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Genetic contributions to productivity and nutritional aspects in cassava crops 遗传对木薯作物生产力和营养方面的影响
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.14090
A. Bester, I. Carvalho, Murilo Vieira Loro, A. H. Ramos, Inaê Carolina Sfalcin, J. G. D. Silva, Felipe da Rosa Foguesatto, Marcos Vinícius Uhde Foguesatto
This study aimed to highlight the behavior of cassava cultivars when subjected to different densities and biostimulants at planting and to select superior cultivars based on nutritional and productive attributes using the multivariate approach. The experiment design used randomized blocks in a three-factor scheme, with three cassava cultivars (FEPAGRO-RS 13 Vassourinha, BRS CS01, Iapar - 19 Pioneira) × two planting densities (10 and 20 buds per linear meter) × two biostimulator forms (with and without) in three replications, totaling 36 experiment units. Cultivar BRS CS01 had the highest yield and concentration of mineral material, genotype FEPAGRO - RS 13 Vassourinha had the highest lipid content, and Iapar 19 - Pioneira had the highest protein concentrations. The starch content was tested with a comparison of means and MGIDI index. Cultivar FEPAGRO - RS 13 Vassourinha had the highest content and, according to the index, was the ideal cultivar based on multi-characteristics. Density 10 with the biostimulator was favorable for productivity and lipids, whereas density 10 without the biostimulator was favorable for starch, lipids, proteins and productivity. Density 20 with the biostimulator was favorable for lipids.
本研究旨在揭示木薯品种在种植过程中在不同密度和生物刺激物作用下的行为,并利用多变量方法根据营养和生产属性选择优质品种。试验设计采用三因子随机分组设计,3个木薯品种(FEPAGRO-RS 13 Vassourinha, BRS CS01, Iapar - 19 pioneer) × 2种种植密度(每线性米10和20个芽)× 2种生物刺激形式(带和不带),3个重复,共36个试验单元。品种BRS CS01产量和矿物物质含量最高,基因型FEPAGRO - rs13 Vassourinha脂肪含量最高,基因型Iapar 19 - pioneer含量最高。采用均值与MGIDI指数对比法测定淀粉含量。品种FEPAGRO - rs13 Vassourinha含量最高,是综合多种性状的理想品种。有生物刺激剂的密度10有利于生产效率和脂质,而没有生物刺激剂的密度10有利于淀粉、脂质、蛋白质和生产效率。密度为20的生物刺激剂对脂质有利。
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引用次数: 0
CO2 capture and water use efficiency in Opuntia stricta (Haw.) at different seasons and evaluation times 不同季节和评估时间下,狭角麻的CO2捕集和水利用效率
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.13525
J. Souza, J. Araújo, E. D. S. Félix, R. D. C. Alves, Tarcísio José de Oliveira Filho, E. C. D. Lira
The forage cactus Mexican Elephant Ear is widely incorporated into the animal productive chain of the northeast region of Brazil. However, there is a lack of studies on the physiological dynamics of this cactus. Therefore, this study was conducted at the Estação Experimental Prof. Ignácio Salcedo of the Instituto Nacional do Semiárido (INSA), in Campina Grande, State of Paraíba, Brazil. The aim of this study was to evaluate CO2 uptake and water-use efficiency levels in Opuntia stricta (Haw.) during different seasons and evaluation times. The treatments were distributed using a 24×2 factorial arrangement, which corresponded to the evaluation of gas exchange processes for 24 hours in the rainy (June) and dry (December) seasons. The evaluated parameters were stomatal conductance, transpiration and CO2 uptake rate, internal CO2 concentration, instantaneous water-use efficiency and intrinsic water-use efficiency. The results revealed that gas exchange in the forage cactus was more intense during the rainy season, with good stability, than the low exchange levels during the dry season. Regardless of the season, the CO2 uptake peaked between 24:00 and 02:00. In addition, this range of time is the most suitable to conduct analyses under field conditions.
草料仙人掌墨西哥象耳被广泛纳入巴西东北地区的动物生产链。然而,对这种仙人掌的生理动力学研究缺乏。因此,这项研究是在巴西Paraíba州Campina Grande的国家研究所Semiárido (INSA)的esta 实验教授Ignácio Salcedo进行的。本研究的目的是评估不同季节和评估时间下,狭口麻(Opuntia stricta, Haw.)的CO2吸收和水分利用效率水平。处理采用24×2因子排列,对应于对多雨季节(6月)和干旱季节(12月)24小时气体交换过程的评价。评价参数为气孔导度、蒸腾和CO2吸收速率、内部CO2浓度、瞬时水分利用效率和内在水分利用效率。结果表明:牧草仙人掌的气体交换在雨季较为强烈,且稳定性较好,而在旱季则较低;无论什么季节,二氧化碳的吸收在24:00到02:00之间达到顶峰。此外,这个时间范围最适合在现场条件下进行分析。
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引用次数: 0
Nitrogen-fixing bacteria and nitrogen fertilization on economic feasibility of tomato 固氮菌与施氮对番茄经济可行性的影响
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.13623
Ana María Gómez-Duque, C. S. Morales-Londoño, Alejandro Hurtado-Salazar, Nelson Ceballos-Aguirre
To increase the availability of nutrients and improve crop productivity, chemical fertilizers are introduced to the soil, although the biological fixation of nitrogen contributes globally with 180 million metric tons of ammonia per year, which is comparable to current contribution of anthropogenic nitrogen. In this way, the present study aimed to evaluate the effect of the interaction of Azospyrillum brasilense and Bradyrhizobium japonicum with different levels of nitrogen on the yield and economic viability of the tomato crop. An experimental design of divided plots was used, with the nitrogen fertilization dose being the largest plot (100% of the dose, 50% of the dose and 0% of the dose) and the minor plot of nitrogen-fixing bacteria (100 cc ha-1, 200 cc ha-1, 300 cc ha-1) with four random internal blocks and five plants as experimental unit. The variables evaluated were: production per plant, yield/ha, number of fruits/plant and average fruit weight. Finally, the economic analysis was performed according to the combination of the treatments. The results obtained showed that the mixture of nitrogen-fixing bacteria (BFN) (Azospyrillum brasilense and Bradyrhizobium japonicum) at a dose of 100 cc ha-1, significantly increased tomato production when nitrogen (urea) was not applied. The application of BFN in combination with adequate levels of nitrogen favors the sustainability of the tomato.
为了增加养分的可用性和提高作物生产力,向土壤中施用了化肥,尽管全球每年生物固氮贡献1.8亿吨氨,这与目前人为氮的贡献相当。因此,本研究旨在评价不同施氮水平下巴西氮瘟菌和日本慢生根瘤菌互作对番茄产量和经济活力的影响。采用分块试验设计,施氮量为最大块(施氮量100%、施氮量50%、施氮量0%),固氮菌小块(施氮量100cc ha-1、200cc ha-1、300cc ha-1),随机设4个内块,5株植物为试验单元。评估的变量为:单株产量、单株产量、单株果数和平均果重。最后,对不同处理组合进行了经济分析。结果表明,在不施氮(尿素)的情况下,固氮菌(巴西氮胞菌和日本慢生根瘤菌)混合施用100 cc ha-1可显著提高番茄产量。施用BFN与适当的氮素水平相结合有利于番茄的可持续性。
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引用次数: 0
Liming applications and the SPAD chlorophyll index and stomatal conductance in cocoa exposed to cadmium in the soil 施用石灰及土壤中镉对可可SPAD叶绿素指数和气孔导度的影响
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.14530
Karen Victoria Suárez-Parra, Cristian Santiago Castañeda-Serrano, F. Forero-Ulloa, P. Almanza-Merchán, Pablo-Cesar Serrano-Agudelo
Cadmium is a heavy metal that affects cell structures, such as walls and membranes, especially in the photosynthetic apparatus (PSII), chlorophylls, chloroplasts and stomata, producing losses in production quantity and quality. In addition, it is harmful to the health of humans and animals. The objective was to analyze the behavior of the relative chlorophyll index (SPAD units) and stomatal conductance in clone CCN-51 cacao plants every 45 days (45, 90, 135 and 180 days) after liming application. Four doses of a dolomite + agricultural gypsum mixture were applied, increasing Ca+2 saturation in the soil to 7, 8 and 9 cmolc kg-1. The control treatment did not have applications. The results indicated a reduction in SPAD units in the plants without liming, with high cadmium levels in the soil (3.3 mg kg-1), and there were no statistical differences in the other treatments, possibly because of edaphic factors such as pH, organic matter content and Al+3. The best stomatal conductance was observed with 7 cmolc kg-1 in the foliar gas exchange. Supersaturated liming applications efficiently reduce the losses in quality and quantity caused by the accumulation of cadmium in cacao plants.
镉是一种影响细胞壁和细胞膜等细胞结构的重金属,尤其影响光合机构(PSII)、叶绿素、叶绿体和气孔,造成产量和质量损失。此外,它对人类和动物的健康有害。目的分析施用石灰后每45 d(45、90、135和180 d) CCN-51无性系植株相对叶绿素指数(SPAD单位)和气孔导度的变化。施用4次白云石+农业石膏混合物,使土壤Ca+2饱和度分别提高到7、8和9 cmolc kg-1。对照处理没有应用。结果表明,在土壤镉含量较高(3.3 mg kg-1)的情况下,未施用石灰的植物SPAD单位减少,而其他处理之间无统计学差异,可能与pH、有机质含量和Al+3等土壤因素有关。叶面气体交换速率为7 cmolc kg-1时气孔导度最佳。过饱和石灰的施用有效地减少了镉在可可植株中积累所造成的质量和数量损失。
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引用次数: 0
SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) 哥伦比亚辣椒种质资源的SSR-HRM分子特性研究。(茄科)
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.13363
José Enrique Lopéz-Candelo, R. Viáfara-Vega, Heiber Cárdenas-Henao
This study was the first evaluation of the genetic diversity of cultivated populations of habanero pepper (Capsicum chinense) in Colombia using SSR-HRM. Three habanero pepper lines were characterized with eight microsatellite markers using the High-Resolution Melt (HRM) technique. Twenty-seven individuals from the HL-original line and 30 individuals each from derived lines HL-70 and HL67 were genotyped. Three microsatellites were monomorphic, and five were polymorphic; however, a high allelic diversity was detected in the homozygous state in the 87 individuals. The Ng8 marker differentiated the HL-original and HL-67 lines from the HL-70 line with HRM profiles. The analysis of molecular variance (AMOVA) revelated that 52% of the genetic variation existed within lines. The HL-67 line was more similar to the HL-original line than to the HL-70 line. HL-70 recorded the highest genetic diversity for the derived lines and, therefore, could be used in a new breeding program. In contrast, the HL-67 line, because of its high genetic homogeneity, could potentially be used to evaluate different environmental conditions to find optimal conditions that increase productivity and pungency. Finally, comparing the HRM profiles with the monomorphic markers (Ng 33, Ng 18 and Ng 10) differentiated the C. chinense and C. frutescens species, which was difficult because of high morphologic similarity between these two species and is usually evaluated at the flowering stage, while HRM profiles can be done at any plant stage.
本研究首次利用SSR-HRM对哥伦比亚哈瓦那辣椒(Capsicum chinense)栽培群体的遗传多样性进行评价。利用高分辨率熔融(HRM)技术对3个哈瓦那辣椒品系进行了8个微卫星标记的鉴定。对hl -原系27个个体和衍生系HL-70和HL67各30个个体进行了基因分型。单态微卫星3颗,多态微卫星5颗;然而,在87个个体的纯合子状态下检测到较高的等位基因多样性。Ng8标记将HL-original和HL-67系与HL-70系进行了区分。分子方差分析(AMOVA)表明,52%的遗传变异存在于系内。HL-67系与hl原系的相似性大于HL-70系。HL-70的遗传多样性最高,因此可用于新的育种计划。相比之下,HL-67系由于其高遗传同质性,可以潜在地用于评估不同的环境条件,以找到提高生产力和辛辣度的最佳条件。最后,将HRM图谱与单态标记(Ng 33、Ng 18和Ng 10)进行比较,对C. chinense和C. frutescens进行区分,这是因为这两个物种之间的形态高度相似,并且通常在开花阶段进行评估,而HRM图谱可以在任何植物阶段进行评估。
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引用次数: 1
Broccoli and cauliflower seedling emergence and production in different substrates 西兰花和花椰菜在不同基质下的出苗和生产
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.14339
Tatiana Tasquetto Fiorin, J. F. Menegaes, Andrielle Magrini Rodrigues, A. Swarowsky
Inflorescence brassicas, such as broccoli and cauliflower, rich in fiber and vitamins, are widely consumed in Brazil, and present an average production of 23 t ha-1. Despite this demand, the quality of seedlings of both species is one of the main factors that limit production. Thus, the objective of this study was to evaluate broccoli and cauliflower seedling emergence and production in different substrates, using the irrigation method named Deep Film Technique (DFT) on a table. The experiment was carried out in a completely randomized design, 7×2: substrate compositions (volumetric proportions of 1:0:0; 0:1:0; 0:0:1; 1:1:1; 1:1:0; 1:0:1 and 0:1:1, containing commercial substrate Carolina Soil®, carbonized rice husk and medium textured sand) and inflorescence brassicas (broccoli and cauliflower), with four replications. Each experimental unit consisted of 50 seeds/seedlings. At 21 days after sowing (DAS), it was evaluated the percentage, index, time, speed and frequency of emergence, and, at 35 DAS, clod stability, number of leaves and seedling length. It was observed that there was good seed germination and seedling formation for both species in all substrate compositions and with the aid of the DFT irrigation method on a table. Under these conditions, for both species, substrate compositions of 1:0:0; 1:1:1 and 1:1:0 are recommended.
花椰菜和花椰菜等花椰菜富含纤维和维生素,在巴西被广泛食用,平均产量为23吨/公顷。尽管有这种需求,但两种树种的幼苗质量是限制产量的主要因素之一。因此,本研究的目的是评估西兰花和花椰菜在不同基质上的出苗和产量,采用灌溉方法称为深膜技术(DFT)在桌子上。实验采用完全随机设计,7×2:底物组成(体积比为1:0:0;0:1:0;0:0:1;比;1:1:0;1:0:1和0:1:1,含有商业基质Carolina Soil®,碳化稻壳和中等纹理砂)和花序芸苔(西兰花和花椰菜),四次重复。每个实验单元由50个种子/幼苗组成。在播后21天(DAS)评估出苗百分比、指数、时间、速度和频率,在播后35天(DAS)评估冷稳定性、叶片数和幼苗长。结果表明,两种植物在不同基质条件下均有良好的种子萌发和成苗效果。在此条件下,两种植物的基质组成为1:0:0;建议使用1:1:1和1:1:0。
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引用次数: 1
Effect of inoculation with Acaulospora and Glomus on the growth and nutrition of blueberries (Vaccinium corymbosum) with different fertilization levels 接种Acaulospora和Glomus对不同施肥水平蓝莓生长和营养的影响
Pub Date : 2022-05-01 DOI: 10.17584/rcch.2022v16i2.13561
G. Roveda-Hoyos, Julián Felipe Venegas-Gómez, L. Moreno-Fonseca, Stanislav Magnitskiy, Margarita Ramírez-Gómez
In recent years, the global demand for blueberries has been growing because of its nutraceutical properties in the fruits, which provide important benefits to human health. Colombia, thanks to its diversity, has a great opportunity to meet the blueberry demands of the global markets. In the present study, the effect of two arbuscular mycorrhizal fungi (AMF) of the genera Glomus and Acaulospora were evaluated on the growth of blueberry plants var. Biloxi, subjected to three levels of chemical fertilization (100, 50 and 0%), with the 100% level being optimal. The blueberry plants inoculated with Glomus under conditions of nutrient deficiency (50AMF1+) increased dry mass, height, number of basal branches, leaf area, root/shoot ratio, and chlorophyll concentration, which were significantly higher than in the plants without inoculation with nutrient deficiency (0AMF- and 50AMF-). The plants inoculated with Glomus achieved an increase in height, while the plants inoculated with Acaulospora increased the number of basal branches when grown under a nutrient deficiency (50AMF+1) in relation to the uninoculated controls (0AMF- and 50AMF-). The results suggested that the best association of blueberry occurs with Glomus, with increased plant growth and nutrition (N, P, K, Ca, Mg, and S).
近年来,全球对蓝莓的需求一直在增长,因为其水果中的营养成分对人体健康有重要的好处。由于其多样性,哥伦比亚有很大的机会满足全球市场对蓝莓的需求。本研究研究了两种丛枝菌根真菌(AMF) Glomus和Acaulospora对蓝莓品种Biloxi生长的影响,在3个施肥水平(100%、50%和0%)下,以100%为最佳施肥水平。营养缺乏(50AMF1+)条件下接种Glomus的蓝莓植株干质量、株高、基枝数、叶面积、根冠比和叶绿素浓度均显著高于营养缺乏(0AMF-和50AMF-)条件下未接种Glomus的蓝莓植株。与未接种对照(0AMF-和50AMF-)相比,在营养缺乏(50AMF+1)条件下,接种Glomus的植株高度增加,而接种Acaulospora的植株基部分枝数增加。结果表明,随着植株生长和营养(N、P、K、Ca、Mg和S)的增加,蓝莓与Glomus的关系最好。
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引用次数: 2
Estimating chlorophyll and nitrogen contents in maize leaves (Zea mays L.) with spectroscopic analysis 利用光谱分析估算玉米叶片叶绿素和氮含量
Pub Date : 2022-01-01 DOI: 10.17584/rcch.2022v16i1.13398
Carlos Arturo Ramos-García, Luis Joel Martínez-Martínez, J. Bernal-Riobo
The relationship between reflectance and chlorophyll (Chl) and nitrogen (N) contents in maize leaves was analyzed to identify useful spectral indices for diagnosing the nutritional status of crops in terms of N. An experiment was carried out in random blocks with five treatments of nitrogen fertilizer (0, 50, 100, 150, 200 kg ha-1) and four repetitions and the foliar spectral responses were measured with a FieldSpec 4 spectroradiometer in three phenological stages. Several spectral indices and values of red edge position (REP) were calculated using various methods. Red-edge position linear interpolation (REP-L), Red-edge position linear extrapolation (REP-LE), REP-Inverted Gaussian fitting technique (REP-IG), REP-Polynomial fitting technique (REP-P) and NDVI had the best relationship with chlorophyll and nitrogen contents. The first derivative of reflectance, between 560 and 760 nm, transformed by the normal state variable (SNV) also had highly significant correlation coefficients with the N, Chl, and yield. Additionally, the corn yield showed highly significant correlations with the N and Chl contents. From the point of view of the diagnosis of the nutritional status of corn, the spectral indices and REP values were suitable for establishing the nutritional status of corn in relation to N in the phenological stages V8 and R1.
通过对玉米叶片反射率与叶绿素(Chl)和氮(N)含量的关系进行分析,以确定以氮为指标诊断作物营养状况的有用光谱指标。在4个重复试验中,随机选取5个氮肥处理(0、50、100、150、200 kg ha-1),利用FieldSpec 4型光谱辐射仪在3个物候阶段测量叶片的光谱响应。利用各种方法计算了几种光谱指数和红边位置(REP)值。红边位置线性插值法(REP-L)、红边位置线性外推法(REP-LE)、rep -倒高斯拟合法(REP-IG)、rep -多项式拟合法(REP-P)和NDVI与叶绿素和氮含量的关系最好。在560 ~ 760 nm范围内,由正态变量(SNV)变换的反射率一阶导数也与氮、Chl和产率呈极显著相关。玉米产量与氮、氯含量呈极显著相关。从诊断玉米营养状况的角度来看,光谱指标和REP值适合于确定玉米在物候期V8和R1与N有关的营养状况。
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引用次数: 1
Effect of plant growth promoting bacteria on the phenology of the Amarilla maranganí quinoa cultivar 促生菌对藜麦maranganí品种物候的影响
Pub Date : 2022-01-01 DOI: 10.17584/rcch.2022v16i1.13440
Jeimmi Liliana Amado-Rodríguez, Narda Paola Humeje-Pan, Jorge Orlando Blanco-Valvuena, Nidia Yaneth Torres-Merchán, M. García-Parra
Bacteria associated with plant roots can generate different responses on the growth and development of plants which affect yield. For this reason, a test was conducted and aimed at evaluating the effects of plant growth promoting bacteria’s inoculation on the yield of the Amarilla Maranganí quinoa cultivar, using bacterial strains such as Bacillus macerans, Bacillus laterosporus, Bacillus licheniformis, Bacillus cereus, Actinobacillus, Pseudomonas aeruginosa, Consortia (a combination of the characterized bacteria), and DIPEL (Bacillus thuringensis var. Kurstaki). The study included the evaluation of the length of the plants and panicles as well as the number of inflorescences and seed production using a completely randomized experimental design. The results showed that the microorganisms had a large impact on plant growth. Actinobacillus increased the number of panicles while P. aeruginosa improved grain production. These results allowed us to confirm that the use of microorganisms favors the growth parameters of quinoa and allowed us to recognize the biological potential of growth promoting bacteria in this crop under conditions of poor water and nutrient availability.
与植物根系相关的细菌可以对植物的生长发育产生不同的反应,从而影响产量。为此,本研究利用芽孢杆菌、芽孢杆菌、地衣芽孢杆菌、蜡样芽孢杆菌、放线菌杆菌、铜绿假单胞菌、联合体菌(所鉴定细菌的组合)和DIPEL(苏云金杆菌var. Kurstaki)等菌株,对接种促生菌对Amarilla Maranganí藜麦品种产量的影响进行了研究。本研究采用完全随机试验设计,对植株长度、穗长、花序数和种子产量进行了评价。结果表明,微生物对植物生长有较大的影响。放线菌增加了穗数,铜绿假单胞菌提高了粮食产量。这些结果使我们确认微生物的使用有利于藜麦的生长参数,并使我们认识到在水分和养分供应不足的条件下,藜麦作物中促进生长的细菌的生物学潜力。
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引用次数: 0
Heterotic groups of onions (Allium cepa L.) for the development of low-pungency hybrids 洋葱(Allium cepa L.)低刺激性杂交种发育的杂种优势群
Pub Date : 2022-01-01 DOI: 10.17584/rcch.2022v16i1.13584
Fátima Rosangela de Souza Saraiva, R. D. Melo, P. H. Cerutti, J. Coimbra, D. P. Alves, A. F. Guidolin
The growing demand for low-pungency cultivars onion has opened new market niches. The objective of this study was to characterize heterotic onion groups to develop low-pungency hybrid cultivars. A field experiment with a randomized block design and two factors (genotype and fertilization) arranged in split plots was carried out in Ituporanga-SC (Brazil), in 2019. The plots corresponded to two fertilization levels (with and without sulfur), and the subplots corresponded to 26 onion genotypes, where the germplasm was derived from different (male-sterile-LA and maintainer-LB) lines, advanced populations and cultivars. The bulbs were evaluated for the following traits: pyruvic acid (indicator of pungency), sulfur content and bulb yield. The genotype performance differed according to the fertilization level. In the sulfur-free treatments, four heterotic groups were identified. The genetic variation in the crosses LA11 × LB24 and LA15 × LB19 could be exploited for negative selection for pungency and positive selection for bulb yield.
对低刺激性洋葱品种的需求不断增长,开辟了新的市场。本研究的目的是对洋葱杂种优势群体进行特征鉴定,以培育低刺激性的杂交种。2019年,在巴西Ituporanga-SC进行了随机区组试验,并将两个因素(基因型和施肥)分块布置。这些样地对应两个施肥水平(含硫和不含硫),亚样地对应26个洋葱基因型,其中的种质来自不同的(雄性不育系- la和保持系- lb)系、先进群体和栽培品种。对球茎进行了以下性状评价:丙酮酸(辛辣指标)、硫含量和球茎产量。不同施肥水平的水稻基因型表现不同。在无硫处理中,鉴定出4个杂种群体。LA11 × LB24和LA15 × LB19杂交组合的遗传变异可用于颖颖性的负选择和鳞茎产量的正选择。
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引用次数: 0
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Revista Colombiana de Ciencias Hortícolas
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