首页 > 最新文献

Symbiosis最新文献

英文 中文
Disentangling the gut bacterial communities of the agave weevil, Scyphophorus acupunctatus (Coleoptera: Curculionidae) 解密龙舌兰象鼻虫(鞘翅目:蝼蛄)的肠道细菌群落
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-02-29 DOI: 10.1007/s13199-024-00978-4
Gabriela I. Salazar-Rivera, Alejandro Pereira-Santana, Ioreni Margarita Hernández-Velázquez, Mizraim Olivares-Miranda, Enrique Ibarra-Laclette, Anne C. Gschaedler Mathis, Jhony Navat Enríquez-Vara, Jesús Alejandro Zamora-Briseño

The agave weevil, Scyphophorus acupunctatus, is a pest of agave. Its larvae cause damage to agaves by boring holes in the plant. Boring requires that the insect consume the constituents of its host plant, which contains sugars and many recalcitrant polymers. It has been hypothesized for many years that the gut bacterial communities of S. acupunctatus play a role in its ability to metabolize agave components. However, studies exploring this insect's gut bacterial communities have yet to be performed. In this work, we used a 16S rRNA gene-based metabarcoding approach to characterize the gut bacterial communities of field-collected agave weevils from different localities in Mexico. We found that external factors, including host plants, have important effects on the structure of the gut bacterial communities of S. acupunctatus. Despite this variability, we found a discrete core bacterial community mainly composed of the genera Prevotella, Pectinatus, Liquorilactobacillus, Secundilactobacillus, Paucilactobacillus, and Pseudomonas. These genera may be necessary for S. acupunctatus as metabolic helpers and/or gatekeepers. Additional studies are needed to fully assess the functionality of the gut bacterial community of this species in terms of its metabolic contribution, which may help to decipher their potential ecological implications. The information we provided here is the first step for guiding further questions.

龙舌兰象鼻虫是龙舌兰的一种害虫。其幼虫在龙舌兰植物上钻孔,对龙舌兰造成损害。钻孔需要昆虫消耗寄主植物的成分,而寄主植物含有糖分和许多难降解的聚合物。多年来,人们一直假设穴居龙舌兰的肠道细菌群落对其代谢龙舌兰成分的能力有影响。然而,探索这种昆虫肠道细菌群落的研究尚未开展。在这项工作中,我们采用基于 16S rRNA 基因的代谢编码方法,描述了从墨西哥不同地区实地采集的龙舌兰象鼻虫肠道细菌群落的特征。我们发现,外部因素(包括寄主植物)对龙舌兰象鼻虫肠道细菌群落的结构有重要影响。尽管存在这种变异性,我们还是发现了一个离散的核心细菌群落,主要由普雷沃特氏菌属、果胶菌属、液乳杆菌属、半乳杆菌属、半乳杆菌属和假单胞菌属组成。这些菌属可能是穴居芽孢杆菌作为代谢助手和/或守门员所必需的。要全面评估该物种肠道细菌群落在新陈代谢方面的功能,还需要进行更多的研究,这可能有助于解读其潜在的生态影响。我们在此提供的信息是指导进一步问题研究的第一步。
{"title":"Disentangling the gut bacterial communities of the agave weevil, Scyphophorus acupunctatus (Coleoptera: Curculionidae)","authors":"Gabriela I. Salazar-Rivera, Alejandro Pereira-Santana, Ioreni Margarita Hernández-Velázquez, Mizraim Olivares-Miranda, Enrique Ibarra-Laclette, Anne C. Gschaedler Mathis, Jhony Navat Enríquez-Vara, Jesús Alejandro Zamora-Briseño","doi":"10.1007/s13199-024-00978-4","DOIUrl":"https://doi.org/10.1007/s13199-024-00978-4","url":null,"abstract":"<p>The agave weevil, <i>Scyphophorus acupunctatus</i>, is a pest of agave. Its larvae cause damage to agaves by boring holes in the plant. Boring requires that the insect consume the constituents of its host plant, which contains sugars and many recalcitrant polymers. It has been hypothesized for many years that the gut bacterial communities of <i>S. acupunctatus</i> play a role in its ability to metabolize agave components. However, studies exploring this insect's gut bacterial communities have yet to be performed. In this work, we used a 16S rRNA gene-based metabarcoding approach to characterize the gut bacterial communities of field-collected agave weevils from different localities in Mexico. We found that external factors, including host plants, have important effects on the structure of the gut bacterial communities of <i>S. acupunctatus</i>. Despite this variability, we found a discrete core bacterial community mainly composed of the genera <i>Prevotella</i>, <i>Pectinatus</i>, <i>Liquorilactobacillus</i>, <i>Secundilactobacillus</i>, <i>Paucilactobacillus,</i> and <i>Pseudomonas</i>. These genera may be necessary for <i>S. acupunctatus </i>as metabolic helpers and/or gatekeepers. Additional studies are needed to fully assess the functionality of the gut bacterial community of this species in terms of its metabolic contribution, which may help to decipher their potential ecological implications. The information we provided here is the first step for guiding further questions.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"5 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140007826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of symbiotic cyanobacteria in cycad coralloid roots using a short-read rbcL-X amplicon 利用短读数 rbcL-X 扩增子分析苏铁珊瑚根中共生蓝藻的多样性
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-02-21 DOI: 10.1007/s13199-024-00972-w
Adriel M. Sierra, Sandrine Toupin, Marta Alonso-García, Juan Carlos Villarreal A.

Cycads are the only gymnosperms forming a symbiosis with nitrogen-fixing cyanobacteria in a specialized organ: the coralloid root. This paper investigates the endophytic bacterial community inhabiting the coralloid roots of two cycads from Panama. We sampled coralloid roots from Zamia nana (terrestrial) and Zamia pseudoparasitica (epiphytic). Then, we used the 16S rRNA amplicon marker to describe the entire bacterial community. We also designed a new marker to amplify the rbcL-rbcX spacer and around 100 bp of the rbcX gene, targeting cyanobacteria. We found that using 16S, endophytic bacteria diversity is represented mainly by the phyla Actinobacteria, Cyanobacteria, and Proteobacteria. In addition, 16S analyses showed that Zamia species do not share a core cyanobacterial community (using stringent 75% and 90% thresholds), while the two species shared 4 ASVs at a 50% threshold. The newly developed rbcL-rbcX marker revealed that both species share a core cyanobacterial community represented by a single amplicon sequence variant (ASV1) (Nostoc sp.) at 90% threshold that is found in the same phylogenetic clade of that contain mostly Panamanian symbiotic cyanobacteria. Using a 75% threshold, only three ASVs (ASV1, ASV2, ASV3) were present across samples, and five ASVs at 50% threshold. This new marker can effectively identify cyanobacteria ASVs and provide a better resolution for microbial analyses in autotroph cyanobacterial symbioses.

苏铁是唯一与固氮蓝藻在珊瑚根这一特殊器官中形成共生关系的裸子植物。本文研究了栖息在巴拿马两棵苏铁珊瑚根中的内生细菌群落。我们对 Zamia nana(陆生)和 Zamia pseudoparasitica(附生)的珊瑚状根进行了取样。然后,我们使用 16S rRNA 扩增子标记来描述整个细菌群落。我们还针对蓝藻设计了一种新的标记,用于扩增 rbcL-rbcX spacer 和 rbcX 基因的约 100 bp。我们发现,利用 16S,内生细菌的多样性主要体现在放线菌门、蓝藻门和蛋白菌门。此外,16S 分析表明 Zamia 物种并不共享核心蓝藻群落(使用严格的 75% 和 90% 临界值),而在 50% 临界值下,两个物种共享 4 个 ASV。新开发的 rbcL-rbcX 标记显示,这两个物种共享一个核心蓝藻群落,在 90% 的阈值下,该群落由一个单一的扩增子序列变体(ASV1)(Nostoc sp.)代表。以 75% 的阈值计算,各样本中只存在三个 ASV(ASV1、ASV2、ASV3),以 50% 的阈值计算则存在五个 ASV。这种新标记能有效识别蓝藻 ASV,并为自养蓝藻共生体的微生物分析提供更高的分辨率。
{"title":"Diversity of symbiotic cyanobacteria in cycad coralloid roots using a short-read rbcL-X amplicon","authors":"Adriel M. Sierra, Sandrine Toupin, Marta Alonso-García, Juan Carlos Villarreal A.","doi":"10.1007/s13199-024-00972-w","DOIUrl":"https://doi.org/10.1007/s13199-024-00972-w","url":null,"abstract":"<p>Cycads are the only gymnosperms forming a symbiosis with nitrogen-fixing cyanobacteria in a specialized organ: the coralloid root. This paper investigates the endophytic bacterial community inhabiting the coralloid roots of two cycads from Panama. We sampled coralloid roots from <i>Zamia nana</i> (terrestrial) and <i>Zamia pseudoparasitica</i> (epiphytic). Then, we used the 16S rRNA amplicon marker to describe the entire bacterial community. We also designed a new marker to amplify the <i>rbc</i>L-<i>rbc</i>X spacer and around 100 bp of the <i>rbc</i>X gene, targeting cyanobacteria. We found that using 16S, endophytic bacteria diversity is represented mainly by the phyla Actinobacteria, Cyanobacteria, and Proteobacteria. In addition, 16S analyses showed that <i>Zamia</i> species do not share a core cyanobacterial community (using stringent 75% and 90% thresholds), while the two species shared 4 ASVs at a 50% threshold. The newly developed <i>rbc</i>L-<i>rbc</i>X marker revealed that both species share a core cyanobacterial community represented by a single amplicon sequence variant (ASV1) (<i>Nostoc sp.</i>) at 90% threshold that is found in the same phylogenetic clade of that contain mostly Panamanian symbiotic cyanobacteria. Using a 75% threshold, only three ASVs (ASV1, ASV2, ASV3) were present across samples, and five ASVs at 50% threshold. This new marker can effectively identify cyanobacteria ASVs and provide a better resolution for microbial analyses in autotroph cyanobacterial symbioses.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"28-29 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139921901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
How do phoront density and anuran host traits affect phoresy? 噬菌体密度和无尾类宿主特征如何影响噬菌体?
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-02-19 DOI: 10.1007/s13199-024-00979-3
Alan Pedro de Araújo, Rodrigo Barbosa Ferreira, Emanuel Giovane Cafofo, Cássio Zocca, Rogério Pereira Bastos

Phoresy is likely a commensal interaction, in which a phoront attaches itself to the body of a host for dispersal. Host traits and environmental characteristics may determine phoresy patterns. We examined whether the density of microscopic phoronts on frogs living in the water between the leaves of bromeliads (phytotelma) was determined by the species, richness, size and abundance of anurans (frog hosts). We identified phoronts on the skin of three hylid species (Dendropsophus bromeliaceus, Phyllodytes luteolus, and Ololygon arduoa) associated to bromeliads at eight sites in the Atlantic Forest of Brazil. We report the first records of phoretic interaction between these bromeligenous frog hosts with the aquatic invertebrates of bromeliads e.g., Bdelloidea, Copepoda, Acari, and Ostracoda. The size of frog hosts had negative relationship with the abundance of phoronts. However, when the species were analyzed separately, the size of O. arduoa had positive relationship with the abundance of phoronts. The richness of anurans in bromeliads did not influence phoresy in any of the observed variables. Our results showed that host attributes, such as identity, size, and abundance, as well as the density of phoronts in their habitat, influenced the phoresy rates. This implies that not only host attributes, but also those of the phoront need to be considered in phoresy studies.

噬菌体很可能是一种共生互动,在这种互动中,噬菌体附着在宿主身上进行传播。宿主的特性和环境特征可能会决定噬菌体的模式。我们研究了生活在凤梨(凤梨属植物)叶片之间的水中的青蛙身上的微小噬菌体密度是否取决于无尾目动物(青蛙宿主)的种类、丰富程度、大小和数量。我们在巴西大西洋森林的八个地点发现了与凤梨相关的三种蛙类(Dendropsophus bromeliaceus、Phyllodytes luteolus 和 Ololygon arduoa)皮肤上的噬菌体。我们首次报告了这些凤梨蛙宿主与凤梨属水生无脊椎动物(如栉水母纲、桡足纲、蛔虫纲和梭形纲)之间的孢子相互作用。蛙类寄主的大小与噬菌体的数量呈负相关。然而,当对物种进行单独分析时,O. arduoa 的大小与噬菌体的数量呈正相关。凤梨科植物中无柄类动物的丰富程度并不影响任何观测变量的噬菌体数量。我们的研究结果表明,寄主的特性(如身份、大小和丰度)以及栖息地的噬菌体密度都会影响噬菌率。这意味着,在进行幻食研究时,不仅需要考虑宿主的属性,还需要考虑噬菌体的属性。
{"title":"How do phoront density and anuran host traits affect phoresy?","authors":"Alan Pedro de Araújo, Rodrigo Barbosa Ferreira, Emanuel Giovane Cafofo, Cássio Zocca, Rogério Pereira Bastos","doi":"10.1007/s13199-024-00979-3","DOIUrl":"https://doi.org/10.1007/s13199-024-00979-3","url":null,"abstract":"<p>Phoresy is likely a commensal interaction, in which a phoront attaches itself to the body of a host for dispersal. Host traits and environmental characteristics may determine phoresy patterns. We examined whether the density of microscopic phoronts on frogs living in the water between the leaves of bromeliads (phytotelma) was determined by the species, richness, size and abundance of anurans (frog hosts). We identified phoronts on the skin of three hylid species (<i>Dendropsophus bromeliaceus</i>, <i>Phyllodytes luteolus</i>, and <i>Ololygon arduoa</i>) associated to bromeliads at eight sites in the Atlantic Forest of Brazil. We report the first records of phoretic interaction between these bromeligenous frog hosts with the aquatic invertebrates of bromeliads e.g., Bdelloidea, Copepoda, Acari, and Ostracoda. The size of frog hosts had negative relationship with the abundance of phoronts. However, when the species were analyzed separately, the size of <i>O. arduoa</i> had positive relationship with the abundance of phoronts. The richness of anurans in bromeliads did not influence phoresy in any of the observed variables. Our results showed that host attributes, such as identity, size, and abundance, as well as the density of phoronts in their habitat, influenced the phoresy rates. This implies that not only host attributes, but also those of the phoront need to be considered in phoresy studies.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"1 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139902069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soil microbial communities alter resource allocation in Fagus grandifolia when challenged with a pathogen 土壤微生物群落在受到病原体挑战时会改变红叶石楠的资源分配
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-27 DOI: 10.1007/s13199-023-00969-x
Sarah R. Carrino-Kyker, Anna L. Parker, Juliana S. Medeiros, Charlotte R. Hewins, Glenn R. Novotny, Stephen L. Krebs, David J. Burke

Soil microbes are influenced by their environment, and soil pH is well known as a driver of community structure, including within the plant root zone. However, the effect of pH induced changes on root-associated microbial communities for plant growth, resource allocation, and disease resistance is not well understood, especially for long-lived woody plants. In this study, we examined whether soil microbial communities altered by soil pH could affect tree growth, resource allocation, and resistance to a soil-borne pathogen. In a controlled greenhouse setting, we treated Fagus grandifolia saplings with small amounts of forest soil that had been manipulated to alter soil pH and microbial communities. In addition, 1-yr after inoculation with forest soil, half of the trees were also inoculated with the root rot pathogen Phytophthora cinnamomi to induce physiological stress. Tree growth showed no response to treatment with forest microbes; however, P. cinnamomi altered resource allocation, leading to increased ratios of aboveground to belowground biomass for trees treated with forest microbes. Interestingly, trees grown in pasteurized soil had a tendency toward the opposite pattern of reduced ratios of aboveground to belowground biomass. Soil treatment and pathogen inoculation interacted to alter water transport tissues; stems grown with microbes from acidic forest soil had higher vessel density when challenged with P. cinnamomi, while trees grown with microbes from neutral forest soil had higher vessel density in the absence of the pathogen. Our study suggests that the composition of root-associated microbes can affect resource allocation under stressful conditions for long-lived woody plants.

土壤微生物受到环境的影响,众所周知,土壤 pH 值是群落结构(包括植物根区)的驱动因素。然而,pH 值的变化对植物生长、资源分配和抗病性的根相关微生物群落的影响还不甚了解,尤其是对长寿木本植物而言。在这项研究中,我们考察了土壤微生物群落是否会因土壤 pH 值的改变而影响树木的生长、资源分配和对土传病原体的抵抗力。在受控的温室环境中,我们用少量经过处理的森林土壤处理法桐树苗,以改变土壤 pH 值和微生物群落。此外,在接种森林土壤 1 年后,一半的树木还接种了根腐病病原体 Phytophthora cinnamomi,以诱发生理压力。树木的生长对森林微生物的处理没有反应;然而,P. cinnamomi 改变了资源分配,导致使用森林微生物处理的树木的地上生物量与地下生物量之比增加。有趣的是,在巴氏杀菌法土壤中生长的树木则倾向于相反的模式,即地下生物量与地上生物量之比降低。土壤处理和病原体接种相互影响,改变了水分运输组织;用酸性森林土壤中的微生物培育的茎干在受到 P. cinnamomi 的挑战时血管密度更高,而用中性森林土壤中的微生物培育的树木在没有病原体的情况下血管密度更高。我们的研究表明,根相关微生物的组成会影响长寿木本植物在压力条件下的资源分配。
{"title":"Soil microbial communities alter resource allocation in Fagus grandifolia when challenged with a pathogen","authors":"Sarah R. Carrino-Kyker, Anna L. Parker, Juliana S. Medeiros, Charlotte R. Hewins, Glenn R. Novotny, Stephen L. Krebs, David J. Burke","doi":"10.1007/s13199-023-00969-x","DOIUrl":"https://doi.org/10.1007/s13199-023-00969-x","url":null,"abstract":"<p>Soil microbes are influenced by their environment, and soil pH is well known as a driver of community structure, including within the plant root zone. However, the effect of pH induced changes on root-associated microbial communities for plant growth, resource allocation, and disease resistance is not well understood, especially for long-lived woody plants. In this study, we examined whether soil microbial communities altered by soil pH could affect tree growth, resource allocation, and resistance to a soil-borne pathogen. In a controlled greenhouse setting, we treated <i>Fagus grandifolia</i> saplings with small amounts of forest soil that had been manipulated to alter soil pH and microbial communities. In addition, 1-yr after inoculation with forest soil, half of the trees were also inoculated with the root rot pathogen <i>Phytophthora cinnamomi</i> to induce physiological stress<i>.</i> Tree growth showed no response to treatment with forest microbes; however, <i>P. cinnamomi</i> altered resource allocation, leading to increased ratios of aboveground to belowground biomass for trees treated with forest microbes. Interestingly, trees grown in pasteurized soil had a tendency toward the opposite pattern of reduced ratios of aboveground to belowground biomass. Soil treatment and pathogen inoculation interacted to alter water transport tissues; stems grown with microbes from acidic forest soil had higher vessel density when challenged with <i>P. cinnamomi</i>, while trees grown with microbes from neutral forest soil had higher vessel density in the absence of the pathogen. Our study suggests that the composition of root-associated microbes can affect resource allocation under stressful conditions for long-lived woody plants.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"13 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139578973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved use of mycorrhizal fungi for upscaling of orchid cultivation and population restoration of endangered orchids in China 提高菌根真菌在中国兰花栽培和濒危兰花种群恢复中的利用率
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-24 DOI: 10.1007/s13199-024-00974-8
Shi-Cheng Shao, Hans Jacquemyn, Marc-Andre Selosse

The orchid family (Orchidaceae) is one of the most diverse plant families in the world, but at the same time also contains one of the largest number of rare and endangered species. While conservation actions such as in situ and ex situ conservation and prohibition of international trade of wild orchids have achieved positive results to slow down the current decline of orchids, species with high medicinal or ornamental value may require more advanced measures. Recent pilot studies using novel cultivation techniques have successfully reintroduced endangered epiphytic orchids and facilitated the commercial cultivation of medicinal orchids. Because fungal partners play a key role in in situ symbiotic seed germination and industrial cultivation, we advocate for the development of fungus banks in laboratories engaging in orchid research, where fungi obtained from germinating seeds or seedlings can be studied and used to improve orchid germination under both in vitro and in situ conditions. Furthermore, these fungi could be shared nationally and internationally, enhancing orchid conservation efforts across the globe. Similar to seed banks, the development of fungus banks will reduce the possibility of fungi going extinct and ensure their availability for reintroduction programs and commercial cultivation. With the availability of both a fungus and seed bank, the conservation of threatened orchid species can be significantly enhanced by improving restoration programs and commercial cultivation of medicinal orchids and releasing the pressure on wild populations from harvesting.

兰科(Orchidaceae)是世界上最多样化的植物科之一,但同时也是稀有和濒危物种数量最多的植物科之一。虽然原地和异地保护以及禁止野生兰花国际贸易等保护行动已经取得了积极成果,减缓了兰花目前的衰退速度,但药用或观赏价值较高的物种可能需要采取更先进的措施。最近,利用新型栽培技术进行的试点研究成功地重新引入了濒危附生兰,并促进了药用兰花的商业化栽培。由于真菌伙伴在原生境共生种子萌发和工业化栽培中发挥着关键作用,我们主张在从事兰花研究的实验室中建立真菌库,研究从萌发种子或幼苗中获得的真菌,并将其用于提高兰花在体外和原生境条件下的萌发能力。此外,这些真菌还可以在国内和国际上共享,从而加强全球兰花保护工作。与种子库类似,真菌库的发展将减少真菌灭绝的可能性,并确保它们可用于重新引入计划和商业栽培。有了真菌库和种子库,就可以通过改善药用兰花的恢复计划和商业栽培,缓解野生种群的采摘压力,从而大大加强对受威胁兰花物种的保护。
{"title":"Improved use of mycorrhizal fungi for upscaling of orchid cultivation and population restoration of endangered orchids in China","authors":"Shi-Cheng Shao, Hans Jacquemyn, Marc-Andre Selosse","doi":"10.1007/s13199-024-00974-8","DOIUrl":"https://doi.org/10.1007/s13199-024-00974-8","url":null,"abstract":"<p>The orchid family (Orchidaceae) is one of the most diverse plant families in the world, but at the same time also contains one of the largest number of rare and endangered species. While conservation actions such as <i>in situ</i> and <i>ex situ</i> conservation and prohibition of international trade of wild orchids have achieved positive results to slow down the current decline of orchids, species with high medicinal or ornamental value may require more advanced measures. Recent pilot studies using novel cultivation techniques have successfully reintroduced endangered epiphytic orchids and facilitated the commercial cultivation of medicinal orchids. Because fungal partners play a key role in <i>in situ</i> symbiotic seed germination and industrial cultivation, we advocate for the development of fungus banks in laboratories engaging in orchid research, where fungi obtained from germinating seeds or seedlings can be studied and used to improve orchid germination under both <i>in vitro</i> and <i>in situ</i> conditions. Furthermore, these fungi could be shared nationally and internationally, enhancing orchid conservation efforts across the globe. Similar to seed banks, the development of fungus banks will reduce the possibility of fungi going extinct and ensure their availability for reintroduction programs and commercial cultivation. With the availability of both a fungus and seed bank, the conservation of threatened orchid species can be significantly enhanced by improving restoration programs and commercial cultivation of medicinal orchids and releasing the pressure on wild populations from harvesting.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"7 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139557698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parasites or predators? Gastropod ectoparasites and their scleractinian host corals at Koh Tao, Gulf of Thailand, with the description of a new species 寄生虫还是捕食者?泰国湾涛岛的腹足类外寄生虫及其硬骨鱼寄主珊瑚,并描述一个新物种
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-24 DOI: 10.1007/s13199-023-00967-z

Abstract

Recent years have seen a rapid increase in the study of coral-associated gastropods. In particular, the description of several new species in conjunction with their host specificity or dietary variability, has raised questions pertaining to their impact on reef health. These corallivores have been labelled as both ‘parasite’ and ‘predator’ by different studies, due to the tendency of some species to entirely consume their ‘host’ corals. Here we present new findings of corallivory and parasitism based on surveys conducted on the reefs of Koh Tao, Gulf of Thailand. A total of 6566 corals were assessed for their tendency to host gastropods of the nudibranch genus Phestilla and the caenogastropod family Epitoniidae. Thirteen gastropod species were found to be associated with 20 scleractinian coral species, including six that do not match the original description of previously known taxa. Herein we describe one of them, the first nudibranch proven to be associated with corals of the scleractinian genus Acropora and discuss conservation implications of these coral/gastropod relationships. Additionally, we explore the complex topic of defining these relationships as parasitic versus predatory and the merits of using these labels to better understand the ecology of these relationships.

摘要 近年来,对珊瑚相关腹足类动物的研究迅速增加。特别是对一些新物种的描述,以及它们的寄主特异性或食物变异性,引起了有关它们对珊瑚礁健康影响的问题。由于一些物种倾向于完全吃掉它们的 "宿主 "珊瑚,这些珊瑚食肉动物在不同的研究中被称为 "寄生虫 "和 "捕食者"。在此,我们根据对泰国湾涛岛珊瑚礁的调查,介绍了珊瑚食性和寄生性的新发现。我们对 6566 个珊瑚进行了评估,以确定它们是否会寄生于裸鳃珊瑚属(Phestilla)和腹足纲(Epitoniidae)腹足目动物。结果发现,有 13 个腹足类动物与 20 个硬骨珊瑚物种相关,其中有 6 个与以前已知类群的原始描述不符。在本文中,我们描述了其中的一个物种--第一个被证实与硬骨珊瑚属 Acropora 相关的裸鳃类,并讨论了这些珊瑚/腹足类关系对保护的影响。此外,我们还探讨了将这些关系定义为寄生性还是捕食性的复杂问题,以及使用这些标签来更好地理解这些关系的生态学的优点。
{"title":"Parasites or predators? Gastropod ectoparasites and their scleractinian host corals at Koh Tao, Gulf of Thailand, with the description of a new species","authors":"","doi":"10.1007/s13199-023-00967-z","DOIUrl":"https://doi.org/10.1007/s13199-023-00967-z","url":null,"abstract":"<h3>Abstract</h3> <p>Recent years have seen a rapid increase in the study of coral-associated gastropods. In particular, the description of several new species in conjunction with their host specificity or dietary variability, has raised questions pertaining to their impact on reef health. These corallivores have been labelled as both ‘parasite’ and ‘predator’ by different studies, due to the tendency of some species to entirely consume their ‘host’ corals. Here we present new findings of corallivory and parasitism based on surveys conducted on the reefs of Koh Tao, Gulf of Thailand. A total of 6566 corals were assessed for their tendency to host gastropods of the nudibranch genus <em>Phestilla</em> and the caenogastropod family Epitoniidae. Thirteen gastropod species were found to be associated with 20 scleractinian coral species, including six that do not match the original description of previously known taxa. Herein we describe one of them, the first nudibranch proven to be associated with corals of the scleractinian genus <em>Acropora</em> and discuss conservation implications of these coral/gastropod relationships. Additionally, we explore the complex topic of defining these relationships as parasitic versus predatory and the merits of using these labels to better understand the ecology of these relationships.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"7 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139557509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monocropping and Intercropping of Maize with Six Food Legumes at Malkerns in Eswatini: Their Effects on Plant Growth, Grain Yield and N2 Fixation, Measured using the 15N Natural Abundance and Ureide Techniques 在埃斯瓦提尼的马尔克斯,玉米与六种食用豆科植物的单作和间作:利用 15N 自然丰度和 Ureide 技术测量它们对植物生长、谷物产量和 N2 固定的影响
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-22 DOI: 10.1007/s13199-024-00971-x
Zanele D. Ngwenya, Mustapha Mohammed, Felix D. Dakora

Intercropping of legumes and cereals has many benefits to both plant partners. In this study, the effect of legume-maize intercropping on plant growth, grain yield and N2 fixation of six legumes was assessed using the 15N natural abundance and ureide techniques. For this, a field experiment involving six legume species and two cropping systems was established at the Malkerns Research Station, Eswatini during the 2017/2018 cropping season. Based on the 15N isotopic and ureide analysis, the six test legumes respectively obtained 39.06 – 70.19% and 16.46 – 55.79% of their N nutrition from symbiosis. The amounts of N-fixed ranged from 12.66 to 66.57 kg ha−1. In general, high amount of N-fixed by legumes correlated strongly with greater shoot dry matter accumulation (r = 0.7981; p < 0.001) and high grain yield (r = 0.5905; p < 0.001), indicating the importance of N2 fixation in plant growth and reproduction. Legumes grown under monocropping recorded higher plant growth, symbiotic performance and grain yield when compared to those grown in mixed culture with maize. However, shoot %Ndfa was much higher under intercropping than sole cropping due to competition by cereal and legume for soil N. Components of maize yield were similar for the two cropping systems. The %N derived from fixation and %relative ureide-N abundance were significantly correlated (r = 0.4005; p < 0.001), indicating that the 15N natural abundance technique and the ureide method were complementary in measuring N2 fixation in the test legumes. These results have provided some insights on the impact of cropping system on plant growth, symbiotic performance and grain yield of six selected legumes.

豆科植物与谷物间作对植物伙伴都有很多好处。本研究利用 15N 自然丰度和尿苷技术评估了豆科-玉米间作对植物生长、谷物产量和六种豆科植物的氮固定的影响。为此,在2017/2018种植季期间,在埃斯瓦提尼的马尔克恩斯研究站开展了一项涉及六种豆科植物和两种种植系统的田间试验。根据 15N 同位素和尿苷分析,六种试验豆科植物分别从共生中获得了 39.06% - 70.19% 和 16.46% - 55.79% 的氮营养。固定的氮量范围为 12.66 至 66.57 千克/公顷。一般来说,豆科植物的高氮固定量与较高的嫩枝干物质积累(r = 0.7981; p < 0.001)和较高的谷物产量(r = 0.5905; p < 0.001)密切相关,表明氮固定在植物生长和繁殖中的重要性。与与玉米混合栽培的豆科植物相比,单作豆科植物的植株生长、共生性能和谷物产量更高。然而,由于谷物和豆科植物对土壤氮的竞争,间作种植下的嫩枝氮固定率(%Ndfa)远高于单作种植下的嫩枝氮固定率(%Ndfa)。固定产生的氮的百分比与尿素氮丰度的百分比显著相关(r = 0.4005; p < 0.001),表明 15N 自然丰度技术和尿素法在测量试验豆科植物的 N2 固定方面具有互补性。这些结果为研究耕作制度对六种选定豆科植物的生长、共生性能和谷物产量的影响提供了一些启示。
{"title":"Monocropping and Intercropping of Maize with Six Food Legumes at Malkerns in Eswatini: Their Effects on Plant Growth, Grain Yield and N2 Fixation, Measured using the 15N Natural Abundance and Ureide Techniques","authors":"Zanele D. Ngwenya, Mustapha Mohammed, Felix D. Dakora","doi":"10.1007/s13199-024-00971-x","DOIUrl":"https://doi.org/10.1007/s13199-024-00971-x","url":null,"abstract":"<p>Intercropping of legumes and cereals has many benefits to both plant partners. In this study, the effect of legume-maize intercropping on plant growth, grain yield and N<sub>2</sub> fixation of six legumes was assessed using the <sup>15</sup>N natural abundance and ureide techniques. For this, a field experiment involving six legume species and two cropping systems was established at the Malkerns Research Station, Eswatini during the 2017/2018 cropping season. Based on the <sup>15</sup>N isotopic and ureide analysis, the six test legumes respectively obtained 39.06 – 70.19% and 16.46 – 55.79% of their N nutrition from symbiosis. The amounts of N-fixed ranged from 12.66 to 66.57 kg ha<sup>−1</sup>. In general, high amount of N-fixed by legumes correlated strongly with greater shoot dry matter accumulation (r = 0.7981; p &lt; 0.001) and high grain yield (r = 0.5905; p &lt; 0.001), indicating the importance of N<sub>2</sub> fixation in plant growth and reproduction. Legumes grown under monocropping recorded higher plant growth, symbiotic performance and grain yield when compared to those grown in mixed culture with maize. However, shoot %Ndfa was much higher under intercropping than sole cropping due to competition by cereal and legume for soil N. Components of maize yield were similar for the two cropping systems. The %N derived from fixation and %relative ureide-N abundance were significantly correlated (r = 0.4005; p &lt; 0.001), indicating that the <sup>15</sup>N natural abundance technique and the ureide method were complementary in measuring N<sub>2</sub> fixation in the test legumes. These results have provided some insights on the impact of cropping system on plant growth, symbiotic performance and grain yield of six selected legumes.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"191 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139518192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Obligate mutualistic heritable symbiosis in sap-feeding insects: an intricate relationship in nature 食液昆虫的义务性互利遗传共生:自然界中错综复杂的关系
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-22 DOI: 10.1007/s13199-023-00965-1

Abstract

Obligate endosymbionts have a significant impact on the physiology and ecology of their insect hosts and consequently have played an important role in their diversification and evolution. Auchenorrhyncha is a sap-feeding insect group that includes cicadas, spittlebugs, leafhoppers, treehoppers, and planthoppers, some of which are well-studied vectors of plant pathogens causing important diseases. Here, we review the obligate symbiotic systems in Auchenorrhyncha. First, we address the diversity of obligate endosymbionts, illustrating the complex scenario characterised by replacements, new acquisitions, and loss of endosymbionts along evolution. Then, we describe the mechanisms that allowed maintaining these long-term associations. Also, we attend to the functional interdependence between host and obligate endosymbionts and how insect hosts support and regulate them. Moreover, we discuss current research that considers the disruption of host-endosymbionts associations as a novel strategy to control these sap-feeding insects. Finally, we suggest directions for further studies regarding obligate mutualistic relationships as well as other symbiotic systems that could be helpful in increasing the knowledge of the complex interactions between Auchenorrhyncha and their associated microbes.

摘要 Obligate endosymbionts 对昆虫宿主的生理和生态有重大影响,因此在昆虫的多样化和进化过程中发挥了重要作用。Auchenorrhyncha是一个吸食汁液的昆虫类群,包括蝉、唾液虫、叶蝉、树蝉和刨花蝇,其中一些是植物病原体的载体,可引起重要的疾病。在此,我们将回顾 Auchenorrhyncha 的强制性共生系统。首先,我们探讨了强制性内生共生体的多样性,说明了内生共生体在进化过程中的替换、新获得和丧失等复杂情况。然后,我们描述了维持这些长期联系的机制。此外,我们还关注宿主与强制性内生共生体之间的功能相互依存关系,以及昆虫宿主如何支持和调节它们。此外,我们还讨论了当前的一些研究,这些研究认为破坏宿主与内生共生体之间的联系是控制这些食液昆虫的一种新策略。最后,我们提出了进一步研究强制性互惠关系和其他共生系统的方向,这些研究可能有助于加深对Auchenorrhyncha及其相关微生物之间复杂互动关系的了解。
{"title":"Obligate mutualistic heritable symbiosis in sap-feeding insects: an intricate relationship in nature","authors":"","doi":"10.1007/s13199-023-00965-1","DOIUrl":"https://doi.org/10.1007/s13199-023-00965-1","url":null,"abstract":"<h3>Abstract</h3> <p>Obligate endosymbionts have a significant impact on the physiology and ecology of their insect hosts and consequently have played an important role in their diversification and evolution. Auchenorrhyncha is a sap-feeding insect group that includes cicadas, spittlebugs, leafhoppers, treehoppers, and planthoppers, some of which are well-studied vectors of plant pathogens causing important diseases. Here, we review the obligate symbiotic systems in Auchenorrhyncha. First, we address the diversity of obligate endosymbionts, illustrating the complex scenario characterised by replacements, new acquisitions, and loss of endosymbionts along evolution. Then, we describe the mechanisms that allowed maintaining these long-term associations. Also, we attend to the functional interdependence between host and obligate endosymbionts and how insect hosts support and regulate them. Moreover, we discuss current research that considers the disruption of host-endosymbionts associations as a novel strategy to control these sap-feeding insects. Finally, we suggest directions for further studies regarding obligate mutualistic relationships as well as other symbiotic systems that could be helpful in increasing the knowledge of the complex interactions between Auchenorrhyncha and their associated microbes.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"54 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139517966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wolbachia-density variation in weevils of the tribe Naupactini (Coleoptera, Curculionidae) 象鼻虫(鞘翅目,虫科)中的沃尔巴克氏体密度变异
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-20 DOI: 10.1007/s13199-023-00963-3
Lucía da Cruz Cabral, Lucía Fernandez Goya, Romina V. Piccinali, Analía A. Lanteri, Viviana A. Confalonieri, Marcela S. Rodriguero

The intracellular bacteria Wolbachia pipientis infects arthropods and filarial nematodes and is able to manipulate host reproduction. It has been reported an association between parthenogenesis and Wolbachia infection in weevils from the tribe Naupactini. A curing experiment suggested that a threshold density of Wolbachia is required for parthenogenetic reproduction to occur. The aim of this study was to analyze Wolbachia infection status and density in two sexually reproducing species from the tribe Naupactini, Naupactus xanthographus and Naupactus dissimulator. Wolbachia infection was detected in individuals from both species in several geographic locations, not being fixed. Quantification through real time PCR confirmed that Wolbachia loads in sexual species were significantly lower than in parthenogenetic ones; these results support the hypothesis of a threshold level for parthenogenetic reproduction to occur in Naupactini weevils. Strain typing showed that both sexual species carry wNau1, the most frequent strain in parthenogenetic Naupactini weevils. In addition, the presence of the WO phage, which might be an important factor regulating infection density in some hosts, was detected in this strain. Finally, Wolbachia wNau1 was located throughout the whole insect body, which is in agreement with the idea of a recent acquisition by horizontal transfer of wNau1 across the tribe Naupactini.

胞内细菌 Wolbachia pipientis 感染节肢动物和丝虫线虫,能够操纵宿主的繁殖。据报道,在象鼻虫科(Naupactini)中,孤雌生殖与 Wolbachia 感染有关。一项固化实验表明,孤雌生殖的发生需要一定密度的沃尔巴克氏体。本研究旨在分析两种有性生殖的象鼻虫(Naupactini),即Naupactus xanthographus和Naupactus dissimulator的沃尔巴克氏体感染状况和密度。这两个物种的个体在多个地理位置都检测到了沃尔巴克氏体感染,但未被固定。通过实时聚合酶链式反应(real time PCR)定量证实,有性物种中的沃尔巴克氏体载量明显低于孤雌生殖的物种;这些结果支持了关于Naupactini象鼻虫孤雌生殖发生的阈值水平的假设。菌株分型结果表明,有性种和无性种都携带 wNau1,这是孤雌生殖象鼻虫中最常见的菌株。此外,在该菌株中还发现了 WO 噬菌体,它可能是调节某些宿主感染密度的重要因素。最后,Wolbachia wNau1遍布整个昆虫身体,这与最近在Naupactini象鼻虫中通过水平转移获得wNau1的观点一致。
{"title":"Wolbachia-density variation in weevils of the tribe Naupactini (Coleoptera, Curculionidae)","authors":"Lucía da Cruz Cabral, Lucía Fernandez Goya, Romina V. Piccinali, Analía A. Lanteri, Viviana A. Confalonieri, Marcela S. Rodriguero","doi":"10.1007/s13199-023-00963-3","DOIUrl":"https://doi.org/10.1007/s13199-023-00963-3","url":null,"abstract":"<p>The intracellular bacteria <i>Wolbachia pipientis</i> infects arthropods and filarial nematodes and is able to manipulate host reproduction. It has been reported an association between parthenogenesis and <i>Wolbachia</i> infection in weevils from the tribe Naupactini. A curing experiment suggested that a threshold density of <i>Wolbachia</i> is required for parthenogenetic reproduction to occur. The aim of this study was to analyze <i>Wolbachia</i> infection status and density in two sexually reproducing species from the tribe Naupactini, <i>Naupactus xanthographus</i> and <i>Naupactus dissimulator</i>. <i>Wolbachia</i> infection was detected in individuals from both species in several geographic locations, not being fixed. Quantification through real time PCR confirmed that <i>Wolbachia</i> loads in sexual species were significantly lower than in parthenogenetic ones; these results support the hypothesis of a threshold level for parthenogenetic reproduction to occur in Naupactini weevils. Strain typing showed that both sexual species carry <i>w</i>Nau1, the most frequent strain in parthenogenetic Naupactini weevils. In addition, the presence of the WO phage, which might be an important factor regulating infection density in some hosts, was detected in this strain. Finally, <i>Wolbachia w</i>Nau1 was located throughout the whole insect body, which is in agreement with the idea of a recent acquisition by horizontal transfer of <i>w</i>Nau1 across the tribe Naupactini.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"1 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139508035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dining on corals: stable isotope evidence for close trophic connection between gall crabs (Cryptochiridae) and their stony coral hosts 以珊瑚为食:五倍子蟹(隐鞭虫科)与其石珊瑚宿主之间密切营养联系的稳定同位素证据
IF 2.5 3区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-01-19 DOI: 10.1007/s13199-023-00968-y
Henrique Bravo, Charlotte R. Dromard, Marcel T. J. van der Meer, Anna Schleimer, Sancia E. T. van der Meij

The exact nature of the relationship between symbiont fauna and their hosts is often unclear, but knowing more about these intricate ecological interactions is vital to understand the trophic positions of host-associated fauna, and can aid in accurate constructions of food-webs on coral reefs. Scleractinian corals are hosts to hundreds of symbiont taxa, including fish and many invertebrate species. Some of these associated fauna are beneficial to their coral host(s), whereas other taxa can have detrimental effects, yet their impact is often difficult to determine. Coral-dwelling gall crabs (Cryptochiridae) are obligate, often host-specific, symbionts of scleractinian corals but the nature of this relationship is still under debate. Three Atlantic gall crab species (Kroppcarcinus siderastreicola, Opecarcinus hypostegus and Troglocarcinus corallicola) and their coral hosts’ tissue/mucus were collected from reefs in Guadeloupe. Stable carbon and nitrogen isotope values were measured for 57 crabs inhabiting host coral colonies belonging to seven different coral species (although only 27 colonies from five coral species were collected), alongside other potential food sources (epilithic algal matrix, plankton and particulate organic matter). The carbon and nitrogen isotope values of gall crabs relative to those of their respective coral host(s) and other possible food sources showed that coral tissue/mucus was the main food source for the crabs. The results of the mixing models further supported this finding, suggesting that corals are responsible for 40–70% of the crabs’ diet. In T. corallicola, the isotopic signature differed significantly between sexes, possibly caused by the high sexual dimorphism observed in this species. Here we showed that Atlantic gall crabs mainly dine on coral tissue and/or mucus excreted by their coral hosts, highlighting their nutritional dependence on their host. However, since coral mucus is continuously exuded by scleractinians, hence the energetic or metabolic drain for corals is expected to be minimal. Gall crabs depend on their coral hosts for settlement cues as larvae, for habitat as adults and - highlighted by this study - for food, essential for their subsistence. This obligate dependence on their hosts for all parts of their life makes them extremely vulnerable to reef degradation, and underlines the importance in understanding the exact nature of a relationship between symbiont and coral host.

共生动物与其宿主之间关系的确切性质往往并不清楚,但更多地了解这些错综复杂的生态相互作用对于了解宿主相关动物的营养位置至关重要,并有助于准确构建珊瑚礁上的食物网。硬骨鱼类珊瑚是数百种共生类群的宿主,包括鱼类和许多无脊椎动物。其中一些相关动物对其珊瑚宿主有益,而其他类群则可能产生有害影响,但它们的影响往往难以确定。珊瑚栖息的五倍子蟹(Cryptochiridae)是硬骨鱼类珊瑚的强制性共生生物,通常是特定宿主的共生生物,但这种关系的性质仍存在争议。研究人员从瓜德罗普岛的珊瑚礁中采集了三种大西洋五倍子蟹(Kroppcarcinus siderastreicola、Opecarcinus hypostegus 和 Troglocarcinus corallicola)及其珊瑚宿主的组织/粘液。对栖息在属于 7 个不同珊瑚物种的宿主珊瑚群中的 57 只螃蟹(尽管只收集到 5 个珊瑚物种的 27 个珊瑚群)的稳定碳和氮同位素值,以及其他潜在食物来源(附生藻类基质、浮游生物和颗粒有机物)进行了测量。五倍子蟹的碳和氮同位素值相对于其各自珊瑚宿主和其他可能食物来源的碳和氮同位素值显示,珊瑚组织/粘液是五倍子蟹的主要食物来源。混合模型的结果进一步支持了这一发现,表明珊瑚占螃蟹食物的 40-70%。在T. corallicola中,不同性别之间的同位素特征差异显著,这可能是由于在该物种中观察到的高度性二态性造成的。在这里,我们发现大西洋五倍子蟹主要以珊瑚组织和/或珊瑚宿主排泄的粘液为食,这突出表明了它们对宿主的营养依赖。然而,由于珊瑚粘液是由硬骨鱼类不断排出的,因此预计对珊瑚的能量或新陈代谢消耗很小。幼虫时期,胆蟹依赖珊瑚宿主提供定居线索;成虫时期,它们依赖珊瑚宿主提供栖息地;本研究强调,它们还依赖珊瑚宿主提供食物,这是它们赖以生存的必要条件。这种对宿主生命所有部分的强制性依赖使它们极易受到珊瑚礁退化的影响,并强调了了解共生体与珊瑚宿主之间关系的确切性质的重要性。
{"title":"Dining on corals: stable isotope evidence for close trophic connection between gall crabs (Cryptochiridae) and their stony coral hosts","authors":"Henrique Bravo, Charlotte R. Dromard, Marcel T. J. van der Meer, Anna Schleimer, Sancia E. T. van der Meij","doi":"10.1007/s13199-023-00968-y","DOIUrl":"https://doi.org/10.1007/s13199-023-00968-y","url":null,"abstract":"<p>The exact nature of the relationship between symbiont fauna and their hosts is often unclear, but knowing more about these intricate ecological interactions is vital to understand the trophic positions of host-associated fauna, and can aid in accurate constructions of food-webs on coral reefs. Scleractinian corals are hosts to hundreds of symbiont taxa, including fish and many invertebrate species. Some of these associated fauna are beneficial to their coral host(s), whereas other taxa can have detrimental effects, yet their impact is often difficult to determine. Coral-dwelling gall crabs (Cryptochiridae) are obligate, often host-specific, symbionts of scleractinian corals but the nature of this relationship is still under debate. Three Atlantic gall crab species (<i>Kroppcarcinus siderastreicola</i>, <i>Opecarcinus hypostegus</i> and <i>Troglocarcinus corallicola</i>) and their coral hosts’ tissue/mucus were collected from reefs in Guadeloupe. Stable carbon and nitrogen isotope values were measured for 57 crabs inhabiting host coral colonies belonging to seven different coral species (although only 27 colonies from five coral species were collected), alongside other potential food sources (epilithic algal matrix, plankton and particulate organic matter). The carbon and nitrogen isotope values of gall crabs relative to those of their respective coral host(s) and other possible food sources showed that coral tissue/mucus was the main food source for the crabs. The results of the mixing models further supported this finding, suggesting that corals are responsible for 40–70% of the crabs’ diet. In <i>T. corallicola</i>, the isotopic signature differed significantly between sexes, possibly caused by the high sexual dimorphism observed in this species. Here we showed that Atlantic gall crabs mainly dine on coral tissue and/or mucus excreted by their coral hosts, highlighting their nutritional dependence on their host. However, since coral mucus is continuously exuded by scleractinians, hence the energetic or metabolic drain for corals is expected to be minimal. Gall crabs depend on their coral hosts for settlement cues as larvae, for habitat as adults and - highlighted by this study - for food, essential for their subsistence. This obligate dependence on their hosts for all parts of their life makes them extremely vulnerable to reef degradation, and underlines the importance in understanding the exact nature of a relationship between symbiont and coral host.</p>","PeriodicalId":22123,"journal":{"name":"Symbiosis","volume":"213 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139508012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Symbiosis
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1