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Space-time analysis of scientific research on Brosimum alicastrum Swartz 白菖蒲科研的时空分析
Pub Date : 2023-01-01 DOI: 10.15446/rfnam.v76n1.101008
Ezequiel Espinosa-Grande, Alberto Santillán-Fernández, Bruno Manuel Chávez-Vergara, Arely Anayansi Vargas-Díaz, Alfredo Esteban Tadeo-Noble, Jaime Bautista-Ortega
Brosimum alicastrum is a forest species of broad natural distribution in southeastern Mexico, with high potential for animal and human diets, although with incipient forest management. The objective of this study was to analyze the spatial-temporal evolution of basic and applied research where B. alicastrum was the object of study; through a bibliometric analysis of the texts available in the main editorial houses; to identify research areas that are not developed. In 308 texts found from 1883 to 2020, spatial-temporal evolution showed an exponential growth that concentrated the highest productivity from 2002 to 2020 (222 texts) in countries of the Americas. For the case of Mexico, it was found that the research was focused the southeast, which coincides with the natural distribution of the species. However, this research had a low impact (measured by the number of bibliographic citations) as a result of the publication in journals edited in Spanish, while impact journals are led by English-speaking countries, in English. Therefore, the research about B. alicastrum in Latin America has a broad margin of improvement through the publication of texts in English and in journals of greater impact, through the development of research areas that have been slightly explored such as silviculture of the species with special emphasis on its propagation, management in nursery, and forest plantations, which can contribute to food security in each country by ensuring the prime material of an emerging food agro-industry.
alicastrum Brosimum alicastrum是墨西哥东南部广泛自然分布的一种森林物种,尽管森林管理尚不成熟,但具有很高的动物和人类膳食潜力。本研究的目的是分析以苦楝为研究对象的基础研究和应用研究的时空演变;通过对主要编辑部提供的文本进行文献计量分析;找出尚未开发的研究领域。在1883 - 2020年的308篇文本中,时空演化呈指数增长,其中美洲国家在2002 - 2020年(222篇)的生产力最高。以墨西哥为例,研究的重点是东南部,这与该物种的自然分布相吻合。然而,由于该研究发表在以西班牙语编辑的期刊上,而有影响力的期刊由英语国家主导,以英语出版,因此影响较低(以文献引用次数衡量)。因此,关于拉丁美洲白菖蒲的研究有很大的改进余地,方法是在英文文本和影响力较大的期刊上发表文章,发展一些研究领域,如该物种的造林,特别强调其繁殖、苗圃管理和森林种植园,这些研究领域可以通过确保新兴食品涉农工业的主要材料,为每个国家的粮食安全作出贡献。
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引用次数: 0
Degradation dynamics of organophosphorus insecticides applied in stored soybean (Glycine max L.) during supervised trials. 贮存大豆(Glycine max L.)有机磷杀虫剂在监督试验中的降解动态。
Pub Date : 2023-01-01 DOI: 10.15446/rfnam.v76n1.102472
Julieta Strada, Cecilia Bruno, Mirtha María Nassetta, Monica Balzarini, Martha Yolanda Conles, Dante Rojas, Alejandra P. Ricca, Diego Cristos, Maria José Martinez
During storage, soybean kernels can be attacked by insects, which are preventively controlled with insecticides. Information on the dissipation of insecticide residues is crucial to know their final concentrations in food and establish the waiting periods necessary for consumption without health risks, and to determine the minimum waiting period necessary to comply with national and international standards. The aims of this study were to quantify the residue levels of organophosphorus insecticides (dichlorvos, chlorpyrifos-methyl and pirimiphos-methyl) in stored soybean, establish the effect during the storage period, and model the dissipation dynamics. Insecticide residues in soybeans were analyzed at 2, 30, 60, 90 and 120 days after application. An analytical method based on QuEChERS extraction followed by gas chromatography mass spectrometry (GC-MS/MS) determination was validated, with mean recoveries of 82-105%, depending on the spiking levels. Residues decreased below 80% of the initial concentration at 60 days after application and below quantifiable levels at 120 days. Residues followed a pseudo-first-order dissipation dynamics [Ct = C0×exp(-k.t)], with the dissipation constant (k) and half-lives being 0.538 and 1.3 days for dichlorvos, 0.018 and 38.8 days for chlorpyrifos-methyl, and 0.023 and 30.1 days for pirimiphos-methyl, respectively. These results allow concluding that, at the recommended dosage, these insecticides are safe for use on soybean grains stored under standard conditions commonly nowadays used in Argentina.
在储存过程中,大豆籽粒会受到昆虫的侵害,这需要用杀虫剂进行预防性控制。关于杀虫剂残留消散的信息对于了解其在食品中的最终浓度、确定无健康风险消费所需的等待期以及确定符合国家和国际标准所需的最短等待期至关重要。本研究旨在定量测定有机磷杀虫剂(敌敌畏、甲基毒死蜱和甲基吡虫磷)在大豆贮藏中的残留水平,确定其在贮藏期的影响,并建立其耗散动力学模型。分别在施用后2、30、60、90和120 d对大豆的农药残留进行分析。验证了基于QuEChERS萃取-气相色谱-质谱(GC-MS/MS)测定的分析方法,根据峰值水平的不同,平均回收率为82-105%。施用后60天,残留量降至初始浓度的80%以下,120天降至可量化水平以下。农药残留符合准一级耗散动力学[Ct = C0×exp(-k.t)],敌敌畏的耗散常数(k)和半衰期分别为0.538和1.3天,甲基毒死蜱的耗散常数(k)和半衰期分别为0.018和38.8天,甲基吡虫磷的耗散常数和半衰期分别为0.023和30.1天。这些结果可以得出结论,在推荐剂量下,这些杀虫剂可安全用于在阿根廷目前常用的标准条件下储存的大豆谷物。
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引用次数: 0
Bioprospecting in food production: an approximation of the current state in Colombia 粮食生产中的生物勘探:哥伦比亚现状的近似值
Pub Date : 2023-01-01 DOI: 10.15446/rfnam.v76n1.101705
Claudia Cubides, Carolina Gutiérrez Cortés, Hector Suarez
Microbial bioprospecting is the study and classification of microorganisms with industrial value. Different researches word wide are focusing on the study of natural molecules that can be used for medicine, agriculture and the environment, among others. In Colombia, marine bioprospecting has become highly relevant. Also, different universities and institutes are working on the study of the biodiversity and its applications. The aim of this review was to compilate the most important laws and decrees related with the use of the resources with commercial purposes. Also, the elucidation of the current state of bioprospecting in Colombia and the principal applications of microorganisms in the food production chain. The special focus of this review is to show the potential use of bioprospection on agricultural development of the country in order to change the conventional practices to eco-friendly process in food production. Also, this review proposes the bioprospecting of lactic acid bacteria as an alternative to use their biomass and metabolites for food preservation.
微生物生物勘探是对具有工业价值的微生物进行研究和分类。世界各地的不同研究都集中在研究可用于医学、农业和环境等方面的天然分子。在哥伦比亚,海洋生物勘探已变得十分重要。此外,不同的大学和研究机构正在开展生物多样性及其应用的研究。这次审查的目的是汇编与将资源用于商业目的有关的最重要的法律和法令。此外,还阐述了哥伦比亚生物勘探的现状以及微生物在食品生产链中的主要应用。这篇综述的特别重点是展示生物前景在该国农业发展中的潜在用途,以便在粮食生产中改变传统做法,采用生态友好的过程。此外,本文还对乳酸菌作为一种生物质和代谢物用于食品保鲜的生物资源进行了展望。
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引用次数: 0
Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate 在低成本农业基质上培养的黄曲霉纯化α -淀粉酶的催化性能
Pub Date : 2023-01-01 DOI: 10.15446/rfnam.v76n1.100842
Olushola E Owolabi, Oladipo O Olaniyi, Felix A Akinyosoye
Aspergillus flavus isolated from fermented millet flour produced a crude enzyme, which was purified via ammonium sulphate precipitation and subsequent chromatographic techniques. The biochemical characteristics of the purified amylase were thereafter investigated showing activity in a wide range of pH and temperature, with optimal conditions of pH 6.0 and 50 °C. The enzyme retained even 89% of its activity after 1 h at 50 °C and 2 h at pH 6.0. The purified enzyme was stimulated by Ca2+, Zn2+ and Co2+, while Hg2+ and EDTA caused mild inhibition of α-amylase activity. The kinetic indices (Km and Vmax) and molecular weight of the enzyme were estimated in 1.71 mg mL-1, 2.133 μmol min-1 mL-1 and 45 kDa respectively. The catalytic properties of α-amylase from A. flavus makes it a promising candidate for use in various starch processing industries.
从发酵小米粉中分离得到的黄曲霉产生了一种粗酶,通过硫酸铵沉淀和随后的层析技术对其进行了纯化。随后对纯化淀粉酶的生化特性进行了研究,结果表明该酶在较宽的pH和温度范围内具有活性,最佳条件为pH 6.0和50°C。在50°C条件下1 h,在pH 6.0条件下2 h,酶的活性仍保持89%。纯化后的α-淀粉酶受到Ca2+、Zn2+和Co2+的刺激,而Hg2+和EDTA对α-淀粉酶活性有轻微抑制作用。酶的动力学指数(Km)和分子量(Vmax)分别为1.71 mg mL-1、2.133 μmol min-1 mL-1和45 kDa。黄曲霉α-淀粉酶的催化性能使其在各种淀粉加工工业中具有广阔的应用前景。
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引用次数: 1
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Revista Facultad Nacional de Agronomía
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