Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990253
Mahdee Samae, Somyot Chirasatitsin
Hemagglutination is a specific form of agglutination that involves red blood cells (RBCs) and antibody used for blood typing testing. Some conventional testing of blood typing has a restriction in manual observation. In this study, a simple prototype of a disposable plastic microfluidic chip coupled with a pair of coplanar electrodes made of titanium resistant wires was fabricated to investigate the degree of agglutination based on electrical impedimetric measurement. The charge transfer resistance in the impedimetric detection was used for the analysis of agglutination level. This proposed approach provided quantitative classification of agglutination level from non-agglutination (level 0) to strong agglutination (level 4+) with small amount of whole blood sample (43% Hematocrit).
{"title":"The Degree of Hemagglutination on a Plastic Microfluidic Chip Using Impedimetric Detection","authors":"Mahdee Samae, Somyot Chirasatitsin","doi":"10.1109/BMEiCON47515.2019.8990253","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990253","url":null,"abstract":"Hemagglutination is a specific form of agglutination that involves red blood cells (RBCs) and antibody used for blood typing testing. Some conventional testing of blood typing has a restriction in manual observation. In this study, a simple prototype of a disposable plastic microfluidic chip coupled with a pair of coplanar electrodes made of titanium resistant wires was fabricated to investigate the degree of agglutination based on electrical impedimetric measurement. The charge transfer resistance in the impedimetric detection was used for the analysis of agglutination level. This proposed approach provided quantitative classification of agglutination level from non-agglutination (level 0) to strong agglutination (level 4+) with small amount of whole blood sample (43% Hematocrit).","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131009740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990303
Jaroonrut Prinyakupt, Thanakorn Yootho
This paper aims to design and construction of the palm and foot warmers for pediatric patients, who are in shock situation in the ICU. The designed device was used to reducing the risk of tissue deficiency in the palms and feet according to the requirements of the Queen Sirikit National Institute of Child Health (Children's Hospital). This device acted as a hot air generator, controlled the temperature of the hot air send to the pediatric patient's gloves and socks, which serves to store hot air. The working temperature was in the range of 36.5°C to 37.5°C. This device consists of 2 main parts: 1) hot air generator consisting of air pump, heater chamber, solid-state relay, electromechanical relay, temperature sensors, microcontroller, over temperature protection circuit (40°C), display and alarm. The program control was written with C language and 2) gloves and socks were made from black cotton fabric and connected to the silicone rubber hose. The other end of the hose was connected to the hot air generator. The performance testing on this device according to the standard calibration devices composed of: 1) the results of the internal temperature of gloves and socks testing with the Fluke 289 True RMS Multimeter found that it can control the temperature in the gloves and socks at 36.5°C to 37.5°C after warming up the device for 50 minutes and 60 minutes respectively, according to doctor's requirements and 2) electrical leakage testing results with the Fluke ESA 612 Electrical Safety Analyzer found that a maximum Average leakage current was 85.30μA within the standard recommended by IEC 60601-1 (IEC: International Electrotechique Commission) was not more than 500μA.
本论文旨在为ICU中处于休克状态的儿科患者设计和制作暖掌、暖足器。根据诗丽吉王后国家儿童健康研究所(儿童医院)的要求，设计的装置用于减少手掌和脚部组织缺乏的风险。该装置作为热空气发生器，控制热空气的温度，将热空气送到患儿的手套和袜子中，起到储存热空气的作用。工作温度范围为36.5 ~ 37.5℃。本装置主要由2个部分组成:1)热风发生器由气泵、加热室、固态继电器、机电继电器、温度传感器、单片机、过温保护电路(40℃)、显示和报警组成。程序控制是用C语言编写的，手套和袜子是用黑色棉织物制成的，并连接到硅胶软管上。软管的另一端连接到热风发生器上。本设备的性能测试按标准校准装置组成:1)用Fluke 289 True RMS万用表测试手套和袜子内部温度的结果发现，分别加热50分钟和60分钟后，可以将手套和袜子内部温度控制在36.5℃至37.5℃;根据医生的要求和2)用Fluke ESA 612电气安全分析仪的漏电测试结果发现，最大平均漏电电流为85.30μA，在IEC 60601-1 (IEC:国际电工委员会)推荐的标准内不超过500μA。
{"title":"Design and Construction of the Prototype Gloves and Socks for Temperature Control in the Palms and Feet of Children with Shock Condition","authors":"Jaroonrut Prinyakupt, Thanakorn Yootho","doi":"10.1109/BMEiCON47515.2019.8990303","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990303","url":null,"abstract":"This paper aims to design and construction of the palm and foot warmers for pediatric patients, who are in shock situation in the ICU. The designed device was used to reducing the risk of tissue deficiency in the palms and feet according to the requirements of the Queen Sirikit National Institute of Child Health (Children's Hospital). This device acted as a hot air generator, controlled the temperature of the hot air send to the pediatric patient's gloves and socks, which serves to store hot air. The working temperature was in the range of 36.5°C to 37.5°C. This device consists of 2 main parts: 1) hot air generator consisting of air pump, heater chamber, solid-state relay, electromechanical relay, temperature sensors, microcontroller, over temperature protection circuit (40°C), display and alarm. The program control was written with C language and 2) gloves and socks were made from black cotton fabric and connected to the silicone rubber hose. The other end of the hose was connected to the hot air generator. The performance testing on this device according to the standard calibration devices composed of: 1) the results of the internal temperature of gloves and socks testing with the Fluke 289 True RMS Multimeter found that it can control the temperature in the gloves and socks at 36.5°C to 37.5°C after warming up the device for 50 minutes and 60 minutes respectively, according to doctor's requirements and 2) electrical leakage testing results with the Fluke ESA 612 Electrical Safety Analyzer found that a maximum Average leakage current was 85.30μA within the standard recommended by IEC 60601-1 (IEC: International Electrotechique Commission) was not more than 500μA.","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"54 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134127371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990203
S. Umchid, Pateemoh Samae, Suprakorn Sangkarak, Thidarat Wangkram
The transportation of blood is one of the most important issues in the hospital and healthcare sector since the blood bags have to be kept within a certain range of the temperatures for the entire travel time in order to maintain blood quality during transportation. Therefore, the objective of this work is to design, develop and construct a temperature controlled blood bank transport cooler to maintain the properties of blood for transportation within the hospital. The developed blood bank transport cooler uses thermoelectric peltier cooler to create coolness and a microcontroller with Proportional-Integral-Derivative (PID) controller is used to control the temperature in the blood bank cooler within the range between 2°C and 8°C. The current temperature in the cooler is monitored on the LCD display and the notification as light and sound will be activated when the current temperature is out of the desired range. To verify the performance of the developed blood bank cooler, the temperature measured from the temperature sensor of the developed blood bank is compared with the temperature measured from the temperature calibrator. The results show that the measurement errors are within ±10.984%, which is in the acceptable range according to the standards of the World Health Organization (WHO).
{"title":"Design and Development of a Temperature Controlled Blood Bank Transport Cooler","authors":"S. Umchid, Pateemoh Samae, Suprakorn Sangkarak, Thidarat Wangkram","doi":"10.1109/BMEiCON47515.2019.8990203","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990203","url":null,"abstract":"The transportation of blood is one of the most important issues in the hospital and healthcare sector since the blood bags have to be kept within a certain range of the temperatures for the entire travel time in order to maintain blood quality during transportation. Therefore, the objective of this work is to design, develop and construct a temperature controlled blood bank transport cooler to maintain the properties of blood for transportation within the hospital. The developed blood bank transport cooler uses thermoelectric peltier cooler to create coolness and a microcontroller with Proportional-Integral-Derivative (PID) controller is used to control the temperature in the blood bank cooler within the range between 2°C and 8°C. The current temperature in the cooler is monitored on the LCD display and the notification as light and sound will be activated when the current temperature is out of the desired range. To verify the performance of the developed blood bank cooler, the temperature measured from the temperature sensor of the developed blood bank is compared with the temperature measured from the temperature calibrator. The results show that the measurement errors are within ±10.984%, which is in the acceptable range according to the standards of the World Health Organization (WHO).","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114848436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990243
Phitsini Suvarnaphaet, Suvicha Sasivimolkul, Chayanisa Sukkasem, Danai Pukesamsombut, N. Tanadchangsaeng, S. Boonyagul, Suejit Pechprasarn
A biomedical electrode patch for detecting an electrocardiogram signal which is provoked by electrical activity through the heart has been investigated. The electrode patch was made of chemical derived graphene as an electrically conductive layer and polyhydroxyalkanoate (PHA) as a flexible substrate. This gra-phene/PHA patch has been promised to be completely biocom-patible and biodegradable by microbes in terrestrial environments. According to the fabrication of the electrode patch, gra-phene was synthesized using Hummers’ method and reduction with hydrazine hydrate. The PHA membrane, an aliphatic polyester bioplastic, was accumulated by Ralstonia eutropha and then was casted using electrospinning technique to serve nanofiber scaffold for an abundance of graphene to be addressed. The gra-phene/PHA electrodes were interfaced to 3-lead electrocardiogram (ECG) sensor and amplifier modules controlling by computing microcontroller. Real-time in vitro monitoring of the simulated ECG signals were observed, including normal condition at 60 BPM and abnormal heart rhythms, through the interfaces of graphene/PHA electrodes. The signals have been shown obviously and no degradation over time, however, the signal attenuation might increase due to in vivo measurement of human skin-electrode impedance.
{"title":"Biodegradable Electrode patch made of Graphene/PHA for ECG detecting Applications","authors":"Phitsini Suvarnaphaet, Suvicha Sasivimolkul, Chayanisa Sukkasem, Danai Pukesamsombut, N. Tanadchangsaeng, S. Boonyagul, Suejit Pechprasarn","doi":"10.1109/BMEiCON47515.2019.8990243","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990243","url":null,"abstract":"A biomedical electrode patch for detecting an electrocardiogram signal which is provoked by electrical activity through the heart has been investigated. The electrode patch was made of chemical derived graphene as an electrically conductive layer and polyhydroxyalkanoate (PHA) as a flexible substrate. This gra-phene/PHA patch has been promised to be completely biocom-patible and biodegradable by microbes in terrestrial environments. According to the fabrication of the electrode patch, gra-phene was synthesized using Hummers’ method and reduction with hydrazine hydrate. The PHA membrane, an aliphatic polyester bioplastic, was accumulated by Ralstonia eutropha and then was casted using electrospinning technique to serve nanofiber scaffold for an abundance of graphene to be addressed. The gra-phene/PHA electrodes were interfaced to 3-lead electrocardiogram (ECG) sensor and amplifier modules controlling by computing microcontroller. Real-time in vitro monitoring of the simulated ECG signals were observed, including normal condition at 60 BPM and abnormal heart rhythms, through the interfaces of graphene/PHA electrodes. The signals have been shown obviously and no degradation over time, however, the signal attenuation might increase due to in vivo measurement of human skin-electrode impedance.","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"208 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124672744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990358
Artit Rittiplang, P. Phasukkit
Multiple human detection through the wall has become an interesting topic for security, rescue, life detecting under earthquake rubble, etc. This paper presents a UWB radar at 3 GHz for detecting multiple humans through the wall based on Doppler frequency and variance statistic of the respiratory signal. Technically, we have referred to efficiently simple methods are FFT and variance statistic for identifying the respiratory frequency of multiple persons quickly. Experimental results show the methods can identify and evaluate quickly respiratory frequencies of two persons through the wood wall.
{"title":"UWB Radar for Multiple Human Detection Through the Wall Based on Doppler Frequency and Variance Statistic","authors":"Artit Rittiplang, P. Phasukkit","doi":"10.1109/BMEiCON47515.2019.8990358","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990358","url":null,"abstract":"Multiple human detection through the wall has become an interesting topic for security, rescue, life detecting under earthquake rubble, etc. This paper presents a UWB radar at 3 GHz for detecting multiple humans through the wall based on Doppler frequency and variance statistic of the respiratory signal. Technically, we have referred to efficiently simple methods are FFT and variance statistic for identifying the respiratory frequency of multiple persons quickly. Experimental results show the methods can identify and evaluate quickly respiratory frequencies of two persons through the wood wall.","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"187 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121306744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990212
Pornthep Sarakon, H. Kawano, S. Serikawa
Surface-Defect segmentation plays an important role. It is very necessary to detect on products during production process. Though there are several previous works in surface-defect segmentation, it needs high handcraft skill. We address automatic segmentation algorithm in surface using U-shape inverted residuals to achieve end-to-end learning network. A proposed method is data acquisition, surface-defect segmentation network creation and training. First, the experimental image is augmented by image processing technique, such as rotation, flip, translation, skew and zoom in, which is randomly augmented. Second, U-shape inverted residuals segmentation network is created by changing backbone of encoder and reconstructs decoder by inverted of encoder in order to improve performance of segmentation network. In the final step, the training step of the proposed network is set. To evaluate the performance of the proposed network, each plastic hose tip and dental caries 10,000 image are used to compare between proposed network and Unet [15]. From the experiment, Dice score and IoU are 77.11% and 62.75% in plastic hose tip, respectively. In dental caries problem, Dice score and IoU are 84.16% and 72.65%, respectively. The results show that the proposed network is satisfactory and able to be improved for higher performance. Advantages of the method are that it avoids handcraft feature extraction and is automatically learning.
{"title":"Surface-Defect Segmentation using U-shaped Inverted Residuals","authors":"Pornthep Sarakon, H. Kawano, S. Serikawa","doi":"10.1109/BMEiCON47515.2019.8990212","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990212","url":null,"abstract":"Surface-Defect segmentation plays an important role. It is very necessary to detect on products during production process. Though there are several previous works in surface-defect segmentation, it needs high handcraft skill. We address automatic segmentation algorithm in surface using U-shape inverted residuals to achieve end-to-end learning network. A proposed method is data acquisition, surface-defect segmentation network creation and training. First, the experimental image is augmented by image processing technique, such as rotation, flip, translation, skew and zoom in, which is randomly augmented. Second, U-shape inverted residuals segmentation network is created by changing backbone of encoder and reconstructs decoder by inverted of encoder in order to improve performance of segmentation network. In the final step, the training step of the proposed network is set. To evaluate the performance of the proposed network, each plastic hose tip and dental caries 10,000 image are used to compare between proposed network and Unet [15]. From the experiment, Dice score and IoU are 77.11% and 62.75% in plastic hose tip, respectively. In dental caries problem, Dice score and IoU are 84.16% and 72.65%, respectively. The results show that the proposed network is satisfactory and able to be improved for higher performance. Advantages of the method are that it avoids handcraft feature extraction and is automatically learning.","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"534 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123066794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990229
Chinakorn Sujimongkol, Suntharee Wichakhreuang, Pattaraporn Wongput, S. Daochai
BACKGROUND: Peroxyacetic acid solution (PAA) has come into widespread use as a dialyzer germicide after the hemodialyzer reprocessing and reusing has been frequently practiced in many settings globally. A recirculation technique which is an existing rinsing preferred by users to obliterate residual chemical for the pre-dialysis process. However, there is uncertainty whether with and without recirculation is most effective due to a paucity of high-quality data. OBJECTIVE: To assess the rinsing techniques whether remaining chemical has been gotten rid of properly from the hemodialyzer among different manners. METHODS: A crossover design was used to determine whether the non-recirculation technique is equivalent to the recirculating technique for efficient clearance of the chemical debris in a dialyzer. Reused dialyzers were the randomized objects for the experiment, retrieved from enrolled patients who were treated with hemodialysis. The orderings of the rinsing procedure were randomised into two arms (recirculating versus non-recirculating techniques). The main measures of technique performance were peracetic residuals. RESULTS: A total of 24 reused hemodialyzers were recruited from chronic hemodialysis patients and randomised, 12 to each arm in which without carryover effect were aliased in each other. Nevertheless, using a non-recirculating method decreased the odds of having germicide (PAA) residues by roughly 7% (p > 0.10). The results from both equivalence tests were statistically equivalent. A peak level of PAA was detected in the time-series at 10 minute with given a beta coefficient of 1.15 (95% CI [0.22-2.09]). These values indicated rebound effects. CONCLUSION: The capacity to elimination of PAA residuals of the non-recirculating technique was as effective as that of the recirculation technique despite rebound effects.
{"title":"Randomized Controlled Crossover Trial to Evaluate the Efficacy of Rinsing Techniques for Removal of Disinfectant in Hemodialyzer","authors":"Chinakorn Sujimongkol, Suntharee Wichakhreuang, Pattaraporn Wongput, S. Daochai","doi":"10.1109/BMEiCON47515.2019.8990229","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990229","url":null,"abstract":"BACKGROUND: Peroxyacetic acid solution (PAA) has come into widespread use as a dialyzer germicide after the hemodialyzer reprocessing and reusing has been frequently practiced in many settings globally. A recirculation technique which is an existing rinsing preferred by users to obliterate residual chemical for the pre-dialysis process. However, there is uncertainty whether with and without recirculation is most effective due to a paucity of high-quality data. OBJECTIVE: To assess the rinsing techniques whether remaining chemical has been gotten rid of properly from the hemodialyzer among different manners. METHODS: A crossover design was used to determine whether the non-recirculation technique is equivalent to the recirculating technique for efficient clearance of the chemical debris in a dialyzer. Reused dialyzers were the randomized objects for the experiment, retrieved from enrolled patients who were treated with hemodialysis. The orderings of the rinsing procedure were randomised into two arms (recirculating versus non-recirculating techniques). The main measures of technique performance were peracetic residuals. RESULTS: A total of 24 reused hemodialyzers were recruited from chronic hemodialysis patients and randomised, 12 to each arm in which without carryover effect were aliased in each other. Nevertheless, using a non-recirculating method decreased the odds of having germicide (PAA) residues by roughly 7% (p > 0.10). The results from both equivalence tests were statistically equivalent. A peak level of PAA was detected in the time-series at 10 minute with given a beta coefficient of 1.15 (95% CI [0.22-2.09]). These values indicated rebound effects. CONCLUSION: The capacity to elimination of PAA residuals of the non-recirculating technique was as effective as that of the recirculation technique despite rebound effects.","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131074658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.1109/BMEiCON47515.2019.8990316
S. Nuh, A. Kwanyuang, N. Konthapakdee, Somyot Chirasatitsin, Tonghathai Phairatana
Electrochemical sensors and biosensors have been focused on the development of a microfluidic integrated on a screen-printed electrode (SPE) for use in medical diagnosis. Polydimethylsiloxane (PDMS), an elastomer material, has been widely used for the fabrication of microfluidic devices. Regarding PDMS-based microfluidic integrated on SPE, there is a significant issue of leakage, especially for a continuous monitoring system. In this study, we aim to investigate the effect of PDMS ratio on PDMS-based microfluidic with serpentine pattern integrated commercial SPEs to overcome the leakage problem when using in a continuous flow system. The mechanical properties of different PDMS ratios, including 10:1, 10:0.8, 10:0.6, 10:4, and 10:0.2 were examined in terms of elastic modulus using an Instron universal testing machine. Additionally, leakage test was performed by continuous flow through microfluidic devices prepared in different PDMS ratios and integrated directly onto a commercial SPE. The results revealed that leaks occurred easily when using the microfluidic device fabricated with the standard PDMS mixing ratio at 10:1, whereas the more flexible PDMS microfluidic device at a ratio of 10:0.6 provides a suitable ratio with an excellent performance of leakage-free. This indicates that a stiff PDMS-based microfluidic device can cause leakage more easily than a flexible PDMS-based microfluidic device. Hence, the PDMS ratio of 10:0.6 exhibits a great promise in fabricating a microfluidic integrated on commercial SPE toward the development of microfluidic-based electrochemical sensors and biosensors for use in a continuous monitoring system.
{"title":"Leakage-Free Bonding of a Serpentine PDMS Microfluidics Directly on a Screen-Printed Electrode","authors":"S. Nuh, A. Kwanyuang, N. Konthapakdee, Somyot Chirasatitsin, Tonghathai Phairatana","doi":"10.1109/BMEiCON47515.2019.8990316","DOIUrl":"https://doi.org/10.1109/BMEiCON47515.2019.8990316","url":null,"abstract":"Electrochemical sensors and biosensors have been focused on the development of a microfluidic integrated on a screen-printed electrode (SPE) for use in medical diagnosis. Polydimethylsiloxane (PDMS), an elastomer material, has been widely used for the fabrication of microfluidic devices. Regarding PDMS-based microfluidic integrated on SPE, there is a significant issue of leakage, especially for a continuous monitoring system. In this study, we aim to investigate the effect of PDMS ratio on PDMS-based microfluidic with serpentine pattern integrated commercial SPEs to overcome the leakage problem when using in a continuous flow system. The mechanical properties of different PDMS ratios, including 10:1, 10:0.8, 10:0.6, 10:4, and 10:0.2 were examined in terms of elastic modulus using an Instron universal testing machine. Additionally, leakage test was performed by continuous flow through microfluidic devices prepared in different PDMS ratios and integrated directly onto a commercial SPE. The results revealed that leaks occurred easily when using the microfluidic device fabricated with the standard PDMS mixing ratio at 10:1, whereas the more flexible PDMS microfluidic device at a ratio of 10:0.6 provides a suitable ratio with an excellent performance of leakage-free. This indicates that a stiff PDMS-based microfluidic device can cause leakage more easily than a flexible PDMS-based microfluidic device. Hence, the PDMS ratio of 10:0.6 exhibits a great promise in fabricating a microfluidic integrated on commercial SPE toward the development of microfluidic-based electrochemical sensors and biosensors for use in a continuous monitoring system.","PeriodicalId":213939,"journal":{"name":"2019 12th Biomedical Engineering International Conference (BMEiCON)","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131719657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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