Pub Date : 2018-08-31DOI: 10.15587/2519-8025.2018.141406
R. Suleimanova, D. Melnichuk, L. Kalachniuk
{"title":"Liver fatty acids composition in the sterlet of different ages","authors":"R. Suleimanova, D. Melnichuk, L. Kalachniuk","doi":"10.15587/2519-8025.2018.141406","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.141406","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86887273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-28DOI: 10.15587/2519-8025.2018.141154
A. Dorofeeva
The aim of the study was to reveal the peculiarities of the intestinal microflora in patients with early and late debut of ulcerative colitis. Methods of research: theoretical – analysis of scientific-methodical and special literature; experimental methods – collection of samples and DNA isolation, determination of oligonucleotide primers, polymerase chain reaction (PCR), determination of microbiom composition; mathematical - the method of average values. Results : In the course of the study, it was determined that the number of Bacteroidetes in patients with late onset of UC development decreased in the Ukrainian population, and the level of Actinobacteria increased. Changes in the microbiota in patients with UC with different localization of the inflammatory process were analyzed. It is established that the composition of microbial types significantly differs not only depending on the age of onset of the disease, but also on the localization of the inflammatory process in the intestine. As the prevalence of UC increased, the level of Actinobacteria was highest in patients with left-sided bowel disease and late onset of UC. Whereas in patients with total intestinal lesion, both early and late development of the UC revealed a maximum decrease in Faecalibacterium prausnitzii. Conclusion : UC patients experience intestinal dysbiosis. The level of Actinobacteria is increased in patients with late onset of UC development, and the number of Bacteroidetes decreases. In patients with early and late onset of the disease, multidirectional changes in the intestinal microbiome were detected, the number of Akkermansia muciniphila increased and Faecalibacterium prausnitzii decreased, which could lead to the development and progression of the disease. The ratio of Firmicutes / Bacteroidetes may be an additional marker for assessing the severity of intestinal dysbiosis in UC patients. At the same time, the prevalence of the pathological process in the intestine largely determines the therapeutic tactics and prognosis of patients with UC
{"title":"Assessment of changes in the intestinal microbiome in the late debut of ulcerative colitis","authors":"A. Dorofeeva","doi":"10.15587/2519-8025.2018.141154","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.141154","url":null,"abstract":"The aim of the study was to reveal the peculiarities of the intestinal microflora in patients with early and late debut of ulcerative colitis. Methods of research: theoretical – analysis of scientific-methodical and special literature; experimental methods – collection of samples and DNA isolation, determination of oligonucleotide primers, polymerase chain reaction (PCR), determination of microbiom composition; mathematical - the method of average values. Results : In the course of the study, it was determined that the number of Bacteroidetes in patients with late onset of UC development decreased in the Ukrainian population, and the level of Actinobacteria increased. Changes in the microbiota in patients with UC with different localization of the inflammatory process were analyzed. It is established that the composition of microbial types significantly differs not only depending on the age of onset of the disease, but also on the localization of the inflammatory process in the intestine. As the prevalence of UC increased, the level of Actinobacteria was highest in patients with left-sided bowel disease and late onset of UC. Whereas in patients with total intestinal lesion, both early and late development of the UC revealed a maximum decrease in Faecalibacterium prausnitzii. Conclusion : UC patients experience intestinal dysbiosis. The level of Actinobacteria is increased in patients with late onset of UC development, and the number of Bacteroidetes decreases. In patients with early and late onset of the disease, multidirectional changes in the intestinal microbiome were detected, the number of Akkermansia muciniphila increased and Faecalibacterium prausnitzii decreased, which could lead to the development and progression of the disease. The ratio of Firmicutes / Bacteroidetes may be an additional marker for assessing the severity of intestinal dysbiosis in UC patients. At the same time, the prevalence of the pathological process in the intestine largely determines the therapeutic tactics and prognosis of patients with UC","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84643560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-20DOI: 10.15587/2519-8025.2018.140857
L. Yukhymenko, M. Makarchuk
The aim of the research is to develop differential scales for assessing the functioning of the brain-heart system of student youth, which are based on individual neurodynamic and neurovegetative characteristics of information processing.Methods A comprehensive research was performed on the EEG activity of the brain, the spectral characteristics of the regulation of cardiac rhythm (CR), electrical resistance of the skin (ERS), the assessment of the success of the studying (SS) and the level of anxiety in students with different individual neurodynamic properties of higher nervous activity (HNA) in a resting state and during the processing of information on a computer.Results of the research In the resting state the interdependencies between the studied indicators were not established. During the task of processing and differentiating of information, a connection was established between the individual neurodynamic properties of HNA, the EEG activity of the brain, the power of the spectrum of autonomic regulation of HR, ERS, SS and reactive anxiety (RA).Conclusions On the basis of the application of scaling technology to the quantitative and qualitative characteristics of neurodynamic and neurovegetative properties of psychophysiological functions, five levels of functioning of the “brain-heart” system have been identified and recommendations have been developed for the corresponding studying regimes. We believe that the use of differential scales opens up new possibilities of individualization of education, typology of the evaluation of the functioning of the “brain-heart” system during mental activity, and also broadens the prognostic criteria for its resistance to stress-induced studying factors in students
{"title":"The evaluation of the functional system of \"brain-heart\" during the processing of information in students by the method of differential scales","authors":"L. Yukhymenko, M. Makarchuk","doi":"10.15587/2519-8025.2018.140857","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.140857","url":null,"abstract":"The aim of the research is to develop differential scales for assessing the functioning of the brain-heart system of student youth, which are based on individual neurodynamic and neurovegetative characteristics of information processing.Methods A comprehensive research was performed on the EEG activity of the brain, the spectral characteristics of the regulation of cardiac rhythm (CR), electrical resistance of the skin (ERS), the assessment of the success of the studying (SS) and the level of anxiety in students with different individual neurodynamic properties of higher nervous activity (HNA) in a resting state and during the processing of information on a computer.Results of the research In the resting state the interdependencies between the studied indicators were not established. During the task of processing and differentiating of information, a connection was established between the individual neurodynamic properties of HNA, the EEG activity of the brain, the power of the spectrum of autonomic regulation of HR, ERS, SS and reactive anxiety (RA).Conclusions On the basis of the application of scaling technology to the quantitative and qualitative characteristics of neurodynamic and neurovegetative properties of psychophysiological functions, five levels of functioning of the “brain-heart” system have been identified and recommendations have been developed for the corresponding studying regimes. We believe that the use of differential scales opens up new possibilities of individualization of education, typology of the evaluation of the functioning of the “brain-heart” system during mental activity, and also broadens the prognostic criteria for its resistance to stress-induced studying factors in students","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"497 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80023534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-20DOI: 10.15587/2519-8025.2018.140861
O. Zorina, S. Protas
{"title":"Hygienic assessment of tap water quality by sanitary-chemical parameters and improvement of the scientific methodological approaches of their assessment in accordance with the requirements of the european legislation","authors":"O. Zorina, S. Protas","doi":"10.15587/2519-8025.2018.140861","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.140861","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81613339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-30DOI: 10.15587/2519-8025.2018.135852
D. Rakhmetov, O. Bondarchuk, O. Vergun, Valentyna Fishchenko
{"title":"Biochemical characteristics of aboveground phytomass of plant of the genus Astragalus L. in the right-bank of forest-steppe of Ukraine","authors":"D. Rakhmetov, O. Bondarchuk, O. Vergun, Valentyna Fishchenko","doi":"10.15587/2519-8025.2018.135852","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.135852","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73180565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-27DOI: 10.15587/2519-8025.2018.135426
O. Rakovets, I. Kuznetsov, M. Osyp, I. Kotsan
{"title":"Electric activity of the human brain in individuals with different egoism-altruism level","authors":"O. Rakovets, I. Kuznetsov, M. Osyp, I. Kotsan","doi":"10.15587/2519-8025.2018.135426","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.135426","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"7 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72574654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-27DOI: 10.15587/2519-8025.2018.135657
Е. Аrystarkhova, L. Romantschuk, Y. Dankevych, O. Zhytova
Aim . Revelation of the influence of ammonia from surface water supply sources on formation of their toxicity, determined by indices of motor activity of young lacustrine frogs (Rana ridibunda Pallas, 1771). Methods . Biotesting was conducted by the method of «time sampling» with preliminary exposure of individuals for 12 hours (n=30) in test samples of water, taken from the Denishevsky water storage basin and Otsechne water intake of the river Teterev and also in samples of settled water from water pipe as a control. The indices of water toxicity were calculated by reactions of typical and non-typical activity of individuals. The ammonia content was determined by the photometric method with Nessler reagent. The correlation and regression analysis was realized by the standard computer program MO Excel 2003. Results . There are established the effects of the ammonia influence on the toxicity of test waters that is proved by the values of determination coefficients, received for 3-year period (R 2 at level 0,3893 for the water storage basin and 0,2814 for water intake) and correlation (r of middle degree 0,6240 and 0,5305 respectively). There are constructed the graphs, expressed by the equations of rectilinear regression (y=52,535x+16,207 and y=50,917x+21,067), that gives a possibility to prognosticate toxicity levels of waters as to the ammonia content for the water storage basin and water intake. Conclusions . There were revealed the correlations of the indices of toxicity of the testing waters with the ammonia content that in 2014 (0,6939 – in the Denishevsky water storage basin and 0,6803 – in the Otsechne water intake) reached more degree than in 2012 (0,6413 and 0,4281) and 2013 (0,6556 and 0,5083) years, respectively that is conditioned by increasing ammonification processes. There was fixed a tendency of increasing the force of the ammonia influence (from 41,12 to 48,15 % in waters of the water storage basin and from 18,33 to 46,28 % – water intake) on the toxicity of these waters. In the 3-years period of the studies, there was testified the presence of the reliable ammonia influence of the toxicity of waters in the water storage basin at level 38,93 % (F=3,0811; p ≤0,05, ), and also its absence in the water intake 28,14 % (F=1,5663). There were given the equations of rectilinear regression and graphic images of the dependencies that allow to calculate the ammonia content (0,58 mg/dm 3 and 0,64) at reaching 50 % level of water toxicity of lacustrine frogs
{"title":"Influence of ammonia of waters from the surface sources of water supply on their toxicity for Rana Ridibunda (Pallas, 1771)","authors":"Е. Аrystarkhova, L. Romantschuk, Y. Dankevych, O. Zhytova","doi":"10.15587/2519-8025.2018.135657","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.135657","url":null,"abstract":"Aim . Revelation of the influence of ammonia from surface water supply sources on formation of their toxicity, determined by indices of motor activity of young lacustrine frogs (Rana ridibunda Pallas, 1771). Methods . Biotesting was conducted by the method of «time sampling» with preliminary exposure of individuals for 12 hours (n=30) in test samples of water, taken from the Denishevsky water storage basin and Otsechne water intake of the river Teterev and also in samples of settled water from water pipe as a control. The indices of water toxicity were calculated by reactions of typical and non-typical activity of individuals. The ammonia content was determined by the photometric method with Nessler reagent. The correlation and regression analysis was realized by the standard computer program MO Excel 2003. Results . There are established the effects of the ammonia influence on the toxicity of test waters that is proved by the values of determination coefficients, received for 3-year period (R 2 at level 0,3893 for the water storage basin and 0,2814 for water intake) and correlation (r of middle degree 0,6240 and 0,5305 respectively). There are constructed the graphs, expressed by the equations of rectilinear regression (y=52,535x+16,207 and y=50,917x+21,067), that gives a possibility to prognosticate toxicity levels of waters as to the ammonia content for the water storage basin and water intake. Conclusions . There were revealed the correlations of the indices of toxicity of the testing waters with the ammonia content that in 2014 (0,6939 – in the Denishevsky water storage basin and 0,6803 – in the Otsechne water intake) reached more degree than in 2012 (0,6413 and 0,4281) and 2013 (0,6556 and 0,5083) years, respectively that is conditioned by increasing ammonification processes. There was fixed a tendency of increasing the force of the ammonia influence (from 41,12 to 48,15 % in waters of the water storage basin and from 18,33 to 46,28 % – water intake) on the toxicity of these waters. In the 3-years period of the studies, there was testified the presence of the reliable ammonia influence of the toxicity of waters in the water storage basin at level 38,93 % (F=3,0811; p ≤0,05, ), and also its absence in the water intake 28,14 % (F=1,5663). There were given the equations of rectilinear regression and graphic images of the dependencies that allow to calculate the ammonia content (0,58 mg/dm 3 and 0,64) at reaching 50 % level of water toxicity of lacustrine frogs","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76064003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-27DOI: 10.15587/2519-8025.2018.135625
O. Zorina
The aim of current research was to make the hygienic assessment of sanitary and chemical indicators of potable tap water quality in Ukraine in regions with the shortage of potable water, where drinking water is used from underground low-quality sources or the big water pipelines. While conducting the research, the following methods were used: sanitary-chemical, hygienic monitoring, and mathematical statistics. It was determined, that the groundwater and hybrid water supply are used for provision of shallow water regions with potable tap water. In some regions of Ukraine, the water of centralized supply of drinking water from underground sources is of inadequate quality, which is due to the following indicators: nitrates, fluorine, dry residue, total hardness, chlorides, sulfates, iron, and manganese. Quantity of indicators for groundwater, which levels doesn’t meet the hygienic requirements, can vary from 1-2 to 6-8, in average - 6 indicators. Detected exceedances of maximum permitted hygienic requirements reach up to 2-4,6 times. With the use of combined water supply in the drinking water of consumers of the Communal Enterprise "Berdyanskvodokanal" maximum content was detected: nickel -10MAC, selen-5MAC, permanganate demand — 1,2MAC (before transporting by the big water pipeline, dry residue — 3,5MAC, total hardness—3,9 MAC, sulfates — 7,8MAC, colour — 2MAC, permanganate demand — 1,4 MAC (because of the water pollution in local surface water source). Reasonability of membrane technologies implementing for potable tap water purification has been proved. For the purpose of solving the crisis in the sphere of potable water supply considering the European legislation, the following actions need to be taken: to improve the national water legislation on water bodies protection from pollution and on assessment of potable water quality, as well as to implement the efficient modern technologies of water treatment and equip the measuring laboratories
{"title":"Hygienic assessment of sanitary and chemical indicators of potable tap water quality in shallow water regions of Ukraine","authors":"O. Zorina","doi":"10.15587/2519-8025.2018.135625","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.135625","url":null,"abstract":"The aim of current research was to make the hygienic assessment of sanitary and chemical indicators of potable tap water quality in Ukraine in regions with the shortage of potable water, where drinking water is used from underground low-quality sources or the big water pipelines. While conducting the research, the following methods were used: sanitary-chemical, hygienic monitoring, and mathematical statistics. It was determined, that the groundwater and hybrid water supply are used for provision of shallow water regions with potable tap water. In some regions of Ukraine, the water of centralized supply of drinking water from underground sources is of inadequate quality, which is due to the following indicators: nitrates, fluorine, dry residue, total hardness, chlorides, sulfates, iron, and manganese. Quantity of indicators for groundwater, which levels doesn’t meet the hygienic requirements, can vary from 1-2 to 6-8, in average - 6 indicators. Detected exceedances of maximum permitted hygienic requirements reach up to 2-4,6 times. With the use of combined water supply in the drinking water of consumers of the Communal Enterprise \"Berdyanskvodokanal\" maximum content was detected: nickel -10MAC, selen-5MAC, permanganate demand — 1,2MAC (before transporting by the big water pipeline, dry residue — 3,5MAC, total hardness—3,9 MAC, sulfates — 7,8MAC, colour — 2MAC, permanganate demand — 1,4 MAC (because of the water pollution in local surface water source). Reasonability of membrane technologies implementing for potable tap water purification has been proved. For the purpose of solving the crisis in the sphere of potable water supply considering the European legislation, the following actions need to be taken: to improve the national water legislation on water bodies protection from pollution and on assessment of potable water quality, as well as to implement the efficient modern technologies of water treatment and equip the measuring laboratories","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72555258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-12DOI: 10.15587/2519-8025.2018.133392
N. Maznyk, T. Sypko, Nataliya Pshenichna, V. Starenkiy, Iryna Krugova
{"title":"Radiation-induced chromosome aberrations in oncogynecological patients and patients with head and neck cancer undergoing radiotherapy on a linear accelerator","authors":"N. Maznyk, T. Sypko, Nataliya Pshenichna, V. Starenkiy, Iryna Krugova","doi":"10.15587/2519-8025.2018.133392","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.133392","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78252274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-08DOI: 10.15587/2519-8025.2018.133186
I. Korshikov, L. Boyko, Oleg Krasnoshtan, O. Suslova, Antonina Mazur
{"title":"Diversity and vitality of tree species in the street plantations of Kryvyi Rih city","authors":"I. Korshikov, L. Boyko, Oleg Krasnoshtan, O. Suslova, Antonina Mazur","doi":"10.15587/2519-8025.2018.133186","DOIUrl":"https://doi.org/10.15587/2519-8025.2018.133186","url":null,"abstract":"","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75177264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：