Pub Date : 2021-09-05DOI: 10.21608/nvvj.2021.203361
J. Fregeneda-Grandes, M. El sayed, H. Sayed
Antibiotic resistance among fish pathogens has become a major concern. Integrons play an important role in dissemination of antibiotics resistance genes among the different species of bacteria. This study was designed to determine the antibiotic susceptibility of 20 bacterial isolates were isolated from the skin of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) collected from the rivers and fish farms in León Province, Spain and to detect class 1 and 2 integrons in these isolates by PCR to explore the role of these integrons in the antibiotic resistance. The investigated isolates included Pseudomonas fluorescens (P. fluorescens) (n=5), Aeromonas sobria (A. sobria) (n=3), Pantoea agglomerans (P. agglomerans) (n=3), Acinetobacter johnsonii (A. johnsonii) (n=2), Serratia fonticola (S. fonticola) (n=1), Staphylococcus equorum (S. equorum) (n=3), Yersinia kristensenii (Y. kristensenii) (n=2) and Xanthomonas retroflexus (X. retroflexus) (n=1). Antimicrobial susceptibility testing for the isolates revealed that they were sensitive to enrofloxacin (100%) and gentamicin (95%) while resistant to ampicillin (90%) and tetracycline (80%). PCR revealed that all the isolates didn’t harbor class 1 or 2 integrons. Therefore, the indiscriminate use of antibiotics must be restricted and more attention should be paid to biosecurity measures in aquaculture. Also, further studies are needed to assess role of integrons and other genetic elements in antimicrobial resistance in aquaculture.
{"title":"Antibiotic resistance in bacteria isolated from the skin of trout in León Province, Spain","authors":"J. Fregeneda-Grandes, M. El sayed, H. Sayed","doi":"10.21608/nvvj.2021.203361","DOIUrl":"https://doi.org/10.21608/nvvj.2021.203361","url":null,"abstract":"Antibiotic resistance among fish pathogens has become a major concern. Integrons play an important role in dissemination of antibiotics resistance genes among the different species of bacteria. This study was designed to determine the antibiotic susceptibility of 20 bacterial isolates were isolated from the skin of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) collected from the rivers and fish farms in León Province, Spain and to detect class 1 and 2 integrons in these isolates by PCR to explore the role of these integrons in the antibiotic resistance. The investigated isolates included Pseudomonas fluorescens (P. fluorescens) (n=5), Aeromonas sobria (A. sobria) (n=3), Pantoea agglomerans (P. agglomerans) (n=3), Acinetobacter johnsonii (A. johnsonii) (n=2), Serratia fonticola (S. fonticola) (n=1), Staphylococcus equorum (S. equorum) (n=3), Yersinia kristensenii (Y. kristensenii) (n=2) and Xanthomonas retroflexus (X. retroflexus) (n=1). Antimicrobial susceptibility testing for the isolates revealed that they were sensitive to enrofloxacin (100%) and gentamicin (95%) while resistant to ampicillin (90%) and tetracycline (80%). PCR revealed that all the isolates didn’t harbor class 1 or 2 integrons. Therefore, the indiscriminate use of antibiotics must be restricted and more attention should be paid to biosecurity measures in aquaculture. Also, further studies are needed to assess role of integrons and other genetic elements in antimicrobial resistance in aquaculture.","PeriodicalId":210463,"journal":{"name":"New Valley Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130476667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-01DOI: 10.21608/nvvj.2021.91134.1000
samaa moataz, N. Saad, T. El-Bassiony
The objective of this study was to determine the presence of coliforms, fecal coliform and Escherichia coli in both baladi and farm table eggs in both shells and contents. A total of 100 farms and baladi eggs samples (50 samples of each) were randomly collected from poultry farms, markets, supermarkets, and groceries in Assiut governorate. Eggs were microbiologically examined, and isolates were identified by biochemical and (PCR). The obtained results revealed that coliform and fecal coliform incidences were 62, 56, 54 and 32% for the farm hens' eggshell, egg content, baladi hens' eggshell and egg content, respectively. The biochemical identification of revealed 16 isolates of E. coli and these results were complementary with the molecular identification. E.coli incidence was 6, 8, 12 and 6% for the farm hens' eggshell, egg content, baladi hens' eggshell and egg content, respectively. The public health significance and hygienic control measures were discussed in this study..
{"title":"Detection of Coliforms in table eggs","authors":"samaa moataz, N. Saad, T. El-Bassiony","doi":"10.21608/nvvj.2021.91134.1000","DOIUrl":"https://doi.org/10.21608/nvvj.2021.91134.1000","url":null,"abstract":"The objective of this study was to determine the presence of coliforms, fecal coliform and Escherichia coli in both baladi and farm table eggs in both shells and contents. A total of 100 farms and baladi eggs samples (50 samples of each) were randomly collected from poultry farms, markets, supermarkets, and groceries in Assiut governorate. Eggs were microbiologically examined, and isolates were identified by biochemical and (PCR). The obtained results revealed that coliform and fecal coliform incidences were 62, 56, 54 and 32% for the farm hens' eggshell, egg content, baladi hens' eggshell and egg content, respectively. The biochemical identification of revealed 16 isolates of E. coli and these results were complementary with the molecular identification. E.coli incidence was 6, 8, 12 and 6% for the farm hens' eggshell, egg content, baladi hens' eggshell and egg content, respectively. The public health significance and hygienic control measures were discussed in this study..","PeriodicalId":210463,"journal":{"name":"New Valley Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115554066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-01DOI: 10.21608/nvvj.2021.180749
M. A. Hassan, Marwa A Elzeftawy, A. Nany, Nasreldein, Fatma Abo Zakaib Ahmed Ali, T. Mahmoud, Nassef, M. El-Sherif
1 Department of Surgery, Faculty of Veterinary Medicine, New Valley University. 2 Department of Biochemistry, Faculty of Veterinary Medicine, New Valley University. 3 Department of Clinical Pathology, Faculty of Veterinary Medicine, New Valley University. 4 Department of Pathology, Faculty of Veterinary Medicine, Sohag University. 5 Department of Surgery, Faculty of Veterinary Medicine, Assiut University. Abstract
{"title":"The influence of autologous platelet rich plasma on healing of induced alkali burn corneal ulcer in rabbit model.","authors":"M. A. Hassan, Marwa A Elzeftawy, A. Nany, Nasreldein, Fatma Abo Zakaib Ahmed Ali, T. Mahmoud, Nassef, M. El-Sherif","doi":"10.21608/nvvj.2021.180749","DOIUrl":"https://doi.org/10.21608/nvvj.2021.180749","url":null,"abstract":"1 Department of Surgery, Faculty of Veterinary Medicine, New Valley University. 2 Department of Biochemistry, Faculty of Veterinary Medicine, New Valley University. 3 Department of Clinical Pathology, Faculty of Veterinary Medicine, New Valley University. 4 Department of Pathology, Faculty of Veterinary Medicine, Sohag University. 5 Department of Surgery, Faculty of Veterinary Medicine, Assiut University. Abstract","PeriodicalId":210463,"journal":{"name":"New Valley Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132009585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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