Research in microbiology最新文献

英文中文

Transposition of transposable element IS1 in Edwardsiella piscicida mutant generated by CRISPR/Cas9 along with λ-Red recombineering system CRISPR/Cas9和λ-Red重组系统在鱼腥爱德华菌突变体中的转座元件IS1的转座

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.resmic.2025.104297

Eun Gyeong Lee, Ki Hong Kim

This study aimed to investigate unintended mutations introduced by the CRISPR/Cas9 genome editing system in Edwardsiella piscicida. Whole-genome sequencing was conducted on the wild-type E. piscicida NH1 and its alanine racemase knockout mutants (E. piscicida Δalr325 NH1 and E. piscicida Δalr50 NH1) generated using CRISPR/Cas9 with a λ-Red recombineering system. Comparative genomic analyses revealed that the insertion sequence 1 (IS1) transpositions occurred in the CRISPR/Cas9-edited mutants, disrupting the type I restriction-modification system subunit M gene, in addition to the targeted gene deletion. Interestingly, no IS1 transpositions were detected in mutants produced via conventional plasmid-based allelic exchange, indicating the potential link between CRISPR/Cas9-mediated editing and transposition events. These results suggest that genome editing via CRISPR/Cas9 could trigger IS1 transposition, potentially due to double-stranded DNA breaks. The lack of sequence similarity between the single guide RNA (sgRNA) and the transposed regions suggests that transpositions are not CRISPR/Cas9 off-target effects. This study provides evidence of interactions between mobile genetic elements and genome editing systems, requiring further investigation into their underlying mechanisms.

本研究旨在研究CRISPR/Cas9基因组编辑系统在鱼畔爱德华菌中引入的意外突变。利用CRISPR/Cas9和λ-Red重组系统，对野生型piscicida NH1及其丙氨酸消旋酶敲除突变体piscicida Δalr325 NH1和piscicida Δalr50 NH1进行全基因组测序。比较基因组分析显示，在CRISPR/ cas9编辑的突变体中发生了插入序列1 （IS1）转位，除了靶基因缺失外，还破坏了I型限制性修饰系统M亚基基因。有趣的是，在通过传统的基于质粒的等位基因交换产生的突变体中未检测到IS1转座，这表明CRISPR/ cas9介导的编辑与转座事件之间存在潜在联系。这些结果表明，通过CRISPR/Cas9进行基因组编辑可能引发IS1转位，可能是由于双链DNA断裂。单导RNA （sgRNA）与转座区域之间缺乏序列相似性，表明转座不是CRISPR/Cas9脱靶效应。这项研究提供了移动遗传元件和基因组编辑系统之间相互作用的证据，需要进一步研究其潜在机制。

{"title":"Transposition of transposable element IS1 in Edwardsiella piscicida mutant generated by CRISPR/Cas9 along with λ-Red recombineering system","authors":"Eun Gyeong Lee, Ki Hong Kim","doi":"10.1016/j.resmic.2025.104297","DOIUrl":"10.1016/j.resmic.2025.104297","url":null,"abstract":"<div><div>This study aimed to investigate unintended mutations introduced by the CRISPR/Cas9 genome editing system in <em>Edwardsiella piscicida</em>. Whole-genome sequencing was conducted on the wild-type <em>E. piscicida NH1</em> and its alanine racemase knockout mutants (<em>E. piscicida Δalr325 NH1</em> and <em>E. piscicida Δalr50 NH1</em>) generated using CRISPR/Cas9 with a λ-Red recombineering system. Comparative genomic analyses revealed that the insertion sequence 1 (IS1) transpositions occurred in the CRISPR/Cas9-edited mutants, disrupting the type I restriction-modification system subunit M gene, in addition to the targeted gene deletion. Interestingly, no IS1 transpositions were detected in mutants produced via conventional plasmid-based allelic exchange, indicating the potential link between CRISPR/Cas9-mediated editing and transposition events. These results suggest that genome editing via CRISPR/Cas9 could trigger IS1 transposition, potentially due to double-stranded DNA breaks. The lack of sequence similarity between the single guide RNA (sgRNA) and the transposed regions suggests that transpositions are not CRISPR/Cas9 off-target effects. This study provides evidence of interactions between mobile genetic elements and genome editing systems, requiring further investigation into their underlying mechanisms.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 5","pages":"Article 104297"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In memoriam Julian Edmund Davies (1932–2025) 纪念朱利安·埃德蒙·戴维斯（1932-2025）。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-07-01 DOI: 10.1016/j.resmic.2025.104302

Tarek Msadek

引用次数: 0

Highly specific virus-host linkages in mesothermophilic and circumneutral hot springs microbial communities. 中温和环中性温泉微生物群落的高度特异性病毒-宿主联系。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-06-13 DOI: 10.1016/j.resmic.2025.104300

Oscar Salgado, Oscar Martínez, Yessica Rivas, Felipe Loyola-Ahumada

Microbial communities in hot springs are distributed globally and have been extensively characterized regarding their diversity and composition. However, most studies have focused on cellular microbes, with relatively few addressing viruses, and even fewer examining virus-host ecology. Furthermore, research on viral communities and virus-host interactions has predominantly targeted extremely thermophilic environments, leaving mesothermophilic (40-80 °C) and circumneutral (pH 6-8) hot spring less explored. Here, we describe virus-host linkages using 37 metagenomic datasets obtained from 14 mesothermophilic and circumneutral hot springs. A parallel analysis of hot spring habitats-sediment, microbial mat, and water-employing diversity metrics, network analyses, and machine learning for virus-host pair identification revealed that viral communities appear to be more endemic than their hosts. Additionally, certain host taxa were identified as differentially abundant across habitats, some corresponding to specific hubs in the network analyses. Most viruses were linked to hosts within the same metagenome, with only a small fraction classified as cosmopolitan. Furthermore, nearly all virus-host pairs were exclusive to a single habitat type-sediment, microbial mat, or water. These findings suggests that virus-host linkages in mesothermophilic and circumneutral hot springs are highly specific, reflecting ecological adaptation within habitat niches.

温泉微生物群落分布全球，其多样性和组成已被广泛研究。然而，大多数研究都集中在细胞微生物上，相对较少涉及病毒，更少研究病毒宿主生态学。此外，对病毒群落和病毒-宿主相互作用的研究主要针对极端嗜热环境，而对中嗜热（40-80°C）和环中性（pH 6-8）温泉的研究较少。在这里，我们使用来自14个中温和环中性温泉的37个宏基因组数据集描述了病毒与宿主的联系。对温泉栖息地（沉积物、微生物垫和水）的平行分析（采用多样性指标、网络分析和机器学习进行病毒-宿主对识别）显示，病毒群落似乎比它们的宿主更具地方性。此外，在网络分析中，某些寄主类群在不同生境中具有差异丰度，其中一些对应于特定的枢纽。大多数病毒与同一宏基因组内的宿主相关联，只有一小部分被归类为世界性病毒。此外，几乎所有的病毒-宿主对都只存在于一种栖息地类型——沉积物、微生物垫或水。这些发现表明，中温和环中性温泉中的病毒-宿主联系是高度特异性的，反映了生境生态位内的生态适应性。

{"title":"Highly specific virus-host linkages in mesothermophilic and circumneutral hot springs microbial communities.","authors":"Oscar Salgado, Oscar Martínez, Yessica Rivas, Felipe Loyola-Ahumada","doi":"10.1016/j.resmic.2025.104300","DOIUrl":"10.1016/j.resmic.2025.104300","url":null,"abstract":"<p><p>Microbial communities in hot springs are distributed globally and have been extensively characterized regarding their diversity and composition. However, most studies have focused on cellular microbes, with relatively few addressing viruses, and even fewer examining virus-host ecology. Furthermore, research on viral communities and virus-host interactions has predominantly targeted extremely thermophilic environments, leaving mesothermophilic (40-80 °C) and circumneutral (pH 6-8) hot spring less explored. Here, we describe virus-host linkages using 37 metagenomic datasets obtained from 14 mesothermophilic and circumneutral hot springs. A parallel analysis of hot spring habitats-sediment, microbial mat, and water-employing diversity metrics, network analyses, and machine learning for virus-host pair identification revealed that viral communities appear to be more endemic than their hosts. Additionally, certain host taxa were identified as differentially abundant across habitats, some corresponding to specific hubs in the network analyses. Most viruses were linked to hosts within the same metagenome, with only a small fraction classified as cosmopolitan. Furthermore, nearly all virus-host pairs were exclusive to a single habitat type-sediment, microbial mat, or water. These findings suggests that virus-host linkages in mesothermophilic and circumneutral hot springs are highly specific, reflecting ecological adaptation within habitat niches.</p>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":" ","pages":"104300"},"PeriodicalIF":2.5,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin 氨苄西林亚抑制浓度加重多重耐药大肠杆菌O104:H4的毒力。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2025.104266

Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García

Little is known about how subinhibitory concentrations of antibiotics to which bacteria are resistant affect bacterial virulence. In this study, the effect of subinhibitory concentrations of ampicillin on the virulence of E. coli O104:H4 was analyzed. Bacteria were pre-exposed to 0.1, 0.3, or 0.5 mg/mL of ampicillin in LB media and incubated for 4 h at 37 °C. Transformation capacity (using plasmids and PCR-amplified DNA sequences), swarming motility, biofilm production, curli formation, and virulence gene expression were determined. Ampicillin increased the transformation of E. coli O104:H4, with the highest number of transformants (>10⁴ CFU/ng DNA; p ≤ 0.05) detected after exposure to DNA sequences of spectinomycin. In addition, bacteria pre-treated with 0.5 mg/mL of ampicillin exhibited higher swarming motility (7.6 cm, vs 6.0 cm for control; p ≤ 0.05) and biofilm production (up to 1.9-fold; p ≤ 0.05) when subsequently exposed to 0.1 and 0.3 mg/mL of antibiotic compared with the control. Also, significant overexpression of the virulence-related genes flhC (≤16.1-fold), fliA (≤22.1-fold), csgA (≤3.6-fold), csgD (≤9.1-fold), stx2a (≤32.2-fold), and the antibiotic resistance gene blaTEM-1 (≤5.5-fold) was observed. In conclusion, ampicillin-resistant E. coli O104:H4 increased the expression of its virulence factors when exposed to most subinhibitory concentrations of ampicillin analyzed in this study.

对于细菌耐药的抗生素的亚抑制浓度如何影响细菌的毒力，人们知之甚少。本研究分析了氨苄西林亚抑制浓度对大肠杆菌O104:H4毒力的影响。细菌在LB培养基中预先暴露于0.1、0.3或0.5 mg/mL氨苄西林中，在37℃下孵育4小时。检测转化能力（利用质粒和pcr扩增的DNA序列）、蜂群运动、生物膜生成、卷曲形成和毒力基因表达。氨苄西林增加了大肠杆菌O104:H4的转化，转化体数量最高(>104 CFU/ng DNA；p≤0.05)。此外，用0.5 mg/mL氨苄西林预处理的细菌表现出更高的群体能动性(7.6 cm，对照组为6.0 cm；P≤0.05)和生物膜产量(可达1.9倍；p≤0.05)，与对照组相比，随后暴露于0.1和0.3 mg/mL抗生素。毒力相关基因flhC（≤16.1倍）、fliA（≤22.1倍）、csgA（≤3.6倍）、csgD（≤9.1倍）、stx2a（≤32.2倍）和抗生素耐药基因blatem1（≤5.5倍）均显著过表达。综上所述，耐氨苄西林大肠杆菌O104:H4在暴露于本研究分析的大多数亚抑制浓度氨苄西林时，其毒力因子的表达增加。

{"title":"Exacerbation of virulence of multi-drug resistant Escherichia coli O104:H4 by subinhibitory concentrations of ampicillin","authors":"Yaraymi Ortiz, Vianey Lechuga, Carolina Ortiz, Eduardo Palomino, Eduardo Franco, Norma Heredia, Santos García","doi":"10.1016/j.resmic.2025.104266","DOIUrl":"10.1016/j.resmic.2025.104266","url":null,"abstract":"<div><div>Little is known about how subinhibitory concentrations of antibiotics to which bacteria are resistant affect bacterial virulence. In this study, the effect of subinhibitory concentrations of ampicillin on the virulence of <em>E. coli</em> O104:H4 was analyzed. Bacteria were pre-exposed to 0.1, 0.3, or 0.5 mg/mL of ampicillin in LB media and incubated for 4 h at 37 °C. Transformation capacity (using plasmids and PCR-amplified DNA sequences), swarming motility, biofilm production, curli formation, and virulence gene expression were determined. Ampicillin increased the transformation of <em>E. coli</em> O104:H4, with the highest number of transformants (>10<sup>4</sup> CFU/ng DNA; <em>p</em> ≤ 0.05) detected after exposure to DNA sequences of spectinomycin. In addition, bacteria pre-treated with 0.5 mg/mL of ampicillin exhibited higher swarming motility (7.6 cm, vs 6.0 cm for control; <em>p</em> ≤ 0.05) and biofilm production (up to 1.9-fold; <em>p</em> ≤ 0.05) when subsequently exposed to 0.1 and 0.3 mg/mL of antibiotic compared with the control. Also, significant overexpression of the virulence-related genes <em>flhC</em> (≤16.1-fold), <em>fliA</em> (≤22.1-fold), <em>csgA</em> (≤3.6-fold), <em>csgD</em> (≤9.1-fold), <em>stx2a</em> (≤32.2-fold), and the antibiotic resistance gene <em>blaTEM-1</em> (≤5.5-fold) was observed. In conclusion, ampicillin-resistant <em>E. coli</em> O104:H4 increased the expression of its virulence factors when exposed to most subinhibitory concentrations of ampicillin analyzed in this study.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 3","pages":"Article 104266"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutibacterium acnes and its complex host interaction in prosthetic joint infection: Current insights and future directions 痤疮表皮杆菌及其复杂的宿主相互作用在假体关节感染：目前的见解和未来的方向。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104265

Léa Thoraval , Jennifer Varin-Simon , Xavier Ohl , Frédéric Velard , Fany Reffuveille , Min Tang-Fichaux

Cutibacterium acnes is a commensal Gram-positive anaerobic bacterium that can also act as an opportunistic pathogen in various diseases, particularly in prosthetic joint infections (PJI). Throughout this review, we delve into the current understanding of the intricate interactions between C. acnes and host cells and discuss bacterial persistence in the host. C. acnes colonization and subsequent PJI set-up represent complex processes involving bacterial adhesion, immune recognition, and host response mechanisms. We highlight existing knowledge and gaps in specific host-pathogen interactions and stress the importance of acquiring additional information to develop targeted strategies for preventing and treating C. acnes-related PIJ.

痤疮表皮杆菌是一种共生革兰氏阳性厌氧菌，在各种疾病中也可以作为机会性病原体，特别是在假体关节感染（PJI）中。在这篇综述中，我们深入研究了目前对痤疮芽孢杆菌与宿主细胞之间复杂相互作用的理解，并讨论了细菌在宿主中的持久性。C.痤疮定植和随后的PJI建立代表了涉及细菌粘附、免疫识别和宿主反应机制的复杂过程。我们强调了在特定宿主-病原体相互作用方面的现有知识和空白，并强调了获取额外信息以制定预防和治疗痤疮C.相关PIJ的有针对性策略的重要性。

引用次数: 0

Staphylococcus aureus: Dynamics of pathogenicity and antimicrobial-resistance in hospital and community environments - Comprehensive overview 金黄色葡萄球菌：医院和社区环境中致病性和抗菌药耐药性的动态 - 综合概述。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2025.104267

Giorgio Silva-Santana

This study reviews Staphylococcus aureus, a significant pathogen in both hospital and community-acquired infections, addressing its epidemiology, pathogenesis, and antimicrobial resistance. It highlights virulence mechanisms, such as adhesion factors, toxins, enzymes, and biofilms, which contribute to survival and immune evasion. The spread of resistance occurs through the transfer of mobile genetic elements like SCCmec and genetic mutations. The analysis also compares hospital and community strains, including multidrug-resistant lineages like MRSA, VISA, and VRSA. The study concludes that S. aureus presents a major public health challenge, requiring new therapeutic approaches and preventive strategies.

本研究综述了金黄色葡萄球菌这一在医院和社区获得性感染中的重要病原体，阐述了其流行病学、发病机制和抗微生物药物耐药性。它强调了毒力机制，如粘附因子、毒素、酶和生物膜，它们有助于生存和免疫逃避。抗性的传播是通过SCCmec等可移动遗传元素的转移和基因突变发生的。该分析还比较了医院和社区菌株，包括耐多药谱系，如MRSA、VISA和VRSA。该研究得出结论，金黄色葡萄球菌提出了一个重大的公共卫生挑战，需要新的治疗方法和预防策略。

引用次数: 0

Actinomycetes studies in Tunisia 突尼斯放线菌研究。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2025.104279

Souleima Dhahbi, Jeonghee Lee, Dohee Ryu, Ganiyu Akinniyi, Inho Yang

Tunisia, located in North Africa, has a diverse topography along the Mediterranean Sea to the Sahara Desert. These environments encompass oases, rhizosphere soils, desert deposits, saline wetlands, offshore oilrigs, and ancient monument rocks. The country's varied environments have led to the isolation of a multitude of actinomycetes. A phylogenetic analysis based on the 16S rRNA sequences of one hundred isolated actinomycetes strains revealed that the majority belong to the genus Streptomyces. Secondary metabolite studies from these actinomycetes yielded 33 natural products. Notably, compound 12, 3-O-methylviridicatin, exhibited antitumor activity and suppressed HIV expression. This showcases Tunisia's potential for natural product research.

突尼斯位于北非，从地中海到撒哈拉沙漠，地形多样。这些环境包括绿洲、根际土壤、沙漠沉积物、含盐湿地、海上石油钻井平台和古代纪念碑岩石。该国多变的环境导致了大量放线菌的分离。通过对100株分离的放线菌的16S rRNA序列进行系统发育分析，发现大多数放线菌属于链霉菌属。这些放线菌的次生代谢物研究产生了33种天然产物。值得注意的是，化合物12,3 - o -甲基vi可笑蛋白具有抗肿瘤活性并抑制HIV的表达。这显示了突尼斯在天然产品研究方面的潜力。

引用次数: 0

Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere 大豆（Glycine max L.）根际抗砷促进植物生长细菌Pseudomonas sp. AW4的鉴定与鉴定

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104263

Ana L. Wevar Oller , Gonzalo Torres Tejerizo , Paola P. Pereira , Romina del Pilar Pramparo , Elizabeth Agostini

Pseudomonas sp. AW4 is a highly arsenic (As) resistant bacterium with plant growth promoting properties, originally isolated from the soybean (Glycine max L.) rhizosphere. In order to safely use this isolate in diverse bioformulations, its characterization needs to be completed and a reliable identification must be provided. In the present work, we analyzed the morpho-physiological, biochemical and genomic characteristics of Pseudomonas sp. AW4. Identification of the isolate varied according to the parameters analyzed, mainly biochemical and physiological tests or individual genes and phylogenetic analyses. In this regard, we performed massive sequencing of its genome, in order to consistently complete its characterization and identification. Pseudomonas sp. AW4 formed a monophyletic clade with P. urmiensis SWRI10, presenting 3.08 % of unique genes against this reference isolate. More than 70 % of AW4 genes were also shared with P. oryziphila strain 1257 NZ and with P. reidholzensis strain CCOS 865. The search for genes related to As resistance evidenced the presence of the operon arsHRBC. Taken together, results of the present work allow identification of this bacterium as Pseudomonas urmiensis AW4 and open up a number of opportunities to study this strain and understand the mechanisms of arsenic resistance and plant growth promotion.

Pseudomonas sp. AW4是一种具有促进植物生长特性的高砷抗性细菌，最初从大豆（Glycine max L.）根际分离得到。为了在不同的生物制剂中安全地使用该分离物，需要完成其表征并提供可靠的鉴定。本文对假单胞菌AW4的形态、生理、生化和基因组特征进行了分析。分离物的鉴定根据分析的参数不同而不同，主要是生化和生理试验或个体基因和系统发育分析。为此，我们对其进行了大量的基因组测序，以一致地完成其表征和鉴定。假单胞菌sp. AW4与P. urmiensis SWRI10形成了一个单系分支，与该参考分离物具有3.08%的独特基因。oryziphila菌株1257 NZ和P. reidholzensis菌株CCOS 865共有70%以上的AW4基因。对As抗性相关基因的研究证实了操纵子arsHRBC的存在。综上所述，本研究结果为鉴定该细菌为乌尔米假单胞菌AW4提供了机会，并为研究该菌株以及了解其抗砷和促进植物生长的机制开辟了许多机会。

{"title":"Characterization and identification of Pseudomonas sp. AW4, an arsenic-resistant and plant growth-promoting bacteria isolated from the soybean (Glycine max L.) rhizosphere","authors":"Ana L. Wevar Oller , Gonzalo Torres Tejerizo , Paola P. Pereira , Romina del Pilar Pramparo , Elizabeth Agostini","doi":"10.1016/j.resmic.2024.104263","DOIUrl":"10.1016/j.resmic.2024.104263","url":null,"abstract":"<div><div><em>Pseudomonas</em> sp. AW4 is a highly arsenic (As) resistant bacterium with plant growth promoting properties, originally isolated from the soybean (<em>Glycine</em> max L.) rhizosphere. In order to safely use this isolate in diverse bioformulations, its characterization needs to be completed and a reliable identification must be provided. In the present work, we analyzed the morpho-physiological, biochemical and genomic characteristics of <em>Pseudomonas</em> sp. AW4. Identification of the isolate varied according to the parameters analyzed, mainly biochemical and physiological tests or individual genes and phylogenetic analyses. In this regard, we performed massive sequencing of its genome, in order to consistently complete its characterization and identification. <em>Pseudomonas</em> sp. AW4 formed a monophyletic clade with <em>P. urmiensis</em> SWRI10, presenting 3.08 % of unique genes against this reference isolate. More than 70 % of AW4 genes were also shared with <em>P. oryziphila</em> strain 1257 NZ and with <em>P. reidholzensis</em> strain CCOS 865. The search for genes related to As resistance evidenced the presence of the operon <em>arsHRBC</em>. Taken together, results of the present work allow identification of this bacterium as <em>Pseudomonas urmiensis</em> AW4 and open up a number of opportunities to study this strain and understand the mechanisms of arsenic resistance and plant growth promotion.</div></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":"176 3","pages":"Article 104263"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights into Bacillus thuringiensis: Beyond its role as a bioinsecticide 苏云金芽孢杆菌的新见解：超越其作为生物杀虫剂的作用。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104264

Gholamreza Salehi Jouzani , Reza Sharafi , Leandris Argentel-Martínez , Ofelda Peñuelas-Rubio , Ceyda Ozkan , Bengisu Incegul , Rana Goksu , Zehra Hayta , Deniz Yilki , Beyza Yazici , Vildan Hancer , Estibaliz Sansinenea , Jae-Ho Shin , A. El-Shabasy , Ugur Azizoglu

This review explores the diverse applications of Bacillus thuringiensis (Bt) beyond its traditional role as a bioinsecticide. Bt produces a variety of compounds with distinct chemical structures and biological activities. These include antimicrobial agents effective against plant pathogens and bioactive compounds that promote plant growth through the production of siderophores, hormones, and enzymes. Additionally, Bt's industrial potential is highlighted, encompassing biofuel production, bioplastics, nanoparticle synthesis, food preservation, anticancer therapies, and heavy metal bioremediation. This critical analysis emphasizes recent advancements and applications, providing insights into Bt's role in sustainable agriculture, biotechnology, and environmental management.

本文综述了苏云金芽孢杆菌（Bacillus thuringiensis, Bt）在传统生物杀虫剂之外的多种应用。Bt产生多种具有不同化学结构和生物活性的化合物。这些包括对植物病原体有效的抗菌剂和通过产生铁载体、激素和酶来促进植物生长的生物活性化合物。此外，Bt的工业潜力也得到了强调，包括生物燃料生产、生物塑料、纳米颗粒合成、食品保鲜、抗癌治疗和重金属生物修复。这种批判性的分析强调了最近的进展和应用，提供了对Bt在可持续农业，生物技术和环境管理中的作用的见解。

引用次数: 0

Navigating dual-species fungal biofilms: The competitive and cooperative dynamics of Candida albicans 导航双种真菌生物膜：念珠菌的竞争与合作动态。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology

Pub Date : 2025-03-01 DOI: 10.1016/j.resmic.2024.104262

Ouassila Bekkal Brikci-Benhabib

Research on microbial biofilms has primarily concentrated on bacterial-bacterial and bacterial-fungal interactions, leaving fungal-fungal dynamics underexplored. The present study examines interactions within dual-species fungal biofilms, with a particular emphasis on Candida albicans. The behavior and pathogenicity of this yeast are significantly influenced by its interactions with other fungal species in biofilms, where its ability to shift between yeast and hyphal forms contributes significantly to biofilm formation. These fungal species biofilms exhibit a complex interplay of synergistic cooperation and antagonistic competition, depending on the environmental context and resource availability. Understanding these interactions is essential for advancing our knowledge of fungal biofilm.

对微生物生物膜的研究主要集中在细菌-细菌和细菌-真菌的相互作用上，而对真菌-真菌的动力学研究不足。本研究考察了双物种真菌生物膜内的相互作用，特别强调白色念珠菌。这种酵母菌的行为和致病性受其与生物膜中其他真菌物种的相互作用的显著影响，其在酵母和菌丝形式之间转换的能力对生物膜的形成有重要贡献。这些真菌物种的生物膜表现出协同合作和拮抗竞争的复杂相互作用，这取决于环境背景和资源的可用性。了解这些相互作用对于提高我们对真菌生物膜的认识至关重要。

引用次数: 0

首页上一页

下一页尾页

类型

全部化学•材料生命科学医学物理工程技术环境•农林材料科学地球科学法学管理学化学环境科学与生态学计算机科学教育学经济学农林科学人文科学生物学数学物理与天体物理心理学综合性期刊其他工业工程理学历史学农学文学信息工程

数据库

全部 ACS Publications Elsevier ieeexplore Springer The Royal Society of Chemistry Wiley

期刊

Research in microbiology

全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.

﹀