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[Microfluidics-driven synthetic biology and biomanufacturing: research progress in methods and applications and future perspectives]. [微流体驱动的合成生物学和生物制造:方法、应用和未来展望的研究进展]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250444
Huiling Yuan, Guotian Song, Xianni Qi, Yuanyuan Zhang, Li Chen, Nan Xiang, Qinhong Wang

Synthetic biology and biomanufacturing, two cutting-edge directions in biotechnology, are rapidly developing and regarded as the core driving force of the "third biotechnology revolution". They reshape the paradigm of industrial manufacturing through designing life and making useful products and promote the industrial revolution in healthcare, energy, agriculture, and environmental protection. However, the development of synthetic biology and biomanufacturing, especially in terms of designing life for practical applications, still faces challenges such as insufficient screening capabilities, culture heterogeneity, and limited regulation and control over biological processes. Microfluidics, with its micro-scale fluid manipulation capability, offers new solutions to these challenges through single-cell precision, high-throughput screening, and rapid iteration. This study reviews microfluidics regarding the applications in the design-build-test-learn (DBTL) cycle of synthetic biology and the role in intensifying biomanufacturing processes, including upstream stain and culture improvement and downstream processing integration and optimization. Then, some case studies of microfluidics-driven synthetic biology and biomanufacturing in the fields of chemicals & materials, agrifood, and healthcare were summarized. Finally, the bottlenecks of microfluidics in synthetic biology and biomanufacturing, such as micro-scale amplification and chip materials, were analyzed, and the future directions, including its role as an enabling technology, interdisciplinary integration, and AI-driven intelligent systems, were discussed. This review is expected to serve as a valuable reference for further innovation in synthetic biology and biomanufacturing.

合成生物学和生物制造是生物技术的两个前沿方向,正在迅速发展,被视为“第三次生物技术革命”的核心驱动力。他们通过设计生活和制造有用的产品,重塑工业制造的范式,推动医疗、能源、农业和环保领域的工业革命。然而,合成生物学和生物制造的发展,特别是在设计生命的实际应用方面,仍然面临着诸如筛选能力不足、培养异质性和对生物过程的调控有限等挑战。微流体技术凭借其微尺度流体操作能力,通过单细胞精度、高通量筛选和快速迭代,为这些挑战提供了新的解决方案。本研究综述了微流体在合成生物学设计-构建-测试-学习(DBTL)循环中的应用,以及微流体在强化生物制造过程中的作用,包括上游染色和培养改进以及下游工艺集成和优化。然后,总结了微流体驱动合成生物学和生物制造在化工材料、农业食品和医疗保健等领域的一些案例研究。最后,分析了微流体在合成生物学和生物制造领域的瓶颈,如微尺度放大和芯片材料,并讨论了其未来的发展方向,包括其作为使能技术的作用、跨学科集成和人工智能驱动的智能系统。希望对合成生物学和生物制造的进一步创新提供有价值的参考。
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引用次数: 0
[Progress in the research and application of wheat grain protein]. [小麦籽粒蛋白的研究与应用进展]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250440
Wenjia Zhang, Xin Gao, Lei Guo, Danping Li, Yinying Wu, Shengyuan Lv, Yirui Wang, Xiaoyan Duan, Xiukun Liu, Aifeng Liu, Haosheng Li, Jianjun Liu, Zhendong Zhao, Xinyou Cao

Common wheat (Triticum aestivum L.) is one of the main staple food crops in China. The protein quality of wheat grains directly determines the processing quality and nutritional quality of flour foods. With the growth of the population, the upgrading of the dietary structure, and the transformation of the demand in the food industry, the research on wheat in China has shifted from simply pursuing high yields to a new stage of coordinated improvement of yield, quality, and nutrition. This article systematically reviews the main progress in the research on the quality of wheat grain protein, including the identification of gluten proteins and high-quality subunits, the analysis of the expression regulation of gluten protein genes, the mining of quality-related genes based on multi-omics, the impact of the interaction between proteins and other components on processing characteristics, and the application of biotechnology in the breeding of high-quality wheat. In view of the complex evaluation process and environmental susceptibility of wheat quality traits, as well as the goal of achieving synergistic optimization of nutrition and functionality in protein quality research under the National Whole Grain Action Plan, we examine the challenges and future development prospects of cutting-edge technologies such as marker-assisted selection and gene editing. This review aims to provide theoretical support for the upgrading of the high-quality wheat industry.

普通小麦(Triticum aestivum L.)是中国主要粮食作物之一。小麦籽粒的蛋白质质量直接决定着面粉食品的加工质量和营养品质。随着人口的增长、膳食结构的升级和食品产业需求的转变,中国小麦研究已经从单纯追求高产转向了产量、品质、营养协同提升的新阶段。本文系统综述了小麦籽粒蛋白品质研究的主要进展,包括面筋蛋白和优质亚基的鉴定、面筋蛋白基因的表达调控分析、基于多组学的品质相关基因挖掘、蛋白与其他组分相互作用对加工特性的影响以及生物技术在优质小麦育种中的应用等。鉴于小麦品质性状的复杂评价过程和环境敏感性,以及国家“全谷物行动计划”在蛋白质品质研究中实现营养与功能协同优化的目标,我们探讨了标记辅助选择和基因编辑等前沿技术面临的挑战和未来发展前景。本文旨在为优质小麦产业升级提供理论支持。
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引用次数: 0
[Preparation of polyclonal antibodies against Mhp271 and their application in combating Mycoplasma hyopneumoniae infections]. [Mhp271多克隆抗体的制备及其在肺炎支原体感染中的应用]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250493
Huanjun Zhao, Tong Liu, Qi Wu, Yukun Wei, Jiuqing Xin, Qiao Pan

The objective of this study was to prepare polyclonal antibodies (pAb) against the Mycoplasma hyopneumoniae (Mhp) membrane protein Mhp271 and systematically evaluate their immunological characteristics and potential applications. The mhp271 gene (3 156 bp) was amplified by PCR and cloned into the prokaryotic expression vector pMAL-c5x to construct the recombinant plasmid pMAL-c5x-mhp271. After verification by PCR and DNA sequencing, the construct was transformed into Escherichia coli BL21(DE3) competent cells, and the expression of recombinant Mhp271 (rMhp271) was induced with IPTG. Western blotting revealed a specific band at approximately 160 kDa, confirming successful expression of rMhp271. Purified rMhp271 was emulsified and used to immunize BALB/c mice three times. Serum samples were collected one week after the final immunization, and anti-Mhp271 specific pAb was isolated. To assess the reactivity of the anti-Mhp271 pAb, we used porcine alveolar macrophages (PAMs) to establish a cell model of Mhp infection. After Mhp infection for 12 h, Western blotting and indirect immunofluorescence assay (IFA) were employed to assess protein expression. Western blotting results showed a specific band at approximately 118 kDa in the lysate from Mhp-infected PAMs, while no corresponding band was detected in the uninfected control group. IFA demonstrated distinct green fluorescence signals in infected cells, whereas no fluorescence was observed in the uninfected control group. Furthermore, the potential of anti-Mhp271 pAb to inhibit Mhp infection was evaluated through in vitro blocking assays. Mhp was pre-incubated with either 100-fold diluted anti-Mhp271 serum (experimental group) or negative serum (control group) for 30 min before being inoculated into PAMs for 12 h. TaqMan real-time quantitative PCR indicated a significant reduction in Mhp load in the experimental group compared with the control group, which was further confirmed by the weakened fluorescence in IFA. Overall, the prepared anti-Mhp271 pAb demonstrated good reactogenicity and anti-infective activity, being suitable for immunological detection methods such as Western blotting and IFA.

本研究的目的是制备抗肺炎支原体(Mhp)膜蛋白Mhp271的多克隆抗体(pAb),并对其免疫学特性和应用前景进行系统评价。通过PCR扩增mhp271基因(3 156 bp),将其克隆到原核表达载体pMAL-c5x中,构建重组质粒pMAL-c5x-mhp271。经PCR和DNA测序验证后,将构建物转化到大肠杆菌BL21(DE3)感受态细胞中,用IPTG诱导表达重组Mhp271 (rMhp271)。Western blotting结果显示rMhp271在约160 kDa处有特异条带,证实rMhp271成功表达。将纯化的rMhp271进行乳化,用于三次免疫BALB/c小鼠。末次免疫1周后采集血清样本,分离抗mhp271特异性pAb。为了评估抗mhp271 pAb的反应性,我们利用猪肺泡巨噬细胞(PAMs)建立了Mhp感染的细胞模型。Mhp感染12 h后,采用Western blotting和间接免疫荧光法(IFA)检测蛋白表达。Western blotting结果显示,在mhp感染的pam裂解液中约有118 kDa的特异条带,而在未感染的对照组中未检测到相应的条带。IFA在感染细胞中显示出明显的绿色荧光信号,而在未感染的对照组中未观察到荧光。此外,通过体外阻断实验评估了抗mhp271 pAb抑制Mhp感染的潜力。将Mhp与100倍稀释的抗mhp271血清(实验组)或阴性血清(对照组)预孵育30 min后,接种于PAMs中12 h。TaqMan实时定量PCR结果显示,实验组的Mhp负荷较对照组明显减少,IFA荧光减弱进一步证实了这一点。综上所述,制备的抗mhp271抗体具有良好的反应性和抗感染活性,适合于Western blotting和IFA等免疫检测方法。
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引用次数: 0
[Response mechanisms of crops to drought stress and strategies of molecular breeding for drought resistance]. [作物对干旱胁迫的响应机制及抗旱分子育种策略]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250448
Ying Liu, Yaoxu Wang, Xinzhu Zou, Guang Yang, Sheng Ren, Zhaoshi Xu

Drought stress impacts the growth, development, and yields of crops and is a major factor limiting global agricultural production. This paper primarily reviews the molecular mechanisms underlying crop responses to drought stress and the molecular strategies for breeding drought-resistant varieties. We first elaborate on the perception mechanisms of plants to drought stress and delve into the core signal transduction pathways underpinning drought stress responses, the transcriptional regulation and expression of drought-responsive genes, and the signaling pathways of other plant hormones involved in drought stress responses of crops. Additionally, we summarize the molecular strategies for breeding drought-resistant crops, including the identification of candidate drought resistance genes, molecular marker-assisted breeding, multi-omics-assisted breeding, and advancements in transgenic and gene-editing technologies. Finally, we introduce the current status, research hotspots, and future directions in the research on crop drought stress. This paper aims to provide a comprehensive reference for understanding the mechanisms of crop responses to drought stress and facilitate the breeding of drought-resistant crop varieties.

干旱胁迫影响作物的生长、发育和产量,是限制全球农业生产的主要因素。本文主要综述了作物对干旱胁迫响应的分子机制以及选育抗旱品种的分子策略。本文首先阐述了植物对干旱胁迫的感知机制,深入探讨了干旱胁迫响应的核心信号转导途径、干旱响应基因的转录调控和表达,以及其他参与作物干旱胁迫响应的植物激素的信号转导途径。此外,我们还总结了抗旱作物的分子育种策略,包括候选抗旱基因的鉴定、分子标记辅助育种、多组学辅助育种以及转基因和基因编辑技术的进展。最后,介绍了作物干旱胁迫研究的现状、研究热点及未来发展方向。本文旨在为了解作物对干旱胁迫的响应机制和选育抗旱作物品种提供综合参考。
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引用次数: 0
[Research progress in UbiA-type terpene cyclases and their roles in natural product biosynthesis]. [ubia型萜烯环化酶及其在天然产物生物合成中的作用研究进展]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250430
Menghan Zhang, Qian Wang, Zhichao Xu, Chengwei Liu

UbiA-type terpene cyclases represent a class of non-classical terpenoid synthases recently discovered in fungi and bacteria. These enzymes catalyze the cyclization of linear terpene precursors via a carbocation-mediated mechanism, enabling the construction of complex terpene skeletons. The discovery of these enzymes has significantly expanded the enzymatic toolkit for natural product biosynthesis and broadened the structural diversity of terpenoids. Since the identification of FmaTC in Aspergillus fumigatus in 2013, 31 distinct members of the UbiA family have been characterized. We review the research progress in these enzymes, including their conserved motifs, phylogenetic relationships, biosynthetic gene clusters, and the structural diversity of their products. We highlight representative natural products such as sesquiterpenes, diterpenes, and diheterosesquiterpenes, along with their corresponding cyclases. Additionally, we discuss compounds generated through the synergistic action of these cyclases with modifying enzymes such as cytochrome P450s, shedding light on the potential role of UbiA family members in driving natural product diversity. The discovery of UbiA-type terpene cyclases not only enriches the library of enzymes for the biosynthesis of terpenoids but also gives novel insights into the biosynthesis and application of bioactive natural products.

ubia型萜类环化酶是近年来在真菌和细菌中发现的一类非经典萜类合成酶。这些酶通过碳正离子介导的机制催化线性萜烯前体的环化,从而能够构建复杂的萜烯骨架。这些酶的发现极大地扩展了天然产物生物合成的酶工具箱,拓宽了萜类化合物的结构多样性。自2013年在烟曲霉(Aspergillus fumigatus)中发现FmaTC以来,已经鉴定了31个不同的UbiA家族成员。本文综述了这些酶的研究进展,包括它们的保守基序、系统发育关系、生物合成基因簇及其产物的结构多样性。我们重点介绍了具有代表性的天然产物,如倍半萜烯、二萜烯和二异半萜烯,以及它们相应的环化酶。此外,我们还讨论了通过这些环化酶与细胞色素p450等修饰酶的协同作用产生的化合物,揭示了UbiA家族成员在推动天然产物多样性方面的潜在作用。ubia型萜烯环化酶的发现不仅丰富了萜类生物合成酶库,而且为生物活性天然产物的生物合成和应用提供了新的见解。
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引用次数: 0
[Synthetic microbiome design: applications and challenges from theoretical construction to environmental bioremediation]. 合成微生物组设计:从理论构建到环境生物修复的应用与挑战。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250436
Xinyu Lin, Chaoran Li, Xihui Xu, Jiandong Jiang

As artificially constructed microbial communities with specific functions, synthetic microbiomes have shown great application potential in bioremediation in recent years. Compared with single strains, synthetic microbiomes have significant advantages in mineralizing complex pollutants and simultaneously metabolizing multiple pollutants. The construction of synthetic microbiomes involves various strategies and methods, which contribute to the design of microbial communities with diverse functions and adaptability to different environments. Conventional experiment-based studies on synthetic microbiomes often struggle to systematically elucidate the complex metabolic interactions among microbial community members, which limits the design and application of synthetic microbiomes. Genome-scale metabolic model (GSMM) technology has paved new avenues for the design, construction, and functional optimization of synthetic microbiomes. This review concentrates on the advantages of synthetic microbiomes in bioremediation and the current theories and strategies involved in the design and construction of synthetic microbiomes, and highlights the application potential of GSMMs in the design of synthetic microbiomes and the bioremediation of polluted environments. This paper can provide a theoretical basis and technical support for the research on synthetic microbiomes and their application in environmental bioremediation.

人工合成微生物群落作为人工构建的具有特定功能的微生物群落,近年来在生物修复方面显示出巨大的应用潜力。与单一菌株相比,合成菌群在矿化复杂污染物和同时代谢多种污染物方面具有显著优势。合成微生物群落的构建涉及多种策略和方法,有助于设计出功能多样、适应不同环境的微生物群落。传统的基于实验的合成微生物组研究往往难以系统地阐明微生物群落成员之间复杂的代谢相互作用,这限制了合成微生物组的设计和应用。基因组尺度代谢模型(GSMM)技术为合成微生物组的设计、构建和功能优化开辟了新的途径。本文综述了合成微生物组在生物修复中的优势以及目前合成微生物组设计与构建的理论和策略,重点介绍了GSMMs在合成微生物组设计和污染环境生物修复中的应用潜力。本文可为合成微生物组的研究及其在环境生物修复中的应用提供理论依据和技术支持。
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引用次数: 0
[Applications of protein structure determination in synthetic biology]. [蛋白质结构测定在合成生物学中的应用]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250437
Xiaoxuan Fan, Qin Zhou, Qingyan Ruan, Xiaolong Hao, Guoyin Kai

Synthetic biology aims to reconstruct living systems through engineering principles, requiring molecular mechanism elucidation at the atomic level as its theoretical foundation. Protein crystallographic structural analysis, by revealing three-dimensional conformations, dynamic conformational changes, and molecular interaction networks of proteins, provides indispensable atomic-scale blueprints for the functional design and performance optimization of components in synthetic biology. Recent breakthroughs in cryo-electron microscopy, X-ray crystallography, and artificial intelligence-based prediction methods have significantly advanced the applications of protein structure determination in synthetic biology, including protein engineering, metabolic pathway optimization, biosensor design, and artificial biological system construction. This review summarizes key technological advancements in protein structure determination and discusses their specific applications in synthetic biology, along with future development trends, aiming to provide theoretical references and technical perspectives for related research fields.

合成生物学旨在通过工程原理重建生命系统,需要在原子水平上阐明分子机制作为其理论基础。蛋白质晶体结构分析通过揭示蛋白质的三维构象、动态构象变化和分子相互作用网络,为合成生物学中组件的功能设计和性能优化提供了不可或缺的原子尺度蓝图。低温电子显微镜、x射线晶体学和基于人工智能的预测方法的最新突破,极大地推动了蛋白质结构测定在合成生物学中的应用,包括蛋白质工程、代谢途径优化、生物传感器设计和人工生物系统构建。本文综述了蛋白质结构测定的关键技术进展,并对其在合成生物学中的具体应用及未来发展趋势进行了探讨,旨在为相关研究领域提供理论参考和技术展望。
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引用次数: 0
[Novel concepts emerged in the development of plant virus vectors]. [在植物病毒载体的发展中出现了新的概念]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250691
Yingying Wang, Yuman Zhang, Rongxiang Fang

There are diverse plant viruses, which cause harm to the growth of almost all crops. With the in-depth research on the mechanisms of virus infection and transmission in host plants and the development of biosynthesis technology, more and more genomes of viruses have been unveiled. These research achievements not only serve as a pillar for the establishment and optimization of plant virus expression vectors but also provide a new opportunity for the research and development of unknown functional genes in host plants. In the last decade, rapid development occurred in the construction, optimization, and application of plant virus vectors. The notable progress ranges from the breakthrough research on a well-known ancient tobacco mosaic virus (TMV) vector to exploring the use of complicated negative-sense RNA virus vectors. Particularly, the application of these virus vectors in the production of plant-derived pharmaceutical proteins and the molecular breeding has garnered increasing attention. The establishment and optimization of each virus vector rely on the solid theoretical knowledge about the replication and transcription mechanisms of the viral genome, as well as the molecular interaction mechanisms between viruses and their hosts. In return, the development of plant virus vectors is conducive to revealing the interactions between viruses and their hosts in new aspects. To facilitate the understanding of the research and application of plant virus vectors, this review expounds on the design and engineering strategies of plant virus vectors, the novel functions of viral movement proteins, host immune responses to virus infections, virus-induced gene editing (VIGE), and application of virus vectors in molecular breeding. Finally, the challenges faced by the development and application of virus vectors are discussed. As the understanding of viral gene regulation and the virus-host interaction mechanisms deepens, more plant viruses with small genomes will serve to improve the grain yield and quality and the human health. The development of plant virus vector has facilitated the evolution of 'a pathogen' into 'a practical tool' that promoted innovation in plant biotechnology research.

植物病毒种类繁多,对几乎所有作物的生长都有危害。随着对病毒在寄主植物中感染和传播机制研究的深入和生物合成技术的发展,越来越多的病毒基因组被揭示出来。这些研究成果不仅为植物病毒表达载体的建立和优化提供了支撑,也为寄主植物中未知功能基因的研究和开发提供了新的契机。近十年来,植物病毒载体的构建、优化和应用得到了快速发展。从著名的古代烟草花叶病毒(TMV)载体的突破性研究到探索复杂的负义RNA病毒载体的使用,取得了显著的进展。特别是这些病毒载体在植物源性药用蛋白生产和分子育种中的应用日益受到人们的关注。每一种病毒载体的建立和优化都依赖于扎实的病毒基因组复制和转录机制的理论知识,以及病毒与宿主的分子相互作用机制。反过来,植物病毒载体的开发有助于从新的角度揭示病毒与宿主的相互作用。本文就植物病毒载体的设计与工程策略、病毒运动蛋白的新功能、宿主对病毒感染的免疫反应、病毒诱导基因编辑(VIGE)技术以及病毒载体在分子育种中的应用等方面进行了综述,以期对植物病毒载体的研究与应用有所了解。最后,对病毒载体的发展和应用面临的挑战进行了讨论。随着对病毒基因调控和病毒与宿主相互作用机制认识的加深,更多的小基因组植物病毒将为提高粮食产量和品质以及人类健康服务。植物病毒载体的发展促进了“病原体”向“实用工具”的进化,促进了植物生物技术研究的创新。
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引用次数: 0
[Advances in metabolic engineering of common yeast species]. [常见酵母菌代谢工程研究进展]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250522
Shichang Liu, Jiaoqi Gao, Yongjin Zhou

Yeast cells are the preferred microbial chassis for metabolic engineering and have been engineered into microbial cell factories for producing biofuels, materials, and chemicals. The development of gene editing technologies and synthetic biology tools has significantly accelerated the metabolic engineering in yeast, enabling the design of complex metabolic pathways and comprehensive metabolic rewiring. This review introduces the physiological characteristics of several common yeast species, compares their advantages and disadvantages as chassis cells for biosynthesis, and shows their current applications. We then summarize the latest research progress in metabolic engineering strategies. Finally, the future research directions of yeast metabolic engineering are prospected in light of the latest technological advances. This review will guide the development and engineering applications of next generation yeast chassis cells.

酵母细胞是代谢工程的首选微生物底盘,已被设计成生产生物燃料、材料和化学品的微生物细胞工厂。基因编辑技术和合成生物学工具的发展显著加快了酵母的代谢工程,使复杂代谢途径的设计和全面的代谢重布线成为可能。本文介绍了几种常见酵母的生理特性,比较了它们作为生物合成基质细胞的优缺点,并介绍了它们的应用现状。总结了代谢工程策略的最新研究进展。最后,结合酵母代谢工程的最新技术进展,展望了酵母代谢工程未来的研究方向。对新一代酵母底盘细胞的开发和工程应用具有一定的指导意义。
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引用次数: 0
[Research advances in ferroptosis in the regulation of metabolic dysfunction-associated steatotic liver disease]. [铁下垂对代谢功能障碍相关脂肪变性肝病的调控研究进展]。
Q4 Biochemistry, Genetics and Molecular Biology Pub Date : 2025-11-25 DOI: 10.13345/j.cjb.250674
Jingrui Wang, Ren Sheng, Shunwang Li, Yuhao Zhang, Xinwei Wei, Yanni Xu, Shuyi Si

Ferroptosis is a novel type of programmed cell death discovered in recent years. Inhibiting ferroptosis can reduce lipid peroxidation, decrease liver cell necrosis, and inhibit inflammatory responses. Therefore, targeting key targets in the ferroptosis pathway may become a new strategy for the treatment of metabolic dysfunction-associated steatotic liver disease (MASLD). This article reviews the research advances in the roles, molecular mechanisms, signaling pathways, and drug applications of ferroptosis in MASLD, providing a reference for the development of drugs for MASLD.

铁下垂是近年来发现的一种新型程序性细胞死亡。抑制铁下垂可减少脂质过氧化,减少肝细胞坏死,抑制炎症反应。因此,针对铁下垂途径中的关键靶点可能成为治疗代谢功能障碍相关脂肪变性肝病(MASLD)的新策略。本文就铁下垂在MASLD中的作用、分子机制、信号通路及药物应用等方面的研究进展进行综述,为MASLD药物的开发提供参考。
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引用次数: 0
期刊
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
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