Saudi Journal of Biological Sciences最新文献

Background

To increase crop productivity, modern agricultural practices comprises fertilizers, algaecides, herbicides and fungicides.

Objective

The purpose of this study was to evaluate the effects of soil microbial population and soil enzyme activity by the use of fertilizer in maize and inorganic input in the rice ecosystem.

Methods

A field experiment (2021 to 2023) was carried out using synthetic fertilizer doses with maize crops followed by rice crops using inorganic inputs. Soil microbial population and enzyme activities were examined.

Results

Maize field experiment revealed that the plots treated with 75 % Standardized Dose of Fertilizer (SDF) of NPK had the highest populations of diazotrophs (124 × 10⁵cfu / g), Phosphobacteria (66.33 × 10⁵cfu / g), and Azospirillum (0.409 × 10⁵ MPN / g) than 100 % and 150 % SDF of NPK. The soil enzyme activity was higher in the unfertilized control plot than fertilized plot. These experimental results revealed that a low amount of fertilizer and no fertilizers favour the growth of soil microorganisms and soil enzyme activities, respectively. Followed by the rice field experiment, revealed that the soil microbial population was decreased by the application of inorganic inputs viz., fertilizer, algaecide, herbicide and fungicide. However, the maximum soil microbial population was found in algaecide application followed by herbicide and fungicide.

Conclusion

The field experiment concluded that soil microbial population and enzyme activity were affected by inorganic amendments. Less inorganic fertilizers and no fertilizers improve soil microbial activities and soil enzyme activities.

The epigenetic regulation of lncRNA TUG1 has garnered significant attention in the context of diabetes and its associated disorders. TUG1′s multifaceted roles in gene expression modulation, and cellular differentiation, and it plays a major role in the growth of diabetes and the issues that are related to it due to pathological processes. In diabetes, aberrant epigenetic modifications can lead to dysregulation of TUG1 expression, contributing to disrupted insulin signaling, impaired glucose metabolism, and beta-cell dysfunction. Moreover, it has been reported that TUG1 contributes to the development of problems linked to diabetes, such as nephropathy, retinopathy, and cardiovascular complications, through epigenetically mediated mechanisms. Understanding the epigenetic regulations of TUG1 offers novel insights into the primary molecular mechanisms of diabetes and provides a possible path for healing interventions. Targeting epigenetic modifications associated with TUG1 holds promise for restoring proper gene expression patterns, ameliorating insulin sensitivity, and mitigating the inception and development of diabetic associative diseases. This review highlights the intricate epigenetic landscape that governs TUG1 expression in diabetes, encompassing DNA methylation and alterations in histone structure, as well as microRNA interactions.

Oesophagostomum spp. (Family: Chabertiidae) is keeping a low profile in terms of severity in Bangladesh while maintaining economic loss through disguise within sheep and goats. The study was performed to identify prevalence, confirmation of species through morphology and morphometry followed by phylogeny using ITS2 and COX1 genes. In total 384 slaughterhouse-sourced small and large intestines were pooled from Mymensingh, Kishoreganj, Netrokona, Sherpur and Tangail districts of Mymensingh division. Followed by isolation, O. columbianum and O. asperum were identified following their key morphological features. Notably, O. asperum was first time detected in Bangladesh. The overall prevalence of Oesophagostomum spp. was found 60.93%. The prevalence of O. columbianum (64.95%) was almost double than that of O. asperum (35.04%). Among several characters, only the distance between anus to tail tip showed a significant morphological disparity in female. The Neighbor-joining (NJ) phylogenic trees based on ITS2 and COX1 genes confirmed the study species. The first time identified O. asperum along with morphometry and phylogeny will add value to the fact that nematodes are invisibly present with high prevalence in this country. This study will help to draw specific attention to command a practical control strategy for intervening in economic loss.

Atherosclerosis is potentially correlated with several cardiac disorders that are greatly associated with cellular oxidative stress generation, inflammation, endothelial cells dysfunction, and many cardiovascular complications. Berberine is a natural isoquinoline alkaloid compound that widely modulates pathogenesis of atherosclerosis through its different curative potentials. This in silico screening study was designed to confirm the potent restorative properties of berberine chloride as a multitarget-mediated alkaloid against the CVDs and their complications through screening, identifying, visualizing, and evaluating its binding models, affinities, and interactions toward several CVDs-related targets as direct and/or indirect-mediated signals via inhibiting cellular ER stress and apoptotic signals and activating autophagy pathway. The drug-likeness properties of berberine were predicted using the computational QSAR/ADMET and Lipinski’s RO5 analyses as well as in silico molecular docking simulations. The potent berberine-binding modes, residues-interaction patterns, and free energies of binding scores towards several CVDs-related targets were estimated using molecular docking tools. Furthermore, the pharmacokinetic properties and toxicological features of berberine were clearly determined. According to this in silico virtual screening study, berberine chloride could restore cardiac function and improve pathogenic features of atherosclerotic CVDs through alleviating ER stress and apoptotic signals, activating autophagy, improving insulin sensitivity, decreasing hyperglycemia and dyslipidemia, increasing intracellular RCT signaling, attenuating oxidative stress and vascular inflammation, and upregulating cellular antioxidant defenses in many cardiovascular tissues. In this in silico study, berberine chloride greatly modulated several potent CVDs-related targets, including SIGMAR1, GRP78, CASP3, BECN1, PIK3C3, SQSTM1/p62, LC3B, GLUT3, INSR, LDLR, LXRα, PPARγ, IL1β, IFNγ, iNOS, COX-2, MCP-1, IL10, GPx1, and SOD3.

Quercus species are one of the medicinal plants that commonly used in the treatment of different diseases. Quercus coccifera (Q. coccifera) is part of the Quercus species which grow in Jordan and used in traditional folklore medicine. The aim of this study is to confirm the ability of (Q. coccifera) leaves extracts to exert anticancer activity.

In this study, an extraction method of the dried-leaves using different polarity solvents was used. Extracts were pre-evaluated for antioxidant and anticancer activities while active extracts were used to measure half maximal effective concentration (EC₅₀) against 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and Half-maximal inhibitory concentration (IC₅₀) against cancer cells.

Methanol, boiled and microwaved water extracts had greater than 80 % antioxidant activity, and the strongest activity, of more than 99 %, was boiled water extract. Similarly, the pre-evaluation treatments of cancer cell lines indicated a strong biological activity of more than 70 % from the previously mentioned extracts, and the highest activity, of greater than 90 %, was from boiled water extracts against all cancer cell lines. The highest EC₅₀ against DPPH was obtained by using 0.009 mg/ml boiled water extracts, which was lower than positive control quercetin. In the same manner, lung, breast, and prostate cancer cell lines were highly affected by boiled water extracts with IC₅₀ of 14.1, 7.2, and 25.1 µg/ml, respectively, and a selectivity index (SI) of greater than 4.71.

Q. coccifera leaves extracts show promising ability to be a source of a new anticancer therapeutics.

Cosmetic products may be exposed to microbial contamination during storage or transport, and to avoid the risk of microbial growth, manufacturers add preservative compounds as a protection for the product from spoilage. The Microbial Challenge test is a procedure to evaluate the preservative efficacy by challenging the product with testing microorganisms to determine the quality of preservation. In this study, thirty-two cosmetics products used for body and skin care were collected from markets and pharmacies in Mecca region, these products are subjected to microbiological analysis, results show that most samples are contaminated except six samples. Non contaminated samples were subjected to European Pharmacopeia 7.0 standards. Results show that two samples, foaming gel and body and face cream are failed to demonstrate the required microbiocidal effect against the S.aureus test species, results recorded 1.21 x 10⁵ and 6.80 x 10⁵ (CFU/ml) respectively at the second day of incubation, other products: shower gel, hand wash, body lotion and shampoo demonstrate that required microbiocidal effect against the test species during day 2, 7, 14 until day 28th. The microbial count number is less than 10 during all incubation periods. To prevent contamination in cosmetics, manufacturers are required to add a good preservative system to the products and examine them before sale. Due to the high percentage of microbial contamination in cosmetics in Mecca region and for consumers safety, this study is prepared.

This study aimed to characterize chitin extracted from Indonesia mangrove crab (Scylla serrata) shells, as well as to assess its in vitro cytotoxic, antioxidant, and HMG CoA reductase inhibitory potentials. In silico molecular docking, molecular dynamic, and ADMET prediction analyses were also carried out. Chitin was extracted from mangrove crab shells using deproteination and demineralization processes, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) characterization are then performed. The MTT method was further tested in a study of cell viability, while in vitro method was used to assess HMG CoA reductase inhibitory and antioxidant activities. The extracted chitin was found to have a moderate level of cytotoxic and antioxidant activities. In vitro studies showed that it has an IC₅₀ of 36,65 ± 0,082 μg/mL as an HMG CoA reductase inhibitor, and decreased enzyme activity by 68.733 % at 100 μg/mL as a concentration. Furthermore, in the in silico study, chitin showed a strong affinity to several targets, including HMG CoA reductase, HMG synthase, LDL receptor, PPAR-alfa, and HCAR-2 with binding energies of −5.7; −5.8; −3.6; −5.6; −4.6 kcal/mol, respectively. Based on the ADMET properties, it had non-toxic molecules, which were absorbed and distributed across the blood-brain barrier. The molecular dynamics (MD) simulation also showed that it remained stable in the active sites of HMG CoA reductase receptor for 100 ns. These results indicated that chitin from Indonesian mangrove crab shells can be used to develop more potent HMG CoA reductase inhibitor with antioxidant and cytotoxic activities for effective dyslipidemia therapy.

Camels are highly suited for severe desert conditions and able to provide most of the natural products like urine, which has been used as alternative medicine to treat diverse infections and disorders. There is, however, a shortage and paucity of scientific reviews highlighting the antifungal, antibacterial and antiviral effects of camel urine. By better understanding its antimicrobial characteristics, our overarching aim is to provide an exhaustive overview of this valuable natural product by synthesizing and summarizing data on the efficacy of this biofluid and also describing the potential substances exhibiting antimicrobial properties. We searched three databases in order to point out relevant articles (Web of Science, Scopus and Google Scholar) until December 2022. Research articles of interest evaluating the antimicrobial effects of camel urine were selected. Overall, camel urine furnished promising antibacterial activities against gram-positive bacteria, namely Staphylococcus aureus (30 mm), Bacillus cereus (22 mm), Bacillus subtilis (25 mm) and Micrococcus luteus (21 mm), as well as gram-negative bacteria, especially Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter cloacae, and Salmonella spp., without forgetting its efficiency on Mycobacterium tuberculosis as well. The excretion also showed its potency against H1N1 virus, vesicular stomatitis virus and middle east respiratory syndrome coronavirus. Similarly, the camel urine featured strong antifungal activity against Candida albicans, Aspergillus niger, Aspergillus flavus and dermatophytes with a minimal inhibitory concentration of 0.625 μg/ml against Trichophyton violaceum, 2.5 μg/ml against Microsporum canis and 1.25 μg/ml against Trichophyton rubrum and Trichophyton mentagrophytes. This comprehensive review will be valuable for researchers interested in investigating the potential of camel urine in the development of novel broad-spectrum key molecules targeting a wide range of drug-resistant pathogenic microorganisms.

In terms of the economics and public health, chronic wounds exert a significant detrimental impact on the health care system. Bacterial infections, which cause the formation of highly resistant biofilms that elude standard antibiotics, are the main cause of chronic, non-healing wounds. Numerous studies have shown that phytochemicals are effective in treating a variety of diseases, and traditional medicinal plants often include important chemical groups such alkaloids, phenolics, tannins, terpenes, steroids, flavonoids, glycosides, and fatty acids. These substances are essential for scavenging free radicals which helps in reducing inflammation, fending off infections, and hastening the healing of wounds. Bacterial species can survive in chronic wound conditions because biofilms employ quorum sensing as a communication technique which regulates the expression of virulence components. Fortunately, several phytochemicals have anti-QS characteristics that efficiently block QS pathways, prevent drug-resistant strains, and reduce biofilm development in chronic wounds. This review emphasizes the potential of phytocompounds as crucial agents for alleviating bacterial infections and promoting wound healing by reducing the inflammation in chronic wounds, exhibiting potential avenues for future therapeutic approaches to mitigate the healthcare burden provided by these challenging conditions.