Pub Date : 2024-09-16DOI: 10.1038/s41597-024-03831-x
Matthias Luh, Thomas Blank
Battery degradation is critical to the cost-effectiveness and usability of battery-powered products. Aging studies help to better understand and model degradation and to optimize the operating strategy. Nevertheless, there are only a few comprehensive and freely available aging datasets for these applications. To our knowledge, the dataset1 presented in the following is one of the largest published to date. It contains over 3 billion data points from 228 commercial NMC/C+SiO lithium-ion cells aged for more than a year under a wide range of operating conditions. We investigate calendar and cyclic aging and also apply different driving cycles to cells. The dataset1 includes result data (such as the remaining usable capacity or impedance measured in check-ups) and raw data (i.e., measurement logs with two-second resolution). The data can be used in a wide range of applications, for example, to model battery degradation, gain insight into lithium plating, optimize operating strategies, or test battery impedance or state estimation algorithms using machine learning or Kalman filtering.
{"title":"Comprehensive battery aging dataset: capacity and impedance fade measurements of a lithium-ion NMC/C-SiO cell.","authors":"Matthias Luh, Thomas Blank","doi":"10.1038/s41597-024-03831-x","DOIUrl":"10.1038/s41597-024-03831-x","url":null,"abstract":"<p><p>Battery degradation is critical to the cost-effectiveness and usability of battery-powered products. Aging studies help to better understand and model degradation and to optimize the operating strategy. Nevertheless, there are only a few comprehensive and freely available aging datasets for these applications. To our knowledge, the dataset<sup>1</sup> presented in the following is one of the largest published to date. It contains over 3 billion data points from 228 commercial NMC/C+SiO lithium-ion cells aged for more than a year under a wide range of operating conditions. We investigate calendar and cyclic aging and also apply different driving cycles to cells. The dataset<sup>1</sup> includes result data (such as the remaining usable capacity or impedance measured in check-ups) and raw data (i.e., measurement logs with two-second resolution). The data can be used in a wide range of applications, for example, to model battery degradation, gain insight into lithium plating, optimize operating strategies, or test battery impedance or state estimation algorithms using machine learning or Kalman filtering.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11405776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-14DOI: 10.1038/s41597-024-03846-4
Zaiyuan Li, Guangchang Ma, Chao Tang, Haibo Wen, Conghui Liu, Bo Liu, Xi Qiao, Tao Jin, Wanqiang Qian, Fanghao Wan, Zhengqiang Peng, Zhi Gong
Brontispa longissima is a highly destructive pest that affects coconut and ornamental palm plants. It is widely distributed across Southeast and East Asia and the Pacific region, causing production losses of up to 50-70%. While control methods and ecological phenomena have been the primary focus of research, there is a significant lack of studies on the molecular mechanisms underlying these ecological phenomena. The absence of a reference genome has also hindered the development of new molecular-targeted control technologies. In this study, we conducted a karyotype analysis of B. longissima and assembled the first high-quality chromosome-level genome. The assembled genome is 582.24 Mb in size, with a scaffold N50 size of 63.81 Mb, consisting of 10 chromosomes and a GC content of 33.71%. The BUSCO assessment indicated a completeness estimate of 98.1%. A total of 23,051 protein-coding genes were predicted. Our study provides a valuable genomic resource for understanding the mechanisms of adaptive evolution and facilitates the development of new molecular-targeted control methods for B. longissima.
{"title":"A chromosome-level genome assembly of the Brontispa longissima.","authors":"Zaiyuan Li, Guangchang Ma, Chao Tang, Haibo Wen, Conghui Liu, Bo Liu, Xi Qiao, Tao Jin, Wanqiang Qian, Fanghao Wan, Zhengqiang Peng, Zhi Gong","doi":"10.1038/s41597-024-03846-4","DOIUrl":"10.1038/s41597-024-03846-4","url":null,"abstract":"<p><p>Brontispa longissima is a highly destructive pest that affects coconut and ornamental palm plants. It is widely distributed across Southeast and East Asia and the Pacific region, causing production losses of up to 50-70%. While control methods and ecological phenomena have been the primary focus of research, there is a significant lack of studies on the molecular mechanisms underlying these ecological phenomena. The absence of a reference genome has also hindered the development of new molecular-targeted control technologies. In this study, we conducted a karyotype analysis of B. longissima and assembled the first high-quality chromosome-level genome. The assembled genome is 582.24 Mb in size, with a scaffold N50 size of 63.81 Mb, consisting of 10 chromosomes and a GC content of 33.71%. The BUSCO assessment indicated a completeness estimate of 98.1%. A total of 23,051 protein-coding genes were predicted. Our study provides a valuable genomic resource for understanding the mechanisms of adaptive evolution and facilitates the development of new molecular-targeted control methods for B. longissima.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1038/s41597-024-03738-7
Christoph Anders, Sidratul Moontaha, Samik Real, Bert Arnrich
While individuals fail to assess their mental health subjectively in their day-to-day activities, the recent development of consumer-grade wearable devices has enormous potential to monitor daily workload objectively by acquiring physiological signals. Therefore, this work collected consumer-grade physiological signals from twenty-four participants, following a four-hour cognitive load elicitation paradigm with self-chosen tasks in uncontrolled environments and a four-hour mental workload elicitation paradigm in a controlled environment. The recorded dataset of approximately 315 hours consists of electroencephalography, acceleration, electrodermal activity, and photoplethysmogram data balanced across low and high load levels. Participants performed office-like tasks in the controlled environment (mental arithmetic, Stroop, N-Back, and Sudoku) with two defined difficulty levels and in the uncontrolled environments (mainly researching, programming, and writing emails). Each task label was provided by participants using two 5-point Likert scales of mental workload and stress and the pairwise NASA-TLX questionnaire. This data is suitable for developing real-time mental health assessment methods, conducting research on signal processing techniques for challenging environments, and developing personal cognitive load assistants.
{"title":"Unobtrusive measurement of cognitive load and physiological signals in uncontrolled environments.","authors":"Christoph Anders, Sidratul Moontaha, Samik Real, Bert Arnrich","doi":"10.1038/s41597-024-03738-7","DOIUrl":"10.1038/s41597-024-03738-7","url":null,"abstract":"<p><p>While individuals fail to assess their mental health subjectively in their day-to-day activities, the recent development of consumer-grade wearable devices has enormous potential to monitor daily workload objectively by acquiring physiological signals. Therefore, this work collected consumer-grade physiological signals from twenty-four participants, following a four-hour cognitive load elicitation paradigm with self-chosen tasks in uncontrolled environments and a four-hour mental workload elicitation paradigm in a controlled environment. The recorded dataset of approximately 315 hours consists of electroencephalography, acceleration, electrodermal activity, and photoplethysmogram data balanced across low and high load levels. Participants performed office-like tasks in the controlled environment (mental arithmetic, Stroop, N-Back, and Sudoku) with two defined difficulty levels and in the uncontrolled environments (mainly researching, programming, and writing emails). Each task label was provided by participants using two 5-point Likert scales of mental workload and stress and the pairwise NASA-TLX questionnaire. This data is suitable for developing real-time mental health assessment methods, conducting research on signal processing techniques for challenging environments, and developing personal cognitive load assistants.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1038/s41597-024-03858-0
Xuan Li, Suvratha Jayaprasad, Elisabet Einarsdottir, Steven J B Cooper, Alexander Suh, Takeshi Kawakami, Octavio Manuel Palacios-Gimenez
Morabine grasshoppers in the Vandiemenella viatica species group, which show karyotype diversity, have been studied for their ecological distribution and speciation in relation to their genetic and chromosomal diversity. They are good models for studying sex chromosome evolution as "old" and newly emerged sex chromosomes co-exist within the group. Here we present a reference genome for the viatica19 chromosomal race, that possesses the ancestral karyotype within the group. Using PacBio HiFi and Hi-C sequencing, we generated a chromosome-level assembly of 4.09 Gb in span, scaffold N50 of 429 Mb, and complete BUSCO score of 98.1%, containing 10 pseudo-chromosomes. We provide Illumina datasets of males and females, used to identify the X chromosome. The assembly contains 19,034 predicted protein-coding genes, and a total of 75.21% of repetitive DNA sequences. By leveraging HiFi reads, we mapped the genome-wide distribution of methylated bases (5mC and 6 mA). This comprehensive assembly offers a robust reference for morabine grasshoppers and supports further research into speciation and sex chromosome diversification within the group and its related species.
{"title":"Chromosome-level genome assembly of the morabine grasshopper Vandiemenella viatica19.","authors":"Xuan Li, Suvratha Jayaprasad, Elisabet Einarsdottir, Steven J B Cooper, Alexander Suh, Takeshi Kawakami, Octavio Manuel Palacios-Gimenez","doi":"10.1038/s41597-024-03858-0","DOIUrl":"https://doi.org/10.1038/s41597-024-03858-0","url":null,"abstract":"<p><p>Morabine grasshoppers in the Vandiemenella viatica species group, which show karyotype diversity, have been studied for their ecological distribution and speciation in relation to their genetic and chromosomal diversity. They are good models for studying sex chromosome evolution as \"old\" and newly emerged sex chromosomes co-exist within the group. Here we present a reference genome for the viatica19 chromosomal race, that possesses the ancestral karyotype within the group. Using PacBio HiFi and Hi-C sequencing, we generated a chromosome-level assembly of 4.09 Gb in span, scaffold N50 of 429 Mb, and complete BUSCO score of 98.1%, containing 10 pseudo-chromosomes. We provide Illumina datasets of males and females, used to identify the X chromosome. The assembly contains 19,034 predicted protein-coding genes, and a total of 75.21% of repetitive DNA sequences. By leveraging HiFi reads, we mapped the genome-wide distribution of methylated bases (5mC and 6 mA). This comprehensive assembly offers a robust reference for morabine grasshoppers and supports further research into speciation and sex chromosome diversification within the group and its related species.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Changjiang Estuary and adjacent East China Sea are well-known hypoxic aquatic environments. Eutrophication-driven hypoxia frequently occurs in coastal areas, posing a major threat to the ecological environment, including altering community structure and metabolic processes of marine organisms, and enhancing diversion of energy shunt into microbial communities. However, the responses of microbial communities and their metabolic pathways to coastal hypoxia remain poorly understood. Here, we studied the microbial communities collected from spatiotemporal samplings using metagenomic sequencing in the Changjiang Estuary and adjacent East China Sea. This generated 1.31 Tbp of metagenomics data, distributed across 103 samples corresponding to 8 vertical profiles. We further reported 1,559 metagenome-assembled genomes (MAGs), of which 508 were high-quality MAGs (Completeness > 90% and Contamination < 10%). Phylogenomic analysis classified them into 181 archaeal and 1,378 bacterial MAGs. These results provided a valuable metagenomic dataset available for further investigation of the effects of hypoxia on marine microorganisms.
{"title":"Recovery of 1559 metagenome-assembled genomes from the East China Sea's low-oxygen region.","authors":"Shujing Liu, Quanrui Chen, Congcong Hou, Changjie Dong, Xuanyun Qiu, Kai Tang","doi":"10.1038/s41597-024-03850-8","DOIUrl":"10.1038/s41597-024-03850-8","url":null,"abstract":"<p><p>The Changjiang Estuary and adjacent East China Sea are well-known hypoxic aquatic environments. Eutrophication-driven hypoxia frequently occurs in coastal areas, posing a major threat to the ecological environment, including altering community structure and metabolic processes of marine organisms, and enhancing diversion of energy shunt into microbial communities. However, the responses of microbial communities and their metabolic pathways to coastal hypoxia remain poorly understood. Here, we studied the microbial communities collected from spatiotemporal samplings using metagenomic sequencing in the Changjiang Estuary and adjacent East China Sea. This generated 1.31 Tbp of metagenomics data, distributed across 103 samples corresponding to 8 vertical profiles. We further reported 1,559 metagenome-assembled genomes (MAGs), of which 508 were high-quality MAGs (Completeness > 90% and Contamination < 10%). Phylogenomic analysis classified them into 181 archaeal and 1,378 bacterial MAGs. These results provided a valuable metagenomic dataset available for further investigation of the effects of hypoxia on marine microorganisms.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A significant hurdle in untargeted lipid/metabolomics research lies in the absence of reliable, cross-validated spectral libraries, leading to a considerable portion of LC-MS features being labeled as unknowns. Despite continuous advancement in annotation tools and libraries, it is important to safeguard, publish and share acquired data through public repositories. Embracing this trend of data sharing not only promotes efficient resource utilization but also paves the way for future repurposing and in-depth analysis; ultimately advancing our comprehension of Covid-19 and other diseases. In this work, we generated an extensive MS-dataset of 39 Covid-19 infected patients versus age- and gender-matched 39 healthy controls. We implemented state of the art acquisition techniques including IDA and SWATH-DIA to ensure a thorough insight in the lipidome and metabolome, ensuring a repurposable dataset.
非靶向脂质/代谢组学研究的一大障碍在于缺乏可靠的交叉验证光谱库,导致相当一部分 LC-MS 特征被标记为未知。尽管注释工具和谱库在不断进步,但通过公共资料库保护、发布和共享所获得的数据仍然非常重要。拥抱数据共享这一趋势不仅能促进资源的有效利用,还能为未来的再利用和深入分析铺平道路,最终促进我们对 Covid-19 和其他疾病的理解。在这项工作中,我们生成了 39 名 Covid-19 感染者与 39 名年龄和性别匹配的健康对照者的大量 MS 数据集。我们采用了最先进的采集技术,包括 IDA 和 SWATH-DIA,以确保深入了解脂质组和代谢组,从而确保数据集的可再利用性。
{"title":"A comprehensive IDA and SWATH-DIA Lipidomics and Metabolomics dataset: SARS-CoV-2 case control study.","authors":"Ammar Tahir, Agnes Draxler, Tamara Stelzer, Amelie Blaschke, Brenda Laky, Marton Széll, Jessica Binar, Viktoria Bartak, Laura Bragagna, Lina Maqboul, Theresa Herzog, Rainer Thell, Karl-Heinz Wagner","doi":"10.1038/s41597-024-03822-y","DOIUrl":"https://doi.org/10.1038/s41597-024-03822-y","url":null,"abstract":"<p><p>A significant hurdle in untargeted lipid/metabolomics research lies in the absence of reliable, cross-validated spectral libraries, leading to a considerable portion of LC-MS features being labeled as unknowns. Despite continuous advancement in annotation tools and libraries, it is important to safeguard, publish and share acquired data through public repositories. Embracing this trend of data sharing not only promotes efficient resource utilization but also paves the way for future repurposing and in-depth analysis; ultimately advancing our comprehension of Covid-19 and other diseases. In this work, we generated an extensive MS-dataset of 39 Covid-19 infected patients versus age- and gender-matched 39 healthy controls. We implemented state of the art acquisition techniques including IDA and SWATH-DIA to ensure a thorough insight in the lipidome and metabolome, ensuring a repurposable dataset.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1038/s41597-024-03849-1
RunZhao Fang, Xiao Tian, Dan Liang, Peng Zhang
Achelura yunnanensis is a destructive pest of forests, causing substantial damage on tree growth and severe economic losses. Additionally, as a daytime-active moth, this species also holds important scientific value for investigating the genetic mechanisms governing day-night activity patterns of Lepidoptera. To facilitate effective pest control and deepen our understanding of the diurnal behavior's genetic basis of moths, genomic data for this species are crucial. In this study, we present a chromosome-level reference genome of A. yunnanensis (368.15 Mb in 32 chromosomes; scaffold N50 = 12.61 Mb; BUSCO completeness = 98.0%). Genome annotation shows that the new assembly comprises 37.10% (136.55 Mb) repetitive elements, 1,828 non-coding RNAs, and 15,523 protein-coding genes. Genes involved in lipid metabolism and xenobiotics biodegradation and metabolism, such as cytochrome P450 families, experienced significant expansion in the A. yunnanensis genome. The chromosome-level genome of A. yunnanensis provides a valuable genomic resource for devising novel pest control strategies, and will also help to study the genetic mechanism of the shift of diurnal behavior in Lepidoptera.
{"title":"Chromosome-level genome assembly of the forest pest Achelura yunnanensis (Lepidoptera: Zygaenidae).","authors":"RunZhao Fang, Xiao Tian, Dan Liang, Peng Zhang","doi":"10.1038/s41597-024-03849-1","DOIUrl":"https://doi.org/10.1038/s41597-024-03849-1","url":null,"abstract":"<p><p>Achelura yunnanensis is a destructive pest of forests, causing substantial damage on tree growth and severe economic losses. Additionally, as a daytime-active moth, this species also holds important scientific value for investigating the genetic mechanisms governing day-night activity patterns of Lepidoptera. To facilitate effective pest control and deepen our understanding of the diurnal behavior's genetic basis of moths, genomic data for this species are crucial. In this study, we present a chromosome-level reference genome of A. yunnanensis (368.15 Mb in 32 chromosomes; scaffold N50 = 12.61 Mb; BUSCO completeness = 98.0%). Genome annotation shows that the new assembly comprises 37.10% (136.55 Mb) repetitive elements, 1,828 non-coding RNAs, and 15,523 protein-coding genes. Genes involved in lipid metabolism and xenobiotics biodegradation and metabolism, such as cytochrome P450 families, experienced significant expansion in the A. yunnanensis genome. The chromosome-level genome of A. yunnanensis provides a valuable genomic resource for devising novel pest control strategies, and will also help to study the genetic mechanism of the shift of diurnal behavior in Lepidoptera.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1038/s41597-024-03845-5
Ethan A Brown, Ronald A Hellenthal, Michael B Mahon, Samantha L Rumschlag, Jason R Rohr
Range maps are used to estimate the geographic extent of taxa, providing valuable information for biodiversity and conservation research and management. Freshwater macroinvertebrates are not well-represented in the range map literature relative to freshwater vertebrates. To address this knowledge gap, we provide range maps for 1158 freshwater macroinvertebrate genera based on two decades of publicly available occurrence data from the USEPA National Aquatic Resource Surveys, which included 11,628 sites and 6,906,990 organisms across the contiguous USA. Maps were created by applying unweighted and weighted pair group method with arithmetic mean clustering and single-linkage clustering algorithms to the occurrence data and creating three layers of polygons from the minimum convex hulls of clusters. A total of 25 freshwater macroinvertebrate classes are represented in the range map dataset. Most mapped genera were insects (394/1158), followed by malacostracans (242/1158), polychaetes (182/1158), and bivalves (121/1158). Additionally, we provide waterbody type percent occupancy data for all genera, detailing how genera are partitioned between boatable streams, wadeable streams, inland lakes, Laurentian Great Lakes, and coastal estuaries.
{"title":"Range maps and waterbody occupancy data for 1158 freshwater macroinvertebrate genera in the contiguous USA.","authors":"Ethan A Brown, Ronald A Hellenthal, Michael B Mahon, Samantha L Rumschlag, Jason R Rohr","doi":"10.1038/s41597-024-03845-5","DOIUrl":"10.1038/s41597-024-03845-5","url":null,"abstract":"<p><p>Range maps are used to estimate the geographic extent of taxa, providing valuable information for biodiversity and conservation research and management. Freshwater macroinvertebrates are not well-represented in the range map literature relative to freshwater vertebrates. To address this knowledge gap, we provide range maps for 1158 freshwater macroinvertebrate genera based on two decades of publicly available occurrence data from the USEPA National Aquatic Resource Surveys, which included 11,628 sites and 6,906,990 organisms across the contiguous USA. Maps were created by applying unweighted and weighted pair group method with arithmetic mean clustering and single-linkage clustering algorithms to the occurrence data and creating three layers of polygons from the minimum convex hulls of clusters. A total of 25 freshwater macroinvertebrate classes are represented in the range map dataset. Most mapped genera were insects (394/1158), followed by malacostracans (242/1158), polychaetes (182/1158), and bivalves (121/1158). Additionally, we provide waterbody type percent occupancy data for all genera, detailing how genera are partitioned between boatable streams, wadeable streams, inland lakes, Laurentian Great Lakes, and coastal estuaries.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1038/s41597-024-03839-3
Logan S Schwartz, Ruth L Saxl, Tim Stearns, Maria Telpoukhovskaia, Jennifer J Trowbridge
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family of cytokines and has been found to have anti-inflammatory and pro-inflammatory properties in various cellular and disease contexts. OSM signals through two receptor complexes, one of which includes OSMRβ. Here, we investigated OSM-OSMRβ signaling in adult mouse hematopoietic stem cells (HSCs) using the conditional Osmrfl/fl mouse model B6;129-Osmrtm1.1Nat/J. We crossed Osmrfl/fl mice to interferon-inducible Mx1-Cre, which is robustly induced in adult HSCs. From these mice, we isolated HSCs by flow cytometry, stimulated with recombinant OSM or vehicle for 1 hour, and assessed gene expression changes in control versus Osmr knockout HSCs by RNA-seq. This data may be utilized to investigate OSMRβ -dependent and -independent OSM signaling as well as the transcriptional effects of an IL-6 family cytokine on mouse HSCs to further define its anti-inflammatory versus pro-inflammatory properties.
{"title":"Oncostatin M receptor-dependent signaling assessed by RNA sequencing in mouse hematopoietic stem cells.","authors":"Logan S Schwartz, Ruth L Saxl, Tim Stearns, Maria Telpoukhovskaia, Jennifer J Trowbridge","doi":"10.1038/s41597-024-03839-3","DOIUrl":"10.1038/s41597-024-03839-3","url":null,"abstract":"<p><p>Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family of cytokines and has been found to have anti-inflammatory and pro-inflammatory properties in various cellular and disease contexts. OSM signals through two receptor complexes, one of which includes OSMRβ. Here, we investigated OSM-OSMRβ signaling in adult mouse hematopoietic stem cells (HSCs) using the conditional Osmr<sup>fl/fl</sup> mouse model B6;129-Osmr<sup>tm1.1Nat</sup>/J. We crossed Osmr<sup>fl/fl</sup> mice to interferon-inducible Mx1-Cre, which is robustly induced in adult HSCs. From these mice, we isolated HSCs by flow cytometry, stimulated with recombinant OSM or vehicle for 1 hour, and assessed gene expression changes in control versus Osmr knockout HSCs by RNA-seq. This data may be utilized to investigate OSMRβ -dependent and -independent OSM signaling as well as the transcriptional effects of an IL-6 family cytokine on mouse HSCs to further define its anti-inflammatory versus pro-inflammatory properties.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":null,"pages":null},"PeriodicalIF":5.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11392925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142294489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}