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Bacterial Nanocellulose‐Based Composite Biocatalysts for Starch‐to‐Bioethanol Valorization under Simultaneous Saccharification and Fermentation 细菌纳米纤维素复合生物催化剂在糖化和发酵过程中淀粉转化为生物乙醇的研究
Pub Date : 2023-11-03 DOI: 10.1002/star.202300044
Athanasios Drosos, Agapi Dima, Panagiotis Kandylis, Theano Petsi, Poonam S. Nigam, Athanasios A. Koutinas, Maria Kanellaki
Abstract Composite biocatalysts (CB) consisting of amylases and Saccharomyces cerevisiae immobilized separately on bacterial nanocellulose (BNC) are used for the process of simultaneous saccharification and fermentation (SSF) of starch (5%, w/v) for bioethanol production. Parameters such as: i) addition of phosphates and/or divalent metal‐ions salts during the co‐immobilization process of the amylases, ii) required co‐immobilization time, iii) fermentation temperature and initial pH of starch, and iv) CB as single or double freeze‐dried are studied. The utilization of double freeze‐dried CB exhibits fermentation efficiency 89.9% and ethanol yield 0.51 g ethanol g −1 starch while the single freeze‐dried CB 81.1% and 0.46 g ethanol g −1 starch, respectively. In the case of double freeze‐dried CB, the fermentation efficiency decreases by only 27.1% in two‐recycling batches, while in the single freeze‐dried one decreases by 51.3%. The application of double freeze‐dried CB can be used for: i) the eco‐friendly biosynthesis of value‐added bioproducts; ii) the promising option for fuel‐grade bioethanol through starchy wastes or foodstuff starchy residues treatment, and iii) the implementation of industrialization. Finally, to simulate an industrial process of one‐step SSF of starch by applying a CB model, a technoeconomic analysis is evaluated, where using BNCs makes the bioprocess cost‐effective and environmentally favorable, simultaneously.
摘要将淀粉酶和酿酒酵母分别固定化在细菌纳米纤维素(BNC)上的复合生物催化剂(CB)用于淀粉(5%,w/v)同时糖化发酵(SSF)生产生物乙醇。研究了以下参数:1)在淀粉酶共固定过程中添加磷酸盐和/或二价金属离子盐,2)所需共固定时间,3)淀粉的发酵温度和初始pH值,4)单次或双次冷冻干燥的CB。两次冷冻干燥的黑炭黑发酵效率为89.9%,乙醇产率为0.51 g乙醇g - 1淀粉,而单次冷冻干燥的黑炭黑发酵效率为81.1%,乙醇产率为0.46 g乙醇g - 1淀粉。在两次冷冻干燥的情况下,两次循环的发酵效率仅下降27.1%,而在一次冷冻干燥的情况下,发酵效率下降51.3%。双冷冻干燥CB的应用可以用于:i)生态友好型生物合成的增值生物产品;Ii)通过淀粉废物或食品淀粉残渣处理获得燃料级生物乙醇的有希望的选择,以及iii)工业化的实施。最后,通过应用CB模型模拟淀粉一步SSF的工业过程,对技术经济分析进行了评估,其中使用bnc使生物过程同时具有成本效益和环境效益。
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引用次数: 0
Carbohydrate Profiling of Different Accessions of Three Underutilized Legumes: A Potential Source of Valuable Nutraceuticals and Pharmaceuticals 三种未充分利用的豆科植物的碳水化合物分析:一种有价值的营养品和药品的潜在来源
Pub Date : 2023-11-03 DOI: 10.1002/star.202300184
Queeneth A. Oguuniyi, Omonike O. Ogbole, Olufunke D. Akin‐Ajani, Tolulope O. Ajala, Qingting Liu, Ardha Apriyanto, Oluwatoyin A. Odeku, Joerg Fettke, Olaniyi Oyatomi, Michael Abberton
Abstract Carbohydrates are significant components of legumes, and their profiling can provide information about their nutritional value and potential health benefits. This study explores the proximate composition and carbohydrate profiles of accessions of three underutilized legumes, namely, Vigna radiata , Vigna mungo , and Macrotyloma uniflorum . The total starch and soluble sugar are determined. Starch granule morphology and diameter are determined using scanning electron microscopy and chain length distribution of amylopectin is analyzed using Capillary Electrophoresis. All accessions of the legumes vary both in their proximate composition and carbohydrate components. Accessions of M. uniflorum (PI‐658594‐01‐SD) have the highest carbohydrate component (7.6%) while V. radiata (TVR‐42) has the least (4.2%). Macrotyloma uniflorum accensions (PI‐658594‐01‐SD, and PI‐180437‐01‐SD) have the highest starch and sucrose contents, while V. mungo accensions (TVM‐13, and TVM‐11) have the lowest. The glucose and fructose contents are lower than sucrose in all the accessions of the legumes. Macrotyloma uniflorum accensions exhibit larger granules while accessions of Vigna species have the smallest granules. All species accessions exhibit similar amylopectin chain length distribution profiles although accessions of V. mungo slightly differ in their proportion of long and short glucan chains. The three species of underutilized legumes exhibit unique characteristics which make them suitable for consumption and may be exploited as a source of nutraceuticals and pharmaceuticals.
碳水化合物是豆类的重要组成部分,它们的分析可以提供有关其营养价值和潜在健康益处的信息。本研究探讨了三种未充分利用的豆科植物,即辐射豆科植物、芒豆科植物和独花豆科植物的近似组成和碳水化合物分布。测定总淀粉和可溶性糖。用扫描电镜测定了淀粉颗粒的形态和直径,用毛细管电泳分析了支链淀粉的链长分布。所有的豆科植物在其近似组成和碳水化合物成分上都是不同的。单株M. uniflorum (PI‐658594‐01‐SD)的碳水化合物含量最高(7.6%),而V. radiata (TVR‐42)的碳水化合物含量最低(4.2%)。单花大叶瘤株系(PI‐658594‐01‐SD和PI‐180437‐01‐SD)的淀粉和蔗糖含量最高,而芒果蔓株系(TVM‐13和TVM‐11)的淀粉和蔗糖含量最低。所有豆科植物的葡萄糖和果糖含量都低于蔗糖。单花大叶草的颗粒较大,而薇纳草的颗粒最小。所有的物种都表现出相似的支链淀粉链长分布曲线,尽管芒戈葡萄品种的长链和短链比例略有不同。这三种未被充分利用的豆科植物表现出独特的特征,使它们适合消费,并可作为营养品和药品的来源加以开发。
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引用次数: 0
Treatment of Starch Wastewater by α‐Amylase Immobilized on Silica Infused Magnetite Nanoparticles for Maltose Syrup Preparation 二氧化硅注入磁性纳米颗粒固定化α‐淀粉酶处理淀粉废水制备麦芽糖糖浆
Pub Date : 2023-11-02 DOI: 10.1002/star.202300102
Harsh Patel, Rucha P. Desai, Darshan Patel, Bhavtosh A. Kikani
Abstract Casein decorated silicainfused magnetite (Fe 3 O 4 ‐SiO 2 ) nanoparticles are employed to immobilize commercial α‐amylase, where glutaraldehyde serves as a cross linker. The optimal concentration of variables, such as casein (1.4%w/v), Fe 3 O 4 ‐SiO 2 (106 µl), glutaraldehyde (55µl) and amylase (1 mg/ml) are defined by a Box Behnken design. The binding of casein, glutaraldehyde and enzyme over the nanoparticles are further confirmed structurally by fourier transform infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA). The loading capacity is 37 µg amylase over 1 µg of silica infused magnetite nanoparticles. The optimum pH for the catalysis of soluble and immobilized amylases is the same, i.e. pH 7. However, the pH range for catalysis is improved upon immobilization. The temperature optimum of soluble and immobilized amylases are 40 and 70 °C, respectively. The amylase stability is improved upon immobilization, as shown by enhanced half‐life and reduced deactivation rate constant. The immobilized amylase is used for 17 consecutive cycles with retention of 52% of the residual activity. The immobilized amylase produces high maltose syrup using the industrial wastewater containing corn starch. The ISO 5377 protocol determines dextrose equivalence values to be 36% and 24% during cycle 1 and cycle 2, respectively. The findings point out its possible commercial use.
摘要采用酪蛋白修饰的硅铁磁铁矿(fe3o4‐sio2)纳米颗粒固定化商用α‐淀粉酶,其中戊二醛作为交联剂。通过Box Behnken设计确定了酪蛋白(1.4%w/v)、fe3o4‐sio2(106µl)、戊二醛(55µl)和淀粉酶(1 mg/ml)等变量的最佳浓度。通过傅里叶变换红外光谱(FTIR)和热重分析(TGA)进一步证实了酪蛋白、戊二醛和酶在纳米颗粒上的结合。负载容量为37µg淀粉酶/ 1µg二氧化硅注入的磁铁矿纳米颗粒。催化可溶性淀粉酶和固定化淀粉酶的最佳pH值相同,即pH为7。然而,固定化后催化的pH范围得到改善。可溶性淀粉酶和固定化淀粉酶的最适温度分别为40℃和70℃。固定化后,淀粉酶的稳定性得到了提高,表现为半衰期的延长和失活速率常数的降低。固定化淀粉酶连续使用17个周期,剩余活性的保留率为52%。固定化淀粉酶利用含玉米淀粉的工业废水生产高麦芽糖糖浆。ISO 5377协议确定葡萄糖等效值在循环1和循环2期间分别为36%和24%。研究结果指出了它可能的商业用途。
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引用次数: 0
The Effect of Curcumin and Cornstarch Biopolymers on the Shelf Life of Fresh Cheese: Physicomechanical and Antimicrobial Properties 姜黄素和玉米淀粉生物聚合物对新鲜奶酪保质期的影响:物理、机械和抗菌性能
Pub Date : 2023-11-01 DOI: 10.1002/star.202300141
Yasir Qasim Almajidi, Andrew Ng Kay Lup, Andrés Alexis Ramírez‐Coronel, Abbas F. Almulla, Ali Alsudani, Mustafa Salam Kadhm, Yaser E. Alqurashi, Rasha Fadhel Obaid
Abstract Natural polymers are used more frequently in packaging as alternatives to synthetic plastics. Cornstarch is one of the cheapest carbohydrate biopolymers that form films with suitable properties and appearance but considerably larger hydrophilicity and low mechanical characteristics compared to synthetic films. Incorporating curcumin into cornstarch films improves mechanical and antimicrobial properties and curcumin release into aqueous solutions of the films. The results show that the introduction of curcumin decreases the water solubility of the edible films from 3.58% to 1.49% and the moisture absorption from 2.87% to 1.94% ( p < 0.05) and increases the length of films from 40% to 80% without changing their thickness. Examination of color properties shows that the increased curcumin concentration leads to a decrease in transparency and an increase in the redness and yellowness indices. These properties change from 72.2, 3.5, and 5.1 to 39.2, 21, and 13.7, respectively. The number of mold and yeast colonies in the cheese sample decreases during the storage period of 5 days. Generally, the findings indicate that the bioactive film of corn starch and curcumin (0.5%) can be used as a suitable coating for food products.
作为合成塑料的替代品,天然聚合物在包装中被更频繁地使用。玉米淀粉是最便宜的碳水化合物生物聚合物之一,它形成的薄膜具有合适的性能和外观,但与合成薄膜相比,亲水性大得多,机械特性低。将姜黄素掺入玉米淀粉薄膜中,可以改善薄膜的机械性能和抗菌性能,并使姜黄素释放到水溶液中。结果表明,姜黄素的引入使食用膜的水溶性由3.58%降至1.49%,吸湿率由2.87%降至1.94% (p <0.05),在不改变薄膜厚度的情况下,薄膜的长度从40%增加到80%。颜色性能测试表明,姜黄素浓度的增加导致透明度降低,红度和黄度指数增加。这些属性分别从72.2、3.5和5.1变为39.2、21和13.7。在5 d的贮藏期内,奶酪样品中的霉菌和酵母菌菌落数量减少。总的来说,研究结果表明,玉米淀粉和姜黄素(0.5%)的生物活性膜可以作为一种合适的食品涂层。
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引用次数: 0
Masthead: Starch ‐ Stärke 11–12/2023 刊头:Starch - Stärke 11-12/2023
Pub Date : 2023-11-01 DOI: 10.1002/star.202370026
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引用次数: 0
Contents: Starch ‐ Stärke 11–12/2023 内容:淀粉 - 强度 11-12/2023
Pub Date : 2023-11-01 DOI: 10.1002/star.202370027
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引用次数: 0
Editorial Board: Starch ‐ Stärke 11–12/2023 编辑委员会:淀粉 - 强度 11-12/2023
Pub Date : 2023-11-01 DOI: 10.1002/star.202370025
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引用次数: 0
Dual Starch Modifications to Expand Its End‐Uses: A Review 双重淀粉改性扩大其最终用途:综述
Pub Date : 2023-10-30 DOI: 10.1002/star.202300153
Carolina Lagunes‐Delgado, Edith Agama‐Acevedo, Luis A. Bello‐Pérez
Abstract Starch is a polysaccharide with diverse functionalities as a thickener and a gelling and water retention agent. These activities impact in the sensory, functional, and digestibility characteristics of the products where it is used as an ingredient. Native starch has diverse drawbacks, such as low resistance to acid conditions, syneresis, and sensitivity to high shear rates, among others. Those disadvantages are overcome by chemical, physical, and enzymatic modifications. In the last decade, the use of dual‐modified starch has been suggested to expand its applications, and eco‐friendly modifications are recommended. This review is designed to identify the effect of double modifications on starch structure, functionality, and digestibility characteristics considered by food manufacturers to develop new or improved products. The increase in the starch degree of substitution by dual modification is key to incipient applications, such as 3D printing, biodegradable packing materials with high mechanical resistance, and foods with slow resistance to enzymatic hydrolysis.
淀粉是一种具有多种功能的多糖,可作为增稠剂、胶凝剂和保水剂。当它被用作原料时,这些活动会影响产品的感官、功能和消化特性。天然淀粉有各种各样的缺点,比如对酸性条件的抵抗力低,协同作用,对高剪切速率的敏感性等等。这些缺点可以通过化学、物理和酶的修饰来克服。在过去的十年中,人们建议使用双改性淀粉来扩大其应用范围,并推荐生态友好型改性。本综述旨在确定双重改性对淀粉结构、功能和消化特性的影响,食品制造商在开发新的或改进的产品时考虑了双重改性。通过双重改性提高淀粉取代度是早期应用的关键,例如3D打印,具有高机械阻力的可生物降解包装材料,以及对酶水解的抵抗力较慢的食品。
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引用次数: 0
Cassava Root Cortex Peroxidase (CCP) as a Potential Alternative Source of Peroxidase 木薯根皮质过氧化物酶(CCP)作为过氧化物酶的潜在替代来源
Pub Date : 2023-10-28 DOI: 10.1002/star.202300083
Thakorn Sornwatana, Tiwa Rotchanapreeda, Rungdawan Wongsamart, Nadtaya Mongkol, Jamorn Somana
Abstract Cassava is an important starch crop in the world. Starch factories normally generate a huge amount of cassava root residual which may cause pollution to the environment. In order to find some extra uses for the root cortex, cassava root cortex peroxidase (CCP) is found in quantities up to 20 mg kg −1 in deteriorated cortex tissueand demonstrates some applications similar to horseradish peroxidase (HRP). The major native CCP is a 105‐kDa dimeric peroxidase with two 54‐kDa monomers. CCP is found to be tolerant to a broad pH range from 3 to 11 with maximum activity at pH 5.0 and maintains wide temperature range activity from 20 to 70 °C with an optimum at 65 °C. This indicates CCP to be one of the most robust peroxidases. Despite simple purification with ammonium sulfate precipitation, partial purified CCP is capable of determining glucose concentrations with glucose oxidase similar capability to HRP. For application as a reporter enzyme in immunoassays, the lab‐made secondary antibody conjugated with CCPsuccessfully detects the specific antigen in Western blot analysis using achemiluminescent substrate in the same way as HRP. From the properties and results of trial applications, CCP can be equally replaced in all applications of HRP. The enzyme should be found more potential applications to gain the acceptance. Since raw material for CCP is industrially massive, it should emphasize not only optimization of purification and process cost for industry, but also commercializeto the market.
木薯是世界上重要的淀粉作物。淀粉工厂通常会产生大量的木薯根残留物,这可能会对环境造成污染。为了找到根皮质的一些额外用途,木薯根皮质过氧化物酶(CCP)在变质的皮质组织中含量高达20 mg kg - 1,并显示出一些类似于辣根过氧化物酶(HRP)的应用。主要的天然CCP是一个105 - kDa二聚体过氧化物酶,具有两个54 - kDa单体。研究发现,CCP对pH值从3到11的广泛范围具有耐受性,在pH值为5.0时具有最大活性,在20至70°C范围内保持广泛的温度范围活性,在65°C时具有最佳活性。这表明CCP是最强大的过氧化物酶之一。尽管用硫酸铵沉淀法简单纯化,但部分纯化的CCP具有与HRP相似的葡萄糖氧化酶测定葡萄糖浓度的能力。作为免疫分析中的报告酶,实验室自制的与ccp偶联的二抗在Western blot分析中成功检测特异性抗原,使用与HRP相同的非致发光底物。从试验应用的性质和结果来看,CCP在HRP的所有应用中都是可以平等替代的。该酶需要寻找更多的潜在应用,才能获得认可。由于CCP的原料在工业上是大量的,因此在工业上不仅要注重提纯和工艺成本的优化,而且要注重市场化。
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引用次数: 0
Effects of Okara and Its Dietary Fiber on the Retrogradation and Rheological Characteristics of Potato Starch Okara及其膳食纤维对马铃薯淀粉退化及流变特性的影响
Pub Date : 2023-10-28 DOI: 10.1002/star.202300081
Yang Zhuang, Weina Xu, Yudong Wang, Hong Yang
Abstract The objective of this study is to investigate the effects of okara and its dietary fiber (DF) on the retrogradation and rheological characteristics of potato starch. DF or okara increases the syneresis, but decrease the gel strength of potato starch with the increase of concentration. Storage modulus and loss modulus of potato starch are markedly increased with a high concentration of okara or DF during temperature sweep, especially DF. Shear‐thinning behavior and apparent viscosity of potato starch are increased with more addition of DF or okara. The X‐ray diffraction pattern demonstrates that okara or DF does not change the type of crystal structure but significantly increases the crystallinity of the starch matrix, especially DF. Fourier‐transformed infrared spectra indicate the addition of okara or DF increases hydrogen bonds in potato starch and brings in ester carbonyl from the fiber. The addition of okara and DF can alter the retrogradation and rheology characteristics of potato starch.
摘要本试验旨在研究山核桃及其膳食纤维(DF)对马铃薯淀粉降解及流变特性的影响。随着浓度的增加,DF或okara增加了马铃薯淀粉的协同作用,但降低了凝胶强度。马铃薯淀粉的储存模量和损失模量在扫温过程中随高浓度的okara或DF而显著增加,尤其是DF。马铃薯淀粉的剪切减薄性能和表观粘度随着DF或okara添加量的增加而增加。X射线衍射图表明,okara或DF没有改变淀粉基质的晶体结构类型,但显著提高了淀粉基质的结晶度,尤其是DF。傅里叶变换红外光谱表明,添加okara或DF增加了马铃薯淀粉中的氢键,并从纤维中引入酯羰基。马铃薯淀粉的变性和流变性可以改变马铃薯淀粉的变性和流变性。
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引用次数: 0
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Starch - Stärke
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