Pub Date : 2023-06-30DOI: 10.12731/2658-6649-2023-15-3-26-40
A. Deryugina, M. Ivashchenko, V. Metelin, R. Kovylin, P. Ignatiev
Background. The intensification of animal husbandry leads to a violation of the adaptive capabilities of the body, a decrease in productivity and natural resistance of animals. The study of nonspecific factors of protection of the cow's body will allow us to develop ways to increase the immune status and as additional information can be used in breeding work for the selection of highly resistant parent pairs. Purpose. The study of the cellular and humoral link of nonspecific resistance in the development of a stress reaction in cattle after the action of technological stress on the body of cows. Materials and methods. The work was carried out on 20 clinically healthy highly productive black-and-white cows. The stressor for the cows was the regrouping, the change of attendants, the conduct of veterinary and sanitary manipulations. The total number of leukocytes, leukogram, bactericidal and lysozyme activity of the blood serum of cows were studied by standard clinical and laboratory methods and the morphology of leukocytes on a scanning electron microscope before and 3, 14, 30 days after technological stress. Results. On days 3-14 after stress, leukocytosis, neutrophilia, monocytosis and lymphopenia were detected relative to the initial level. When using high-resolution microscopy using a scanning electron microscope, the appearance of NEToses in the blood of cows after technological stress was detected. A decrease in the bactericidal activity of serum on the 3rd day after stress with its subsequent increase was established, on the contrary, an increase in lysozyme on the 3rd day was accompanied by its subsequent decrease by 30 days after the technological stress relative to the initial level. Conclusion. The data obtained indicate that the body's defenses are a dynamic physiological indicator, which must be considered as a general resistance of the cattle body to stressors in order to prevent the disruption of the body's adaptive capabilities.
{"title":"INFLUENCE OF TECHNOLOGICAL STRESS ON NON-SPECIFIC RESISTANCE OF THE ORGANISM OF COWS","authors":"A. Deryugina, M. Ivashchenko, V. Metelin, R. Kovylin, P. Ignatiev","doi":"10.12731/2658-6649-2023-15-3-26-40","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-3-26-40","url":null,"abstract":"Background. The intensification of animal husbandry leads to a violation of the adaptive capabilities of the body, a decrease in productivity and natural resistance of animals. The study of nonspecific factors of protection of the cow's body will allow us to develop ways to increase the immune status and as additional information can be used in breeding work for the selection of highly resistant parent pairs. \u0000Purpose. The study of the cellular and humoral link of nonspecific resistance in the development of a stress reaction in cattle after the action of technological stress on the body of cows. \u0000Materials and methods. The work was carried out on 20 clinically healthy highly productive black-and-white cows. The stressor for the cows was the regrouping, the change of attendants, the conduct of veterinary and sanitary manipulations. The total number of leukocytes, leukogram, bactericidal and lysozyme activity of the blood serum of cows were studied by standard clinical and laboratory methods and the morphology of leukocytes on a scanning electron microscope before and 3, 14, 30 days after technological stress. \u0000Results. On days 3-14 after stress, leukocytosis, neutrophilia, monocytosis and lymphopenia were detected relative to the initial level. When using high-resolution microscopy using a scanning electron microscope, the appearance of NEToses in the blood of cows after technological stress was detected. A decrease in the bactericidal activity of serum on the 3rd day after stress with its subsequent increase was established, on the contrary, an increase in lysozyme on the 3rd day was accompanied by its subsequent decrease by 30 days after the technological stress relative to the initial level. \u0000Conclusion. The data obtained indicate that the body's defenses are a dynamic physiological indicator, which must be considered as a general resistance of the cattle body to stressors in order to prevent the disruption of the body's adaptive capabilities.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42949000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.12731/2658-6649-2023-15-3-458-474
I. Grekov, I. Aksenov, P. Afonin, Dmitry V. Gorshkov
Background. Information technologies of the Russian Federation Customs Authorities have been made part of the state digital economy, providing interaction between foreign trade participants and the services provided by the Customs Authorities officers. Purpose. Detection innovations of customs services and customs control of goods sent by international mail (on the example of medical preparations) Materials and methods. In the study, the authors used materials of special customs statistics reflected on the website of the Federal Customs Service of Russia, statistics of foreign economic activity reflected on the website of Rosstat, data reflected on the Federal State Unitary Enterprise Russian Post. In this work, dialectical, systemic, historical, logical, formal-legal, comparative research methods were used. Results. Processing the flow of information and revealing potential threats while moving IMPI (including medications) shall become completely automated with the help of a monitoring system, applying the machine learning technology. Conclusion. The scope of customs control exercised over the goods forwarded in IMPI shall be , on the one hand, extensive , and on the other -efficient, without placing excessive burden in the form of money and time costs on the persons involved in forwarding IMPI.
{"title":"MODEL OF DIGITAL TRANSFORMATION OF CUSTOMS SERVICES AIMED AT MAKING CUSTOMS CONTROL OF GOODS SENT BY INTERNATIONAL MAIL MORE EFFECTIVE (WITH REFERENCE TO MEDICAL DRUGS)","authors":"I. Grekov, I. Aksenov, P. Afonin, Dmitry V. Gorshkov","doi":"10.12731/2658-6649-2023-15-3-458-474","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-3-458-474","url":null,"abstract":"Background. Information technologies of the Russian Federation Customs Authorities have been made part of the state digital economy, providing interaction between foreign trade participants and the services provided by the Customs Authorities officers. \u0000Purpose. Detection innovations of customs services and customs control of goods sent by international mail (on the example of medical preparations) \u0000Materials and methods. \u0000 In the study, the authors used materials of special customs statistics reflected on the website of the Federal Customs Service of Russia, statistics of foreign economic activity reflected on the website of Rosstat, data reflected on the Federal State Unitary Enterprise Russian Post. \u0000In this work, dialectical, systemic, historical, logical, formal-legal, comparative research methods were used. \u0000Results. Processing the flow of information and revealing potential threats while moving IMPI (including medications) shall become completely automated with the help of a monitoring system, applying the machine learning technology. \u0000Conclusion. The scope of customs control exercised over the goods forwarded in IMPI shall be , on the one hand, extensive , and on the other -efficient, without placing excessive burden in the form of money and time costs on the persons involved in forwarding IMPI.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43893769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.12731/2658-6649-2023-15-3-41-57
E. V. Zamenina, Natalya I. Ivonina, I. Roshchevskaya
Background. The effect of acute hypoxia on the human heart leads to deterioration of the heart function, this is reflected in an increase of the heterogeneity of the depolarization process, which is difficult to reveal when using a traditional ECG. Purpose. To investigate the electrical activity of the heart during the ventricular depolarization on the thorax surface under the exposure of acute normobaric hypoxia using multichannel ECG mapping. Material and methods. The electrical activity of the heart in apparently healthy young men (20±1 years) was studied during ventricular depolarization under exposure of acute normobaric hypoxia using cardioelectrotopography. Unipolar ECGs were recorded from 64 electrodes on the surface of the chest, simultaneously with bipolar ECGs in limb leads and the amplitudes of R and S waves from the surface electrodes localized in positions V1 and V5 of the traditional ECG were evaluated. Results. It was revealed that during hypoxia, in comparison with the initial state, the pattern of distribution and the spatio-temporal dynamics of positive and negative cardiac potentials reflecting the sequence of myocardial activation on the torso surface did not change significantly and were typical for the period of ventricular depolarization of the healthy person. A decrease in the amplitudes of the maximum extrema of cardiac electric potentials, the amplitudes of the SV1 and RV5 waves at hypoxic exposure was revealed: the amplitude of the maximum decreased from 1.88±0.58 mV in the initial state to 1.83±0.55 mV at hypoxia, the minimum – from -2.08±0.76 mV to -1.85±0.73 mV, the SV1 wave from -1.28±0.25 mV to -1.17±0.18 mV, RV5 wave from 1.21±0.25 mV to 1.05±0.29 mV, respectively, while the duration of ventricular depolarization did not change statistically significantly. Conclusion. Under hypoxic exposure, the examined persons showed a significant decrease in the amplitudes of the maximum extrema of the electric field of the heart, a direct correlation between changes in the amplitudes of the positive extremum and the RV5 wave, with practically unchanged duration of ventricular depolarization and dynamics of the distribution of cardioelectric potentials.
{"title":"ELECTRICAL ACTIVITY OF THE HUMAN HEART DURING THE PERIOD OF VENTRICULAR DEPOLARIZATION UNDER ACUTE NORMOBARIC HYPOXIA","authors":"E. V. Zamenina, Natalya I. Ivonina, I. Roshchevskaya","doi":"10.12731/2658-6649-2023-15-3-41-57","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-3-41-57","url":null,"abstract":"Background. The effect of acute hypoxia on the human heart leads to deterioration of the heart function, this is reflected in an increase of the heterogeneity of the depolarization process, which is difficult to reveal when using a traditional ECG. \u0000Purpose. To investigate the electrical activity of the heart during the ventricular depolarization on the thorax surface under the exposure of acute normobaric hypoxia using multichannel ECG mapping. \u0000Material and methods. The electrical activity of the heart in apparently healthy young men (20±1 years) was studied during ventricular depolarization under exposure of acute normobaric hypoxia using cardioelectrotopography. Unipolar ECGs were recorded from 64 electrodes on the surface of the chest, simultaneously with bipolar ECGs in limb leads and the amplitudes of R and S waves from the surface electrodes localized in positions V1 and V5 of the traditional ECG were evaluated. \u0000Results. It was revealed that during hypoxia, in comparison with the initial state, the pattern of distribution and the spatio-temporal dynamics of positive and negative cardiac potentials reflecting the sequence of myocardial activation on the torso surface did not change significantly and were typical for the period of ventricular depolarization of the healthy person. A decrease in the amplitudes of the maximum extrema of cardiac electric potentials, the amplitudes of the SV1 and RV5 waves at hypoxic exposure was revealed: the amplitude of the maximum decreased from 1.88±0.58 mV in the initial state to 1.83±0.55 mV at hypoxia, the minimum – from -2.08±0.76 mV to -1.85±0.73 mV, the SV1 wave from -1.28±0.25 mV to -1.17±0.18 mV, RV5 wave from 1.21±0.25 mV to 1.05±0.29 mV, respectively, while the duration of ventricular depolarization did not change statistically significantly. \u0000Conclusion. Under hypoxic exposure, the examined persons showed a significant decrease in the amplitudes of the maximum extrema of the electric field of the heart, a direct correlation between changes in the amplitudes of the positive extremum and the RV5 wave, with practically unchanged duration of ventricular depolarization and dynamics of the distribution of cardioelectric potentials.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41601198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.12731/2658-6649-2023-15-3-295-312
S. Fedotkina, Aziza Kh. Akhmineyeva, M. G. Karaylanov
The article analyzes the literature sources of the most promising areas of the development of informatization of health care systems in Russia and abroad. The World Health Organization and the International Telecommunication Union have noted, that for healthcare, digital systems are not only vital, but simply inevitable. Today, more than half of the underdeveloped and developing countries use telemedicine technologies, including using in emergency situations. It is actively gaining momentum in development and is being improved with infocommunication technologies. Purpose of the study. Analysis of the most promising directions for the development of healthcare informatization in Russia and abroad, taking into account the strategic importance of combating chronic non-communicable diseases. Material and methods. An analytical study that systematizes international and Russian experience in the application of telemedicine technologies. Results of the discussion. According to WHO forecasts, at present, not only in the Russian Federation, but throughout the world, there is a tendency towards population aging, which by 2050 will be more than 22%. This leads to demand growth for medical services. The development of digital mobile medicine (mHealth) is gaining popularity around the world, due to the shortage of medical personnel, the wide geographical spread of the population, the inaccessibility of medical care and the high cost of treatment Conclusion. The development and application of digital mobile medicine in the healthcare system has being contributed significantly to reducing the burden of non-communicable diseases, both from a medical and economical point of view.
{"title":"BEST PRACTICES FOR THE APPLICATION OF TELEMEDICAL TECHNOLOGIES IN THE RUSSIAN FEDERATION AND ABROAD","authors":"S. Fedotkina, Aziza Kh. Akhmineyeva, M. G. Karaylanov","doi":"10.12731/2658-6649-2023-15-3-295-312","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-3-295-312","url":null,"abstract":"The article analyzes the literature sources of the most promising areas of the development of informatization of health care systems in Russia and abroad. The World Health Organization and the International Telecommunication Union have noted, that for healthcare, digital systems are not only vital, but simply inevitable. Today, more than half of the underdeveloped and developing countries use telemedicine technologies, including using in emergency situations. It is actively gaining momentum in development and is being improved with infocommunication technologies. \u0000Purpose of the study. Analysis of the most promising directions for the development of healthcare informatization in Russia and abroad, taking into account the strategic importance of combating chronic non-communicable diseases. \u0000Material and methods. An analytical study that systematizes international and Russian experience in the application of telemedicine technologies. \u0000Results of the discussion. According to WHO forecasts, at present, not only in the Russian Federation, but throughout the world, there is a tendency towards population aging, which by 2050 will be more than 22%. This leads to demand growth for medical services. The development of digital mobile medicine (mHealth) is gaining popularity around the world, due to the shortage of medical personnel, the wide geographical spread of the population, the inaccessibility of medical care and the high cost of treatment \u0000Conclusion. The development and application of digital mobile medicine in the healthcare system has being contributed significantly to reducing the burden of non-communicable diseases, both from a medical and economical point of view.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43822658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-92-124
A. Y. Guzenko, A. Solonkin, A. Belyaev, E. Seminchenko
Background. The conditions of the Volgograd region of the Russian Federation are a zone of risky agriculture with an arid climate. This factor cannot be controlled by agro technological techniques on a full scale, even if all technological processes of field work are followed. Based on this, the development of new adaptive technologies, in relation to certain soil and climatic territories, can be attributed to one of the tasks of modern agricultural science. Tillage is the most important production operation in agriculture, both in terms of energy intensity and the impact on the yield of all cultivated crops. It plays a role in improving soil fertility and its safety from water and wind erosion. The results of the experiment with scientific justification and experimental confirmation on the use of various tillage options (dump, dump less, shallow) for growing field crops depending on the influence of prevailing climatic conditions in 2018-2020 on the experimental field of the Federal Research Center of Agroecology of the Russian Academy of Sciences, in the dry-steppe zone of chestnut soils are presented. Purpose – is to identify the influence of tillage and emerging climatic conditions on the weeding and yield of winter wheat, to carry out mathematical processing by the STATISTICA Application 10.0.0.0 and Excel statistical processing program for the possibility of subsequent theoretical determination of the dependence of the yield and weeding of winter wheat crops on various tillage options in the emerging climatic conditions. The novelty of the work lies in the fact that for the first time studies have been carried out and data have been obtained on the dependence of the yield and infestation of winter wheat crops on various tillage options in the prevailing climatic conditions in the dry steppe zone of chestnut soils. Subsequently, it is possible to theoretically substantiate the use of one or another tillage under certain climatic conditions to reduce the infestation of winter wheat crops and increase its yield. Materials and methods. Since winter wheat is grown in extreme temperature conditions, tillage plays an important role in ensuring the best water regime for the plant life cycle. The soil of the experimental site is light chestnut, heavy loamy, with a humus content of 1.74% in the arable layer. The average annual precipitation was 339.7 mm. The technology of cultivation of these crops was generally accepted for the research area. A zoned variety of winter soft wheat Kamyshanka 4 was sown with a seeding rate of 4.5 million pcs/ha with a seeder SKP-2.1 (Omichka) with anchor coulters. When examining weeds, frames measuring 50×50 cm were used. The species composition of weeds was distributed by type (monocotyledonous or dicotyledonous) and life cycle (annual or perennial). Grain yield was determined, as well as the components of winter wheat yield: plant height (cm), number of grains per ear (pcs), grain weight per ear (gr), weight of
{"title":"DEPENDENCE OF THE YIELD OF WINTER WHEAT (TRITICUM AESTIVUM L.) ON SOIL AND CLIMATIC CONDITIONS AND VARIOUS TILLAGES IN THE ZONE OF LIGHT CHESTNUT SOILS OF THE VOLGA-DON INTERFLUVE AREA OF THE SOUTHERN FEDERAL DISTRICT OF THE RUSSIAN FEDERATION","authors":"A. Y. Guzenko, A. Solonkin, A. Belyaev, E. Seminchenko","doi":"10.12731/2658-6649-2023-15-2-92-124","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-92-124","url":null,"abstract":"Background. The conditions of the Volgograd region of the Russian Federation are a zone of risky agriculture with an arid climate. This factor cannot be controlled by agro technological techniques on a full scale, even if all technological processes of field work are followed. Based on this, the development of new adaptive technologies, in relation to certain soil and climatic territories, can be attributed to one of the tasks of modern agricultural science. \u0000Tillage is the most important production operation in agriculture, both in terms of energy intensity and the impact on the yield of all cultivated crops. It plays a role in improving soil fertility and its safety from water and wind erosion. \u0000The results of the experiment with scientific justification and experimental confirmation on the use of various tillage options (dump, dump less, shallow) for growing field crops depending on the influence of prevailing climatic conditions in 2018-2020 on the experimental field of the Federal Research Center of Agroecology of the Russian Academy of Sciences, in the dry-steppe zone of chestnut soils are presented. \u0000Purpose – is to identify the influence of tillage and emerging climatic conditions on the weeding and yield of winter wheat, to carry out mathematical processing by the STATISTICA Application 10.0.0.0 and Excel statistical processing program for the possibility of subsequent theoretical determination of the dependence of the yield and weeding of winter wheat crops on various tillage options in the emerging climatic conditions. \u0000The novelty of the work lies in the fact that for the first time studies have been carried out and data have been obtained on the dependence of the yield and infestation of winter wheat crops on various tillage options in the prevailing climatic conditions in the dry steppe zone of chestnut soils. Subsequently, it is possible to theoretically substantiate the use of one or another tillage under certain climatic conditions to reduce the infestation of winter wheat crops and increase its yield. \u0000Materials and methods. Since winter wheat is grown in extreme temperature conditions, tillage plays an important role in ensuring the best water regime for the plant life cycle. \u0000The soil of the experimental site is light chestnut, heavy loamy, with a humus content of 1.74% in the arable layer. The average annual precipitation was 339.7 mm. The technology of cultivation of these crops was generally accepted for the research area. A zoned variety of winter soft wheat Kamyshanka 4 was sown with a seeding rate of 4.5 million pcs/ha with a seeder SKP-2.1 (Omichka) with anchor coulters. When examining weeds, frames measuring 50×50 cm were used. The species composition of weeds was distributed by type (monocotyledonous or dicotyledonous) and life cycle (annual or perennial). Grain yield was determined, as well as the components of winter wheat yield: plant height (cm), number of grains per ear (pcs), grain weight per ear (gr), weight of","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49637158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-178-194
S. Fedotkina, E. Khugaeva
At the state level, measures have been carried out in different years to form a sustainable understanding of the importance of health as a personal value by the population. This is due to the global strategic interests of the Russian Federation in preserving and strengthening the health of the population. In our study, the awareness of working-age patients of primary health care about the risk factors for the development of cardiovascular diseases and their frequency of occurrence was analyzed, taking into account the standardization of statistical indicators for 5 years (2014-2018) in the office of prevention of hypertension. Materials and methods. The study was conducted on the basis of urban clinics of the Krasnoselsky district of St. Petersburg in two stages. At the first stage, a sociological survey was conducted using a specially designed questionnaire. At the second stage, an analysis of 807 cases of patients aged 18 to 65 years in the office of prevention of arterial hypertension was carried out. As a result of the sociological survey, respondents among both men and women are well informed about the influence of a number of behavioral risk factors on the occurrence and development of cardiovascular diseases. Conclusion. There is a high awareness of risk factors for the development of non-communicable diseases, including cardiovascular diseases, in patients of working age, which is a positive result of the implementation of a population and high-risk strategy. But there are significant reserves for improving behavior for those who are ready to change it in favor of choosing a healthy lifestyle. Within the framework of primary health care, medical examinations and preventive medical examinations are aimed at increasing the awareness of the population about risk factors for the development of non-communicable, including cardiovascular diseases through medical consultations. The work of Health Centers, prevention offices, Health Schools should be aimed at low-informed patients, examine and determine the functional reserves of their body with the possibility of subsequent correction of the identified disorders. In our work, low awareness was noted in 13% of respondents. Low awareness of patients creates a high risk of unfavorable outcomes of diseases. If the patient understands the essence of his illness and the importance of following the doctor’s recommendations, then the situation may change for the better.
{"title":"AWARENESS OF THE POPULATION ABOUT CARDIOVASCULAR FACTORS OF THE DEVELOPMENT OF DISEASES AS A RESULT OF THE APPLICATION OF A POPULATION STRATEGY","authors":"S. Fedotkina, E. Khugaeva","doi":"10.12731/2658-6649-2023-15-2-178-194","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-178-194","url":null,"abstract":"At the state level, measures have been carried out in different years to form a sustainable understanding of the importance of health as a personal value by the population. This is due to the global strategic interests of the Russian Federation in preserving and strengthening the health of the population. \u0000In our study, the awareness of working-age patients of primary health care about the risk factors for the development of cardiovascular diseases and their frequency of occurrence was analyzed, taking into account the standardization of statistical indicators for 5 years (2014-2018) in the office of prevention of hypertension. \u0000Materials and methods. The study was conducted on the basis of urban clinics of the Krasnoselsky district of St. Petersburg in two stages. At the first stage, a sociological survey was conducted using a specially designed questionnaire. At the second stage, an analysis of 807 cases of patients aged 18 to 65 years in the office of prevention of arterial hypertension was carried out. \u0000As a result of the sociological survey, respondents among both men and women are well informed about the influence of a number of behavioral risk factors on the occurrence and development of cardiovascular diseases. \u0000Conclusion. There is a high awareness of risk factors for the development of non-communicable diseases, including cardiovascular diseases, in patients of working age, which is a positive result of the implementation of a population and high-risk strategy. \u0000But there are significant reserves for improving behavior for those who are ready to change it in favor of choosing a healthy lifestyle. \u0000Within the framework of primary health care, medical examinations and preventive medical examinations are aimed at increasing the awareness of the population about risk factors for the development of non-communicable, including cardiovascular diseases through medical consultations. The work of Health Centers, prevention offices, Health Schools should be aimed at low-informed patients, examine and determine the functional reserves of their body with the possibility of subsequent correction of the identified disorders. \u0000In our work, low awareness was noted in 13% of respondents. Low awareness of patients creates a high risk of unfavorable outcomes of diseases. If the patient understands the essence of his illness and the importance of following the doctor’s recommendations, then the situation may change for the better.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43303053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-11-23
D. Dashko, I. Silkin
The development of new technology methods for cryopreservation of animal cells has contributed to the introduction of stem cell banks for clinical use, including transplantation and regenerative veterinary medicine, and their further use to avoid problems of donors’ shortage. The research aims to determine the possibility of using the bone marrow of a domesticated yak as a source of stem cells after slaughter. Moreover, we should determine the suitability of post-mortem biological material for obtaining stem cells based on the index of proliferation and viability of cultured cells. Therefore, we used the following materials and methods: bone marrow, obtained from the femur of a domesticated yak in the post-slaughter period. Bone marrow samples were taken in compliance with the rules of asepsis in a sterile test tube. Thus, we added 0.25% trypsin solution to the biomass (the ratio of bone marrow to the solution is 10:1) and placed it in a refrigerator (t +40 °C) for 24 hours for enzymatic disaggregation. Furthermore, we carried out the culturing of the obtained cells in a CO2 incubator according to the standard method with passivation after the formation of a monolayer by 90%–100%. When culturing a suspension of cells obtained from post-slaughter bone marrow material, we noted the appearance of cell colonies six days after sowing. Periodic passivation of stem cells contributed to an increase in the biomass of actively proliferating cells. In addition, we found that stem cells isolated from post-slaughter bone marrow material of domesticated yak have significant proliferative potential, as evidenced by proliferation indices in the range from the first to the third passages and high cell viability. Thus, one can use the obtained post-mortem material in the form of the bone marrow of a domesticated yak as an additional source of stem cells. This post-mortem biological material is suitable for the isolation of stem cells 72 hours after the slaughter of an animal, which opens up the possibility of its transportation over long distances. The scientific novelty of the research is in the context of the international Convention on Biological Diversity, which means the variability of living organisms from all sources, including ecosystems and ecological complexes they are a part of. Within the strategy and action plan for the conservation of biodiversity of the Russian Federation, the ecosystem service of livestock production is significant for preserving the traditional way of life of the indigenous peoples of Siberia and the Russian Far East (it has regional and local significance). This global problem can be solved by (1) developing cell technologies for species not yet involved in it and (2) improving existing ones.
{"title":"RELEVANCE OF THE USE OF POSTMORTEM BIOMATERIAL OF DOMESTICATED YAK (BOS GRUNNIENS) TO OBTAIN STEM CELLS FROM BONE MARROW","authors":"D. Dashko, I. Silkin","doi":"10.12731/2658-6649-2023-15-2-11-23","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-11-23","url":null,"abstract":"The development of new technology methods for cryopreservation of animal cells has contributed to the introduction of stem cell banks for clinical use, including transplantation and regenerative veterinary medicine, and their further use to avoid problems of donors’ shortage. The research aims to determine the possibility of using the bone marrow of a domesticated yak as a source of stem cells after slaughter. Moreover, we should determine the suitability of post-mortem biological material for obtaining stem cells based on the index of proliferation and viability of cultured cells. Therefore, we used the following materials and methods: bone marrow, obtained from the femur of a domesticated yak in the post-slaughter period. Bone marrow samples were taken in compliance with the rules of asepsis in a sterile test tube. Thus, we added 0.25% trypsin solution to the biomass (the ratio of bone marrow to the solution is 10:1) and placed it in a refrigerator (t +40 °C) for 24 hours for enzymatic disaggregation. Furthermore, we carried out the culturing of the obtained cells in a CO2 incubator according to the standard method with passivation after the formation of a monolayer by 90%–100%. When culturing a suspension of cells obtained from post-slaughter bone marrow material, we noted the appearance of cell colonies six days after sowing. Periodic passivation of stem cells contributed to an increase in the biomass of actively proliferating cells. In addition, we found that stem cells isolated from post-slaughter bone marrow material of domesticated yak have significant proliferative potential, as evidenced by proliferation indices in the range from the first to the third passages and high cell viability. Thus, one can use the obtained post-mortem material in the form of the bone marrow of a domesticated yak as an additional source of stem cells. This post-mortem biological material is suitable for the isolation of stem cells 72 hours after the slaughter of an animal, which opens up the possibility of its transportation over long distances. \u0000The scientific novelty of the research is in the context of the international Convention on Biological Diversity, which means the variability of living organisms from all sources, including ecosystems and ecological complexes they are a part of. Within the strategy and action plan for the conservation of biodiversity of the Russian Federation, the ecosystem service of livestock production is significant for preserving the traditional way of life of the indigenous peoples of Siberia and the Russian Far East (it has regional and local significance). This global problem can be solved by (1) developing cell technologies for species not yet involved in it and (2) improving existing ones.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47916600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-454-469
E. M. Yatsenko, D. S. Baranovsky, M. Pronkevich, E. Isaeva, A. Smirnova, Vasiliy N. Petrov, S. Ivanov, A. Kaprin
In recent years, major attention has been devoted to immunotherapy in oncology. Immunotherapy is a promising treatment approach targeted to inhibit tumor growth via stimulating the body’s antitumor immune response. Purpose. The study was aimed to investigate the relationship between melanoma growth and metastasis in mice with the various schemes of Galavit treatment. Materials and methods. We used B16-F10 melanoma in F1-mouse as an experimental model. We divided animals in 3 groups: control (no therapy after tumor transplantation); immediate Galavit therapy at a dose of 5 mg/mouse after tumor transplantation; postponed Galavit therapy a week after tumor transplantation at doses of 5mg/mouse. We evaluated the rate of tumor node growth, 50-day survival rate and metastatic development after the 50th day follow-up period. Results. We revealed 80% survival rate for 50-day observation period in the control group. Interestingly, the 50-day survival rate was 60% for the animals treated with Galavit immediately after tumor inoculation. In other group with postponed Galavit therapy, survival rate was 100 %. A similar pattern was observed for tumor growth rates. Conclusion. We found that therapy with Galavit a week after tumor inoculation significantly increased the 50-day survival rate, suppressed tumor growth and the development of metastases in comparison with the control group and the group with immediate Galavit administration.
{"title":"EFFECTS OF THE IMMUNOMODULATOR ‘GALAVIT’ ON THE DEVELOPMENT OF B16 MELANOMA IN MICE","authors":"E. M. Yatsenko, D. S. Baranovsky, M. Pronkevich, E. Isaeva, A. Smirnova, Vasiliy N. Petrov, S. Ivanov, A. Kaprin","doi":"10.12731/2658-6649-2023-15-2-454-469","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-454-469","url":null,"abstract":"In recent years, major attention has been devoted to immunotherapy in oncology. Immunotherapy is a promising treatment approach targeted to inhibit tumor growth via stimulating the body’s antitumor immune response. \u0000Purpose. The study was aimed to investigate the relationship between melanoma growth and metastasis in mice with the various schemes of Galavit treatment. \u0000Materials and methods. We used B16-F10 melanoma in F1-mouse as an experimental model. We divided animals in 3 groups: control (no therapy after tumor transplantation); immediate Galavit therapy at a dose of 5 mg/mouse after tumor transplantation; postponed Galavit therapy a week after tumor transplantation at doses of 5mg/mouse. We evaluated the rate of tumor node growth, 50-day survival rate and metastatic development after the 50th day follow-up period. \u0000Results. We revealed 80% survival rate for 50-day observation period in the control group. Interestingly, the 50-day survival rate was 60% for the animals treated with Galavit immediately after tumor inoculation. In other group with postponed Galavit therapy, survival rate was 100 %. A similar pattern was observed for tumor growth rates. \u0000Conclusion. We found that therapy with Galavit a week after tumor inoculation significantly increased the 50-day survival rate, suppressed tumor growth and the development of metastases in comparison with the control group and the group with immediate Galavit administration.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45138374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-363-391
V. Bakharev, G. Y. Mityashin, E. V. Stelmashonok, Vitaly L. Stelmashonok, Grigory G. Chargasiya
This paper examines innovations in the field of ensuring food security for those in need. It is noted that in the modern world, in addition to traditional food supply models (that is, those that set themselves the only goal – to help those in need) innovative methods are emerging (which pursue several goals, one of which is to help those in need). In more detail, this paper discusses innovative methods, to which the authors refer the possibility of creating a public retail network with fixed prices, conducting a markdown procedure, getting food in commercial enterprises for free, obtaining a comprehensive restaurant service at a significantly lower price and self-organization of people in a digital environment. The paper presents a detailed analysis of each of the above methods. The main conclusion of the study is the need to segment the needy depending on their specific needs. The authors propose to distinguish the following groups of people in need: those in need of basic food, those in need of additional food, those in need of socially acceptable practices for obtaining food, the well-off. For each of the selected groups, innovative methods of providing food are proposed, depending on the specifics of the needs of the people in each group. It is noted that considering individual needs while ensuring food security leads to an increase in the quality of life of the population.
{"title":"TRENDS OF EVOLUTION OF FOOD SECURITY: DIGITAL TRANSFORMATION, SOCIAL ENTREPRENEURSHIP AND HUMAN DIGNITY","authors":"V. Bakharev, G. Y. Mityashin, E. V. Stelmashonok, Vitaly L. Stelmashonok, Grigory G. Chargasiya","doi":"10.12731/2658-6649-2023-15-2-363-391","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-363-391","url":null,"abstract":"This paper examines innovations in the field of ensuring food security for those in need. It is noted that in the modern world, in addition to traditional food supply models (that is, those that set themselves the only goal – to help those in need) innovative methods are emerging (which pursue several goals, one of which is to help those in need). In more detail, this paper discusses innovative methods, to which the authors refer the possibility of creating a public retail network with fixed prices, conducting a markdown procedure, getting food in commercial enterprises for free, obtaining a comprehensive restaurant service at a significantly lower price and self-organization of people in a digital environment. The paper presents a detailed analysis of each of the above methods. The main conclusion of the study is the need to segment the needy depending on their specific needs. The authors propose to distinguish the following groups of people in need: those in need of basic food, those in need of additional food, those in need of socially acceptable practices for obtaining food, the well-off. For each of the selected groups, innovative methods of providing food are proposed, depending on the specifics of the needs of the people in each group. It is noted that considering individual needs while ensuring food security leads to an increase in the quality of life of the population.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44374162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-30DOI: 10.12731/2658-6649-2023-15-2-435-453
E. Zinkevich, M. Korgozha, Natalya A. Yatsevich
Background. In the modern educational conditions of Russian reality, the pedagogical community of medical universities at the stage of postgraduate education pays great attention to the readiness of doctors for professional activities, which determines the conduct of research involving the study of the relationship between meaning-life orientations and educational and professional motivation of residents with different levels of professional experience. In the 2021-2022 academic year, a study illustrating the stated problems was conducted at the St. Petersburg State Pediatric Medical University at the Faculty of Clinical Psychology. Purpose. To study the relationship between meaningful life orientations and educational and professional motivation of residents with different levels of professional experience. Materials and methods. The present study involved 65 residents of 2 years of training in various clinical specialties, their average age is 25.5 years. The entire sample of study participants was divided into two subgroups according to the parameter of involvement in professional activities. This parameter was considered necessary for studying the relationship between meaning-life orientation and educational and professional motivation of residents. Of the total number of respondents, 69% combined residency training with work in their specialty, 31% mastered the specialty at the stage of postgraduate education. Permission was obtained from the Ethics Committee of the St. Petersburg State Pediatric Medical University to organize the study. Respondents agreed to participate in the study. To evaluate the results, descriptive statistical methods, a comparative analysis using the U-Mann-Whitney criterion for explanatory variables and the Wilcoxon T-test for dependent variables, a correlation analysis of the relationships of indicators using the Spearman criterion, calculated using the STATISTICA 12.0 application package, were used. Outcomes. The study allowed to form an idea of the relationship between meaning-life orientations and educational and professional motivation of residents with different levels of professional experience. Conclusions. The results of the study will serve as the basis for rethinking the approaches to the organization of the educational process of students at the stage of residency, the search for ways of education and training that ensured the development of their educational and professional motivation and affect the formation of a system of meaning-life orientations.
{"title":"THE RELATIONSHIP OF MEANING-LIFE ORIENTATIONS AND EDUCATIONAL AND PROFESSIONAL MOTIVATION OF RESIDENTS WITH DIFFERENT LEVELS OF PROFESSIONAL EXPERIENCE","authors":"E. Zinkevich, M. Korgozha, Natalya A. Yatsevich","doi":"10.12731/2658-6649-2023-15-2-435-453","DOIUrl":"https://doi.org/10.12731/2658-6649-2023-15-2-435-453","url":null,"abstract":"Background. In the modern educational conditions of Russian reality, the pedagogical community of medical universities at the stage of postgraduate education pays great attention to the readiness of doctors for professional activities, which determines the conduct of research involving the study of the relationship between meaning-life orientations and educational and professional motivation of residents with different levels of professional experience. In the 2021-2022 academic year, a study illustrating the stated problems was conducted at the St. Petersburg State Pediatric Medical University at the Faculty of Clinical Psychology. \u0000Purpose. To study the relationship between meaningful life orientations and educational and professional motivation of residents with different levels of professional experience. \u0000Materials and methods. The present study involved 65 residents of 2 years of training in various clinical specialties, their average age is 25.5 years. The entire sample of study participants was divided into two subgroups according to the parameter of involvement in professional activities. This parameter was considered necessary for studying the relationship between meaning-life orientation and educational and professional motivation of residents. \u0000Of the total number of respondents, 69% combined residency training with work in their specialty, 31% mastered the specialty at the stage of postgraduate education. Permission was obtained from the Ethics Committee of the St. Petersburg State Pediatric Medical University to organize the study. Respondents agreed to participate in the study. To evaluate the results, descriptive statistical methods, a comparative analysis using the U-Mann-Whitney criterion for explanatory variables and the Wilcoxon T-test for dependent variables, a correlation analysis of the relationships of indicators using the Spearman criterion, calculated using the STATISTICA 12.0 application package, were used. \u0000Outcomes. The study allowed to form an idea of the relationship between meaning-life orientations and educational and professional motivation of residents with different levels of professional experience. \u0000Conclusions. The results of the study will serve as the basis for rethinking the approaches to the organization of the educational process of students at the stage of residency, the search for ways of education and training that ensured the development of their educational and professional motivation and affect the formation of a system of meaning-life orientations.","PeriodicalId":21854,"journal":{"name":"Siberian Journal of Life Sciences and Agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45880401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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