Vidyodaya Journal of Science最新文献

{"title":"Climate Smart Agriculture: The Role of Fertilizer Innovations and Efficient Plant Nutrient Management","authors":"Sayani Nimanka, N. Fernando, Madhavi de Silva, I. Munaweera, N. Kottegoda","doi":"10.31357/vjs.v1is1.6709","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6709","url":null,"abstract":"Climate change has emerged as a significant threat to the global agro-economy, adversely affecting agricultural productivity, food security, and local and global agricultural development goals. To ensure sustainable crop productivity and meet future demands, it is essential to take prompt actions to address the current challenges of lower agricultural productivity due to climate change. Climate-smart agriculture (CSA) is a novel concept that offers a promising approach to tackle these challenges by introducing sustainable farming practices that minimize negative environmental impacts and enhance resilience to climate change. It involves realigning current agricultural practices and introducing smart techniques to reduce the adverse effects of agriculture that contribute to climate change and higher carbon footprints. The importance of climate-smart fertilizers in agricultural productivity cannot be overemphasized, as the soil is susceptible to structural changes and alterations in nutrient availability due to climatic impacts. Therefore, the implementation of proper nutrient management mechanisms and advanced fertilizer innovations has become a top priority in implementing the concepts of CSA. Consequently, there has been a greater focus on introducing more climate-adaptive and resilient plant nutrient delivery systems and technologies in recent times. For instance, nanofertilizer, bio-based controlled-release and slow-release fertilizers have provided a more sustainable and climate-friendly alternative to traditional synthetic fertilizers. Despite the innovations, challenges remain in implementing CSA practices and practices of climate-smart fertilizers at scale. This is largely attributed to the lack of proper knowledge and a streamlined policy framework on climate resilience in agriculture and fertilizers. This article seeks to review the role of fertilizer innovations and nutrient management in CSA, targeting the reduction of nutrient losses due to climate changes. It also examines the current status of climate-smart fertilizers with a particular emphasis on the current implementation challenges and future prospects in fertilizer production, leading to global food security by adapting to climatic changes. By highlighting specific examples of modern climate-smart agriculture and  fertilizer management, this article aims to provide insights into the potential benefits and challenges of implementing CSA practices and encourage further research and development in this important field. Keywords: Climate-smart agriculture, Climate-smart fertilizers, Climate change, Nutrient Management, Innovations in fertilizers","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139213628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Functionalized Mesoporous Silica Nanoparticles for Enhanced Removal of Heavy Metals: A Review","authors":"Aseni Sahasri Pathiraja, I. Munaweera, N. Kottegoda","doi":"10.31357/vjs.v1is1.6705","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6705","url":null,"abstract":"Human health and environmental sustainability are strongly influenced by the contamination of water resources with hazardous heavy metal ions due to the accumulation in human body via food chains. Thereby, researchers’ attention has been paid on effective methods for heavy metal ion scavenging to prevent them releasing to environment. Notably, Mesoporous Silica Nanoparticles (MSNPs) with high surface area, massive surface area to volume ratio, large pore volume and uniform pore distribution play a crucial role in addressing this challenge. Additionally, researchers focus on novel surface functionalization methods of MSNPs with suitable organic and inorganic moieties to amplify the adsorption efficiency of heavy metals. MSNPs possess easily functionalizable surface which facilitates the modifications and enhanced removal of heavy metals. The review article summarizes the different moieties used for functionalization of MSNPs such as amino, thio, carboxyl, phenyl, cyano groups, different types of polymers, inorganic functional groups. Further, a comparison has been made between functional and unmodified MSNPs to elaborate how these modifications have enhanced the removal performance of heavy metals in water. Further, this review provides an overview on different synthesis routes and structure directing agent used in synthesis of MSNPs. Moreover, pH effect on adsorption andreusability of modified NPs, while illustrating the mechanism of adsorption on to modified MSNPs surface has also been elaborated. Maximum adsorption capacity of each functional moiety has been taken into consideration with the aim of supporting future advancements. Keywords: Adsorption, Mesoporous silica nanoparticles, Heavy metals, Functionalization, Maximum adsorption capacity","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139214924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on the Comparative Analysis of Synthetic Insect Repellents and Essential Oil Based Sustained-release Insect Repellent Formulations","authors":"S. D. M. Chinthaka, C. J. Delpagodage, A. G. W. U. Perera","doi":"10.31357/vjs.v1is1.6716","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6716","url":null,"abstract":"The necessity of developing insect repellents with highly effective sustained release properties has attracted more attention due to the vast spread of arthropod-borne diseases such as dengue and malaria, which must be controlled with proper measures, and also due to the devastating damage caused by severe infestation of insect pests on crops and stored food. Natural and synthetic insect repellents are used against insect vectors and insect pests. Topical insect repellent formulations should be less toxic, less irritant, and less skin permeable. Irrespective of the type and form of the repellent, the mechanism of action depends on the formation of a vapour barrier/ odour barrier, that would deviate the insect from reaching the host. Synthetic insect repellents are highly efficient but due to their high toxicity, degradation resistance, and bio accumulation, they raise environmental and ecological issues. Due to the adverse effects caused by synthetic insect repellents, more attention is given to insect repellents consisting of natural products.Essential oils are among the widely used natural insect repellents available due to their biocompatibility and non-toxicity. High volatility of essential oils is the major problem that hampers the application of essential oils as insect repellent agents. The high volatility that reduces the length of application, and its activity can be successfully addressed by the formulation of slow-release insect repellent composites by incorporating essential oils into adsorbing or encapsulating matrices. Zeolites, montmorillonite, β-cyclodextrin, polymeric materials, and electrospun nanomaterials are commonly used to develop slow-release formulations with essential oils. Among these, polymeric microcapsules are the most extensively studied and developed slow-release insect repellent systems that are based on natural and synthetic active compounds. Key words: Formulation, Insects, Microcapsules, Repellents, Zeolite","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139210226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advancements in Chitosan-based Adsorbents for Hexavalent Chromium Removal from Aqueous Media","authors":"Harini Methma Perera, A. Rajapaksha, Sudantha Liyanage","doi":"10.31357/vjs.v1is1.6710","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6710","url":null,"abstract":"Contamination of aqueous environments by Cr(VI) has become a matter of concern owing to its detrimental impacts on human health with its long-term exposure. Thus, effective treatment of adulterated aqueous media is critical in terms of the health and safety of humans together with flora and fauna. Currently, chitosan is considered an excellent adsorptive material for theremediation of Cr(VI) contamination owing primarily to its biodegradability, non-toxicity, abundance, and ability to modify its microstructure. The present review focuses on the up-to-date progression of chitosan-based sorbents that can be utilized in the mitigation of Cr(VI) oxyanions from aqueous media. This paper provides an overview of pristine chitosan along with recentadvancements and insights into structurally and chemically modified chitosan. Chitosan has been chemically modified through cross-linking, grafting, and/or combination with other adsorptive materials to enhance its performance in Cr(VI) removal. Structurally modified chitosan-based hybrid materials that are commonly used in Cr(VI) removal include magnetic adsorbents,hydrogels, aerogels, and nano/microparticles. The sorption capacities of chitosan-based hybrid materials have varied from 27.25 - 357.14 mg g-1 depending on the type of adsorbent, dosage, initial Cr(VI) concentration, pH, and type of modification. Also, beneficial information through a compare-and-contrast of the effectiveness of the stated sorbent materials and their variants in themitigation of Cr(VI) is provided. Furthermore, mechanisms of Cr(VI) removal by chitosan-based sorbents accentuating the main governing mechanism, electrostatic interactions are described and discussed. Desorption and regeneration studies are presented to assess the reusability of the chitosan-based adsorptive materials utilized in the mitigation of Cr(VI) contamination. Desorptionstudies reveal that sorption of Cr(VI) onto most of the chitosan-based adsorbents are fairly reversible with desorbed percentages above 60% with the usage of an efficient stripping agent. Through the literature survey of approximately 100 recently published papers, it could be evinced that chitosan-based adsorbents have proved to be an outstanding sorbent even if some challengesremain. Keywords: Adsorption, chitosan, Cr(VI), hybrid materials, mechanism","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139210535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complexes of N, N- and N, N, N- Sulfonamide Ligands as Therapeutic and Diagnostic Agents","authors":"Dilmi Witharana, T. Perera","doi":"10.31357/vjs.v1is1.6707","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6707","url":null,"abstract":"Ethylenediamine, diethylenetriamine and dipicolylamine have been used as the carrier ligands to synthesize bidentate (N,N) or tridentate (N,N,N) chelate systems that coordinate with metal centers. The terminal amine groups of ethylenediamine and diethylenetriamine and the central amine group of dipicolylamine can be easily substituted to give sulfonamide ligands having bulky aromatic fragments. In the formation of metal sulfonamides, the sulfonamide nitrogens of primary or secondary sulfonamidesdeprotonate and coordinate with metal centers forming M-N bonds while the free amines coordinate to metal centers through lone pairs. The reported synthetic approaches and the properties of these complexes and ligands are discussed in this review. The bulky sulfonamide moieties bring about unique biological features to the ligand system while enhancing the fluorescent properties of the ligand. The metal center itself incorporates interesting biological features to the complex. These properties of the metal center andthe ligand show a synergistic effect in the complexes that may come in handy when designing therapeutic or diagnostic agents. The increased lipophilicity of the ligands and thereby the complexes will ensure better uptake by target cells making them ideal candidates for biological applications. Keywords: Sulfonamide, ethylenediamine, diethylenetriamine, dipicolylamine","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139212584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Methods and Regulative Viewpoint of Antimicrobial Preservatives in Cosmetics","authors":"Manavi Perera, B. A. Perera","doi":"10.31357/vjs.v1is1.6711","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6711","url":null,"abstract":"Cosmetics need to be resistant to microbial contamination to protect consumer health and increase shelf life, much like any other product containing water, organic and inorganic components. The aims of anti-microbiological activity are to protect consumers from potentially harmful bacteria and to preserve products subject to degradation. Chemical, physical, or physicochemical methods are used to ensure this. Organic acid preservatives, alcohols, formaldehyde releasers, halogenated preservatives, isothiazolinones, quaternary ammonium salts and chlorhexidine are among the preservatives included in the legislation. Indeed, high quantitiesare more successful from a preservation standpoint, nevertheless they are toxic to consumers, whereas low amounts can lead to microbial resistance. Accordingly, the criteria of several international legislation and validation methods for introducing microbiologically safe items to the market have become essential. Although there are many approaches based on gas chromatography (GC) as per literature, the most common methods for the determination of preservatives are based on liquid chromatography (LC). Both of these procedures, as well as capillary electrophoresis (CE) and micellar electro kinetic chromatography (MEKC), have been frequently utilized in the cosmetics industry to determine preservative levels. Analytical approaches have been primarily focused on parabens, whereas the number of available methods to investigate other preservatives is limited. There is a tendency toward the usage of miniature extraction processes where new and improved sample preparation and extraction techniques including matrix solid-phase dispersion, solid-phase extraction, pressurized liquid extraction/supercritical fluid extraction and microextraction-based method have been introduced with high levels of efficiency and extraction capacities. Considering the significance and relevance of preservatives in cosmetics, this study highlights the most recent state-of-the-art information on their safety and regulatory concerns. Given the rising influence on consumer health, sample preparation and analytical methods forpreservative detection were also investigated which have been proposed by the international scientific literature. Keywords: Preservatives, Cosmetics, Analytical Techniques, Derivatization, Extraction","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"62 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139209955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Applications of Electrospun Nanofibers in Agriculture","authors":"Manesha Fernando, Dr. Imalka Munaweera, Prof. Nilwala Kottegoda","doi":"10.31357/vjs.v1is1.6702","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6702","url":null,"abstract":"Some of the major challenges associated with current agricultural practices include inefficient delivery and utilization of agrochemicals; fertilizers, pesticides, and pheromones; to crops. This results in low nutrient utilization efficiency with respect to applied fertilizers which leads to a greater economic burden to farmers to maintain crop yields at optimum levels. In addition, plant diseases by various pathogens also pose a threat to agriculture. In an effort to address some of the aforementioned challenges, electrospun nanofibers have emerged as a potential class of one-dimensional nanomaterials for use in agricultural applications. Unique characteristics of electrospun nanofibers such as enhanced surface area: volume ratio, high porosity distribution, and increased specific surface area pave the potential for agricultural applications that will be elaborated in this review. These applications include slow-release of fertilizers, pheromones and insecticides, seed and fruit coatings, plant protection, and nanofiber fabricated sensors. In addition, this review also focuses briefly on other preparation methods of nanofibers, and most importantly on the parameters that govern the electrospinning process; solution parameters, processing parameters, and ambient parameters. Furthermore, many more unexplored applications in the field of agriculture employing nanofiber usage exist, and it is believed that a greater understanding of the current nanofiber research and practices of green electrospinning will enable the upliftment of current boundaries to enable agricultural applications of nanofibers on a commercial scale. Keywords: nanofiber - agriculture - electrospinning - slow-release – sensor","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"2020 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139210756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme Related Synthesis of Lipids and Fabrication of Planar Lipid Membranes","authors":"M.A.A. Aqeel, Suneth P Rajapaksha","doi":"10.31357/vjs.v1is1.6713","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6713","url":null,"abstract":"Biological membranes are structurally and functionally complex supramolecular assemblies. The intricate complexity itself poses a significant barrier in investigating the constituents, components, and their structure-activity relationships. Scientists employ twosynthetic methods to reduce complexity: the top-down approach and the bottom-up approach. The top-down synthetic approach involves genome reduction of the living cell, leading to a collateral reduction in membrane complexity. In contrast, the bottom-upsynthetic approach like chemical and enzymatic synthesis engages assembly of non-living components to construct biomimetic membrane structures with desired complexity. The synthetic routes catalyzed by enzymes are referred to as enzymatic synthesis, while routes utilizing a combination of catalytic systems and enzymes are known as chemo-enzymatic synthesis. The lipids prepared by enzymatic or chemo-enzymatic method are essential to integrate into a membrane in a manner that makes them amenable to various analytical techniques. Planar lipid bilayer method is one such versatile technique which can be used to prepare membranes from individual lipid molecules to utilize in various analytical procedures. Two planar lipid membranes types, black lipid membrane and supported lipid membrane, are more common than other types due to their less complexity in preparation. This review discusses the enzyme related synthesis of lipids, the method of preparation and challenges involved in fabricating black lipid membrane and supported lipid membrane models.","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139211247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal Dipicolylamines and their Biomedical Applications: A Mini Review","authors":"Taneesha Baduraliyage, T. Perera","doi":"10.31357/vjs.v1is1.6708","DOIUrl":"https://doi.org/10.31357/vjs.v1is1.6708","url":null,"abstract":"Research in synthetic inorganic chemistry demonstrates that metal complexes are widely utilized as therapeutic as well as diagnostic agents. Due to the coordinative saturation, substitutional inertness, and unique redox characteristics, metal polypyridyl complexes have ignited attention and have been exploited in a number of biological and biomedical fields. The polypyridyl ligand, dipicolylamine (DPA) is a symmetrical secondary amine with two pyridyl rings. Delocalization of excited electrons occurs throughout the ligand system due to its conjugated character and the metal-to-ligand charge transfer transitions, resulting in a strong fluorescence signal. The high lipophilic nature of this ligand has proven to improve metal-DPA complex absorption by cell membranes. The N–H amine group in DPA and its analogues have the additional function of allowing hydrogen bonds to form, and deprotonation of this amine has allowed the creation of mononuclear and polynuclear species, with deprotonation of the bridging amine nitrogen also playing a significant role in the coordination sequence. DPA-appended metal complexes have garnered attention in the quest for linkages between magnetic, spectroscopic, structural, and coordination geometries. A number of mononuclear metal-DPA complex crystal structures with four, five, or six coordinated metal centers have been synthesized and explored for their biological properties. This study reviews DPA-derivatized ligand-linked metal complexes as promising cancer, microbial, and fungal inflammatory therapeutics, as well as potential diagnostic agents for fluorescence imaging and radiopharmaceuticals. Keywords: Dipicolylamine, metal complexes, coordination geometries, fluorescent agents","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"175 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139215131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, characterization and identification of cellulase (Endo-β-1,4-glucanase) producing bacteria from diverse locations","authors":"Kularajany Niranjan, Kapilan Ranganathan, Neelamanie Yapa","doi":"10.31357/vjs.v26i01.6413","DOIUrl":"https://doi.org/10.31357/vjs.v26i01.6413","url":null,"abstract":"\u0000The objective of the study was to isolate cellulase producing bacteria from natural sources and identify the bacterial isolates that produce thermostable enzyme. Bacteria were isolated from samples collected from different locations like mango leaf litter, termite gut, composting yard (municipal solid waste, compost, and soil), soil from decaying paper waste, decaying wood, and saline coastal belt soil by enrichment in broth containing 1% cellulose and subsequent culturing on plates and the purified cultures stored in slants of the solidified agar medium of the same composition as enrichment medium. Primary screening of the isolates for cellulase (Endo β-1,4-glucanase) production was done by growing them on Carboxy Methyl Cellulose (CMC) agar medium and screening using indicator dye Congo red. Secondary screening of the positive isolates showing cellulolytic Index > 4 was done by crude cellulase enzyme extraction and assay using 1% CMC in 1N citrate buffer (pH 5.0) at different pH values (4.8- 7.0) and temperatures (35- 70℃). Cellulase activity (EA) in U/mL was calculated based on the absorbance change per min. which depends on the amount of reducing sugar produced by the enzyme catalysed conversion of CMC to reducing sugar. Isolates were characterized morphologically and biochemically and six isolates (Gram -ve: T1, MSW, C1, S2 and Gram +ve: W2, P) having significantly higher EA were selected for DNA extraction. DNA was quantified and the DNA of four isolates (MSW, S2, W2, P) were selected for sequence analysis. The 16S rDNA sequencing was carried out through PCR amplification and Blast using NCBI blast similarity search tool resulted in the identification of MSW, S2, W2 as E.coli, Sulfitobacter pontiacus, Bacillus subtilis respectively and P as Bacillus cereus and Rhodococcus erythropolis strain e1. Isolates showed significantly higher cellulase activity (EA) at pH 6.2 and at varying temperatures. Bacterial isolates P and C2 showed significantly higher activity at 40℃, MSW at 60℃ and others at 50℃ (EA ranging from 0.0054 – 0.0604 U/mL). Isolates W2, P, S2 had EA ranging from 0.006 - 0.03 U/mL at 70℃. This study showed that the cellulase producers could be grown at different temperatures (40 - 70℃) on cellulose based substrate. In this study the substrate specificity indicates that the crude enzyme is an endo- β-1,4-glucanase that plays a prominent role in initiating and sustaining the hydrolytic process and randomly cleaves cellulose into glucose and olygomeric polysaccharides. Hence by selecting samples with cellulosic substance from different locations with extreme environmental conditions like high temperature, high salinity it is possible to isolate cellulolytic bacteria producing cellulase enzymes that are stable at higher processing temperatures and other harsh conditions which is of importance in industrial applications. \u0000Keywords: Endo-β-1,4-glucanase, Cellulolytic index, Cellulase activity, Thermostable","PeriodicalId":214405,"journal":{"name":"Vidyodaya Journal of Science","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132111369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}