Pub Date : 2023-08-11DOI: 10.46235/1028-7221-8955-sia
M. Korkmazov, M. A. Lengina, I. Dubinets, Anna Yu. Kravchenko, Semyon V. Klepikov
Over the past decade, targeted therapy with various monoclonal antibodies has become particularly relevant for the treatment of chronic polypous rhinosinusitis (PR). This is primarily due to the high incidence rate, polyetiological origin and pathogenetic features of polyposis development, low effectiveness of existing treatment approaches, the tendency for relapse, and comorbid conditions. The article provides a brief historical background concerning various predictors of the mucous membrane remodeling in the nasal cavity and paranasal sinuses at the stages of the polyposis formation thus justifying the need for implementation of monoclonal antibodies in the treatment schedules. Considering the leading role of Th2-inflammation in immunopathogenesis of developing polypous vegetations, the influence of targeted therapy upon treatment of chronic polypous rhinosinusitis is theoretically evaluated, and we highlight some important issues that should be further specified. Undoubtedly, inhibition of the synthesis of necessary interleukins leads to improvement in clinical symptoms and reduced size of polypous vegetations. At the same time, the real biochemical transformations of the nasal mucosa have been scarcely studied. E.g., an attempt to inhibit some cytokine may lead to indirect blockage of other pro-inflammatory cytokines. In future, it is necessary to study the pharmacodynamics of targeted drugs in order to clarify distinct contraindications to their use.
{"title":"Some immunological aspects of targeted therapy in polypous rhinosinusitis","authors":"M. Korkmazov, M. A. Lengina, I. Dubinets, Anna Yu. Kravchenko, Semyon V. Klepikov","doi":"10.46235/1028-7221-8955-sia","DOIUrl":"https://doi.org/10.46235/1028-7221-8955-sia","url":null,"abstract":"Over the past decade, targeted therapy with various monoclonal antibodies has become particularly relevant for the treatment of chronic polypous rhinosinusitis (PR). This is primarily due to the high incidence rate, polyetiological origin and pathogenetic features of polyposis development, low effectiveness of existing treatment approaches, the tendency for relapse, and comorbid conditions. The article provides a brief historical background concerning various predictors of the mucous membrane remodeling in the nasal cavity and paranasal sinuses at the stages of the polyposis formation thus justifying the need for implementation of monoclonal antibodies in the treatment schedules. Considering the leading role of Th2-inflammation in immunopathogenesis of developing polypous vegetations, the influence of targeted therapy upon treatment of chronic polypous rhinosinusitis is theoretically evaluated, and we highlight some important issues that should be further specified. Undoubtedly, inhibition of the synthesis of necessary interleukins leads to improvement in clinical symptoms and reduced size of polypous vegetations. At the same time, the real biochemical transformations of the nasal mucosa have been scarcely studied. E.g., an attempt to inhibit some cytokine may lead to indirect blockage of other pro-inflammatory cytokines. In future, it is necessary to study the pharmacodynamics of targeted drugs in order to clarify distinct contraindications to their use.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90153333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-9409-dom
T. Nevezhkina, M. Chernikova, E. Markelova, M. S. Tulupova, Anna V. Kostyshko, L. Fedyanina, N. Markova
Sexually transmitted infections are of great importance for the proper reproductive function in women. Chronic inflammatory process leads to reproductive disorders. A special role in the chronic inflammatory process is attributed to papillomavirus (PVI) and herpetic infection. MMP-2 and MMP-9 enzymes cleave type 4 collagen which makes the scaffold of basement membranes and contributes to the separation of endothelial cells from the membranes, followed by their further migration and direct participation in angiogenesis thus affecting the growth of tumors, in particular cervical cancer. Tissue inhibitors of matrix metalloproteinases are known to limit the collagen breakdown. However, the imbalance of MMP and TIMP is accompanied by accumulation of extracellular matrix and increased risk for reproductive disorders. The aim of our study was to evaluate the dynamics of acute-phase proteins affecting the state of intercellular matrix (MMP-2, MMP-9 and MMP tissue inhibitors (type 1, 2) in blood serum of patients with PVI or coinfection of PVI and HSV before and after therapy with drugs exhibiting antiviral and immunomodulatory effects, i.e., a synthetic compound (Inosine pranobex) and vegetable substance (Solanum tuberosum). We have examined 141 patients with papillomavirus and herpetic infections treated with Inosine pranobex and Solanum tuderosum. Determination of MMP-2, MMP-9 and TIMP-1, TIMP-2 levels of in blood serum was carried out using specific reagents from RD Diagnostics Inc. (USA). The drug therapy with active substances of Inosine pranobex and Solanum tuberosum was associated with positive dynamics of the level of MMP-2, MMP-9 and tissue inhibitors of types 1 and 2 in all groups under studies. However, Inosine Pranobex exerts more pronounced changes, especially in subgroups with viral coinfections.
{"title":"Dynamics of matrix metalloproteinases and their tissue inhibitors in patients with herpesvirus and papillomavirus infection","authors":"T. Nevezhkina, M. Chernikova, E. Markelova, M. S. Tulupova, Anna V. Kostyshko, L. Fedyanina, N. Markova","doi":"10.46235/1028-7221-9409-dom","DOIUrl":"https://doi.org/10.46235/1028-7221-9409-dom","url":null,"abstract":"Sexually transmitted infections are of great importance for the proper reproductive function in women. Chronic inflammatory process leads to reproductive disorders. A special role in the chronic inflammatory process is attributed to papillomavirus (PVI) and herpetic infection. MMP-2 and MMP-9 enzymes cleave type 4 collagen which makes the scaffold of basement membranes and contributes to the separation of endothelial cells from the membranes, followed by their further migration and direct participation in angiogenesis thus affecting the growth of tumors, in particular cervical cancer. Tissue inhibitors of matrix metalloproteinases are known to limit the collagen breakdown. However, the imbalance of MMP and TIMP is accompanied by accumulation of extracellular matrix and increased risk for reproductive disorders. The aim of our study was to evaluate the dynamics of acute-phase proteins affecting the state of intercellular matrix (MMP-2, MMP-9 and MMP tissue inhibitors (type 1, 2) in blood serum of patients with PVI or coinfection of PVI and HSV before and after therapy with drugs exhibiting antiviral and immunomodulatory effects, i.e., a synthetic compound (Inosine pranobex) and vegetable substance (Solanum tuberosum). We have examined 141 patients with papillomavirus and herpetic infections treated with Inosine pranobex and Solanum tuderosum. Determination of MMP-2, MMP-9 and TIMP-1, TIMP-2 levels of in blood serum was carried out using specific reagents from RD Diagnostics Inc. (USA). The drug therapy with active substances of Inosine pranobex and Solanum tuberosum was associated with positive dynamics of the level of MMP-2, MMP-9 and tissue inhibitors of types 1 and 2 in all groups under studies. However, Inosine Pranobex exerts more pronounced changes, especially in subgroups with viral coinfections.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89154838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-10008-eot
N. Kuzmicheva, I. V. Mikhailova, Julia V. Filippova, Aleksandr I. Smolyagin, Natalia M. Livshic, Igor V. Miroshnichenko
Tetrapeptides, the homologues of adrenocorticotropic hormone fragment (15-18), play a special role among the bioregulators of the immune system. These compounds have cerebroprotective, neuroprotective and antioxidant properties. However, no available studies concerned the immunotropic properties of tetrapeptides in the models with exposure to xenobiotics, thus making such research quite relevant. Our study concerned the effects of tetrapeptide Acetyl-(D-Lys)-Lys-Arg-Arg-amide (laboratory code KK1) on the immunological and biochemical parameters of 72 female Wistar rats exposed to passive smoking. The experimental animals were fumigated with tobacco smoke for 8 hours. Synthetic peptide KK1 was administered intranasally at a dose of 40 g/kg/day five times a day for 10 days. Cotinine was detected only in blood serum of rats from experimental groups, thus confirming a contribution of this tetrapeptide to the trend for normalization of some immunological parameters in experimental animals subjected to passive tobacco smoking, expressed as an increase in thymus mass and the number of splenocytes, and a decrease in the circulating immune complexes compared to the parameters of smoking rats of the group. We revealed that passive tobacco smoking in rats was accompanied by a general tendency to accumulation of iron, lead and nickel in peripheral blood. There was a marked increase in the concentration of cadmium, lead and cobalt in rats of the experimental group compared with the content of these trace elements in the liver of smoking animals injected with tetrapeptide KK1. The revealed shifts in immunological indices may be based, firstly, on hepatotoxic effect of ecotoxicants, The lymphoid lineage is mostly affected thus causing hypoplasia of the central and peripheral immunity organs. An evident sign of such pathology is a decreased cellularity of hematopoietic and lymphoid organs found in the present study. Secondly, the tobacco smoke components with prooxidant action may disrupt cellular redox homeostasis, causing damage to cell membranes, resulting in necrosis or apoptosis, thus explaining the revealed decrease in the number of thymocytes, splenic karyocytes and a decrease in the weight of organs. Thus, our results suggest usage of experimental passive smoking in order to evaluate efficiency of the tetrapeptides. Administration of Acetyl-(D-Lys)-Lys-Arg-Arg-amide peptide to the passively smoking rats is associated with tendency to normalize the mass of the thymus and spleen, the number of thymocytes and splenocytes, and a decrease in circulating immune complexes. Further studies are required to elucidate the effects of tetrapeptides upon the immune system.
{"title":"Effect of tetrapeptide Acetyl-(D-Lys)-Lys-Arg-Arg-amide on immunological and biochemical parameters of Wistar rats using passive smoking models","authors":"N. Kuzmicheva, I. V. Mikhailova, Julia V. Filippova, Aleksandr I. Smolyagin, Natalia M. Livshic, Igor V. Miroshnichenko","doi":"10.46235/1028-7221-10008-eot","DOIUrl":"https://doi.org/10.46235/1028-7221-10008-eot","url":null,"abstract":"Tetrapeptides, the homologues of adrenocorticotropic hormone fragment (15-18), play a special role among the bioregulators of the immune system. These compounds have cerebroprotective, neuroprotective and antioxidant properties. However, no available studies concerned the immunotropic properties of tetrapeptides in the models with exposure to xenobiotics, thus making such research quite relevant. Our study concerned the effects of tetrapeptide Acetyl-(D-Lys)-Lys-Arg-Arg-amide (laboratory code KK1) on the immunological and biochemical parameters of 72 female Wistar rats exposed to passive smoking. The experimental animals were fumigated with tobacco smoke for 8 hours. Synthetic peptide KK1 was administered intranasally at a dose of 40 g/kg/day five times a day for 10 days. Cotinine was detected only in blood serum of rats from experimental groups, thus confirming a contribution of this tetrapeptide to the trend for normalization of some immunological parameters in experimental animals subjected to passive tobacco smoking, expressed as an increase in thymus mass and the number of splenocytes, and a decrease in the circulating immune complexes compared to the parameters of smoking rats of the group. We revealed that passive tobacco smoking in rats was accompanied by a general tendency to accumulation of iron, lead and nickel in peripheral blood. There was a marked increase in the concentration of cadmium, lead and cobalt in rats of the experimental group compared with the content of these trace elements in the liver of smoking animals injected with tetrapeptide KK1. The revealed shifts in immunological indices may be based, firstly, on hepatotoxic effect of ecotoxicants, The lymphoid lineage is mostly affected thus causing hypoplasia of the central and peripheral immunity organs. An evident sign of such pathology is a decreased cellularity of hematopoietic and lymphoid organs found in the present study. Secondly, the tobacco smoke components with prooxidant action may disrupt cellular redox homeostasis, causing damage to cell membranes, resulting in necrosis or apoptosis, thus explaining the revealed decrease in the number of thymocytes, splenic karyocytes and a decrease in the weight of organs. Thus, our results suggest usage of experimental passive smoking in order to evaluate efficiency of the tetrapeptides. Administration of Acetyl-(D-Lys)-Lys-Arg-Arg-amide peptide to the passively smoking rats is associated with tendency to normalize the mass of the thymus and spleen, the number of thymocytes and splenocytes, and a decrease in circulating immune complexes. Further studies are required to elucidate the effects of tetrapeptides upon the immune system.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88155362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-9904-tpi
S. Belyaeva, T. Suslova, Daria C. Stashkevich, Svetlana E. Balandina, Daria E. Mjakotina, Maria S. Milonchenko
In COVID-19, the clinical outcome depends on a wide range of factors, including genetic features. Among them, TLRs, the genes encoding the receptors of innate immune system are of particular interest since they play the key role in development of innate immune response. The present study concerns the newely identified allelic variants of the TLR-9 (-1237)*T/C gene in Russian residents from the Chelyabinsk Region who had COVID-19 complicated by the bilateral viral pneumonia. Polymorphic variants of TLR-9 (-1237)*T/ C were determined by polymerase chain reaction. It was found that, among the COVID-19 patients, a TLR-9 allele (-1237 C) with higher transcriptional activity was more common than in the control group (19.421% and 11.275%, respectively, p = 0.019), and its homozygous genotype TLR-9 (-1237)*C was not detected in the comparison group. TLR-9 allele (-1237)*T in the patients with COVID-19 was less common in comparison with the control group (80.579% and 88.725%, respectively, p = 0.019). Taking into account the differences in suggested TLR-9 expression in more severe COVID-19 patients, we compared distribution of TLR-9 (-1237)*T/ С allele polymorphism in the patients with different severity of COVID-19. In the group of patients with mild form, the TLR-9 (-1237)*T/T genotype was more common as compared with patients who had more severe clinical course. The differences were significant at the trend level when compared with patients with a medium-severity disease (86.364% and 66,000%, respectively; p = 0.076).
{"title":"TLR-9 (-1237)*T/C polymorphism in russian COVID-19 patients from the chelyabinsk region","authors":"S. Belyaeva, T. Suslova, Daria C. Stashkevich, Svetlana E. Balandina, Daria E. Mjakotina, Maria S. Milonchenko","doi":"10.46235/1028-7221-9904-tpi","DOIUrl":"https://doi.org/10.46235/1028-7221-9904-tpi","url":null,"abstract":"In COVID-19, the clinical outcome depends on a wide range of factors, including genetic features. Among them, TLRs, the genes encoding the receptors of innate immune system are of particular interest since they play the key role in development of innate immune response. The present study concerns the newely identified allelic variants of the TLR-9 (-1237)*T/C gene in Russian residents from the Chelyabinsk Region who had COVID-19 complicated by the bilateral viral pneumonia. Polymorphic variants of TLR-9 (-1237)*T/ C were determined by polymerase chain reaction. It was found that, among the COVID-19 patients, a TLR-9 allele (-1237 C) with higher transcriptional activity was more common than in the control group (19.421% and 11.275%, respectively, p = 0.019), and its homozygous genotype TLR-9 (-1237)*C was not detected in the comparison group. TLR-9 allele (-1237)*T in the patients with COVID-19 was less common in comparison with the control group (80.579% and 88.725%, respectively, p = 0.019). Taking into account the differences in suggested TLR-9 expression in more severe COVID-19 patients, we compared distribution of TLR-9 (-1237)*T/ С allele polymorphism in the patients with different severity of COVID-19. In the group of patients with mild form, the TLR-9 (-1237)*T/T genotype was more common as compared with patients who had more severe clinical course. The differences were significant at the trend level when compared with patients with a medium-severity disease (86.364% and 66,000%, respectively; p = 0.076).","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73265705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-9970-eoc
E. Pashkina, A. Aktanova, E. Kovalenko, V. Kozlov
Immunological safety of nanoparticles is an urgent problem for development of drug delivery systems used as the basis for creating these systems. Cucurbit[n]urils (CB[n]) are molecular nanocontainers that can encapsulate various drugs and serve as the basis for delivery systems. Cucurbiturils are low-toxic compounds: under in vitro conditions, they exhibit weak immunomodulatory properties, without sufficient immunotoxicity. The aim of the present study was to evaluate the in vivo effects of cucurbiturils on the number and differential leukocyte counts in peripheral blood. BALB/c mice aged 2-4 months were used in the work. For this study, cucurbiturils were diluted in phosphate-buffered saline and administered to laboratory animals intraperitoneally (three times a week). When evaluating the effect of cucurbit[7]uril on blood parameters of the animals after three intraperitoneal injections weekly, no statistically significant changes were registered. However, at first administration, the animals showed a slight increase in relative number of lymphocytes after the first injection of CB[6], and an increased proportion of neutrophils after the first administration of CB[8]. Meanwhile, the proportions of lymphocytes and neutrophils were within normal ranges after the 2nd and 3rd injections of cucurbiturils, and did not show any significant differences against the controls. Moreover, the impact upon the subpopulation composition of peripheral blood lymphocytes in laboratory animals was assessed. After injection of CB[6], there was a decrease in T lymphocytes in the peripheral blood, along with increased proportion of CD19+B lymphocytes compared with the controls. CB[7] and CB[8] injections did not affect the subpopulation profile of peripheral blood lymphocytes. We have found that intraperitoneal administration of CB[n] did not affect the blood parameters of laboratory animals, thus, probably suggesting the in vivo safety of these compounds. At the same time, CB[6] is able to exert a stimulating effect on humoral immunity by increasing relative contents of B lymphocytes.
{"title":"Effect of cucurbiturils on the numbers and differential counts of peripheral blood leukocytes in laboratory animals after in vivo parenteral administration","authors":"E. Pashkina, A. Aktanova, E. Kovalenko, V. Kozlov","doi":"10.46235/1028-7221-9970-eoc","DOIUrl":"https://doi.org/10.46235/1028-7221-9970-eoc","url":null,"abstract":"Immunological safety of nanoparticles is an urgent problem for development of drug delivery systems used as the basis for creating these systems. Cucurbit[n]urils (CB[n]) are molecular nanocontainers that can encapsulate various drugs and serve as the basis for delivery systems. Cucurbiturils are low-toxic compounds: under in vitro conditions, they exhibit weak immunomodulatory properties, without sufficient immunotoxicity. The aim of the present study was to evaluate the in vivo effects of cucurbiturils on the number and differential leukocyte counts in peripheral blood. \u0000BALB/c mice aged 2-4 months were used in the work. For this study, cucurbiturils were diluted in phosphate-buffered saline and administered to laboratory animals intraperitoneally (three times a week). \u0000When evaluating the effect of cucurbit[7]uril on blood parameters of the animals after three intraperitoneal injections weekly, no statistically significant changes were registered. However, at first administration, the animals showed a slight increase in relative number of lymphocytes after the first injection of CB[6], and an increased proportion of neutrophils after the first administration of CB[8]. Meanwhile, the proportions of lymphocytes and neutrophils were within normal ranges after the 2nd and 3rd injections of cucurbiturils, and did not show any significant differences against the controls. Moreover, the impact upon the subpopulation composition of peripheral blood lymphocytes in laboratory animals was assessed. After injection of CB[6], there was a decrease in T lymphocytes in the peripheral blood, along with increased proportion of CD19+B lymphocytes compared with the controls. CB[7] and CB[8] injections did not affect the subpopulation profile of peripheral blood lymphocytes. \u0000We have found that intraperitoneal administration of CB[n] did not affect the blood parameters of laboratory animals, thus, probably suggesting the in vivo safety of these compounds. At the same time, CB[6] is able to exert a stimulating effect on humoral immunity by increasing relative contents of B lymphocytes.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87472690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-9637-lot
N. S. Chepurnova, S. V. Knysh, A. Rudneva, Viktoria V. Zdor, E. Markelova, Polina V. Romanjuk, Semyon I. Sayapin
Multiple studies are currently, aimed at studying the mechanisms of the development of basic age-related pathologies, e.g., arterial hypertension (AH). We found no literature sources concerning associations between the levels of matrix metalloproteinase type 7 (MMP-7), tissue inhibitor of matrix metalloproteinases type (TIMP-1) and anthropometric data which could be useful for diagnosing age-associated, socially dependent diseases and metabolic disorders. The article presents the data on the state of proteolysis system and anthropometric data of 45 almost healthy women and 45 women with arterial hypertension (AH) aged from 59 to 74 years old. The levels of MMP-7 and TIMP-1 in blood serum were studied by sandwich-variant of ELISA test. The waist and neck volume were measured three times with a centimeter tape at an accuracy of 1 cm. Statistical processing of the obtained data was performed using analytical software IBM SPSS Statistics, v. 22.0. The results show some correlations between the levels of MMP-7 and TIMP-1, and the waist and neck sizes (WC). Lower levels of MMP-7 and TIMP-1 were registered in the group of practically healthy women as compared to the second (AH) study group (p 0.01). Anthropometric data were not markedly diverse: the waist size in the group of healthy women was less than in AH group (p 0.05), and values of neck volume did not differ significantly between the groups. When assessing the relationships in the group of practically healthy women, we recorded a direct significant correlation between the waist size and neck volume (r = 0.754, p 0.0001), while in the group of women with AH this relationship was weaker (r = 0.5782, p 0.0001). In addition, a similar correlation was found between MMP-7 and TIMP-1 levels in the both groups studied. In the group of healthy women this relationship was of medium strength (r = 0.657, p 0.0001), whereas in the group of women with AH it was stronger (r = 0.720, p 0.0001). The data obtained suggest the presence of metabolic disorders in the women with arterial hypertension and signs of proteolytic enzyme system disorders.
目前有多项研究旨在研究与年龄相关的基本病理，如动脉高血压(AH)的发展机制。我们没有发现关于基质金属蛋白酶7型(MMP-7)、基质金属蛋白酶组织抑制剂(TIMP-1)水平与人体测量数据之间关系的文献来源，这些数据可用于诊断年龄相关、社会依赖疾病和代谢紊乱。本文报道了45例59 ~ 74岁几乎健康妇女和45例高血压妇女的蛋白水解系统状态和人体测量资料。采用夹心式ELISA法检测血清中MMP-7和TIMP-1的水平。腰颈体积用厘米卷尺测量三次，精确度为1厘米。采用IBM SPSS Statistics, v. 22.0分析软件对所得数据进行统计处理。结果表明，MMP-7和TIMP-1水平与腰颈尺寸(WC)存在一定的相关性。与第二研究组(AH)相比，实际健康妇女组的MMP-7和TIMP-1水平较低(p 0.01)。人体测量数据无明显差异:健康妇女组腰围小于AH组(p < 0.05)，两组间颈部体积值无显著差异。在评估实际健康女性组的关系时，我们记录了腰围大小和颈部体积之间的直接显著相关性(r = 0.754, p 0.0001)，而在AH女性组中，这种关系较弱(r = 0.5782, p 0.0001)。此外，在两组研究中发现MMP-7和TIMP-1水平之间存在类似的相关性。在健康妇女组中，这种关系为中等强度(r = 0.657, p 0.0001)，而在AH妇女组中，这种关系更强(r = 0.720, p 0.0001)。获得的数据表明，存在代谢障碍的妇女动脉高血压和蛋白水解酶系统紊乱的迹象。
{"title":"Levels of tissue inhibitor of matrix metalloproteinases type 1, matrix metalloproteinase type 7 and anthropometric parameters in practically healthy women and women with arterial hypertension in the older age group","authors":"N. S. Chepurnova, S. V. Knysh, A. Rudneva, Viktoria V. Zdor, E. Markelova, Polina V. Romanjuk, Semyon I. Sayapin","doi":"10.46235/1028-7221-9637-lot","DOIUrl":"https://doi.org/10.46235/1028-7221-9637-lot","url":null,"abstract":"Multiple studies are currently, aimed at studying the mechanisms of the development of basic age-related pathologies, e.g., arterial hypertension (AH). We found no literature sources concerning associations between the levels of matrix metalloproteinase type 7 (MMP-7), tissue inhibitor of matrix metalloproteinases type (TIMP-1) and anthropometric data which could be useful for diagnosing age-associated, socially dependent diseases and metabolic disorders. The article presents the data on the state of proteolysis system and anthropometric data of 45 almost healthy women and 45 women with arterial hypertension (AH) aged from 59 to 74 years old. The levels of MMP-7 and TIMP-1 in blood serum were studied by sandwich-variant of ELISA test. The waist and neck volume were measured three times with a centimeter tape at an accuracy of 1 cm. Statistical processing of the obtained data was performed using analytical software IBM SPSS Statistics, v. 22.0. The results show some correlations between the levels of MMP-7 and TIMP-1, and the waist and neck sizes (WC). Lower levels of MMP-7 and TIMP-1 were registered in the group of practically healthy women as compared to the second (AH) study group (p 0.01). Anthropometric data were not markedly diverse: the waist size in the group of healthy women was less than in AH group (p 0.05), and values of neck volume did not differ significantly between the groups. When assessing the relationships in the group of practically healthy women, we recorded a direct significant correlation between the waist size and neck volume (r = 0.754, p 0.0001), while in the group of women with AH this relationship was weaker (r = 0.5782, p 0.0001). In addition, a similar correlation was found between MMP-7 and TIMP-1 levels in the both groups studied. In the group of healthy women this relationship was of medium strength (r = 0.657, p 0.0001), whereas in the group of women with AH it was stronger (r = 0.720, p 0.0001). The data obtained suggest the presence of metabolic disorders in the women with arterial hypertension and signs of proteolytic enzyme system disorders.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91203246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-7738-cpi
A. Korkmazov, E. O. Kiseleva, Violetta D. Frolova, G.D. Romanyugo
A brief review of studies on one of the topical issues of otorhinolaryngology - polypous rhinosinusitis in people with recurrent infections is given.It was noted that patients with polyposis rhinosinusitis are more often committed to acute respiratory infections than those without nasal polyposis and they have a significant immunological imbalance.Serum levels of IL-8, IL-1, anti-inflammatory IL-4 and IFN- production were examined in 48 frequently ill patients with a history of respiratory allergopathology and polypous sinusitis.All patients, according to clinical recommendations, received complex conservative therapy.The control group consisted of 21 people who had respiratory infections no more than one or two times during the year and a history that was not aggravated by polypous rhinosinusitis.As a result, a significant decrease in IFN- levels and an increase in the concentrations of IL-8, IL-1, and IL-4 were revealed, which in turn confirms a violation of the body's immunoreactivity, a decrease in local and systemic immunity in patients with recurrent infections and polypous rhinosinusitis.The results obtained predetermine the need for the use of immunocorrective measures in the complex therapy of these patients.
{"title":"Cytokine profile in adolescent children with recurrent infections and polypous rhinosinusitis, ways of their correction","authors":"A. Korkmazov, E. O. Kiseleva, Violetta D. Frolova, G.D. Romanyugo","doi":"10.46235/1028-7221-7738-cpi","DOIUrl":"https://doi.org/10.46235/1028-7221-7738-cpi","url":null,"abstract":"A brief review of studies on one of the topical issues of otorhinolaryngology - polypous rhinosinusitis in people with recurrent infections is given.It was noted that patients with polyposis rhinosinusitis are more often committed to acute respiratory infections than those without nasal polyposis and they have a significant immunological imbalance.Serum levels of IL-8, IL-1, anti-inflammatory IL-4 and IFN- production were examined in 48 frequently ill patients with a history of respiratory allergopathology and polypous sinusitis.All patients, according to clinical recommendations, received complex conservative therapy.The control group consisted of 21 people who had respiratory infections no more than one or two times during the year and a history that was not aggravated by polypous rhinosinusitis.As a result, a significant decrease in IFN- levels and an increase in the concentrations of IL-8, IL-1, and IL-4 were revealed, which in turn confirms a violation of the body's immunoreactivity, a decrease in local and systemic immunity in patients with recurrent infections and polypous rhinosinusitis.The results obtained predetermine the need for the use of immunocorrective measures in the complex therapy of these patients.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75513855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-9635-eog
D. Usanina, S. Uzhviyuk, S. Zamorina
Graphene and its derivatives are materials with unique physicochemical properties. A detailed study of these materials allows to consider them prospective biomedical agents for targeted drug and gene delivery, photothermal therapy of cancer, bioimaging, etc. However, this requires a comprehensive studies of their effects on the body tissues, including cells of the immune system. The aim of our research was to stydy the effects of nanoparticles based on pegylated graphene oxide (GO) upon apoptosis of T lymphocytes derived from blood of healthy donors and Jurkat 5332 cell line. Comparison of these cells will extend our knowledge of the effects of nanomaterials on the cells, and to respond the question, what results obtained with continuous cell lines are valid for normal non-malignant cells. In this work, we used GO nanoparticles (100-200 nm, 1-5 m) coated with linear (LP-GO) and branched (BP-GO) polyethylene glycol (PEG). The cells were cultured for 24 hours at 37 C and 5% CO2with nanoparticles at concentrations of 5 and 25 g/mL. Viability and early and late apoptosis of incubated Jurkat cells and CD3+cells from healthy donors were assessed by flow cytometry. It was found that the small nanoparticles coated with linear PEG at high concentrations (25 g/mL) could significantly reduce the number of live cells and increase the number of cells in late apoptosis. At the same time, large nanoparticles coated with branched PEG at high concentrations (25 g/mL) increased the percentage of T cells in early apoptosis. Meanwhile, the GO nanoparticles at both concentrations did not affect the viability and apoptosis of Jurkat cells, regardless of the size, concentration, and type of surface function of the particles. The obtained results suggest that GO nanoparticles exert different effects upon normal and malignant lymphocytes of T lineage. One may assume that these discrepancies could be explained by greater resistance of tumor cells compared to normal T cells. These findings suggests that studies of nanomaterials upon living cells should not be limited to experiments on cell lines, since their properties may significantly differ from those of non-malignant cells.
{"title":"Effect of graphene oxide nanoparticles on apoptosis of T-lymphocytes and Jurkat cells","authors":"D. Usanina, S. Uzhviyuk, S. Zamorina","doi":"10.46235/1028-7221-9635-eog","DOIUrl":"https://doi.org/10.46235/1028-7221-9635-eog","url":null,"abstract":"Graphene and its derivatives are materials with unique physicochemical properties. A detailed study of these materials allows to consider them prospective biomedical agents for targeted drug and gene delivery, photothermal therapy of cancer, bioimaging, etc. However, this requires a comprehensive studies of their effects on the body tissues, including cells of the immune system. \u0000The aim of our research was to stydy the effects of nanoparticles based on pegylated graphene oxide (GO) upon apoptosis of T lymphocytes derived from blood of healthy donors and Jurkat 5332 cell line. Comparison of these cells will extend our knowledge of the effects of nanomaterials on the cells, and to respond the question, what results obtained with continuous cell lines are valid for normal non-malignant cells. In this work, we used GO nanoparticles (100-200 nm, 1-5 m) coated with linear (LP-GO) and branched (BP-GO) polyethylene glycol (PEG). The cells were cultured for 24 hours at 37 C and 5% CO2with nanoparticles at concentrations of 5 and 25 g/mL. Viability and early and late apoptosis of incubated Jurkat cells and CD3+cells from healthy donors were assessed by flow cytometry. It was found that the small nanoparticles coated with linear PEG at high concentrations (25 g/mL) could significantly reduce the number of live cells and increase the number of cells in late apoptosis. At the same time, large nanoparticles coated with branched PEG at high concentrations (25 g/mL) increased the percentage of T cells in early apoptosis. \u0000Meanwhile, the GO nanoparticles at both concentrations did not affect the viability and apoptosis of Jurkat cells, regardless of the size, concentration, and type of surface function of the particles. \u0000The obtained results suggest that GO nanoparticles exert different effects upon normal and malignant lymphocytes of T lineage. One may assume that these discrepancies could be explained by greater resistance of tumor cells compared to normal T cells. These findings suggests that studies of nanomaterials upon living cells should not be limited to experiments on cell lines, since their properties may significantly differ from those of non-malignant cells.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85579345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-9961-sod
A. A. Zhuzhula, O. Kurbatova, M. Snovskaya, S. Petrichuk, T. Komyagina, Anastasia S. Tryapochkina
Systemic connective tissue diseases (SCTD) are characterized by systemic autoimmune inflammation and are accompanied by development of antinuclear antibodies (ANA). Our aim was a comparative analysis of ANA in blood serum in children with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). The study included 50 patients with SLE, 50 patients with RA who were treated at the National Medical Research Center of Childrens Health. Median age was 12,2 y. o. (9-15.5). The titers of ANA and the cell fluorescence type were determined with the indirect immunofluorescence reaction (IFR) using the HEp-2 cell line (Immco Diagnostics, Inc, USA), as well as the concentration of antibodies to double-stranded DNA (adsDNA) in blood serum samples of the children detected by immunochemiluminescence (ICM) woth Elia dsDNA (Thermo Fisher Scientific, USA). A positive ANA titer and adsDNA were found, respectively, in 98% and in 48% in children with SLE. A positive ANA titer and adsDNA was detected in 100% and in 4% of children with RA, respectively. Highly positive ANA titers ( 1/1280) have been detected in 68% of children with SLE, and in 30% of children with RA. None of the RA patients with highly positive ANA titers had adsDNA. But, in patients with SLE, highly positive ANA titers and a positive dsDNA level were simultaneously detected in 16% of cases. There are both single types of cell fluorescence and their combinations in children with SLE and RA. Nuclear dot-like fluorescence was more common in children with SLE, cytoplasmic type, in children with RA, nucleolar type of glow was found only in children with RA. The revealed combinations of ANA and adsDNA titers in children with SLE and RA confirm the need for simultaneous use of RNIF and ICM.
{"title":"Significane of determining antinuclear antibodies in systemic connective tissue disorders in children","authors":"A. A. Zhuzhula, O. Kurbatova, M. Snovskaya, S. Petrichuk, T. Komyagina, Anastasia S. Tryapochkina","doi":"10.46235/1028-7221-9961-sod","DOIUrl":"https://doi.org/10.46235/1028-7221-9961-sod","url":null,"abstract":"Systemic connective tissue diseases (SCTD) are characterized by systemic autoimmune inflammation and are accompanied by development of antinuclear antibodies (ANA). Our aim was a comparative analysis of ANA in blood serum in children with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). The study included 50 patients with SLE, 50 patients with RA who were treated at the National Medical Research Center of Childrens Health. Median age was 12,2 y. o. (9-15.5). The titers of ANA and the cell fluorescence type were determined with the indirect immunofluorescence reaction (IFR) using the HEp-2 cell line (Immco Diagnostics, Inc, USA), as well as the concentration of antibodies to double-stranded DNA (adsDNA) in blood serum samples of the children detected by immunochemiluminescence (ICM) woth Elia dsDNA (Thermo Fisher Scientific, USA). A positive ANA titer and adsDNA were found, respectively, in 98% and in 48% in children with SLE. A positive ANA titer and adsDNA was detected in 100% and in 4% of children with RA, respectively. Highly positive ANA titers ( 1/1280) have been detected in 68% of children with SLE, and in 30% of children with RA. None of the RA patients with highly positive ANA titers had adsDNA. But, in patients with SLE, highly positive ANA titers and a positive dsDNA level were simultaneously detected in 16% of cases. There are both single types of cell fluorescence and their combinations in children with SLE and RA. Nuclear dot-like fluorescence was more common in children with SLE, cytoplasmic type, in children with RA, nucleolar type of glow was found only in children with RA. The revealed combinations of ANA and adsDNA titers in children with SLE and RA confirm the need for simultaneous use of RNIF and ICM.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90017993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.46235/1028-7221-10013-rom
O. Kurbatova, S. Petrichuk, G. Movsisyan, Daria G. Kuptsova, T. Radygina, A. Anushenko, E. Semikina, A. Potapov
Glycogen storage disease (GSD) is a rare condition that changes the way the body uses and stores glycogen. Objective: to evaluate the content of small populations of lymphocytes and their ratios in children with hepatic forms of glycogen disease depending on the stage of liver fibrosis. 148 children with GSD at the age of Me=7,7 [3,9;11,8] were examined. The comparison group consisted of 54 healthy children. The stage of liver fibrosis was carried out on the FibroScan F502 device (EchoSence, France). Immunophenotyping of lymphocytes was performed on CYTOMICS FC500 (Beckman Coulter, USA). Indicators of lymphocyte populations were analyzed as a percentage of deviation from the age norm. In children with GSD, an increase in the degree of liver fibrosis was revealed from age (R=0.57). Treg content in children with GSD was at the lower limit of the age norm and did not depend on the stage of liver fibrosis. The content of Th17 and Thact lymphocytes was significantly higher than that of the comparison group at all stages of liver fibrosis, starting from stage F1. With an increase in the stage of liver fibrosis, there was an increase in the proportion of patients with Thact and Th17 content exceeding the upper limit of the normative values (PF0-F4=0.021 and PF0-F4=0.012, respectively). An increase in Th17/Treg and Thact/Treg ratios was revealed in patients with GSD relative to the comparison group in all age groups, the dynamics of Th17/Treg and Thact/Treg ratios was characterized by their increase with age. Analysis of indicators depending on the stage of liver fibrosis in children with GSD revealed a significant increase in the Thact/Treg ratio from stage F0 to stages F1, F2, F3 and F4 (PF0-F4=0.000). The Th17/Treg index increased from stage F0 to stages F1, F2, F3 (PF0-F3=0.000). An increase in the content of Thact and Th17 lymphocytes, as well as Th17/Treg and Thact/Treg indices with an increase in the stage of liver fibrosis can be used as an additional tool in assessing fibrotic changes in the liver. Immunological indicators objectively reflect the severity of the patient's condition with hepatic forms of GSD.
{"title":"Role of minor lymphocyte populations in development of liver fibrosis in children with glycogen storage disease","authors":"O. Kurbatova, S. Petrichuk, G. Movsisyan, Daria G. Kuptsova, T. Radygina, A. Anushenko, E. Semikina, A. Potapov","doi":"10.46235/1028-7221-10013-rom","DOIUrl":"https://doi.org/10.46235/1028-7221-10013-rom","url":null,"abstract":"Glycogen storage disease (GSD) is a rare condition that changes the way the body uses and stores glycogen. Objective: to evaluate the content of small populations of lymphocytes and their ratios in children with hepatic forms of glycogen disease depending on the stage of liver fibrosis. 148 children with GSD at the age of Me=7,7 [3,9;11,8] were examined. The comparison group consisted of 54 healthy children. The stage of liver fibrosis was carried out on the FibroScan F502 device (EchoSence, France). Immunophenotyping of lymphocytes was performed on CYTOMICS FC500 (Beckman Coulter, USA). Indicators of lymphocyte populations were analyzed as a percentage of deviation from the age norm. In children with GSD, an increase in the degree of liver fibrosis was revealed from age (R=0.57). Treg content in children with GSD was at the lower limit of the age norm and did not depend on the stage of liver fibrosis. The content of Th17 and Thact lymphocytes was significantly higher than that of the comparison group at all stages of liver fibrosis, starting from stage F1. With an increase in the stage of liver fibrosis, there was an increase in the proportion of patients with Thact and Th17 content exceeding the upper limit of the normative values (PF0-F4=0.021 and PF0-F4=0.012, respectively). An increase in Th17/Treg and Thact/Treg ratios was revealed in patients with GSD relative to the comparison group in all age groups, the dynamics of Th17/Treg and Thact/Treg ratios was characterized by their increase with age. Analysis of indicators depending on the stage of liver fibrosis in children with GSD revealed a significant increase in the Thact/Treg ratio from stage F0 to stages F1, F2, F3 and F4 (PF0-F4=0.000). The Th17/Treg index increased from stage F0 to stages F1, F2, F3 (PF0-F3=0.000). \u0000An increase in the content of Thact and Th17 lymphocytes, as well as Th17/Treg and Thact/Treg indices with an increase in the stage of liver fibrosis can be used as an additional tool in assessing fibrotic changes in the liver. Immunological indicators objectively reflect the severity of the patient's condition with hepatic forms of GSD.","PeriodicalId":21524,"journal":{"name":"Russian Journal of Immunology","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89880952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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