The popularity of poultry meat is growing in almost all countries. The consumer prefers chicken considering its high nutritional and dietary value, relatively low price, safety, and the absence of religious restrictions. At the same time, close attention is paid to the safety of food products since food poisoning in poultry processing enterprises is an urgent problem. Based on the results of research on 25 poultry carcasses intended for sale in retail chains, L. monocytogenes was detected in chicken carcasses, broiler chickens in 1 case (4.0 % of studies), semi-finished products from broiler chickens in 2 cases (8 % of studies). During the study of 45 samples in the primary processing shop to establish possible sources and areas of cross-insemination of L. monocytogenes on the surface of poultry carcasses, in 8 cases or 17.8 % of the studies, L. monocytogenes was isolated in washings from carcasses, from the water of the cooling bath ‒ in 3 cases from 13 or 23.1 % of studies, in washes from equipment ‒ in 1 case or 14.3 %, in washes from hands ‒ in 1 case (14.3 %). The resistance of L. monocytogenes to the action of the disinfectant “Balandas-A Forte” 0.07 % solution has been studied; it has a non-permanent antimicrobial effect against L. monocytogenes, 0.15 % ‒ ensures the inactivation of L. monocytogenes in a bath with a water temperature of 10 ºС. There are no significant differences in the organoleptic parameters of the meat and the broth (transparency, aroma), made from poultry meat cooled in the usual way and when using the disinfectant “Balandez-A Forte.” However, in the treated carcasses, compared to the control, there is a change in the color of the surface of the carcasses and internal fat - their color becomes yellow and has a wax-like layering, which the consumer can negatively perceive, the reason for this may be the effect of peracetic acid (PAA) on the adipose tissue of the bird. According to physical and chemical indicators, poultry meat cooled with cold water and using a disinfectant solution met the requirements of the standards. Disinfectant “Balandez-A Forte” of 0.07 and 0.15 % concentration inactivates L. monocytogenes, 0.07 % solution reduces KMAFAnM by three orders of magnitude from (1.7 ± 0.3)×104 to (3.1 ± 0.8)×101, but does not inactivate bacteria of the group of Escherichia coli, water from a cooling bath with a working solution of 0.15 % concentration of a disinfectant allows inactivation of BGKP and ensures inactivation of L. monocytogenes in cold water (10 ºC).
{"title":"Contamination of broiler chicken carcasses with L. monocytogenes and the effect of disinfectants on organoleptic and physicochemical parameters of meat","authors":"N. V. Tyshkivskaya, L. G. Bartkiv, M. Tyshkivsky","doi":"10.32718/nvlvet11204","DOIUrl":"https://doi.org/10.32718/nvlvet11204","url":null,"abstract":"The popularity of poultry meat is growing in almost all countries. The consumer prefers chicken considering its high nutritional and dietary value, relatively low price, safety, and the absence of religious restrictions. At the same time, close attention is paid to the safety of food products since food poisoning in poultry processing enterprises is an urgent problem. Based on the results of research on 25 poultry carcasses intended for sale in retail chains, L. monocytogenes was detected in chicken carcasses, broiler chickens in 1 case (4.0 % of studies), semi-finished products from broiler chickens in 2 cases (8 % of studies). During the study of 45 samples in the primary processing shop to establish possible sources and areas of cross-insemination of L. monocytogenes on the surface of poultry carcasses, in 8 cases or 17.8 % of the studies, L. monocytogenes was isolated in washings from carcasses, from the water of the cooling bath ‒ in 3 cases from 13 or 23.1 % of studies, in washes from equipment ‒ in 1 case or 14.3 %, in washes from hands ‒ in 1 case (14.3 %). The resistance of L. monocytogenes to the action of the disinfectant “Balandas-A Forte” 0.07 % solution has been studied; it has a non-permanent antimicrobial effect against L. monocytogenes, 0.15 % ‒ ensures the inactivation of L. monocytogenes in a bath with a water temperature of 10 ºС. There are no significant differences in the organoleptic parameters of the meat and the broth (transparency, aroma), made from poultry meat cooled in the usual way and when using the disinfectant “Balandez-A Forte.” However, in the treated carcasses, compared to the control, there is a change in the color of the surface of the carcasses and internal fat - their color becomes yellow and has a wax-like layering, which the consumer can negatively perceive, the reason for this may be the effect of peracetic acid (PAA) on the adipose tissue of the bird. According to physical and chemical indicators, poultry meat cooled with cold water and using a disinfectant solution met the requirements of the standards. Disinfectant “Balandez-A Forte” of 0.07 and 0.15 % concentration inactivates L. monocytogenes, 0.07 % solution reduces KMAFAnM by three orders of magnitude from (1.7 ± 0.3)×104 to (3.1 ± 0.8)×101, but does not inactivate bacteria of the group of Escherichia coli, water from a cooling bath with a working solution of 0.15 % concentration of a disinfectant allows inactivation of BGKP and ensures inactivation of L. monocytogenes in cold water (10 ºC).","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"51 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Y. Krempa, O. Kozenko, B. Gutyj, I. V. Dvyliuk, N. Magrelo, H. V. Sus, V. V. Voroniak, A. O. Vysotskyi, U. Vus, T. Martyshuk
Inconsistency in housing and feeding conditions, production stress, a combination of environmental factors, and technological solutions reduce the protective properties of the animal body, which often leads to a decrease in their productive properties. The work aimed to investigate changes in indicators of the humoral protection factor, in particular, the quantitative composition of immunoglobulins M, G, and A in the body of young pigs under the influence of immunostimulating agents Globigen® Pig Doser and Globigen® Jump Start, which include specific Ig Y. To perform this task in the conditions of the private farm of FG PE "Glynyany Agro" of Lviv region, Zolochiv district, which uses a single-phase method of raising pigs, a study was conducted with the involvement of 36 piglets-analogues of crossbreeds of the Great White and Landrace breeds, which were formed into three groups of 12 animals each. Piglets of the control group were fed feed of a standard diet. The piglets of the first experimental group were orally administered 2 ml/g of Globigen®Pig Doser during the first three days after birth. Then, according to the technology, they consumed the feed prescribed in the diet. The piglets of the second experimental group were given Globigen®Jump Start, which was mixed with the feed of the basic ration in the amount of 2 kg per ton of feed and, starting from the age of 7 days, was fed to the piglets according to the technology. It was established that the use of immunostimulating agents was characterized by an increase in Ig G and Ig A in the blood serum of piglets in the most critical period of their lives. Thus, in the blood serum of 30-day-old piglets under the influence of Globigen® Pig Doser, with a high level of probability (Р < 0.01), an increase of this class of antibodies to the level of 13.47 mg/ml was detected, with the use of Globigen® Jump Start, this indicator reached the mark 12.51 mg/ml. Since this period, colostral immunity, represented by placental immunoglobulins of class G and colostrum immunoglobulins of class A, is already exhausted; we assume that the formation of the body's immune response of young pigs has begun. We believe that the increase in the content of class A immunoglobulins, most likely the secretory form of the piglets themselves, when using Globigen® Jump Start, which reached the mark of 1.48 mg/ml, indicates the formation of immune resistance mechanisms. The effectiveness, specificity, and mechanism of action of these agents is based on the ability of their components, particularly Ig Y, to bind to pathogens or their fragments, to neutralize and safely remove them from the body, which helps to increase the barrier function of the intestinal mucosa. The obtained research results confirm the practicality of using Globigen® Pig Doser and Globigen® Jump Start immunostimulants during single-phase rearing of piglets to increase the level of non-specific resistance and obtain healthy young animals with a high level of v
{"title":"The dynamics of the content of immunoglobulins in the blood serum of piglets according to the actions of immunostimulating agents","authors":"N. Y. Krempa, O. Kozenko, B. Gutyj, I. V. Dvyliuk, N. Magrelo, H. V. Sus, V. V. Voroniak, A. O. Vysotskyi, U. Vus, T. Martyshuk","doi":"10.32718/nvlvet11208","DOIUrl":"https://doi.org/10.32718/nvlvet11208","url":null,"abstract":"Inconsistency in housing and feeding conditions, production stress, a combination of environmental factors, and technological solutions reduce the protective properties of the animal body, which often leads to a decrease in their productive properties. The work aimed to investigate changes in indicators of the humoral protection factor, in particular, the quantitative composition of immunoglobulins M, G, and A in the body of young pigs under the influence of immunostimulating agents Globigen® Pig Doser and Globigen® Jump Start, which include specific Ig Y. To perform this task in the conditions of the private farm of FG PE \"Glynyany Agro\" of Lviv region, Zolochiv district, which uses a single-phase method of raising pigs, a study was conducted with the involvement of 36 piglets-analogues of crossbreeds of the Great White and Landrace breeds, which were formed into three groups of 12 animals each. Piglets of the control group were fed feed of a standard diet. The piglets of the first experimental group were orally administered 2 ml/g of Globigen®Pig Doser during the first three days after birth. Then, according to the technology, they consumed the feed prescribed in the diet. The piglets of the second experimental group were given Globigen®Jump Start, which was mixed with the feed of the basic ration in the amount of 2 kg per ton of feed and, starting from the age of 7 days, was fed to the piglets according to the technology. It was established that the use of immunostimulating agents was characterized by an increase in Ig G and Ig A in the blood serum of piglets in the most critical period of their lives. Thus, in the blood serum of 30-day-old piglets under the influence of Globigen® Pig Doser, with a high level of probability (Р < 0.01), an increase of this class of antibodies to the level of 13.47 mg/ml was detected, with the use of Globigen® Jump Start, this indicator reached the mark 12.51 mg/ml. Since this period, colostral immunity, represented by placental immunoglobulins of class G and colostrum immunoglobulins of class A, is already exhausted; we assume that the formation of the body's immune response of young pigs has begun. We believe that the increase in the content of class A immunoglobulins, most likely the secretory form of the piglets themselves, when using Globigen® Jump Start, which reached the mark of 1.48 mg/ml, indicates the formation of immune resistance mechanisms. The effectiveness, specificity, and mechanism of action of these agents is based on the ability of their components, particularly Ig Y, to bind to pathogens or their fragments, to neutralize and safely remove them from the body, which helps to increase the barrier function of the intestinal mucosa. The obtained research results confirm the practicality of using Globigen® Pig Doser and Globigen® Jump Start immunostimulants during single-phase rearing of piglets to increase the level of non-specific resistance and obtain healthy young animals with a high level of v","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"100 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic kidney disease (CKD) in cats is a long-term process characterized by the irreversible loss of metabolic, endocrine, and excretory functions of the kidneys, usually due to the development of nephrosclerosis. The overall prevalence of CKD in cats is approximately 2 % to 4 % and increases to 30–40 % in cats older than 10 years. It is the second leading cause of death. CKD can occur due to polycystic disease, pyelonephritis, glomerulonephritis, neoplasia, amyloidosis, viral infections, urolithiasis and is the most common cause of hypertension in cats, accounting for 20 to 60 % of cases. Cats often have a combined course of hyperthyroidism and CKD. For CKD in cats, hyporexia (64.8 %), polyuria and polydipsia (56.7 %), anemia of mucous membranes (67.5 %), impaired coordination of movements (35 %), vomiting (43 %), ulcerative stomatitis were most often noted (37.8 %). Polyuria, polydipsia, polyphagia, nausea, and vomiting were detected in cats with combined course of hyperthyroidism. It was established that the 1st stage of CKD passes without pronounced changes in the blood serum but with the preservation of the symptoms of the disease. The concentration of cystatin C in the blood serum of cats with impaired kidney function at all stages was higher (P > 0.001) compared to clinically healthy animals by 13.9, 34.7, and 73.6 %. In cats at II-IV stages, a significant increase in azotemia was established, accompanied by an increase in creatinine and urea content (P > 0.001) compared to clinically healthy animals. A clear tendency to decrease the rate of glomerular filtration was established at different stages of CKD: at II – 115.8 ± 1.43 ml/min/1.73; the third – 101.7 ± 1.02; IV – 83.9 ± 1.20 ml/min/1.73. The level of SDMA in the blood serum of cats was higher (P < 0.001) in stage II (21.30 ± 0.64 μg/dl), III (30.96 ± 1.17 μg/dl), and IV (91.87 ± 12.54 μg/dL) compared to the control group. A violation of mineral and bone metabolism was established, even in the early stages of the disease, which was characterized by a decrease (Р < 0.001) in the level of total calcium and an increase in the level of inorganic phosphorus and potassium in the blood serum of cats. In the combined course of CKD and hyperthyroidism, an increase (Р < 0.001) in the level of the total hormone T4 in blood serum was found in diseased cats in stages II and IV. Arterial hypertension was established in 16 cats, the risk of which increased from moderate (148 ± 4.7/98 ± 3.8 mmHg at the II stage of the course) to high at the IV stage (188 ± 3.8 mmHg. and 116.7 ± 4.06 mm Hg). The development of anemic syndrome, microcytic hyperchromic anemia, and changes in the physical properties and chemical composition of urine were established.
{"title":"Prevalence and features of early diagnosis of chronic kidney disease in cats","authors":"O. Y. Ostrovskyi, L. Slivinska","doi":"10.32718/nvlvet11216","DOIUrl":"https://doi.org/10.32718/nvlvet11216","url":null,"abstract":"Chronic kidney disease (CKD) in cats is a long-term process characterized by the irreversible loss of metabolic, endocrine, and excretory functions of the kidneys, usually due to the development of nephrosclerosis. The overall prevalence of CKD in cats is approximately 2 % to 4 % and increases to 30–40 % in cats older than 10 years. It is the second leading cause of death. CKD can occur due to polycystic disease, pyelonephritis, glomerulonephritis, neoplasia, amyloidosis, viral infections, urolithiasis and is the most common cause of hypertension in cats, accounting for 20 to 60 % of cases. Cats often have a combined course of hyperthyroidism and CKD. For CKD in cats, hyporexia (64.8 %), polyuria and polydipsia (56.7 %), anemia of mucous membranes (67.5 %), impaired coordination of movements (35 %), vomiting (43 %), ulcerative stomatitis were most often noted (37.8 %). Polyuria, polydipsia, polyphagia, nausea, and vomiting were detected in cats with combined course of hyperthyroidism. It was established that the 1st stage of CKD passes without pronounced changes in the blood serum but with the preservation of the symptoms of the disease. The concentration of cystatin C in the blood serum of cats with impaired kidney function at all stages was higher (P > 0.001) compared to clinically healthy animals by 13.9, 34.7, and 73.6 %. In cats at II-IV stages, a significant increase in azotemia was established, accompanied by an increase in creatinine and urea content (P > 0.001) compared to clinically healthy animals. A clear tendency to decrease the rate of glomerular filtration was established at different stages of CKD: at II – 115.8 ± 1.43 ml/min/1.73; the third – 101.7 ± 1.02; IV – 83.9 ± 1.20 ml/min/1.73. The level of SDMA in the blood serum of cats was higher (P < 0.001) in stage II (21.30 ± 0.64 μg/dl), III (30.96 ± 1.17 μg/dl), and IV (91.87 ± 12.54 μg/dL) compared to the control group. A violation of mineral and bone metabolism was established, even in the early stages of the disease, which was characterized by a decrease (Р < 0.001) in the level of total calcium and an increase in the level of inorganic phosphorus and potassium in the blood serum of cats. In the combined course of CKD and hyperthyroidism, an increase (Р < 0.001) in the level of the total hormone T4 in blood serum was found in diseased cats in stages II and IV. Arterial hypertension was established in 16 cats, the risk of which increased from moderate (148 ± 4.7/98 ± 3.8 mmHg at the II stage of the course) to high at the IV stage (188 ± 3.8 mmHg. and 116.7 ± 4.06 mm Hg). The development of anemic syndrome, microcytic hyperchromic anemia, and changes in the physical properties and chemical composition of urine were established.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"134 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Trukhanovych, M. Kukhtyn, Y. Perkiy, N. Boltyk, V. Klymyk, T. Rushchynska, B. Tykhonova
Bacteriocins, in particular, nisin, are increasingly used for the development of sanitizing agents along with natural antimicrobial substances, such as organic acids, essential oils, alcohols, and others. The purpose of the work was to investigate the bactericidal effect of nisin on test cultures of microorganisms that cause mastitis in cows for its use in means for pre-milking treatment of the udder in combination with lactic acid. The minimum inhibitory concentration was studied on test cultures of Staphylococcus aureus (ATCC 25923), Escherichia coli (055К59 №3912/41) and Streptococcus uberis (field strain) by the method of serial dilutions with exposures of 30 seconds and 10 minutes. In the experiment was used nisin produced by Glory Chem Co., Ltd. (China), lactic acid food 80 % solution (“Klebrig”, Belgium). It was established that the minimum inhibitory concentration of nisin after exposure for 30 seconds on S. aureus cultures was 1.0 % and Str. uberis – 0.125 %. Nisin at the content of 2 % and 1 % in the solution did not show the minimum inhibitory concentration on E. coli cultures within 30 seconds, but inhibited 91.7 and 82.3 % of the microflora, respectively, at this concentration. It was found that the minimum inhibitory concentration of lactic acid after exposure for 30 seconds on S. aureus and E. coli cultures was 2 %, and on Str. uberis – 1 %. At the same time, lactic acid diluted below the minimum inhibitory concentration, in particular, 1 % and 0.5 %, inhibited 98.9–96.5 % of microorganisms in the solution, which may be sufficient for its use in means for processing the udder of cows before milking and at lower concentration. The study of the bactericidal effect by the well method showed that nisin exhibits a synergistic bactericidal effect with lactic acid and can be used in combination to create a means for pre-milking treatment of the udder of cows. Therefore, in order to create means for pre-milking treatment of the skin of udders of cows, it is necessary that the working concentration of nisin be from 0.5 to 1.0 %, and lactic acid from 1 to 2 %.
{"title":"The prospect of using nisin for pre-milking treatment of the udder of cows","authors":"T. Trukhanovych, M. Kukhtyn, Y. Perkiy, N. Boltyk, V. Klymyk, T. Rushchynska, B. Tykhonova","doi":"10.32718/nvlvet11207","DOIUrl":"https://doi.org/10.32718/nvlvet11207","url":null,"abstract":"Bacteriocins, in particular, nisin, are increasingly used for the development of sanitizing agents along with natural antimicrobial substances, such as organic acids, essential oils, alcohols, and others. The purpose of the work was to investigate the bactericidal effect of nisin on test cultures of microorganisms that cause mastitis in cows for its use in means for pre-milking treatment of the udder in combination with lactic acid. The minimum inhibitory concentration was studied on test cultures of Staphylococcus aureus (ATCC 25923), Escherichia coli (055К59 №3912/41) and Streptococcus uberis (field strain) by the method of serial dilutions with exposures of 30 seconds and 10 minutes. In the experiment was used nisin produced by Glory Chem Co., Ltd. (China), lactic acid food 80 % solution (“Klebrig”, Belgium). It was established that the minimum inhibitory concentration of nisin after exposure for 30 seconds on S. aureus cultures was 1.0 % and Str. uberis – 0.125 %. Nisin at the content of 2 % and 1 % in the solution did not show the minimum inhibitory concentration on E. coli cultures within 30 seconds, but inhibited 91.7 and 82.3 % of the microflora, respectively, at this concentration. It was found that the minimum inhibitory concentration of lactic acid after exposure for 30 seconds on S. aureus and E. coli cultures was 2 %, and on Str. uberis – 1 %. At the same time, lactic acid diluted below the minimum inhibitory concentration, in particular, 1 % and 0.5 %, inhibited 98.9–96.5 % of microorganisms in the solution, which may be sufficient for its use in means for processing the udder of cows before milking and at lower concentration. The study of the bactericidal effect by the well method showed that nisin exhibits a synergistic bactericidal effect with lactic acid and can be used in combination to create a means for pre-milking treatment of the udder of cows. Therefore, in order to create means for pre-milking treatment of the skin of udders of cows, it is necessary that the working concentration of nisin be from 0.5 to 1.0 %, and lactic acid from 1 to 2 %.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"38 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There are only 15 % of water reservoirs in Ukraine that can be used as sources of water supply, taking into account water treatment facilities. 55 % of polluted objects and 30 % very dirty, water from these sources cannot be used even after cleaning. With the help of sanitary-hygienic, ecological and water management assessment, they ensure the quality of water in the impact on the environment and the quality of fish products. The main documents that determine the quality, properties and chemical composition of drinking water are DSTU 7525:2014 and DSTU 27384:2005, they specify the main toxicological and organoleptic concentrations of chemicals that must be contained in water. The standard “Sources of centralized economic drinking water supply, hygienic and ecological requirements for water quality and selection rules” makes it possible to develop a complex of interrelated standards and norms, including the use, protection and restoration of water resources, objective assessment of the ecological state and quality of surface and underground waters and their compliance with the requirements of EU standards. Water treatment equipment and technologies used nowadays for wastewater treatment do not provide a sufficient degree of purification and disinfection. Surface water reservoirs, along with impurities of natural origin, contain chemical pollution – heavy metals, pesticides, insecticides, oil products, phenols. The methods of wastewater disinfection are divided into chemical, physical, physico-chemical and in the conditions of artificial and natural biocenoses.
{"title":"Hygienic assessment of water quality and safety according to national requirements","authors":"I. I. Panikar, V. Rud, N. Vartik","doi":"10.32718/nvlvet11209","DOIUrl":"https://doi.org/10.32718/nvlvet11209","url":null,"abstract":"There are only 15 % of water reservoirs in Ukraine that can be used as sources of water supply, taking into account water treatment facilities. 55 % of polluted objects and 30 % very dirty, water from these sources cannot be used even after cleaning. With the help of sanitary-hygienic, ecological and water management assessment, they ensure the quality of water in the impact on the environment and the quality of fish products. The main documents that determine the quality, properties and chemical composition of drinking water are DSTU 7525:2014 and DSTU 27384:2005, they specify the main toxicological and organoleptic concentrations of chemicals that must be contained in water. The standard “Sources of centralized economic drinking water supply, hygienic and ecological requirements for water quality and selection rules” makes it possible to develop a complex of interrelated standards and norms, including the use, protection and restoration of water resources, objective assessment of the ecological state and quality of surface and underground waters and their compliance with the requirements of EU standards. Water treatment equipment and technologies used nowadays for wastewater treatment do not provide a sufficient degree of purification and disinfection. Surface water reservoirs, along with impurities of natural origin, contain chemical pollution – heavy metals, pesticides, insecticides, oil products, phenols. The methods of wastewater disinfection are divided into chemical, physical, physico-chemical and in the conditions of artificial and natural biocenoses.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"88 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138606181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to study the morphological and biochemical parameters of the blood of lambs affected by the causative agent of eimeria, an experimental group of animals of the Romanov breed, two months old, was formed. The lambs were spontaneously infected with the causative agent of eimeria, which was confirmed by laboratory and clinical studies. The second group of animals was free from eimeria and was kept separately. According to the results of blood tests of sick lambs, a decrease in the number of peripheral blood erythrocytes by 29.66 %, an increase in the total number of leukocytes by 47.12 % compared to the blood parameters of the control group of animals was revealed. A change in leukogram parameters was also noted: an increase in the number of eosinophils by 55.43 %, young neutrophils by 75 %. At the same time, there was a decrease in the number of rod-shaped neutrophils and an increase in the number of segmented neutrophils by 8.7 and 29.51 %, respectively. A decrease in the number of lymphocytes and monocytes by 31.68 and 12 %, respectively, was registered. Biochemical changes in the blood serum of sick lambs also underwent changes. A decrease in the content of total protein by 36.06 %, albumin by 13.09 % was established. An increase in the content of globulins and dysproteinemia was noted. Hemoglobin content was also reduced compared to blood parameters of healthy lambs by 32.25 %, as well as glucose content by 27.83 %. The activity level of ALT and AST increased by 43.43 and 33.15 %, respectively, as well as a slight decrease in GGT activity. The difference was statistically significant (Р < 0.01 and Р < 0.05). Differences in the blood parameters of sick lambs have been established and indicate a syndrome of the body's general inflammatory reaction, anemia, damage to the alimentary canal by the causative agent of eimeria and intoxication.
为了研究艾美耳球虫致病菌对羔羊血液形态和生化参数的影响，采用2月龄的罗曼诺夫(Romanov)品种作为实验动物。经实验室和临床研究证实，羔羊自发感染了艾美耳球虫病原体。第二组无艾美耳球虫，单独饲养。根据病羔羊的血液检查结果，与对照组相比，外周血红细胞数量减少了29.66%，白细胞总数增加了47.12%。白细胞参数的变化也被注意到:嗜酸性粒细胞增加了55.43%，年轻中性粒细胞增加了75%。同时，杆状中性粒细胞的数量减少了8.7%，节段性中性粒细胞的数量增加了29.51%。淋巴细胞和单核细胞的数量分别减少了31.68%和12%。病羔羊的血清生化变化也发生了变化。总蛋白含量降低36.06%，白蛋白含量降低13.09%。注意到球蛋白和蛋白异常血症的含量增加。与健康羔羊的血液参数相比，血红蛋白含量降低了32.25%，葡萄糖含量降低了27.83%。谷丙转氨酶和谷草转氨酶活性分别升高43.43%和33.15%，而谷草转氨酶活性略有下降。差异有统计学意义(Р < 0.01， Р < 0.05)。患病羔羊血液参数的差异已经确定，这表明身体的一般炎症反应、贫血、艾美拉球虫病原体对消化道的损害和中毒的综合征。
{"title":"Changes in peripheral blood parameters of lambs with spontaneous eimeriosis","authors":"O. V. Filipenko","doi":"10.32718/nvlvet11212","DOIUrl":"https://doi.org/10.32718/nvlvet11212","url":null,"abstract":"In order to study the morphological and biochemical parameters of the blood of lambs affected by the causative agent of eimeria, an experimental group of animals of the Romanov breed, two months old, was formed. The lambs were spontaneously infected with the causative agent of eimeria, which was confirmed by laboratory and clinical studies. The second group of animals was free from eimeria and was kept separately. According to the results of blood tests of sick lambs, a decrease in the number of peripheral blood erythrocytes by 29.66 %, an increase in the total number of leukocytes by 47.12 % compared to the blood parameters of the control group of animals was revealed. A change in leukogram parameters was also noted: an increase in the number of eosinophils by 55.43 %, young neutrophils by 75 %. At the same time, there was a decrease in the number of rod-shaped neutrophils and an increase in the number of segmented neutrophils by 8.7 and 29.51 %, respectively. A decrease in the number of lymphocytes and monocytes by 31.68 and 12 %, respectively, was registered. Biochemical changes in the blood serum of sick lambs also underwent changes. A decrease in the content of total protein by 36.06 %, albumin by 13.09 % was established. An increase in the content of globulins and dysproteinemia was noted. Hemoglobin content was also reduced compared to blood parameters of healthy lambs by 32.25 %, as well as glucose content by 27.83 %. The activity level of ALT and AST increased by 43.43 and 33.15 %, respectively, as well as a slight decrease in GGT activity. The difference was statistically significant (Р < 0.01 and Р < 0.05). Differences in the blood parameters of sick lambs have been established and indicate a syndrome of the body's general inflammatory reaction, anemia, damage to the alimentary canal by the causative agent of eimeria and intoxication.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"48 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the work was to establish the effectiveness of the application of a complex reproductive protocol to improve the reproductive capacity of females. The essence of the protocol consists in a combination of monitoring and treatment and preventive measures, which are carried out from the moment of planning the pregnancy of the females dogs until birth and the first weeks after birth, which are combined into a complex reproductive protocol. The protocol includes three stages (preparation for pregnancy; management of the reproductive females dogs during pregnancy; postpartum period). The experiment was conducted on 25 bull terrier female (Canis familiaris) of different ages. The effectiveness of the application of a complex reproductive protocol in the breeding of bull terrier female was experimentally established which is characterized by an increase in the efficiency of insemination, the weight of the nest and the viability of the puppies. In particular, the efficiency of fertilization of female was 92 % (against 72 % in the control group). Using a complex reproductive protocol, the viability of the resulting puppies was slightly higher than that of the female of the research group. In particular, 84.7 % of puppies obtained in the group following the Protocol had high indicators of viability (APGAR – 7–10 points), which is 6.9 % more than the indicators in the control group of animals. Also, in the experimental group, 9.3 % of animals had average APGAR scores (4–6 points), and only 5.9 % of puppies had a low degree of viability (APGAR – 0–3 points). Regarding multifertility, in the control group of female, most female (33.3 %) had 5 pups each, while 22.1 % had 6–7 pups in the nest, 27.8 % of animals had 2–3 pups each. In addition, one females had one puppy. The conducted tests of the Protocol established an increase in the fertility of females. Thus, 62.5 % of the female of the experimental group had from 5 to 7 puppies, which is 6.9 % more than the indicators of the female of the control group. In addition, only one female from the experimental group had 2 and 3 puppies, respectively, and there was no female with one born puppy. Therefore, the effectiveness of the application of a complex reproductive protocol in the breeding of bull terrier female, which is characterized by an increase in the efficiency of insemination, litter mass and viability of puppies, has been established.
{"title":"New approaches to improving the reproductive function of females dogs","authors":"V. I. Forkun, O. M. Bobryts’ka","doi":"10.32718/nvlvet11222","DOIUrl":"https://doi.org/10.32718/nvlvet11222","url":null,"abstract":"The aim of the work was to establish the effectiveness of the application of a complex reproductive protocol to improve the reproductive capacity of females. The essence of the protocol consists in a combination of monitoring and treatment and preventive measures, which are carried out from the moment of planning the pregnancy of the females dogs until birth and the first weeks after birth, which are combined into a complex reproductive protocol. The protocol includes three stages (preparation for pregnancy; management of the reproductive females dogs during pregnancy; postpartum period). The experiment was conducted on 25 bull terrier female (Canis familiaris) of different ages. The effectiveness of the application of a complex reproductive protocol in the breeding of bull terrier female was experimentally established which is characterized by an increase in the efficiency of insemination, the weight of the nest and the viability of the puppies. In particular, the efficiency of fertilization of female was 92 % (against 72 % in the control group). Using a complex reproductive protocol, the viability of the resulting puppies was slightly higher than that of the female of the research group. In particular, 84.7 % of puppies obtained in the group following the Protocol had high indicators of viability (APGAR – 7–10 points), which is 6.9 % more than the indicators in the control group of animals. Also, in the experimental group, 9.3 % of animals had average APGAR scores (4–6 points), and only 5.9 % of puppies had a low degree of viability (APGAR – 0–3 points). Regarding multifertility, in the control group of female, most female (33.3 %) had 5 pups each, while 22.1 % had 6–7 pups in the nest, 27.8 % of animals had 2–3 pups each. In addition, one females had one puppy. The conducted tests of the Protocol established an increase in the fertility of females. Thus, 62.5 % of the female of the experimental group had from 5 to 7 puppies, which is 6.9 % more than the indicators of the female of the control group. In addition, only one female from the experimental group had 2 and 3 puppies, respectively, and there was no female with one born puppy. Therefore, the effectiveness of the application of a complex reproductive protocol in the breeding of bull terrier female, which is characterized by an increase in the efficiency of insemination, litter mass and viability of puppies, has been established.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"15 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139187309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. M. Broshkov, O. Bezaltychna, A. P. Kytaeva, V. M. Yasko
The production of beekeeping plays a crucial role in the population's nutrition. Bees yield valuable dietary products, such as honey, and provide raw materials for the food industry, such as beeswax. In addition to these primary products, bees produce royal jelly, flower pollen, propolis, homogenate of drone larvae, and bee venom. In regions with advanced agriculture, bees are utilized to pollinate entomophilous crops. Bee pollination enhances plants' similarity and yield, improving agricultural product quality. Honey-producing bees consume pollen, with flower pollen from nectariferous plants serving as the raw material, followed by bee bread, which is brought into the hive. However, the agroclimatic conditions in various regions of Ukraine only sometimes provide sufficient diversity of nectariferous plants to adequately meet the protein and mineral nutrition needs of bees. Minerals in animal organisms perform crucial functions, providing structural integrity to the skeleton and constituting essential components of organic compounds, vitamins, and enzymatic systems. In contemporary animal production, heavy metals threaten the health of both humans and animals. According to established norms, many of these metals are vital elements that must be included in the animal diet. Inadequate or excessive levels of these elements in the animal body can lead to the inactivation of enzymatic systems, impaired reproduction, decreased productivity, and reduced product quality. Heavy metals, especially mercury, lead, chromium, and nickel, are more dangerous toxins in living organisms at elevated concentrations. The chemical composition of living organisms and their impact on geochemical processes varies. To ensure normal vitality and the production of high-quality animal products, animals must be provided with a balanced diet and an optimal content of minerals that contribute to increased productivity and production efficiency. Therefore, studying the content of heavy metals such as iron, copper, zinc, lead, cobalt, and cadmium in honey obtained under different agroecological conditions in Ukraine is of significant economic importance. The research results indicate that the content of heavy metals in honey from the Western region, except iron and zinc, was higher than in the Southern region. Specifically, for copper, this advantage amounted to 86.725 mg/kg or 1.4 times, for lead – 50.203 mg/kg or 2.8 times, for cobalt – 57.865 mg/kg or 24.2 times, and for cadmium – 4.663 mg/kg or 2.7 times. Honey from the Southern region had a higher iron content of 0.237 mg/kg or 1.1 times, and zinc by 4.412 mg/kg or 5.5 times compared to honey from the Western region. Based on the obtained data, it is established that honey from the Western region has a higher content of heavy metals than honey from the Southern region, indicating more significant contamination of nectariferous plants in the Western region.
{"title":"Content of proline and heavy metals in beekeeping prod ucts of different agro-ecological conditions of Ukraine","authors":"M. M. Broshkov, O. Bezaltychna, A. P. Kytaeva, V. M. Yasko","doi":"10.32718/nvlvet11219","DOIUrl":"https://doi.org/10.32718/nvlvet11219","url":null,"abstract":"The production of beekeeping plays a crucial role in the population's nutrition. Bees yield valuable dietary products, such as honey, and provide raw materials for the food industry, such as beeswax. In addition to these primary products, bees produce royal jelly, flower pollen, propolis, homogenate of drone larvae, and bee venom. In regions with advanced agriculture, bees are utilized to pollinate entomophilous crops. Bee pollination enhances plants' similarity and yield, improving agricultural product quality. Honey-producing bees consume pollen, with flower pollen from nectariferous plants serving as the raw material, followed by bee bread, which is brought into the hive. However, the agroclimatic conditions in various regions of Ukraine only sometimes provide sufficient diversity of nectariferous plants to adequately meet the protein and mineral nutrition needs of bees. Minerals in animal organisms perform crucial functions, providing structural integrity to the skeleton and constituting essential components of organic compounds, vitamins, and enzymatic systems. In contemporary animal production, heavy metals threaten the health of both humans and animals. According to established norms, many of these metals are vital elements that must be included in the animal diet. Inadequate or excessive levels of these elements in the animal body can lead to the inactivation of enzymatic systems, impaired reproduction, decreased productivity, and reduced product quality. Heavy metals, especially mercury, lead, chromium, and nickel, are more dangerous toxins in living organisms at elevated concentrations. The chemical composition of living organisms and their impact on geochemical processes varies. To ensure normal vitality and the production of high-quality animal products, animals must be provided with a balanced diet and an optimal content of minerals that contribute to increased productivity and production efficiency. Therefore, studying the content of heavy metals such as iron, copper, zinc, lead, cobalt, and cadmium in honey obtained under different agroecological conditions in Ukraine is of significant economic importance. The research results indicate that the content of heavy metals in honey from the Western region, except iron and zinc, was higher than in the Southern region. Specifically, for copper, this advantage amounted to 86.725 mg/kg or 1.4 times, for lead – 50.203 mg/kg or 2.8 times, for cobalt – 57.865 mg/kg or 24.2 times, and for cadmium – 4.663 mg/kg or 2.7 times. Honey from the Southern region had a higher iron content of 0.237 mg/kg or 1.1 times, and zinc by 4.412 mg/kg or 5.5 times compared to honey from the Western region. Based on the obtained data, it is established that honey from the Western region has a higher content of heavy metals than honey from the Southern region, indicating more significant contamination of nectariferous plants in the Western region.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"72 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. O. Medvid, Zh. O. Peredera, N. S. Shcherbakova, S. B. Peredera
In the areas of the European Union, which are traditionally considered honey producers, we are observing a decrease in nectar resources and a decrease in the number of bee colonies due to anthropogenic factors, the destructive influence of pesticides and other chemical compounds, the development of new parasitocenoses, climate changes and experimental phenomena accompanying these changes, according to FAO, the population bees decreased by 37 %. Due to climate changes, honey production is decreasing in Italy. Honey consumption in the European Union exceeds production with self-sufficiency by 60 %. In 2021, the leading honey suppliers were Ukraine (which supplies 30 % of EU imports) and China (almost 28 % of EU imports). Comparing the data with 2020, we can note the increase in imports from China by 30 %). Since 2015, the European Commission has been monitoring the European honey market, noting a significant percentage of fraud and falsification of honey sold in the EU. Among the imported batches of honey in the EU in 2021 and 2022, almost every second (46 %) is suspected of adulteration, according to the results of “From the Hives” Joint Research Center (JRC) of the European Commission. Honey for sale must comply with Directive UE 2001/110/CE. In the coordinated joint actions of the EU, called “Dagli alveari” by 15 EU member states, Switzerland, and the Netherlands, 320 samples of honey originating from 20 exporting countries were selected. The research aimed to establish the presence of sugar syrups of exogenous origin to increase the volume to obtain more profit. Out of 320 examined samples, 147 (46 %) turned out to be falsified; their indicators did not meet the Directive UE 2001/110/CE, and accordingly, they are not “natural honey”. Falsified batches of honey were imported from Ukraine and China (66 out of 89 is 74 %), and honey originating from Turkey (14 out of 15 is 93 %). Honey imported from Great Britain has the highest adulteration rate (10 out of 10, 100 %). In Italy, a comprehensive action plan has been introduced to prevent the falsification of honey. The new control plan should include a quantitative increase in samples for research and the use of more effective tests to detect falsification: nuclear magnetic resonance (RMN) and high-performance liquid chromatography (HPLC). Honey produced in Italy must have the inscription “Italian honey” on the label, while honey originating from the countries of the European Union must contain the inscription “mixture of honey originating from the EU countries”, indicating the names of the producing countries. If honey comes from non-EU countries, it should be written “a mixture of honeys from non-EU countries” with the name of the producer country added.
{"title":"Analysis of the Italian honey market","authors":"O. O. Medvid, Zh. O. Peredera, N. S. Shcherbakova, S. B. Peredera","doi":"10.32718/nvlvet11202","DOIUrl":"https://doi.org/10.32718/nvlvet11202","url":null,"abstract":"In the areas of the European Union, which are traditionally considered honey producers, we are observing a decrease in nectar resources and a decrease in the number of bee colonies due to anthropogenic factors, the destructive influence of pesticides and other chemical compounds, the development of new parasitocenoses, climate changes and experimental phenomena accompanying these changes, according to FAO, the population bees decreased by 37 %. Due to climate changes, honey production is decreasing in Italy. Honey consumption in the European Union exceeds production with self-sufficiency by 60 %. In 2021, the leading honey suppliers were Ukraine (which supplies 30 % of EU imports) and China (almost 28 % of EU imports). Comparing the data with 2020, we can note the increase in imports from China by 30 %). Since 2015, the European Commission has been monitoring the European honey market, noting a significant percentage of fraud and falsification of honey sold in the EU. Among the imported batches of honey in the EU in 2021 and 2022, almost every second (46 %) is suspected of adulteration, according to the results of “From the Hives” Joint Research Center (JRC) of the European Commission. Honey for sale must comply with Directive UE 2001/110/CE. In the coordinated joint actions of the EU, called “Dagli alveari” by 15 EU member states, Switzerland, and the Netherlands, 320 samples of honey originating from 20 exporting countries were selected. The research aimed to establish the presence of sugar syrups of exogenous origin to increase the volume to obtain more profit. Out of 320 examined samples, 147 (46 %) turned out to be falsified; their indicators did not meet the Directive UE 2001/110/CE, and accordingly, they are not “natural honey”. Falsified batches of honey were imported from Ukraine and China (66 out of 89 is 74 %), and honey originating from Turkey (14 out of 15 is 93 %). Honey imported from Great Britain has the highest adulteration rate (10 out of 10, 100 %). In Italy, a comprehensive action plan has been introduced to prevent the falsification of honey. The new control plan should include a quantitative increase in samples for research and the use of more effective tests to detect falsification: nuclear magnetic resonance (RMN) and high-performance liquid chromatography (HPLC). Honey produced in Italy must have the inscription “Italian honey” on the label, while honey originating from the countries of the European Union must contain the inscription “mixture of honey originating from the EU countries”, indicating the names of the producing countries. If honey comes from non-EU countries, it should be written “a mixture of honeys from non-EU countries” with the name of the producer country added.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"46 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. I. Boychuk, V. I. Karpovskyi, B. Gutyj, I. Hryshchuk, V. I. Karpovskyi, A. Hryshchuk
In the regulation of homeostasis, the tone of the autonomic nervous system plays a key role, in the regulation of the metabolism of organic substances, which will affect the indicators of the fatty acid composition of the blood. As a result, the profile of saturated fatty acids can affect the nutritional value of dairy products. According to the results of a chromatographic study, the influence of the tone of the autonomic nervous system on the content of saturated fatty acids: caproic, capric, lauric, myristic, pentadecanoic, palmitic, and arachinic fatty acids was established. Caproic acid relative to the experimental group of normotonics has high indicators in sympathotonic 1.42 ± 0.06 (Р ≤ 0.001) and vagotonic 0.71 ± 0.08 (Р ≤ 0.001). Capric acid is 76 % (Р ≤ 0.001) lower in the content of animals with vagotonia and 9 % (Р ≤ 0.05) in goats with sympathotonia compared to normotonia. Lauric acid in the experimental group of vagotonics is 84 % (Р ≤ 0.001) more and 22 % (Р ≤ 0.05) less in sympathotonics compared to normotonics. Compared to animals with normotonia, myristic acid has high values in goats with sympathotonia by 22 % (Р ≤ 0.05) and with vagotonia by 82 % (Р ≤ 0.001). Pentadecanoic acid is 66 % less (Р ≤ 0.05) in sympathotonics and 29 % more in vagotonics (Р ≤ 0.01) compared to the experimental group of normotonic. Compared to goats with normotonia, palmitic acid has high indicators of animals with vagotonia by 6 % (Р ≤ 0.001) and low indicators with sympathotonia by 4 % (Р ≤ 0.05). Arachidonic acid in sympathotonic had the highest content by 38 % (Р ≤ 0.001) in comparison with normotonic. Considering the total content of saturated fatty acids in the blood plasma of goats during the chromatographic study, a regularity was established. The experimental group of vagotonic had the highest levels of fatty acids among other animals. Sympathotonic drugs mostly had a lower percentage content.
{"title":"The content of saturated fatty acids in the plasma of goats depending on the tone of the autonomic nervous system","authors":"B. I. Boychuk, V. I. Karpovskyi, B. Gutyj, I. Hryshchuk, V. I. Karpovskyi, A. Hryshchuk","doi":"10.32718/nvlvet11206","DOIUrl":"https://doi.org/10.32718/nvlvet11206","url":null,"abstract":"In the regulation of homeostasis, the tone of the autonomic nervous system plays a key role, in the regulation of the metabolism of organic substances, which will affect the indicators of the fatty acid composition of the blood. As a result, the profile of saturated fatty acids can affect the nutritional value of dairy products. According to the results of a chromatographic study, the influence of the tone of the autonomic nervous system on the content of saturated fatty acids: caproic, capric, lauric, myristic, pentadecanoic, palmitic, and arachinic fatty acids was established. Caproic acid relative to the experimental group of normotonics has high indicators in sympathotonic 1.42 ± 0.06 (Р ≤ 0.001) and vagotonic 0.71 ± 0.08 (Р ≤ 0.001). Capric acid is 76 % (Р ≤ 0.001) lower in the content of animals with vagotonia and 9 % (Р ≤ 0.05) in goats with sympathotonia compared to normotonia. Lauric acid in the experimental group of vagotonics is 84 % (Р ≤ 0.001) more and 22 % (Р ≤ 0.05) less in sympathotonics compared to normotonics. Compared to animals with normotonia, myristic acid has high values in goats with sympathotonia by 22 % (Р ≤ 0.05) and with vagotonia by 82 % (Р ≤ 0.001). Pentadecanoic acid is 66 % less (Р ≤ 0.05) in sympathotonics and 29 % more in vagotonics (Р ≤ 0.01) compared to the experimental group of normotonic. Compared to goats with normotonia, palmitic acid has high indicators of animals with vagotonia by 6 % (Р ≤ 0.001) and low indicators with sympathotonia by 4 % (Р ≤ 0.05). Arachidonic acid in sympathotonic had the highest content by 38 % (Р ≤ 0.001) in comparison with normotonic. Considering the total content of saturated fatty acids in the blood plasma of goats during the chromatographic study, a regularity was established. The experimental group of vagotonic had the highest levels of fatty acids among other animals. Sympathotonic drugs mostly had a lower percentage content.","PeriodicalId":21703,"journal":{"name":"Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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