Pub Date : 2023-04-05DOI: 10.1142/s1682648523500014
B. Su, Fang-Ru Wu, Pin-Chen Liu
The severity of canine acute pancreatitis (AP) is highly variable. Clinically applicable severity scoring algorithms are limited. One-hundred-thirty-four dogs diagnosed with AP and hospitalized between 2009 and 2014 were selected for analysis to develop a severity scoring system. Eleven variables, i.e. age, hemoglobin, red blood cell count, albumin, total bilirubin, blood urea nitrogen, creatinine, phosphorus, potassium, presence of systemic inflammatory response syndrome and oliguria/anuria after rehydration (Day 2 to Day 4) were significantly abnormal and selected by logistic regression to predict the outcome. A scoring system was developed by incorporating weighting factors assigned to each quartile based on the odds ratio integer value. A predictive score is the sum of all weighting factors for each dog. Receiver-operating curve analyses were performed to assess the sensitivity, specificity and optimal cut-off point of the outcome predictions. The scores of 134 dogs after rehydration ranged from 11 to 78 points. The mean score was [Formula: see text], and the median score was 23 points. The optimal cut-off point for outcome prediction of the scoring system was 35.5, with a sensitivity of 96% and a specificity of 89.3%. The mortality was 85.5% with a score of [Formula: see text], whereas 3.8% had a score of [Formula: see text]. Dogs with a score [Formula: see text] all survived. The severity scoring system provides a reliable and clinically applicable method to assess disease severity in dogs with clinically AP at the time point after rehydration.
{"title":"A DISEASE SEVERITY SCORING SYSTEM IN DOGS WITH CLINICALLY ACUTE PANCREATITIS","authors":"B. Su, Fang-Ru Wu, Pin-Chen Liu","doi":"10.1142/s1682648523500014","DOIUrl":"https://doi.org/10.1142/s1682648523500014","url":null,"abstract":"The severity of canine acute pancreatitis (AP) is highly variable. Clinically applicable severity scoring algorithms are limited. One-hundred-thirty-four dogs diagnosed with AP and hospitalized between 2009 and 2014 were selected for analysis to develop a severity scoring system. Eleven variables, i.e. age, hemoglobin, red blood cell count, albumin, total bilirubin, blood urea nitrogen, creatinine, phosphorus, potassium, presence of systemic inflammatory response syndrome and oliguria/anuria after rehydration (Day 2 to Day 4) were significantly abnormal and selected by logistic regression to predict the outcome. A scoring system was developed by incorporating weighting factors assigned to each quartile based on the odds ratio integer value. A predictive score is the sum of all weighting factors for each dog. Receiver-operating curve analyses were performed to assess the sensitivity, specificity and optimal cut-off point of the outcome predictions. The scores of 134 dogs after rehydration ranged from 11 to 78 points. The mean score was [Formula: see text], and the median score was 23 points. The optimal cut-off point for outcome prediction of the scoring system was 35.5, with a sensitivity of 96% and a specificity of 89.3%. The mortality was 85.5% with a score of [Formula: see text], whereas 3.8% had a score of [Formula: see text]. Dogs with a score [Formula: see text] all survived. The severity scoring system provides a reliable and clinically applicable method to assess disease severity in dogs with clinically AP at the time point after rehydration.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76303610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-05DOI: 10.1142/s1682648523720022
Hung-Hsien Hsiao, Pin-Chen Liu
An 8-month-old intact female shorthair domestic cat was referred to the National Chung Hsing University Veterinary Medical Teaching Hospital for uncontrolled chronic otitis in both ears. The affected ears revealed purulent discharge, erosion with bleeding and brownish-black proliferative tissue in the opening of both ear canals. Cytological examination of the discharge revealed the presence of gram-positive coccoid bacteria. We carried out histopathological examinations of biopsies obtained from friable and proliferative tissues of both ears. Based on the clinical and histopathological characteristics, proliferative and necrotizing otitis externa (PNOE) was diagnosed. Treatment was initiated with a combination of twice-daily application of 0.1% tacrolimus ointment and a once-daily otic lotion containing hydrocortisone aceponate, miconazole nitrate and gentamicin sulfate following the administration of once-daily anti-bacterial flush which contains tromethamine, edetate disodium dehydrate (EDTA) buffered to pH 8 with tromethamine hydrochloride and deionized water. The antibiotic ear lotion was withdrawn in the absence of purulent discharge in both ears after 11 weeks. Most proliferative lesions resolved after 17 weeks, and we tapered the dosage of 0.1% tacrolimus from once daily to once weekly during the year follow-up period. However, in our case, partial remission of PNOE was observed with the application of a combination of 0.1% tacrolimus ointment and a commercial ear lotion. The cat was healthy and had a good quality of life. To our knowledge, this is the first report of PNOE in a cat in Taiwan.
{"title":"CASE REPORT: PROLIFERATIVE AND NECROTIZING OTITIS EXTERNA IN A CAT","authors":"Hung-Hsien Hsiao, Pin-Chen Liu","doi":"10.1142/s1682648523720022","DOIUrl":"https://doi.org/10.1142/s1682648523720022","url":null,"abstract":"An 8-month-old intact female shorthair domestic cat was referred to the National Chung Hsing University Veterinary Medical Teaching Hospital for uncontrolled chronic otitis in both ears. The affected ears revealed purulent discharge, erosion with bleeding and brownish-black proliferative tissue in the opening of both ear canals. Cytological examination of the discharge revealed the presence of gram-positive coccoid bacteria. We carried out histopathological examinations of biopsies obtained from friable and proliferative tissues of both ears. Based on the clinical and histopathological characteristics, proliferative and necrotizing otitis externa (PNOE) was diagnosed. Treatment was initiated with a combination of twice-daily application of 0.1% tacrolimus ointment and a once-daily otic lotion containing hydrocortisone aceponate, miconazole nitrate and gentamicin sulfate following the administration of once-daily anti-bacterial flush which contains tromethamine, edetate disodium dehydrate (EDTA) buffered to pH 8 with tromethamine hydrochloride and deionized water. The antibiotic ear lotion was withdrawn in the absence of purulent discharge in both ears after 11 weeks. Most proliferative lesions resolved after 17 weeks, and we tapered the dosage of 0.1% tacrolimus from once daily to once weekly during the year follow-up period. However, in our case, partial remission of PNOE was observed with the application of a combination of 0.1% tacrolimus ointment and a commercial ear lotion. The cat was healthy and had a good quality of life. To our knowledge, this is the first report of PNOE in a cat in Taiwan.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"159 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85354089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-01DOI: 10.1142/s1682648523500026
Dieu-An Le Nguyen, Li-Wu Cheng, Pei-Chi Wang, Shih‐Chu Chen
Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009. Recently, it has been identified as an emerging threat to shrimp aquaculture industries due to the significant economic losses. The causative agent of AHPND is Vibrio parahaemolyticus (VPAHPND). This study aimed to screen AHPND toxins in bacterial isolates from farmed Penaeus vannamei in Taiwan. In Southern Taiwan, sixty shrimp cases were collected. The prevalence of AHPND positive cases was 65% (39/60), with 4 severely infected cases and 35 moderately infected ones. Eighty-nine bacterial strains were also isolated from white-leg shrimp farms. All of the bacterial isolates were gram-negative, short-rod shaped, and motility-positive, with catalase, oxidase, indole, and glucose fermentation. Eighty-nine strains were identified as V. parahaemolyticus using polymerase chain reaction (PCR). Fifty-nine V. parahaemolyticus isolates tested positive for toxin genes (PirA and PirB) using nested PCR, among them four isolates showed positive in the first step nest PCR. Phylogenetic analysis of these four AHPND isolates revealed complete similarity with AHPND isolates from China, Malaysia, and Thailand. The challenge test showed that these strains produced mass death of up to 90–100% within 24 h after receiving 107 CFU/shrimp intramuscular injection. Histopathological examination of the hepatopancreas revealed haemocyte infiltration and the presence of bacteria within hepatopancreatic lumen. Our findings show that V. parahaemolyticus is the causal agent of AHPND in Taiwanese shrimp farms.
{"title":"PHENOTYPIC, PHYLOGENETIC ANALYSIS AND PATHOGENIC DETECTION OF VIBRIO PARAHAEMOLYTICUS IN FARMED PENAEUS VANNAMEI IN TAIWAN","authors":"Dieu-An Le Nguyen, Li-Wu Cheng, Pei-Chi Wang, Shih‐Chu Chen","doi":"10.1142/s1682648523500026","DOIUrl":"https://doi.org/10.1142/s1682648523500026","url":null,"abstract":"Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009. Recently, it has been identified as an emerging threat to shrimp aquaculture industries due to the significant economic losses. The causative agent of AHPND is Vibrio parahaemolyticus (VPAHPND). This study aimed to screen AHPND toxins in bacterial isolates from farmed Penaeus vannamei in Taiwan. In Southern Taiwan, sixty shrimp cases were collected. The prevalence of AHPND positive cases was 65% (39/60), with 4 severely infected cases and 35 moderately infected ones. Eighty-nine bacterial strains were also isolated from white-leg shrimp farms. All of the bacterial isolates were gram-negative, short-rod shaped, and motility-positive, with catalase, oxidase, indole, and glucose fermentation. Eighty-nine strains were identified as V. parahaemolyticus using polymerase chain reaction (PCR). Fifty-nine V. parahaemolyticus isolates tested positive for toxin genes (PirA and PirB) using nested PCR, among them four isolates showed positive in the first step nest PCR. Phylogenetic analysis of these four AHPND isolates revealed complete similarity with AHPND isolates from China, Malaysia, and Thailand. The challenge test showed that these strains produced mass death of up to 90–100% within 24 h after receiving 107 CFU/shrimp intramuscular injection. Histopathological examination of the hepatopancreas revealed haemocyte infiltration and the presence of bacteria within hepatopancreatic lumen. Our findings show that V. parahaemolyticus is the causal agent of AHPND in Taiwanese shrimp farms.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83134898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-18DOI: 10.1142/s1682648522720039
Huan-Yu Hsu, N. Thongrueang, Guan‐Ming Ke, Hsu-Hsun Lee
Over-mothering syndrome is common in intensive deer farming; the wound is usually deep and hard to take care of, and results in neonatal mortality increased by bacterial infection related to the wound. Regenerate therapy might be an option for treating severe wounds due to over-mothering syndrome. Autologous platelet-rich plasma (PRP) is a widely used method to treat orthopedic issues in humans and animals. It contains several concentrated growth factors to promote tissue regeneration, without the complications of overreaction or of rejection issues. This report presents the successful treatment of autologous PRP in a fawn with a [Formula: see text][Formula: see text]cm wound in the perineal area, caused by an over-mothering doe. Autologous PRP was injected on both sides of the proximal of the gluteobiceps muscle and applied 3 times over the 20-day treatment period. The wound displayed rapid tissue regeneration with no complications. This successful application of PRP is the first report to enhance the regeneration of the vulva and the anus in a large animal. The PRP was injected on the proximal of the wound rather than directly on the wound to reduce potential irritation from physical contact to the wound, and the regeneration process was nice and fast. This protocol could provide clinical veterinarians with an effective and low labor cost method to treat severe and contaminated wounds in the perineal area.
{"title":"A SUCCESSFUL PLATELET-RICH PLASMA TREATMENT IN A FAWN WITH OVER-MOTHERING WOUND IN THE PERINEAL AREA","authors":"Huan-Yu Hsu, N. Thongrueang, Guan‐Ming Ke, Hsu-Hsun Lee","doi":"10.1142/s1682648522720039","DOIUrl":"https://doi.org/10.1142/s1682648522720039","url":null,"abstract":"Over-mothering syndrome is common in intensive deer farming; the wound is usually deep and hard to take care of, and results in neonatal mortality increased by bacterial infection related to the wound. Regenerate therapy might be an option for treating severe wounds due to over-mothering syndrome. Autologous platelet-rich plasma (PRP) is a widely used method to treat orthopedic issues in humans and animals. It contains several concentrated growth factors to promote tissue regeneration, without the complications of overreaction or of rejection issues. This report presents the successful treatment of autologous PRP in a fawn with a [Formula: see text][Formula: see text]cm wound in the perineal area, caused by an over-mothering doe. Autologous PRP was injected on both sides of the proximal of the gluteobiceps muscle and applied 3 times over the 20-day treatment period. The wound displayed rapid tissue regeneration with no complications. This successful application of PRP is the first report to enhance the regeneration of the vulva and the anus in a large animal. The PRP was injected on the proximal of the wound rather than directly on the wound to reduce potential irritation from physical contact to the wound, and the regeneration process was nice and fast. This protocol could provide clinical veterinarians with an effective and low labor cost method to treat severe and contaminated wounds in the perineal area.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"183 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74627066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-31DOI: 10.1142/s1682648522500068
Chishih Chu, Changyou Yu, Ting-Chun Kuo
Poultry and its products are often referred to as reservoirs of Salmonella to infect humans. Salmonella serovars were determined from samples of terminated eggs (early-terminated eggs, EEs, and late-terminated eggs, LEs) on farms YA (layers), YB (native hybrid broilers, NHCs), and YF (NHCs); one-day-old chicks (OCs) on farm YB; and shoe covers (SCs) on farms YB, YC (broilers), YD (broilers), YE (NHCs), and YF using multiplex PCR and agglutination methods. One hundred isolates were examined for susceptibility to 13 antimicrobials and profiles of pulsotypes and plasmids. Nine serovars were identified as serogroup B Agona and Schwarzengrund, serogroup C1 Montevideo and Mbandaka, serogroup C2 Albany, Kentucky and Newport, serogroup E Weltevreden, and serogroup G Kedougou. Different from those in 2003, Albany, Kentucky, Montevideo, and Mbandaka were the predominant serovars that differed in chromosomal and plasmid variations and antibiogram profiles. Albany and Kentucky isolates had higher resistance levels to the antimicrobials than the Montevideo and Mbandaka isolates. The antibiogram differences among the serovars may be related to the presence of antibiotic resistance genes in Salmonella genomic island 1-F (SGI1-F) and plasmids in Albany isolates. Albany and Kentucky exhibited the largest chromosomal and plasmid variations, respectively. The main serovars associated with embryogenesis termination were Mbandaka and Montevideo in EEs and LEs, Newport in NHCs, and Kedougou in LEs from layers. Furthermore, Montevideo and Agona were the only serovars detected in OCs, and Albany and Kentucky were the main serovars on SCs. Farm management also affected the Salmonella prevalence and predominant serovars.
{"title":"CHARACTERIZATION OF FOUR MAIN SALMONELLA SEROVARS, ALBANY, KENTUCKY, MBANDAKA, AND MONTEVIDEO, ASSOCIATED WITH CHICKEN LINES, FARMS, AND SAMPLE SOURCES IN SOUTHERN TAIWAN","authors":"Chishih Chu, Changyou Yu, Ting-Chun Kuo","doi":"10.1142/s1682648522500068","DOIUrl":"https://doi.org/10.1142/s1682648522500068","url":null,"abstract":"Poultry and its products are often referred to as reservoirs of Salmonella to infect humans. Salmonella serovars were determined from samples of terminated eggs (early-terminated eggs, EEs, and late-terminated eggs, LEs) on farms YA (layers), YB (native hybrid broilers, NHCs), and YF (NHCs); one-day-old chicks (OCs) on farm YB; and shoe covers (SCs) on farms YB, YC (broilers), YD (broilers), YE (NHCs), and YF using multiplex PCR and agglutination methods. One hundred isolates were examined for susceptibility to 13 antimicrobials and profiles of pulsotypes and plasmids. Nine serovars were identified as serogroup B Agona and Schwarzengrund, serogroup C1 Montevideo and Mbandaka, serogroup C2 Albany, Kentucky and Newport, serogroup E Weltevreden, and serogroup G Kedougou. Different from those in 2003, Albany, Kentucky, Montevideo, and Mbandaka were the predominant serovars that differed in chromosomal and plasmid variations and antibiogram profiles. Albany and Kentucky isolates had higher resistance levels to the antimicrobials than the Montevideo and Mbandaka isolates. The antibiogram differences among the serovars may be related to the presence of antibiotic resistance genes in Salmonella genomic island 1-F (SGI1-F) and plasmids in Albany isolates. Albany and Kentucky exhibited the largest chromosomal and plasmid variations, respectively. The main serovars associated with embryogenesis termination were Mbandaka and Montevideo in EEs and LEs, Newport in NHCs, and Kedougou in LEs from layers. Furthermore, Montevideo and Agona were the only serovars detected in OCs, and Albany and Kentucky were the main serovars on SCs. Farm management also affected the Salmonella prevalence and predominant serovars.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"117 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91423331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-22DOI: 10.1142/s1682648522720027
Shu-Fang Yang, Heng-Leng Yang, Peng-Chen Wang, S. Liang
Two adult poodles were referred for severe corneal ulcers in both eyes (oculi uterque [OU]) for 1.5 months, and swollen eyelids for approximately 1 week. Ophthalmic examination revealed diffuse corneal edema with grayish deposit and ulcer, uveitis, glaucoma, grayish deposit and necrosis on the third eyelid and conjunctiva, eyelids necrosis, eyelid-eyeball adhesions, and blindness. Cytological examination of the conjunctiva and cornea revealed neutrophilic inflammation, and a bacterial culture revealed Staphylococcus aureus. An adrenocorticotropic hormone stimulation test was performed, strongly indicating iatrogenic hyperadrenocorticism. No improvement was observed after 2 weeks of antibiotic treatment. Fungal cultures from the cornea and conjunctiva were positive for Candida spp. Specific antifungal treatment included topical 5% natamycin eye drops (OU six times daily) and oral itraconazole (5[Formula: see text]mg/kg q12[Formula: see text]h). The grayish deposits of the conjunctiva and cornea were sloughed off, thus facilitating the healing of corneal and eyelids ulcers, over the next 3–4 weeks. Phthisis bulbi and eyelid-eyeball adhesions developed in both dogs after 6 months of follow-up. Based on the fungal isolation by culture, clinical signs, and response to antifungal treatment, Candida spp. panophthalmitis was diagnosed. Fungi should be considered a potential organism in panophthalmitis, especially with a medical history of steroids and antibiotic use. This case report demonstrates the grayish deposits of the cornea, conjunctiva, and third eyelid as possible characteristics of Candida infection in the dogs. To our knowledge, this is the first report of Candida panophthalmitis with iatrogenic hyperadrenocorticism in dogs.
{"title":"CANDIDA PANOPHTHALMITIS WITH IATROGENIC HYPERADRENOCORTICISM IN TWO DOGS","authors":"Shu-Fang Yang, Heng-Leng Yang, Peng-Chen Wang, S. Liang","doi":"10.1142/s1682648522720027","DOIUrl":"https://doi.org/10.1142/s1682648522720027","url":null,"abstract":"Two adult poodles were referred for severe corneal ulcers in both eyes (oculi uterque [OU]) for 1.5 months, and swollen eyelids for approximately 1 week. Ophthalmic examination revealed diffuse corneal edema with grayish deposit and ulcer, uveitis, glaucoma, grayish deposit and necrosis on the third eyelid and conjunctiva, eyelids necrosis, eyelid-eyeball adhesions, and blindness. Cytological examination of the conjunctiva and cornea revealed neutrophilic inflammation, and a bacterial culture revealed Staphylococcus aureus. An adrenocorticotropic hormone stimulation test was performed, strongly indicating iatrogenic hyperadrenocorticism. No improvement was observed after 2 weeks of antibiotic treatment. Fungal cultures from the cornea and conjunctiva were positive for Candida spp. Specific antifungal treatment included topical 5% natamycin eye drops (OU six times daily) and oral itraconazole (5[Formula: see text]mg/kg q12[Formula: see text]h). The grayish deposits of the conjunctiva and cornea were sloughed off, thus facilitating the healing of corneal and eyelids ulcers, over the next 3–4 weeks. Phthisis bulbi and eyelid-eyeball adhesions developed in both dogs after 6 months of follow-up. Based on the fungal isolation by culture, clinical signs, and response to antifungal treatment, Candida spp. panophthalmitis was diagnosed. Fungi should be considered a potential organism in panophthalmitis, especially with a medical history of steroids and antibiotic use. This case report demonstrates the grayish deposits of the cornea, conjunctiva, and third eyelid as possible characteristics of Candida infection in the dogs. To our knowledge, this is the first report of Candida panophthalmitis with iatrogenic hyperadrenocorticism in dogs.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85577138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-12DOI: 10.1142/s1682648522500032
D. Lo, Tsung-Li Yeh, H. Kuo, Ching-Fen Wu
Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (AP) infection leads to considerable financial loss in the swine industry worldwide. Multilocus sequence typing (MLST), a definitive molecular typing technique that is highly reproducible and is capable of comparing data across laboratories, has not been studied on AP. Therefore, the aim of this work is to develop a MLST assay to characterize AP isolates collected from Taiwan. A total of 85 AP isolates collected from pleuropneumonia of diseased pigs with respiratory symptoms and seven reference isolates from other countries were included for comparison. Seven housekeeping genes (recF, gly, rho, tpi, pyk, recN, and rpo) were selected for sequencing. Capsule types that determine serotypes and Apx toxins were analyzed by the polymerase chain reaction (PCR). The sequencing results showed that the 7 housekeeping genes differentiated the 92 isolates into 14 sequence types that belonged to three major clonal complexes and five singletons by eBURST. The 85 isolates assigned to detection of capsule types and Apx toxins showed that serotype 1 with Apx I, II, IV (55.3%) followed by serotype 5 with Apx I, II, IV (29.4%) were the most prevalent in Taiwan. Notably, serotype 15 was identified for the first time. Clonal complexes based on ST profiles from MLST analysis were highly associated with the distribution of capsule types, suggesting that MLST scheme was sufficient to identify DNA samples directly from AP. Therefore, MLST might be a useful tool for identification and further epidemiological assay of AP.
{"title":"Development of Multilocus Sequence Typing (MLST) of Actinobacillus Pleuropneumniae","authors":"D. Lo, Tsung-Li Yeh, H. Kuo, Ching-Fen Wu","doi":"10.1142/s1682648522500032","DOIUrl":"https://doi.org/10.1142/s1682648522500032","url":null,"abstract":"Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (AP) infection leads to considerable financial loss in the swine industry worldwide. Multilocus sequence typing (MLST), a definitive molecular typing technique that is highly reproducible and is capable of comparing data across laboratories, has not been studied on AP. Therefore, the aim of this work is to develop a MLST assay to characterize AP isolates collected from Taiwan. A total of 85 AP isolates collected from pleuropneumonia of diseased pigs with respiratory symptoms and seven reference isolates from other countries were included for comparison. Seven housekeeping genes (recF, gly, rho, tpi, pyk, recN, and rpo) were selected for sequencing. Capsule types that determine serotypes and Apx toxins were analyzed by the polymerase chain reaction (PCR). The sequencing results showed that the 7 housekeeping genes differentiated the 92 isolates into 14 sequence types that belonged to three major clonal complexes and five singletons by eBURST. The 85 isolates assigned to detection of capsule types and Apx toxins showed that serotype 1 with Apx I, II, IV (55.3%) followed by serotype 5 with Apx I, II, IV (29.4%) were the most prevalent in Taiwan. Notably, serotype 15 was identified for the first time. Clonal complexes based on ST profiles from MLST analysis were highly associated with the distribution of capsule types, suggesting that MLST scheme was sufficient to identify DNA samples directly from AP. Therefore, MLST might be a useful tool for identification and further epidemiological assay of AP.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91075599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-12DOI: 10.1142/s1682648522720015
D. Lo, H. Kuo, Ning-Chieh Twu, Ching-Fen Wu
This case report describes New Neonatal Porcine Diarrhea Syndrome (NNPDS) caused by Enterococcus hirae. Four piglets at 3–5 days old with diarrhea were submitted for diagnostic investigation. The histopathological analysis revealed numerous large Gram-positive cocci adherent to the luminal surface from the tip to the base of the villi. The organisms were tightly adherent to enterocytes on the villous tips and exfoliated enterocytes within the luminal detritus. In situ hybridization revealed positive signals of E. hirae, the enteroadherent Gram-positive cocci, in the intestinal tissue. The swab of distal jejunum and gut content was cultured positive for E. hirae. Taken all results together, it was concluded that neonatal diarrhea in piglets was caused by E. hirae.
{"title":"CASE REPORT: ENTEROCOCCUS HIRAE INFECTION IN SUCKLING PIGS","authors":"D. Lo, H. Kuo, Ning-Chieh Twu, Ching-Fen Wu","doi":"10.1142/s1682648522720015","DOIUrl":"https://doi.org/10.1142/s1682648522720015","url":null,"abstract":"This case report describes New Neonatal Porcine Diarrhea Syndrome (NNPDS) caused by Enterococcus hirae. Four piglets at 3–5 days old with diarrhea were submitted for diagnostic investigation. The histopathological analysis revealed numerous large Gram-positive cocci adherent to the luminal surface from the tip to the base of the villi. The organisms were tightly adherent to enterocytes on the villous tips and exfoliated enterocytes within the luminal detritus. In situ hybridization revealed positive signals of E. hirae, the enteroadherent Gram-positive cocci, in the intestinal tissue. The swab of distal jejunum and gut content was cultured positive for E. hirae. Taken all results together, it was concluded that neonatal diarrhea in piglets was caused by E. hirae.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"305 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77218676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-09DOI: 10.1142/s1682648522500044
Yong-Mi Pyon, Hyok Ryo, Sun-Nam Pak, Il-Ryong Ko
The hepatocytes were isolated from the liver of different week-age of healthy rabbits by a single two-stage pass perfusion method and suspended in Hanks’ balanced salt solution (Ca[Formula: see text]-, Mg[Formula: see text]-free). The field strains of RHDV were inoculated into the suspension and incubated for 2 days at 37∘C. After incubation, the HA titer of each strain of RHDV in supernatant of virus growth was measured using human erythrocyte O type. There were differences in the HA titers of RHDV strains according to the hepatocyte of different week-age of rabbits, the number of hepatocyte, the strain of virus (RHDV-1, RHDV-2, RHDV-3) and the duration of incubation. The HA titer was highest when the virus was cultured in the hepatocytes isolated from more than 12 week-aged rabbits. When non-immune rabbits were inoculated with the strains of virus (RHDV-1, RHDV-2, RHDV-3). respectively, all rabbits (3 heads of rabbit per group) infected with RHDV-1 and RHDV-3 died of the disease within 48[Formula: see text]h, while only one rabbit infected with RHDV-2 died within 72[Formula: see text]h. When immunized rabbits by inactivated virus strains (RHDV-1, RHDV-2 and RHDV-3), 2 strains, RHDV-1 and RHDV-3, showed the same level of HI titers with inactivated homogenate, but RHDV-2 did lower the level of titer than the homogenate.
{"title":"PATHOGENICITY AND IMMUNOGENICITY FOR SOME RABBIT HEMORRHAGIC DISEASE VIRUS(RHDV) STRAINS PROPAGATED IN ISOLATED HEPATOCYTES OF RABBIT","authors":"Yong-Mi Pyon, Hyok Ryo, Sun-Nam Pak, Il-Ryong Ko","doi":"10.1142/s1682648522500044","DOIUrl":"https://doi.org/10.1142/s1682648522500044","url":null,"abstract":"The hepatocytes were isolated from the liver of different week-age of healthy rabbits by a single two-stage pass perfusion method and suspended in Hanks’ balanced salt solution (Ca[Formula: see text]-, Mg[Formula: see text]-free). The field strains of RHDV were inoculated into the suspension and incubated for 2 days at 37∘C. After incubation, the HA titer of each strain of RHDV in supernatant of virus growth was measured using human erythrocyte O type. There were differences in the HA titers of RHDV strains according to the hepatocyte of different week-age of rabbits, the number of hepatocyte, the strain of virus (RHDV-1, RHDV-2, RHDV-3) and the duration of incubation. The HA titer was highest when the virus was cultured in the hepatocytes isolated from more than 12 week-aged rabbits. When non-immune rabbits were inoculated with the strains of virus (RHDV-1, RHDV-2, RHDV-3). respectively, all rabbits (3 heads of rabbit per group) infected with RHDV-1 and RHDV-3 died of the disease within 48[Formula: see text]h, while only one rabbit infected with RHDV-2 died within 72[Formula: see text]h. When immunized rabbits by inactivated virus strains (RHDV-1, RHDV-2 and RHDV-3), 2 strains, RHDV-1 and RHDV-3, showed the same level of HI titers with inactivated homogenate, but RHDV-2 did lower the level of titer than the homogenate.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"9 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91424821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-16DOI: 10.1142/s1682648522500020
Chi-Hsuan Sung, Pin-Chen Liu, Julia Chu-Ning Hsu, C. Chou
C-reactive protein (CRP) is an acute phase protein that may increase in response to various inflammatory conditions or infections in human and animals. It has been suggestive to use CRP levels to diagnose, monitor and prognose canine inflammatory diseases; however, its practical uses remain obscure. We have comparatively demonstrated the use of CRP, total white blood cell (WBC) counts and neutrophil counts to monitor the progression of four canine common disorders, including acute pancreatitis, acute abdomen syndrome, neoplastic diseases and pyometra. A total of 52 cases were included in this study. The CRP concentration, complete blood count and protein profile were measured before and on days 1, 2 of hospitalization, day of discharge and the day of follow-up visit. Statistical analyses indicated that CRP is the only parameter with elevated concentrations significantly differ among different days. None of the clinicopathological variables correlated well with serum CRP concentrations except for visual negative correlation to albumin. Differential efficiencies were observed when using CRP and total WBC counts to monitor the four disorders. The CRP and total WBC counts were equally efficient in monitoring pyometra and neoplastic disease patients, while in acute pancreatitis and acute abdomen syndrome, the use of CRP was clearly advantageous. Overall, CRP is a more sensitive marker showing an evident up-and-down trend and with greater degree of responses than total WBC and neutrophil counts, which enable CRP a better quantitative analysis of disease progression. In conclusion, CRP levels exhibit various degrees of clinical potential to monitor the development and treatment progress of canine diseases with inflammatory components. The inclusion of other inflammatory biomarkers and the study of CRP isoforms warrant further investigation.
{"title":"C-REACTIVE PROTEIN AS AN EFFICIENT INDICATOR MONITORING AND PROGNOSING CANINE INFLAMMATORY DISEASES","authors":"Chi-Hsuan Sung, Pin-Chen Liu, Julia Chu-Ning Hsu, C. Chou","doi":"10.1142/s1682648522500020","DOIUrl":"https://doi.org/10.1142/s1682648522500020","url":null,"abstract":"C-reactive protein (CRP) is an acute phase protein that may increase in response to various inflammatory conditions or infections in human and animals. It has been suggestive to use CRP levels to diagnose, monitor and prognose canine inflammatory diseases; however, its practical uses remain obscure. We have comparatively demonstrated the use of CRP, total white blood cell (WBC) counts and neutrophil counts to monitor the progression of four canine common disorders, including acute pancreatitis, acute abdomen syndrome, neoplastic diseases and pyometra. A total of 52 cases were included in this study. The CRP concentration, complete blood count and protein profile were measured before and on days 1, 2 of hospitalization, day of discharge and the day of follow-up visit. Statistical analyses indicated that CRP is the only parameter with elevated concentrations significantly differ among different days. None of the clinicopathological variables correlated well with serum CRP concentrations except for visual negative correlation to albumin. Differential efficiencies were observed when using CRP and total WBC counts to monitor the four disorders. The CRP and total WBC counts were equally efficient in monitoring pyometra and neoplastic disease patients, while in acute pancreatitis and acute abdomen syndrome, the use of CRP was clearly advantageous. Overall, CRP is a more sensitive marker showing an evident up-and-down trend and with greater degree of responses than total WBC and neutrophil counts, which enable CRP a better quantitative analysis of disease progression. In conclusion, CRP levels exhibit various degrees of clinical potential to monitor the development and treatment progress of canine diseases with inflammatory components. The inclusion of other inflammatory biomarkers and the study of CRP isoforms warrant further investigation.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85377326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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