Regenerative Therapy最新文献

{"title":"Objective evaluation of platelet-rich plasma mesotherapy on facial skin quality: An interim split-face analysis using an advanced imaging system","authors":"Masashi Umeda,&nbsp;Takahiko Tamura,&nbsp;Kohki Okumura,&nbsp;Reiko Kiuchi,&nbsp;Hiroo Teranishi","doi":"10.1016/j.reth.2025.10.008","DOIUrl":"10.1016/j.reth.2025.10.008","url":null,"abstract":"<div><h3>Introduction</h3><div>Autologous platelet-rich plasma (PRP), which is rich in growth factors, is widely used in aesthetic medicine to improve facial skin quality. However, its efficacy has been largely supported by subjective evaluations and case reports; objective and quantitative evidence remains limited. This study aimed to objectively and quantitatively evaluate the effects of PRP mesotherapy on facial skin quality, using a validated image analysis system.</div></div><div><h3>Methods</h3><div>Nine healthy adults (six females and three males; median age, 34 years) employed at the Tokyo Chuo Beauty Clinic were enrolled in this study. A split-face design was used, with PRP injected into the left hemiface and saline injected into the right hemiface under identical conditions (5 mL each). Facial images were obtained at baseline, two weeks, and one month using the NeoVoir system, which automatically identifies ten facial regions and extracts multiple quantitative skin parameters. The percentage change from baseline was compared between the sides.</div></div><div><h3>Results</h3><div>The primary endpoint, the gloss area at one month, showed a significantly greater increase on the PRP-treated side than on the saline-treated side (median difference, 16.3 %; p = 0.0039). At two weeks, the gloss also improved more on the PRP side, although the difference was not significant after false discovery rate (FDR) correction. Pore scores showed a greater reduction on the PRP side at one month (median difference, −17.6 %; p = 0.0039), but this result did not remain statistically significant after FDR adjustment (q = 0.0663). No significant differences were observed in wrinkle area, melanin content, or pigmentation indices. No serious adverse events occurred; only transient, mild local reactions such as redness and swelling were noted on both sides.</div></div><div><h3>Conclusion</h3><div>This study provides novel objective evidence that PRP mesotherapy significantly improves facial gloss, a key feature of healthy young skin. An exploratory trend toward pore reduction was also observed, although mechanistic interpretations remain hypothesized owing to the absence of biophysical and structural measurements. Moving beyond subjective assessments, these findings support the potential of PRP for enhancing facial brightness, smoothness, and overall skin quality. Further studies with larger cohorts, extended follow-ups, and multimodal evaluations are warranted to validate and expand upon these preliminary results.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 969-976"},"PeriodicalIF":3.5,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145361010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfated hyaluronic acid for wound repair: Mechanisms, materials, and translational challenges","authors":"Min Lin ,&nbsp;Danni Zhou ,&nbsp;Kaixuan Chen ,&nbsp;Hankang Jiang ,&nbsp;Wenjie Chen ,&nbsp;Jing Wu ,&nbsp;Guizhi Zhu ,&nbsp;Kailei Xu ,&nbsp;Peng Wei","doi":"10.1016/j.reth.2025.10.006","DOIUrl":"10.1016/j.reth.2025.10.006","url":null,"abstract":"<div><div>Sulfated hyaluronic acid (sHA) is emerging as a promising biomaterial for advanced wound dressings because it combines the intrinsic biocompatibility and extracellular matrix (ECM)-like hydration of hyaluronic acid (HA) with added chemical functionality that enhances stability and bioactivity. Chronic wounds, particularly diabetic ulcer, —remain a major clinical challenge due to persistent inflammation, protease/reactive oxygen species (ROS)-rich microenvironments, impaired angiogenesis, and poor growth-factor retention; native HA is limited by rapid enzymatic degradation, low protein affinity, and potentially pro-inflammatory low-molecular fragments, motivating strategies to introduce sulfate groups onto HA to tune its interactions with cells and proteins. In this review, we performed a comprehensive literature review of the biological functions and applications of sHA in wound healing. Key results demonstrate that sHA significantly enhances anti-inflammatory responses, promotes sustained release of cationic therapeutics, improves angiogenesis, and reduces fibrosis. Furthermore, sHA-functionalized hydrogels accelerate diabetic wound healing by modulating macrophage polarization, enhancing re-epithelialization, and supporting vascularization. In further, we also offered a practical roadmap for researchers and clinicians to rationally design next-generation sHA-based dressings with improved reproducibility, controlled release, and safety.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 956-968"},"PeriodicalIF":3.5,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sevoflurane suppresses LPS-induced microglia injury by regulating USP11-mediated UHRF1 deubiquitination","authors":"Dongzhi Liu,&nbsp;Chengliang Sun,&nbsp;Xiuli Zhang,&nbsp;Shunheng Gao","doi":"10.1016/j.reth.2025.09.004","DOIUrl":"10.1016/j.reth.2025.09.004","url":null,"abstract":"<div><h3>Background</h3><div>Spinal cord injury (SCI) is a devastating neurological and pathological condition that leads to severe motor, sensory, and autonomic dysfunctions. The neuroprotective effect of Sevoflurane (Sevo) in the rat model of SCI has been reported. However, the mechanism of Sevo is still elusive.</div></div><div><h3>Methods</h3><div>24 rats were divided into four groups: sham group, sham + Sevo group, SCI group, and SCI + Sevo group. On days 0, 14, 28, and 42 post-SCI, functional recovery was evaluated using the BBB test. The histological changes in the spinal cord were observed by HE staining. Western blotting, ELISA, and corresponding kits were used to detect the effects of Sevo on apoptosis, inflammatory factors, and oxidative stress. Human microglia HMC3 were induced by lipopolysaccharide (LPS) to mimic the <em>in vitro</em> environment of SCI. Ubiquitin-specific peptidase 11 (USP11) and ubiquitin-like, containing PHD and RING finger domains 1 (UHRF1), B-cell lymphoma-2 (BCL-2), and Bcl-2 related X protein (BAX) protein levels were determined using Western blot. Cell apoptosis, Interleukin-1β (IL-1β), and IL-6 levels were assessed using flow cytometry and ELISA. malondialdehyde (MDA), glutathione (GSH), and Reactive oxygen species (ROS) products were examined using special assay kits. After Ubibrowser prediction, GO enrichment, and protein-protein interaction (PPI) networks, the interaction between USP11 and UHRF1 was verified using Co-immunoprecipitation (CoIP) assay.</div></div><div><h3>Results</h3><div>Sevo treatment improved spinal cord functional recovery in rats, as evidenced by enhanced BBB locomotor rating scale and neuron death <em>in vivo.</em> Meanwhile, Sevo also reduced inflammation and oxidative damage in rats following SCI. Sevo exposure decreased USP11 and UHRF1 expression in LPS-treated HMC3 cells. Sevo repressed LPS-triggered HMC3 cell apoptosis, inflammatory response, and oxidative stress promotion by regulating USP11. At the molecular level, USP11 interacted with UHRF1 and maintained its stabilization by removing ubiquitin.</div></div><div><h3>Conclusion</h3><div>Sevo could protect LPS-induced HMC3 cell apoptosis, inflammation, and oxidative stress by regulating the USP11/UHRF1 axis, which might provide a novel therapeutic mechanism for Sevo in SCI.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 946-955"},"PeriodicalIF":3.5,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting ER stress-mediated apoptosis by MSC-derived exosomes: A novel therapeutic strategy against pulmonary fibrosis","authors":"Ruixi Luo,&nbsp;Yaqiong Wei,&nbsp;La Wang,&nbsp;Peng Chen,&nbsp;Didong Lou,&nbsp;Weiyi Tian","doi":"10.1016/j.reth.2025.10.004","DOIUrl":"10.1016/j.reth.2025.10.004","url":null,"abstract":"<div><h3>Introduction</h3><div>Idiopathic pulmonary fibrosis (IPF) is marked by a gradual decline in pulmonary function over time and is associated with a grim prognosis. In the pathogenesis of IPF, persistent endoplasmic reticulum (ER) stress plays a significant role in promoting fibrosis through pathways involving apoptosis. Mesenchymal stem cell-derived exosomes (MSC-Ex) have shown promise in mitigating pulmonary fibrosis by inhibiting apoptosis. Nonetheless, the precise mechanisms underlying this effect remain unclear. In our previous findings, we demonstrated that MSCs alleviate pulmonary fibrosis by regulating ER stress. Building upon this, we sought to investigate whether MSC-Ex could mitigate alveolar epithelial cell apoptosis through the ER stress pathway. We posited that targeting ER stress could represent a crucial mechanism by which MSC-Ex alleviate apoptosis in IPF models.</div></div><div><h3>Methods and results</h3><div>In this study, bleomycin (BLM) induced apoptosis in A549 cells, and MSC-Ex treatment reduced apoptotic cells and the Bax/Bcl-2 ratio. ER stress is involved in BLM-induced apoptosis in A549 cells, and MSC-Ex reduced ER stress-related protein (Bip and CHOP) expression and reversed the morphological changes of the ER in A549 cells. Moreover, blockade of ER stress with ER stress inhibitor TUDCA contributed to the amelioration of apoptosis in A549 cells, indicating that MSC-Ex reduced BLM-induced apoptosis at least partly by modulating ER stress. <em>In vivo,</em> MSC-Ex injection decreased BLM-induced pulmonary fibrosis in mice, as well as ER stress and apoptosis in the lung tissues.</div></div><div><h3>Conclusions</h3><div>In conclusion, ER stress induced apoptosis in BLM-treated A549 cells, and MSC-Ex treatment mitigated apoptosis via inhibiting ER stress. This study provides a novel mechanism for MSC-Ex-mediated protection on apoptosis in an IPF model and suggests that MSC-Ex could be a promising therapeutic strategy for IPF.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 933-945"},"PeriodicalIF":3.5,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal mechanisms of platelet-rich plasma in bone defect repair: Angiogenesis, inflammation modulation, and metabolic regulation","authors":"Junjie Chen ,&nbsp;Bo Liu ,&nbsp;Jiayang He ,&nbsp;Yuhao Wei ,&nbsp;Yuhan He ,&nbsp;Hang Zhou ,&nbsp;Zhiqiang Zhang ,&nbsp;Yiwen Weng ,&nbsp;Ming Cheng","doi":"10.1016/j.reth.2025.09.007","DOIUrl":"10.1016/j.reth.2025.09.007","url":null,"abstract":"<div><div>Bone defect is the loss of bone tissue due to trauma, fracture, infection, etc. This loss may lead to non-union of fracture, bone defect or bone deficiency, which seriously affects the patient's quality of life and even leads to dysfunction and disability. Although surgical repair is a common treatment modality, the surgical procedure is complicated, with a long postoperative recovery period and certain surgical risks, such as infection and bleeding. Platelet-rich plasma (PRP), as a novel therapeutic approach, has demonstrated remarkable potential in bone defect repair. PRP is rich in growth factors, such as platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), etc., and can have a beneficial effect on bone defect repair through various mechanisms, such as the release of growth factors, the promotion of angiogenesis, the inhibition of inflammatory response, and the recruitment of activated cells and other mechanisms, which play a positive role in the repair of bone defects and provide strong support for the regeneration and repair of bone tissue.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 920-932"},"PeriodicalIF":3.5,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Transplantation of chemically induced liver progenitors in Nagase analbuminemic rats under liver regenerative stimulus’ [Regen Ther 30 (2025) 1–8]","authors":"Masayuki Fukumoto,&nbsp;Akihiko Soyama,&nbsp;Daisuke Miyamoto,&nbsp;Takanobu Hara,&nbsp;Hajime Matsushima,&nbsp;Hajime Imamura,&nbsp;Mampei Yamashita,&nbsp;Tomohiko Adachi,&nbsp;Kengo Kanetaka,&nbsp;Susumu Eguchi","doi":"10.1016/j.reth.2025.09.013","DOIUrl":"10.1016/j.reth.2025.09.013","url":null,"abstract":"","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Page 910"},"PeriodicalIF":3.5,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone morphogenetic protein-2-derived osteogenic peptide promotes bone regeneration via osteoblastogenesis","authors":"Jong-Bin Lee ,&nbsp;Ji-Youn Hong ,&nbsp;Hyeeun Shim ,&nbsp;Sehee Kim ,&nbsp;Dong Won Lee ,&nbsp;Bosun Kwon ,&nbsp;Jeong-Ho Yun","doi":"10.1016/j.reth.2025.09.006","DOIUrl":"10.1016/j.reth.2025.09.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Bone morphogenetic protein (BMP)-2 plays a critical role in stimulating human mesenchymal stromal cells (hMSCs) differentiation, a key process in bone regeneration. However, the clinical application of BMP-2 has been hindered by several adverse effects. This study evaluated the effectiveness of a newly synthesized BMP2-derived osteogenic peptide (OP), which may overcome the limitations of BMP-2 while preserving its osteogenic potential.</div></div><div><h3>Methods</h3><div>OP5, selected from the OP family based on its osteogenic potential, was tested <em>in vitro</em> to compare its effects on osteogenic signaling, osteoblast differentiation, and hMSC gene expression in with those of BMP-2. New bone formation stimulated by OP5 or BMP-2 was assessed <em>in vivo</em> using radiographic and histological analyses in a rat model of calvarial defects.</div></div><div><h3>Results</h3><div>The optimal OP5 concentration of 1 μM supported hMSC viability and exhibited potent osteogenic activity. OP5 significantly activated BMP receptor types IA and II binding and the osteogenic protein kinase A and phosphorylated cAMP response element-binding protein signaling pathway. OP5-induced gene expressions of alkaline phosphatase and osteocalcin peaked on day 4 (early osteogenesis) and were sustained until day 14 (late osteogenesis). <em>In vivo</em>, 100 μg OP5 demonstrated superior bone formation compared to other doses (50, 300, and 600 μg), but was less effective than BMP-2. The amount of bone regeneration varied with different doses of OP5.</div></div><div><h3>Conclusions</h3><div>OP5, a low-molecular-weight peptide with strong osteogenic potential, may be a viable alternative to BMP-2 for clinical bone regeneration, minimizing BMP2-associated adverse effects.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 911-919"},"PeriodicalIF":3.5,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of permeability in deep tissue capillaries using a new method reflects the nutrient supply status in a healthy heart","authors":"Mio Nakamura ,&nbsp;Yurika Yoshida-Kikkawa ,&nbsp;Kousuke Sugiura ,&nbsp;Yoko Itakura ,&nbsp;Kensuke Ohse ,&nbsp;Norihiko Sasaki ,&nbsp;Yuzuru Ito ,&nbsp;Masashi Toyoda","doi":"10.1016/j.reth.2025.10.005","DOIUrl":"10.1016/j.reth.2025.10.005","url":null,"abstract":"<div><h3>Introduction</h3><div>The <em>in vitro</em> organoid model is a valuable tool for studying organ development and disease. However, a key current challenge is the absence of a functional vascular compartment, which results from limited understanding of capillary function <em>in vivo</em>. Multisystem disorders involve physiological abnormalities that affect different organs in various ways. Notably, women have a higher prevalence of microvessel disease in the heart compared to men. This is because, until now, there has been no way to detect or evaluate the material exchange functions carried out by capillaries deep within healthy heart tissue.</div></div><div><h3>Methods</h3><div>To detect fluorescent material leaking from intracardiac microvessels deep within the left ventricular wall under healthy conditions, female mice were injected with fluorescent dextran via the tail vein. The heart tissue was quickly removed, frozen, sliced, and examined directly under fluorescence. Additionally, the extent of the fluorescent substance diffusion was measured in young and aged female mice.</div></div><div><h3>Results</h3><div>We observed fluorescent substances leaking from deep heart capillaries under healthy conditions. We then developed a method to measure capillary permeability by assessing the diffusion area. Furthermore, this method showed that the permeability of capillaries in the hearts of aged female mice was lower than that of young female mice.</div></div><div><h3>Conclusion</h3><div>We have developed a method to assess capillary permeability in deep tissue. This research will improve our understanding of how capillaries exchange substances and support tissue function. This new method will not only provide new insights into studies of cardiac disease risk and sex differences, but also assist in developing more advanced <em>in vitro</em> models. It will further aid in refining the best cell transplantation techniques.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 900-909"},"PeriodicalIF":3.5,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalization of 3D printed PLGA-based scaffolds for bone regeneration","authors":"Xuan Yan ,&nbsp;Yanhua Wei ,&nbsp;Yicai Luo ,&nbsp;Ziwei Wu ,&nbsp;Zhe Liu ,&nbsp;Hongbing Liao","doi":"10.1016/j.reth.2025.10.003","DOIUrl":"10.1016/j.reth.2025.10.003","url":null,"abstract":"<div><div>Bone defect remains an intractable issue for clinical orthopedics owing to their varied sizes and irregular shapes. Poly (lactic acid-co-glycolic acid) (PLGA)-based artificial bone grafts has garnered considerable attention in bone repair owing to their outstanding biocompatibility and tunable biodegradability. 3D printing technology is a feasible means due to its ability to construct scaffolds with defined shapes for restoring bone defects in clinical practice. In this review, the physicochemical properties of PLGA and 3D printing technology are briefly introduced. In addition, diverse strategies to improve the osteogenic performance of 3D printed PLGA scaffolds are elaborated. Finally, current challenges and future perspectives of 3D printed PLGA scaffolds applied in clinical practice are proposed.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 884-899"},"PeriodicalIF":3.5,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primed human pluripotent stem cell-derived blastocyst-like cell aggregates with partial lineage specification","authors":"Kaori Mutsuda-Zapater ,&nbsp;Xiaopeng Wen ,&nbsp;Satoshi Imamura ,&nbsp;Shiho Terada ,&nbsp;Kyoko Sawada ,&nbsp;Kei Saito ,&nbsp;Ken-ichiro Kamei","doi":"10.1016/j.reth.2025.10.007","DOIUrl":"10.1016/j.reth.2025.10.007","url":null,"abstract":"<div><h3>Introduction</h3><div>Human blastoids, blastocyst-like structures assembled <em>in vitro</em>, hold great promise for decoding the earliest steps of human development. However, most existing protocols rely on naïve human pluripotent stem cells (hPSCs), which are chromosomally unstable and technically demanding.</div></div><div><h3>Methods</h3><div>We developed a method to generate human blastocyst-like cell aggregates directly from primed hPSCs using a thermoresponsive hydrogel. Generated blastocyst-like cell aggregates were evaluated for morphological features, lineage marker expression via immunocytochemistry, transcriptional profiles using single-cell RNA sequencing, and functional capacity through <em>in vitro</em> implantation assays.</div></div><div><h3>Results</h3><div>Primed hPSC-derived blastocyst-like cell aggregates recapitulated key morphological features of human blastocysts, including cyst formation and spatial expression of epiblast, trophectoderm, and primitive endoderm markers. Single-cell RNA sequencing revealed that a subset of cells showed transcriptional profiles resembling epiblast-, trophectoderm-, and primitive endoderm-like cells although a substantial proportion remained undifferentiated. Functionally, blastocyst-like cell aggregates demonstrated <em>in vitro</em> implantation potential, trophoblast differentiation, and secretion of human chorionic gonadotropin.</div></div><div><h3>Conclusions</h3><div>This work introduces a more accessible platform for generating human blastocyst-like cell aggregates from primed hPSCs, broadening their utility for investigating early development events. Insights gained from blastocyst-like cell aggregates have the potential to advance the modelling of early-onset diseases, drive innovations in regenerative therapies, and contribute to the development of assisted reproductive technologies.</div></div>","PeriodicalId":20895,"journal":{"name":"Regenerative Therapy","volume":"30 ","pages":"Pages 875-883"},"PeriodicalIF":3.5,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}