Russian Journal of Infection and Immunity最新文献

{"title":"Assessment of protein s and c level and its relationship with health-related factors in people living with hiv: a cross-sectional study","authors":"E. Rezaei, Elham Jamali, Z. Foroozanfar, F. Ataei, S. Beheshti, H. Joulaei","doi":"10.15789/2220-7619-aop-2629","DOIUrl":"https://doi.org/10.15789/2220-7619-aop-2629","url":null,"abstract":"Introduction. Not only does Human Immunodeficiency Virus (HIV) threaten the complications associated with immunodeficiency, but also does it cause a set of chronic conditions that may lead to serious problems in these patients. Hypercoagulable state and other hematologic manifestations are reported as leading factors in various clinical problems like deep vein thrombosis in People Living with HIV (PLHIV). The present study aimed to investigate whether there is any correlation between proteins S and C levels (the thrombophilic conditions in HIV seropositive cases) and hematological factors, biochemical markers, CD4 count, HIV viral load, anti-retroviral therapy, Hepatitis C (HCV) and hepatitis B (HBV) confection, drug use, infectious diseases, and demographic characteristics. \u0000Materials and methods. Protein S and C levels in 100 PLHIV were measured. Coagulation tests, CD4 count, HIV viral load, biochemical and hematological factors, and infectious tests were measured in these cases to assess any possible correlation between these factors and the patients proteins S and C levels. \u0000Results. Protein S, and C deficiency among PLHIV 8% and 10%, respectively. Red blood cell, hemoglobin, hematocrit, fasting blood sugar, and albumin were directly related to protein S, and the patients with positive VDRL significantly had a lower level of protein S. The patients receiving anti-retroviral therapy and those with positive VDRL had a higher level of protein C. CD4 count, prothrombin time, and cholesterol had also a direct correlation with protein C level. \u0000Conclusion. According to our results and the reduction of protein S, protein C, and the other factors affecting the lifestyle of PLHIV, there is an urge to pay special attention to thromboembolic disease. Moreover, there is a more possibility of hemostatic imbalances and coagulation disorders in them.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83544590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative characteristics of nasal mucosa microflora in various forms of chronic rhinitis","authors":"O. Smirnova, N. Goncharova","doi":"10.15789/2220-7619-cco-8056","DOIUrl":"https://doi.org/10.15789/2220-7619-cco-8056","url":null,"abstract":"Dysbiosis of the nasal mucosa contributes to the development of secondary immune disorders, maladjustment and contributes to the development of pathological processes, regardless of the phenotype of chronic rhinitis. The aim of our work was to comparatively assess microbial species and quantitative composition as well as frequency of its occurrence in patients with various forms of chronic rhinitis. We present the results of a laboratory examination of patients with chronic rhinitis (79 subjects aged 18 to 70 years), including 20 patients with chronic allergic,20 with chronic vasomotor,18 with chronic atrophic,21 with chronic infectious rhinitis. The control group consisted of 40 apparently healthy donors. All patients underwent nasal mucosa bacteriological examination. Statistical analysis of the obtained results was carried out using the Statistica for Windows 8.0 software package (StatSoft Inc., USA,2008). Nonparametric KruskalWallis, MannWhitney, and Wilcoxon tests were used to assess differences in groups. The critical level of statistical significance in testing scientific hypotheses was considered equal to p 0.05. In the control group, microorganisms of the genera Staphylococcus, Streptococcus, Neisseria, Acinetobacter, Klebsiella, Proteus are detected in the microflora of the nasal mucosa, which do not cause pathological changes. An interesting fact is the detection of Proteus mirabilis and Acinetobacter baumannii in healthy volunteers in the absence of clinical manifestations of infection, which proves the possibility of coexistence with opportunistic flora while maintaining the immune system functionining. In chronic allergic rhinitis, microorganisms of the genera Staphylococcus, Streptococcus, Neisseria, Enterobacter, Сorynebacterium are detected. In chronic vasomotor rhinitis, representatives of the genera Staphylococcus, Streptococcus, Neisseria, Klebsiella are detected. In chronic atrophic rhinitis, representatives of the genera Staphylococcus, Streptococcus, Neisseria, Acinetobacter are detected, with a predominance of Klebsiella bacteria. In chronic infectious rhinitis, representatives of the genera Staphylococcus, Streptococcus, Neisseria, Acinetobacter, Escherichia, Enterobacter, Corynebacterium, Pseudomonas, Proteus, Candida are detected. The absence and/or decrease in the number of representatives of the normoflora was found identifying pathogenic and opportunistic bacteria. With inflammation in the mucous membrane of the nasal cavity, regardless of its etiology, there is a change in the microflora quantitative and species composition. The most pronounced dysbiosis of the nasal mucosa microflora was found in chronic infectious rhinitis.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85771728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular genetic characterization of the epstein–Barr Virus: a relationship with the clinical features of pediatric infectious mononucleosis","authors":"M. I. Popkova, O. Utkin, E. Filatova, D. Bryzgalova, Nikolai A. Sakharnov, Evgeniya A. Soboleva, L.V. Nazarova","doi":"10.15789/2220-7619-mgc-2121","DOIUrl":"https://doi.org/10.15789/2220-7619-mgc-2121","url":null,"abstract":"Introduction. Infectious mononucleosis (IM) is an high priority viral infection in children. Epstein-Barr virus (EBV) is the main etiological agent of IM. EBV is classified into two main types EBV-1 and EBV-2. In addition, different variants of the virus are isolated based on individual genes, among which the LMP-1 gene and the oncoprotein it encodes are the most well known. So far, the study of the clinical significance of EBV genetic diversity in EBV-IM in children in Russia has not been conducted. The aim of the study was to evaluate a relationship between EBV LMP-1 molecular genetic variants and clinical and laboratory manifestations of IM in children. \u0000Materials and methods. The material of the study was presented by blood leukocyte and saliva samples of children aged 117 years with EBV-IM (n = 69). A total of 132 EBV isolates were studied. For differential detection of EBV-1/EBV-2, we used a previously optimized one-round PCR variant with electrophoretic detection of amplification products in agarose gel. The nucleotide sequences of the C-terminal region of the LMP-1 gene were determined by Sanger sequencing followed by analysis of the obtained sequences using the MEGA X software. Multiple Factor Analysis was used to search for the relationship between LMP-1 variants and clinical and laboratory manifestations of IM (32 signs and 8 groups of signs). \u0000Results. It was established that only one type of virus, EBV-1, was identified in all children. At the same time, the severity of clinical manifestations of EBV-IM in children varied significantly (from 15.5 to 35.5 scores in total). Molecular genetic analysis of the sequences of the LMP-1 C-terminal region in Nizhny Novgorod region EBV isolates demonstrated a significant heterogeneity of the viral population, which was not limited only to their grouping according to known variants. According to the frequency of detection, B95-8 was the dominant variant of LMP-1 (60.66.0% of cases), other variants were less common (China 1, NC, Med and China 1+В95-8). It was found that EBV-IM proceeded more easily and with less severity of the intoxication syndrome in cases of infection with a virus having the molecular genetic profile of EBV-1/B95-8, in particular EBV-1/B95-8/E214D. Conversely, EBV-1/Med, as well as EBV-1/Med/L338S, EBV-1/Med/S229T, EBV-1/China 1/L338S and EBV-1/NC/S229T profiles were associated with more severe infection. \u0000Conclusion. For the first time, the influence of the genetic diversity of EBV on the clinical manifestations of IM in children was revealed. In the context of the tasks to be solved in this study, it is necessary to conduct a larger-scale and systemic studies in different territories of Russia.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83913522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-COVID-19 complications in kidney transplant recipients","authors":"Mojtaba Shafiekhani, Sara Abolghasemi, Masoumeh Asgharpour, Zahra Zare, Halimeh Negahban, R. Akbari, H. Nikoupour, J. Roozbeh, Farshid Oliaie, Y. Yahyapour, Z. Geraili, M. Barari, S. Ebrahimpour, M. Baziboroun","doi":"10.15789/2220-7619-pcc-7111","DOIUrl":"https://doi.org/10.15789/2220-7619-pcc-7111","url":null,"abstract":"Background. Although most people recover from acute COVID-19 within a few weeks, some have long-lasting clinical problems. The prevalence of these prolonged complications in kidney transplant (KT) recipients has not been determined. \u0000Materials and methods. Six months following of 148 patients with post-COVID-19 syndrome admitted to three centers in Iran (Tehran, Shiraz and Babol) that underwent KT were included in this study. Also, one-hundred COVID-19 patients without KT were included as the control group. The demographic data, medications, and disease course were recorded. The baseline and demographic characteristics were analyzed using the Chi-square test. Moreover, students t-test were utilized to compare case and control groups. \u0000Results. The total number of patients was 248, of which 148 were in the case groups. Hospitalization associated with COVID-19 was for all patients; besides, there were 18 patients in control and 24 case groups admitted to an intensive care unit (ICU). The most commonly reported symptom was fever. Multivariate analysis identified the history of chronic kidney disease, hypertension, cerebral vascular accident, and diabetes mellites as predictors for developing post-COVID clinical complications. \u0000Conclusion. Evidence shows the high commonness of post-COVID-19 syndrome among kidney transplant patients after COVID-19, and the most common symptoms were fever, chills, and myalgia. So, all patients recovered from acute COVID-19 should undergo long-term monitoring to evaluate and treat possible complications.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75730681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Various reagent kits for comparatively analyzed effectiveness of humoral immune response after vaccination “Sputnik V\"","authors":"S. P. Kazakov, D. Reshetnyak, N. Davydova, Оksana А. Efimushkina, S. Putkov","doi":"10.15789/2220-7619-vrk-1977","DOIUrl":"https://doi.org/10.15789/2220-7619-vrk-1977","url":null,"abstract":"Modern methods of vaccination have confirmed their high effectiveness in fighting against the pandemic of a novel coronavirus infection. However, the use of various types of vaccines requires studying the effectiveness of the post-vaccination immune response (PIO), first of all, assessing the specificity and amount of production of antibodies to the surface and internal antigens of the SARS-CoV-2 virus, in particular, after the use of the most promising domestic vaccine Gam-Covid-Vac (Sputnik V). In particular, the data on the effectiveness of PVIR (increased antibody level) for the most promising domestic vaccine Gam-Covid-Vac (Sputnik V) need to be confirmed. It is also necessary to evaluate the effectiveness of the laboratory methods used for this purpose. To assess the effectiveness of PVIR, samples of biomaterial (blood serum) obtained on the 2225 day after inoculation of the second component of the vaccine from 202 Sputnik V-vaccinated, not preimmunized subjects, lacking antibodies to SARS-CoV-2 before vaccination, were examined. The levels of IgG and IgM antibodies to the surface and nucleocapsid antigens of the SARS-CoV-2 virus were determined using several semi-quantitative and quantitative methods and reagent kits from national and foreign manufacturers. To assess the diagnostic effectiveness of the methods, a comparative analysis of the specificity and sensitivity of all the laboratory methods used and a data correlation for various quantitative methods (using the Spearman correlation coefficient) between antibodies specific to the full-sized trimerized S-protein and the receptor-binding domain (RBD) of the SARS-CoV-2 S-protein were carried out. During the study, it was determined that all the reagent kits used to study IgG antibodies against SARS-CoV-2 S-protein and S-protein RBD showed maximum (100%) specificity in in the majority subjects (from 186 to 202 samples). The relevance of the data (comparative specificity), both for semi-quantitative and quantitative ELISA as well as for both ILA test systems regarding qualitative characteristics (positivenegative) was also close to the maximum magnitude (98.499.4%). A post-vaccination immune response during Sputnik V vaccination analyzing production of specific IgG antibodies was observed in 98.9% of all vaccinated subjects. IgG antibody range was studied to assess related protective level. Data are presented that a protective level of more than 150 BAU/мл was observed in 59.390.5% of the subjects, above 500 BAU/мл in 22.052.4% of the subjects, depending on the method used and the set of reagents. Based on the data obtained, it is concluded that all the methods and kits of reagents used, including domestically manufactured semi-quantitative tests, can be considered adequate to assess effectiveness of the post-vaccination immune response analyzing production of SARS-CoV-2-specific antibodies.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84192345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method for identification of acinetobacter baumannii bv. tryptophandestruens and its subbiovars A and B","authors":"E. P. Sivolodskii, L. Kraeva, E. Melnikova, G. Gorelova","doi":"10.15789/2220-7619-mfi-9379","DOIUrl":"https://doi.org/10.15789/2220-7619-mfi-9379","url":null,"abstract":"The aim of the study was to increase diagnostic sensitivity for identification of A. baumannii bv. tryptophandestruens bacteria and assess prevalence of this biovar and its subbiovars among A. baumannii clinical isolates. There were examined 210 primary strains of A. baumannii isolated in 20212022 at Bacteriological Laboratory of the Military Medical Academy named after S.M. Kirov, of which 42 strains were A. baumannii bv. tryptophandestruens. Tryptophandestruens biovar bacteria were identified by chromogenic biotransformation of sodium benzoate on a dense nutrient medium (g/l): peptone enzymatic 5.0; NaCl 5.0; FeCl3 6H2O (10% aqueous solution) 0.11 ml; bromothymol blue (1.6% aqueous solution) 3 ml; agar 15.0; NaOH (4% solution) 2.63 ml; distilled water 1 l; all components were dissolved by heating and added with sodium benzoate (CAS 532-32-1) 1.02.0; adjusted pH 7.20.2; sterilized at 121С, poured into Petri dishes. Bacteria of subbiovars A and B of biovar tryptophandestruens were identified by chromogenic biotransformation of L-tryptophan using the same nutrient medium supplemented with L-tryptophan (1.02.0 g/l) instead of sodium benzoate. Both nutrient media were used simultaneously. The A. baumannii cultures studied were seeded with a loop on the media sectors in the form of a plaque, incubated aerobically at 37С for 1824 hours, and analyzed final data as follows: the presence of a dark brown color zone of the nutrient medium around bacterial lawn on sodium benzoate- and L-tryptophan-containing medium indicated detection of subbiovar A of the tryptophandestruens biovar; in case of dark brown zone on sodium benzoate- but not L-tryptophan-containing medium around bacterial lawn identified biovar tryptophandestruens subbiovar B. The study revealed for the first time the chromogenic biotransformation of sodium benzoate (benzoic acid) and its importance as a marker for the biovar tryptophandestruens A. baumannii. Two subbiovars A and B of the tryptophandestruens biovar were found. A method was developed to identify the biovar tryptophandestruens A. baumannii and its subbiovars A and B by chromogenic biotransformation of sodium benzoate and L-tryptophan, which enhances diagnostic sensitivity by detecting the subbiovar B. The frequency of tryptophandestruens biovar distribution among primary clinical isolates of A. baumannii in 20212022 was determined: out of 210 strains of A. baumannii were 42 (20.03.5%) strains of bv. tryptophandestruens including subbiovar A 27 (12.92.3%), subbiovar B 15 (7.11.7%).","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81794001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal allergy as a risk factor for developing rosacea","authors":"Anna A. Barilo, S. Smirnova","doi":"10.15789/2220-7619-faa-8038","DOIUrl":"https://doi.org/10.15789/2220-7619-faa-8038","url":null,"abstract":"The pathogenesis of rosacea is not fully understood. In this disease, the release of inflammatory molecules and overgrowth of skin-colonizing microorganisms, including fungal microbiota, are noted. The study of the frequency of occurrence and the nature of the spectrum of sensitization to fungal allergens in patients with rosacea is of particular interest and accounts for relevance of the study. Purpose of the study: to analyze the frequency of occurrence and the nature of the spectrum of sensitization to fungal allergens in patients with rosacea. \u0000Materials and methods. The study included patients with rosacea aged 18 to 60 years (group 1, n = 31). All patients underwent a specific allergic examination: collected allergic history, skin prick testing using the following allergens: nutritional yeast, Candida albicans, Alternaria alternate, Aspergillus fumigatus, Cladosporium herbarum, Penicillium notatum (Allergopharma, Germany). Concentration of total immunoglobulin E (IgE) and eosinophilic cationic protein in blood serum was measured in indirect immunofluorescence analysis with Thermo Scientific Multiskan FC semi-automatic analyzer. Statistica 8.0 software package was used for statistical analysis. \u0000Results. Analysis of the features of the spectrum of sensitization to fungal allergens in the group of patients with rosacea showed that the most significant allergens are Cladosporium herbarum (74.2% of patients) and Candida albicans (58.1%). Sensitization to nutritional yeast and Penicillium notatum was detected in almost half of the patients. The concentration of total immunoglobulin E in blood serum in the group of patients with rosacea was 79.4 IU/ml [23.4; 105.1], in the control group 45.1 IU/ml [23.4; 144.0]. The concentration of eosinophilic cationic protein in blood serum in the group of patients with rosacea was significantly higher compared to the control group: 50.3 ng/ml [43.0; 62.0] and 7.9 ng/ml [4.6; 27.1], respectively. After skin prick testing with fungal allergens, patients with rosacea were prescribed an individual elimination diet with the exclusion of causally significant allergens for a period of 1 month. In 61.2% (n = 19) of cases in patients with rosacea, a positive elimination effect was noted: a significant decrease in the severity of erythema, papules and pustules in the face. \u0000Conclusion. Thus, the study revealed a high incidence of fungal allergy and established the features of the spectrum of sensitization to fungal allergens in patients with rosacea. The most significant fungal allergens in rosacea are fungi of the genus Cladosporium herbarum, Candida albicans, Penicillium notatum as well as nutritional yeast.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75787797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disciples of Pasteur from Russia - 2","authors":"M. M. Karapats, Ludmila Kraeva","doi":"10.15789/2220-7619-dop-9638","DOIUrl":"https://doi.org/10.15789/2220-7619-dop-9638","url":null,"abstract":"In 2022, a global scientific community noted the 200th anniversary of the outstanding scientist, one of founders of a science of microbiology Louis Pasteur. Pasteur's works promoted formation of the microbic theory and gave a momentum to a search of ways for prevention of infections, including hospital ones. Among Pasteur's disciples and colleagues are E.Ru, I.I.Mechnikov as well as many Russian scientists. Not all family names of Pasteur's disciples were revealed to a wide range of readers. Much of them returned home and to bring a marked contribution to development of microbiology and immunology in Russia. Here, we continue seek out and return biographies of the Russian scientists - Louis Pasteur's disciples. \u0000Among Russian scientists who passed school of Pasteur Institute there may be mentioned many graduates of one of the oldest medical institutions of Russia Military Medical academy as well as associates of I.I.Mechnikov and N.F.Gamalei J.J. Bardach, S.I.Zlatogorov, N.I.Chistovich, L.A.Tarasevich; renowned surgeons - V.A.Ratimov and V.A.Oppel, one of founders of Pasteur Institute in St.-Petersburg - P.P.Maslakovets. Many Pasteur's followers took part in creating the institute resembling Pasteur Institute nowadays known as the Institute of Experimental Medicine. In the latter regard, we mention E.F.Shperk, V.I.Nedrigajlov, etc. Moreover, it is also necessary to note the first woman in Russia Doctor P.V.Tsiklinskuju who was awarded a title Professor of Bacteriology. \u0000Pasteur's disciples received theoretical and practical knowledge from their teacher and returned to own Fatherlands to fully serve with full dedication. They brought with new methods of scientific research and diagnostics of infectious diseases, used already known immune serums and vaccine preparations and developed their own similar agents to combat infections that were relevant in those years: rabies, plague, cholera, typhoid fever, tuberculosis. \u0000All Russian scientists, disciples of Louis Pasteur as well as its friend and colleague I.I.Mechnikov continued to serve to a society owing to great achievements in the field of microbiology by transferring own knowledge and experience to numerous disciples - continuers of work of the great scientist Louis Pasteur. Many of them were immortalized in the names of research institutes, departments and laboratories.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"10 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82706002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FEATURES OF NK CELL PHENOTYPE VIRUS GENOTYPE-DRIVEN CHRONIC VIRAL HEPATITIS C","authors":"A. Savchenko, E. P. Tikhonova, A. Anisimova, I. Kudryavtsev, Vasilij Belenjuk, A. Borisov","doi":"10.15789/2220-7619-hcv-8047","DOIUrl":"https://doi.org/10.15789/2220-7619-hcv-8047","url":null,"abstract":"Elimination of the hepatitis C virus (HCV) due to direct antiviral drug (DAD) action affects alteration in virus phenotype and, accordingly, NK cell functional activity. However, the published data are very contradictory. \u0000Aim. Investigating alterations in NK cell subset phenotype after DAD treatment of HCV genotype-dependent chronic viral hepatitis C (CVHC) patients. \u0000Material. 111 CVHC patients and 21 healthy volunteers were examined. The diagnosis was established on epidemiological, clinical and laboratory data. All 111 subjects with CVHC received direct antiviral drugs Sofosbuvir and Velpatasvir for 12 weeks. The study of the NK cell phenotypes wwas analyzed by multicolor flow cytometry. \u0000Results. A decreased count of cytokine-producing along with increased frequency of cytotoxic NK cells were found in CVHC patients blood samples with various HCV genotypes prior to DAD treatment. The imbalance of cytotoxic cells with a high level of functional activity was also found in CVHC patients regardless of HCV genotype. The patients with HCV genotypes 1 and 3 showed significantly increased level of immunoregulatory NK cells. In addition, increased count of glycohydrolase (CD38) and ecto-5-nucleotidase (CD73)-expressing NK cells were found in patients with HCV genotypes 1 and 3. Hence, such alterations in NK cell phenotype in CVHC patients were presented as sustained high viral load which peaking at carriers of HCV genotype 1 that was minimal in patients with HCV genotype 2. The most prominent change in NK cells after DAD treatment was found in CVHC patients with HCV genotype 2 (normalization of CD8-expressing NK cell subset composition and count). Only patients with HCV genotype 2 after treatment had increased frequencies of peripheral blood double-negative CD38CD73 NK cells. Patients with HCV genotypes 1 and 3 also showed minimally improved in NK cell subset composition after DAD treatment. \u0000Conclusions. Evaluation of specific changes in NK cell phenotype during DAD treatment of CVHC patients driven by HCV genotype undoubtedly is of importance and high relevance. The results obtained are novel and complement the insights into CVHC immunopathogenesis. Analysis of NK cell phenotypes and functional activity in patients with CVHC may promote development of new methods for treating HCV infection.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88476292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Level of Vitamin D in Preterm Infected and Uninfected Infants","authors":"H. Boskabadi, M. Zakerihamidi, Raheleh Faramarzi","doi":"10.15789/2220-7619-cot-8050","DOIUrl":"https://doi.org/10.15789/2220-7619-cot-8050","url":null,"abstract":"Introduction: Despite the substantial progress in intensive cares, sepsis is still an important cause of neonatal mortality. Given the role of vitamin D in infection control; therfore, this study was conducted to compare vitamin D level in infected and uninfected preterm infants. \u0000Materials and Methods: This cross-sectional study was carried out on 87 preterm infants(45 infected infants and 42 uninfected infants) hospitalized in Mashhad Ghaem Hospital,Iran, during 2015-2017. . The subjects were selected by using convenience sampling. . The infected infants (n=45) included babies with clinical and laboratory findings compatible with infection and/or positive blood or cerebrospinal fluid cultures. The serum levels vitamin D were measured in all infants. A researcher-made questionnaire containing demographic, clinical and laboratory features of infants was used. In addition, independent t-test and chi-square test were applied. SPSS was used to perform the statistical data analysis. . \u0000Results: 83% of infants had Vitamin D deficiency 34.5%, 26.4%, and 21.8% of whom exhibited severe (less than 10ngml), moderate (10.1-20ngml), mild deficiency (20.1-30ngml), respectively. The mean vitamin D level of infants was 23.319.40ng/ml in the control group and 11.028.64ng/ml in the case group (P=0.000). In the case group, the mean vitamin D was 8.145.53ngml in early sepsis and 12.629.75ngml in late-onset sepsis (P=0.121). 95% of infected infants and 71% of uninfected infants had vitamin D less than 30ngml (P=0.003). \u0000Conclusion: Vitamin D deficiency is very common in preterm infants. Serum vitamin D levels in infants with sepsis were lower than those in uninfected infants. Therefore, the correction of Vitamin D deficiency may contribute to better control of neonatal infection.","PeriodicalId":21412,"journal":{"name":"Russian Journal of Infection and Immunity","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85172783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}