Pub Date : 2021-09-30DOI: 10.15587/2519-8025.2021.241865
Nigar Hidayet Pashayeva, T. Suleymanov, Y. Kerimov, E. Gasimov, F. Rzayev
The aim of this work was to study of diagnostic signs of the morphological and anatomical structure of Veronica crista-galli Steven. from the flora of Azerbaijan. Materials and methods. The samples for research were collected during their flowering time in June 2018, in the Ismailli region of the Republic of Azerbaijan. Plant samples were fixed in a solution made in 0.1 M phosphate buffer (pH=7.4), containing 2.5 % glutar-aldehyde, 2.5 % paraformal-aldehyde and 0.1 % picric acid. In the next stage was the preparation of block and their filling in Araldite – Epon according to the TEM method. Results. The leaf is simple, lower part is short-petiolate and upper is sessile. The surface, on both sides of the leaf, is reliefly, and 7–8 conductive veins are clearly visible. The lower and upper sides of the leaf, and also margin, are strewn with multicellular hairs. The calyx of the flower consists of two sepals which grown together at the base, covered with simple multicellular hairs. The stalk in is a long filiform. The corolla of flower consists of 4 petals which grown together at the base and 2 stamens attached to the tube of the corolla. On the epidermis, cells with sinuous and bead-like walls, numerous stomata of the stavrocytic type, capitate hairs are visible. From the cross section of the leaf, it is visible that palisade tissue at the upper and sponge tissue at the bottom. Conclusions. As a result of morphological and anatomical studies, it was revealed that diagnostic signs of plant raw material can be: Present of multicellular hairs on the leaf blade; The location of the capsule between the sepals; Stavrocytic type of the stoma structure; The bead-like walls of the epidermis; Capitate hairs on the epidermis; Sepals covered by hairs. The established anatomical diagnostic features can be used for the drafting of the normative document on the plant raw materials and for identification of plant raw material of Veronica crista-galli
{"title":"Morphological and anatomical properties of Veronica crista-galli Steven","authors":"Nigar Hidayet Pashayeva, T. Suleymanov, Y. Kerimov, E. Gasimov, F. Rzayev","doi":"10.15587/2519-8025.2021.241865","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.241865","url":null,"abstract":"The aim of this work was to study of diagnostic signs of the morphological and anatomical structure of Veronica crista-galli Steven. from the flora of Azerbaijan. \u0000Materials and methods. The samples for research were collected during their flowering time in June 2018, in the Ismailli region of the Republic of Azerbaijan. Plant samples were fixed in a solution made in 0.1 M phosphate buffer (pH=7.4), containing 2.5 % glutar-aldehyde, 2.5 % paraformal-aldehyde and 0.1 % picric acid. In the next stage was the preparation of block and their filling in Araldite – Epon according to the TEM method. \u0000Results. The leaf is simple, lower part is short-petiolate and upper is sessile. The surface, on both sides of the leaf, is reliefly, and 7–8 conductive veins are clearly visible. The lower and upper sides of the leaf, and also margin, are strewn with multicellular hairs. The calyx of the flower consists of two sepals which grown together at the base, covered with simple multicellular hairs. The stalk in is a long filiform. The corolla of flower consists of 4 petals which grown together at the base and 2 stamens attached to the tube of the corolla. On the epidermis, cells with sinuous and bead-like walls, numerous stomata of the stavrocytic type, capitate hairs are visible. From the cross section of the leaf, it is visible that palisade tissue at the upper and sponge tissue at the bottom. \u0000Conclusions. As a result of morphological and anatomical studies, it was revealed that diagnostic signs of plant raw material can be: Present of multicellular hairs on the leaf blade; The location of the capsule between the sepals; Stavrocytic type of the stoma structure; The bead-like walls of the epidermis; Capitate hairs on the epidermis; Sepals covered by hairs. \u0000The established anatomical diagnostic features can be used for the drafting of the normative document on the plant raw materials and for identification of plant raw material of Veronica crista-galli","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75939104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.15587/2519-8025.2021.239920
I. Herasymets, Liudmila Fira, I. Medvid
The aim. To study the effect of a dry extract from reishi mushrooms on the activity of lipoperoxidation and oxidative modification of proteins under the conditions of a simulated paracetamol hepatitis in rats. Materials and methods. The study was performed on white male rats. The animals were divided into 10 groups, each included 6 animals. Acute hepatitis was simulated by the intragastric administration of paracetamol in the dose of 1250 mg/kg once per day (for 2 days). Correction of the pathology induced was performed with a dry extract of reishi mushrooms in the dose of 100 mg/kg of the body weight. The reference drug “Silybor” was administered in the dose of 20 mg/kg of the animal body weight. On Day 3, 7 and 10 from the beginning of the lesion, rats were euthanized using sodium barbamyl. The liver homogenate and blood serum were used for the studies. The activity of free radical oxidation processes under the conditions of acute toxic hepatitis and after the introduction of corrective factors was assessed by superoxide dismutase, catalase activity, the content of TBA-AP and OMP products. Results and discussion. The development of acute paracetamol hepatitis in rats and damage of hepatocyte membranes are indicated by an increase in the content of TBA-active products, products of oxidative modification of neutral and basic proteins in the serum and liver of animals. Simultaneously, a decrease in the activity of catalase and superoxide dismutase was observed. After correction of the pathology induced with a dry extract of reishi mushrooms a significant increase in the activity of antioxidant enzymes, a decrease in the content of lipid peroxidation products and oxidative modification of proteins in the serum and liver of the affected animals were observed. Conclusions. It has been experimentally proven that the use of dry extract of Reishi mushrooms in paracetamol hepatitis in rats caused a significant decrease in catalase and superoxide dismutase activity, a decrease in TBA-AP, neutral and basic 2,4-DNPH in the serum and liver of animals. The results of the research indicate an effective impact of reishi mushrooms dry extract on the normalization of lipoperoxidation, oxidative modification of proteins and antioxidant protection
{"title":"The study of oxidative stress indicators in rats with a simulated acute hepatitis and correction with a thick extract from reishi mushrooms","authors":"I. Herasymets, Liudmila Fira, I. Medvid","doi":"10.15587/2519-8025.2021.239920","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.239920","url":null,"abstract":"The aim. To study the effect of a dry extract from reishi mushrooms on the activity of lipoperoxidation and oxidative modification of proteins under the conditions of a simulated paracetamol hepatitis in rats. \u0000Materials and methods. The study was performed on white male rats. The animals were divided into 10 groups, each included 6 animals. Acute hepatitis was simulated by the intragastric administration of paracetamol in the dose of 1250 mg/kg once per day (for 2 days). Correction of the pathology induced was performed with a dry extract of reishi mushrooms in the dose of 100 mg/kg of the body weight. The reference drug “Silybor” was administered in the dose of 20 mg/kg of the animal body weight. On Day 3, 7 and 10 from the beginning of the lesion, rats were euthanized using sodium barbamyl. The liver homogenate and blood serum were used for the studies. The activity of free radical oxidation processes under the conditions of acute toxic hepatitis and after the introduction of corrective factors was assessed by superoxide dismutase, catalase activity, the content of TBA-AP and OMP products. \u0000Results and discussion. The development of acute paracetamol hepatitis in rats and damage of hepatocyte membranes are indicated by an increase in the content of TBA-active products, products of oxidative modification of neutral and basic proteins in the serum and liver of animals. Simultaneously, a decrease in the activity of catalase and superoxide dismutase was observed. After correction of the pathology induced with a dry extract of reishi mushrooms a significant increase in the activity of antioxidant enzymes, a decrease in the content of lipid peroxidation products and oxidative modification of proteins in the serum and liver of the affected animals were observed. \u0000Conclusions. It has been experimentally proven that the use of dry extract of Reishi mushrooms in paracetamol hepatitis in rats caused a significant decrease in catalase and superoxide dismutase activity, a decrease in TBA-AP, neutral and basic 2,4-DNPH in the serum and liver of animals. The results of the research indicate an effective impact of reishi mushrooms dry extract on the normalization of lipoperoxidation, oxidative modification of proteins and antioxidant protection","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84339181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.15587/2519-8025.2021.241245
R. Dotsenko, M. Romanko, Yevheniia Vashchyk, A. Zakhariev, A. Zemlianskyi, E. Dotsenko, N. Seliukova
The aim: to determine the effect of a preparative form of an antiemeric agent based on maduramycin and nicarbazine on the body of white rats and broiler chickens under the conditions of a subacute experiment. Materials and methods. The formulation based on maduramycin and nicarbazine was administered to rats and broiler chickens with feed for 28 days. One control and three experimental groups were formed for the experiment: Group I – animals received a complete diet without admixture of the formulation (control group), Group II – animals that were administered the formulation (by the amount of active substances) at a dose of 5.0 mg / kg, III – 25.0 mg / kg and IV – 50.0 mg / kg of feed, respectively. In order to establish the toxic effect of the formulation on the body of experimental birds on 7, 14, 28 days of the experiment and 7 days after discontinuation of the formulation, 5 heads from each group were killed under light ether anesthesia, blood samples were taken for hematological and biochemical studies. Results. During the study of the general clinical condition of rats and broiler chickens of the experimental groups, no significant changes in behavior and appearance were detected, compared with the control. Hematological parameters of broiler chickens it was found that the receipt of the formulation with feed at doses of 5, 25 and 50 mg/kg body weight, causes a decrease in hemoglobin and an increase in hematocrit in broiler chickens on day 7 of the experiment. It was found that the changes are reversible, on the 28th day of the experiment the value of these indicators was within the physiological norm. In the study of hematological and biochemical parameters of rats, it was found that the receipt of the formulation with food in doses of 25.0 and 50.0 mg/kg for 28 days, causes a decrease in hemoglobin and increase hematocrit, total protein, albumin, creatinine and urea in male rats on the 7th day of the experiment. Conclusions. Under conditions of repeated oral administration of the preparation mixture to rats and poultry at doses of 5, 25 and 50 mg/kg body weight in the absence of clinical signs of poisoning, insignificant fluctuations in hematological and biochemical parameters were identified
{"title":"The effect of a formulation based on maduramycin and nicarbazine on rats and broiler chickens in a subacute experiment","authors":"R. Dotsenko, M. Romanko, Yevheniia Vashchyk, A. Zakhariev, A. Zemlianskyi, E. Dotsenko, N. Seliukova","doi":"10.15587/2519-8025.2021.241245","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.241245","url":null,"abstract":"The aim: to determine the effect of a preparative form of an antiemeric agent based on maduramycin and nicarbazine on the body of white rats and broiler chickens under the conditions of a subacute experiment. \u0000Materials and methods. The formulation based on maduramycin and nicarbazine was administered to rats and broiler chickens with feed for 28 days. One control and three experimental groups were formed for the experiment: Group I – animals received a complete diet without admixture of the formulation (control group), Group II – animals that were administered the formulation (by the amount of active substances) at a dose of 5.0 mg / kg, III – 25.0 mg / kg and IV – 50.0 mg / kg of feed, respectively. In order to establish the toxic effect of the formulation on the body of experimental birds on 7, 14, 28 days of the experiment and 7 days after discontinuation of the formulation, 5 heads from each group were killed under light ether anesthesia, blood samples were taken for hematological and biochemical studies. \u0000Results. During the study of the general clinical condition of rats and broiler chickens of the experimental groups, no significant changes in behavior and appearance were detected, compared with the control. \u0000Hematological parameters of broiler chickens it was found that the receipt of the formulation with feed at doses of 5, 25 and 50 mg/kg body weight, causes a decrease in hemoglobin and an increase in hematocrit in broiler chickens on day 7 of the experiment. It was found that the changes are reversible, on the 28th day of the experiment the value of these indicators was within the physiological norm. \u0000In the study of hematological and biochemical parameters of rats, it was found that the receipt of the formulation with food in doses of 25.0 and 50.0 mg/kg for 28 days, causes a decrease in hemoglobin and increase hematocrit, total protein, albumin, creatinine and urea in male rats on the 7th day of the experiment. \u0000Conclusions. Under conditions of repeated oral administration of the preparation mixture to rats and poultry at doses of 5, 25 and 50 mg/kg body weight in the absence of clinical signs of poisoning, insignificant fluctuations in hematological and biochemical parameters were identified","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77306959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.15587/2519-8025.2021.235525
А. Popyk, V. Kyslychenko, V. Velma
An important place in human nutrition is occupied by omega-3, omega-6 and omega-9 fatty acids (FA). They have different effects on the human body and play a significant role in the appearance and the course of some diseases (for example, cardiovascular system, gastrointestinal tract, cancer, obesity, etc.). The aim. To study the qualitative composition and the quantitative content of fatty acids in Common Lilac (Syringa vulgaris) flowers of “Madame Lemoine” variety. Materials and methods. The fatty acid composition was studied by gas chromatography based on the formation of methyl esters of fatty acids and their subsequent determination. Results and discussion. The presence and the quantitative content of 15 fatty acids have been determined. Among them, 13 FA have been identified, namely 6 saturated fatty acids and 7 unsaturated fatty acids. The total amount of saturated fatty acids (54.65 %) significantly predominates over unsaturated fatty acids (34.81 %). Palmitic acid dominates among saturated FA (39.83 %). Linoleic acid has the highest percent among unsaturated FA (13.75 %). Conclusions. For the first time the composition of fatty acids in Common Lilac flowers of “Madame Lemoine” variety has been studied. The raw material accumulates saturated fatty acids in a significant number. Palmitic acid (C 16 : 0) – 39.83 % and linoleic acid (C 18 : 2) – 13.75 % are the dominant acids
{"title":"The study of the fatty acid composition of common lilac flowers of “Madame Lemoine” variety","authors":"А. Popyk, V. Kyslychenko, V. Velma","doi":"10.15587/2519-8025.2021.235525","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.235525","url":null,"abstract":"An important place in human nutrition is occupied by omega-3, omega-6 and omega-9 fatty acids (FA). They have different effects on the human body and play a significant role in the appearance and the course of some diseases (for example, cardiovascular system, gastrointestinal tract, cancer, obesity, etc.). \u0000The aim. To study the qualitative composition and the quantitative content of fatty acids in Common Lilac (Syringa vulgaris) flowers of “Madame Lemoine” variety. \u0000Materials and methods. The fatty acid composition was studied by gas chromatography based on the formation of methyl esters of fatty acids and their subsequent determination. \u0000Results and discussion. The presence and the quantitative content of 15 fatty acids have been determined. Among them, 13 FA have been identified, namely 6 saturated fatty acids and 7 unsaturated fatty acids. The total amount of saturated fatty acids (54.65 %) significantly predominates over unsaturated fatty acids (34.81 %). Palmitic acid dominates among saturated FA (39.83 %). Linoleic acid has the highest percent among unsaturated FA (13.75 %). \u0000Conclusions. For the first time the composition of fatty acids in Common Lilac flowers of “Madame Lemoine” variety has been studied. The raw material accumulates saturated fatty acids in a significant number. Palmitic acid (C 16 : 0) – 39.83 % and linoleic acid (C 18 : 2) – 13.75 % are the dominant acids","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83941649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.15587/2519-8025.2021.235057
Y. Kosenko, S. Bilous, N. Ostapiv, L. Zaruma
The use of antimicrobial medicines in human and veterinary medicine has led to the problem of the development of acquired antimicrobial resistance, which causes a global threat. Were described principles of tetracyclines and sulfonamides use, which are the most common among antimicrobial substances in veterinary medicinal products for the treatment of infectious diseases of food-producing and domestic animals. The aim. To substantiate the clinical relevance of antimicrobial veterinary medicinal products containing tetracyclines and sulfonamides+trimethoprim in veterinary medicine. Materials and methods. Research materials: sales reports of antimicrobial veterinary medicinal products in Ukraine for 2015–2019, EU countries, and the USA. Methods used: written and electronic survey; bibliosemantic, analytical and generalization. Results and discussion. As a result of the annual monitoring for 2015–2019 sales volumes in Ukraine, it was determined that tetracyclines (29.5–37.91 %) and sulfonamides + trimethoprim (12.1–18.7 %) were most often used in the composition of veterinary medicines. The same trend regarding the use of these classes of antimicrobials exists in many countries around the world. Factors determining the clinical relevance of these groups of substances are based on the criteria for their selection. The principle proposed by the EMA for the choice of antimicrobial veterinary medicinal products is based on the following criteria: categories of target animal species; treatment indications; the route of administration; the type of pharmaceutical formulation; the choice of a dosage regimen. Following this principle was substantiated the feasibility of tetracyclines and sulfonamides+trimethoprim use in veterinary medicine. By pharmacokinetic and pharmacodynamic parameters evaluated the rationality of the choice of antimicrobial veterinary medicines. Conclusions. The study revealed clinical efficacy and safety of tetracyclines and sulfonamides+trimethoprim as Veterinary Critically Important Antimicrobial Agents of Category D “Prudence”
{"title":"Use of tetracyclines and sulfonamides for the treatment of infectious diseases in animals","authors":"Y. Kosenko, S. Bilous, N. Ostapiv, L. Zaruma","doi":"10.15587/2519-8025.2021.235057","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.235057","url":null,"abstract":"The use of antimicrobial medicines in human and veterinary medicine has led to the problem of the development of acquired antimicrobial resistance, which causes a global threat. Were described principles of tetracyclines and sulfonamides use, which are the most common among antimicrobial substances in veterinary medicinal products for the treatment of infectious diseases of food-producing and domestic animals. \u0000The aim. To substantiate the clinical relevance of antimicrobial veterinary medicinal products containing tetracyclines and sulfonamides+trimethoprim in veterinary medicine. \u0000Materials and methods. Research materials: sales reports of antimicrobial veterinary medicinal products in Ukraine for 2015–2019, EU countries, and the USA. Methods used: written and electronic survey; bibliosemantic, analytical and generalization. \u0000Results and discussion. As a result of the annual monitoring for 2015–2019 sales volumes in Ukraine, it was determined that tetracyclines (29.5–37.91 %) and sulfonamides + trimethoprim (12.1–18.7 %) were most often used in the composition of veterinary medicines. The same trend regarding the use of these classes of antimicrobials exists in many countries around the world. Factors determining the clinical relevance of these groups of substances are based on the criteria for their selection. \u0000The principle proposed by the EMA for the choice of antimicrobial veterinary medicinal products is based on the following criteria: categories of target animal species; treatment indications; the route of administration; the type of pharmaceutical formulation; the choice of a dosage regimen. Following this principle was substantiated the feasibility of tetracyclines and sulfonamides+trimethoprim use in veterinary medicine. \u0000By pharmacokinetic and pharmacodynamic parameters evaluated the rationality of the choice of antimicrobial veterinary medicines. \u0000Conclusions. The study revealed clinical efficacy and safety of tetracyclines and sulfonamides+trimethoprim as Veterinary Critically Important Antimicrobial Agents of Category D “Prudence”","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87958954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.15587/2519-8025.2021.235936
L. Fedonyuk, Y. Stravskyy, V. Khavtur, R. Sachuk
Aim. To establish the effect of acidum succinicum on the activity of enzymes in the blood of cows after calving and the course of uterine involution. Materials and Methods. The study of enzyme activity was carried out in the Central Research Laboratory of I. Horbachevsky Ternopil National Medical University (Certificate of technical competence № 001/18, issued on September 26, 2018, valid until September 25, 2023). Results of the research. After the use of acidum succinicum in the body of cows of the experimental group, the activity of alanine aminotransferase increased by 12,0 % (p≤0,05), and the activity of aspartate aminotransferase, on the contrary, decreased by 30,0 % (p≤0,001), which indicates a positive effect on hepatocytes. Confirmation of this is the Ritis factor, which increased 1,4 times (p≤0,05). All animal experiments were conducted according to with the recommendations of ARRIVE and the British Animal Act 1986 and Directive 2010/63 / EU on the protection of animals for scientific investigation. In the body of cows of the control group, the activity of alanine and aspartateaminotransferases increased, respectively, by 14,0 % (p≤0,05) and 47,0 % (p≤0,001) against a background of a 1,2-fold decrease (p≤0,05) Ritis factor. A decrease in bilirubin content of 56,0 % (p≤0,001) in the body of cows after the use of acidum succinicum and an increase in its content by 2,2 times (p≤0,001) in the body of control cows indicates liver disease, which is accompanied by affection of its parenchyma, which may be a consequence of intoxication of their body in the last trimester of pregnancy. Conclusion. Acidum succinicum, applied to cows after calving, prevents the development of inflammation of the reproductive system, helps to restore reproductive function, reduces the duration of the service period to 80,0±2,0 days, and the insemination index to 1,3
{"title":"Prevention of subinvolution of the uterus of cows using acidum succinicum","authors":"L. Fedonyuk, Y. Stravskyy, V. Khavtur, R. Sachuk","doi":"10.15587/2519-8025.2021.235936","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.235936","url":null,"abstract":"Aim. To establish the effect of acidum succinicum on the activity of enzymes in the blood of cows after calving and the course of uterine involution. \u0000Materials and Methods. The study of enzyme activity was carried out in the Central Research Laboratory of I. Horbachevsky Ternopil National Medical University (Certificate of technical competence № 001/18, issued on September 26, 2018, valid until September 25, 2023). \u0000Results of the research. After the use of acidum succinicum in the body of cows of the experimental group, the activity of alanine aminotransferase increased by 12,0 % (p≤0,05), and the activity of aspartate aminotransferase, on the contrary, decreased by 30,0 % (p≤0,001), which indicates a positive effect on hepatocytes. Confirmation of this is the Ritis factor, which increased 1,4 times (p≤0,05). \u0000All animal experiments were conducted according to with the recommendations of ARRIVE and the British Animal Act 1986 and Directive 2010/63 / EU on the protection of animals for scientific investigation. \u0000In the body of cows of the control group, the activity of alanine and aspartateaminotransferases increased, respectively, by 14,0 % (p≤0,05) and 47,0 % (p≤0,001) against a background of a 1,2-fold decrease (p≤0,05) Ritis factor. \u0000A decrease in bilirubin content of 56,0 % (p≤0,001) in the body of cows after the use of acidum succinicum and an increase in its content by 2,2 times (p≤0,001) in the body of control cows indicates liver disease, which is accompanied by affection of its parenchyma, which may be a consequence of intoxication of their body in the last trimester of pregnancy. \u0000Conclusion. Acidum succinicum, applied to cows after calving, prevents the development of inflammation of the reproductive system, helps to restore reproductive function, reduces the duration of the service period to 80,0±2,0 days, and the insemination index to 1,3","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87057293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15587/2519-8025.2021.241538
L. Galuzinska, V. Kravchenko, G. Kravchenko, T. Briukhanova, V.Р. Fylymonenko
Medicinal plants have been widely used in the treatment of many diseases, especially in the gastroenterology, given that the use of synthetic antiulcer drugs in clinical practice often leads to the development of various kinds of side effects and, accordingly, have a large list of contraindications. The aim of this experimental work was the study of the antiulcer activity of the food concentrate of phenolic compounds of apples on the models of experimental ulcers in rats. Materials and methods. The studies included two stages, which were carried out on models: stage I – a model of alcohol–prednisolone stomach ulcer in rats, stage II – a model of indomethacin stomach ulcer in rats in accordance with the methodological recommendations of the SEC of the Ministry of Health of Ukraine. The studied concentrate was administered in a dose based on the total content of polyphenols – 9 mg per 100 g of weight. Quercetin at a dose of 5 mg / kg and ranitidine at a dose of 20 mg / kg were used as reference drugs. After replicating the models, a macroscopic examination of the gastric mucosa was carried out and the content of TBA-reactants and reduced glutathione in the stomach homogenate was determined. Results. Under pathological conditions, the therapeutic effect of the food concentrate of phenolic compounds of apples and reference drugs was comparable in terms of the degree of ulcer defect. It was proved that food concentrate of phenolic compounds of apples and reference drugs inhibit the course of lipid peroxidation reactions and support endogenous antioxidant defense systems. Conclusions. The obtained results make it possible to recommend a further study of the food concentrate of phenolic compounds of apples for the creation of a pharmaceutical preparation on its basis. A promising area of application of this concentrate will be the prevention and treatment of gastric ulcer
{"title":"Research of antiulcer activity of dietary concentrate of phenolic compounds of apples on models of experimental ulcer in rats.","authors":"L. Galuzinska, V. Kravchenko, G. Kravchenko, T. Briukhanova, V.Р. Fylymonenko","doi":"10.15587/2519-8025.2021.241538","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.241538","url":null,"abstract":"Medicinal plants have been widely used in the treatment of many diseases, especially in the gastroenterology, given that the use of synthetic antiulcer drugs in clinical practice often leads to the development of various kinds of side effects and, accordingly, have a large list of contraindications. The aim of this experimental work was the study of the antiulcer activity of the food concentrate of phenolic compounds of apples on the models of experimental ulcers in rats. Materials and methods. The studies included two stages, which were carried out on models: stage I – a model of alcohol–prednisolone stomach ulcer in rats, stage II – a model of indomethacin stomach ulcer in rats in accordance with the methodological recommendations of the SEC of the Ministry of Health of Ukraine. The studied concentrate was administered in a dose based on the total content of polyphenols – 9 mg per 100 g of weight. Quercetin at a dose of 5 mg / kg and ranitidine at a dose of 20 mg / kg were used as reference drugs. After replicating the models, a macroscopic examination of the gastric mucosa was carried out and the content of TBA-reactants and reduced glutathione in the stomach homogenate was determined. Results. Under pathological conditions, the therapeutic effect of the food concentrate of phenolic compounds of apples and reference drugs was comparable in terms of the degree of ulcer defect. It was proved that food concentrate of phenolic compounds of apples and reference drugs inhibit the course of lipid peroxidation reactions and support endogenous antioxidant defense systems. Conclusions. The obtained results make it possible to recommend a further study of the food concentrate of phenolic compounds of apples for the creation of a pharmaceutical preparation on its basis. A promising area of application of this concentrate will be the prevention and treatment of gastric ulcer","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"183 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74620151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15587/2519-8025.2021.241207
A. Zemlianskyi, O. Tymoshenko, A. Zakhariev, Yevheniia Vashchyk, N. Seliukova, R. Dotsenko
The aim of the research: to substantiate the pathogenetic role of lipid metabolism disorders in the occurrence and course of pancreatitis in dogs and to establish the informativeness of its indicators for diagnosis and evaluation of treatment effectiveness based on clinical and laboratory research methods. Materials and methods. Used clinical, instrumental, laboratory methods, including morphological studies of blood, urine, biochemical studies of serum: determination of the lipid profile by the content of triacylglycerols, total cholesterol, lipoproteins cholesterol of very low – HDL, low – LDL and high density – VLDL, traditional tests and statistical methods. Results. It was found that the most significant changes are inherent in the metabolism of lipids and lipoproteins in the serum: significantly increased concentrations of cholesterol, triacylglycerols, LDL cholesterol and especially LDL cholesterol. The content of HDL cholesterol, on the contrary, in the acute course of pancreatitis is below normal. The content of triacylglycerols, LDL cholesterol and VLDL remains high. Total cholesterol is normalized, and the level of HDL is below normal. Conclusions. Serum lipidogram of dogs without clinical symptoms of pathology does not differ in composition from the results obtained by other researchers. For pancreatitis in dogs, the concentration of total cholesterol increases by 1.9 times, triacylglycerols – by 5.4 times, cholesterol VLDL – in 6.0 times and LDL cholesterol – 7.7 times. The content of HDL cholesterol, in contrast, in the acute course of pancreatitis is 1.5 times lower than in clinically healthy dogs. Treatment reduces the degree of hyperenzymemia by ALT and AST, but α-amylase activity is not normalized in dogs. Above normal levels of triacylglycerols, LDL cholesterol and VLDL, and HDL cholesterol remain low, despite treatment
{"title":"Informativity of lipid metabolism indicators for diagnostics and estimation of the effectiveness of treatment of dogs with pancreatitis","authors":"A. Zemlianskyi, O. Tymoshenko, A. Zakhariev, Yevheniia Vashchyk, N. Seliukova, R. Dotsenko","doi":"10.15587/2519-8025.2021.241207","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.241207","url":null,"abstract":"The aim of the research: to substantiate the pathogenetic role of lipid metabolism disorders in the occurrence and course of pancreatitis in dogs and to establish the informativeness of its indicators for diagnosis and evaluation of treatment effectiveness based on clinical and laboratory research methods. Materials and methods. Used clinical, instrumental, laboratory methods, including morphological studies of blood, urine, biochemical studies of serum: determination of the lipid profile by the content of triacylglycerols, total cholesterol, lipoproteins cholesterol of very low – HDL, low – LDL and high density – VLDL, traditional tests and statistical methods. Results. It was found that the most significant changes are inherent in the metabolism of lipids and lipoproteins in the serum: significantly increased concentrations of cholesterol, triacylglycerols, LDL cholesterol and especially LDL cholesterol. The content of HDL cholesterol, on the contrary, in the acute course of pancreatitis is below normal. The content of triacylglycerols, LDL cholesterol and VLDL remains high. Total cholesterol is normalized, and the level of HDL is below normal. Conclusions. Serum lipidogram of dogs without clinical symptoms of pathology does not differ in composition from the results obtained by other researchers. For pancreatitis in dogs, the concentration of total cholesterol increases by 1.9 times, triacylglycerols – by 5.4 times, cholesterol VLDL – in 6.0 times and LDL cholesterol – 7.7 times. The content of HDL cholesterol, in contrast, in the acute course of pancreatitis is 1.5 times lower than in clinically healthy dogs. Treatment reduces the degree of hyperenzymemia by ALT and AST, but α-amylase activity is not normalized in dogs. Above normal levels of triacylglycerols, LDL cholesterol and VLDL, and HDL cholesterol remain low, despite treatment","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85018143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.15587/2519-8025.2021.241238
Yevheniia Vashchyk, D. Morozenko, N. Seliukova, A. Zakhariev, R. Dotsenko, A. Zemlianskyi, O. Shapovalova, E. Dotsenko
The aim: the aim of the research is an analytical review of the scientific literature on Рseudomonas aeruginosa as a priority group representative of bacteria with multiple antibiotics resistance. Materials and methods. The research was conducted by the method of scientific literature open source analysis: PubMed, Elsevier, electronic resources of the National Library named after V. I. Vernadsky and others. Results. The problem of antibiotic resistance is rightly called the “apocalypse of the XXI century”. P. aeruginosa bacteria are characterized by a very high natural ability to form resistant forms to antimicrobial drugs due to the formation of specific resistance genes, the ability to resist the entry of antibiotics into the cell or remove antibiotics from the cell, and form biofilms. A characteristic feature of the epidemic and epizootic processes of P. aeruginosa, as an opportunistic ubiquitous microorganism is host-pathogenic interaction - the interaction of the pathogen with the host organism. The peculiarity of this bacterium is opportunism and long-term persistence in the body of the host and in the environment. The global trend towards the spread of antibiotic-resistant gram-negative bacteria, including P. aeruginosa, underscores the need to develop comprehensive response strategies targeting all sectors of health. Conclusions. Bacteria P. aeruginosa is classified in 1th Critical Group of the WHO list of resistant to antibiotics "priority pathogens". Among the main reasons that contribute to the emergence of resistance are irrational antibiotic therapy in both humans and animals, and the use of antibiotics as growth stimulants in animal husbandry. While more R&D is vital, alone, it cannot solve the problem. To address resistance, there must also be better prevention of infections and appropriate use of existing antibiotics in humans and animals, as well as rational use of any new antibiotics that are developed in future. New methods of combating antibiotic resistance and antibacterial substances, alternatives to antibiotics (biofilm-destroying drugs, antimicrobial peptides (AMP), bacteriophages, nanopreparations, etc.) can make a positive contribution to overcoming the multiple drug resistance of gram-negatives
目的:本研究的目的是对Рseudomonas铜绿假单胞菌作为多种抗生素耐药细菌的优先组代表的科学文献进行分析综述。材料和方法。本研究采用科学文献开源分析的方法:PubMed, Elsevier，以V. I. Vernadsky命名的国家图书馆电子资源等。结果。抗生素耐药性问题被恰当地称为“21世纪的末日”。铜绿假单胞菌的特点是，由于形成特定的耐药基因，能够抵抗抗生素进入细胞或将抗生素从细胞中清除，并形成生物膜，因此对抗菌药物形成耐药形式的天然能力非常高。铜绿假单胞菌作为一种机会性普遍存在的微生物，其流行和动物流行病过程的一个特征是宿主-致病性相互作用，即病原体与宿主生物的相互作用。这种细菌的特点是机会主义和长期存在于宿主体内和环境中。包括铜绿假单胞菌在内的耐抗生素革兰氏阴性细菌在全球蔓延的趋势突出表明，需要制定针对所有卫生部门的综合应对战略。结论。铜绿假单胞菌被列为世卫组织抗生素耐药“重点病原体”清单的第11类。导致耐药性出现的主要原因是人类和动物不合理的抗生素治疗，以及在畜牧业中使用抗生素作为生长刺激剂。虽然更多的研发是至关重要的，但单靠研发并不能解决问题。为了解决耐药性问题，还必须更好地预防感染，在人类和动物中适当使用现有抗生素，以及合理使用未来开发的任何新抗生素。对抗抗生素耐药性和抗菌物质的新方法、抗生素替代品(破坏生物膜的药物、抗菌肽(AMP)、噬菌体、纳米修复物等)可以为克服革兰氏阴性菌的多重耐药性做出积极贡献
{"title":"Pseudomonas aeruginosa as a priority group representative of bacteria with multiple antibiotic resistance","authors":"Yevheniia Vashchyk, D. Morozenko, N. Seliukova, A. Zakhariev, R. Dotsenko, A. Zemlianskyi, O. Shapovalova, E. Dotsenko","doi":"10.15587/2519-8025.2021.241238","DOIUrl":"https://doi.org/10.15587/2519-8025.2021.241238","url":null,"abstract":"The aim: the aim of the research is an analytical review of the scientific literature on Рseudomonas aeruginosa as a priority group representative of bacteria with multiple antibiotics resistance. Materials and methods. The research was conducted by the method of scientific literature open source analysis: PubMed, Elsevier, electronic resources of the National Library named after V. I. Vernadsky and others. Results. The problem of antibiotic resistance is rightly called the “apocalypse of the XXI century”. P. aeruginosa bacteria are characterized by a very high natural ability to form resistant forms to antimicrobial drugs due to the formation of specific resistance genes, the ability to resist the entry of antibiotics into the cell or remove antibiotics from the cell, and form biofilms. A characteristic feature of the epidemic and epizootic processes of P. aeruginosa, as an opportunistic ubiquitous microorganism is host-pathogenic interaction - the interaction of the pathogen with the host organism. The peculiarity of this bacterium is opportunism and long-term persistence in the body of the host and in the environment. The global trend towards the spread of antibiotic-resistant gram-negative bacteria, including P. aeruginosa, underscores the need to develop comprehensive response strategies targeting all sectors of health. Conclusions. Bacteria P. aeruginosa is classified in 1th Critical Group of the WHO list of resistant to antibiotics \"priority pathogens\". Among the main reasons that contribute to the emergence of resistance are irrational antibiotic therapy in both humans and animals, and the use of antibiotics as growth stimulants in animal husbandry. While more R&D is vital, alone, it cannot solve the problem. To address resistance, there must also be better prevention of infections and appropriate use of existing antibiotics in humans and animals, as well as rational use of any new antibiotics that are developed in future. New methods of combating antibiotic resistance and antibacterial substances, alternatives to antibiotics (biofilm-destroying drugs, antimicrobial peptides (AMP), bacteriophages, nanopreparations, etc.) can make a positive contribution to overcoming the multiple drug resistance of gram-negatives","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91093529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-30DOI: 10.15587/2519-8025.2020.217554
N. Shtemenko, K. Polokhina, A. Golichenko, S. Babiy, A. Shtemenko
The aim of the study. The aim of the work was to investigate in vivo anticancer activity of cis- and trans-diadamanthylcarboxylates of dirhenium(III) alone and together with cisplatin in form of nanobins.Materials and methods. Model of tumor growth, Guerin’s carcinoma; intraperitoneal administration of cisplatin, dirhenium(III) compounds in liposomes and of binary liposomes, containing both cytostatics; volumes and final weights of tumors were measured.Results. In vivo antitumor properties of two dirhenium(III) dicarboxylates with 1-adamantanecarboxylic acid moieties as ligands with cis- (I) and trans- (II) orientation of the carboxylic groups around a cluster fragment alone and together with cisplatin were presented; an attempt to understand differences in a possible mechanism of anticancer activity of the substances were undertaken. Antiradical and DNA-binding properties of I and II were the matter of consideration.Conclusions. Cis- and trans- compounds of dirhenium I and II had close antitumor activity in vivo with a little bit superiority of the cis- analog. Mechanisms of anticancer activity of I and II are different and may also include monofunctional adduct formation and subsequent interstrand cross-linking for the II substance, formation of protein-DNA cross-links, etc.
{"title":"Determination of the anticancer properties of cis- and trans-diadamanthylcarboxylates of dirhenium(III)","authors":"N. Shtemenko, K. Polokhina, A. Golichenko, S. Babiy, A. Shtemenko","doi":"10.15587/2519-8025.2020.217554","DOIUrl":"https://doi.org/10.15587/2519-8025.2020.217554","url":null,"abstract":"The aim of the study. The aim of the work was to investigate in vivo anticancer activity of cis- and trans-diadamanthylcarboxylates of dirhenium(III) alone and together with cisplatin in form of nanobins.Materials and methods. Model of tumor growth, Guerin’s carcinoma; intraperitoneal administration of cisplatin, dirhenium(III) compounds in liposomes and of binary liposomes, containing both cytostatics; volumes and final weights of tumors were measured.Results. In vivo antitumor properties of two dirhenium(III) dicarboxylates with 1-adamantanecarboxylic acid moieties as ligands with cis- (I) and trans- (II) orientation of the carboxylic groups around a cluster fragment alone and together with cisplatin were presented; an attempt to understand differences in a possible mechanism of anticancer activity of the substances were undertaken. Antiradical and DNA-binding properties of I and II were the matter of consideration.Conclusions. Cis- and trans- compounds of dirhenium I and II had close antitumor activity in vivo with a little bit superiority of the cis- analog. Mechanisms of anticancer activity of I and II are different and may also include monofunctional adduct formation and subsequent interstrand cross-linking for the II substance, formation of protein-DNA cross-links, etc.","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81199507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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