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A 4 km daily gridded meteorological dataset for China from 2000 to 2020. 2000 年至 2020 年中国 4 公里日网格气象数据集。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-14 DOI: 10.1038/s41597-024-04029-x
Jielin Zhang, Bo Liu, Siqing Ren, Wenqi Han, Yongxia Ding, Shouzhang Peng

Multi-variate gridded meteorological data with high spatial resolution play a key role in studies related to climate change. This study constructed a 4 km daily gridded meteorological dataset for mainland of China (China Daily Meteorological Dataset; CDMet) from 2000 to 2020. The dataset includes nine meteorological variables: 2-meter air temperature (maximum, minimum, and mean temperatures), total precipitation, skin temperature, 10-meter wind speed, relative humidity, surface pressure, and sunshine duration. CDMet was generated using an adaptive interpolation scheme, which employed thin-plate spline and random forest methods to construct the interpolation model. Six combinations of location and terrain information were designed and used as covariates in the model together with reanalysis data. Validation with independent observation stations and existing datasets showed that CDMet has acceptable accuracy, reasonable seasonal variability, and precise spatial distribution, and its accuracy is comparable to that of other datasets. Due to its comprehensive variables and high resolution, CDMet can be used as input data for hydrological, agricultural, and ecological models.

高空间分辨率的多变量网格气象数据在气候变化相关研究中发挥着关键作用。本研究构建了 2000 年至 2020 年中国大陆 4 公里日网格气象数据集(China Daily Meteorological Dataset; CDMet)。数据集包括九个气象变量:2 米气温(最高气温、最低气温和平均气温)、总降水量、皮肤温度、10 米风速、相对湿度、地面气压和日照时间。CDMet 采用自适应插值方案生成,该方案使用薄板样条和随机森林方法构建插值模型。设计了六种位置和地形信息组合,并与再分析数据一起用作模型的协变量。独立观测站和现有数据集的验证表明,CDMet 具有可接受的精度、合理的季节变化和精确的空间分布,其精度与其他数据集相当。由于 CDMet 变量全面、分辨率高,可用作水文、农业和生态模型的输入数据。
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引用次数: 0
A transcriptome data set for comparing skin, muscle and dorsal root ganglion between acute and chronic postsurgical pain rats. 用于比较急性和慢性术后疼痛大鼠皮肤、肌肉和背根神经节的转录组数据集。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-14 DOI: 10.1038/s41597-024-04078-2
Xiao-Yan Meng, Lan Bu, Ling Shen, Kun-Ming Tao

Chronic postsurgical pain (CPSP), with a high prevalence and rising epidemic of opioids crisis, is typically derived from acute postoperative pain. Our knowledge on the forming of chronic pain mostly derives from mechanistic studies of pain processing in the brain and spinal cord circuits, yet most pharmacological interventions targeting CNS came to be unhelpful in preventing CPSP. Revealing the peripheral mechanisms behind the transition from acute to chronic pain after surgery could shine a light on the novel analgesic regimens. Based on two recognized animal models in simulation of acute and chronic postsurgical pain, we provide a next-generation RNA sequencing (RNA-seq) data set to evaluate the time-course transcriptomic variation in the tissue of skin, muscle and dorsal root ganglion (DRG) in these two pain models. The aim of this study is to identify the potential origin and mechanism of the persistent postoperative pain, and further to explore effective and safer analgesic regimens for surgical patients.

慢性术后疼痛(CPSP)通常源于急性术后疼痛,其发病率高,阿片类药物危机日益严重。我们对慢性疼痛形成的认识主要来自于对大脑和脊髓回路疼痛处理的机理研究,然而大多数针对中枢神经系统的药物干预对预防 CPSP 毫无帮助。揭示手术后急性疼痛向慢性疼痛转变的外周机制,可以为新型镇痛方案提供启示。基于两种公认的模拟急性和慢性术后疼痛的动物模型,我们提供了新一代 RNA 测序(RNA-seq)数据集,以评估这两种疼痛模型中皮肤、肌肉和背根神经节(DRG)组织的时程转录组变化。这项研究的目的是确定术后持续疼痛的潜在起源和机制,并进一步探索对手术患者更有效、更安全的镇痛方案。
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引用次数: 0
Chromosome-level genome assembly of banaba (Lagerstroemia speciosa L.). 巴纳巴(Lagerstroemia speciosa L.)染色体级基因组组装。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-14 DOI: 10.1038/s41597-024-04109-y
Zhiting Wan, Tangchun Zheng, Ming Cai, Jia Wang, Huitang Pan, Tangren Cheng, Qixiang Zhang

Lagerstroemia speciosa is a famous medicinal and ornamental plant of the Lagerstroemia genus, with large and gorgeous flowers and a flowering period of 3 to 5 months. L. speciosa extracts have been used for many years in folk medicine to treat diabetes. Here, we used PacBio and Hi-C sequencing technologies to obtain a high-quality whole genome map of L. speciosa at chromosome level. The assembled genome is 306.76 Mb, with a scaffold N50 of 13.03 Mb. 98.75% of contigs were anchored to 24 pseudochromosomes, and 38.58% of the contigs (118.11 Mb) were identified as repeats. 93.54% of the 29,351 protein-coding genes were annotated. In addition, 146 miRNAs, 511 tRNAs, 2,733 rRNAs and 679 snRNAs were annotated. This high-quality genome assembly provides a valuable resource for understanding the species evolution in the Lythraceae family and promoting the study of important traits of L. speciosa.

Lagerstroemia speciosa 是一种著名的药用和观赏植物,属于 Lagerstroemia 属,花朵硕大艳丽,花期为 3 至 5 个月。多年来,L. speciosa 提取物一直被用于治疗糖尿病的民间药物中。在此,我们利用 PacBio 和 Hi-C 测序技术,在染色体水平上获得了高质量的 L. speciosa 全基因组图谱。组装的基因组为 306.76 Mb,支架 N50 为 13.03 Mb。98.75%的等位基因锚定在 24 个假染色体上,38.58%的等位基因(118.11 Mb)被鉴定为重复序列。在 29 351 个蛋白质编码基因中,有 93.54% 的基因得到了注释。此外,还注释了 146 个 miRNA、511 个 tRNA、2,733 个 rRNA 和 679 个 snRNA。这一高质量的基因组组装为了解枸杞科植物的物种进化和促进对枸杞重要性状的研究提供了宝贵的资源。
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引用次数: 0
The chromosome-level genome assembly and annotation of an invasive forest pest Obolodiplosis robiniae. 入侵森林害虫 Obolodiplosis robiniae 的染色体级基因组组装和注释。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-14 DOI: 10.1038/s41597-024-04037-x
Lan Huang, Li Wang, Hui-Quan Sun, Wen-Xia Huai, Ruo-Zhu Lin, Shu-Jun Wei, Yan-Xia Yao

Biological invasion is a major global problem, leading to the loss of biodiversity and species extinction, and causing huge economic losses to countries. Obolodiplosis robiniae is a major invasive forest pest that has caused economic losses in Asia and Europe. Here, the chromosome- level genome of O. robiniae was assembled using the PacBio platform and Hi-C technology. A contig-level genome with a length of 199.49 Mb and a contig N50 of 4.66 Mb was assembled. Approximately 98.05% of contigs were successfully anchored to four chromosomes using Hi-C assisted genome assembly. The genome integrity was assessed to be 90.3% based on BUSCOs analysis. The high-quality genome provides valuable data for the study of invasive species, and a foundation for the understanding the biology and ecology of O. robiniae.

生物入侵是一个重大的全球性问题,它导致生物多样性丧失和物种灭绝,并给各国造成巨大的经济损失。Robiniae 是一种主要的入侵性森林害虫,给亚洲和欧洲造成了经济损失。在这里,我们利用 PacBio 平台和 Hi-C 技术组装了 O. robiniae 的染色体级基因组。组装的等位基因长度为 199.49 Mb,等位基因 N50 为 4.66 Mb。利用 Hi-C 辅助基因组组装技术,约 98.05% 的等位基因成功锚定到四条染色体上。根据 BUSCOs 分析评估,基因组完整性为 90.3%。高质量的基因组为研究入侵物种提供了宝贵的数据,也为了解 O. robiniae 的生物学和生态学奠定了基础。
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引用次数: 0
Genome assembly at chromosome scale with telomere ends for Pearlspot, Etroplus suratensis. 珠斑(Etroplus suratensis)带端粒末端的染色体尺度基因组组装。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-13 DOI: 10.1038/s41597-024-04096-0
Vinaya Kumar Katneni, Karthic Krishnan, Sudheesh K Prabhudas, Roja Jayaraman, Nida Quraishi, Kumaraguru Vasagam, Ashok Kumar Jangam, Jesudhas Raymond Jani Angel, Nimisha Kaikkolante, Kumaravel Jayaraman, S Shekhar Mudagandur

The pearlspot, Etroplus suratensis is a climate resilient cichlid fish that exhibits unusual adaptation to salinity. The fish is able to complete full life cycle in diverse salinity habitats ranging from fresh water to marine environments. High-quality primary and phased genome assemblies were generated for pearlspot fish using PacBio HiFi and Arima HiC sequencing technologies, for the first time. The primary assembly is highly contiguous with contig N50 length of 36 Mb. The final assembly is of 1.247 Gb with N50 length of 51.57 Mb and 98% of the genome length anchored to 24 chromosomes. The genome was assessed to be 99.9% complete based on BUSCO evaluation and was predicted to contain 52.96% repeat elements. We have predicted 27,192 protein encoding genes, of which 21,580 were functionally annotated. The genome offers an invaluable resource to understand adaptation of pearlspot fish to diverse salinity habitats.

珍珠斑(Etroplus suratensis)是一种气候适应能力很强的慈鲷鱼,对盐度有着不同寻常的适应能力。这种鱼能够在从淡水到海洋环境的不同盐度生境中完成整个生命周期。利用 PacBio HiFi 和 Arima HiC 测序技术,首次为珍珠斑鱼生成了高质量的初级和阶段性基因组组装。初选序列高度连续,序列 N50 长度为 36 Mb。最终组装结果为 1.247 Gb,N50 长度为 51.57 Mb,基因组长度的 98% 固定在 24 条染色体上。根据 BUSCO 评估,该基因组的完整度为 99.9%,预计含有 52.96% 的重复元件。我们预测了 27,192 个蛋白质编码基因,其中 21,580 个已进行了功能注释。该基因组为了解珍珠斑鱼对不同盐度生境的适应性提供了宝贵的资源。
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引用次数: 0
Chromosome-level genome assembly of the cave leech Sinospelaeobdella cavatuses (Hirudinea: Haemadipsidae). 洞穴水蛭(Sinospelaeobdella cavatuses)(蛭科:Haemadipsidae)染色体组水平的基因组组装。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-13 DOI: 10.1038/s41597-024-04007-3
Binbin Yao, Delong Guan, Lulu Yang, Tao Jiang, Lu Zhao, Muhammad Salabat Khan, Sheng-Quan Xu

Leeches are famous for their high medical value and wide environmental adaptability. Among them, cave leeches are a very fascinating and rare group, which is an important component in the study of adaptive evolution of leeches. However, no study has yet reported a reference genome for this group. In this study, we assembled a high-quality chromosome-level genome of the cave terrestrial leech Sinospelaeobdella cavatuses, through Illumina and PacBio sequencing, alongside chromosome conformation capture techniques. The resulting genome spans 153.67 Mb across 9 pseudochromosomes(range: 11.33 to 23.53 Mb), with a mounting rate of up to 95.37% and features an N50 length of 17.15 Mb. This genome is composed of 35.16% repetitive elements and contains 21180 predicted protein-coding genes. Decoding the S. cavatuses genome not only promotes future studies on study of its phylogeny, evolution, and behavior, but also provides valuable resources for in-depth investigation on adaptive evolution of leech.

水蛭以其极高的医疗价值和广泛的环境适应性而闻名于世。其中,穴居水蛭是一个非常迷人和罕见的群体,是研究水蛭适应性进化的重要组成部分。然而,目前还没有关于该类水蛭参考基因组的研究报告。在这项研究中,我们通过Illumina和PacBio测序技术以及染色体构象捕获技术,组装了洞穴陆生水蛭Sinospelaeobdella cavatuses的高质量染色体组基因组。测序结果显示,9个假染色体的基因组跨度为153.67 Mb(范围:11.33至23.53 Mb),安装率高达95.37%,N50长度为17.15 Mb。该基因组由 35.16% 的重复元件组成,包含 21180 个预测的蛋白质编码基因。水蛭基因组的解码不仅有助于今后对其系统发育、进化和行为的研究,也为深入研究水蛭的适应性进化提供了宝贵的资源。
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引用次数: 0
Comprehensive experimental datasets of quasicrystals and their approximants. 准晶体及其近似值的综合实验数据集。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-13 DOI: 10.1038/s41597-024-04043-z
Erina Fujita, Chang Liu, Asuka Ishikawa, Tomoya Mato, Koichi Kitahara, Ryuji Tamura, Kaoru Kimura, Ryo Yoshida, Yukari Katsura

Quasicrystals are solid-state materials that typically exhibit unique symmetries, such as icosahedral or decagonal diffraction symmetry. They were first discovered in 1984. Over the past four decades of quasicrystal research, around 100 stable quasicrystals have been discovered. In recent years, machine learning has been employed to explore quasicrystals with unique properties inherent to quasiperiodic systems. However, the lack of open data on quasicrystal composition, structure, and physical properties has hindered the widespread use of machine learning in quasicrystal research. This study involves a comprehensive literature review and manual data extraction to develop open datasets consisting of composition, structure types, phase diagrams, and sample fabrication processes for a wide range of stable and metastable quasicrystals and approximant crystals, as well as the temperature-dependent thermal, electrical, and magnetic properties.

准晶体是一种固态材料,通常表现出独特的对称性,例如二十面体或十边形衍射对称性。它们于 1984 年首次被发现。在过去 40 年的准晶体研究中,已发现约 100 种稳定的准晶体。近年来,人们利用机器学习来探索具有准周期系统固有的独特性质的准晶体。然而,由于缺乏有关准晶体组成、结构和物理性质的公开数据,阻碍了机器学习在准晶体研究中的广泛应用。本研究通过全面的文献综述和手动数据提取,开发了开放数据集,其中包括各种稳定和瞬变准晶体和近似晶体的组成、结构类型、相图和样品制造工艺,以及随温度变化的热、电和磁特性。
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引用次数: 0
Whole genome sequencing of CRISPR/Cas9-engineered NF-κB reporter mice for validation and variant discovery. 对 CRISPR/Cas9 改造的 NF-κB 报告小鼠进行全基因组测序,以进行验证和变异发现。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-13 DOI: 10.1038/s41597-024-04064-8
Guruswamy Mahesh, Erik W Martin, Mohammad Aqdas, Kyu-Seon Oh, Myong-Hee Sung

Targeted knockout, mutations, or knock-in of genomic DNA fragments in model organisms have been used widely for functional and cell-tracking studies. The desired genetic perturbation is often accomplished by recombination-based or CRISPR/Cas9-based genome engineering. For validating the intended genetic modification, a local region surrounding the targeted locus is typically examined based on enzymatic cleavage and consequent length patterns, e.g. in a Southern analysis. Despite its wide use, this approach is open to incomplete and ambiguous readouts. With decreasing costs of high-throughput sequencing, it is becoming feasible to consider a large-scale validation of a new strain after a targeted genetic perturbation. Here we describe a dataset of whole-genome sequences and the variant analysis results from four novel reporter mouse strains. This served to validate the strains and identified all the off-target effects on the genome, thereby increasing the genetic diversity of genomic sequences over those represented in the public databases for inbred mice.

模式生物基因组 DNA 片段的靶向基因敲除、突变或基因敲入已被广泛用于功能和细胞追踪研究。所需的基因扰动通常通过基于重组或 CRISPR/Cas9 的基因组工程来实现。为了验证预期的基因修饰,通常会根据酶裂解和随之而来的长度模式(如在 Southern 分析中)对目标基因座周围的局部区域进行检查。尽管这种方法被广泛使用,但其读数不完整、不明确。随着高通量测序成本的降低,考虑对定向遗传扰动后的新菌株进行大规模验证正变得可行。在这里,我们描述了四个新型报告小鼠品系的全基因组序列数据集和变异分析结果。这有助于验证这些品系,并确定基因组的所有非目标效应,从而增加基因组序列的遗传多样性,超过近交系小鼠公共数据库中的基因组序列。
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引用次数: 0
Chromosome-level genome assembly of Indo-Pacific king mackerel (Scomberomorus guttatus). 印度-太平洋马鲛鱼(Scomberomorus guttatus)染色体级基因组组装。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-13 DOI: 10.1038/s41597-024-04110-5
Yan Gao, Sailan Liu, Qilin Gutang, Chengzhang Li, Xiaoqi Lin, Bo Liang, Ping Li, Jianqing Lin, Wenhua Liu

The Indo-Pacific king mackerel, scientifically known as Scomberomorus guttatus, is a valued marine species that holds significant commercial importance in the Indo-Pacific region. However, the lack of genomic resources has hindered a comprehensive understanding of this species. In this study, we constructed a genome of Indo-Pacific king mackerel at the chromosome level using a combination of PacBio HiFi reads and a chromosome contact map (Hi-C). The resulting genome had high contig and scaffold N50 values of 8.84 Mb and 32.9 Mb, respectively. In the genome assembly, which is 797.66 Mb in size and consists of 24 chromosomes, we also identified 35.89% repetitive elements and predicted 25,886 protein-coding genes. Our study not only benefits to reveal the possible mechanism of adaptive evolution in this fish, but also offers insight for the future sustainable management of these valuable biological resources.

印度-太平洋鲭王,学名为Scomberomorus guttatus,是一种珍贵的海洋物种,在印度-太平洋地区具有重要的商业价值。然而,基因组资源的缺乏阻碍了对该物种的全面了解。在这项研究中,我们结合 PacBio HiFi 读数和染色体接触图谱(Hi-C),在染色体水平上构建了印度洋-太平洋鲭王的基因组。所得到的基因组具有较高的等位基因和支架 N50 值,分别为 8.84 Mb 和 32.9 Mb。在由 24 条染色体组成、大小为 797.66 Mb 的基因组组装中,我们还发现了 35.89% 的重复元件,并预测了 25,886 个蛋白编码基因。我们的研究不仅有助于揭示这种鱼类适应性进化的可能机制,还为这些宝贵生物资源未来的可持续管理提供了启示。
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引用次数: 0
High-resolution topography reveals morphological changes of Stromboli volcano following the July 2024 eruption. 高分辨率地形图显示了 2024 年 7 月爆发后斯特龙博利火山的形态变化。
IF 5.8 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-11-12 DOI: 10.1038/s41597-024-04098-y
Riccardo Civico, Tullio Ricci, Alessandro Cecili, Piergiorgio Scarlato

The July 2024 eruption of Stromboli volcano has been characterised by the manifestation, at variable intensity, of the entire repertoire of volcanic events that Stromboli volcano is capable of, and is by far the one that has most changed the morphology of the crater terrace and of the Sciara del Fuoco slope in the last decades. We present the results of an Unoccupied Aircraft System (UAS) survey performed on 14 July 2024 and consisting of 4,988 visible and thermal photographs coupled with Structure-from-Motion photogrammetry that allowed us to produce a high-resolution (0.2 m/pixel) Digital Surface Model (DSM). We documented the profound morphological changes of the Stromboli volcano resulting from the 4-11 July 2024 eruption and obtained elevation and volume change estimates by differencing our survey and a UAS-derived pre-event surface (24 May 2024).

2024 年 7 月斯特龙博利火山喷发的特点是以不同的强度表现出斯特龙博利火山所能发生的全部火山活动,而且是迄今为止在过去几十年中对火山口平台和 Sciara del Fuoco 斜坡形态改变最大的一次。我们介绍了 2024 年 7 月 14 日进行的无人驾驶航空器系统(UAS)勘测的结果,包括 4,988 张可见光和热照片,以及结构移动摄影测量,使我们能够制作出高分辨率(0.2 米/像素)的数字地表模型(DSM)。我们记录了 2024 年 7 月 4 日至 11 日喷发造成的斯特龙博利火山的深刻形态变化,并通过差分我们的勘测结果和无人机系统得出的喷发前地表(2024 年 5 月 24 日),获得了海拔高度和体积变化的估计值。
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引用次数: 0
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