Zhongying Liu, Qiansong Ran, Congjun Yuan, Shimao Fang, Ke Pan, Lin Long
To investigate the differences in taste characteristics and antioxidant properties of four types of tea substitutes. After sensory review and electronic tongue indicated that the taste characteristics of the four substitute teas was sweetness, bitterness and astringency. There were a total of 136 flavonoid metabolites in 12 categories and 12 sugar metabolites were identified in the four substitute teas with ultra-performance liquid chromatography tandem-mass spectrometry metabolomics targeted detection. Flavonoids were significantly positively correlated with antioxidant properties, among them, 15 were positively associated with clearing ABTS radicals and 5 were positively associated with clearing DPPH radicals, and sugars were significantly negatively correlated with antioxidant properties. The antioxidant properties were in the following order: TC > KD > LY > QQ. Conclusion: Significant differences in the content of flavonoids and sugar metabolites are the main reasons for the formation of the flavour characteristics and antioxidant differences of the four substitute teas.
{"title":"Differences in taste characteristics and antioxidant properties of four substitute teas based on a targeted metabolomics approach","authors":"Zhongying Liu, Qiansong Ran, Congjun Yuan, Shimao Fang, Ke Pan, Lin Long","doi":"10.48130/bpr-2023-0033","DOIUrl":"https://doi.org/10.48130/bpr-2023-0033","url":null,"abstract":"To investigate the differences in taste characteristics and antioxidant properties of four types of tea substitutes. After sensory review and electronic tongue indicated that the taste characteristics of the four substitute teas was sweetness, bitterness and astringency. There were a total of 136 flavonoid metabolites in 12 categories and 12 sugar metabolites were identified in the four substitute teas with ultra-performance liquid chromatography tandem-mass spectrometry metabolomics targeted detection. Flavonoids were significantly positively correlated with antioxidant properties, among them, 15 were positively associated with clearing ABTS radicals and 5 were positively associated with clearing DPPH radicals, and sugars were significantly negatively correlated with antioxidant properties. The antioxidant properties were in the following order: TC > KD > LY > QQ. Conclusion: Significant differences in the content of flavonoids and sugar metabolites are the main reasons for the formation of the flavour characteristics and antioxidant differences of the four substitute teas.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135212485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paul Musa Lahai, Mohamed Alieu Bah, Mohamed Tailu Lahai, Peter Osobase Aikpokpodion, Raymonda Adeline Bernadette Johnson
Global coffee production is dominated by Coffea arabica (Arabica coffee) and C. canephora (Robusta coffee) due to their relatively high-yielding and quality attributes as opposed to other coffee species. Despite these advantages, production of Arabica and Robusta coffee is facing mounting challenges though not limited to increasing prevalence and severity of biotic and abiotic stresses. These challenges bring forth an indication that the global coffee crop portfolio requires diversification to ensure resilience to the key challenges for sustainable production.Sierra Leone is in the center of genetic diversity of genus Coffea, and the countryhosts rich coffee genetic resources. The C. stenophylla, C. affinis and possibly other wild relative species are indigenous to Sierra Leone and these species offer great potential for a new coffee market and income generation. However, moreefforts of conservation and genetic improvement on these species, are needed to realize these opportunities.The objective of this paper is to review the coffee genetic resources in Sierra Leone with an emphasis on the wild coffee species including their conservation status, and the phenotypic and molecular characterization. We also presented perspectives for future genetic improvement of C. stenophylla, and discussed breeding methods,combiningability, and molecular marker-assisted prediction of hybrid vigor. Moreover, with the availability of recently developed single nucleotide polymorphisms (SNP) markers on C. stenophylla, we suggest that new technologies of molecular breeding, such as genomic selection can significantly accelerate the breeding progress and deliver improved varieties with high yield, good adaptability, and disease resistance.
{"title":"Genetic diversity of coffee germplasm in Sierra Leone: implications for conservation and breeding programs","authors":"Paul Musa Lahai, Mohamed Alieu Bah, Mohamed Tailu Lahai, Peter Osobase Aikpokpodion, Raymonda Adeline Bernadette Johnson","doi":"10.48130/bpr-2023-0026","DOIUrl":"https://doi.org/10.48130/bpr-2023-0026","url":null,"abstract":"Global coffee production is dominated by <italic>Coffea arabica</italic> (Arabica coffee) and <italic>C. canephora </italic>(Robusta coffee) due to their relatively high-yielding and quality attributes as opposed to other coffee species. Despite these advantages, production of Arabica and Robusta coffee is facing mounting challenges though not limited to increasing prevalence and severity of biotic and abiotic stresses. These challenges bring forth an indication that the global coffee crop portfolio requires diversification to ensure resilience to the key challenges for sustainable production.Sierra Leone is in the center of genetic diversity of genus <italic>Coffea,</italic> and the countryhosts rich coffee genetic resources. The <italic>C. stenophylla</italic>, <italic>C. affinis</italic> and possibly other wild relative species are indigenous to Sierra Leone and these species offer great potential for a new coffee market and income generation. However, moreefforts of conservation and genetic improvement on these species, are needed to realize these opportunities.The objective of this paper is to review the coffee genetic resources in Sierra Leone with an emphasis on the wild coffee species including their conservation status, and the phenotypic and molecular characterization. We also presented perspectives for future genetic improvement of <italic>C. stenophylla</italic>, and discussed breeding methods,combiningability, and molecular marker-assisted prediction of hybrid vigor. Moreover, with the availability of recently developed single nucleotide polymorphisms (SNP) markers on <italic>C. stenophylla</italic>, we suggest that new technologies of molecular breeding, such as genomic selection can significantly accelerate the breeding progress and deliver improved varieties with high yield, good adaptability, and disease resistance.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135699033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jianjian Gao, Dan Chen, Zhiyuan Lin, Jiakun Peng, Shuai Yu, Chuang Zhou, Huimin Jiang, Ruofan Sun, Zhi Lin, Weidong Dai
Diabetes mellitus (DM) is a pressing global public health issue with a high incidence of morbidity and mortality due to its complications. Although there are many medicines available for the treatment of DM, long-term use causes various adverse effects, such as diarrhea, vomiting, and nausea. Tea, owing to its richness of diverse bioactive components including tea polyphenols, tea polysaccharides, and alkaloids, has displayed promising antidiabetic properties. Screening antidiabetic bioactive compounds derived from teas is receiving increasing attention. Epidemiological and clinical investigations have demonstrated an inverse relationship between tea consumption and the incidence of DM. Both in vitro and in vivo experiments have substantiated the hypoglycemic effects of tea and its bioactive components through several possible mechanisms, including improvement of insulin resistance, inhibition of carbohydrates digestion and absorption (inhibit α-amylase and α-glucosidase activity), regulations of gut microbiota, inflammatory cytokines, and gene and protein expressions in insulin signaling pathway, as well as amelioration of DM complications. This comprehensive review provides an up-to-date overview of the hypoglycemic properties associated with tea and its bioactive components. It also delves into their potential mechanisms, offering a theoretical foundation for further research into tea's antidiabetic properties and for the development of innovative antidiabetic functional products.
{"title":"Research progress on the antidiabetic activities of tea and its bioactive components","authors":"Jianjian Gao, Dan Chen, Zhiyuan Lin, Jiakun Peng, Shuai Yu, Chuang Zhou, Huimin Jiang, Ruofan Sun, Zhi Lin, Weidong Dai","doi":"10.48130/bpr-2023-0032","DOIUrl":"https://doi.org/10.48130/bpr-2023-0032","url":null,"abstract":"Diabetes mellitus (DM) is a pressing global public health issue with a high incidence of morbidity and mortality due to its complications. Although there are many medicines available for the treatment of DM, long-term use causes various adverse effects, such as diarrhea, vomiting, and nausea. Tea, owing to its richness of diverse bioactive components including tea polyphenols, tea polysaccharides, and alkaloids, has displayed promising antidiabetic properties. Screening antidiabetic bioactive compounds derived from teas is receiving increasing attention. Epidemiological and clinical investigations have demonstrated an inverse relationship between tea consumption and the incidence of DM. Both <italic>in vitro</italic> and <italic>in vivo</italic> experiments have substantiated the hypoglycemic effects of tea and its bioactive components through several possible mechanisms, including improvement of insulin resistance, inhibition of carbohydrates digestion and absorption (inhibit <italic>α</italic>-amylase and <italic>α</italic>-glucosidase activity), regulations of gut microbiota, inflammatory cytokines, and gene and protein expressions in insulin signaling pathway, as well as amelioration of DM complications. This comprehensive review provides an up-to-date overview of the hypoglycemic properties associated with tea and its bioactive components. It also delves into their potential mechanisms, offering a theoretical foundation for further research into tea's antidiabetic properties and for the development of innovative antidiabetic functional products.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136373066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jianlong Li, Guotai Jian, Jiajia Qian, Jinghua Xue, Chengshun Liu, Yongxia Jia, Bo Zhou, Jinchi Tang, Jie Yang, Lanting Zeng
Tea plants (Camellia sinensis) are used to produce beverages that are consumed worldwide. Similar to many other plants, tea plants attacked by herbivores emit herbivore-induced plant volatiles (HIPVs) that enhance defense responses, regulate insect behavior, and send 'warning signals' to neighboring plants. However, the related mechanisms remain relatively unclear. In this study, the anti-insect effects and emission patterns of indole, which is a common HIPV synthesized in tea plants, were investigated. The anti-insect effects of indole may involve the induction of signal transduction pathways and the attraction of natural enemies. An attack by tea geometrids, which are major tea plant pests, resulted in the regular emission of indole. The analysis of plants with simulated insect feeding-induced damages showed that indole emission was higher at noon than at midnight. Additionally, significantly more indole was released by plants maintained under continuous light than by plants kept in darkness. The regular emission of indole may be related to the regular expression of the structural gene CsTSB2. The interaction between CsTSA and CsTSB2 was confirmed, further indicating CsTSB2 likely regulates the regular biosynthesis and emission of indole. The study findings may be relevant to improving environmentally friendly pest control measures in tea plantations.
{"title":"Emission pattern and anti-insect function of indole from tea plants (<i>Camellia sinensis</i>) attacked by tea geometrids","authors":"Jianlong Li, Guotai Jian, Jiajia Qian, Jinghua Xue, Chengshun Liu, Yongxia Jia, Bo Zhou, Jinchi Tang, Jie Yang, Lanting Zeng","doi":"10.48130/bpr-0023-0036","DOIUrl":"https://doi.org/10.48130/bpr-0023-0036","url":null,"abstract":"Tea plants (<italic>Camellia sinensis</italic>) are used to produce beverages that are consumed worldwide. Similar to many other plants, tea plants attacked by herbivores emit herbivore-induced plant volatiles (HIPVs) that enhance defense responses, regulate insect behavior, and send 'warning signals' to neighboring plants. However, the related mechanisms remain relatively unclear. In this study, the anti-insect effects and emission patterns of indole, which is a common HIPV synthesized in tea plants, were investigated. The anti-insect effects of indole may involve the induction of signal transduction pathways and the attraction of natural enemies. An attack by tea geometrids, which are major tea plant pests, resulted in the regular emission of indole. The analysis of plants with simulated insect feeding-induced damages showed that indole emission was higher at noon than at midnight. Additionally, significantly more indole was released by plants maintained under continuous light than by plants kept in darkness. The regular emission of indole may be related to the regular expression of the structural gene <italic>CsTSB2</italic>. The interaction between CsTSA and CsTSB2 was confirmed, further indicating CsTSB2 likely regulates the regular biosynthesis and emission of indole. The study findings may be relevant to improving environmentally friendly pest control measures in tea plantations.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135559733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
TCP transcription factors are essential for plant growth and environmental response, but little is known about their specific roles in growth, hormone response, and stress response in the tea plants. Here, CsTCP5 and CsTCP18 were cloned from the tea variety 'Longjing 43', and subcellular localization revealed that both CsTCP5 and CsTCP18 proteins were present in the nucleus. Exogenous abscisic acid (ABA) and methyl jasmonate (MeJA) had different effects on CsTCP5 and CsTCP18 expression, but both genes were repressed by drought and low-temperature treatment. Yeast-two hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays demonstrated that neither transcription factor was transcriptionally active but that each interacted with members of the CsMYB and CsJAZ families. Transgenic Arabidopsis plants overexpressing CsTCP5 had smaller cotyledons and fewer lateral roots compared with wild-type (WT) and empty vector (EV) Arabidopsis, and their root growth and germination rate were inhibited by ABA and MeJA treatment. Lateral root numbers also decreased significantly in CsTCP18-OE Arabidopsis after MeJA treatment. These results demonstrate that CsTCP5 and CsTCP18 have regulatory effects in cotyledon development, lateral root growth, and germination rate, and these effects can be modulated by ABA and MeJA. Under drought stress, the CsTCP5-OE and CsTCP18-OE lines exhibited greater survival, lower plant height, smaller rosette leaves, delayed flowering, increased activities of antioxidant enzymes, decreased MDA content, and increased proline content compared with WT and EV Arabidopsis. These findings suggest that CsTCP5 and CsTCP18 are important for tea plant growth, interact with ABA and MeJA pathways, and play roles in stress response.
{"title":"The effective role of CsTCP5 and CsTCP18 transcription factors from <i>Camellia sinensis</i> (L.) O. Kuntze under drought and low-temperature.","authors":"Huan Zhang, Xiaowen Shang, Ning Zhou, Zhaolan Han, Chen Zhang, Yuanchun Ma, Wanping Fang","doi":"10.48130/bpr-2023-0029","DOIUrl":"https://doi.org/10.48130/bpr-2023-0029","url":null,"abstract":"TCP transcription factors are essential for plant growth and environmental response, but little is known about their specific roles in growth, hormone response, and stress response in the tea plants. Here, CsTCP5 and CsTCP18 were cloned from the tea variety 'Longjing 43', and subcellular localization revealed that both CsTCP5 and CsTCP18 proteins were present in the nucleus. Exogenous abscisic acid (ABA) and methyl jasmonate (MeJA) had different effects on CsTCP5 and CsTCP18 expression, but both genes were repressed by drought and low-temperature treatment. Yeast-two hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays demonstrated that neither transcription factor was transcriptionally active but that each interacted with members of the CsMYB and CsJAZ families. Transgenic <italic>Arabidopsis</italic> plants overexpressing CsTCP5 had smaller cotyledons and fewer lateral roots compared with wild-type (WT) and empty vector (EV) <italic>Arabidopsis,</italic> and their root growth and germination rate were inhibited by ABA and MeJA treatment. Lateral root numbers also decreased significantly in CsTCP18-OE <italic>Arabidopsis</italic> after MeJA treatment. These results demonstrate that CsTCP5 and CsTCP18 have regulatory effects in cotyledon development, lateral root growth, and germination rate, and these effects can be modulated by ABA and MeJA. Under drought stress, the CsTCP5-OE and CsTCP18-OE lines exhibited greater survival, lower plant height, smaller rosette leaves, delayed flowering, increased activities of antioxidant enzymes, decreased MDA content, and increased proline content compared with WT and EV <italic>Arabidopsis</italic>. These findings suggest that CsTCP5 and CsTCP18 are important for tea plant growth, interact with ABA and MeJA pathways, and play roles in stress response.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136210005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huangqin tea (HQT), derived from the aerial parts of various Scutellaria species, in particular S. baicalensis Georgi, has a long history of traditional use in China. Its significance has grown in recent years due to its potential anti-aging, colon cancer chemopreventive, and cardiovascular protective properties. Huangqin tea source plants have identified over 295 chemical constituents, including flavonoids, essential oils, phenolic acids, sterols, diterpenes, polysaccharides, and amino acids. Pharmacological research has underscored the diverse beneficial effects of Huangqin tea and flavonoid extracts. These effects encompass anti-inflammatory, antiviral, anti-bacterial, antipyretic, and analgesic properties, along with neuroprotective effects and protection against cardiovascular and cerebrovascular diseases. Safety studies indicate that HQT is generally safe within recommended dosages and historical use. HQT presents multifaceted potential health benefits, though comprehensive research is necessary to ensure its effectiveness and safety in human applications.
{"title":"A comprehensive review of Huangqin (<i>Scutellaria baicalensis</i> Georgi) tea: Chemical composition, functional properties and safety aspects","authors":"Yining Quan, Zhenpeng Li, Xiangying Meng, Panpan Li, Yanying Wang, Chunnian He, Jie Shen","doi":"10.48130/bpr-2023-0031","DOIUrl":"https://doi.org/10.48130/bpr-2023-0031","url":null,"abstract":"Huangqin tea (HQT), derived from the aerial parts of various <italic>Scutellaria</italic> species, in particular <italic>S. baicalensis</italic> Georgi, has a long history of traditional use in China. Its significance has grown in recent years due to its potential anti-aging, colon cancer chemopreventive, and cardiovascular protective properties. Huangqin tea source plants have identified over 295 chemical constituents, including flavonoids, essential oils, phenolic acids, sterols, diterpenes, polysaccharides, and amino acids. Pharmacological research has underscored the diverse beneficial effects of Huangqin tea and flavonoid extracts. These effects encompass anti-inflammatory, antiviral, anti-bacterial, antipyretic, and analgesic properties, along with neuroprotective effects and protection against cardiovascular and cerebrovascular diseases. Safety studies indicate that HQT is generally safe within recommended dosages and historical use. HQT presents multifaceted potential health benefits, though comprehensive research is necessary to ensure its effectiveness and safety in human applications.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136367252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
URIDINE DIPHOSPHATE (UDP)-GLYCOSYLTRANSFERASES (UGT) play essential roles in modifying secondary metabolites during the plant life cycle and are also involved in the response to abiotic stresses. However, the plant UGT family is vast and the available data describing their role in abiotic stress responses is varied and intricate, so that their potential roles are often obscured. To address this, a meta-analysis was conducted to assess the effects of overexpression of UGT on various plant physiological indicators under abiotic stress. Out of the 15 plant characteristics examined in UGT overexpressing plants, 10 showed an increase of over 30% , while 2 plant characteristics decreased by more than 30%, while only 3 indices were significantly affected under non-stressed conditions. Notably, UGT had a significant and positive effect in salt-stressed plants. This study sheds light on the complex role of UGT in abiotic stress and can provide valuable guidance for future research on UGT functions and their genetic manipulation in crop breeding programs for improved abiotic stress tolerances.
{"title":"The effects of overexpressing <i>UDP-Glycosyltransferases</i> genes on the plant response to abiotic stress: A Meta-analysis","authors":"Yiwen Chen, Yu Cao, Yu Duan, Deng Deng, Qinqin Gao, Qiang Shen, Wanping Fang, Xujun Zhu","doi":"10.48130/bpr-2023-0028","DOIUrl":"https://doi.org/10.48130/bpr-2023-0028","url":null,"abstract":"URIDINE DIPHOSPHATE (UDP)-GLYCOSYLTRANSFERASES (UGT) play essential roles in modifying secondary metabolites during the plant life cycle and are also involved in the response to abiotic stresses. However, the plant <italic>UGT</italic> family is vast and the available data describing their role in abiotic stress responses is varied and intricate, so that their potential roles are often obscured. To address this, a meta-analysis was conducted to assess the effects of overexpression of <italic>UGT</italic> on various plant physiological indicators under abiotic stress. Out of the 15 plant characteristics examined in <italic>UGT</italic> overexpressing plants, 10 showed an increase of over 30% , while 2 plant characteristics decreased by more than 30%, while only 3 indices were significantly affected under non-stressed conditions. Notably, UGT had a significant and positive effect in salt-stressed plants. This study sheds light on the complex role of UGT in abiotic stress and can provide valuable guidance for future research on UGT functions and their genetic manipulation in crop breeding programs for improved abiotic stress tolerances.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135156718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yiqing Zhao, Wenjing Wang, Xihua Zhan, Mengyuan Zhang, Yao Xiao, Xinru Hou, Min Gao, Bin Xiao, Yuefang Gao
Chlorophyll biosynthesis is a crucial biological process in plants, and chlorophyll content is one of the most important traits in the yield and quality of tea. Magnesium chelatase is a conserved enzyme complex responsible for the chlorophyll biosynthesis, which composed of the subnuit of CHLI, CHLD and CHLH. In this study, there were positive correlation between the expression of CsCHLI, chlorophyll content and chloroplast structure. The CsCHLI gene structure and functional domain indicated that, its cDNA length was 1275 bp, encodes 424 amino acids, consisted of cTP, AAA+ and AAA lid domain. Meanwhile, the subcellular localization demonstrated that CsCHLI localized in chloroplasts. In addition, protein-protein interaction analysis by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that CsCHLI could interact with CsCHLI to form homodimer, or perhaps interact with CsCHLD and CsCHLH to form heterodimer. Moreover, Arabidopsis transformation displayed that overexpression of CsCHLI could restore the etiolation phenotype of the atchli1 mutant. These findings provide the mechanistic function of CsCHLI and its implications in chlorophyll biosynthesis in tea plant.
{"title":"CsCHLI plays an important role in chlorophyll biosynthesis of tea plant (<i>Camellia sinensis</i>)","authors":"Yiqing Zhao, Wenjing Wang, Xihua Zhan, Mengyuan Zhang, Yao Xiao, Xinru Hou, Min Gao, Bin Xiao, Yuefang Gao","doi":"10.48130/bpr-0023-0037","DOIUrl":"https://doi.org/10.48130/bpr-0023-0037","url":null,"abstract":"Chlorophyll biosynthesis is a crucial biological process in plants, and chlorophyll content is one of the most important traits in the yield and quality of tea. Magnesium chelatase is a conserved enzyme complex responsible for the chlorophyll biosynthesis, which composed of the subnuit of CHLI, CHLD and CHLH. In this study, there were positive correlation between the expression of <italic>CsCHLI</italic>, chlorophyll content and chloroplast structure. The <italic>CsCHLI</italic> gene structure and functional domain indicated that, its cDNA length was 1275 bp, encodes 424 amino acids, consisted of cTP, AAA+ and AAA lid domain. Meanwhile, the subcellular localization demonstrated that CsCHLI localized in chloroplasts. In addition, protein-protein interaction analysis by yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays indicated that CsCHLI could interact with CsCHLI to form homodimer, or perhaps interact with CsCHLD and CsCHLH to form heterodimer. Moreover, <italic>Arabidopsis</italic> transformation displayed that overexpression of CsCHLI could restore the etiolation phenotype of the <italic>atchli1</italic> mutant. These findings provide the mechanistic function of CsCHLI and its implications in chlorophyll biosynthesis in tea plant.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135613715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leaf photosynthesis is largely determined by anatomical features. This study aimed to reveal the quantitative effects of the anatomical structure of Coffea arabica leaves on photosynthesis. Pearson’s correlation and path analysis were used to explore the correlation between leaf structure and photosynthesis. To calculate the comprehensive evaluation value of the correlation between leaf anatomical traits and photosynthetic parameters, the Criteria Importance Through Intercriteria Correlation (CRITIC) method was used to obtain the objective weight of each photosynthetic parameter. The study revealed that leaf anatomical traits were highly significant (p <0.01) and correlated with photosynthetic parameters, suggesting that anatomical traits greatly influenced photosynthesis in C. arabica leaf. Similarly, path coefficient analysis strongly showed direct and indirect correlation of photosynthetic capacity of stomatal conductance of the leaves of C. arabica. The results of the comprehensive evaluation also indicated that leaf thickness (LT) and stomatal density (d) were the anatomical characteristics most closely related to photosynthesis. In these results, understanding the effects of the anatomical structure of coffee leaves on photosynthesis, may provide useful information for coffee breeding programs and the management of coffee plantations to optimize Photosynthetic capacity.
叶片光合作用在很大程度上是由解剖特征决定的。本研究旨在揭示阿拉比卡咖啡叶片解剖结构对光合作用的定量影响。利用Pearson相关分析和通径分析探讨了叶片结构与光合作用的相关性。为了计算叶片解剖性状与光合参数相关性的综合评价值，采用critical (Criteria Importance Through Intercriteria correlation)方法获得各光合参数的客观权值。结果表明，叶片解剖性状与光合参数呈极显著相关(p <0.01)，说明解剖性状对阿拉比卡咖啡叶片光合作用影响较大。同样，通径分析也显示了阿拉比卡叶气孔导度的光合能力与叶片的直接或间接相关。综合评价结果还表明，叶片厚度(LT)和气孔密度(d)是与光合作用关系最密切的解剖特征。因此，了解咖啡叶片解剖结构对光合作用的影响，可以为咖啡育种计划和咖啡种植园管理提供有用的信息，以优化咖啡种植园的光合能力。
{"title":"The Influence of Leaf Anatomical Traits on Photosynthesis in Catimor type Arabica coffee","authors":"Ziwei Xiao, Guanrun Ma, Xuehui Bai, Jinhong Li, Mingzhu Zhao, Linlin Su, Hua Zhou","doi":"10.48130/bpr-0023-0035","DOIUrl":"https://doi.org/10.48130/bpr-0023-0035","url":null,"abstract":"Leaf photosynthesis is largely determined by anatomical features. This study aimed to reveal the quantitative effects of the anatomical structure of <italic>Coffea arabica</italic> leaves on photosynthesis. Pearson’s correlation and path analysis were used to explore the correlation between leaf structure and photosynthesis. To calculate the comprehensive evaluation value of the correlation between leaf anatomical traits and photosynthetic parameters, the Criteria Importance Through Intercriteria Correlation (CRITIC) method was used to obtain the objective weight of each photosynthetic parameter. The study revealed that leaf anatomical traits were highly significant (<italic>p</italic> <0.01) and correlated with photosynthetic parameters, suggesting that anatomical traits greatly influenced photosynthesis in <italic>C. arabica</italic> leaf. Similarly, path coefficient analysis strongly showed direct and indirect correlation of photosynthetic capacity of stomatal conductance of the leaves of <italic>C. arabica</italic>. The results of the comprehensive evaluation also indicated that leaf thickness (LT) and stomatal density (<italic>d</italic>) were the anatomical characteristics most closely related to photosynthesis. In these results, understanding the effects of the anatomical structure of coffee leaves on photosynthesis, may provide useful information for coffee breeding programs and the management of coffee plantations to optimize Photosynthetic capacity.","PeriodicalId":223765,"journal":{"name":"Beverage Plant Research","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134889451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：