Saudi Journal of Biological Sciences最新文献

Background

Multiple sclerosis (MS) is a neurodegenerative disease characterized by inflammation and demyelination of neurons. There is evidence to suggest that level of a neurotransmitter gamma-aminobutyric acid (GABA), due to the degradation by γ-aminobutyric acid transaminase (GABAT), is reduced in certain areas of the brain in MS patients. MS is always accompanied by gut bacteria dysbiosis. In healthy individuals, Faecalibacterium sp. while in MS patients A. calcoaceticus, Clostridium sp. and S. typhimurium are found abundantly. Although all these microbes produce GABAT but only in MS patients this enzyme significantly degrades GABA.

Objective

Present study is an attempt to characterize the GABAT protein sequences of these bacteria.

Methodology

Sequences of GABAT protein were retrieved from Uniprot database. Sequences were analyzed by Protparam, Gneg-mPLoc, SOSUI, PFP-FunDSeqE, Pepwheel program, PROTEUS and Alphafold and SAVES servers, MEME suite and HDOCK server.

Results

In healthy individuals gastrointestinal tract (GIT) bacteria, GABAT protein was present in inner-membrane with α helix content (61 and 62%) and β sheet content (5%), 4-helical cytokines functional domains. It has greater number of B-cell epitopes and more complex 3D configuration as compared to MS patients GIT bacterial enzymes.

Conclusion

Present study might enable us to modify the GABAT encoding gene and enzyme through site-directed mutagenesis in pathogenic bacteria thus reducing their potential of causing MS.

The pH1N1 belongs to influenza A family that is sometimes transmitted to humans via contact with pigs. Human tonsillar immune cells are widely used as in vitro models to study responses to influenza viruses. In the current study, human memory (M) and naïve (N) T cells responses in mononuclear cells of tonsil (TMCs) and peripheral blood (PBMCs) were stimulated by pH1N1/sH1N1, and then stained for estimation of T cells proliferation index. Individuals with an anti-pH1N1 hemagglutination (HA) inhibition (HAI) titer of forty or greater exhibited stronger HA-specific M-CD4⁺ T cells responses to pH1N1 in TMCs/PBMCs than those with an HAI titer of less than forty (P < 0.01). In addition, a positive correlation was observed between proliferation indices of M-CD4⁺ T cells induced by exposure to sH1N1/pH1N1 (p < 0.01). Moreover, a strong correlation (p < 0.001) was detected between subjects’ age and their HA-specific M-CD4⁺ T cells induced by pH1N1 exposure, indicating that this response was age-dependent. Finally, stimulation of TMCs with pH1N1-HA resulted in a significant M−CD8⁺ T cells response (p < 0.05). In conclusion, pH1N1 HA elicits a strong M-CD4⁺ T cells response in TMCs. Additionally, this response correlates with the response to sH1N1 suggesting cross-reactivity in T cells epitopes directed against HAs of both viral strains.

A species introduced outside of its native range will likely encounter unusual abiotic and biotic conditions,and may exhibit phenotypic traits that may facilitate survival and persistance. Phenotypic plasticity drives non-native species' development of adaptive traits in the new environment, increases their fitness, and as a result, contributes to invasion success. In this study, we examined inter and intraspecific phenotypic variation (body size and shape) for an invasive (Carassius gibelio) and introduced (Cyprinus carpio) cyprinid fish species (Teleostei: Cyprinidae) in the Düden Stream, Turkey, which is a small-scale river system. We hypothesized that interspecific phenotypic variation correlates with fish-specific variables and river site. We further hypothesized that these two species may exhibit similar phenotypic variation patterns between populations. The MANCOVA revealed that species-specific traits, river site, had significant effects on body shape variation and size along the stream. The differences in the shape of the head, the central portion of the body, and fins in both species most probably reflected differences in the swimming and feeding of the fish, possibly to avoid interspecies competition. The intraspecific phenotypic variation observed in both species may indicate rapid local adaptation, triggered by multiple founding event, or/and phenotypic plasticity.

The geranium genus consists of about 400 species, which have been utilized for a long time in ancient medical practices throughout the world. As a result, herbal medications based on species are commonly utilized to treat a range of illnesses. This investigation aims to provide an extensive assessment of the literature on the phytochemistry, ethnomedicinal and pharmacological importance of the genus Geranium. Data were collected through systemic computer searches among the most reputable scientific databases, Web of Science, Google Scholar, and Scopus. Occasionally, information published as peer-reviewed literature was added to data from sources that these databases do not include. This review includes all published works through the end of 2022. The assessment of the biological characteristics of medicinal plant species in the genus Geranium has received a great deal of attention, primarily in the last 20 years, in tandem with the growing interest in herbal remedies in general. The detailed and systematic comparative analysis presented here provides valuable information on the current Geranium species. It paves the way for other beneficial species of Geranium to be studied in the fields of ethnobotany, phytochemistry, and new drug discovery.

In the current study the assessment of the antimicrobial and phytochemical properties of Cassia fistula, Musa paradisiaca, Ficus religiosa and Murraya koenigii plants extracts was carried out. The antibacterial potential of these plants extracts was tested against S. aureus and E. coli. The Cassia fistula and Ficus religiosa leaves showed the larger zone of inhibition in aqueous and butanolic extract respectively against Escherichia coli. Musa paradisiaca and Murraya koenigii leaves showed larger zone of inhibition in ethanolic extract against S. aureus. Qualitative phytochemical analysis showed the presence of alkaloids, flavonoids, phenols, terpenoids, steroids, glycosides, saponins, carbohydrates, proteins and tannins in all extracts while phylobatannins, emodins, anthocyanins and leucoanthocyanins were not present in these extracts. Quantitative phytochemical analysis showed the highest alkaloid content in the Murraya koenigii leaves. Highest tannin content and flavonoid content was found in Ficus religiosa leaves, while highest phenolic content was found in case of Cassia fistula. In addition to this antioxidant potential of all the extracts was determined. Musa paradisiaca leaves showed highest antioxidant potential as compared to other plant extracts. In silico analysis of bioactive components present in plant extracts was performed by molecular docking. The rutin and Glu from Musa paradisiaca and Murraya koenigii respectively, were docked with Glycogen Synthase Kinase 3 beta (1GSK-3beta) protein. Quercetin and rutin from Cassia fistula and Ficus religiosa respectively, were docked with C- reactive protein (CRP). The tested bioactive compounds showed good binding affinity with significant number of hydrogen bonds and can be used as a good alternative of synthetic drugs to treat rheumatism and wounds.

The present study was aimed at evaluating the genetic variation and population structure in a collection of 22 rice genotypes. Twenty-two rice genotypes were assessed using quantitative traits and SSR molecular markers for genetic variability and genetic diversity. As for genetic diversity, the genotypes were clarified based on twelve quantitative traits. Clustering produced two large groups: the IR70423-169-2-2 variety was in a branch alone due to its long duration, while, the second group included all rest of genotypes and was split up into two sub-groups. The first sub-group included IR67418-131-2-3-3-3, IR67420-206-3-1-3-3, Giza181, Giza182, Sakha104, and P1044-86-5-3-3-2M. However, pedigree played in divided clustering with Giza181 and Giza182, which were belonging to the Indica type and produced from the same parents. SSR markers produced 87 alleles, with a mean of 4.3 alleles per locus, which were detected in 22 rice genotypes. A higher number of alleles were found with primers RM262, RM244, RM3843, RM212, and RM3330. With an overall mean of 0.837, the polymorphic information content values were high for all SSR markers, ranging from a low of 0.397 for M254 to a high of 0.837 for RM244. The dendogram was divided into six groups according to the types of genotypes, with the pedigree playing a major role for the genetic distance. In order to help breeders choose parents and create suitable hybrids to achieve genetic improvement in crops, particularly rice, SSR is a useful technique for analysing genotype diversity and aiding in the genetic fingerprinting of each variety.

The current research endeavor seeks to unlock the potential of orally administered insulin formulations by utilizing liposomes derived from the fat globule membrane (MFGM) of camel milk as carriers for insulin. This pursuit emerges as a result of the recognized limitations of subcutaneous insulin therapy. The liposomes were meticulously created using the thin film hydration method, followed by comprehensive chemical and morphological analyses. Additionally, comprehensive safety assessments were carried out in vitro and in vivo, revealing significant findings.

The Fourier-transform infrared (FTIR) spectrum confirmed the presence of insulin within the liposomes, demonstrating changes in their size and charge. The in vitro cytotoxicity analysis, performed on HEK-293 cell lines through the MTT assay, yielded results indicating a cell viability of over 90%. In the in vivo investigation, diabetic rats induced by STZ were utilized to evaluate the effects of the liposomes, revealing substantial reductions in blood glucose levels, bilirubin, alkaline phosphatase (ALP), albumin, and alanine aminotransferase (ALT) levels. Hepatic histopathological assessments showed signs of recovery across all treatment groups, with no observable microscopic changes in renal tissue. This investigation highlights the significant hypoglycemic effects observed in insulin-loaded liposomes derived from MFGM obtained from camel milk when administered orally.

In response to growing concerns about the efficacy of antibiotic treatment, there has been a significant increase in research on bacteria that are resistant to antibiotics over the past two centuries. Such investigations might bring a spotlight on the field's evolution and future prospects. The study was aimed at conducting a measurable bibliometric review of the scientific literature on bacterial persistence and dormancy in the 21st Century. A scientific literature published during 21st Century was analyzed to gain insights into and identify research trends and outputs in persistent bacteria. Bibliometrix (R language package) and the VOS viewer were used to conduct a bibliometric investigation to determine the globally indexed persistent bacteria research output. WoS Core Collection databases were searched for persistent bacteria selected as the subject. A total of 1,160 published documents from 495 sources from the preceding two decades were reviewed. Maximum publications of 112 were observed in 2021 with 860 citations; however, 82 publications appeared in 2015 and were able to get the highest number of citations (4,214), only 43 (3.7%) were single-authored, whereas 1,117 (96.3%) publications are the result of collaborative works. Out of the top 10 countries ranked for publications, the USA took the top spot for the most highly productive country with 435 articles. Dormancy’ appeared 2,351 times, followed by ‘Escherichia coli“ (1,744, and ‘Growth’ 1,184 times) in research publications on bacterial persistence research. The findings from this study will aid in the creation of strategies and guidelines for regulating and avoiding bacterial persistence status.

Shrimp aquaculture has rapidly developed into a significant industry worldwide, providing not only financial gain and high-quality food but also tens of thousands of trained and competent workers. Frequent diseases are now regarded as a significant risk factor for shrimp aquaculture, as they have the potential to significantly reduce shrimp production and result in economic losses. Over the years various traditional methods including the use of antibiotics have been followed to control diseases yet unsuccessful. Probiotic is considered potential supplements for shrimps during farming, they may also act beneficially as disease control and increased production. Probiotics are described as a live microbial supplement that benefits the host by modifying the microbial population associated with the host and its ambient. The present state of research about probiotics demonstrates notable impacts on the immune defences of the host's gastrointestinal system, which play a crucial role in safeguarding against diseases and managing inflammation inside the digestive tract. In the past ten years, many studies on probiotics have been published. However, there is a lack of information about the processes by which probiotics exert their effects in aquaculture systems, with only limited elucidations being offered. This study explores the variety of procedures behind the positive effects of probiotics in shrimp culture. These mechanisms include the augmentation of the immune system, control of growth, antagonistic action against pathogens, competitive exclusion, and modification of the gut microbiota. Mechanisms involved in the probiotic mode of action are mostly interlinked. This provides a greater understanding of the importance of probiotics in shrimp culture as an environmentally friendly practice.