each 1 mmHg increment in ΔSBP, the risk of AVF dysfunction after surgery increased by 11.6% (OR = 1.116, 95% CI, 1.005-1.239, p = .040). The likelihood of developing AVF dysfunction after surgery was twelvefold higher in diabetic patients than in non-diabetic patients (OR = 12.506, 95% CI, 1.113-140.492, p = .041). Among patients with ΔSBP > 5.8 mmHg after PTX, the AVF failure rate was significantly greater in patients with diabetes than in those without diabetes. Patients with a history of AVF failure had a nine-fold higher risk of developing AVF dysfunction (OR = 9.143, 95% CI, 1.151-72.627, p = .036). Serum albumin, hemoglobin, ΔiPTH, and age were not independent predictors of AVF dysfunction. The cutoff value for SBP was 5.8 mmHg, as determined by the Youden index of the receiver operating characteristic curve.

Conclusion: Decreased systolic blood pressure (ΔSBP) after PTX, diabetes, and AVF failure history were risk factors for AVF dysfunction following PTX in patients with SHPT. Diabetes patients with ΔSBP > 5.8 mmHg were more prone to AVF dysfunction after PTX.

背景:继发性甲状旁腺功能亢进症(SHPT继发性甲状旁腺功能亢进症(SHPT)是血液透析患者普遍存在的一种慢性并发症。在治疗难治性甲状旁腺功能亢进症时,甲状旁腺切除术(PTX)对于降低死亡率和改善预后至关重要。然而,PTX术后往往会出现一系列并发症,如术后低血压、高钾血症和饿骨综合征。之前的一项研究表明,低血压会影响自体动静脉瘘(AVF)的通畅性。很少有研究对 PTX 术后动静脉瘘功能障碍进行研究。本研究旨在确定和描述与 PTX 后动静脉瘘功能障碍相关的风险变量:方法:研究了 2015 年至 2021 年间 PTX 术后出现动静脉瘘功能障碍的病例。每名患者确定四名对照组,并进行性别和年龄匹配。记录PTX前后患者的生化指标和血压。通过条件逻辑回归分析确定了 PTX 后动静脉瘘功能障碍的风险因素:本研究共纳入 16 名患者和 64 名对照组。比较了基线人口统计学和实验室数据。动静脉瘘功能障碍组患者术后血钙水平低于对照组。术后,动静脉瓣膜功能障碍患者的血钙水平下降幅度大于对照组。PTX 术后,动静脉瓣膜功能障碍组收缩压(ΔSBP)的下降幅度大于对照组。ΔSBP 每增加 1 mmHg,术后发生动静脉瓣膜功能障碍的风险增加 11.6%(OR = 1.116,95% CI,1.005-1.239,p = .040)。糖尿病患者术后发生动静脉瓣膜功能障碍的可能性是非糖尿病患者的 12 倍(OR = 12.506,95% CI,1.113-140.492,p = .041)。在 PTX 后ΔSBP > 5.8 mmHg 的患者中,糖尿病患者的动静脉瓣膜失败率明显高于非糖尿病患者。有动静脉瘘失败史的患者发生动静脉瘘功能障碍的风险高出九倍(OR = 9.143,95% CI,1.151-72.627,p = .036)。血清白蛋白、血红蛋白、ΔiPTH 和年龄不是 AVF 功能障碍的独立预测因素。根据接收者操作特征曲线的尤登指数,SBP 的临界值为 5.8 mmHg:结论:PTX 后收缩压下降(ΔSBP)、糖尿病和 AVF 衰竭史是 SHPT 患者 PTX 后 AVF 功能障碍的危险因素。ΔSBP>5.8 mmHg的糖尿病患者在 PTX 后更容易出现动静脉瓣膜功能障碍。
{"title":"Factors associated with dysfunction of autogenous arteriovenous fistula in patients with secondary hyperparathyroidism after parathyroidectomy.","authors":"Boxi Chen, Qiying Fang, Yiming Tao, Siqi Peng, Shuting Deng, Ye Yuan, Nan Jiang, Sichun Wen, Bohou Li, Qiong Wu, Zewen Zhao, Pingjiang Ge, Sijia Li, Ting Lin, Zhonglin Feng, Feng Wen, Lei Fu, Zhuo Li, Jia Wen, Renwei Huang, Chaosheng He, Wenjian Wang, Guibao Ke, Lixia Xu, Shuangxin Liu, Jianchao Ma","doi":"10.1080/0886022X.2024.2402515","DOIUrl":"https://doi.org/10.1080/0886022X.2024.2402515","url":null,"abstract":"<p><strong>Background: </strong>Secondary hyperparathyroidism (SHPT) is a prevalent chronic complication in patients undergoing hemodialysis. Parathyroidectomy (PTX) is crucial for reducing mortality and improving the prognosis in the treatment of refractory hyperparathyroidism. However, it is often associated with a number of postoperative complications such as postoperative hypotension, hyperkalemia, and hungry bone syndrome. A previous study demonstrated that low blood pressure influences the patency of autogenous arteriovenous fistulas (AVF). Few studies have examined AVF dysfunction following PTX. This study aimed to identify and describe the risk variables associated with AVF dysfunction after PTX.</p><p><strong>Methods: </strong>Cases of AVF dysfunction after PTX between 2015 and 2021 were studied. Four controls were identified for each patient and were matched for sex and age. Biochemical parameters and blood pressure of the patients before and after PTX were recorded. Risk factors for AVF dysfunction after PTX were identified using conditional logistic regression analysis.</p><p><strong>Results: </strong>Sixteen patients and 64 controls were included in this study. Baseline demographic and laboratory data were compared. Patients in the AVF dysfunction group had lower levels of postoperative calcium than the controls. After surgery, calcium levels decreased more in patients with AVF dysfunction than in the control group. The decrease in systolic blood pressure (ΔSBP) after PTX was greater in the AVF dysfunction group than that in the control group. For each 1 mmHg increment in ΔSBP, the risk of AVF dysfunction after surgery increased by 11.6% (OR = 1.116, 95% CI, 1.005-1.239, <i>p</i> = .040). The likelihood of developing AVF dysfunction after surgery was twelvefold higher in diabetic patients than in non-diabetic patients (OR = 12.506, 95% CI, 1.113-140.492, <i>p</i> = .041). Among patients with ΔSBP > 5.8 mmHg after PTX, the AVF failure rate was significantly greater in patients with diabetes than in those without diabetes. Patients with a history of AVF failure had a nine-fold higher risk of developing AVF dysfunction (OR = 9.143, 95% CI, 1.151-72.627, <i>p</i> = .036). Serum albumin, hemoglobin, ΔiPTH, and age were not independent predictors of AVF dysfunction. The cutoff value for SBP was 5.8 mmHg, as determined by the Youden index of the receiver operating characteristic curve.</p><p><strong>Conclusion: </strong>Decreased systolic blood pressure (ΔSBP) after PTX, diabetes, and AVF failure history were risk factors for AVF dysfunction following PTX in patients with SHPT. Diabetes patients with ΔSBP > 5.8 mmHg were more prone to AVF dysfunction after PTX.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cumulative rabbit anti-human thymocyte globulin dose to recipient weight during the peri-operative period is an independent risk factor for early postoperative urinary tract infection after kidney transplantation. 肾移植术后早期尿路感染的独立风险因素是围手术期累积的兔抗人胸腺细胞球蛋白剂量与受体体重之比。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1080/0886022X.2024.2414841
Shujuan Li, Ziyu Wang, Zhen Dong, Yanwei Cao, Hongyang Wang

Anti-human thymocyte globulin-Fresenius (ATG-F) is frequently utilized to achieve successful induction for kidney transplantation recipients. This study aimed to examine the association between the ATG-F dose-to-recipient-weight ratio (ADR) and the risk of developing urinary tract infections (UTIs) following kidney transplantation. Data of kidney transplant recipients who underwent ATG-F-induction peri-operatively in a medical center were retrospectively collected, and the incidence of UTIs during the first postoperative year was also recorded. The risk of UTI associated with ADR was analyzed, and receiver operating characteristic curves were drawn to determine the optimal ADR, followed by Cox regression models. In total, 131 recipients were included, with an UTI incidence of 19.08% and a mean interval of 3.08 months. The optimal ADR was 6.34, involving 41 and 90 patients in the low ADR and high ADR groups, respectively. The UTI-free rate in the low ADR group was significantly higher than that in the high ADR group (p = 0.007). Cox regression analysis indicated that a high ADR independently increased the risk of UTI following kidney transplantation (hazard ratio: 5.306, 95% confidence interval: 1.243-22.660, p = 0.024). There was no significant difference in rejection rate between the high ADR and low ADR groups. In conclusion, a high ADR increased the incidence of early postoperative UTI among kidney transplant recipients.

抗人胸腺细胞球蛋白-费森尤斯(ATG-F)经常用于肾移植受者的成功诱导。本研究旨在探讨ATG-F剂量与受者体重比(ADR)与肾移植术后发生尿路感染(UTI)风险之间的关系。研究人员回顾性收集了一家医疗中心围手术期接受ATG-F诱导的肾移植受者的数据,并记录了术后第一年的UTI发病率。分析了与 ADR 相关的 UTI 风险,并绘制了接收者操作特征曲线以确定最佳 ADR,随后建立了 Cox 回归模型。共纳入了 131 名受术者,UTI 发生率为 19.08%,平均间隔时间为 3.08 个月。最佳 ADR 为 6.34,低 ADR 组和高 ADR 组分别有 41 和 90 名患者。低 ADR 组的无尿毒症率明显高于高 ADR 组(P = 0.007)。Cox 回归分析表明,高 ADR 会独立增加肾移植后发生 UTI 的风险(危险比:5.306,95% 置信区间:1.243-22.660,p = 0.024)。高 ADR 组和低 ADR 组的排斥率没有明显差异。总之,高 ADR 会增加肾移植受者术后早期尿毒症的发病率。
{"title":"Cumulative rabbit anti-human thymocyte globulin dose to recipient weight during the peri-operative period is an independent risk factor for early postoperative urinary tract infection after kidney transplantation.","authors":"Shujuan Li, Ziyu Wang, Zhen Dong, Yanwei Cao, Hongyang Wang","doi":"10.1080/0886022X.2024.2414841","DOIUrl":"https://doi.org/10.1080/0886022X.2024.2414841","url":null,"abstract":"<p><p>Anti-human thymocyte globulin-Fresenius (ATG-F) is frequently utilized to achieve successful induction for kidney transplantation recipients. This study aimed to examine the association between the ATG-F dose-to-recipient-weight ratio (ADR) and the risk of developing urinary tract infections (UTIs) following kidney transplantation. Data of kidney transplant recipients who underwent ATG-F-induction peri-operatively in a medical center were retrospectively collected, and the incidence of UTIs during the first postoperative year was also recorded. The risk of UTI associated with ADR was analyzed, and receiver operating characteristic curves were drawn to determine the optimal ADR, followed by Cox regression models. In total, 131 recipients were included, with an UTI incidence of 19.08% and a mean interval of 3.08 months. The optimal ADR was 6.34, involving 41 and 90 patients in the low ADR and high ADR groups, respectively. The UTI-free rate in the low ADR group was significantly higher than that in the high ADR group (<i>p</i> = 0.007). Cox regression analysis indicated that a high ADR independently increased the risk of UTI following kidney transplantation (hazard ratio: 5.306, 95% confidence interval: 1.243-22.660, <i>p</i> = 0.024). There was no significant difference in rejection rate between the high ADR and low ADR groups. In conclusion, a high ADR increased the incidence of early postoperative UTI among kidney transplant recipients.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The role of moderate to vigorous physical activity level and number of treatments/medications in mediating the effect of body mass index on diabetic nephropathy: a Mendelian randomization study. 中度至剧烈运动水平和治疗/药物数量在调解体重指数对糖尿病肾病影响中的作用:孟德尔随机研究。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI: 10.1080/0886022X.2024.2417738
Shasha Hu, Yuling Chen, Mingjie He, Jun Wen, Aimin Zhong, Dandan Zhan, Zhibin Ye

Background: Body mass index (BMI) is associated with diabetic nephropathy (DN). However, the mediator factors in the BMI-DN effects remain unclear.

Methods: Univariate and multivariate Mendelian randomization (MR) analysis were performed to estimate the association between six lifestyles (moderate to vigorous physical activity levels, years of schooling, BMI, nap during day, number of treatments/medications taken and coffee intake) and DN. MR Egger, Weighted median, Simple mode, and Weighted mode was supplemental methods to Inverse variance weighted. Sensitivity analysis included heterogeneity test, horizontal pleiotropy test, and Leave-One-Out. Additionally, mediation MR was conducted to evaluate the mediating role of lifestyles between BMI and DN. Finally, functional enrichment analysis based on the mediation MR results was performed.

Results: univariate and multivariate Mendelian randomization (MR) analysis were performed to estimate the association between six lifestyles (moderate to vigorous physical activity levels, years of schooling, BMI, nap during day, number of treatments/medications taken and coffee intake) and DN. MR Egger, Weighted median, Simple mode, and Weighted mode was supplemental methods to Inverse variance weighted. Sensitivity analysis included heterogeneity test, horizontal pleiotropy test, and Leave-One-Out. Additionally, mediation MR was conducted to evaluate the mediating role of lifestyles between BMI and DN. Finally, functional enrichment analysis based on the mediation MR results was performed.

Conclusion: our results supported mediation role of vigorous physical activity level and number of treatments/medications in BMI-DN effects.

背景:体重指数(BMI)与糖尿病肾病(DN)有关。然而,BMI-DN效应的中介因素仍不清楚:方法:进行单变量和多变量孟德尔随机化(MR)分析,以估计六种生活方式(中度至剧烈运动水平、受教育年限、体重指数、白天午睡、接受治疗/药物的次数和咖啡摄入量)与 DN 之间的关系。MR Egger、加权中位数、简单模式和加权模式是逆方差加权的补充方法。敏感性分析包括异质性检验、水平多向性检验和 "一出一进 "检验。此外,还进行了中介 MR 分析,以评估生活方式在 BMI 和 DN 之间的中介作用。结果:进行了单变量和多变量孟德尔随机化(MR)分析,以估计六种生活方式(中强度体力活动水平、受教育年限、体重指数、白天午睡、接受治疗/药物的次数和咖啡摄入量)与 DN 之间的关系。MR Egger、加权中位数、简单模式和加权模式是逆方差加权的补充方法。敏感性分析包括异质性检验、水平多向性检验和一出一进检验。此外,还进行了中介 MR 分析,以评估生活方式在 BMI 和 DN 之间的中介作用。结论:我们的研究结果支持剧烈运动水平和治疗/药物次数在 BMI-DN 影响中的中介作用。
{"title":"The role of moderate to vigorous physical activity level and number of treatments/medications in mediating the effect of body mass index on diabetic nephropathy: a Mendelian randomization study.","authors":"Shasha Hu, Yuling Chen, Mingjie He, Jun Wen, Aimin Zhong, Dandan Zhan, Zhibin Ye","doi":"10.1080/0886022X.2024.2417738","DOIUrl":"10.1080/0886022X.2024.2417738","url":null,"abstract":"<p><strong>Background: </strong>Body mass index (BMI) is associated with diabetic nephropathy (DN). However, the mediator factors in the BMI-DN effects remain unclear.</p><p><strong>Methods: </strong>Univariate and multivariate Mendelian randomization (MR) analysis were performed to estimate the association between six lifestyles (moderate to vigorous physical activity levels, years of schooling, BMI, nap during day, number of treatments/medications taken and coffee intake) and DN. MR Egger, Weighted median, Simple mode, and Weighted mode was supplemental methods to Inverse variance weighted. Sensitivity analysis included heterogeneity test, horizontal pleiotropy test, and Leave-One-Out. Additionally, mediation MR was conducted to evaluate the mediating role of lifestyles between BMI and DN. Finally, functional enrichment analysis based on the mediation MR results was performed.</p><p><strong>Results: </strong>univariate and multivariate Mendelian randomization (MR) analysis were performed to estimate the association between six lifestyles (moderate to vigorous physical activity levels, years of schooling, BMI, nap during day, number of treatments/medications taken and coffee intake) and DN. MR Egger, Weighted median, Simple mode, and Weighted mode was supplemental methods to Inverse variance weighted. Sensitivity analysis included heterogeneity test, horizontal pleiotropy test, and Leave-One-Out. Additionally, mediation MR was conducted to evaluate the mediating role of lifestyles between BMI and DN. Finally, functional enrichment analysis based on the mediation MR results was performed.</p><p><strong>Conclusion: </strong>our results supported mediation role of vigorous physical activity level and number of treatments/medications in BMI-DN effects.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and validation of a chronic kidney disease progression model using patient-level simulations. 利用患者层面的模拟,开发并验证慢性肾病进展模型。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-21 DOI: 10.1080/0886022X.2024.2406402
Mafalda Ramos, Laetitia Gerlier, Anastasia Uster, Louise Muttram, Dominik Steubl, Andrew H Frankel, Mark Lamotte

Chronic disease progression models are available for several highly prevalent conditions. For chronic kidney disease (CKD), the scope of existing progression models is limited to the risk of kidney failure and major cardiovascular (CV) events. The aim of this project was to develop a comprehensive CKD progression model (CKD-PM) that simulates the risk of CKD progression and a broad range of complications in patients with CKD. A series of literature reviews informed the selection of risk factors and identified existing risk equations/algorithms for kidney replacement therapy (KRT), CV events, other CKD-related complications, and mortality. Risk equations and transition probabilities were primarily sourced from publications produced by large US and international CKD registries. A patient-level, state-transition model was developed with health states defined by the Kidney Disease Improving Global Outcomes categories. Model validation was performed by comparing predicted outcomes with observed outcomes in the source cohorts used in model development (internal validation) and other cohorts (external validation). The CKD-PM demonstrated satisfactory modeling properties. Accurate prediction of all-cause and CV mortality was achieved without calibration, while prediction of CV events through CKD-specific equations required implementation of a calibration factor to balance time-dependent versus baseline risk. Predicted annual changes in estimated glomerular filtration rate (eGFR) and urine albumin-creatinine ratio were acceptable in comparison to external values. A flexible eGFR threshold for KRT equations enabled accurate prediction of these events. This CKD-PM demonstrated reliable modeling properties. Both internal and external validation revealed robust outcomes.

慢性疾病进展模型适用于几种高发疾病。对于慢性肾脏病(CKD),现有进展模型的范围仅限于肾衰竭和主要心血管(CV)事件的风险。本项目旨在开发一种全面的慢性肾脏病进展模型(CKD-PM),以模拟慢性肾脏病进展风险和慢性肾脏病患者的各种并发症。在选择风险因素时参考了一系列文献综述,并确定了肾脏替代疗法 (KRT)、冠心病事件、其他 CKD 相关并发症和死亡率的现有风险方程/算法。风险方程和转换概率主要来源于美国和国际大型 CKD 登记处的出版物。根据 "肾脏病改善全球结果 "类别所定义的健康状态,建立了患者级别的状态转换模型。通过比较模型开发中使用的源队列(内部验证)和其他队列(外部验证)的预测结果与观察结果,对模型进行了验证。CKD-PM 的建模特性令人满意。无需校准就能准确预测全因死亡率和冠心病死亡率,而通过 CKD 特定方程预测冠心病事件则需要使用校准因子来平衡时间依赖性风险和基线风险。与外部值相比,预测的估计肾小球滤过率(eGFR)和尿白蛋白-肌酐比值的年度变化是可以接受的。用于 KRT 方程的灵活的 eGFR 阈值能够准确预测这些事件。该 CKD-PM 具有可靠的建模特性。内部和外部验证均显示了可靠的结果。
{"title":"Development and validation of a chronic kidney disease progression model using patient-level simulations.","authors":"Mafalda Ramos, Laetitia Gerlier, Anastasia Uster, Louise Muttram, Dominik Steubl, Andrew H Frankel, Mark Lamotte","doi":"10.1080/0886022X.2024.2406402","DOIUrl":"10.1080/0886022X.2024.2406402","url":null,"abstract":"<p><p>Chronic disease progression models are available for several highly prevalent conditions. For chronic kidney disease (CKD), the scope of existing progression models is limited to the risk of kidney failure and major cardiovascular (CV) events. The aim of this project was to develop a comprehensive CKD progression model (CKD-PM) that simulates the risk of CKD progression and a broad range of complications in patients with CKD. A series of literature reviews informed the selection of risk factors and identified existing risk equations/algorithms for kidney replacement therapy (KRT), CV events, other CKD-related complications, and mortality. Risk equations and transition probabilities were primarily sourced from publications produced by large US and international CKD registries. A patient-level, state-transition model was developed with health states defined by the Kidney Disease Improving Global Outcomes categories. Model validation was performed by comparing predicted outcomes with observed outcomes in the source cohorts used in model development (internal validation) and other cohorts (external validation). The CKD-PM demonstrated satisfactory modeling properties. Accurate prediction of all-cause and CV mortality was achieved without calibration, while prediction of CV events through CKD-specific equations required implementation of a calibration factor to balance time-dependent versus baseline risk. Predicted annual changes in estimated glomerular filtration rate (eGFR) and urine albumin-creatinine ratio were acceptable in comparison to external values. A flexible eGFR threshold for KRT equations enabled accurate prediction of these events. This CKD-PM demonstrated reliable modeling properties. Both internal and external validation revealed robust outcomes.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11494709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HDAC6 promotes inflammation in lupus nephritis mice by regulating transcription factors MAFF and KLF5 in renal fibrosis. HDAC6 通过调节肾脏纤维化中的转录因子 MAFF 和 KLF5 促进狼疮肾炎小鼠的炎症。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1080/0886022X.2024.2415517
Meihui Deng, Xiao Tan, Xiaojie Peng, Weimin Zheng, Rui Fu, Shanshan Tao

Aim: This study explored the effect and mechanism of MAFF and HDAC6 on renal fibrosis and inflammation in lupus nephritis (LN).

Methods: IL-33 treated renal epithelial cells and MRL/lpr mice were respectively used for in vitro and in vivo experiments. The expressions of HDAC6, MAFF, and KLF5 were measured in cells and renal tissues. Before and after cell transfection, the morphological changes in renal tissues were observed using Hematoxylin and eosin (H&E) and Masson staining. The proteinuria, serum creatinine (SCr), blood urea nitrogen (BUN), and double-stranded DNA (dsDNA) levels were detected by biochemical analysis. The expressions of fibrosis and inflammation related proteins (including α-SMA, Vimentin, IL-1β, IL-6, and TNF-α), p65, and iNOS were also detected. The relationship among MAFF, HDAC6, and KLF5 was determined by chromatin immunoprecipitation and dual luciferase reporter gene assay.

Results: Renal tissues and cell models had elevated expressions of HDAC6 and KLF5, and decreased MAFF expression. HDAC6 suppression or MAFF overexpression led to suppression of proteinuria, SCr, BUN, and dsDNA levels, as well as attenuation of inflammatory infiltration and collagen deposition. HDAC6 can suppress MAFF expression via deacetylation to abolish its suppression of KLF5 expression, thus increasing KLF5 expression. In vivo and in vitro experiments showed the suppressive effect of HDAC6 suppression on renal fibrosis and inflammation can be abolished by KLF5 overexpression.

Conclusion: HDAC6 suppresses MAFF expression via deacetylation to elevate KLF5 expression, which consequently enhances fibrosis and inflammatory response in LN.

目的:本研究探讨了MAFF和HDAC6对狼疮性肾炎(LN)肾脏纤维化和炎症的影响及机制:方法:分别使用经 IL-33 处理的肾上皮细胞和 MRL/lpr 小鼠进行体外和体内实验。方法:分别用 IL-33 处理过的肾上皮细胞和 MRL/lpr 小鼠进行体外和体内实验,测定细胞和肾组织中 HDAC6、MAFF 和 KLF5 的表达。在细胞转染前后,使用苏木精(Hematoxylin and eosin,H&E)和马森(Masson)染色法观察肾组织的形态学变化。生化分析检测了蛋白尿、血清肌酐(SCr)、血尿素氮(BUN)和双链DNA(dsDNA)水平。此外,还检测了纤维化和炎症相关蛋白(包括α-SMA、Vimentin、IL-1β、IL-6 和 TNF-α)、p65 和 iNOS 的表达。通过染色质免疫沉淀和双荧光素酶报告基因检测确定了MAFF、HDAC6和KLF5之间的关系:结果:肾组织和细胞模型的 HDAC6 和 KLF5 表达升高,MAFF 表达降低。抑制 HDAC6 或 MAFF 过度表达可抑制蛋白尿、SCr、BUN 和 dsDNA 水平,减轻炎症浸润和胶原沉积。HDAC6 可通过去乙酰化抑制 MAFF 的表达,从而取消其对 KLF5 表达的抑制作用,增加 KLF5 的表达。体内和体外实验表明,HDAC6对肾脏纤维化和炎症的抑制作用可被KLF5过表达所取消:结论:HDAC6通过去乙酰化抑制MAFF的表达,从而提高KLF5的表达,进而增强LN的纤维化和炎症反应。
{"title":"HDAC6 promotes inflammation in lupus nephritis mice by regulating transcription factors MAFF and KLF5 in renal fibrosis.","authors":"Meihui Deng, Xiao Tan, Xiaojie Peng, Weimin Zheng, Rui Fu, Shanshan Tao","doi":"10.1080/0886022X.2024.2415517","DOIUrl":"10.1080/0886022X.2024.2415517","url":null,"abstract":"<p><strong>Aim: </strong>This study explored the effect and mechanism of MAFF and HDAC6 on renal fibrosis and inflammation in lupus nephritis (LN).</p><p><strong>Methods: </strong>IL-33 treated renal epithelial cells and MRL/lpr mice were respectively used for <i>in vitro</i> and <i>in vivo</i> experiments. The expressions of HDAC6, MAFF, and KLF5 were measured in cells and renal tissues. Before and after cell transfection, the morphological changes in renal tissues were observed using Hematoxylin and eosin (H&E) and Masson staining. The proteinuria, serum creatinine (SCr), blood urea nitrogen (BUN), and double-stranded DNA (dsDNA) levels were detected by biochemical analysis. The expressions of fibrosis and inflammation related proteins (including α-SMA, Vimentin, IL-1β, IL-6, and TNF-α), p65, and iNOS were also detected. The relationship among MAFF, HDAC6, and KLF5 was determined by chromatin immunoprecipitation and dual luciferase reporter gene assay.</p><p><strong>Results: </strong>Renal tissues and cell models had elevated expressions of HDAC6 and KLF5, and decreased MAFF expression. HDAC6 suppression or MAFF overexpression led to suppression of proteinuria, SCr, BUN, and dsDNA levels, as well as attenuation of inflammatory infiltration and collagen deposition. HDAC6 can suppress MAFF expression <i>via</i> deacetylation to abolish its suppression of KLF5 expression, thus increasing KLF5 expression. <i>In vivo</i> and <i>in vitro</i> experiments showed the suppressive effect of HDAC6 suppression on renal fibrosis and inflammation can be abolished by KLF5 overexpression.</p><p><strong>Conclusion: </strong>HDAC6 suppresses MAFF expression <i>via</i> deacetylation to elevate KLF5 expression, which consequently enhances fibrosis and inflammatory response in LN.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11485742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sulforaphane regulates AngII-induced podocyte oxidative stress injury through the Nrf2-Keap1/ho-1/ROS pathway. 红豆杉通过Nrf2-Keap1/ho-1/ROS途径调节AngII诱导的荚膜氧化应激损伤
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-17 DOI: 10.1080/0886022X.2024.2416937
Wen Lu

Objective: This study aimed to investigate the therapeutic effects of sulforaphane and the role of the Nrf2-Keap1/HO-1/ROS pathway in AngII-induced oxidative stress in podocyte injury.

Methods: Mouse mpc5 podocytes were divided into four groups: control (Con), AngII, AngII + sulforaphane (AngII + SFN), and control + sulforaphane (Con + SFN). Western blotting was used to detect protein expression of Nrf2-Keap1, antioxidant enzyme HO-1, and apoptosis-related proteins. ROS levels were measured using a ROS assay kit, and cell survival and viability were assayed using the CCK-8 kit. Molecular interactions between Nrf2 and sulforaphane were analyzed computationally.

Results: Compared with the Con group, podocytes treated with AngII alone exhibited inhibited proliferation, reduced cell viability, lower Bcl-2 expression, and higher cleaved caspase 3 expression. In the presence of sulforaphane, AngII group showed a mild inhibition on podocyte proliferation but did not induce the aforementioned changes in Bcl-2 and cleaved caspase 3 expression. Similarly, compared to the Con group, AngII treatment alone had lower Nrf2 expression and higher Keap1 expression in podocytes, accompanied by a significant decrease in ROS content. However, in the presence of sulforaphane, AngII failed to induce increases in Nrf2 and a decrease in Keap1 expression, as well as ROS levels. Furthermore, cells treated with sulforaphane exhibited higher HO-1 levels than control cells, and co-incubation with AngII did not alter HO-1 levels. Computational modeling revealed hydrophobic interactions between sulforaphane and the amino acid LYS-462 of Nrf2, as well as hydrogen bonding with amino acid HIS-465. The binding score between sulforaphane and Nrf2 was -4.7.

Conclusion: Sulforaphane alleviated AngII-induced podocyte oxidative stress injury via the Nrf2-Keap1/HO-1/ROS pathway, providing new insights into therapeutic compounds for mitigating chronic kidney disease.

研究目的本研究旨在探讨莱菔硫烷的治疗作用以及 Nrf2-Keap1/HO-1/ROS 通路在 AngII 诱导的荚膜细胞氧化应激损伤中的作用:方法:将小鼠mpc5荚膜细胞分为四组:对照组(Con)、AngⅡ组、AngⅡ+苜蓿素组(AngⅡ+SFN)和对照组+苜蓿素组(Con+SFN)。用 Western 印迹法检测 Nrf2-Keap1、抗氧化酶 HO-1 和细胞凋亡相关蛋白的表达。使用 ROS 检测试剂盒测量 ROS 水平,使用 CCK-8 试剂盒检测细胞存活率和活力。通过计算分析了 Nrf2 与红豆杉素之间的分子相互作用:结果:与 Con 组相比,单用 AngII 处理的荚膜细胞增殖受抑制,细胞存活率降低,Bcl-2 表达降低,裂解 Caspase 3 表达升高。在莱菔硫烷存在的情况下,AngII 组对荚膜细胞增殖有轻微抑制作用,但并未引起上述 Bcl-2 和裂解 Caspase 3 表达的变化。同样,与 Con 组相比,单独 AngII 处理组的荚膜细胞中 Nrf2 表达较低,Keap1 表达较高,同时 ROS 含量显著降低。然而,在有莱菔硫烷存在的情况下,AngII 不能诱导 Nrf2 表达的增加和 Keap1 表达的降低,也不能诱导 ROS 含量的降低。此外,与对照细胞相比,用莱菔硫烷处理过的细胞表现出更高的 HO-1 水平,而与 AngII 共同作用也不会改变 HO-1 的水平。计算模型显示,莱菔硫烷与Nrf2的氨基酸LYS-462之间存在疏水相互作用,并与氨基酸HIS-465之间存在氢键作用。结论:结论:红景天通过Nrf2-Keap1/HO-1/ROS途径缓解了AngⅡ诱导的荚膜氧化应激损伤,为治疗慢性肾病提供了新的思路。
{"title":"Sulforaphane regulates AngII-induced podocyte oxidative stress injury through the Nrf2-Keap1/ho-1/ROS pathway.","authors":"Wen Lu","doi":"10.1080/0886022X.2024.2416937","DOIUrl":"10.1080/0886022X.2024.2416937","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the therapeutic effects of sulforaphane and the role of the Nrf2-Keap1/HO-1/ROS pathway in AngII-induced oxidative stress in podocyte injury.</p><p><strong>Methods: </strong>Mouse mpc5 podocytes were divided into four groups: control (Con), AngII, AngII + sulforaphane (AngII + SFN), and control + sulforaphane (Con + SFN). Western blotting was used to detect protein expression of Nrf2-Keap1, antioxidant enzyme HO-1, and apoptosis-related proteins. ROS levels were measured using a ROS assay kit, and cell survival and viability were assayed using the CCK-8 kit. Molecular interactions between Nrf2 and sulforaphane were analyzed computationally.</p><p><strong>Results: </strong>Compared with the Con group, podocytes treated with AngII alone exhibited inhibited proliferation, reduced cell viability, lower Bcl-2 expression, and higher cleaved caspase 3 expression. In the presence of sulforaphane, AngII group showed a mild inhibition on podocyte proliferation but did not induce the aforementioned changes in Bcl-2 and cleaved caspase 3 expression. Similarly, compared to the Con group, AngII treatment alone had lower Nrf2 expression and higher Keap1 expression in podocytes, accompanied by a significant decrease in ROS content. However, in the presence of sulforaphane, AngII failed to induce increases in Nrf2 and a decrease in Keap1 expression, as well as ROS levels. Furthermore, cells treated with sulforaphane exhibited higher HO-1 levels than control cells, and co-incubation with AngII did not alter HO-1 levels. Computational modeling revealed hydrophobic interactions between sulforaphane and the amino acid LYS-462 of Nrf2, as well as hydrogen bonding with amino acid HIS-465. The binding score between sulforaphane and Nrf2 was -4.7.</p><p><strong>Conclusion: </strong>Sulforaphane alleviated AngII-induced podocyte oxidative stress injury <i>via</i> the Nrf2-Keap1/HO-1/ROS pathway, providing new insights into therapeutic compounds for mitigating chronic kidney disease.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11488169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142473309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved survival prediction for kidney transplant outcomes using artificial intelligence-based models: development of the UK Deceased Donor Kidney Transplant Outcome Prediction (UK-DTOP) Tool. 利用基于人工智能的模型改进肾移植结果的存活率预测:开发英国死亡捐献者肾移植结果预测(UK-DTOP)工具。
IF 3 3区 医学 Q1 UROLOGY & NEPHROLOGY Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI: 10.1080/0886022X.2024.2373273
Hatem Ali, Arun Shroff, Karim Soliman, Miklos Z Molnar, Adnan Sharif, Bernard Burke, Sunil Shroff, David Briggs, Nithya Krishnan

The UK Deceased Donor Kidney Transplant Outcome Prediction (UK-DTOP) Tool, developed using advanced artificial intelligence (AI), significantly enhances the prediction of outcomes for deceased-donor kidney transplants in the UK. This study analyzed data from the UK Transplant Registry (UKTR), including 29,713 transplant cases between 2008 and 2022, to assess the predictive performance of three machine learning models: XGBoost, Random Survival Forest, and Optimal Decision Tree. Among these, XGBoost demonstrated exceptional performance with the highest concordance index of 0.74 and an area under the curve (AUC) consistently above 0.73, indicating robust discriminative ability and calibration. In comparison to the traditional Kidney Donor Risk Index (KDRI), which achieved a lower concordance index of 0.57, the UK-DTOP model marked a significant improvement, underscoring its superior predictive accuracy. The advanced capabilities of the XGBoost model were further highlighted through calibration assessments using the Integrated Brier Score (IBS), showing a score of 0.14, indicative of precise survival probability predictions. Additionally, unsupervised learning via k-means clustering was employed to identify five distinct clusters based on donor and transplant characteristics, uncovering nuanced insights into graft survival outcomes. These clusters were further analyzed using Bayesian Cox regression, which confirmed significant survival outcome variations across the clusters, thereby validating the model's effectiveness in enhancing risk stratification. The UK-DTOP tool offers a comprehensive decision-support system that significantly refines pre-transplant decision-making. The study's findings advocate for the adoption of AI-enhanced tools in healthcare systems to optimize organ matching and transplant success, potentially guiding future developments in global transplant practices.

利用先进的人工智能(AI)开发的英国死亡供体肾移植结果预测(UK-DTOP)工具大大提高了英国死亡供体肾移植的结果预测能力。这项研究分析了英国移植登记处(UKTR)的数据,包括2008年至2022年间的29713例移植病例,以评估三种机器学习模型的预测性能:XGBoost、随机生存森林和最优决策树。其中,XGBoost 表现优异,一致性指数最高,达到 0.74,曲线下面积 (AUC) 始终高于 0.73,显示出强大的判别能力和校准能力。传统的肾脏捐献者风险指数 (KDRI) 的一致性指数较低,仅为 0.57,与之相比,UK-DTOP 模型的一致性指数有了显著提高,凸显了其卓越的预测准确性。通过使用综合布赖尔评分(IBS)进行校准评估,XGBoost 模型的先进功能得到进一步凸显。此外,通过 k-means 聚类进行无监督学习,根据捐献者和移植特征确定了五个不同的聚类,从而揭示了移植物存活结果的细微差别。利用贝叶斯考克斯回归法对这些聚类进行了进一步分析,结果证实各聚类的存活结果存在显著差异,从而验证了该模型在加强风险分层方面的有效性。UK-DTOP 工具提供了一个全面的决策支持系统,大大改进了移植前的决策。研究结果倡导在医疗系统中采用人工智能增强型工具,以优化器官配型和移植成功率,从而为全球移植实践的未来发展提供潜在指导。
{"title":"Improved survival prediction for kidney transplant outcomes using artificial intelligence-based models: development of the UK Deceased Donor Kidney Transplant Outcome Prediction (UK-DTOP) Tool.","authors":"Hatem Ali, Arun Shroff, Karim Soliman, Miklos Z Molnar, Adnan Sharif, Bernard Burke, Sunil Shroff, David Briggs, Nithya Krishnan","doi":"10.1080/0886022X.2024.2373273","DOIUrl":"https://doi.org/10.1080/0886022X.2024.2373273","url":null,"abstract":"<p><p>The UK Deceased Donor Kidney Transplant Outcome Prediction (UK-DTOP) Tool, developed using advanced artificial intelligence (AI), significantly enhances the prediction of outcomes for deceased-donor kidney transplants in the UK. This study analyzed data from the UK Transplant Registry (UKTR), including 29,713 transplant cases between 2008 and 2022, to assess the predictive performance of three machine learning models: XGBoost, Random Survival Forest, and Optimal Decision Tree. Among these, XGBoost demonstrated exceptional performance with the highest concordance index of 0.74 and an area under the curve (AUC) consistently above 0.73, indicating robust discriminative ability and calibration. In comparison to the traditional Kidney Donor Risk Index (KDRI), which achieved a lower concordance index of 0.57, the UK-DTOP model marked a significant improvement, underscoring its superior predictive accuracy. The advanced capabilities of the XGBoost model were further highlighted through calibration assessments using the Integrated Brier Score (IBS), showing a score of 0.14, indicative of precise survival probability predictions. Additionally, unsupervised learning <i>via</i> k-means clustering was employed to identify five distinct clusters based on donor and transplant characteristics, uncovering nuanced insights into graft survival outcomes. These clusters were further analyzed using Bayesian Cox regression, which confirmed significant survival outcome variations across the clusters, thereby validating the model's effectiveness in enhancing risk stratification. The UK-DTOP tool offers a comprehensive decision-support system that significantly refines pre-transplant decision-making. The study's findings advocate for the adoption of AI-enhanced tools in healthcare systems to optimize organ matching and transplant success, potentially guiding future developments in global transplant practices.</p>","PeriodicalId":20839,"journal":{"name":"Renal Failure","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Renal Failure
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1