Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-243-264
M. Yanitsky, Alexey A. Utyuganov, Mihail S. Ivanov, L. Frolova
Background. The article discusses the problem of psychological health safety of military university cadets, defined in the study through the concept of neuro-psychic tension. The content of the cadets’ attitude to time as a personal correlate of psychological health is analyzed. Materials and methods. To study the attitude to time, Wasserman’s «Semantic Time Differential» and F. Zimbardo ZTPI techniques are used. To measure the level of neuro-psychic tension, an abbreviated version of the MMPI technique and a method for measuring the level of neuropsychic stability are used. Statistical methods (Mann-Whitney comparative analysis, cluster analysis) were used in the processing. The study was conducted at the Novosibirsk Military Institute of the National Guard Troops of the Russian Federation on a sample of 172 cadets. Results. The study shows statistically significant differences in the characteristics of the time perspective and the semantic content of the attitude to time between cadets with different levels of neuro-psychic tension. Conclusion. It is concluded that there is a nonlinear relationship between the studied characteristics according to the principle of «optimum»: the optimal indicators of the attitude to time correspond to the normal (average) level of neuro-psychic tension.
{"title":"CHARACTERISTICS OF THE RELATIONSHIP BETWEEN THE EXPERIENCE OF TIME AND THE LEVEL OF NEURO-MENTAL STRESS IN MILITARY UNIVERSITY CADETS","authors":"M. Yanitsky, Alexey A. Utyuganov, Mihail S. Ivanov, L. Frolova","doi":"10.12731/2658-6649-2023-15-2-243-264","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-243-264","url":null,"abstract":"Background. The article discusses the problem of psychological health safety of military university cadets, defined in the study through the concept of neuro-psychic tension. The content of the cadets’ attitude to time as a personal correlate of psychological health is analyzed. \u0000Materials and methods. To study the attitude to time, Wasserman’s «Semantic Time Differential» and F. Zimbardo ZTPI techniques are used. To measure the level of neuro-psychic tension, an abbreviated version of the MMPI technique and a method for measuring the level of neuropsychic stability are used. Statistical methods (Mann-Whitney comparative analysis, cluster analysis) were used in the processing. The study was conducted at the Novosibirsk Military Institute of the National Guard Troops of the Russian Federation on a sample of 172 cadets. \u0000Results. The study shows statistically significant differences in the characteristics of the time perspective and the semantic content of the attitude to time between cadets with different levels of neuro-psychic tension. \u0000Conclusion. It is concluded that there is a nonlinear relationship between the studied characteristics according to the principle of «optimum»: the optimal indicators of the attitude to time correspond to the normal (average) level of neuro-psychic tension.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49568813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-303-336
Simbo Diakite, E. Pakina, Abdullah Behzad, M. Zargar, F. Saquee, E. V. Kalabashkina, V. A. Tsymbalova, T. Astarkhanova
Background. Global climate change and excessive nitrogen application has become a significant issue and inevitably threatens sustainable wheat production, not only with direct negative effects on crop growth but also with profound impacts on biology and pest and disease management. Purpose. This review addresses the current challenges, namely the negative effects of climate change and the forms and excess of nitrogen-rich fertilizers on the development of fungal diseases in wheat, as well as management strategies. Materials and methods. To achieve the stated objective of the study, the scientific literature published during the last 20 years on the impacts of climate change and the forms and excesses of nitrogen fertilizers on the development of fungal diseases and on the yield of wheat were reviewed. Results. Thus, in mitigating these challenges, it is necessary to optimize the dose of nitrogen fertilizers, apply nitrogen in the form of nitrate, ammonium sulphate, ammonium nitrate, and coated urea fertilizers, to use silicate fertilizers such as calcium, magnesium, and potassium silicate, and to perform a long rotation of wheat through perennial legumes and leguminous crops, as well as to develop, through genome editing, varieties with high yield potential, resistant to biotic and abiotic stresses, and of good end-use quality, or plant new cereals that have needs for heat and a longer reproductive growth period. Conclusion. To develop an effective agricultural management strategy, future research should be based on the study of the interactions among crops, pests, pathogens and farming system under climate change, taking into account all parameters such as temperature increase and CO2, extreme precipitation, etc. A sufficient number of results must be published to be able to draw meaningful conclusions.
{"title":"IMPACTS OF CLIMATE CHANGE, FORMS, AND EXCESS OF NITROGEN FERTILIZERS ON THE DEVELOPMENT OF WHEAT FUNGAL DISEASES","authors":"Simbo Diakite, E. Pakina, Abdullah Behzad, M. Zargar, F. Saquee, E. V. Kalabashkina, V. A. Tsymbalova, T. Astarkhanova","doi":"10.12731/2658-6649-2023-15-2-303-336","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-303-336","url":null,"abstract":"Background. Global climate change and excessive nitrogen application has become a significant issue and inevitably threatens sustainable wheat production, not only with direct negative effects on crop growth but also with profound impacts on biology and pest and disease management. \u0000Purpose. This review addresses the current challenges, namely the negative effects of climate change and the forms and excess of nitrogen-rich fertilizers on the development of fungal diseases in wheat, as well as management strategies. \u0000Materials and methods. To achieve the stated objective of the study, the scientific literature published during the last 20 years on the impacts of climate change and the forms and excesses of nitrogen fertilizers on the development of fungal diseases and on the yield of wheat were reviewed. \u0000Results. Thus, in mitigating these challenges, it is necessary to optimize the dose of nitrogen fertilizers, apply nitrogen in the form of nitrate, ammonium sulphate, ammonium nitrate, and coated urea fertilizers, to use silicate fertilizers such as calcium, magnesium, and potassium silicate, and to perform a long rotation of wheat through perennial legumes and leguminous crops, as well as to develop, through genome editing, varieties with high yield potential, resistant to biotic and abiotic stresses, and of good end-use quality, or plant new cereals that have needs for heat and a longer reproductive growth period. \u0000Conclusion. To develop an effective agricultural management strategy, future research should be based on the study of the interactions among crops, pests, pathogens and farming system under climate change, taking into account all parameters such as temperature increase and CO2, extreme precipitation, etc. A sufficient number of results must be published to be able to draw meaningful conclusions.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47990477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the influence of fossil shell flour (FSF) supplementation levels on Dohne-Merino wethers’ position on enteric methane output. Twenty-four Dohne-Merino wethers (20.0±1.50 kg B.W.) were randomly assigned for 84 days to either of four dietary treatments: basal or basal diet supplemented with 2%,4% or 6%FSF on a dry matter basis. The enteric methane output was measured using a portable Laser Methane Detector (LMD) machine during feeding, standing, and resting activities. The highest volume of enteric methane was obtained from wethers supplemented with 4%, followed by 6%, 0%, and 2% FSF. Higher enteric methane emission was observed for resting wethers than those feeding and standing (P < 0.05). Including fossil shell flour in Dohne-merino wethers’ diets at 4% and 6% increases enteric methane output (P<0.05). Dohne-merino sheep emit more enteric methane when resting than when feeding or standing idle.
本研究旨在调查化石贝壳粉(FSF)的补充水平对多恩-梅里诺羊肠道甲烷输出位置的影响。24只多恩美利奴羊(20.0±1.50 kg B.W.)被随机分配到四种饮食处理中的任何一种,为期84天:基础或基础饮食,在干物质基础上添加2%、4%或6%的FSF。在喂食、站立和休息活动期间,使用便携式激光甲烷探测器(LMD)测量肠道甲烷输出。添加4%的羊肠道甲烷含量最高,其次是6%、0%和2%的FSF。休息的羊的肠道甲烷排放量高于喂食和站立的羊(P<0.05)。在多恩美利奴羊的日粮中加入4%和6%的化石壳粉可增加肠道甲烷排放(P<0.05),多恩美利奴羊在休息时比喂食或闲置时排放更多的肠道甲烷。
{"title":"EFFECT OF DIETARY SUPPLEMENTATION WITH FOSSIL SHELL FLOUR ON ENTERIC METHANE OUTPUT AND POSITION-DEPENDENT VARIATIONS IN DOHNE-MERINO WETHERS","authors":"Oyebade Ikusika Olusegun, Thando Mpendulo Conference","doi":"10.12731/2658-6649-2023-15-2-163-177","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-163-177","url":null,"abstract":"This study aimed to investigate the influence of fossil shell flour (FSF) supplementation levels on Dohne-Merino wethers’ position on enteric methane output. Twenty-four Dohne-Merino wethers (20.0±1.50 kg B.W.) were randomly assigned for 84 days to either of four dietary treatments: basal or basal diet supplemented with 2%,4% or 6%FSF on a dry matter basis. The enteric methane output was measured using a portable Laser Methane Detector (LMD) machine during feeding, standing, and resting activities. The highest volume of enteric methane was obtained from wethers supplemented with 4%, followed by 6%, 0%, and 2% FSF. Higher enteric methane emission was observed for resting wethers than those feeding and standing (P < 0.05). Including fossil shell flour in Dohne-merino wethers’ diets at 4% and 6% increases enteric methane output (P<0.05). Dohne-merino sheep emit more enteric methane when resting than when feeding or standing idle.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49307133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-24-40
Georgiy V. Pestsov, Anastasia Tretyakova, O. V. Prokudina, Sergey Butenko
Background. The problem of agricultural waste disposal is currently very relevant. In the Russian Federation, the agro-industrial complex annually produces hundreds of millions of tons of waste that must be disposed of and used rationally. Organic waste from agricultural production and farm animal manure can be effectively disposed of using directed microbiological fermentation and larvae of the insect Hermetia illucens (black soldier fly). As a result of the activity of the enzyme system of black lion larvae and specific microflora, agricultural waste turns into zoohumus with a high content of nitrogen, phosphorus, potassium and trace elements, which can be used as an organic fertilizer that improves the quality of the soil, and the biomass of larvae for animal feed and for obtaining protein concentrate and fat. Purpose. To determine the optimal temperature conditions for the cultivation of the insect species Hermetia illucens (black soldier fly) on various types of substrates, to study the effect of the substrate composition on the production of larval biomass, protein concentrate and fat. Materials and Methods. The research work was carried out in the microbiological laboratory of the Center for Technological Excellence “Advanced Chemical and Biotechnology”, laboratories of the Department of Biology and Technologies of Living Systems, Faculty of Natural Sciences of the Tula State Pedagogical University named after L. N. Tolstoy and insectaria LLC “Lvinka”. To study the effect of the feed composition on the growth and development of the larvae of the insect species Hermetia illucens, substrates from various agricultural, food and animal waste were used. To determine the influence of temperature and humidity on the development of larvae, thermostats and insectarium rooms were used. Results. The study of the effect of various solid organic waste on the biomass of live and dried larvae showed that the larvae of the insect H. illucens grew and developed better on food waste consisting of meat and fish waste, as well as plant food waste. Larvae developed worse on plant residues. This is due to the fact that these wastes contain a large amount of cellulose and much less nutrients in their composition, therefore, on a substrate of this composition, the larvae gained biomass worse. The optimal temperature for the cultivation of insect larvae of the species H. illucens is t-22°C. The ratio of protein and fat in the larvae of the insect H. illucens depends on the composition of the feed. In those types of feed where protein predominates, the content of this component in the larvae is high. For example, when using food meat waste, the content of protein concentrate reaches 595 g/kg, fat only 323 g/ kg, and when using vegetable waste containing a large amount of carbohydrates, more fat and less protein are produced. Concisions. As a result of the research work carried out, it was possible to study the influence of environmental factors on the growth a
{"title":"STUDY OF THE INFLUENCE OF ENVIRONMENTAL FACTORS ON THE GROWTH AND DEVELOPMENT OF THE INSECT SPECIES HERMETIA ILLUCENS (BLACK SOLDIER FLY)","authors":"Georgiy V. Pestsov, Anastasia Tretyakova, O. V. Prokudina, Sergey Butenko","doi":"10.12731/2658-6649-2023-15-2-24-40","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-24-40","url":null,"abstract":"Background. The problem of agricultural waste disposal is currently very relevant. In the Russian Federation, the agro-industrial complex annually produces hundreds of millions of tons of waste that must be disposed of and used rationally. Organic waste from agricultural production and farm animal manure can be effectively disposed of using directed microbiological fermentation and larvae of the insect Hermetia illucens (black soldier fly). As a result of the activity of the enzyme system of black lion larvae and specific microflora, agricultural waste turns into zoohumus with a high content of nitrogen, phosphorus, potassium and trace elements, which can be used as an organic fertilizer that improves the quality of the soil, and the biomass of larvae for animal feed and for obtaining protein concentrate and fat. \u0000Purpose. To determine the optimal temperature conditions for the cultivation of the insect species Hermetia illucens (black soldier fly) on various types of substrates, to study the effect of the substrate composition on the production of larval biomass, protein concentrate and fat. \u0000Materials and Methods. The research work was carried out in the microbiological laboratory of the Center for Technological Excellence “Advanced Chemical and Biotechnology”, laboratories of the Department of Biology and Technologies of Living Systems, Faculty of Natural Sciences of the Tula State Pedagogical University named after L. N. Tolstoy and insectaria LLC “Lvinka”. \u0000To study the effect of the feed composition on the growth and development of the larvae of the insect species Hermetia illucens, substrates from various agricultural, food and animal waste were used. To determine the influence of temperature and humidity on the development of larvae, thermostats and insectarium rooms were used. \u0000Results. The study of the effect of various solid organic waste on the biomass of live and dried larvae showed that the larvae of the insect H. illucens grew and developed better on food waste consisting of meat and fish waste, as well as plant food waste. Larvae developed worse on plant residues. This is due to the fact that these wastes contain a large amount of cellulose and much less nutrients in their composition, therefore, on a substrate of this composition, the larvae gained biomass worse. The optimal temperature for the cultivation of insect larvae of the species H. illucens is t-22°C. The ratio of protein and fat in the larvae of the insect H. illucens depends on the composition of the feed. In those types of feed where protein predominates, the content of this component in the larvae is high. For example, when using food meat waste, the content of protein concentrate reaches 595 g/kg, fat only 323 g/ kg, and when using vegetable waste containing a large amount of carbohydrates, more fat and less protein are produced. \u0000Concisions. As a result of the research work carried out, it was possible to study the influence of environmental factors on the growth a","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41699697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-74-91
Georgiy V. Pestsov, O. V. Prokudina, Anastasia Tretyakova, Sergey Butenko
Background. The insect Hermetia illucens (black soldier fly), is widely used for the disposal of food and agricultural waste. This is due to the omnivorous nature of the fly in relation to protein and carbohydrate foods and a fast reproductive cycle. The big problem of industrial cultivation of this insect is the degeneration of populations of the black soldier fly. When bred in captivity, due to the relatively small number of population, the degradation of key parameters, such as viability, fertility, productivity, etc. of H. illucens culture occurs. It is noted that during the first five generations, despite polygamy during crossing, the effect of harmful recessive alleles in a homozygous state manifests itself. Currently, the problem of degeneration of flies associated with inbred depression is solved by mass cultivation of large outbred populations in cages, with periodic reintroduction of producers from natural populations. Purpose. Development of a method for crossing lines of Hermetia illucens flies for breeding, determining patterns of population development, as well as increasing their productivity and adaptive potential. Materials and Methods. In selection work with the lines of the insect species H. illucens, individual crossing was used. Individual selections were made by selecting larvae and pupae by size and body weight. The crosses were carried out according to the scheme: one male and one female were placed in plastic containers while maintaining the required temperature and humidity. Eggs obtained from this pair of flies were placed on a nutrient substrate in a separate container, hatched larvae were cultured in isolation in the same container. During the growth process, the largest larvae and pupae from this population were selected. After the formation of adult individuals (images) of flies, they were examined, their sex was determined and again one male and one female were placed in a separate container. Thus, inbred lines of isofemale (descendants of one female) flies were obtained. Results. We developed the method of individual crossing made it possible to trace the development of populations of flies obtained from one female and one male, to consider the patterns and changes in the population. The tendency of the degeneration of the fly population in inbred crosses was determined, in each subsequent generation there were fewer fertilized eggs and larvae. This is due to the fact that recessive lethal genes become homozygous and manifest their effect. Subsequent selections in each generation of the largest and most active larvae will create a population devoid of most lethal and harmful genes. Conclusions. As a result of the research work carried out, it was possible to develop a method of individual crossing of males and females of the insect Hermetia illucens. It was determined that the time from oviposition onset to the maximum number of larvae occurs on 3-4th day after egg laying. With the crossing of close relatives,
{"title":"DEVELOPMENT OF METHODS OF BREEDING WORK WITH INSECTS OF THE SPECIES HERMETIA ILLUCENS","authors":"Georgiy V. Pestsov, O. V. Prokudina, Anastasia Tretyakova, Sergey Butenko","doi":"10.12731/2658-6649-2023-15-2-74-91","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-74-91","url":null,"abstract":"Background. The insect Hermetia illucens (black soldier fly), is widely used for the disposal of food and agricultural waste. This is due to the omnivorous nature of the fly in relation to protein and carbohydrate foods and a fast reproductive cycle. The big problem of industrial cultivation of this insect is the degeneration of populations of the black soldier fly. When bred in captivity, due to the relatively small number of population, the degradation of key parameters, such as viability, fertility, productivity, etc. of H. illucens culture occurs. It is noted that during the first five generations, despite polygamy during crossing, the effect of harmful recessive alleles in a homozygous state manifests itself. Currently, the problem of degeneration of flies associated with inbred depression is solved by mass cultivation of large outbred populations in cages, with periodic reintroduction of producers from natural populations. \u0000Purpose. Development of a method for crossing lines of Hermetia illucens flies for breeding, determining patterns of population development, as well as increasing their productivity and adaptive potential. \u0000Materials and Methods. In selection work with the lines of the insect species H. illucens, individual crossing was used. Individual selections were made by selecting larvae and pupae by size and body weight. The crosses were carried out according to the scheme: one male and one female were placed in plastic containers while maintaining the required temperature and humidity. Eggs obtained from this pair of flies were placed on a nutrient substrate in a separate container, hatched larvae were cultured in isolation in the same container. During the growth process, the largest larvae and pupae from this population were selected. After the formation of adult individuals (images) of flies, they were examined, their sex was determined and again one male and one female were placed in a separate container. Thus, inbred lines of isofemale (descendants of one female) flies were obtained. \u0000Results. We developed the method of individual crossing made it possible to trace the development of populations of flies obtained from one female and one male, to consider the patterns and changes in the population. The tendency of the degeneration of the fly population in inbred crosses was determined, in each subsequent generation there were fewer fertilized eggs and larvae. This is due to the fact that recessive lethal genes become homozygous and manifest their effect. Subsequent selections in each generation of the largest and most active larvae will create a population devoid of most lethal and harmful genes. \u0000Conclusions. As a result of the research work carried out, it was possible to develop a method of individual crossing of males and females of the insect Hermetia illucens. It was determined that the time from oviposition onset to the maximum number of larvae occurs on 3-4th day after egg laying. With the crossing of close relatives,","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66376246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-139-162
F. M. Khazieva, I. V. Basalaeva, E. A. Konyaeva, A. E. Burova, Tat’yana Е. Samatadze
Background. Currently, it is quite relevant in breeding to create varieties with high yields of raw materials and the content of biologically active compounds. This work aim is to identify promising forms of P. caeruleum L. is for further inclusion in the breeding process based on biomorphological, anatomical, cytogenetic studies and the bioproductivity of colchicine-induced and wild-growing forms in comparison with the Lazur variety. Materials and methods. To obtain polyploids in 2018, the seeds of the P. caeruleum L. the Lazur variety were germinated in Petri dishes on filter paper at room temperature. When the roots reached the length of the seed, they were placed in different concentrations of colchicine solution 0.05 %, 0.1 %, 0.2 % and 0.52 % for 12 and 24 hours, then washed in running water for three hours. Results. The article for the first time presents data on a comparative study of three forms of Polemonium caeruleum L. in terms of morphological characteristics, microscopy of the leaf epidermis and cytogenetic analysis. It was established that the raw materials productivity of the underground part of the plants tetraploid forms in the C1 generation significantly exceeded the diploid and wild forms of plants by 32 %, as well as the yield of seeds by 22 %. In the C2 generation, the yield of raw materials (rhizomes with roots) in tetraploid plants increases in relation to the control by 56 %, seeds by 34 %. Quantitative determination of the amount of triterpene saponins in terms of β-escin in the plant material of three forms of P. caeruleum L. showed an excess of this indicator in the colchicine form by 24 % in the grass and by 36 % in rhizomes with roots, in the wild-growing form - by 15 % in the aerial part and decrease in the underground part by 9 % in relation to the control. It was found that plants of colchicine and wild forms differ in the presence of larger stomata compared to the control. Based on the pattern of DAPI-bandin and the distribution of 45S rDNA and 5S rDNA genes, a generalized species idiogram of blue cyanosis was constructed, taking into account polymorphic variants of DAPI-bandin patterns and indicating the location of the sites of 45S rDNA and 5S rDNA genes. The cytogenetic stability of the resulting tetraploid form was revealed. Conclusion. The article presents comparative studies on morphological characteristics, bioproductivity, microscopy of the leaf epidermis and cytogenetic analysis of the tetraploid form (colchicine) of P. caeruleum L. in comparison with the variety Lazur (diploid form) and the wild form of P. caeruleum L. plants.
{"title":"SCREENING FOR BIOMORPHOLOGICAL AND CYTOGENETIC FEATURES OF POLEMONIUM CAERULEUM L. AFTER COLCHICINE APPLICATION","authors":"F. M. Khazieva, I. V. Basalaeva, E. A. Konyaeva, A. E. Burova, Tat’yana Е. Samatadze","doi":"10.12731/2658-6649-2023-15-2-139-162","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-139-162","url":null,"abstract":"Background. Currently, it is quite relevant in breeding to create varieties with high yields of raw materials and the content of biologically active compounds. \u0000This work aim is to identify promising forms of P. caeruleum L. is for further inclusion in the breeding process based on biomorphological, anatomical, cytogenetic studies and the bioproductivity of colchicine-induced and wild-growing forms in comparison with the Lazur variety. \u0000Materials and methods. To obtain polyploids in 2018, the seeds of the P. caeruleum L. the Lazur variety were germinated in Petri dishes on filter paper at room temperature. When the roots reached the length of the seed, they were placed in different concentrations of colchicine solution 0.05 %, 0.1 %, 0.2 % and 0.52 % for 12 and 24 hours, then washed in running water for three hours. \u0000Results. The article for the first time presents data on a comparative study of three forms of Polemonium caeruleum L. in terms of morphological characteristics, microscopy of the leaf epidermis and cytogenetic analysis. It was established that the raw materials productivity of the underground part of the plants tetraploid forms in the C1 generation significantly exceeded the diploid and wild forms of plants by 32 %, as well as the yield of seeds by 22 %. In the C2 generation, the yield of raw materials (rhizomes with roots) in tetraploid plants increases in relation to the control by 56 %, seeds by 34 %. Quantitative determination of the amount of triterpene saponins in terms of β-escin in the plant material of three forms of P. caeruleum L. showed an excess of this indicator in the colchicine form by 24 % in the grass and by 36 % in rhizomes with roots, in the wild-growing form - by 15 % in the aerial part and decrease in the underground part by 9 % in relation to the control. It was found that plants of colchicine and wild forms differ in the presence of larger stomata compared to the control. Based on the pattern of DAPI-bandin and the distribution of 45S rDNA and 5S rDNA genes, a generalized species idiogram of blue cyanosis was constructed, taking into account polymorphic variants of DAPI-bandin patterns and indicating the location of the sites of 45S rDNA and 5S rDNA genes. The cytogenetic stability of the resulting tetraploid form was revealed. \u0000Conclusion. The article presents comparative studies on morphological characteristics, bioproductivity, microscopy of the leaf epidermis and cytogenetic analysis of the tetraploid form (colchicine) of P. caeruleum L. in comparison with the variety Lazur (diploid form) and the wild form of P. caeruleum L. plants.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46058081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-284-302
Roman A. Yaskevich, Olga L. Moskalenko
Purpose. To study the features of affective disorders in women with comorbid chronic ischemic heart disease with metabolic syndrome.
Materials and methods. The study was performed with the participation of 160 female patients diagnosed with chronic ischemic heart disease. Metabolic syndrome verification was carried out using the clinical recommendations of the All-Russian Scientific Society of Cardiology (2009). Identification of the presence of anxiety-depressive states and assessment of their severity was carried out using The Hospital Anxiety and Depression scale.
Results. The study showed that 50,9% of the surveyed women with a combination of chronic ischemic heart disease and metabolic syndrome had elevated levels of anxiety. Anxiety disorders of a subclinically pronounced nature were found in 31,1% of the surveyed in this group, and clinically pronounced in 19,6%. Elevated levels of depression were detected in 35,7% of the examined patients with metabolic syndrome. Depressive disorders, which were subclinically pronounced, were found in 18,7% of the examined in this group, and clinically pronounced in 17,0%. The most frequent cluster of metabolic syndrome in the studied group of women, both with an increased level of anxiety and with an increased level of depression, was a three-component cluster of metabolic syndrome – 47,4% and 42,5%.
Conclusion. Among patients with chronic ischemic heart disease with a comorbid course with metabolic syndrome, the number of people with clinically pronounced anxiety and depression was generally higher in comparison with people without metabolic syndrome. At the same time, the number of patients with metabolic syndrome with clinically pronounced depression was twice as high – 17,0% and 8.0%. More often in both groups there was a cluster of metabolic syndrome, including a combination of hypertension with abdominal obesity and lipid disorders.
目的。探讨女性慢性缺血性心脏病伴代谢综合征患者情感性障碍的特点。
材料和方法。这项研究是在160名诊断为慢性缺血性心脏病的女性患者的参与下进行的。代谢综合征验证采用全俄科学心脏病学会(2009)的临床建议进行。使用医院焦虑抑郁量表(the Hospital Anxiety and Depression scale)识别焦虑抑郁状态的存在并评估其严重程度。
结果。研究表明,50.9%患有慢性缺血性心脏病和代谢综合征的受访女性焦虑水平升高。亚临床表现的焦虑障碍在31.1%的被调查者中被发现,在19.6%的被调查者中被发现有临床表现。在接受检查的代谢综合征患者中,35.7%的人发现抑郁水平升高。在这组被检查的患者中,有18.7%的人出现了亚临床表现的抑郁症,有17.0%的人出现了临床表现。在研究组中,最常见的代谢综合征群,既有焦虑水平的增加,也有抑郁水平的增加,是代谢综合征的三组分群- 47.4%和42.5%。结论。在伴有代谢综合征的慢性缺血性心脏病患者中,与没有代谢综合征的患者相比,临床表现出焦虑和抑郁的人数普遍较高。与此同时,伴有临床表现抑郁的代谢综合征患者数量为17.0%和8.0%,是前者的两倍。两组患者更常出现代谢综合征,包括高血压合并腹部肥胖和脂质紊乱。
{"title":"AFFECTIVE DISORDERS IN WOMEN WITH ARTERIAL HYPERTENSION IN COMBINATION WITH METABOLIC SYNDROME","authors":"Roman A. Yaskevich, Olga L. Moskalenko","doi":"10.12731/2658-6649-2023-15-2-284-302","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-284-302","url":null,"abstract":"Purpose. To study the features of affective disorders in women with comorbid chronic ischemic heart disease with metabolic syndrome.
 Materials and methods. The study was performed with the participation of 160 female patients diagnosed with chronic ischemic heart disease. Metabolic syndrome verification was carried out using the clinical recommendations of the All-Russian Scientific Society of Cardiology (2009). Identification of the presence of anxiety-depressive states and assessment of their severity was carried out using The Hospital Anxiety and Depression scale.
 Results. The study showed that 50,9% of the surveyed women with a combination of chronic ischemic heart disease and metabolic syndrome had elevated levels of anxiety. Anxiety disorders of a subclinically pronounced nature were found in 31,1% of the surveyed in this group, and clinically pronounced in 19,6%. Elevated levels of depression were detected in 35,7% of the examined patients with metabolic syndrome. Depressive disorders, which were subclinically pronounced, were found in 18,7% of the examined in this group, and clinically pronounced in 17,0%. The most frequent cluster of metabolic syndrome in the studied group of women, both with an increased level of anxiety and with an increased level of depression, was a three-component cluster of metabolic syndrome – 47,4% and 42,5%.
 Conclusion. Among patients with chronic ischemic heart disease with a comorbid course with metabolic syndrome, the number of people with clinically pronounced anxiety and depression was generally higher in comparison with people without metabolic syndrome. At the same time, the number of patients with metabolic syndrome with clinically pronounced depression was twice as high – 17,0% and 8.0%. More often in both groups there was a cluster of metabolic syndrome, including a combination of hypertension with abdominal obesity and lipid disorders.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135758343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-28DOI: 10.12731/2658-6649-2023-15-1-42-58
N. Lidzhieva, Pavel A. Shaglinov, S. V. Ubushaeva, S. Ulanova, Konstantin A. Badmaev
Background. The assessment of the structure of cenopopulations of early spring juveniles can be used during the organization of biological monitoring at the population level. Such information is also applicable in assessing the situation in coastal ecosystems, for forecasting and reducing the risk of their violations. Purpose. Analysis of the viability of Holosteum glutinosum cenopopulations to characterize their stability in plant communities of the “water-land” ecotone system of the coast of the Chograi reservoir. Materials and methods. The studies were carried out in the growing seasons of 2018, 2019 and 2021 in five coenopopulations of H. glutinosum growing in plant communities located in the structural blocks of the ecotone. The vital spectrum of populations was obtained by the method of Yu.A. Zlobin [3, 10, 18], the vital type - using the Q criterion [3], the vitality of populations – based on the analysis of the vital index IVC [3], the degree of prosperity or depression of populations - the IQ index [4]. Results. During the four-year study period, in 5 populations of H. glutinosum, regardless of their localization in the ecotone, the value of the Q index exceeds the frequency of individuals of the lowest class of vitality, therefore they are classified as the “thriving” vital type. The value of the vitality indices for the entire period of the study is greatest in the cenopopulation No. 1, located in the fluctuation block. The first peak of the increase in vitality occurred at coenopopulation No. 1 in the fluctuation, the second – at coenopopulation No. 4 in the marginal ecotone blocks. Conclusion. The coenopopulations of H. glutinosum in the ecotone had a vital type of “thriving”. Analysis of the dynamics of vitality in the coenopopulations of H. glutinosum revealed that the conditions prevailing in the communities of fluctuation and marginal ecotone blocks provide the greatest vitality of populations No. 1 and No. 4.
{"title":"DYNAMICS OF THE VITALITY OF CENOPOPULATIONS OF HOLOSTEUM GLUTINOSUM (M. BIEB.) FISCH. & C.A. MEY. (CARYOPHYLLACEAE) IN THE ECOTONE SYSTEM “WATER-LAND” OF THE COAST OF THE CHOGRAI RESERVOIR","authors":"N. Lidzhieva, Pavel A. Shaglinov, S. V. Ubushaeva, S. Ulanova, Konstantin A. Badmaev","doi":"10.12731/2658-6649-2023-15-1-42-58","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-1-42-58","url":null,"abstract":"Background. The assessment of the structure of cenopopulations of early spring juveniles can be used during the organization of biological monitoring at the population level. Such information is also applicable in assessing the situation in coastal ecosystems, for forecasting and reducing the risk of their violations. \u0000Purpose. Analysis of the viability of Holosteum glutinosum cenopopulations to characterize their stability in plant communities of the “water-land” ecotone system of the coast of the Chograi reservoir. \u0000Materials and methods. The studies were carried out in the growing seasons of 2018, 2019 and 2021 in five coenopopulations of H. glutinosum growing in plant communities located in the structural blocks of the ecotone. \u0000The vital spectrum of populations was obtained by the method of Yu.A. Zlobin [3, 10, 18], the vital type - using the Q criterion [3], the vitality of populations – based on the analysis of the vital index IVC [3], the degree of prosperity or depression of populations - the IQ index [4]. \u0000Results. During the four-year study period, in 5 populations of H. glutinosum, regardless of their localization in the ecotone, the value of the Q index exceeds the frequency of individuals of the lowest class of vitality, therefore they are classified as the “thriving” vital type. \u0000The value of the vitality indices for the entire period of the study is greatest in the cenopopulation No. 1, located in the fluctuation block. The first peak of the increase in vitality occurred at coenopopulation No. 1 in the fluctuation, the second – at coenopopulation No. 4 in the marginal ecotone blocks. \u0000Conclusion. The coenopopulations of H. glutinosum in the ecotone had a vital type of “thriving”. Analysis of the dynamics of vitality in the coenopopulations of H. glutinosum revealed that the conditions prevailing in the communities of fluctuation and marginal ecotone blocks provide the greatest vitality of populations No. 1 and No. 4.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48494388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-28DOI: 10.12731/2658-6649-2023-15-1-308-326
E. Ilyinskikh, Evgenia N. Filatova, A. V. Reshetova
Background. It is difficult to confirm the diagnosis of the mixed infection of the erythema migrans form of Lyme borreliosis and tick-borne encephalitis at the onset of the disease because of delayed seroconversion. Purpose. Assessment of hemogram predictors to develop a model for early diagnosis of the mixed infection of the erythema form of Lyme borreliosis and tick-borne encephalitis. Materials and methods. The study involved 21 patients with the mixed infection of the erythema form of Lyme borreliosis and tick-borne encephalitis (Group 1) and 25 patients with the erythema form of borreliosis monoinfection (Group 2), who were hospitalized no later than the 7th day of the disease. The hemogram parameters, the leukocyte intoxication index and the body resistance index were analyzed. ROC analysis was used. The logistic regression model was developed using STATISTICA 12.0 modules. Results. The Group 1 patients compared with Group 2 demonstrated significant increases in the levels of the leukocyte intoxication index, and neutrophils, but decreases in the levels of the body resistance index, eosinophils, basophils and platelets. The leukocyte intoxication index was a “very good” predictor of the mixed infection, and the body resistance index, the number of eosinophils, basophils, neutrophils and platelets in peripheral blood had a “good” predictive values. The model included the body resistance index, the number of eosinophils and basophils. Conclusion. The developed model had a “very good” predictive value for the mixed infection early diagnosis of the erythema migrans form of Lyme borreliosis and tick-borne encephalitis.
{"title":"HEMOGRAM INDICATORS ASSESSMENT FOR EARLY PREDICTING THE MIXED INFECTION OF THE ERYTHEMA MIGRANS FORM OF LYME BORRELIOSIS AND TICK-BORNE ENCEPHALITIS","authors":"E. Ilyinskikh, Evgenia N. Filatova, A. V. Reshetova","doi":"10.12731/2658-6649-2023-15-1-308-326","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-1-308-326","url":null,"abstract":"Background. It is difficult to confirm the diagnosis of the mixed infection of the erythema migrans form of Lyme borreliosis and tick-borne encephalitis at the onset of the disease because of delayed seroconversion. \u0000Purpose. Assessment of hemogram predictors to develop a model for early diagnosis of the mixed infection of the erythema form of Lyme borreliosis and tick-borne encephalitis. \u0000Materials and methods. The study involved 21 patients with the mixed infection of the erythema form of Lyme borreliosis and tick-borne encephalitis (Group 1) and 25 patients with the erythema form of borreliosis monoinfection (Group 2), who were hospitalized no later than the 7th day of the disease. The hemogram parameters, the leukocyte intoxication index and the body resistance index were analyzed. ROC analysis was used. The logistic regression model was developed using STATISTICA 12.0 modules. \u0000Results. The Group 1 patients compared with Group 2 demonstrated significant increases in the levels of the leukocyte intoxication index, and neutrophils, but decreases in the levels of the body resistance index, eosinophils, basophils and platelets. The leukocyte intoxication index was a “very good” predictor of the mixed infection, and the body resistance index, the number of eosinophils, basophils, neutrophils and platelets in peripheral blood had a “good” predictive values. The model included the body resistance index, the number of eosinophils and basophils. \u0000Conclusion. The developed model had a “very good” predictive value for the mixed infection early diagnosis of the erythema migrans form of Lyme borreliosis and tick-borne encephalitis.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47956673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-28DOI: 10.12731/2658-6649-2023-15-1-253-293
I. Vaslavskaya, A. Poltarykhin, Andrej A. Kolupaev, Viktoria G. Vorobyeva
Objective. This article is devoted to the analysis of the mechanism for the implementation of the national project «Health», as well as the assessment of the effectiveness of its implementation. Materials and methods. Currently, there is no system for timely and publicly available assessment of the effectiveness of the implementation of the national project «Healthcare» both at the national and regional levels. In this regard, it is proposed to evaluate the effectiveness of the national project according to the methodology based on the integral indicator of effectiveness, developed by the experts of the Higher School of Health Management for assessing the health of the region. Results. The concept and role of national projects in the regional economy as part of strategic planning is given. The management system and the mechanism for the implementation of the national project in health care at the regional level are disclosed. Techniques for evaluating the effectiveness of the implementation of national projects have been studied. The current state of the implementation of the national project «Healthcare» in the Altai Territory is characterized. The mechanism for the implementation of the national project «Healthcare» is analyzed. Conclusion. An assessment of the effectiveness of the implementation of the national project showed an insufficient level of implementation of the program in the Altai Territory during 2019-2020, which was facilitated by the COVID-19 pandemic, which significantly worsened the mortality rate among the adult population.
{"title":"INCREASING THE EFFICIENCY OF THE IMPLEMENTATION OF NATIONAL PROJECTS IN THE FIELD OF HEALTHCARE","authors":"I. Vaslavskaya, A. Poltarykhin, Andrej A. Kolupaev, Viktoria G. Vorobyeva","doi":"10.12731/2658-6649-2023-15-1-253-293","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-1-253-293","url":null,"abstract":"Objective. This article is devoted to the analysis of the mechanism for the implementation of the national project «Health», as well as the assessment of the effectiveness of its implementation. \u0000Materials and methods. Currently, there is no system for timely and publicly available assessment of the effectiveness of the implementation of the national project «Healthcare» both at the national and regional levels. In this regard, it is proposed to evaluate the effectiveness of the national project according to the methodology based on the integral indicator of effectiveness, developed by the experts of the Higher School of Health Management for assessing the health of the region. \u0000Results. The concept and role of national projects in the regional economy as part of strategic planning is given. The management system and the mechanism for the implementation of the national project in health care at the regional level are disclosed. Techniques for evaluating the effectiveness of the implementation of national projects have been studied. The current state of the implementation of the national project «Healthcare» in the Altai Territory is characterized. The mechanism for the implementation of the national project «Healthcare» is analyzed. \u0000Conclusion. An assessment of the effectiveness of the implementation of the national project showed an insufficient level of implementation of the program in the Altai Territory during 2019-2020, which was facilitated by the COVID-19 pandemic, which significantly worsened the mortality rate among the adult population.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43043081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}