Pub Date : 2021-01-01DOI: 10.15587/2519-4798.2021.240151
V. Olkhovska, Z. Yeloyeva, Yevhen Olkhovskyi
The aim of the work was to study the characteristics of the response of non-specific protective factors in tonsillitis of streptococcal etiology in children infected and uninfected with HHV-6 infection at different periods of the disease. Materials and research methods. In 78 children aged 3–15 years, inflammation markers and phagocytic activity of peripheral blood neutrophils (PAN) were determined using the nitroblue tetrazolium reduction test. The studies were carried out in the acute period and in the period of convalescence. Mathematical and statistical processing of the data obtained was carried out using Microsoft Excel 2003 and Statistica 6.0 programs. The significance of differences between the mean values was determined using the Student's test (t). Results. It was found that infection of children with HHV-6 leads to a decrease in the functional activity of neutrophils in the onset of streptococcal tonsillitis compared with uninfected patients (p<0.05). Also, the acute period of streptococcal tonsillitis in patients with co-infection is accompanied by a violation of the functional reserve of the oxygen-dependent mechanism of bactericidal neutrophils: minimal opportunities were found in tonsillitis on the background of HHV-6 infection (p<0.05). By the period of convalescence, a decrease in the indicators of the functional activity of neutrophils, increased in the acute period, was recorded, but in children infected with HHV-6, a complete recovery of indicators to the physiological norm did not occur. One of the features of streptococcal tonsillitis in children with HHV-6 infection is an increase in serum gamma globulin level (p˂0.05), lack of significant dynamics of decrease in the ASL-O indicator. Conclusions. The presence of VHL-6t infection in a child with streptococcal tonsillitis adversely affects the state of nonspecific protective factors, in particular PAN. The obtained data must be used to optimize the management of patients at different periods of the disease, including at the stages of dispensary observation
{"title":"Non-specific factors of children protection with streptococcal tonsillitis and infected with herpes virus type 6","authors":"V. Olkhovska, Z. Yeloyeva, Yevhen Olkhovskyi","doi":"10.15587/2519-4798.2021.240151","DOIUrl":"https://doi.org/10.15587/2519-4798.2021.240151","url":null,"abstract":"The aim of the work was to study the characteristics of the response of non-specific protective factors in tonsillitis of streptococcal etiology in children infected and uninfected with HHV-6 infection at different periods of the disease. Materials and research methods. In 78 children aged 3–15 years, inflammation markers and phagocytic activity of peripheral blood neutrophils (PAN) were determined using the nitroblue tetrazolium reduction test. The studies were carried out in the acute period and in the period of convalescence. Mathematical and statistical processing of the data obtained was carried out using Microsoft Excel 2003 and Statistica 6.0 programs. The significance of differences between the mean values was determined using the Student's test (t). Results. It was found that infection of children with HHV-6 leads to a decrease in the functional activity of neutrophils in the onset of streptococcal tonsillitis compared with uninfected patients (p<0.05). Also, the acute period of streptococcal tonsillitis in patients with co-infection is accompanied by a violation of the functional reserve of the oxygen-dependent mechanism of bactericidal neutrophils: minimal opportunities were found in tonsillitis on the background of HHV-6 infection (p<0.05). By the period of convalescence, a decrease in the indicators of the functional activity of neutrophils, increased in the acute period, was recorded, but in children infected with HHV-6, a complete recovery of indicators to the physiological norm did not occur. One of the features of streptococcal tonsillitis in children with HHV-6 infection is an increase in serum gamma globulin level (p˂0.05), lack of significant dynamics of decrease in the ASL-O indicator. Conclusions. The presence of VHL-6t infection in a child with streptococcal tonsillitis adversely affects the state of nonspecific protective factors, in particular PAN. The obtained data must be used to optimize the management of patients at different periods of the disease, including at the stages of dispensary observation","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78014197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-28DOI: 10.15587/2519-4798.2020.221120
E. Murzina
The aim. To study the effect of increased body weight in children with psoriasis on the indicators of the biochemical profile and the severity of the pathological process. Materials and methods. The indicators of the biochemical profile were studied in 108 children, namely: the level of total protein, total bilirubin, cholesterol, liver enzymes (ALT, AST, GGT), triglycerides and low density lipoproteins, creatinine, urea, uric acid (UA), depending on the mass index body (BMI). The research materials were statistically processed using parametric analysis methods using the STATISTICA 13.3 software (developed by StatSoft. Inc). Results. Lipid profile indices both in the group of children with normal BMI and in the group of children with increased BMI are within the reference values. Statistically significant differences were found between the average GGT level in the group of children with increased BMI compared with the indicators in the group of children with normal BMI and indicators in the children of the control group. Also, in children with increased BMI, the average UA level is statistically significant higher than in children of the control group and has direct moderate and significant correlations with indices of the severity of the pathological process. In children with increased BMI, psoriasis severity indices correlate with BMI: PGA (r=0.51, p<0,01) BSA (r=0.48, p<0,01), PASI at the beginning of treatment (r=0.41, p<0,05) and PASI at the end of treatment (r=0.67, p<0.001) and percentage of excess body weight: BSA (r=0.34, p<0,05), PASI at the end of treatment (r=0.67, p<0.001). In children with normal BMI, such correlations were not found
{"title":"Biochemical indicators and course of dermatosis in children with psoriasis depending on the body mass index","authors":"E. Murzina","doi":"10.15587/2519-4798.2020.221120","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.221120","url":null,"abstract":"The aim. To study the effect of increased body weight in children with psoriasis on the indicators of the biochemical profile and the severity of the pathological process. Materials and methods. The indicators of the biochemical profile were studied in 108 children, namely: the level of total protein, total bilirubin, cholesterol, liver enzymes (ALT, AST, GGT), triglycerides and low density lipoproteins, creatinine, urea, uric acid (UA), depending on the mass index body (BMI). The research materials were statistically processed using parametric analysis methods using the STATISTICA 13.3 software (developed by StatSoft. Inc). Results. Lipid profile indices both in the group of children with normal BMI and in the group of children with increased BMI are within the reference values. Statistically significant differences were found between the average GGT level in the group of children with increased BMI compared with the indicators in the group of children with normal BMI and indicators in the children of the control group. Also, in children with increased BMI, the average UA level is statistically significant higher than in children of the control group and has direct moderate and significant correlations with indices of the severity of the pathological process. In children with increased BMI, psoriasis severity indices correlate with BMI: PGA (r=0.51, p<0,01) BSA (r=0.48, p<0,01), PASI at the beginning of treatment (r=0.41, p<0,05) and PASI at the end of treatment (r=0.67, p<0.001) and percentage of excess body weight: BSA (r=0.34, p<0,05), PASI at the end of treatment (r=0.67, p<0.001). In children with normal BMI, such correlations were not found","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86715828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-28DOI: 10.15587/2519-4798.2020.221130
M. Sloboda, T. Pupin
The development and course of periodontal disease in young people is characterized by sluggish clinical symptoms, and at the initial stage are asymptomatic, which greatly complicates its timely diagnosis. Therefore, it is especially important to find methods of early diagnosis and prediction of periodontal disease, which will allow for a rational comprehensive prevention. Among the causes of periodontal disease, the main role belongs to the microorganisms of dental plaque, which has important diagnostic value. The aim of the study. To investigate the composition of mixed microcultures of subgingival plaque bacteria in young people with different nosological forms of periodontal disease and to assess the degree of influence of microbial factors on the development of periodontal diseases. Materials and methods. The state of microbiocenosis in the subgingival zone in 104 people with periodontal disease and in 94 people with intact periodontal tissues were studied. To assess the severity of the influence of the types of selected microcultures of the subgingival zone on the development and progression of periodontal disease in young people, the method of alternative sequential analysis of Wald and Bayes’s formula was used. Results. With the development of the inflammatory process in periodontal tissues in most cases revealed cultures characterized by the presence of polymorphic gram-negative bacteria that developed, fixed on the solid phase, and in the liquid phase contained coccal microflora (53.01±5.48 %). During the development of generalized periodontitis, an increasing number of microcultures with a predominance of gram-negative microflora with pronounced adhesive properties was detected, which led to the development of these microcultures on the solid phase (61,9±10,6 %). The calculation of prognostic coefficients showed that the presence of microcultures that developed in the planktonic phase, but contained gram-negative rod-shaped bacteria and cocci were highly important in terms of the development of periodontal disease (+7.46). Conclusions. With the development of the pathological process in periodontal tissues, the state of the microflora changed in the direction of increasing gram-negative polymorphic microflora with pronounced adhesive properties that are capable of aggregation (up to 53.01 % in patients with catarrhal gingivitis, up to 90,48 % – with generalized periodontitis). The result of calculation of the severity of isolated types of microcultures from subgingival zone on the development and progression of periodontal disease in young people showed that the presence of microcultures that developed in the planktonic phase, but contained gram-negative rod-shaped bacteria and cocci were highly important in terms of the development of periodontal disease
{"title":"The degree of influence of the types of selected microcultures from subgingival zone on diferent nosological forms of periodontal diseases in young people","authors":"M. Sloboda, T. Pupin","doi":"10.15587/2519-4798.2020.221130","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.221130","url":null,"abstract":"The development and course of periodontal disease in young people is characterized by sluggish clinical symptoms, and at the initial stage are asymptomatic, which greatly complicates its timely diagnosis. Therefore, it is especially important to find methods of early diagnosis and prediction of periodontal disease, which will allow for a rational comprehensive prevention. Among the causes of periodontal disease, the main role belongs to the microorganisms of dental plaque, which has important diagnostic value. The aim of the study. To investigate the composition of mixed microcultures of subgingival plaque bacteria in young people with different nosological forms of periodontal disease and to assess the degree of influence of microbial factors on the development of periodontal diseases. Materials and methods. The state of microbiocenosis in the subgingival zone in 104 people with periodontal disease and in 94 people with intact periodontal tissues were studied. To assess the severity of the influence of the types of selected microcultures of the subgingival zone on the development and progression of periodontal disease in young people, the method of alternative sequential analysis of Wald and Bayes’s formula was used. Results. With the development of the inflammatory process in periodontal tissues in most cases revealed cultures characterized by the presence of polymorphic gram-negative bacteria that developed, fixed on the solid phase, and in the liquid phase contained coccal microflora (53.01±5.48 %). During the development of generalized periodontitis, an increasing number of microcultures with a predominance of gram-negative microflora with pronounced adhesive properties was detected, which led to the development of these microcultures on the solid phase (61,9±10,6 %). The calculation of prognostic coefficients showed that the presence of microcultures that developed in the planktonic phase, but contained gram-negative rod-shaped bacteria and cocci were highly important in terms of the development of periodontal disease (+7.46). Conclusions. With the development of the pathological process in periodontal tissues, the state of the microflora changed in the direction of increasing gram-negative polymorphic microflora with pronounced adhesive properties that are capable of aggregation (up to 53.01 % in patients with catarrhal gingivitis, up to 90,48 % – with generalized periodontitis). The result of calculation of the severity of isolated types of microcultures from subgingival zone on the development and progression of periodontal disease in young people showed that the presence of microcultures that developed in the planktonic phase, but contained gram-negative rod-shaped bacteria and cocci were highly important in terms of the development of periodontal disease","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74085741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.15587/2519-4798.2020.220021
S. Estrin, T. Kravchenko, A. Pechenenko
The aim: to study the morphological and morphometric changes in the myocardium against the background of cellular cardiomyoplasty in experimental myocardial infarction. Materials and methods: the experiment was carried out on 142 Wistar-Kyoto rats weighing 200–220 g, which were kept in the vivarium of the Department of Experimental Surgery of the State Institution “Inst itute of Emergency and Reconstructive Surgery named after V. K. Gusak of the National Academy of Medical Sciences of Ukraine” in the period from 2012 to 2013. The Wistar-Kyoto breed was used because it is inbred, which minimizes the rejection reaction, due to its genetic homogeneity. The animals were kept in a vivarium under conditions of 12-hour daylight hours, room temperature and access to water and food at libitum at an air temperature of +20 – + 22 °C, humidity no more than 50 %, in a light mode day-night. The use of animals in the experiment was carried out in accordance with the rules regulated by the “European Convention for the Supervision and Protection of Vertebrate Animals used for Experimental and Ot her Scientific Purposes” (Strasbourg, 1986), Directives of the Council of the European Union of November 24, 1986 and the order of the Ministry of Health of Ukraine No. 32 dated 02.22.88. The induction of my ocardial infarction (MI) was carried out according to the technique developed by us under general anesth esia. A separate group consisted of 20 males, whom we used as donors of mesenchymal stem cells (MSC) for further research on the Y chromosome of cell homing in the body. Cultivation of MSCs was carried out in a mixture of nutrient media DMEM / F12, 1:1, (Sigma, USA). The material for morphological studies was the sections of the myocardium of laboratory animals. To assess the morphometric parameters, histochemical methods were performed according to the recipes, which are given in the instructions for histochemistry. Immunohistochemical study was performed on paraffin sections with a thickness of 5-6 μm by the indirect Koons method according to the Brosman method (1979). Results: it was found that cellular cardiomyoplasty significantly improves the structure of the postinfarction heart, manifests itself in a decrease in the scar area and connective tissue, respectively, in an increase in the number of vessels and the percentage of preserved muscle fibers. The best results were achieved with intramyocardial injection, which requires confirmation of this fact in a clinical study. Conclusions: cellular cardiomyoplasty with any method of introducing a cell graft has a positive effect both on the morphological substrate of the heart in the form of a decrease in the size of the scar during postinfarction remodeling, an increase in the number of newly formed vessels and an increase in the percentage of preserved cardiomyocytes. This occurs due to the homing of MSCs into the ischemic zone and the commonality of two mechanisms – direct differentiation into endothelial cells
{"title":"Morphological and morphometric changes on the background of cell cardiomyoplasty in experimental myocardial infarction","authors":"S. Estrin, T. Kravchenko, A. Pechenenko","doi":"10.15587/2519-4798.2020.220021","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.220021","url":null,"abstract":"The aim: to study the morphological and morphometric changes in the myocardium against the background of cellular cardiomyoplasty in experimental myocardial infarction. Materials and methods: the experiment was carried out on 142 Wistar-Kyoto rats weighing 200–220 g, which were kept in the vivarium of the Department of Experimental Surgery of the State Institution “Inst itute of Emergency and Reconstructive Surgery named after V. K. Gusak of the National Academy of Medical Sciences of Ukraine” in the period from 2012 to 2013. The Wistar-Kyoto breed was used because it is inbred, which minimizes the rejection reaction, due to its genetic homogeneity. The animals were kept in a vivarium under conditions of 12-hour daylight hours, room temperature and access to water and food at libitum at an air temperature of +20 – + 22 °C, humidity no more than 50 %, in a light mode day-night. The use of animals in the experiment was carried out in accordance with the rules regulated by the “European Convention for the Supervision and Protection of Vertebrate Animals used for Experimental and Ot her Scientific Purposes” (Strasbourg, 1986), Directives of the Council of the European Union of November 24, 1986 and the order of the Ministry of Health of Ukraine No. 32 dated 02.22.88. The induction of my ocardial infarction (MI) was carried out according to the technique developed by us under general anesth esia. A separate group consisted of 20 males, whom we used as donors of mesenchymal stem cells (MSC) for further research on the Y chromosome of cell homing in the body. Cultivation of MSCs was carried out in a mixture of nutrient media DMEM / F12, 1:1, (Sigma, USA). The material for morphological studies was the sections of the myocardium of laboratory animals. To assess the morphometric parameters, histochemical methods were performed according to the recipes, which are given in the instructions for histochemistry. Immunohistochemical study was performed on paraffin sections with a thickness of 5-6 μm by the indirect Koons method according to the Brosman method (1979). Results: it was found that cellular cardiomyoplasty significantly improves the structure of the postinfarction heart, manifests itself in a decrease in the scar area and connective tissue, respectively, in an increase in the number of vessels and the percentage of preserved muscle fibers. The best results were achieved with intramyocardial injection, which requires confirmation of this fact in a clinical study. Conclusions: cellular cardiomyoplasty with any method of introducing a cell graft has a positive effect both on the morphological substrate of the heart in the form of a decrease in the size of the scar during postinfarction remodeling, an increase in the number of newly formed vessels and an increase in the percentage of preserved cardiomyocytes. This occurs due to the homing of MSCs into the ischemic zone and the commonality of two mechanisms – direct differentiation into endothelial cells","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84064819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.15587/2519-4798.2020.220202
N. Chuiko
The aim. To determine the state of the cerebral vascular endothelium and the role of immunocompetent cells in the ischemic stroke development on the background of atherosclerosis. Materials and methods. We studied cerebral vessels of 50 deaths with ischemic cerebral infarctions, 50 – with severe cerebral atherosclerosis without CVD (cerebrovascular disease) manifestation and 50 deaths, whose cause of death was not related to CVD and atherosclerosis (control group). Histological preparations of vessels were stained with hematoxylin-eosin and Masson Trichrome, and also immunohistochemical study was conducted using CD31/PECAM-1 (Endothelial Cell Marker) Ab-1, CD4 (CD4 Ab-8), CD8 (SP 16), CD20 (CD20 Ab-1) CD68 and (CD68/Macrophage Marker Ab-4) markers. Results. Under ischemic strokes and severe atherosclerosis the cerebral vessels endothelium acquires structural changes in form of rupture, desquamation and exfoliation, formation of desquamated endothelial cells clusters. Speaking of endothelial damage, it should not be supposed that changes should occur at the macroscopic level only, endothelial damage at the cellular level shall be sufficient enough. Immunocompetent cells are of key importance in atherosclerosis development; adhesion on the luminal surface of arteries, presence of a large number of these cells under the endothelium and of more mature macrophages in the intima depth indicates the influx of these cells, which actively potentiate atherosclerosis formation, from the blood into the artery wall. Conclusions . Disorders of the endothelial lining with changes in endothelial cells morphology contribute to the atherosclerotic plaque development. Lymphocytes and macrophages form the molecular basis of many important processes, including the inflammatory response and the immune response
{"title":"Endothelium condition and role of immunocompetent cells in atherosclerosis development as a cause of ischemic stroke","authors":"N. Chuiko","doi":"10.15587/2519-4798.2020.220202","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.220202","url":null,"abstract":"The aim. To determine the state of the cerebral vascular endothelium and the role of immunocompetent cells in the ischemic stroke development on the background of atherosclerosis. Materials and methods. We studied cerebral vessels of 50 deaths with ischemic cerebral infarctions, 50 – with severe cerebral atherosclerosis without CVD (cerebrovascular disease) manifestation and 50 deaths, whose cause of death was not related to CVD and atherosclerosis (control group). Histological preparations of vessels were stained with hematoxylin-eosin and Masson Trichrome, and also immunohistochemical study was conducted using CD31/PECAM-1 (Endothelial Cell Marker) Ab-1, CD4 (CD4 Ab-8), CD8 (SP 16), CD20 (CD20 Ab-1) CD68 and (CD68/Macrophage Marker Ab-4) markers. Results. Under ischemic strokes and severe atherosclerosis the cerebral vessels endothelium acquires structural changes in form of rupture, desquamation and exfoliation, formation of desquamated endothelial cells clusters. Speaking of endothelial damage, it should not be supposed that changes should occur at the macroscopic level only, endothelial damage at the cellular level shall be sufficient enough. Immunocompetent cells are of key importance in atherosclerosis development; adhesion on the luminal surface of arteries, presence of a large number of these cells under the endothelium and of more mature macrophages in the intima depth indicates the influx of these cells, which actively potentiate atherosclerosis formation, from the blood into the artery wall. Conclusions . Disorders of the endothelial lining with changes in endothelial cells morphology contribute to the atherosclerotic plaque development. Lymphocytes and macrophages form the molecular basis of many important processes, including the inflammatory response and the immune response","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83002169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.15587/2519-4798.2020.219263
E. Klevakina, I. Anikin, O. Mykhalchuk
The aim of the research. To study the effect of invasive and non-invasive lung ventilation in full-term infants with moderate and severe HIE on cerebral perfusion and central hemodynamics.Materials and methods To study the effect of non – invasive lung ventilation on central hemodynamics and cerebral perfusion, 60 infants with moderate and severe YIE were examined, thirty of them had early tracheal extubation 72 hours after birth and were transferred to non–invasive nasal lung ventilation with intermittent positive pressure (NIPPV), another 30 new-borns formed a comparison group. They had a traditional MV.Results No differences were found between the mean blood pressure and heart rate in the newborn core and comparison group, both during the first day of stay and during the fourth day, when the core group infants were extubated and transferred to NIPPV, but the mean BP level increased significantly after the core group infants were transferred to NIPPV, compared to the values of this indicator during the first day (p<0.05).The study of central hemodynamics indices in the new-born infants of the main group within a day after extubation and transfer to NIPPV did not reveal any differences in comparison with the new-born comparison groups. Stroke volume, heart index and left ventricular ejection fraction did not differ in the main group and comparison group.When studying the effect of non-invasive lung ventilation on brain perfusion, no reliable differences between the study groups were also found.Conclusions. No statistical difference was found between НI and EF in the main group and the comparison group, respectively. Non-invasive ventilation in NIPPV mode does not affect cerebral perfusion indices compared to conventional ventilation NIPPV and can be used in intensive care of new-borns with HIE
{"title":"Effect of non–invasive ventilation on central and brain hemodynamics in term newborn infants with hypoxic – ischemic encephalopathy","authors":"E. Klevakina, I. Anikin, O. Mykhalchuk","doi":"10.15587/2519-4798.2020.219263","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.219263","url":null,"abstract":"The aim of the research. To study the effect of invasive and non-invasive lung ventilation in full-term infants with moderate and severe HIE on cerebral perfusion and central hemodynamics.Materials and methods To study the effect of non – invasive lung ventilation on central hemodynamics and cerebral perfusion, 60 infants with moderate and severe YIE were examined, thirty of them had early tracheal extubation 72 hours after birth and were transferred to non–invasive nasal lung ventilation with intermittent positive pressure (NIPPV), another 30 new-borns formed a comparison group. They had a traditional MV.Results No differences were found between the mean blood pressure and heart rate in the newborn core and comparison group, both during the first day of stay and during the fourth day, when the core group infants were extubated and transferred to NIPPV, but the mean BP level increased significantly after the core group infants were transferred to NIPPV, compared to the values of this indicator during the first day (p<0.05).The study of central hemodynamics indices in the new-born infants of the main group within a day after extubation and transfer to NIPPV did not reveal any differences in comparison with the new-born comparison groups. Stroke volume, heart index and left ventricular ejection fraction did not differ in the main group and comparison group.When studying the effect of non-invasive lung ventilation on brain perfusion, no reliable differences between the study groups were also found.Conclusions. No statistical difference was found between НI and EF in the main group and the comparison group, respectively. Non-invasive ventilation in NIPPV mode does not affect cerebral perfusion indices compared to conventional ventilation NIPPV and can be used in intensive care of new-borns with HIE","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82882497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.15587/2519-4798.2020.219945
A. Vlasov
Newborns with congenital defects usually have a burdened perinatal history, and their mothers' pregnancy proceeds against the background of severe complications and exacerbation of chronic pathology. In modern obstetrics, many indicative scales are used to determine the degree of risk of perinatal and maternal pathology, including a point assessment on the Coopland scale. The aim. Conduct a clinical and epidemiological analysis of perinatal and maternal risk factors according to the A. Coopland scale and analyze the frequency of perinatal pathology in newborns with congenital defects in the early neonatal period. Materials and methods. We retrospectively and selectively analyzed 88 birth histories of mothers of newborns with congenital malformations who received surgical treatment in the neonatal center and scored risk factors for perinatal and maternal pathology according to the A. Coopland scale. Results. It was revealed that mothers of newborns with congenital defects were included in the groups of high (3–6 points) and very high (7 or more) predicted risk of perinatal and maternal pathology. The high-risk group included 28 (32 %) mothers, and 60 women (68 %) the very high-risk group of perinatal and maternal pathology. Conclusions. In women with more than 15 points on the A. Coopland scale 4.7 times more often children were born with intrauterine infection, gastrointestinal disorders, NEC and severe respiratory disorders in the early neonatal period compared with mothers who gave birth to newborns with the same gestational age and a group of low and high risk of perinatal and maternal pathology
{"title":"Analysis of risk factors of perinatal pathology in newborns with congenital defects","authors":"A. Vlasov","doi":"10.15587/2519-4798.2020.219945","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.219945","url":null,"abstract":"Newborns with congenital defects usually have a burdened perinatal history, and their mothers' pregnancy proceeds against the background of severe complications and exacerbation of chronic pathology. In modern obstetrics, many indicative scales are used to determine the degree of risk of perinatal and maternal pathology, including a point assessment on the Coopland scale. The aim. Conduct a clinical and epidemiological analysis of perinatal and maternal risk factors according to the A. Coopland scale and analyze the frequency of perinatal pathology in newborns with congenital defects in the early neonatal period. Materials and methods. We retrospectively and selectively analyzed 88 birth histories of mothers of newborns with congenital malformations who received surgical treatment in the neonatal center and scored risk factors for perinatal and maternal pathology according to the A. Coopland scale. Results. It was revealed that mothers of newborns with congenital defects were included in the groups of high (3–6 points) and very high (7 or more) predicted risk of perinatal and maternal pathology. The high-risk group included 28 (32 %) mothers, and 60 women (68 %) the very high-risk group of perinatal and maternal pathology. Conclusions. In women with more than 15 points on the A. Coopland scale 4.7 times more often children were born with intrauterine infection, gastrointestinal disorders, NEC and severe respiratory disorders in the early neonatal period compared with mothers who gave birth to newborns with the same gestational age and a group of low and high risk of perinatal and maternal pathology","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79170600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.15587/2519-4798.2020.220010
A. Oparin, A. Titkova, K. Stepanchenko, V. Tseluyko, M. Kochuieva, O. Korzh
The aim of the work – conduct a literature review to study the most important and complex aspects in the diagnosis and treatment of gastroesophageal reflux disease that are encountered in the practice of a family doctor. Results. Gastroesophageal reflux disease is becoming an increasingly important disease for the young and ablebodied population, which gives it not only a medical, but also an economic aspect. This is one of the most common pathologies in the practice of not only a therapist, family doctor and gastroenterologist, but – due to the presence of extraesophageal manifestations – in a wider range of medical specialties. Since gastroesophageal reflux disease has many “masks”, this leads to delayed diagnosis and treatment. This disease does not have a “gold standard” in routine diagnostics, and the presence of its refractory forms leads to treatment failures and complications. With a comorbid course of gastroesophageal reflux disease, both diagnosis and treatment require an integrated approach. Conclusions. Gastroesophageal reflux disease remains one of the most common diseases in the practice of a family doctor. Despite the extensive study of this pathology, it continues to remain difficult in terms of diagnosis and treatment, which is associated with the presence of extraesophageal manifestations, comorbid and refractory course. This requires an additional study of pathogenetic mechanisms and the development of therapeutic and diagnostic measures on the basis of this
{"title":"Gastroesophageal reflux disease in patients with various comorbidal pathology","authors":"A. Oparin, A. Titkova, K. Stepanchenko, V. Tseluyko, M. Kochuieva, O. Korzh","doi":"10.15587/2519-4798.2020.220010","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.220010","url":null,"abstract":"The aim of the work – conduct a literature review to study the most important and complex aspects in the diagnosis and treatment of gastroesophageal reflux disease that are encountered in the practice of a family doctor. Results. Gastroesophageal reflux disease is becoming an increasingly important disease for the young and ablebodied population, which gives it not only a medical, but also an economic aspect. This is one of the most common pathologies in the practice of not only a therapist, family doctor and gastroenterologist, but – due to the presence of extraesophageal manifestations – in a wider range of medical specialties. Since gastroesophageal reflux disease has many “masks”, this leads to delayed diagnosis and treatment. This disease does not have a “gold standard” in routine diagnostics, and the presence of its refractory forms leads to treatment failures and complications. With a comorbid course of gastroesophageal reflux disease, both diagnosis and treatment require an integrated approach. Conclusions. Gastroesophageal reflux disease remains one of the most common diseases in the practice of a family doctor. Despite the extensive study of this pathology, it continues to remain difficult in terms of diagnosis and treatment, which is associated with the presence of extraesophageal manifestations, comorbid and refractory course. This requires an additional study of pathogenetic mechanisms and the development of therapeutic and diagnostic measures on the basis of this","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88634628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-25DOI: 10.15587/2519-4798.2020.217557
T. Litovchenko, O. Bilchenko, V. Malakhov, O. Dubenko, O. Sukhonosova, Viktoriia Bokatuieva, M. Markova, Vadym Nikonov
The aim – to determine the role of viral lesions of the central nervous system (CNS) in the development of epileptic seizures and epilepsy. Materials and methods: analysis of scientific literature data. The review discusses the role of non-epidemic viral encephalitis and human immunodeficiency virus in the development of acute epileptic seizures and epilepsy; epidemiological data and risk factors. Results. Viral infections are often complicated by acute (early) epileptic seizures and causes the risk of further epilepsy. The mechanisms of development of early and late seizures differ. Except herpetic encephalitis, in which risk of epilepsy in early seizures is up to 60 %, the risk of late seizures in other viral encephalitis hasn't been determined. Timely treatment of viral infections and early seizures reduces the risk of epilepsy. Treatment of epilepsy with infectious lesions of the central nervous system and other types of symptomatic epilepsy is similar, and the antiepileptic drug is determined by the semiology of seizures. The interaction between it and antiinfective drugs can change the concentration in the blood of each of them, which will lead to reduced effectiveness or toxicity. This is especially important for individuals with human immunodeficiency virus infection in whom antiretroviral drugs have significant interactions with antiepileptic drugs. Since epileptic activity of the brain is associated with the severity and complications of the underlying infection, early aggressive therapy prevents the development of late seizures and epilepsy. Conclusions. CNS infections account for 15 % of all newly diagnosed symptomatic epileptic seizures. The 20-year risk of unprovoked seizures and epilepsy after CNS infections ranges from 2.4 % to 22 %. The risk depends on the etiology, location of the lesion and the severity; it is high in herpes-associated encephalitis
{"title":"Analysis of the role of viral encephalitis in the development of epileptic seizures and epilepsy","authors":"T. Litovchenko, O. Bilchenko, V. Malakhov, O. Dubenko, O. Sukhonosova, Viktoriia Bokatuieva, M. Markova, Vadym Nikonov","doi":"10.15587/2519-4798.2020.217557","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.217557","url":null,"abstract":"The aim – to determine the role of viral lesions of the central nervous system (CNS) in the development of epileptic seizures and epilepsy. Materials and methods: analysis of scientific literature data. The review discusses the role of non-epidemic viral encephalitis and human immunodeficiency virus in the development of acute epileptic seizures and epilepsy; epidemiological data and risk factors. Results. Viral infections are often complicated by acute (early) epileptic seizures and causes the risk of further epilepsy. The mechanisms of development of early and late seizures differ. Except herpetic encephalitis, in which risk of epilepsy in early seizures is up to 60 %, the risk of late seizures in other viral encephalitis hasn't been determined. Timely treatment of viral infections and early seizures reduces the risk of epilepsy. Treatment of epilepsy with infectious lesions of the central nervous system and other types of symptomatic epilepsy is similar, and the antiepileptic drug is determined by the semiology of seizures. The interaction between it and antiinfective drugs can change the concentration in the blood of each of them, which will lead to reduced effectiveness or toxicity. This is especially important for individuals with human immunodeficiency virus infection in whom antiretroviral drugs have significant interactions with antiepileptic drugs. Since epileptic activity of the brain is associated with the severity and complications of the underlying infection, early aggressive therapy prevents the development of late seizures and epilepsy. Conclusions. CNS infections account for 15 % of all newly diagnosed symptomatic epileptic seizures. The 20-year risk of unprovoked seizures and epilepsy after CNS infections ranges from 2.4 % to 22 %. The risk depends on the etiology, location of the lesion and the severity; it is high in herpes-associated encephalitis","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84877557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-30DOI: 10.15587/2519-4798.2020.213824
H. Diachenko, Y. Volkova
The aim : to increase the effectiveness of treatment of patients of surgical profile with overweight by developing algorithms for perioperative intensive care for the successful implementation of the ERAS protocol. Material and methods . The basis of this study is the analysis of the results of a comprehensive clinical and instrumental dynamic examination of 122 patients with surgical herniological profile for a period of 1 day to 1 month from the date of surgery. The study included patients with ventral hernias of the anterior abdominal wall, which were determined by the SWR classification. The conditions for admission to the study under the conditions of inclusion were a fence 10 days before surgery to determine the analysis of vitamin D concentration. 3 groups of patients were identified (control, with addition to the protocol of treatment of cholecalciferol, with addition to the protocol of treatment of cholecalciferol and a solution of D-fructose-1,6-diphosphate sodium salt of hydrate). Determined the type of fat distribution, index of visceral obesity, triglycerides, high-density lipoprotein, leptin, fasting glucose, endogenous insulin, calculated the index of HOMA. Parametric statistics methods were used to process the obtained data. Results . In the vast majority of overweight patients (90 %) the abdominal type of fat distribution with the presence of visceral index obesity was determined. At the time of screening, the concentration of leptin in the blood of all studied patients exceeded the upper limit of normal by almost 4 times. The absence of a probable connection between the level of 25 (OH) D and leptin was determined, which confirms the presence of obesity due to reduced muscle mass and impaired energy metabolism, the presence of a relationship between the level of 25 (OH) D, HOMA, concentration of parathyroid hormone in the blood. Conclusions . Implementation of a planned surgical profile in overweight patients at the screening stage 10 days before surgery to determine the level of 25 (OH) D in the blood is a key point in deciding the possibility of conducting the perioperative period according to the ERAS program. Additional purpose to its classical protocol of cholecalciferol and solution of D-fructose-1,6-diphosphate sodium salt of hydrate increases the quality of motor activity of patients after surgery, increases their adaptive potential by restoring lost muscle function. The optimized classical algorithm of the ERAS-program significantly (p <0.05) improved the quality of life in the long term (30 days after surgery), such as physical functioning, general health, viability scale, mental health (SF-36 scale) and decreased body mass index
{"title":"Dynamics of leptin, insulin resistance, parathyroid hormone, 25(OH)D in the implementation of the eras-protocol in patients of surgical profile","authors":"H. Diachenko, Y. Volkova","doi":"10.15587/2519-4798.2020.213824","DOIUrl":"https://doi.org/10.15587/2519-4798.2020.213824","url":null,"abstract":"The aim : to increase the effectiveness of treatment of patients of surgical profile with overweight by developing algorithms for perioperative intensive care for the successful implementation of the ERAS protocol. Material and methods . The basis of this study is the analysis of the results of a comprehensive clinical and instrumental dynamic examination of 122 patients with surgical herniological profile for a period of 1 day to 1 month from the date of surgery. The study included patients with ventral hernias of the anterior abdominal wall, which were determined by the SWR classification. The conditions for admission to the study under the conditions of inclusion were a fence 10 days before surgery to determine the analysis of vitamin D concentration. 3 groups of patients were identified (control, with addition to the protocol of treatment of cholecalciferol, with addition to the protocol of treatment of cholecalciferol and a solution of D-fructose-1,6-diphosphate sodium salt of hydrate). Determined the type of fat distribution, index of visceral obesity, triglycerides, high-density lipoprotein, leptin, fasting glucose, endogenous insulin, calculated the index of HOMA. Parametric statistics methods were used to process the obtained data. Results . In the vast majority of overweight patients (90 %) the abdominal type of fat distribution with the presence of visceral index obesity was determined. At the time of screening, the concentration of leptin in the blood of all studied patients exceeded the upper limit of normal by almost 4 times. The absence of a probable connection between the level of 25 (OH) D and leptin was determined, which confirms the presence of obesity due to reduced muscle mass and impaired energy metabolism, the presence of a relationship between the level of 25 (OH) D, HOMA, concentration of parathyroid hormone in the blood. Conclusions . Implementation of a planned surgical profile in overweight patients at the screening stage 10 days before surgery to determine the level of 25 (OH) D in the blood is a key point in deciding the possibility of conducting the perioperative period according to the ERAS program. Additional purpose to its classical protocol of cholecalciferol and solution of D-fructose-1,6-diphosphate sodium salt of hydrate increases the quality of motor activity of patients after surgery, increases their adaptive potential by restoring lost muscle function. The optimized classical algorithm of the ERAS-program significantly (p <0.05) improved the quality of life in the long term (30 days after surgery), such as physical functioning, general health, viability scale, mental health (SF-36 scale) and decreased body mass index","PeriodicalId":21672,"journal":{"name":"ScienceRise: Medical Science","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82970130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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