Regenerative Therapy最新文献

{"title":"Low-dose lipopolysaccharide pretreatment enhanced the proliferation and antibacterial activity of human adipose-derived mesenchymal stem cells","authors":"Linling Li ,&nbsp;Jielin Diao ,&nbsp;Feng Wang ,&nbsp;Xia Wang ,&nbsp;Yicai Liu ,&nbsp;Xiaoming Fu","doi":"10.1016/j.reth.2025.101057","DOIUrl":"10.1016/j.reth.2025.101057","url":null,"abstract":"<div><h3>Introduction</h3><div>Adipose-derived mesenchymal stem cells (ADSCs) have been widely investigated for their pro-angiogenic and immunomodulatory roles in the repair of infected wounds. However, the direct antimicrobial effects of ADSCs and the underlying regulatory mechanisms remain poorly characterized. In particular, the functional modulation of ADSCs by low-dose lipopolysaccharide (LPS) preconditioning has not been systematically investigated.</div></div><div><h3>Methods</h3><div>We evaluated the effects of LPS preconditioning on the proliferation and apoptosis of human ADSCs (hADSCs), as well as the antimicrobial activity and wound-healing potential of hADSC-conditioned medium (hADSC-CM).</div></div><div><h3>Results</h3><div>Analysis demonstrated that at concentrations ranging from 10 to 500 ng/mL, LPS significantly enhanced the proliferation of hADSCs, with the highest viability observed at 500 ng/mL and no evidence of increased apoptosis. Moreover, LPS preconditioning markedly upregulated the expression of antimicrobial peptides (LL-37 and HBD-2) in hADSC-CM, leading to improved inhibition of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> growth. In vivo experiments further confirmed that 500 ng/mL of LPS-hADSC-CM significantly accelerated the healing of infected wounds, increased collagen deposition, and downregulated the expression of iNOS, thus suggesting enhanced inflammation resolution and tissue regeneration.</div></div><div><h3>Conclusion</h3><div>These findings highlight the capacity of LPS preconditioning to potentiate the biological functions of hADSCs, enhancing the antimicrobial and regenerative efficacy of hADSC-CM, and providing a promising strategy for the treatment of chronically infected wounds.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101057"},"PeriodicalIF":3.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best evidence summary for platelet-rich plasma treatment of chronic wounds","authors":"Xinru Zhang ,&nbsp;Xingxing Zhang ,&nbsp;Luxin Wang ,&nbsp;Li Zhen ,&nbsp;Meiyan Lin ,&nbsp;Yanan Li ,&nbsp;Lihong Gong ,&nbsp;Haiting Zeng ,&nbsp;Weiqing Ruan ,&nbsp;Mulan Zhu","doi":"10.1016/j.reth.2025.101053","DOIUrl":"10.1016/j.reth.2025.101053","url":null,"abstract":"<div><h3>Objective</h3><div>To summarize the best evidence for platelet-rich plasma therapy in chronic wounds, providing an evidence-based foundation for standardizing its clinical practice.</div></div><div><h3>Methods</h3><div>Guided by the “6S” evidence pyramid model, we systematically searched 12 databases including Cochrane Library and Pubmed, 9 guideline websites including Guidelines International Network (GIN) and National Institute for Health and Clinical Excellence (NICE), and 10 professional websites including World Union of Wound Healing Societies (WUWHS), for relevant evidence from the establishment of the database to May 1, 2025. Two researchers independently conducted quality assessment, evidence extraction, and integration of the included literature.</div></div><div><h3>Results</h3><div>A total of 17 articles were included, comprising 3 guidelines, 5 expert consensus statements, and 9 systematic reviews. The evidence was categorized into six key treatment domains: application principles, indications and contraindications, pre-treatment preparations, treatment protocols, efficacy monitoring, and management strategies. 27 individual recommendations were derived from these categories.</div></div><div><h3>Conclusion</h3><div>Platelet-rich plasma therapy can be used as an adjunctive treatment for the management of chronic wounds. Clinicians and wound care specialists should thoroughly assess the applicability and timing of platelet-rich plasma, considering the specific clinical context, and combine it with the patient's physical condition and preferences for clinical application, promoting chronic wound healing and reducing the global disease burden of chronic wounds.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101053"},"PeriodicalIF":3.5,"publicationDate":"2025-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of local administration of microRNA-31/210 on bone regeneration surrounding hydroxyapatite/tricalcium phosphate -coated titanium implant in an ovariectomized rat model","authors":"Shinichi Ueki ,&nbsp;Takeshi Shoji ,&nbsp;Hideki Saka ,&nbsp;Hiroki Kaneta ,&nbsp;Hiroyuki Morita ,&nbsp;Yosuke Kozuma ,&nbsp;Nobuo Adachi","doi":"10.1016/j.reth.2025.101055","DOIUrl":"10.1016/j.reth.2025.101055","url":null,"abstract":"<div><h3>Background</h3><div>With the aging population, the prevalence of total joint arthroplasty in older adults with compromised bone conditions, such as osteoporosis, is increasing, raising concerns on the initial fixation of implants and aseptic loosening. Recent studies have highlighted the potential of microRNAs (miRNAs) to enhance osteogenesis and angiogenesis, potentially improving implant osseointegration. This study aimed to identify miRNAs with the highest osteogenic and angiogenic potential in vitro, and evaluate its effects on implant osseointegration and surrounding bone regeneration in an ovariectomized (OVX) rat model.</div></div><div><h3>Methods</h3><div>In vitro studies were conducted to identify miRNAs exhibiting the greatest osteogenic and angiogenic potential among candidate miRNAs (miR-31, -34a, −146, −210, −218, and −31 + 210). Subsequently, the most effective miRNA was selected and locally administered to the bone matrix, where hydroxyapatite/tricalcium phosphate (HA/TCP)-coated titanium implants were placed in the femurs of OVX rats for in vivo studies. At 2, 4, and 8 weeks post-implantation, implant osseointegration, osteogenesis, angiogenesis of the matrix bone, and the initial fixation of the implant were evaluated using histological, genetic, radiological, and biomechanical assessments.</div></div><div><h3>Results</h3><div>miR-31 and miR-210 were strongly associated with osteogenesis, whereas miR-31 was strongly associated with angiogenesis. Moreover, the simultaneous administration of miR-31 and miR-210 resulted in the highest osteogenic potential among the miRNAs tested. In the OVX rat model, local administration of miR-31 + 210 significantly enhanced implant osseointegration, osteogenesis, angiogenesis within the bone matrix, and initial fixation of the implant compared to controls.</div></div><div><h3>Conclusion</h3><div>Local administration of miR-31 + 210 around HA/TCP-coated implants effectively improved implant osseointegration, the bone matrix environment, and initial fixation of implants in osteoporotic bone, likely by promoting osteogenesis and angiogenesis. This strategy holds promise as a novel regeneration therapy for enhancing implant fixation in patients with poor bone quantity.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101055"},"PeriodicalIF":3.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in immunomodulation-based strategies for bone repair","authors":"Wang Yuqiang,&nbsp;Zhang Ziyan,&nbsp;Sun Xuedi,&nbsp;Piao Chengdong","doi":"10.1016/j.reth.2025.101054","DOIUrl":"10.1016/j.reth.2025.101054","url":null,"abstract":"<div><div>Bone regeneration is a highly coordinated process shaped by the interplay between immune responses and osteogenic mechanisms. Immune cells such as neutrophils, macrophages, T cells, and B cells dynamically regulate the local microenvironment through cytokine secretion and signaling pathways, thereby influencing osteogenesis, angiogenesis and bone remodeling, while dysregulated or prolonged inflammation can disrupt healing. Growing evidence has highlighted the potential of leveraging immunomodulation to enhance bone repair. This review synthesizes recent progress in immunoregulatory strategies by comparing cellular therapies, molecular interventions and biomaterial-based approaches in terms of their mechanisms, their effects on osteogenesis and angiogenesis, and their translational potential. Particular emphasis is placed on immune cell specific signaling pathways, biomaterial design parameters including surface topography, porosity, ion release and stiffness, and emerging technologies such as immune responsive hydrogels, programmable scaffolds and exosome based delivery systems. Current findings indicate that mesenchymal stem cells and regulatory T cells not only provide progenitor sources but also reshape the immune milieu through paracrine factors and exosomes; cytokines, small molecules, microRNAs and pro resolving mediators effectively modulate inflammatory cascades to promote vascularized bone formation; and immunomodulatory biomaterials enable spatiotemporal regulation of macrophage polarization, particularly the transition from the pro inflammatory M1 phenotype to the reparative M2 phenotype. Collectively, these advances highlight that bone repair is fundamentally an immunologically driven process, and integrating temporal immune regulation with emerging therapeutic platforms offers a promising pathway toward precise and personalized bone regeneration.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101054"},"PeriodicalIF":3.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145839205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a digital analysis system for a novel 3D culture-based colony formation to detect malignantly transformed cells in human cell-based therapeutic products","authors":"Shinji Kusakawa ,&nbsp;Tingshu Yang ,&nbsp;Rumi Sawada ,&nbsp;Ryuji Kato ,&nbsp;Yoji Sato ,&nbsp;Satoshi Yasuda","doi":"10.1016/j.reth.2025.101052","DOIUrl":"10.1016/j.reth.2025.101052","url":null,"abstract":"<div><h3>Introduction</h3><div>The presence of malignantly transformed cells in human cell-based therapeutic products (hCTPs) is a significant safety concern. Although such cellular impurities in hCTPs can be assessed by detecting anchorage-independent growth using conventional soft agar colony formation (SACF) assays, the sensitivity of these assays is often insufficient. To overcome this limitation, we previously developed a novel tumorigenicity-associated testing method, the digital SACF (D-SACF) assay, which combines a partitioned culture of test cells to concentrate target cells with colony detection via image analysis. However, conventional soft agar culture involves complicated operations, such as preparing multilayered agar media and temperature control, and further technical optimization is required for the widespread adoption of the D-SACF assay.</div></div><div><h3>Methods</h3><div>In this study, we focused on a new culture system incorporating a three-dimensional (3D) culture method using a liquid medium containing the low-molecular-weight agar polymer LA717 in low-adhesion culture vessels. We initially confirmed conditions for the efficient high-density 3D culture of normal cells using LA717-supplemented medium in low-adhesion 96-well plates.</div></div><div><h3>Results</h3><div>Using human mesenchymal stem/stromal cells (MSCs) as a normal cell model and HeLa cells as a transformed cell model, we demonstrated that the new 3D culture system effectively maintained the dispersion of MSCs and prevented their aggregation, while transformed HeLa cells exhibited robust anchorage independence, thereby establishing the new liquid/low-molecular-weight agar colony formation (LACF) method as an alternative to SACF.</div></div><div><h3>Conclusions</h3><div>Finally, by systematizing the digital analysis system for the LACF assay (D-LACF assay), which streamlines the overall workflow from the performance evaluation of the test method to product testing and result interpretation, the limitations of the conventional soft agar-based D-SACF assay were addressed, and its practicality and utility were enhanced. This <em>in vitro</em> evaluation system is expected to provide a promising approach for improving the quality and safety of hCTPs.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101052"},"PeriodicalIF":3.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145796775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NSUN2-engineered human umbilical cord mesenchymal stem cell-derived exosomes ameliorate tendon injury by promoting DNM2 expression","authors":"Yuxin Liu ,&nbsp;Yalu Pu ,&nbsp;Qi Li ,&nbsp;Liang Yu ,&nbsp;Hailong Kou ,&nbsp;Yang Liu ,&nbsp;Zhizhong Shen ,&nbsp;Yilei Zhao","doi":"10.1016/j.reth.2025.101050","DOIUrl":"10.1016/j.reth.2025.101050","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Tendon injuries are a common musculoskeletal problem, often leading to chronic pain and disability. Current treatment options, including surgical interventions and physical therapy, have limitations in terms of efficacy and potential complications. Human umbilical cord mesenchymal stem cells (HUCMSCs) are a promising source of mesenchymal stem cells (MSCs), and exosomes derived from HUCMSCs have been shown to mediate various biological processes. This study aims to investigate the role of HUCMSC-derived exosomes in tendon injuries and the underlying mechanism.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Exosomes were isolated from HUCMSCs using differential centrifugation. Cell viability was assessed using a cell counting kit-8 assay. Cell proliferation was measured by a 5-Ethynyl-2′-deoxyuridine assay. Transwell invasion assays were conducted to analyze cell invasion, and wound-healing assays were used to evaluate cell migration. Quantitative real-time PCR (qRT-PCR) was employed to analyze DNM2 mRNA expression. Western blotting was used to detect the protein expression of NOP2/Sun RNA methyltransferase 2 (NSUN2), C cluster of differentiation 63 (CD63), CD81, and dynamin 2 (DNM2). m5C methylated RNA immunoprecipitation and RNA immunoprecipitation (RIP) assays were performed to analyze the association of NSUN2 with DNM2. Additionally, an RIP assay was conducted to study the interaction among Y-box binding protein 1 (YBX1), NSUN2, and DNM2 in injured tenocytes. Rats were subjected to superficial tendon excision and partial transection of the deep Achilles tendon to induce tendon injury. Hematoxylin and eosin (HE) staining was used to analyze the pathological conditions of Achilles tendon tissues, and an immunohistochemistry (IHC) assay was performed to detect the positive expression rates of NSUN2 protein.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;HUCMSC-derived exosomes significantly promoted the proliferation, migration, and invasion of injured tenocytes. Overexpression of NSUN2 also enhanced the proliferative, migratory and invasive abilities of injured tenocytes. The exosomes derived from NSUN2-overexpressing HUCMSCs showed a more pronounced promoting effect on injured tenocyte proliferation, migration, and invasion compared to control exosomes. NSUN2 stabilized DNM2 mRNA expression through m5C methylation modification. YBX1 interacted with NSUN2 to stabilize DNM2 expression. In addition, knockdown of DNM2 attenuated the promoting effects of HUCMSC-derived exosomes with NSUN2 overexpression on the proliferation, migration, and invasion of injured tenocytes. Moreover, exosomes derived from NSUN2-overexpressing HUCMSCs improved tendon injury in a rat model, as indicated by enhanced pathological conditions within the tendon tissues.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;HUCMSC-derived exosomal NSUN2 played a crucial role in ameliorating tendon injury by promoting DNM2 expression. The findings suggest that exosomes from NSUN2-ove","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101050"},"PeriodicalIF":3.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145796776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PET/EVOH nonwoven fabrics support in vitro expansion of mesenchymal stem cells with high differentiation potential","authors":"Yu-Min Chen ,&nbsp;Chihoko Tokoda ,&nbsp;Yasuhiko Tabata","doi":"10.1016/j.reth.2025.101046","DOIUrl":"10.1016/j.reth.2025.101046","url":null,"abstract":"<div><div>Cell expansion under three-dimensional (3D) condition has been shown to better preserve cellular properties by mimicking the native microenvironment, thereby creating a more physiologically relevant culture system. This study investigated the capacity of a three-dimensional nonwoven polyethylene terephthalate (PET)/ethylene vinyl alcohol (EVOH) scaffold as a 3D substrate for large-scale expansion of mesenchymal stem cells (MSC). A seven-fold increase in cell number was observed after 14 days of cultivation, and cells were well distributed with an efficient infiltration within scaffolds based on the hematoxylin and eosin (H&amp;E) staining. A high cell survival and pertaining of metabolic activity were demonstrated by live/dead and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) stainings, respectively. The preservation of MSC properties was confirmed by their differentiation potential toward osteogenic, adipogenic, and chondrogenic lineages. Both <em>in situ</em> differentiation of cell-expanded scaffolds and the subsequent differentiation after cell retrieving from scaffolds revealed the successful responsiveness of expanded MSC to lineage-specific stimuli. These findings suggest the potential of this PET/EVOH scaffold as the 3D culture substrate enabling efficient MSC proliferation while maintaining key functional properties.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101046"},"PeriodicalIF":3.5,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lubricin maintains temporomandibular joint homeostasis by regulating synovial inflammation","authors":"Soichiro Negishi ,&nbsp;Kazuhiro Shibusaka ,&nbsp;Miki Maemura ,&nbsp;Masayuki Tsukasaki ,&nbsp;Seigo Ohba ,&nbsp;Sakae Tanaka ,&nbsp;Taku Saito ,&nbsp;Yutaka Suzuki ,&nbsp;Hiroyuki Okada ,&nbsp;Fumiko Yano","doi":"10.1016/j.reth.2025.101051","DOIUrl":"10.1016/j.reth.2025.101051","url":null,"abstract":"<div><h3>Introduction</h3><div>Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative joint disease characterized by cartilage degeneration, synovial inflammation, and subchondral bone remodeling, yet its molecular pathogenesis remains poorly understood. In this study, we investigated the role of proteoglycan 4 (<em>Prg4</em>), also known as lubricin, in maintaining temporomandibular joint (TMJ) homeostasis under physiological and pathological conditions, with the aim of exploring its potential for regenerative therapeutic applications.</div></div><div><h3>Methods</h3><div>Using high-resolution Visium HD spatial transcriptomics, we examined the spatial distribution of <em>Prg4</em> expression within the TMJ. To model TMJ-OA, surgically induced anterior disc displacement (ADD) was performed in wild-type (WT) and <em>Prg4</em>-knockout (Prg4-KO) mice. In addition, inflammatory stimulation with IL-1β was applied to synovial cells <em>in vitro</em>. Lineage tracing approaches were used to track <em>Prg4</em>-expressing cells under pathological conditions.</div></div><div><h3>Results</h3><div>Spatial transcriptomics revealed that <em>Prg4</em> expression was highly localized to the posterior synovium of the articular disc, with markedly lower expression in anterior regions. While sham-operated TMJs remained histologically intact, the ADD model resulted in condylar deformation, cartilage degeneration, synovial hyperplasia, and subchondral bone loss—phenotypes that were significantly exacerbated in Prg4-KO mice. Furthermore, IL-1β stimulation increased matrix metalloproteinase expression in <em>Prg4</em>-deficient synovial cells. Lineage tracing demonstrated expansion of <em>Prg4</em>-expressing cells within inflamed synovial tissues in the ADD model. Quantitative analysis revealed that <em>Prg4</em> expression was transiently increased at 2 weeks after ADD induction and returned to control levels by 8 weeks, indicating a time-dependent regulatory role during inflammation.</div></div><div><h3>Conclusion</h3><div>These findings highlight the region-specific and time-dependent function of <em>Prg4</em> in the TMJ and underscore its critical role in suppressing joint inflammation and degeneration. Importantly, our results suggest that modulation of <em>Prg4</em> expression or lubricin supplementation could serve as a regenerative therapeutic strategy for preserving TMJ homeostasis and preventing chronic degenerative progression, providing a promising avenue for clinical translation in TMJ-OA treatment.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101051"},"PeriodicalIF":3.5,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qianzheng powder promotes facial nerve regeneration via BDNF/TrkB/CREB pathway activation","authors":"Liang Chen,&nbsp;Chaoqun Wang,&nbsp;Lixin Jiang,&nbsp;Qiang Xie,&nbsp;Hongru Qin,&nbsp;Ziheng Zhang","doi":"10.1016/j.reth.2025.101048","DOIUrl":"10.1016/j.reth.2025.101048","url":null,"abstract":"<div><h3>Introduction</h3><div>Facial nerve injury (FNI) is a common peripheral neuropathy that severely impairs facial function and quality of life. Qianzheng Powder (QZP) is a traditional Chinese herbal formula used to treat facial paralysis clinically, yet its neuroprotective mechanisms remain unclear. This study aims to evaluate the therapeutic effects of QZP on FNI and potential underlying mechanisms.</div></div><div><h3>Methods</h3><div>A FNI model was established in male C57BL/6 mice by performing facial nerve crush surgery. QZP (3.51 g/kg) was administered orally once daily for 14 days post-surgery. Facial function was assessed behaviorally. Tissue samples were collected on day 21 for histological evaluation, qPCR and Western blotting. Liver and kidney safety were also assessed via H&amp;E staining and serum biochemical markers.</div></div><div><h3>Results</h3><div>QZP significantly improved facial motor function from day 7 post-injury. Additionally, QZP treatment mitigated neuronal loss in the facial motor nucleus, attenuated buccinator muscle atrophy, and enhanced myelin regeneration, as evidenced by increased MPZ and MBP expression. These were consistent with the increace of the BDNF, TrkB, and <em>p</em>-CREB/CREB expressions in QZP-treated mice. No hepatic or renal toxicity was detected.</div></div><div><h3>Conclusion</h3><div>QZP promotes structural and functional recovery of facial nerve following injury, likely through activation of the BDNF/TrkB/CREB axis, and demonstrates a favorable safety profile. These findings support its potential as a therapeutic adjunct in peripheral nerve repair.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101048"},"PeriodicalIF":3.5,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of donor–recipient age differences in allogenic rat thyroid transplantation","authors":"Ayumi Arauchi ,&nbsp;Katsuhisa Matsuura ,&nbsp;Tatsuya Shimizu","doi":"10.1016/j.reth.2025.101047","DOIUrl":"10.1016/j.reth.2025.101047","url":null,"abstract":"<div><h3>Introduction</h3><div>Regenerative medicine for tissue dysplasia, hypoplasia, and functional impairment of tissues and cells has advanced considerably, and thyroid disease is no exception. Inducing differentiation of thyroid cells from patient-derived cells and transplanting them back into patients is an ideal approach because it eliminates the need for immunosuppressive drugs. However, the relationship between the maturity of cells or tissues derived from various sources and engraftment outcomes remains unclear. In this study, we evaluated the effect of donor–recipient age differences using thyroids from living rat donors, given the current lack of sufficiently mature stem cell-derived thyroid tissue.</div></div><div><h3>Methods</h3><div>Histological and gene expression differences were analyzed in thyroids of retired rats (&gt;20 weeks old with impaired reproductive function) and 3-week-old rats. Thyroid transplantation experiments were conducted between age-matched or age-mismatched groups. Donor thyroids were implanted beneath the renal capsule of recipient rats without total thyroidectomy, and grafts from retired rats transplanted into 3-week-old rats were resected 1 and 6 months post-transplantation, while others were resected 1 month post-transplantation for immunohistological analysis and gene expression analysis of thyroid differentiation markers.</div></div><div><h3>Results</h3><div>Thyroids from 3-week-old rats and retired rats showed minimal histological and functional differences; however, the expression levels of several thyroid-specific marker genes were significantly higher in retired rats. When thyroids were transplanted between age-matched donors and recipients, clear engraftment was observed at 1 month. Although robust engraftment was also observed when thyroids from retired rats were transplanted into 3-week-old recipients at both 1 and 6 months, transplantation of thyroids from 3-week-old donors into retired rats resulted in disrupted follicular structures and fibrotic changes at 1 month. In contrast, mRNA expression levels of transplanted thyroids presented no significant differences between age-matched and age-mismatched transplantation.</div></div><div><h3>Conclusions</h3><div>Mismatch between donor thyroid maturity and recipient age may influence engraftment in rat allogenic thyroid transplantation.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"31 ","pages":"Article 101047"},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145691074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}