Russian Journal of Immunology最新文献

{"title":"Dynamics of matrix metalloproteinases and their tissue inhibitors in patients with herpesvirus and papillomavirus infection","authors":"T. Nevezhkina, M. Chernikova, E. Markelova, M. S. Tulupova, Anna V. Kostyshko, L. Fedyanina, N. Markova","doi":"10.46235/1028-7221-9409-dom","DOIUrl":"https://doi.org/10.46235/1028-7221-9409-dom","url":null,"abstract":"Sexually transmitted infections are of great importance for the proper reproductive function in women. Chronic inflammatory process leads to reproductive disorders. A special role in the chronic inflammatory process is attributed to papillomavirus (PVI) and herpetic infection. MMP-2 and MMP-9 enzymes cleave type 4 collagen which makes the scaffold of basement membranes and contributes to the separation of endothelial cells from the membranes, followed by their further migration and direct participation in angiogenesis thus affecting the growth of tumors, in particular cervical cancer. Tissue inhibitors of matrix metalloproteinases are known to limit the collagen breakdown. However, the imbalance of MMP and TIMP is accompanied by accumulation of extracellular matrix and increased risk for reproductive disorders. The aim of our study was to evaluate the dynamics of acute-phase proteins affecting the state of intercellular matrix (MMP-2, MMP-9 and MMP tissue inhibitors (type 1, 2) in blood serum of patients with PVI or coinfection of PVI and HSV before and after therapy with drugs exhibiting antiviral and immunomodulatory effects, i.e., a synthetic compound (Inosine pranobex) and vegetable substance (Solanum tuberosum). We have examined 141 patients with papillomavirus and herpetic infections treated with Inosine pranobex and Solanum tuderosum. Determination of MMP-2, MMP-9 and TIMP-1, TIMP-2 levels of in blood serum was carried out using specific reagents from RD Diagnostics Inc. (USA). The drug therapy with active substances of Inosine pranobex and Solanum tuberosum was associated with positive dynamics of the level of MMP-2, MMP-9 and tissue inhibitors of types 1 and 2 in all groups under studies. However, Inosine Pranobex exerts more pronounced changes, especially in subgroups with viral coinfections.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89154838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T helper subsets during the acute post-traumatic period in children","authors":"R. Zakirov, S. Petrichuk, E. V. Freidlyn, Daria G. Kuptsova, O. Yanyushkina, O. Karaseva","doi":"10.46235/1028-7221-9644-tsd","DOIUrl":"https://doi.org/10.46235/1028-7221-9644-tsd","url":null,"abstract":"Severe mechanical injury is among the main reasons of disability and mortality in pediatric patients. The imbalance between the states of inflammation and immune suppression during the critical period of post-traumatic injury bears an elevated risk for infectious complications and/or multiple organ failure. The present study aimed to determine the informative immunological criteria in order to evaluate severity and prognosis for clinical outcomes in children from the severe injury group (SInj, ISS 16, n = 87); mild/moderate injury group (MInj, ISS 16, n = 34), as based on assessment of helper T cells (Th) ratios, i.e., Th17/Treg, T127hi/ Treg, and Th17/T127hi. The patients with severe injuries were classified by their outcomes (favorable, n = 47; unfavorable, n = 40), presence of infectious complications (IC, n = 16) and the development of multiple organ failure (MOF, n = 11). Th ratios were studied on the 1st, 3rd, 5th, 7th, 14th day after injury. For the Sinj group, a pronounced increase of Th17/Treg ratio in the acute post-traumatic period with a decrease by 14 days was revealed. The indices of T127hi/Treg ratio on the first day for the patients from Minj group corresponded to the values of control group and significantly differed from patients with SInj in the 3rd to 5th day. There are different kinetics of Th subset ratio in peripheral blood of children with severe injuries over time in different groups, as well as with/without MOF, in presence, or absence of infectious complications and different clinical outcomes. Significant differences in T127hi/Treg ratio level were found in group with IC from 1st to 3rd day and from 7th to 14th day. Significant differences in Th17/Treg ratio level were found in IC group (7th day), in MOF group on 14th day post-injury. The patients with MOF had lower median concentrations of Th17/Treg and T127hi/Treg than patients without MOF. The results of the study indicate that the levels of Th17/Treg and T127hi/Treg ratio in children may be used to predict outcome of the traumatic disease and assess the risk of infectious complications and multiple organ dysfunction syndrome.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74721369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of tetrapeptide Acetyl-(D-Lys)-Lys-Arg-Arg-amide on immunological and biochemical parameters of Wistar rats using passive smoking models","authors":"N. Kuzmicheva, I. V. Mikhailova, Julia V. Filippova, Aleksandr I. Smolyagin, Natalia M. Livshic, Igor V. Miroshnichenko","doi":"10.46235/1028-7221-10008-eot","DOIUrl":"https://doi.org/10.46235/1028-7221-10008-eot","url":null,"abstract":"Tetrapeptides, the homologues of adrenocorticotropic hormone fragment (15-18), play a special role among the bioregulators of the immune system. These compounds have cerebroprotective, neuroprotective and antioxidant properties. However, no available studies concerned the immunotropic properties of tetrapeptides in the models with exposure to xenobiotics, thus making such research quite relevant. Our study concerned the effects of tetrapeptide Acetyl-(D-Lys)-Lys-Arg-Arg-amide (laboratory code KK1) on the immunological and biochemical parameters of 72 female Wistar rats exposed to passive smoking. The experimental animals were fumigated with tobacco smoke for 8 hours. Synthetic peptide KK1 was administered intranasally at a dose of 40 g/kg/day five times a day for 10 days. Cotinine was detected only in blood serum of rats from experimental groups, thus confirming a contribution of this tetrapeptide to the trend for normalization of some immunological parameters in experimental animals subjected to passive tobacco smoking, expressed as an increase in thymus mass and the number of splenocytes, and a decrease in the circulating immune complexes compared to the parameters of smoking rats of the group. We revealed that passive tobacco smoking in rats was accompanied by a general tendency to accumulation of iron, lead and nickel in peripheral blood. There was a marked increase in the concentration of cadmium, lead and cobalt in rats of the experimental group compared with the content of these trace elements in the liver of smoking animals injected with tetrapeptide KK1. The revealed shifts in immunological indices may be based, firstly, on hepatotoxic effect of ecotoxicants, The lymphoid lineage is mostly affected thus causing hypoplasia of the central and peripheral immunity organs. An evident sign of such pathology is a decreased cellularity of hematopoietic and lymphoid organs found in the present study. Secondly, the tobacco smoke components with prooxidant action may disrupt cellular redox homeostasis, causing damage to cell membranes, resulting in necrosis or apoptosis, thus explaining the revealed decrease in the number of thymocytes, splenic karyocytes and a decrease in the weight of organs. Thus, our results suggest usage of experimental passive smoking in order to evaluate efficiency of the tetrapeptides. Administration of Acetyl-(D-Lys)-Lys-Arg-Arg-amide peptide to the passively smoking rats is associated with tendency to normalize the mass of the thymus and spleen, the number of thymocytes and splenocytes, and a decrease in circulating immune complexes. Further studies are required to elucidate the effects of tetrapeptides upon the immune system.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88155362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLR-9 (-1237)*T/C polymorphism in russian COVID-19 patients from the chelyabinsk region","authors":"S. Belyaeva, T. Suslova, Daria C. Stashkevich, Svetlana E. Balandina, Daria E. Mjakotina, Maria S. Milonchenko","doi":"10.46235/1028-7221-9904-tpi","DOIUrl":"https://doi.org/10.46235/1028-7221-9904-tpi","url":null,"abstract":"In COVID-19, the clinical outcome depends on a wide range of factors, including genetic features. Among them, TLRs, the genes encoding the receptors of innate immune system are of particular interest since they play the key role in development of innate immune response. The present study concerns the newely identified allelic variants of the TLR-9 (-1237)*T/C gene in Russian residents from the Chelyabinsk Region who had COVID-19 complicated by the bilateral viral pneumonia. Polymorphic variants of TLR-9 (-1237)*T/ C were determined by polymerase chain reaction. It was found that, among the COVID-19 patients, a TLR-9 allele (-1237 C) with higher transcriptional activity was more common than in the control group (19.421% and 11.275%, respectively, p = 0.019), and its homozygous genotype TLR-9 (-1237)*C was not detected in the comparison group. TLR-9 allele (-1237)*T in the patients with COVID-19 was less common in comparison with the control group (80.579% and 88.725%, respectively, p = 0.019). Taking into account the differences in suggested TLR-9 expression in more severe COVID-19 patients, we compared distribution of TLR-9 (-1237)*T/ С allele polymorphism in the patients with different severity of COVID-19. In the group of patients with mild form, the TLR-9 (-1237)*T/T genotype was more common as compared with patients who had more severe clinical course. The differences were significant at the trend level when compared with patients with a medium-severity disease (86.364% and 66,000%, respectively; p = 0.076).","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73265705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cucurbiturils on the numbers and differential counts of peripheral blood leukocytes in laboratory animals after in vivo parenteral administration","authors":"E. Pashkina, A. Aktanova, E. Kovalenko, V. Kozlov","doi":"10.46235/1028-7221-9970-eoc","DOIUrl":"https://doi.org/10.46235/1028-7221-9970-eoc","url":null,"abstract":"Immunological safety of nanoparticles is an urgent problem for development of drug delivery systems used as the basis for creating these systems. Cucurbit[n]urils (CB[n]) are molecular nanocontainers that can encapsulate various drugs and serve as the basis for delivery systems. Cucurbiturils are low-toxic compounds: under in vitro conditions, they exhibit weak immunomodulatory properties, without sufficient immunotoxicity. The aim of the present study was to evaluate the in vivo effects of cucurbiturils on the number and differential leukocyte counts in peripheral blood. \u0000BALB/c mice aged 2-4 months were used in the work. For this study, cucurbiturils were diluted in phosphate-buffered saline and administered to laboratory animals intraperitoneally (three times a week). \u0000When evaluating the effect of cucurbit[7]uril on blood parameters of the animals after three intraperitoneal injections weekly, no statistically significant changes were registered. However, at first administration, the animals showed a slight increase in relative number of lymphocytes after the first injection of CB[6], and an increased proportion of neutrophils after the first administration of CB[8]. Meanwhile, the proportions of lymphocytes and neutrophils were within normal ranges after the 2nd and 3rd injections of cucurbiturils, and did not show any significant differences against the controls. Moreover, the impact upon the subpopulation composition of peripheral blood lymphocytes in laboratory animals was assessed. After injection of CB[6], there was a decrease in T lymphocytes in the peripheral blood, along with increased proportion of CD19+B lymphocytes compared with the controls. CB[7] and CB[8] injections did not affect the subpopulation profile of peripheral blood lymphocytes. \u0000We have found that intraperitoneal administration of CB[n] did not affect the blood parameters of laboratory animals, thus, probably suggesting the in vivo safety of these compounds. At the same time, CB[6] is able to exert a stimulating effect on humoral immunity by increasing relative contents of B lymphocytes.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87472690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels of tissue inhibitor of matrix metalloproteinases type 1, matrix metalloproteinase type 7 and anthropometric parameters in practically healthy women and women with arterial hypertension in the older age group","authors":"N. S. Chepurnova, S. V. Knysh, A. Rudneva, Viktoria V. Zdor, E. Markelova, Polina V. Romanjuk, Semyon I. Sayapin","doi":"10.46235/1028-7221-9637-lot","DOIUrl":"https://doi.org/10.46235/1028-7221-9637-lot","url":null,"abstract":"Multiple studies are currently, aimed at studying the mechanisms of the development of basic age-related pathologies, e.g., arterial hypertension (AH). We found no literature sources concerning associations between the levels of matrix metalloproteinase type 7 (MMP-7), tissue inhibitor of matrix metalloproteinases type (TIMP-1) and anthropometric data which could be useful for diagnosing age-associated, socially dependent diseases and metabolic disorders. The article presents the data on the state of proteolysis system and anthropometric data of 45 almost healthy women and 45 women with arterial hypertension (AH) aged from 59 to 74 years old. The levels of MMP-7 and TIMP-1 in blood serum were studied by sandwich-variant of ELISA test. The waist and neck volume were measured three times with a centimeter tape at an accuracy of 1 cm. Statistical processing of the obtained data was performed using analytical software IBM SPSS Statistics, v. 22.0. The results show some correlations between the levels of MMP-7 and TIMP-1, and the waist and neck sizes (WC). Lower levels of MMP-7 and TIMP-1 were registered in the group of practically healthy women as compared to the second (AH) study group (p 0.01). Anthropometric data were not markedly diverse: the waist size in the group of healthy women was less than in AH group (p 0.05), and values of neck volume did not differ significantly between the groups. When assessing the relationships in the group of practically healthy women, we recorded a direct significant correlation between the waist size and neck volume (r = 0.754, p 0.0001), while in the group of women with AH this relationship was weaker (r = 0.5782, p 0.0001). In addition, a similar correlation was found between MMP-7 and TIMP-1 levels in the both groups studied. In the group of healthy women this relationship was of medium strength (r = 0.657, p 0.0001), whereas in the group of women with AH it was stronger (r = 0.720, p 0.0001). The data obtained suggest the presence of metabolic disorders in the women with arterial hypertension and signs of proteolytic enzyme system disorders.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91203246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokine profile in adolescent children with recurrent infections and polypous rhinosinusitis, ways of their correction","authors":"A. Korkmazov, E. O. Kiseleva, Violetta D. Frolova, G.D. Romanyugo","doi":"10.46235/1028-7221-7738-cpi","DOIUrl":"https://doi.org/10.46235/1028-7221-7738-cpi","url":null,"abstract":"A brief review of studies on one of the topical issues of otorhinolaryngology - polypous rhinosinusitis in people with recurrent infections is given.It was noted that patients with polyposis rhinosinusitis are more often committed to acute respiratory infections than those without nasal polyposis and they have a significant immunological imbalance.Serum levels of IL-8, IL-1, anti-inflammatory IL-4 and IFN- production were examined in 48 frequently ill patients with a history of respiratory allergopathology and polypous sinusitis.All patients, according to clinical recommendations, received complex conservative therapy.The control group consisted of 21 people who had respiratory infections no more than one or two times during the year and a history that was not aggravated by polypous rhinosinusitis.As a result, a significant decrease in IFN- levels and an increase in the concentrations of IL-8, IL-1, and IL-4 were revealed, which in turn confirms a violation of the body's immunoreactivity, a decrease in local and systemic immunity in patients with recurrent infections and polypous rhinosinusitis.The results obtained predetermine the need for the use of immunocorrective measures in the complex therapy of these patients.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75513855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significane of determining antinuclear antibodies in systemic connective tissue disorders in children","authors":"A. A. Zhuzhula, O. Kurbatova, M. Snovskaya, S. Petrichuk, T. Komyagina, Anastasia S. Tryapochkina","doi":"10.46235/1028-7221-9961-sod","DOIUrl":"https://doi.org/10.46235/1028-7221-9961-sod","url":null,"abstract":"Systemic connective tissue diseases (SCTD) are characterized by systemic autoimmune inflammation and are accompanied by development of antinuclear antibodies (ANA). Our aim was a comparative analysis of ANA in blood serum in children with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). The study included 50 patients with SLE, 50 patients with RA who were treated at the National Medical Research Center of Childrens Health. Median age was 12,2 y. o. (9-15.5). The titers of ANA and the cell fluorescence type were determined with the indirect immunofluorescence reaction (IFR) using the HEp-2 cell line (Immco Diagnostics, Inc, USA), as well as the concentration of antibodies to double-stranded DNA (adsDNA) in blood serum samples of the children detected by immunochemiluminescence (ICM) woth Elia dsDNA (Thermo Fisher Scientific, USA). A positive ANA titer and adsDNA were found, respectively, in 98% and in 48% in children with SLE. A positive ANA titer and adsDNA was detected in 100% and in 4% of children with RA, respectively. Highly positive ANA titers ( 1/1280) have been detected in 68% of children with SLE, and in 30% of children with RA. None of the RA patients with highly positive ANA titers had adsDNA. But, in patients with SLE, highly positive ANA titers and a positive dsDNA level were simultaneously detected in 16% of cases. There are both single types of cell fluorescence and their combinations in children with SLE and RA. Nuclear dot-like fluorescence was more common in children with SLE, cytoplasmic type, in children with RA, nucleolar type of glow was found only in children with RA. The revealed combinations of ANA and adsDNA titers in children with SLE and RA confirm the need for simultaneous use of RNIF and ICM.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90017993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of graphene oxide nanoparticles on apoptosis of T-lymphocytes and Jurkat cells","authors":"D. Usanina, S. Uzhviyuk, S. Zamorina","doi":"10.46235/1028-7221-9635-eog","DOIUrl":"https://doi.org/10.46235/1028-7221-9635-eog","url":null,"abstract":"Graphene and its derivatives are materials with unique physicochemical properties. A detailed study of these materials allows to consider them prospective biomedical agents for targeted drug and gene delivery, photothermal therapy of cancer, bioimaging, etc. However, this requires a comprehensive studies of their effects on the body tissues, including cells of the immune system. \u0000The aim of our research was to stydy the effects of nanoparticles based on pegylated graphene oxide (GO) upon apoptosis of T lymphocytes derived from blood of healthy donors and Jurkat 5332 cell line. Comparison of these cells will extend our knowledge of the effects of nanomaterials on the cells, and to respond the question, what results obtained with continuous cell lines are valid for normal non-malignant cells. In this work, we used GO nanoparticles (100-200 nm, 1-5 m) coated with linear (LP-GO) and branched (BP-GO) polyethylene glycol (PEG). The cells were cultured for 24 hours at 37 C and 5% CO2with nanoparticles at concentrations of 5 and 25 g/mL. Viability and early and late apoptosis of incubated Jurkat cells and CD3+cells from healthy donors were assessed by flow cytometry. It was found that the small nanoparticles coated with linear PEG at high concentrations (25 g/mL) could significantly reduce the number of live cells and increase the number of cells in late apoptosis. At the same time, large nanoparticles coated with branched PEG at high concentrations (25 g/mL) increased the percentage of T cells in early apoptosis. \u0000Meanwhile, the GO nanoparticles at both concentrations did not affect the viability and apoptosis of Jurkat cells, regardless of the size, concentration, and type of surface function of the particles. \u0000The obtained results suggest that GO nanoparticles exert different effects upon normal and malignant lymphocytes of T lineage. One may assume that these discrepancies could be explained by greater resistance of tumor cells compared to normal T cells. These findings suggests that studies of nanomaterials upon living cells should not be limited to experiments on cell lines, since their properties may significantly differ from those of non-malignant cells.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85579345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of oxidative phosphorylation and glycolysis in CD4+T lymphocytes of HIV/HCV coinfected immunological non-responders by means of the seahorse technology","authors":"L. Korolevskaya, Violetta V. Vlasova, N. Shmagel, E. V. Saidakova","doi":"10.46235/1028-7221-9936-soo","DOIUrl":"https://doi.org/10.46235/1028-7221-9936-soo","url":null,"abstract":"Oxidative phosphorylation and glycolysis are essential for CD4+ T-lymphocyte survival, division, and functioning. However, indirect evidence suggests that in HIV-positive hepatitis C virus (HCV) coinfected immunological non-responders to antiretroviral therapy, the CD4+ T-cell metabolic activity parameters are violated. This information implies that in immunological non-responders, CD4+ T-lymphocytes' inability to productively divide and increase in number after viral suppression by antiretroviral drugs may be due to metabolic dysfunction. The newly released technology for the analysis of extracellular fluxes using seahorse XF equipment permits assessment of the cells metabolic activity. The aim of this study was to evaluate the efficiency of oxidative phosphorylation and glycolysis in CD4+ T-lymphocytes of HIV/HCV coinfected immunological non-responders using Seahorse technology. Peripheral blood samples from patients of two groups were studied: HIV/HCV coinfected immunological non-responders with CD4+ T-lymphocyte count less than 350/l and HIV/HCV coinfected immunological responders with CD4+ T-cell count more than 500/l. In isolated CD4+ T-lymphocytes, the basal and maximal oxygen consumption rates by complexes of the mitochondrial electron transport chain, as well as the rate of medium acidification by protons formed during glycolysis, were assessed. It has been established that in HIV/HCV coinfected immunological non-responders, both basal and maximal oxygen consumption rates by CD4+ T-cell mitochondria are reduced. Moreover, in isolated CD4+ T-lymphocytes of immunological non-responders, the basal rate of glycolysis is increased. It can be assumed that a significant part of CD4+ T-lymphocytes in HIV/HCV coinfected immunological non-responders is activated and ready for homeostatic proliferation, which aggravates the need for additional energy and macromolecules. However, cells are unable to change their metabolism in a coordinated manner to meet these demands. The identified dysregulation of metabolic pathways may contribute to the low regenerative capacity of CD4+ T-lymphocytes in HIV/HCV coinfected immunological non-responders.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79186144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}