Pub Date : 2023-06-05DOI: 10.17268/sci.agropecu.2023.021
Samuel Julca-Infantes, J. P. Cruz‐Tirado, Delia Izaguirre-Torres
El uso de herramientas tecnológicas, en adición a las técnicas tradicionales, para conocer las emociones y preferencia de los consumidores a productos alimenticios es muy importante para que los desarrolladores de productos tengan mejor información para la toma de decisiones. El objetivo del presente estudio fue utilizar herramientas de la neurogastronomía y neuromarketing para estudiar las emociones y preferencias visuales de los consumidores hacia hojuelas de papas (Solanum tuberosum) nativas y clásicas. Se utilizaron dos tipos de hojuelas frita, papa clásica y papa nativa, con un costo de S/ 6,60 (1.79 USD) y S/ 7,00 (1.90 USD), respectivamente. En una primera etapa se utilizó la tecnología de Seguimiento Ocular y el Análisis de Emoción utilizando una aplicación para teléfono móvil. En una segunda etapa, cada participante respondió una encuesta cognitiva, mediante el uso de formulario Google. Se utilizaron 100 consumidores con edades entre 14 y 63 años. La encuesta mostró que un 45% de los consumidores mostraron su intención de comprar el producto nativo más que el producto clásico. Los resultados de neuromarketing mostraron principalmente una mayor proporción de neutralidad, y un poco menor, en emociones negativas de “escepticismo”, “tristeza” y “disgusto”, especialmente sobre el factor nutricional y el precio. Estos resultados, más que contradictorios son complementarios, ya que las herramientas tecnológicas nos permiten conocer más detalles de las preferencias y elecciones. Los consumidores pretenden consumir más productos nativos, siempre que sean más nutritivos, más sustentables, más baratos y beneficien al productor local.
{"title":"Emotions and visual preferences of consumers towards native and classic potato (Solanum tuberosum) chips: Comparative study using neurogastronomy and neuromarketing","authors":"Samuel Julca-Infantes, J. P. Cruz‐Tirado, Delia Izaguirre-Torres","doi":"10.17268/sci.agropecu.2023.021","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.021","url":null,"abstract":"El uso de herramientas tecnológicas, en adición a las técnicas tradicionales, para conocer las emociones y preferencia de los consumidores a productos alimenticios es muy importante para que los desarrolladores de productos tengan mejor información para la toma de decisiones. El objetivo del presente estudio fue utilizar herramientas de la neurogastronomía y neuromarketing para estudiar las emociones y preferencias visuales de los consumidores hacia hojuelas de papas (Solanum tuberosum) nativas y clásicas. Se utilizaron dos tipos de hojuelas frita, papa clásica y papa nativa, con un costo de S/ 6,60 (1.79 USD) y S/ 7,00 (1.90 USD), respectivamente. En una primera etapa se utilizó la tecnología de Seguimiento Ocular y el Análisis de Emoción utilizando una aplicación para teléfono móvil. En una segunda etapa, cada participante respondió una encuesta cognitiva, mediante el uso de formulario Google. Se utilizaron 100 consumidores con edades entre 14 y 63 años. La encuesta mostró que un 45% de los consumidores mostraron su intención de comprar el producto nativo más que el producto clásico. Los resultados de neuromarketing mostraron principalmente una mayor proporción de neutralidad, y un poco menor, en emociones negativas de “escepticismo”, “tristeza” y “disgusto”, especialmente sobre el factor nutricional y el precio. Estos resultados, más que contradictorios son complementarios, ya que las herramientas tecnológicas nos permiten conocer más detalles de las preferencias y elecciones. Los consumidores pretenden consumir más productos nativos, siempre que sean más nutritivos, más sustentables, más baratos y beneficien al productor local.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42957252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-05DOI: 10.17268/sci.agropecu.2023.019
J. Jimenez, A. Leiva, W. Cuellar
Cassava-torrado-like virus (CsTLV) is a bipartite single-stranded RNA virus belonging to the family Secoviridae. The virus has been reported in Brazil and Colombia, and is usually found in mixed infections, and in plants affected by Cassava Frogskin Disease (CFSD), an endemic cassava disease in the Americas. Genome analysis of CsTLV identifies a gene in RNA2 implicated in pathogen movement in other torradoviruses. This gene (RNA2-ORF1) and another one no related to virus movement (Maf/Ham1) were amplified by PCR and cloned into constructs under the 35S promoter of cauliflower mosaic virus (CaMV), which then were transfered to agrobacterium binary vectors. When agro-infiltrated in Nicotiana benthamiana plants, only RNA2-ORF1 had a positive effect on the mechanical inoculation of cassava virus X (CsVX), a potexvirus that has a low rate of mechanical infection in N. benthamiana. Efficiency of CsVX mechanical transmission was measured by the number of infected plants, presence of symptoms, and titers of CsVX as measured by ELISA, two weeks after infection. On average, CsVX could infect 2.3 times more plants when these were previously agro-infiltrated with A. tumefaciens binary vector encoding CsTLV RNA2-ORF1. We conclude that the novel secovirid CsTLV associated with leaf spot symptoms in cassava, encodes a gene that could enhance other viral infections in N. benthamiana. Further studies are required to elucidate this effect and its role in mixed infections, often observed in cassava plants affected by CFSD.
类木薯病毒（Cassava torrado like virus，CsTLV）是一种二分单链RNA病毒，属于次级病毒科。该病毒已在巴西和哥伦比亚报告，通常在混合感染中发现，并在受美洲流行木薯病木薯蛙皮病（CFSD）影响的植物中发现。CsTLV的基因组分析确定了RNA2中的一个基因，该基因与其他托拉病毒的病原体运动有关。用聚合酶链式反应扩增该基因（RNA2-ORF1）和另一个与病毒运动无关的基因（Maf/Ham1），并将其克隆到花椰菜花叶病毒（CaMV）35S启动子下的构建体中，然后将其转移到农杆菌二元载体上。当农业渗透到本氏烟草植物中时，只有RNA2-ORF1对机械接种木薯病毒X（CsVX）有积极作用，该病毒是一种在本氏烟草中机械感染率较低的潜在病毒。CsVX机械传播的效率通过感染植物的数量、症状的存在以及感染后两周通过ELISA测量的CsVX滴度来测量。平均而言，当以前用编码CsTLV RNA2-ORF1的根癌分枝杆菌二元载体对这些植物进行农业渗透时，CsVX可以感染2.3倍以上的植物。我们的结论是，与木薯叶斑症状相关的新型secovirid CsTLV编码一个基因，该基因可以增强本氏猪笼草的其他病毒感染。需要进一步的研究来阐明这种影响及其在混合感染中的作用，这种感染通常在受CFSD影响的木薯植物中观察到。
{"title":"Cassava torrado-like virus encodes a gene that facilitates the mechanical transmission to Nicotiana benthamiana of Cassava virus X","authors":"J. Jimenez, A. Leiva, W. Cuellar","doi":"10.17268/sci.agropecu.2023.019","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.019","url":null,"abstract":"Cassava-torrado-like virus (CsTLV) is a bipartite single-stranded RNA virus belonging to the family Secoviridae. The virus has been reported in Brazil and Colombia, and is usually found in mixed infections, and in plants affected by Cassava Frogskin Disease (CFSD), an endemic cassava disease in the Americas. Genome analysis of CsTLV identifies a gene in RNA2 implicated in pathogen movement in other torradoviruses. This gene (RNA2-ORF1) and another one no related to virus movement (Maf/Ham1) were amplified by PCR and cloned into constructs under the 35S promoter of cauliflower mosaic virus (CaMV), which then were transfered to agrobacterium binary vectors. When agro-infiltrated in Nicotiana benthamiana plants, only RNA2-ORF1 had a positive effect on the mechanical inoculation of cassava virus X (CsVX), a potexvirus that has a low rate of mechanical infection in N. benthamiana. Efficiency of CsVX mechanical transmission was measured by the number of infected plants, presence of symptoms, and titers of CsVX as measured by ELISA, two weeks after infection. On average, CsVX could infect 2.3 times more plants when these were previously agro-infiltrated with A. tumefaciens binary vector encoding CsTLV RNA2-ORF1. We conclude that the novel secovirid CsTLV associated with leaf spot symptoms in cassava, encodes a gene that could enhance other viral infections in N. benthamiana. Further studies are required to elucidate this effect and its role in mixed infections, often observed in cassava plants affected by CFSD.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45337955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.17268/sci.agropecu.2023.015
Eduardo L. Pacheco-Sánchez, Lenin A. Guamani-Quimis, Cinara Ewerling da Rosa, Diego Portalanza, Alejandro E. Mieles, F. R. Garcés-Fiallos
Oil palm (Elaeis guineensis L.) is a crucial crop in Ecuador, considerably affected by black palm weevil Rhynchophorus palmarum L. (Coleoptera: Curculionidae) for several years. Despite its importance, the behavior of the black weevil in Ecuador is not well comprehended presently. Therefore, this study aimed to predict infestation patterns of the black palm weevil in Ecuador using a mathematical model based on monitoring data. Data on the number of insects per trap from a commercial oil palm farm in Quinindé, Ecuador, was collected every two weeks for five years (2016-2020) and analyzed using the Classical Fourier (CF) spectrum and the Dickey-Fuller test to determine seasonality. The trend component of the data dropped from 16.33 in January 2017 to 11.96 in January 2019, with a fluctuation ranging from -0.11 to 2.50 observed for the entire data set. The results obtained after fitting the model ranged from -0.11 to 3.19, with a maximum of 5.30. The augmented Dickey-Fuller (ADF) test for the black weevil time series yielded a result of -5.60 (P<0.01). The partial autocorrelation ranged from -0.35 to 0.1. Based on our model, we projected the occurrence of black palm weevil from 2021 to 2024, with a fluctuation in the number of insects per trap ranging from 12.68 in January 2021 to 13.023 in November 2023. This model can be used to predict future insect occurrences in Ecuador, providing valuable insights into the behavior of the black weevil and using it for effective development control measures for this pest.
{"title":"Forecasting occurrence of palm weevil Rhynchophorus palmarum L. (Coleoptera, Curculionidae) using Autoregressive Integrated Moving Average modeling","authors":"Eduardo L. Pacheco-Sánchez, Lenin A. Guamani-Quimis, Cinara Ewerling da Rosa, Diego Portalanza, Alejandro E. Mieles, F. R. Garcés-Fiallos","doi":"10.17268/sci.agropecu.2023.015","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.015","url":null,"abstract":"Oil palm (Elaeis guineensis L.) is a crucial crop in Ecuador, considerably affected by black palm weevil Rhynchophorus palmarum L. (Coleoptera: Curculionidae) for several years. Despite its importance, the behavior of the black weevil in Ecuador is not well comprehended presently. Therefore, this study aimed to predict infestation patterns of the black palm weevil in Ecuador using a mathematical model based on monitoring data. Data on the number of insects per trap from a commercial oil palm farm in Quinindé, Ecuador, was collected every two weeks for five years (2016-2020) and analyzed using the Classical Fourier (CF) spectrum and the Dickey-Fuller test to determine seasonality. The trend component of the data dropped from 16.33 in January 2017 to 11.96 in January 2019, with a fluctuation ranging from -0.11 to 2.50 observed for the entire data set. The results obtained after fitting the model ranged from -0.11 to 3.19, with a maximum of 5.30. The augmented Dickey-Fuller (ADF) test for the black weevil time series yielded a result of -5.60 (P<0.01). The partial autocorrelation ranged from -0.35 to 0.1. Based on our model, we projected the occurrence of black palm weevil from 2021 to 2024, with a fluctuation in the number of insects per trap ranging from 12.68 in January 2021 to 13.023 in November 2023. This model can be used to predict future insect occurrences in Ecuador, providing valuable insights into the behavior of the black weevil and using it for effective development control measures for this pest.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47448908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.17268/sci.agropecu.2023.018
Yuly Kety Espinoza-Leandro, Luis Olivera-Montenegro, Perla Paredes-Concepción
Applications of formulations that incorporate emulsified oils offer interesting prospects for producing foods with better characteristics that are in line with the new trends in healthy eating and the new regulations on nutritional claims. This review defines and classifies the types of emulsions according to their drop size, formulation and analyzes the latest research related to the application of emulsions in the food industry. It presents valuable information on the use of micro, nano and macro emulsions for the preparation of meat, dairy and vegetable products as a potential alternative in the transport of bioactive compounds, reformulations with better lipid profiles and substitution ingredients of meat origin by ingredients of plant origin, obtaining foods with acceptable functional and sensory properties. New advances in the emulsion industry open up great possibilities in the delivery of health products, however, there are still limitations in the use of ingredients of plant origin in products formulated in considerable percentages with ingredients of animal origin. There are opportunities to improve the technology of food emulsion, the application at an industrial level is a step that is being taken little by little in congruence with the economic viability of the use of vegetable oils in the reformulation and the demand of current consumers for delivery of products with a clean label. It is suggested to study the economic feasibility of the industrial application of the use of single and double emulsion systems as a substitute ingredient for animal fats.
{"title":"Meat, dairy and vegetable emulsions: Recent innovations in the development of functional, healthy and more stable foods","authors":"Yuly Kety Espinoza-Leandro, Luis Olivera-Montenegro, Perla Paredes-Concepción","doi":"10.17268/sci.agropecu.2023.018","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.018","url":null,"abstract":"Applications of formulations that incorporate emulsified oils offer interesting prospects for producing foods with better characteristics that are in line with the new trends in healthy eating and the new regulations on nutritional claims. This review defines and classifies the types of emulsions according to their drop size, formulation and analyzes the latest research related to the application of emulsions in the food industry. It presents valuable information on the use of micro, nano and macro emulsions for the preparation of meat, dairy and vegetable products as a potential alternative in the transport of bioactive compounds, reformulations with better lipid profiles and substitution ingredients of meat origin by ingredients of plant origin, obtaining foods with acceptable functional and sensory properties. New advances in the emulsion industry open up great possibilities in the delivery of health products, however, there are still limitations in the use of ingredients of plant origin in products formulated in considerable percentages with ingredients of animal origin. There are opportunities to improve the technology of food emulsion, the application at an industrial level is a step that is being taken little by little in congruence with the economic viability of the use of vegetable oils in the reformulation and the demand of current consumers for delivery of products with a clean label. It is suggested to study the economic feasibility of the industrial application of the use of single and double emulsion systems as a substitute ingredient for animal fats.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46754234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.17268/sci.agropecu.2023.017
E. Contreras-López, Eida Quispe-Mitma, A. Muñoz, R. Yuli-Posadas, Marcelo Portuguez-Maurtua, Félix Hugo Milla Flores
There are several investigations on the use of food waste to remove contaminants by adsorption. However, a simple route, without chemical activation reagents, is needed for the development of adsorbents. The aim of this study was to develop an adsorbent from plantain peel, using a simple procedure, and to evaluate its capacity to remove phosphate from aqueous solutions at phosphate concentrations similar to those of water sources at risk of eutrophication (0.30 mg/L). The simple pyrolysis method was used in an electric muffle, without chemical activation, using plantain peel as precursor. The variables evaluated were pyrolysis temperature and solution pH. The specific surface area BET, zero loading point of the developed treatments, was determined. Phosphate adsorption was studied in a batch experiment in the presence of calcium ions in solution. Phosphate adsorption was favorable at all three pyrolysis temperature levels (500, 600 and 700 °C) and two solution pH levels (pH 7 and 10). the pseudo-second order kinetic model was the best fit for the experimental data to describe the adsorption mechanism. The best fit to the experimental equilibrium data was obtained with the Langmuir isotherm model. It was found that a 1 g/L dose of the adsorbent was able to reduce 92% of phosphate in water, with a removal capacity 0.14 mg/g at pH 10 and pyrolysis temperature of 700 °C. This study lays the groundwork for future research on the use of this type of adsorbent in water treatment to facilitate access to clean water for rural populations.
{"title":"Plantain peel adsorbent: Simple preparation, and adsorption at phosphate concentrations similar to those of water sources at risk of eutrophication","authors":"E. Contreras-López, Eida Quispe-Mitma, A. Muñoz, R. Yuli-Posadas, Marcelo Portuguez-Maurtua, Félix Hugo Milla Flores","doi":"10.17268/sci.agropecu.2023.017","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.017","url":null,"abstract":"There are several investigations on the use of food waste to remove contaminants by adsorption. However, a simple route, without chemical activation reagents, is needed for the development of adsorbents. The aim of this study was to develop an adsorbent from plantain peel, using a simple procedure, and to evaluate its capacity to remove phosphate from aqueous solutions at phosphate concentrations similar to those of water sources at risk of eutrophication (0.30 mg/L). The simple pyrolysis method was used in an electric muffle, without chemical activation, using plantain peel as precursor. The variables evaluated were pyrolysis temperature and solution pH. The specific surface area BET, zero loading point of the developed treatments, was determined. Phosphate adsorption was studied in a batch experiment in the presence of calcium ions in solution. Phosphate adsorption was favorable at all three pyrolysis temperature levels (500, 600 and 700 °C) and two solution pH levels (pH 7 and 10). the pseudo-second order kinetic model was the best fit for the experimental data to describe the adsorption mechanism. The best fit to the experimental equilibrium data was obtained with the Langmuir isotherm model. It was found that a 1 g/L dose of the adsorbent was able to reduce 92% of phosphate in water, with a removal capacity 0.14 mg/g at pH 10 and pyrolysis temperature of 700 °C. This study lays the groundwork for future research on the use of this type of adsorbent in water treatment to facilitate access to clean water for rural populations.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43089566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.17268/sci.agropecu.2023.016
C. O. Arévalo-Hernández, E. Arévalo, Juvicksa Correa, Mahiler Mirco Vasquez Gonzales, Dapeng Zhang
Peru has a great potential for forestry plantations, nevertheless, importations of this type of products are getting very expensive. Soils under forest are acidic and infertile to support sustainable economically valuable forest tree species such as teak. Information is lacking on proper fertility management in forest plantations such as teak. Therefore, the objective of this study was to explore the effect of lime, and organic and inorganic fertilizers on the early growth and nutrient composition of 12-month old teak grown on acidic soil of Peru. The soil under field study was acidic with a pH of 4.99, low in fertility. A factorial design of 2x3: Lime (No Lime and Liming) and fertilizer (organic, inorganic and mixture), with 9 repetitions was adopted. Tree biometric parameters (height, diameter, biomass) and N, P, K, Ca, Mg, Fe, Cu, Mn, Zn foliar concentrations were determined during early the growth of teak. Treatments with addition of dolomite lime favored higher biometric parameters, and use of organic fertilizers promoted more growth than inorganic fertilizers. In the case of nutrients, no major differences were observed between limed and unlimed treatments while organic fertilizer promoted Ca, K and S nutrition. The results show that the application of lime and organic fertilizers is essential for the successful management and establishment of teak plants in acidic soils of Peru.
{"title":"Effect of liming and fertilizers on the growth and nutrition of 12-month old Teak (Tectona grandis L.) grown on acidic soil of Peru","authors":"C. O. Arévalo-Hernández, E. Arévalo, Juvicksa Correa, Mahiler Mirco Vasquez Gonzales, Dapeng Zhang","doi":"10.17268/sci.agropecu.2023.016","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.016","url":null,"abstract":"Peru has a great potential for forestry plantations, nevertheless, importations of this type of products are getting very expensive. Soils under forest are acidic and infertile to support sustainable economically valuable forest tree species such as teak. Information is lacking on proper fertility management in forest plantations such as teak. Therefore, the objective of this study was to explore the effect of lime, and organic and inorganic fertilizers on the early growth and nutrient composition of 12-month old teak grown on acidic soil of Peru. The soil under field study was acidic with a pH of 4.99, low in fertility. A factorial design of 2x3: Lime (No Lime and Liming) and fertilizer (organic, inorganic and mixture), with 9 repetitions was adopted. Tree biometric parameters (height, diameter, biomass) and N, P, K, Ca, Mg, Fe, Cu, Mn, Zn foliar concentrations were determined during early the growth of teak. Treatments with addition of dolomite lime favored higher biometric parameters, and use of organic fertilizers promoted more growth than inorganic fertilizers. In the case of nutrients, no major differences were observed between limed and unlimed treatments while organic fertilizer promoted Ca, K and S nutrition. The results show that the application of lime and organic fertilizers is essential for the successful management and establishment of teak plants in acidic soils of Peru.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49248563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.17268/sci.agropecu.2023.012
G. E. Delgado-Paredes, C. Rojas-Idrogo, Cecilia Vásquez-Díaz
Cucurbitaceae species constitute a family with a wide worldwide distribution and great nutritional and medicinal importance. The Cucurbita genus is the most important of the family with valuable species such as C. maxima and C. moschata. However, the large-scale production of sex specific plants using the conventional propagation methods has various limitations. The tissue culture such as micropropagation can help overcome these limitations. The aim of this study was to establish a protocol for ex vitro and in vitro clonal propagation of two varieties of Cucurbita moschata: ‘loche’, a variety of commercial vegetative propagation and endemic in Lambayeque, and ‘chuyán’ another variety of very restricted cultivation in some Andean regions of the north of the Peru. Likewise, the in vitro propagation of ‘chisguín’ (C. ecuadorensis), a threatened endemic wild species from the coast of Ecuador. ‘Loche’ was propagated ex vitro by microcutting with a node and leaf, and micropropagated in MS culture medium supplemented with 0.02 mg L-1 IAA and 0.02 mg L-1 GA3. Seedlings of ‘chuyán’ and ‘chisguin’ were micropropagated in MS with with 0.02 mg L-1 IAA, 0.5 mg L-1 BAP and 0.02 mg L-1 GA3. This study constitutes the first report on micropropagation of Peruvian and Ecuadorian varieties and species of the Cucurbita genus.
{"title":"Protocol for ex vitro and in vitro micropropagation of Cucurbita moschata and C. ecuadorensis, native to Peru and Ecuador, of nutritional and medicinal importance","authors":"G. E. Delgado-Paredes, C. Rojas-Idrogo, Cecilia Vásquez-Díaz","doi":"10.17268/sci.agropecu.2023.012","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.012","url":null,"abstract":"Cucurbitaceae species constitute a family with a wide worldwide distribution and great nutritional and medicinal importance. The Cucurbita genus is the most important of the family with valuable species such as C. maxima and C. moschata. However, the large-scale production of sex specific plants using the conventional propagation methods has various limitations. The tissue culture such as micropropagation can help overcome these limitations. The aim of this study was to establish a protocol for ex vitro and in vitro clonal propagation of two varieties of Cucurbita moschata: ‘loche’, a variety of commercial vegetative propagation and endemic in Lambayeque, and ‘chuyán’ another variety of very restricted cultivation in some Andean regions of the north of the Peru. Likewise, the in vitro propagation of ‘chisguín’ (C. ecuadorensis), a threatened endemic wild species from the coast of Ecuador. ‘Loche’ was propagated ex vitro by microcutting with a node and leaf, and micropropagated in MS culture medium supplemented with 0.02 mg L-1 IAA and 0.02 mg L-1 GA3. Seedlings of ‘chuyán’ and ‘chisguin’ were micropropagated in MS with with 0.02 mg L-1 IAA, 0.5 mg L-1 BAP and 0.02 mg L-1 GA3. This study constitutes the first report on micropropagation of Peruvian and Ecuadorian varieties and species of the Cucurbita genus.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42900470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.17268/sci.agropecu.2023.009
M. V. Rodríguez-Deméneghi, N. Aguilar-Rivera, Y. A. Gheno-Heredia, A. A. Armas-Silva
Vanilla is the orchid of greatest economic interest to the world since the spice of the same name is obtained from it. Vanillin is obtained from its ripened pod, this compound is highly recognized and demanded by the gastronomic, pharmaceutical, and cosmetic industries for the sweet aroma and flavor it emanates. The objective of this work was to carry out a literature review to put into perspective the reader about the sustainability strategies in the vanilla industry in Mexico, as well as the innovations of propagation and in vitro conservation of Vanilla planifolia germplasm. Despite being originally from Mexico, this country ranks third in world production, preceding Madagascar, and Indonesia. This compilation of studies presents biotechnological techniques to increase vanilla production, as well as alternatives that can reduce the negative effects caused by the low genetic accumulation in this species, such as plant tissue culture, use of molecular markers for the study of somaclonal variation, asymbiotic seed germination under in vitro conditions. These techniques together with agro-industrial strategies can represent a sustainable alternative for vanilla production. In this article, strategies are suggested that allow those interested in vanilla production to make appropriate decisions, however, it is necessary to develop multidisciplinary studies that allow a global approach to the phenomena involved in the production and use of vanilla, as well as the resolution of the problems that this process faces.
{"title":"Vanilla cultivation in Mexico: Typology, characteristics, production, agroindustrial prospective and biotechnological innovations as a sustainability strategy","authors":"M. V. Rodríguez-Deméneghi, N. Aguilar-Rivera, Y. A. Gheno-Heredia, A. A. Armas-Silva","doi":"10.17268/sci.agropecu.2023.009","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.009","url":null,"abstract":"Vanilla is the orchid of greatest economic interest to the world since the spice of the same name is obtained from it. Vanillin is obtained from its ripened pod, this compound is highly recognized and demanded by the gastronomic, pharmaceutical, and cosmetic industries for the sweet aroma and flavor it emanates. The objective of this work was to carry out a literature review to put into perspective the reader about the sustainability strategies in the vanilla industry in Mexico, as well as the innovations of propagation and in vitro conservation of Vanilla planifolia germplasm. Despite being originally from Mexico, this country ranks third in world production, preceding Madagascar, and Indonesia. This compilation of studies presents biotechnological techniques to increase vanilla production, as well as alternatives that can reduce the negative effects caused by the low genetic accumulation in this species, such as plant tissue culture, use of molecular markers for the study of somaclonal variation, asymbiotic seed germination under in vitro conditions. These techniques together with agro-industrial strategies can represent a sustainable alternative for vanilla production. In this article, strategies are suggested that allow those interested in vanilla production to make appropriate decisions, however, it is necessary to develop multidisciplinary studies that allow a global approach to the phenomena involved in the production and use of vanilla, as well as the resolution of the problems that this process faces.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44214930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.17268/sci.agropecu.2023.006
Michael Niño-de-Guzman-Tito, I. Lima-Medina
The attributes of nematodes are presented as valuable tools for determining the quality of soil, especially that of mining companies. The aim of this study was to evaluate the behavior of nematodes against a stress gradient in a rainy season and a dry season in soils influenced by mining. Thus, field sampling was carried out over 100 m2 in triplicate for four types of soils classified according to their uses (pasture, maize cultivation, fig cultivation, and eucalyptus cultivation), and these areas were located on the periphery (500 to 1500 m) of the Ollachea mining community of Puno in Peru; subsequently, the samples were processed in the laboratory to determine edaphic, agrochemical, heavy metal, and microbiological parameters and identify the nematodes. The abiotic stress gradient was determined by a principal component analysis; and through a canonical correlation analysis, the relationships between the abiotic stress gradient and the nematodes were determined. Canonical correlation analysis showed that there were significant correlations: in the rainy season, Helicotylenchus and vanadium (r = 0.99), Globodera and titanium (r = 0.97), and Tylenchus and lead (r = 0.96); in the dry season: Meloidogyne and vanadium (r = 0.99), and Hemicycliophora and lead (r = 0.91). In conclusion, the abiotic stress gradient had a high correlation with bacterivorous, fungivorous, and phytoparasitic nematodes and a low correlation with omnivorous and predatory nematodes, showing the bioindicator capacity of nematodes in relation to stress parameters that impact soil quality.
{"title":"Bioindicator nematodes in relation to an abiotic stress gradient in soils influenced by mining","authors":"Michael Niño-de-Guzman-Tito, I. Lima-Medina","doi":"10.17268/sci.agropecu.2023.006","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.006","url":null,"abstract":"The attributes of nematodes are presented as valuable tools for determining the quality of soil, especially that of mining companies. The aim of this study was to evaluate the behavior of nematodes against a stress gradient in a rainy season and a dry season in soils influenced by mining. Thus, field sampling was carried out over 100 m2 in triplicate for four types of soils classified according to their uses (pasture, maize cultivation, fig cultivation, and eucalyptus cultivation), and these areas were located on the periphery (500 to 1500 m) of the Ollachea mining community of Puno in Peru; subsequently, the samples were processed in the laboratory to determine edaphic, agrochemical, heavy metal, and microbiological parameters and identify the nematodes. The abiotic stress gradient was determined by a principal component analysis; and through a canonical correlation analysis, the relationships between the abiotic stress gradient and the nematodes were determined. Canonical correlation analysis showed that there were significant correlations: in the rainy season, Helicotylenchus and vanadium (r = 0.99), Globodera and titanium (r = 0.97), and Tylenchus and lead (r = 0.96); in the dry season: Meloidogyne and vanadium (r = 0.99), and Hemicycliophora and lead (r = 0.91). In conclusion, the abiotic stress gradient had a high correlation with bacterivorous, fungivorous, and phytoparasitic nematodes and a low correlation with omnivorous and predatory nematodes, showing the bioindicator capacity of nematodes in relation to stress parameters that impact soil quality.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44572247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-22DOI: 10.17268/sci.agropecu.2023.008
W. E. Núñez, D. Sotomayor, C. V. Ballardo, E. Herrera
The compost produced based on municipal/urban organic solid waste (RSOM/U), is a valuable resource as a biofertilizer for agriculture, gardening, forestry and especially for soil remediation, whose production contributes to sustainable development through recycling of organic matter and nutrients. However, due to the raw materials used, the compost can have a significant content of heavy metals such as: cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg) and selenium (Se), and contaminants such as potentially toxic trace elements that harm human health. These components can accumulate in plant tissues by absorption, giving rise to the possibility of being bioavailable to humans and animals. The fungal bioremediation of heavy metals in RSOM/U compost is highly efficient, cost-effective, available and friendly to the environment, therefore the removal of metals through this technique is a priority, if the purpose is to use it in agricultural soils. This review summarizes the studies based on the potential of fungal biomass for the bioremediation of heavy metals in RSOM/U compost, reporting information on RSOM/U-based compost, fungal biomass production and mechanisms of bioremediation of heavy metals by fungal biomass. In conclusion, the bioremediation of heavy metals using the fungal biomass in RSOM/U compost, with adequate segregation of raw material, coupled with bioremediation, could improve the removal of heavy metals in RSOM/U compost, and could be an ecological and viable alternative, which must be valued by intensifying its use.
{"title":"Fungal biomass potential: production and bioremediation mechanisms of heavy metals from municipal organic solid waste compost","authors":"W. E. Núñez, D. Sotomayor, C. V. Ballardo, E. Herrera","doi":"10.17268/sci.agropecu.2023.008","DOIUrl":"https://doi.org/10.17268/sci.agropecu.2023.008","url":null,"abstract":"The compost produced based on municipal/urban organic solid waste (RSOM/U), is a valuable resource as a biofertilizer for agriculture, gardening, forestry and especially for soil remediation, whose production contributes to sustainable development through recycling of organic matter and nutrients. However, due to the raw materials used, the compost can have a significant content of heavy metals such as: cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg) and selenium (Se), and contaminants such as potentially toxic trace elements that harm human health. These components can accumulate in plant tissues by absorption, giving rise to the possibility of being bioavailable to humans and animals. The fungal bioremediation of heavy metals in RSOM/U compost is highly efficient, cost-effective, available and friendly to the environment, therefore the removal of metals through this technique is a priority, if the purpose is to use it in agricultural soils. This review summarizes the studies based on the potential of fungal biomass for the bioremediation of heavy metals in RSOM/U compost, reporting information on RSOM/U-based compost, fungal biomass production and mechanisms of bioremediation of heavy metals by fungal biomass. In conclusion, the bioremediation of heavy metals using the fungal biomass in RSOM/U compost, with adequate segregation of raw material, coupled with bioremediation, could improve the removal of heavy metals in RSOM/U compost, and could be an ecological and viable alternative, which must be valued by intensifying its use.","PeriodicalId":21642,"journal":{"name":"Scientia Agropecuaria","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43468104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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