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Analysis of phyto-constituents, antioxidant and antidiabetic activities of some medicinal plants of Nepal 尼泊尔部分药用植物成分及抗氧化、抗糖尿病活性分析
Pub Date : 2022-06-14 DOI: 10.3126/sw.v15i15.45645
Bimala Subba, Sanjay Gaire
The plant kingdom is a wide field to search for natural effective antioxidant and oral hypoglycaemic agents that have slight or no side effects. The present study was carried out to analyze the phytochemical, antioxidant and anti-diabetic activities of six ethno-medical plants of Nepal viz Berberis aristata, Murraya koenigii, Xanthium strumarium, Cuscuta reflexa, Myrica esculenta and Syzygium cumini. Phytochemical screening of methanolic extract of all selected plants showed the presence of different chemical constituents such as alkaloids, polyphenols, flavonoids, terpenoids, saponins, glycosides and tanins. The antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity. Ascorbic acid was used as standard during DPPH assay. S. cumini and M. esculenta were seen most active with IC50 values 33.35 µg/mL, 35.19 µg/mL, respectively. The anti-diabetic activity was evaluated by using α-amylase inhibition method. Acarbose was used as positive control during the assay. X. strumarium, S. cumini, M. esculenta, B. aristata were found to be potent towards α-amylase inhibition with IC50 values 197.21 µg/mL, 206.22 µg/mL, 246.07 µg/mL, 270.04 µg/mL.
植物界是一个广泛的领域,寻找天然有效的抗氧化剂和口服降糖药,有轻微或没有副作用。对尼泊尔6种民族药用植物小檗(Berberis aristata)、木犀草(Murraya koenigii)、苍耳草(Xanthium strumarium)、木犀草(Cuscuta reflexa)、杨梅(Myrica esculenta)和小茴香(Syzygium cumini)的化学成分、抗氧化和抗糖尿病活性进行了研究。对所选植物的甲醇提取物进行植物化学筛选,发现其含有生物碱、多酚、黄酮类、萜类、皂苷、糖苷和单宁等不同的化学成分。以2,2 -二苯基-1-苦基酰肼(DPPH)自由基清除能力评价其抗氧化活性。DPPH测定以抗坏血酸为标准品。cumini和M. esculenta活性最强,IC50值分别为33.35µg/mL和35.19µg/mL。采用α-淀粉酶抑制法评价其抗糖尿病活性。以阿卡波糖为阳性对照。对α-淀粉酶有较强的抑制作用,IC50值分别为197.21µg/mL、206.22µg/mL、246.07µg/mL、270.04µg/mL。
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引用次数: 0
Assessment of hydrochemical characteristics of the Taudaha lake, Kathmandu, Nepal 尼泊尔加德满都陶达哈湖水化学特征评价
Pub Date : 2022-06-14 DOI: 10.3126/sw.v15i15.45651
R. Pant, Ganga Paudel, Bindu Dahal, K. Bishwakarma, Sanjeeb Sharma, S. Shrestha, M. L. Sharma, Mahesh Prasad Awasthi
Taudaha Lake is an important lake in central Nepal because of its cultural, aesthetic, ecological and economic values. The assessments of water quality and hydrochemical characteristics were carried out by collecting samples from 20 different sites of the lake in August 2021. Analysis of temperature, pH, electrical conductivity, total dissolved solids, turbidity, dissolved oxygen, total hardness, major cations (Ca2+, Mg2+, Na+, K+, Fe3+ and NH4+) and major anions (HCO3-, Cl-, NO3-, SO42- and PO43-) were carried out to assess the overall hydrochemistry of the lake. The obtained data were interpreted by using multivariate statistical techniques to explore pollution sources and characteristics of sampling points and compared with WHO and irrigation guidelines. Slightly alkaline water with less distinct spatial variation was found. The pattern of major ions is in order of Ca2+> Mg2+> Na+> K+> Fe3+> NH4+ and HCO3-> Cl-> SO42-> NO3-> PO43-.  All parameters lie within the limit of WHO guidelines. Principal component analysis (PCA) provides four major components with 73.06% cumulative variance and hierarchical cluster analysis classifies five clusters. Results of PCA, CA, Piper, Gibbs, and mixing plots suggested that the hydrochemistry of the lake is primarily controlled by underlying carbonate-dominate lithology. Similarly, Wilcox diagram suggests that lake water is not severely affected by anthropic pollution and is suitable for irrigational purposes. It is recommended for further study on seasonal, and depth-wise water quality variations along with socio-ecological and ecotourism perspectives in the future.
陶达哈湖因其文化、美学、生态和经济价值而成为尼泊尔中部的一个重要湖泊。2021年8月,通过收集湖泊20个不同地点的样本,对水质和水化学特征进行了评估。通过分析温度、pH、电导率、总溶解固形物、浊度、溶解氧、总硬度、主要阳离子(Ca2+、Mg2+、Na+、K+、Fe3+和NH4+)和主要阴离子(HCO3-、Cl-、NO3-、SO42-和PO43-)来评价湖泊的整体水化学。利用多元统计技术对获得的数据进行解释,以探索污染源和采样点的特征,并与世卫组织和灌溉指南进行比较。微碱性水空间变化不明显。主要离子的排列顺序为Ca2+> Mg2+> Na+> K+> Fe3+> NH4+和HCO3-> Cl-> SO42-> NO3-> PO43-。所有参数都在世卫组织准则的限制范围内。主成分分析(PCA)提供了4个主成分,累积方差为73.06%,层次聚类分析分类出5个聚类。PCA图、CA图、Piper图、Gibbs图和混合图结果表明,该湖的水化学主要受下伏碳酸盐岩岩性控制。同样,Wilcox图表明,湖水没有受到人为污染的严重影响,适合用于灌溉目的。建议在未来进一步研究季节和深度水质变化以及社会生态和生态旅游的观点。
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引用次数: 2
Study of substructure analysis of supercluster S [227+006+0078] radial velocity range of 21300 km/s to 23400 km/s 超星团S[227+006+0078]径向速度范围为21300 ~ 23400 km/ S的子结构分析研究
Pub Date : 2022-06-14 DOI: 10.3126/sw.v15i15.45667
J. Malla, W. Saurer, B. Aryal
This paper presents the search for substructures within the Supercluster S [227+006+0078]. To use the spectroscopic database (7th data release) of galaxies, studied the number density, all-sky distribution, and redshift maps to identify substructures based on their richness and compactness. And also to find out the substructures within the Supercluster, using the contour plot of number densities of galaxies within the appropriate radius values and number of nearby galaxies.
本文介绍了在超星系团S[227+006+0078]内寻找子结构的方法。利用星系光谱数据库(第七次数据发布),研究了星系的数量密度、全天空分布和红移图,根据它们的丰富度和紧凑度来识别子结构。并利用适当半径范围内的星系数量密度和附近星系数量的等高线图,找出超星系团内部的子结构。
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引用次数: 0
Quality appraisal of drinking water from different sources in Nepal 尼泊尔不同来源的饮用水质量评价
Pub Date : 2022-06-14 DOI: 10.3126/sw.v15i15.45656
T. Prasai Joshi, Saraswati Gaihre, Sujata Dhungel, S. Acharya, Samikshya Kandel, Naina Byanjankar
This research was investigated to evaluate the quality assurance of water from different sources. All together 250 water samples (135 well water, 48 boring water, 50 treated water, and 17 tap water) were received from different parts of Nepal from December 2019 to April 2020. The physicochemical parameters of water samples were performed according to the standard methods for the examination of water and wastewater. The membrane filtration technique was applied for the determination of Total Coliform bacteria. The measurements of water quality parameters were compared with the upper and lower limits of the National Drinking Water Quality Standards (NDWQS), 2005. Out of 135 well water samples, pH (1.48%), conductivity (2.22%), turbidity (42.96%), total hardness (4.44%), iron (54.07%), ammonia (48.88%), and nitrate (2.22%) elevated values compared to National Drinking Water Quality Standards, 2005. Likewise, 2.08%, 6.25%, 64.58%, 4.1%, 47.91%, and 58.33% of boring water samples showed higher values than the National Drinking Water Quality Standards for pH, conductivity, turbidity, total hardness, iron, ammonia, respectively. Conductivity, total hardness, chloride, and iron were found below the standards for both treated and tap water samples. Arsenic concentration was found within the standard for all water samples while 0.74% of well water samples showed a higher concentration of chloride compared to the standard. Results revealed that the minimum and maximum concentrations of some parameters were found to vary among the water sources. Among the total water samples, 94.8% well water, 76.4% tap water, 56.0% treated water, and 14.6% boring water samples showed the presence of coliform bacteria. This concludes that most of the water sources were polluted with fecal contamination and without proper purification may lead to the risk of waterborne diseases. Therefore, systematic and regular monitoring of water sources should be implemented to maintain water quality.
本研究对不同水源的水质保证进行了研究。从2019年12月至2020年4月,共从尼泊尔不同地区收集了250个水样(135个井水、48个钻孔水、50个处理水和17个自来水)。按水、废水检验标准方法测定水样的理化参数。采用膜过滤法测定总大肠菌群。将水质参数测量值与2005年国家饮用水质量标准(NDWQS)的上下限进行比较。135份井水样品的pH值(1.48%)、电导率(2.22%)、浊度(42.96%)、总硬度(4.44%)、铁(54.07%)、氨(48.88%)和硝酸盐(2.22%)高于2005年《国家饮用水质量标准》。钻孔水样pH、电导率、浊度、总硬度、铁、氨高于国家饮用水水质标准的比例分别为2.08%、6.25%、64.58%、4.1%、47.91%和58.33%。电导率、总硬度、氯化物和铁都低于处理过的和自来水样品的标准。所有水样的砷含量均在标准范围内,而0.74%的井水氯化物含量高于标准。结果表明,某些参数的最小和最大浓度在不同的水源之间存在差异。在全部水样中,94.8%的井水、76.4%的自来水、56.0%的处理水和14.6%的钻孔水样存在大肠菌群。结论是,大部分水源受到粪便污染,未经适当净化可能导致水传播疾病的风险。因此,应对水源进行系统和定期的监测,以保持水质。
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引用次数: 4
A brief review on the solutions of advection-diffusion equation 平流扩散方程解的简要评述
Pub Date : 2022-06-14 DOI: 10.3126/sw.v15i15.45668
Pushpa Gautam, B. P. Sapkota, K. N. Uprety
In this work both linear and nonlinear advection-diffusion equations are considered and discussed their analytical solutions with different initial and boundary conditions. The work of Ogata and Banks, Harleman and Rumer, Cleary and Adrian, Atul Kumar et al., Mojtabi and Deville are reviewed for linear advection-diffusion equations and for nonlinear, we have chosen the work of Sakai and Kimura. Some enthusiastic functions used in the articles, drawbacks and applications of the results are discussed. Reduction of the advection-diffusion equations into diffusion equations make the governing equation solvable by using integral transform method for analytical solution. For nonlinear advection-diffusion equations, the Cole-Hopf transformation is used to reduce into the diffusion equation. Different dispersion phenomena in atmosphere, surface and subsurface area are outlined.
本文讨论了线性和非线性平流扩散方程在不同初始条件和边界条件下的解析解。对Ogata和Banks、Harleman和Rumer、Cleary和Adrian、Atul Kumar等人、Mojtabi和Deville的线性平流扩散方程和非线性平流扩散方程的研究进行了回顾,我们选择了Sakai和Kimura的研究。讨论了文章中使用的一些热情函数、缺点和结果的应用。将平流扩散方程化为扩散方程,使控制方程可以用解析解的积分变换方法求解。对于非线性平流扩散方程,采用Cole-Hopf变换将其约化为扩散方程。概述了大气、地表和地下的不同色散现象。
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引用次数: 0
Further studying the Dirac field in FLRW space FLRW空间中狄拉克场的进一步研究
Pub Date : 2022-06-14 DOI: 10.3126/sw.v15i15.45632
P. Dhungel, S. Sharma, U. Khanal
Behaviour of the Dirac field in FLRW space is further investigated extending the previous work. WKB approximation up to the third order was used to solve the temporal part of the Dirac’s equation and hence the energy and particle current have been calculated to further details.
在此基础上,进一步研究了FLRW空间中狄拉克场的行为。利用三阶WKB近似求解了狄拉克方程的时间部分,从而进一步计算了能量和粒子电流。
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引用次数: 0
Assessment of Air Pollution Impact on Micro-Morphological and Biochemical Properties Of Callistemon citrinus (Curtis) Skeels And Lagerstroemia indica L. 空气污染对桔梗和紫薇微形态及生化特性影响的评价
Pub Date : 2021-02-17 DOI: 10.3126/SW.V14I14.35024
S. Shrestha, A. Devkota, P. K. Jha
The urban air pollution is a major environmental concern, particularly in the developing countries and in their major cities. In the present study an attempt has been made to assess the air pollution effect on micromorphological and biochemical parameters of Callistemon citrinus and Lagerstroemia indica. Biochemical parameters were analyzed by using standard protocol. one-way analysis of variance was performed with SPSS software, v. 20.0, and Duncan test (p ≤ 0.05) was used to compare averages of all measured biochemical parameters and micromorpholgical features. There was a decrease in number of stomata of both species at highly polluted site compared to less polluted site (control). Stomatal index of both species was found to be reduced in polluted site when compared to control. The number of clogged stomata was less in control area samples when compared to polluted sample. A number of epidermal cells were significantly decreased at heavily polluted site. Total chlorophyll content and leaf extract pH in both the plants were found to be significantly higher in control sites than in heavily polluted sites. Ascorbic acid, relative water content, and Air Pollution Tolerance Index (ATPI) was found to be significantly higher at more polluted plants than at control. Based on the present study results , Callistemon citrinus emerged as the most tolerant species with the highest APTI. Hence, Callistemon citrinus can be suggested for plantations along the roadside of urban areas for green belt development.
城市空气污染是一个主要的环境问题,特别是在发展中国家及其主要城市。本文研究了空气污染对柑桔花萼和紫薇微形态和生化指标的影响。采用标准方案分析生化参数。采用SPSS软件(v. 20.0)进行单因素方差分析,采用Duncan检验(p≤0.05)比较各测定生化参数和微形态特征的平均值。两种植物在高污染样地的气孔数量均低于低污染样地(对照)。两种植物在污染场地的气孔指数均低于对照。与污染样品相比,控制区样品中气孔堵塞的数量较少。重污染部位表皮细胞数量明显减少。两种植物的总叶绿素含量和叶提取物pH值在对照区显著高于重污染区。抗坏血酸、相对含水量和空气污染耐受指数(ATPI)均显著高于对照。基于目前的研究结果,Callistemon citrinus是最耐受性最强的品种,APTI最高。因此,可以建议在市区的路边种植柑橘,进行绿化带的开发。
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引用次数: 5
Biosorptio Of Cd2+ By Wildtype And Cadmium Resistant, CdR-99 Cell Line Of Chlorella Vulgaris 普通小球藻野生型和抗镉型CdR-99细胞系对Cd2+的生物吸附
Pub Date : 2021-02-17 DOI: 10.3126/SW.V14I14.35022
Sudarshana Shakya, Bindra Devi Shakya, A. Tamrakar, B. Adhikari, Sabita Nepal, P. Shakya
Removal of heavy metals is very important in wastewater treatment process, due to their toxic effects on the environment. Biological treatment has attracted researchers for years since it has many advantages over physical and chemical methods for removing heavy metals from wastewater. The purpose of this research was to assess the biosorption of Cd2+ by wildtype (WT) and CdR-99 resistant line of Chlorella vulgaris confirming mechanisms of resistance to Cd2+ toxicity and the effect of the variable concentrations of Cd2+ on their growth. Exposure of both algal cell lines to increasing Cd2+ concentrations resulted in progressive inhibition of growth as revealed by growth experiments. The higher ID50 value (38 M Cd2+) of CdR-99 resistant line exhibited some degree of resistance to Cd2+ toxicity. Metal content was determined by flame atomic absorption spectrometry (FAAS). When exposed to the growth medium containing 50 M Cd2+, CdR-99 resistant isolate proved to be efficient cell line compared to the WT, in terms of adsorption and removal of Cd2+ at 15 min and 48 hr interval of time respectively. Extracellular Cd2+ adsorption was found significantly higher than intracellular uptake in both the tested cell lines. Total Cd2+ accumulation and distribution between the external and internal cell fractions of the CdR-99 were significantly higher to the WT. Thus, the CdR-99 cell line appeared more resistant to Cd2+ toxicity and hence may be used for wastewater treatment and remediation of metal contaminated sites.
由于重金属对环境的毒性作用,重金属的去除在废水处理过程中非常重要。由于生物处理在去除废水中的重金属方面比物理和化学方法有许多优点,多年来一直吸引着研究人员的注意。研究了野生型(WT)和CdR-99抗性小球藻(Chlorella vulgaris)对Cd2+的生物吸附,确定了小球藻对Cd2+毒性的抗性机制以及不同浓度的Cd2+对其生长的影响。生长实验显示,两种藻类细胞系暴露于Cd2+浓度增加的环境中,会导致生长的进行性抑制。cddr -99抗性品系的ID50值较高(38M Cd2+),对Cd2+毒性表现出一定程度的抗性。采用火焰原子吸收光谱法(FAAS)测定金属含量。当暴露于含有50M Cd2+的生长培养基时,CdR-99抗性分离株在吸附和去除Cd2+的时间间隔分别为15 min和48 hr,与WT相比,CdR-99抗性分离株在Cd2+的吸附和去除方面表现得更好。细胞外Cd2+吸附明显高于细胞内摄取。Cd2+在CdR-99细胞内外组分之间的累积和分布明显高于WT,因此CdR-99细胞系对Cd2+的毒性表现出更强的抗性,因此可以用于废水处理和金属污染场地的修复。
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引用次数: 1
Study Of Tremulous Signatures In Devanagari Nepali Script 尼泊尔梵文文字震颤特征研究
Pub Date : 2021-02-17 DOI: 10.3126/SW.V14I14.35020
M. Pradhan, M. Bajracharya, Manisha Rai, Piyush Shakya, Basudev Kc, B. Bhusal
Signatures, as one of the behavioral human characteristics are generally recognized as legal means for verifying an individual's identity by administrative and financial institutions. A signature is developed by a person and it can change over time. Signatures are prone to influences by age, physical and mental conditions. In this study tremulous signatures including questioned and standards related to 50 real cases resolved by National Forensic Science Laboratory of Nepal were collected. The time interval between questioned and standard signatures was up to ten years. Sixteen major characteristics along with their forty-one sub characteristics of each case were studied. Among sixteen major characteristics in the tremulous signatures, study shows that the characteristics like the style of writing in hand printed form, pen pauses, medium pen pressure, poor line quality, pen lifts are most prominently observed. Tremors were absent mostly in the terminal strokes of letters. The letters written were varied in size and those were 6% small, 70% medium and 24% large. Interestingly no any retouch attempts were found to complete the shape of letters.
签名作为人的行为特征之一,被行政和金融机构普遍认为是验证个人身份的合法手段。一个签名是由一个人形成的,它可以随着时间的推移而改变。签名容易受到年龄、身体和精神状况的影响。本研究收集了尼泊尔国家法医学实验室解决的50个真实案例的震颤签名,包括质疑和标准。被质疑的签名和标准签名之间的时间间隔长达十年。对每个病例的16个主要特征及其41个次要特征进行了研究。研究表明,在颤音签名的16个主要特征中,手印写法、笔停顿、笔压适中、线条质量差、笔举等特征最为突出。在字母的最后一划中没有震颤。所写的字母大小不一,其中6%为小,70%为中,24%为大。有趣的是,没有任何修饰尝试被发现来完成字母的形状。
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引用次数: 0
Characterizations Of Water Quality In West-Seti and Tamor River Basins, Nepal 尼泊尔西塞提河和塔莫尔河流域水质特征
Pub Date : 2021-02-17 DOI: 10.3126/SW.V14I14.35021
N. Ghimire, N. Adhikari, R. Pant, S. Thakuri
This study presents the geochemical composition and water quality of waters in the West-Seti and Tamor River basins in Nepal Himalaya with the aim to reveal their hydrochemical characteristics and to evaluate the water quality. Water samples were collected from 18 sites of the rivers in the pre-monsoon season and analysed the physicochemical parameters to characterize their quality. The parameterstemperature, pH, conductivity, and total dissolved solids were measured in the field, while the major ions (Na, Ca, Mg, Si, SO4, NO3, HCO3, Cl, and F) were analyzed in the laboratory. Overall results of physicochemical parameters revealed that the ionic strength is much distinct; however, the waters are chemically pure in both the river basins with very less electrical conductivity (<250 μS/cm) and total dissolved solids (<120 mg/L). Bicarbonate (HCO3) has a significant correlation with Ca and Mg suggesting carbonate rock weathering as the dominant geochemical process in both of the basins. The concentrations of Ca, Mg, and HCO3 in the water of the West-Seti is relatively higher than the waters in the Tamor River basin. Mostly, the geochemical facies of both the rivers are characterized by the Ca-Mg-HCO3type (88.9%), with dominant carbonate dominated lithology. However, hydrochemical facies clearly suggested spatial discrimination between two basins with dominant geogenic signatures as Ca-SO4-Cl type water facies are also reported from the Tamor River basin. The results exhibited that the concentrations of measured parameters were relatively very low and within the WHO guideline values and currently under a safe level of the water quality for drinking and ecosystem health perspectives; however, further in-depth research is recommended in the periodic basis to assess traces of climate change imprints, and anthropogenic interferences for more consistent and reliable dataset. The findings of this study could be useful for the water quality management in the glacier-fed Himalayan River basins.
本文对尼泊尔-喜马拉雅地区西塞提河和塔莫尔河流域的水化学组成和水质进行了研究,旨在揭示其水化学特征,并对水质进行评价。在季风来临前,从18个地点采集了水样,并分析了水质的理化参数。现场测量了温度、pH、电导率和总溶解固形物等参数,实验室分析了主要离子(Na、Ca、Mg、Si、SO4、NO3、HCO3、Cl和F)。理化参数的综合结果表明,离子强度差异很大;然而,这两个流域的水都是化学纯净的,电导率(<250 μS/cm)和总溶解固形物(<120 mg/L)都很低。碳酸氢盐(HCO3)与Ca、Mg具有显著的相关性,表明碳酸盐岩风化是两个盆地主要的地球化学过程。西塞提地区水体中Ca、Mg和HCO3的浓度相对高于塔莫尔河流域。两河地球化学相以ca - mg - hco3型(88.9%)为主,岩性以碳酸盐为主。而水化学相则明确显示了两个盆地之间的空间区别,具有明显的地质特征,如塔莫尔河流域的Ca-SO4-Cl型水相。结果表明,所测参数的浓度相对较低,在世卫组织指导值范围内,目前处于饮用水和生态系统健康方面的安全水质水平之下;然而,建议在定期基础上进一步深入研究,以评估气候变化印记和人为干扰的痕迹,以获得更一致和可靠的数据集。本研究结果对喜马拉雅冰川流域的水质管理具有一定的指导意义。
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引用次数: 4
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