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Evaluation of an Efficient and Skin-Adherent Semisolid Sunscreen Nanoformulation 一种高效皮肤粘附型半固体防晒纳米配方的评价
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-06-15 DOI: 10.1159/000525176
L. Frank, R. P. Gazzi, A. Pohlmann, S. Guterres, R. V. Contri
Introduction: Sunscreens are substances applied on the skin surface to protect the skin from the harmful effects of UV light. Nanoparticles can increase the retention time of the sunscreen on the skin surface and its efficacy, by acting as physical barriers. The present investigation aimed to evaluate the influence of the chitosan coating of benzophenone-3-loaded lipid-core nanocapsules (CH-LCN) on the skin adhesion and photoprotective effect of the sunscreen. Methods: CH-LNC were obtained by the interfacial deposition of preformed polymer. A suitable semisolid formulation was obtained by using hydroxyethyl cellulose as the gel-forming polymer. Skin adhesion experiments were performed in vitro by applying the formulation on porcine skin and keeping it under water at 32 °C for up to 60 min. Photoprotective effect was analyzed in vitro by the capacity of the formulations to protect a photo unstable substance (resveratrol) from degradation under UV light. Results: CH-LNC presented size of around 150 nm, with low polydispersity, positive zeta potential, due to chitosan, and benzophenone-3 encapsulation efficiency of close to 100% (3 mg/mL). The proposed gel presented suitable consistence and pH for skin application and benzophenone-3 concentration of around 3 mg/g. Although coated and uncoated lipid-core nanocapsules increased benzophenone-3 skin adhesion after 10 min of water immersion, only the nanoparticles coated with chitosan were able to do so after 60 min. The chitosan coating of the nanocapsules increased the photoprotection of the sunscreen under UVA and UVB light after 60 min of exposure, probably due to the film-forming properties of chitosan. Conclusion: The chitosan coating of CH-LCN increased the skin adhesion and the photoprotective effect of the sunscreen.
简介:防晒霜是涂在皮肤表面的物质,保护皮肤免受紫外线的有害影响。纳米粒子可以作为物理屏障,增加防晒霜在皮肤表面的停留时间和功效。本实验旨在研究壳聚糖包被负载二苯甲酮-3脂核纳米胶囊(CH-LCN)对防晒霜皮肤粘附性和光防护效果的影响。方法:采用预成型聚合物界面沉积法制备CH-LNC。以羟乙基纤维素为成胶聚合物,得到了合适的半固体配方。在体外进行皮肤粘附实验,将该配方涂于猪皮肤上,并将其置于32°C的水中长达60分钟。通过该配方在体外保护光不稳定物质(白藜芦醇)免受紫外线降解的能力来分析其光保护作用。结果:CH-LNC粒径约为150 nm,多分散性低,zeta电位正,壳聚糖包封率接近100% (3 mg/mL)。该凝胶具有适合皮肤使用的浓度和pH,二苯甲酮-3浓度约为3mg /g。虽然包被和未包被的脂核纳米胶囊在浸泡10分钟后都能增加二苯甲酮-3的皮肤粘附性,但在60分钟后,只有包被壳聚糖的纳米胶囊才能增加二苯甲酮-3的皮肤粘附性。在暴露60分钟后,壳聚糖包被的纳米胶囊增加了防晒霜在UVA和UVB光下的光防护,这可能是由于壳聚糖的成膜特性。结论:壳聚糖包被CH-LCN增强了皮肤粘附力和防晒剂的光防护作用。
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引用次数: 2
Comparison of Lipid Foam Cream and Basic Cream on Epidermal Reconstruction in Mild Atopic Eczema 脂质泡沫霜与基础霜在轻度特应性湿疹表皮重建中的比较
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-06-15 DOI: 10.1159/000525283
D. Dähnhardt, S. Dähnhardt-Pfeiffer, Judith Schulte-Walter, Eckhard Hanisch, Thomas Neubourg, R. Fölster-Holst
Introduction: Basic therapy is of central importance in the treatment of atopic eczema. Using electron microscopic images, the morphology of epidermal skin barrier and its lipids was investigated after application of a lipid foam cream and basic cream. Methods: Patients with two contralateral comparable atopic eczema (local SCORAD 1–10) on the forearms were tested. Eczema was treated with a lipid foam cream or basic cream twice daily for 28 days. At the beginning, after 14 days, and at the end of application, the local SCORAD, trans-epidermal water loss (TEWL), skin hydration, intercellular lipid length in the intercellular space of the stratum corneum (SC), and skin lipids were determined. Results: After application of the foam cream, the epidermal skin barrier could be completely restored and corresponded to healthy skin, while the epidermal skin barrier could not reach this state after care with the basic cream. The content of lipids in the SC increases significantly by 31% after basic cream treatment, whereas they are significantly increased by 85% after application of the lipid foam cream. The local SCORAD improved for both treatments to about the same extent, and no significant results could be shown for TEWL and skin hydration. Conclusion: In subjects with mild atopic eczema, the lipid foam cream leads to a measurable recovery of the skin barrier which is much more pronounced in comparison to the basic cream.
引言:基础治疗是治疗特应性湿疹的核心。利用电镜图像,研究了脂质泡沫霜和基础霜应用后表皮皮肤屏障及其脂质的形态。方法:对前臂有两个对侧可比特应性湿疹(局部SCORAD 1-10)的患者进行测试。湿疹用脂质泡沫霜或基础霜治疗,每天两次,持续28天。在开始时、14天后和施用结束时,测定局部SCORAD、经表皮水分损失(TEWL)、皮肤水合作用、角质层细胞间隙中的细胞间脂质长度(SC)和皮肤脂质。结果:使用泡沫霜后,表皮皮肤屏障可以完全恢复,与健康皮肤相对应,而使用基础霜护理后表皮皮肤屏障不能达到这种状态。SC中的脂质含量在基础乳膏处理后显著增加31%,而在施用脂质泡沫乳膏后显著增加85%。两种治疗的局部SCORAD改善程度大致相同,TEWL和皮肤水合作用没有显著结果。结论:在轻度特应性湿疹受试者中,脂质泡沫霜可使皮肤屏障明显恢复,与基础霜相比,这一点要明显得多。
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引用次数: 0
The Emerging Therapeutic Targets for Scar Management: Genetic and Epigenetic Landscapes 疤痕管理的新兴治疗靶点:遗传和表观遗传景观
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-06-13 DOI: 10.1159/000524990
Sara Amjadian, S. Moradi, P. Mohammadi
Background: Wound healing is a complex process including hemostasis, inflammation, proliferation, and remodeling during which an orchestrated array of biological and molecular events occurs to promote skin regeneration. Abnormalities in each step of the wound healing process lead to reparative rather than regenerative responses, thereby driving the formation of cutaneous scar. Patients suffering from scars represent serious health problems such as contractures, functional and esthetic concerns as well as painful, thick, and itchy complications, which generally decrease the quality of life and impose high medical costs. Therefore, therapies reducing cutaneous scarring are necessary to improve patients’ rehabilitation. Summary: Current approaches to remove scars, including surgical and nonsurgical methods, are not efficient enough, which is in principle due to our limited knowledge about underlying mechanisms of pathological as well as the physiological wound healing process. Thus, therapeutic interventions focused on basic science including genetic and epigenetic knowledge are recently taken into consideration as promising approaches for scar management since they have the potential to provide targeted therapies and improve the conventional treatments as well as present opportunities for combination therapy. In this review, we highlight the recent advances in skin regenerative medicine through genetic and epigenetic approaches to achieve novel insights for the development of safe, efficient, and reproducible therapies and discuss promising approaches for scar management. Key Message: Genetic and epigenetic regulatory switches are promising targets for scar management, provided the associated challenges are to be addressed.
背景:伤口愈合是一个复杂的过程,包括止血、炎症、增殖和重塑,在此过程中,会发生一系列生物和分子事件来促进皮肤再生。伤口愈合过程中每一步的异常都会导致修复反应,而不是再生反应,从而导致皮肤疤痕的形成。患有疤痕的患者代表着严重的健康问题,如挛缩、功能和美观问题,以及疼痛、粘稠和瘙痒的并发症,这些并发症通常会降低生活质量并带来高昂的医疗成本。因此,减少皮肤疤痕的治疗对于改善患者的康复是必要的。总结:目前去除疤痕的方法,包括手术和非手术方法,不够有效,原则上是由于我们对病理和生理伤口愈合过程的潜在机制了解有限。因此,专注于基础科学(包括遗传和表观遗传学知识)的治疗干预措施最近被认为是疤痕管理的有前途的方法,因为它们有潜力提供靶向治疗,改善传统治疗,并为联合治疗提供机会。在这篇综述中,我们强调了皮肤再生医学的最新进展,通过遗传和表观遗传学方法,为开发安全、高效和可重复的疗法获得了新的见解,并讨论了有前景的疤痕管理方法。关键信息:遗传和表观遗传学调控开关是疤痕管理的有希望的靶点,前提是相关的挑战有待解决。
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引用次数: 5
Dexamethasone, a Synthetic Glucocorticoid, Induces the Activity of Androgen Receptor in Human Dermal Papilla Cells 合成糖皮质激素地塞米松诱导人真皮乳头细胞雄激素受体活性的研究
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-05-13 DOI: 10.1159/000525067
M. Kwack, O. Ben Hamida, M. Kim, Jung Chul Kim, Y. Sung
Psychosocial stress stimulates the secretion of glucocorticoids (GCs), which are stress-related neurohormones. GCs are secreted from hair follicles and promote hair follicle regression by inducing cellular apoptosis. Moreover, the androgen receptor (AR) is abundant in the balding scalp, and androgens suppress hair growth by binding to AR in androgenetic alopecia. First, by using immunofluorescence, we investigated whether the treatment of dermal papilla (DP) cells with dexamethasone (DEX), a synthetic GC, causes the translocation of the glucocorticoid receptor (GR) into the nucleus. DEX treatment causes the translocation of the GR into the nucleus. Next, we investigated whether stress-induced GCs affect the AR, a key factor in male pattern baldness. In this study, we first assessed that DEX increases the expression of AR mRNA in non-balding DP cells, which rarely express AR without androgen. RU486, a GR antagonist, attenuated DEX-inducible AR mRNA expression and AR activation in human non-balding DP cells. In addition, AR translocated into the nucleus after DEX treatment. Furthermore, we indeed showed that the expression of AR was induced in the nucleus by DEX in DP cells of human and mouse hair follicles. Our results first suggest that stress-associated hair loss may be due to increased AR expression and activity induced by DEX. These results demonstrate that hair loss occurs in non-balding scalps with low AR expression.
心理社会压力刺激糖皮质激素(GC)的分泌,这是一种与压力相关的神经激素。GC从毛囊分泌,并通过诱导细胞凋亡促进毛囊退化。此外,雄激素受体(AR)在脱发的头皮中含量丰富,雄激素通过与AR结合来抑制雄激素性脱发的头发生长。首先,通过使用免疫荧光,我们研究了用地塞米松(DEX)(一种合成的GC)处理毛乳头(DP)细胞是否会导致糖皮质激素受体(GR)易位到细胞核中。DEX治疗导致GR易位进入细胞核。接下来,我们研究了应激诱导的GC是否影响AR,AR是男性型秃发的关键因素。在这项研究中,我们首先评估了DEX增加了非秃头DP细胞中AR mRNA的表达,而在没有雄激素的情况下,这些细胞很少表达AR。RU486是一种GR拮抗剂,可减弱DEX诱导的人非脱发DP细胞中AR mRNA的表达和AR的激活。此外,在DEX处理后,AR转移到细胞核中。此外,我们确实表明,在人类和小鼠毛囊的DP细胞中,DEX在细胞核中诱导了AR的表达。我们的研究结果首先表明,应激相关的脱发可能是由于DEX诱导的AR表达和活性增加。这些结果表明,脱发发生在AR表达低的非脱发头皮中。
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引用次数: 2
Front & Back Matter 正面和背面
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-05-01 DOI: 10.1159/000524836
J. Fluhr, M. Lane
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引用次数: 0
A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing 肝细胞生长因子-和5α-二氢睾酮明胶微球与脂肪来源干细胞的复合物增强伤口愈合
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-04-19 DOI: 10.1159/000524188
K. Tao, X. Bai, P. Ji, Yue Zhang, Tao Cao, F. Han, Zhi Zhang, Hao Guan, D. Hu
Introduction: Reconstructing sebaceous glands is one goal of functionally healing patients who have suffered severe burns, instead of the simple pursuit of wound closure. Effective regeneration of skin appendages remains a challenge in skin wound management and research. Objective: The aim of this study was to evaluate the differentiation of adipose-derived stem cells (ADSCs) into sebaceous glands and clarified the involvement of hepatocyte growth factor (HGF) and 5α-dihydrotestosterone (5α-DHT) in this process. Methods: This study used HGF- and 5α-DHT-gelatin microspheres to treat human ADSCs and investigated the reconstruction of sebaceous glands. HGF- and 5α-DHT-gelatin microspheres were constructed using microcapsule slow-release technology. A mice full-thickness skin-wound model was established to evaluate wound healing, and hematoxylin-eosin staining was utilized to determine the skin structure. Results: In vitro analyses found that HGF- and 5α-DHT-gelatin microspheres promoted migration of and tube formation by ADSCs. Furthermore, AKT/ERK signaling, which is related to sebocyte and sweat gland epithelial-cell growth, was activated after HGF and 5α-DHT treatment. An in vivo wound healing model demonstrated that ADSCs primed with amnion-loaded HGF- and 5α-DHT-gelatin microspheres promoted wound healing and increased sebaceous gland formation compared to the control group. Conclusions: This study confirms the efficacy of ADSCs treated with amnion and HGF- and 5α-DHT-gelatin microspheres in accelerating wound healing and effectively restoring sebaceous glands. This engineered tissue provides insight into and a novel therapeutic material for burns and full-thickness skin wounds.
简介:重建皮脂腺是功能性治愈严重烧伤患者的目标之一,而不是简单地追求伤口闭合。皮肤附属物的有效再生仍然是皮肤伤口管理和研究中的一个挑战。目的:本研究旨在评估脂肪干细胞(ADSCs)向皮脂腺的分化,并阐明肝细胞生长因子(HGF)和5α-二氢睾酮(5α-DHT)在这一过程中的作用。方法:采用HGF-和5α-DHT明胶微球治疗人ADSCs,并研究其对皮脂腺的重建作用。采用微胶囊缓释技术构建了HGF-和5α-DHT明胶微球。建立小鼠全层皮肤伤口模型以评估伤口愈合,并使用苏木精-伊红染色来确定皮肤结构。结果:体外分析发现,HGF-和5α-DHT明胶微球促进了ADSCs的迁移和成管。此外,与皮脂细胞和汗腺上皮细胞生长有关的AKT/ERK信号传导在HGF和5α-DHT处理后被激活。体内伤口愈合模型表明,与对照组相比,用羊膜负载的HGF-和5α-DHT明胶微球引发的ADSCs促进了伤口愈合并增加了皮脂腺的形成。结论:本研究证实了羊膜和HGF-和5α-DHT明胶微球治疗ADSCs在加速伤口愈合和有效修复皮脂腺方面的疗效。这种工程组织为烧伤和全层皮肤伤口提供了深入了解和新型治疗材料。
{"title":"A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing","authors":"K. Tao, X. Bai, P. Ji, Yue Zhang, Tao Cao, F. Han, Zhi Zhang, Hao Guan, D. Hu","doi":"10.1159/000524188","DOIUrl":"https://doi.org/10.1159/000524188","url":null,"abstract":"Introduction: Reconstructing sebaceous glands is one goal of functionally healing patients who have suffered severe burns, instead of the simple pursuit of wound closure. Effective regeneration of skin appendages remains a challenge in skin wound management and research. Objective: The aim of this study was to evaluate the differentiation of adipose-derived stem cells (ADSCs) into sebaceous glands and clarified the involvement of hepatocyte growth factor (HGF) and 5α-dihydrotestosterone (5α-DHT) in this process. Methods: This study used HGF- and 5α-DHT-gelatin microspheres to treat human ADSCs and investigated the reconstruction of sebaceous glands. HGF- and 5α-DHT-gelatin microspheres were constructed using microcapsule slow-release technology. A mice full-thickness skin-wound model was established to evaluate wound healing, and hematoxylin-eosin staining was utilized to determine the skin structure. Results: In vitro analyses found that HGF- and 5α-DHT-gelatin microspheres promoted migration of and tube formation by ADSCs. Furthermore, AKT/ERK signaling, which is related to sebocyte and sweat gland epithelial-cell growth, was activated after HGF and 5α-DHT treatment. An in vivo wound healing model demonstrated that ADSCs primed with amnion-loaded HGF- and 5α-DHT-gelatin microspheres promoted wound healing and increased sebaceous gland formation compared to the control group. Conclusions: This study confirms the efficacy of ADSCs treated with amnion and HGF- and 5α-DHT-gelatin microspheres in accelerating wound healing and effectively restoring sebaceous glands. This engineered tissue provides insight into and a novel therapeutic material for burns and full-thickness skin wounds.","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":"35 1","pages":"206 - 214"},"PeriodicalIF":2.7,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44572427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Induction of Radiodermatitis in Nude Mouse Model Using Gamma Irradiator IBL 637 γ辐照剂ibl637诱导裸鼠放射性皮炎
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-04-13 DOI: 10.1159/000524596
T. Bernhardt, S. Kriesen, K. Manda, Christin Schlie, R. Panzer, G. Hildebrandt, B. Vollmar, S. Emmert, L. Boeckmann
Introduction: Acute radiodermatitis is a common, though severe, side effect of radiotherapy against cancer that may lead to an interruption or even abortion of the radiotherapy. Mouse models provide an excellent tool to study pathomechanisms of a radiation-induced dermatitis as well as to test and develop novel innovative treatment strategies. Objective: The aim of this study was to provide an overview of different mouse models and irradiation devices that have been used so far and to describe the process of the induction of a radiation dermatitis in an immune proficient nude mouse model (SKH1-Hrhr) using a IBL 637 cesium-137γ-ray machine. Methods: This process includes the construction of a radiation shielding chamber, restricting the radiation to the right hind leg of the mouse, a dosimetry, and a dose finding study to identify the appropriate irradiation dose to induce a moderate radiation dermatitis. Results: A radiation shielding chamber was successfully constructed allowing selective irradiation of the right hind leg. A moderate radiodermatitis is induced with irradiation doses in the range of 60–70 Gy under the here described conditions. Symptoms peak about 8 days after irradiation and decrease relatively quickly thereafter. Histological analyses confirmed typical signs of inflammation. Conclusion: This study describes for the first time a protocol to induce a moderate radiodermatitis in the nude mouse model SKH1-Hrhr using a IBL 637 gamma irradiator. This protocol will allow researchers to study novel treatment strategies to alleviate the burden of a radiodermatitis as a side effect of cancer treatment.
简介:急性放射性皮炎是放疗治疗癌症时常见但严重的副作用,可能导致放疗中断甚至流产。小鼠模型为研究辐射性皮炎的病理机制以及测试和开发新的创新治疗策略提供了一个很好的工具。目的:本研究的目的是概述迄今为止使用的不同小鼠模型和照射装置,并描述使用IBL 637铯-137γ射线机诱导免疫熟练裸鼠模型(SKH1-Hrhr)辐射皮炎的过程。方法:建立辐射屏蔽室,将辐射限制在小鼠右后肢,进行剂量测定和剂量测定研究,以确定诱导中度放射性皮炎的适当辐照剂量。结果:成功构建了右后腿选择性照射的辐射屏蔽室。在这里描述的条件下,用60-70戈瑞范围内的辐照剂量诱发中度放射性皮炎。照射后约8天症状达到高峰,此后症状减轻较快。组织学分析证实了典型的炎症症状。结论:本研究首次描述了一种使用IBL 637 γ辐照剂诱导裸鼠模型SKH1-Hrhr中度放射性皮炎的方案。该方案将使研究人员能够研究新的治疗策略,以减轻放射性皮炎作为癌症治疗副作用的负担。
{"title":"Induction of Radiodermatitis in Nude Mouse Model Using Gamma Irradiator IBL 637","authors":"T. Bernhardt, S. Kriesen, K. Manda, Christin Schlie, R. Panzer, G. Hildebrandt, B. Vollmar, S. Emmert, L. Boeckmann","doi":"10.1159/000524596","DOIUrl":"https://doi.org/10.1159/000524596","url":null,"abstract":"Introduction: Acute radiodermatitis is a common, though severe, side effect of radiotherapy against cancer that may lead to an interruption or even abortion of the radiotherapy. Mouse models provide an excellent tool to study pathomechanisms of a radiation-induced dermatitis as well as to test and develop novel innovative treatment strategies. Objective: The aim of this study was to provide an overview of different mouse models and irradiation devices that have been used so far and to describe the process of the induction of a radiation dermatitis in an immune proficient nude mouse model (SKH1-Hrhr) using a IBL 637 cesium-137γ-ray machine. Methods: This process includes the construction of a radiation shielding chamber, restricting the radiation to the right hind leg of the mouse, a dosimetry, and a dose finding study to identify the appropriate irradiation dose to induce a moderate radiation dermatitis. Results: A radiation shielding chamber was successfully constructed allowing selective irradiation of the right hind leg. A moderate radiodermatitis is induced with irradiation doses in the range of 60–70 Gy under the here described conditions. Symptoms peak about 8 days after irradiation and decrease relatively quickly thereafter. Histological analyses confirmed typical signs of inflammation. Conclusion: This study describes for the first time a protocol to induce a moderate radiodermatitis in the nude mouse model SKH1-Hrhr using a IBL 637 gamma irradiator. This protocol will allow researchers to study novel treatment strategies to alleviate the burden of a radiodermatitis as a side effect of cancer treatment.","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":"35 1","pages":"224 - 234"},"PeriodicalIF":2.7,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46015270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Correlation of Body Mass Index with Epidermal Biophysical Properties Varies with Gender in Chinese 中国人体重指数与表皮生物物理特性的相关性因性别而异
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-04-04 DOI: 10.1159/000524295
L. Ye, Q. Lai, S. Wen, Xiaohua Wang, Bin Yang, M. Man
Background: Epidermal function is associated with diabetes and renal disease. Whether obesity can reflect the changes in epidermal function is not clear yet. Objective: We assessed here the correlation of epidermal functions with body mass index (BMI) in a large Chinese cohort. Methods and Subjects: A total of 1,405 Chinese aged 21–98 years old were enrolled in this study. Epidermal functions, including transepidermal water loss (TEWL), stratum corneum hydration, and skin surface pH, were measured on the flexor forearm and the shin. Subjects’ height and body weight were also measured. Results: Age positively correlated with both TEWL and skin surface pH, while it negatively correlated with stratum corneum hydration on both the forearm and the shin of females. Similarly, age positively correlated with skin surface pH, while negatively correlating with stratum corneum hydration on both the forearm and the shin of males. In females, BMI positively correlated with skin surface pH, while it negatively correlated with stratum corneum hydration on both the forearm and the shin. However, BMI correlated neither with skin surface pH on both the forearm and the shin nor with stratum corneum hydration on the shin of males. Conclusion: These results demonstrate that correlations of BMI with age and epidermal functions vary with gender.
背景:表皮功能与糖尿病和肾脏疾病有关。肥胖是否能反映表皮功能的变化尚不清楚。目的:我们在一个大型中国队列中评估了表皮功能与体重指数(BMI)的相关性。方法和受试者:本研究共招募了1405名年龄在21-98岁之间的中国人。测量前臂屈肌和胫骨的表皮功能,包括经表皮失水(TEWL)、角质层水合作用和皮肤表面pH值。还测量了受试者的身高和体重。结果:女性的年龄与TEWL和皮肤表面pH呈正相关,而与前臂和胫骨的角质层水合作用呈负相关。同样,年龄与男性前臂和胫骨的皮肤表面pH呈正相关,而与角质层水合作用呈负相关。在女性中,BMI与皮肤表面pH呈正相关,而与前臂和胫骨的角质层水合作用呈负相关。然而,BMI既与前臂和胫骨的皮肤表面pH值无关,也与男性胫骨的角质层水合作用无关。结论:这些结果表明,BMI与年龄和表皮功能的相关性因性别而异。
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引用次数: 8
LncRNA XIST Engages in Psoriasis via Sponging miR-338-5p to Regulate Keratinocyte Proliferation and Inflammation LncRNA-XIST通过启动miR-338-5p调节角质形成细胞增殖和炎症参与银屑病
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-03-01 DOI: 10.1159/000523781
Yitao Wang, Feifei Jiang, Fang Chen, Dapeng Zhang, Jian Wang
Introduction: Psoriasis is an immune-mediated polygenic inflammatory skin disease in which keratinocyte proliferation is an important mechanism. The study investigated the role and regulatory relationship between lncRNA XIST and miR-338-5p in psoriatic patients and cell models. Methods: Serum samples were collected from 55 psoriasis patients. HaCaT was recruited for the cell experiments, and induced by M5 cytokines to mimic psoriasis in vitro. XIST and miR-338-5p levels were detected via qRT-PCR. Cell viability under different treatments was evaluated using CCK-8. ELISA was applied to measure the concentration of inflammatory cytokines. The regulatory relationship was confirmed using luciferase reporter gene assay. Results: Serum XIST was elevated in patients with psoriasis and can distinguish the psoriasis patients from healthy controls according to the receiver operating characteristic curve. A high level of XIST was positively correlated with the PASI score and serum tumor necrosis factor-alpha (TNF-α), interleukin-17A [IL-17A], and IL-22 concentrations in psoriasis patients. XIST silencing suppressed M5-induced keratinocyte proliferation and restrained the discharge of inflammatory cytokines (TNF-α, IL-17A, IL-22) and chemokines (CXCL1, CXCL8, CCL20). XIST can sponge miR-338-5p, and miR-338-5p downregulation abolished the inhibitory effect of XIST silencing on cell proliferation and inflammation. miR-338-5p was highly expressed in the clinical serum samples from psoriasis patients. The target relationship between miR-338-5p and IL-6 was proved. Conclusion: LncRNA XIST is highly expressed in the serum of patients with psoriasis, and was positively correlated with disease severity and inflammation. XIST may regulate keratinocyte proliferation and inflammation via regulating miR-338-5p/IL-6 axis.
银屑病是一种免疫介导的多基因炎症性皮肤病,角质细胞增殖是其重要机制。本研究探讨lncRNA XIST与miR-338-5p在银屑病患者及细胞模型中的作用及调控关系。方法:采集55例银屑病患者血清标本。我们招募HaCaT进行细胞实验,并通过M5细胞因子诱导,在体外模拟银屑病。通过qRT-PCR检测XIST和miR-338-5p水平。采用CCK-8检测不同处理下细胞活力。ELISA法检测炎症因子浓度。荧光素酶报告基因测定证实了两者之间的调控关系。结果:银屑病患者血清XIST水平升高,根据受试者工作特征曲线可将银屑病患者与健康对照区分开。高水平的XIST与银屑病患者PASI评分及血清肿瘤坏死因子-α (TNF-α)、白细胞介素- 17a [IL-17A]、IL-22浓度呈正相关。沉默XIST可抑制m5诱导的角化细胞增殖,抑制炎症因子(TNF-α、IL-17A、IL-22)和趋化因子(CXCL1、CXCL8、CCL20)的分泌。XIST可“海绵”miR-338-5p, miR-338-5p下调可消除XIST沉默对细胞增殖和炎症的抑制作用。miR-338-5p在银屑病患者的临床血清样本中高表达。证实了miR-338-5p与IL-6的靶标关系。结论:LncRNA XIST在银屑病患者血清中高表达,且与病情严重程度、炎症程度呈正相关。XIST可能通过调节miR-338-5p/IL-6轴调控角质细胞增殖和炎症。
{"title":"LncRNA XIST Engages in Psoriasis via Sponging miR-338-5p to Regulate Keratinocyte Proliferation and Inflammation","authors":"Yitao Wang, Feifei Jiang, Fang Chen, Dapeng Zhang, Jian Wang","doi":"10.1159/000523781","DOIUrl":"https://doi.org/10.1159/000523781","url":null,"abstract":"Introduction: Psoriasis is an immune-mediated polygenic inflammatory skin disease in which keratinocyte proliferation is an important mechanism. The study investigated the role and regulatory relationship between lncRNA XIST and miR-338-5p in psoriatic patients and cell models. Methods: Serum samples were collected from 55 psoriasis patients. HaCaT was recruited for the cell experiments, and induced by M5 cytokines to mimic psoriasis in vitro. XIST and miR-338-5p levels were detected via qRT-PCR. Cell viability under different treatments was evaluated using CCK-8. ELISA was applied to measure the concentration of inflammatory cytokines. The regulatory relationship was confirmed using luciferase reporter gene assay. Results: Serum XIST was elevated in patients with psoriasis and can distinguish the psoriasis patients from healthy controls according to the receiver operating characteristic curve. A high level of XIST was positively correlated with the PASI score and serum tumor necrosis factor-alpha (TNF-α), interleukin-17A [IL-17A], and IL-22 concentrations in psoriasis patients. XIST silencing suppressed M5-induced keratinocyte proliferation and restrained the discharge of inflammatory cytokines (TNF-α, IL-17A, IL-22) and chemokines (CXCL1, CXCL8, CCL20). XIST can sponge miR-338-5p, and miR-338-5p downregulation abolished the inhibitory effect of XIST silencing on cell proliferation and inflammation. miR-338-5p was highly expressed in the clinical serum samples from psoriasis patients. The target relationship between miR-338-5p and IL-6 was proved. Conclusion: LncRNA XIST is highly expressed in the serum of patients with psoriasis, and was positively correlated with disease severity and inflammation. XIST may regulate keratinocyte proliferation and inflammation via regulating miR-338-5p/IL-6 axis.","PeriodicalId":21748,"journal":{"name":"Skin Pharmacology and Physiology","volume":"35 1","pages":"196 - 205"},"PeriodicalIF":2.7,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44840053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Front & Back Matter 正面和背面事项
IF 2.7 4区 医学 Q2 DERMATOLOGY Pub Date : 2022-03-01 DOI: 10.1159/000523930
J. Fluhr, M. Lane
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引用次数: 0
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Skin Pharmacology and Physiology
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