Pub Date : 2015-04-22DOI: 10.11669/CPJ.2015.08.005
Qiao-Qiao Chu, Tao Liu, S. Jia, B. Huang, Hong-Bing Huang
{"title":"Stable interference of serglycin enhances sensitivity to cisplatin in nasopharyngeal carcinoma highly metastatic cells","authors":"Qiao-Qiao Chu, Tao Liu, S. Jia, B. Huang, Hong-Bing Huang","doi":"10.11669/CPJ.2015.08.005","DOIUrl":"https://doi.org/10.11669/CPJ.2015.08.005","url":null,"abstract":"","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"47 1","pages":"676-680"},"PeriodicalIF":0.0,"publicationDate":"2015-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81110382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-06-08DOI: 10.11669/CPJ.2013.11.002
Chenguang Wang, L. Deng, C. Shi
{"title":"Pharmaceutical powders tabletability: influencing factors and improvement strategies","authors":"Chenguang Wang, L. Deng, C. Shi","doi":"10.11669/CPJ.2013.11.002","DOIUrl":"https://doi.org/10.11669/CPJ.2013.11.002","url":null,"abstract":"","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"54 1","pages":"845-849"},"PeriodicalIF":0.0,"publicationDate":"2013-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74111281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-05-22DOI: 10.11669/CPJ.2013.10.003
Song Gao, Zhengkun Chen
{"title":"The progression and prospects in research of molecular targeted therapy for pancreatic cancer","authors":"Song Gao, Zhengkun Chen","doi":"10.11669/CPJ.2013.10.003","DOIUrl":"https://doi.org/10.11669/CPJ.2013.10.003","url":null,"abstract":"","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"71 1","pages":"763-767"},"PeriodicalIF":0.0,"publicationDate":"2013-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80313368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-04-22DOI: 10.11669/CPJ.2013.08.020
Juan Zhang, Qing Zhai
{"title":"Compatibility study of insulin with polyene phosphatidylcholine in glucose injection","authors":"Juan Zhang, Qing Zhai","doi":"10.11669/CPJ.2013.08.020","DOIUrl":"https://doi.org/10.11669/CPJ.2013.08.020","url":null,"abstract":"","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"40 1","pages":"652-654"},"PeriodicalIF":0.0,"publicationDate":"2013-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83583132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tsao-Jun Su, Hsun-Shuo Chang, C. Peng, Shiow-Ju Lee, I. Chen
{"title":"Antitubercular Resorcinols and Cytotoxic Alkyl Benzoquinones from Ardisia kusukuensis","authors":"Tsao-Jun Su, Hsun-Shuo Chang, C. Peng, Shiow-Ju Lee, I. Chen","doi":"10.7019/TPJ.200912.0089","DOIUrl":"https://doi.org/10.7019/TPJ.200912.0089","url":null,"abstract":"","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"44 1","pages":"89-105"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75921844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chi-Yu Lu, H. Kou, Chia-Hsien Feng, Hsiang-Ming Chen, Hsin‐Lung Wu
The fat content in food is concerned by genera l public, because over uptake of some saturated fatty acids could lead to heart related disease. Therefore, practical method for the analysis of fatty acids in food is essential. In this article, a highly sensitive HPLC method is described for the quantitative analysis of long-chain free fatty acids in yogurt, including lauric, myristic, palmitic, stearic, palmitoleic, oleic and linoleic acids. The fatty acids were labeled with a fluorophore by reacting with 2-(2-naphthoxy) ethyl 2-(piperidino) ethanesulfonate (NOEPES) at 95℃ for 0.5 h in the presence of 18-crown-6 and solid potassium carbonate. The resulting naphthoxyethyl derivatives were separated on a C8 column with a mobile phase of methanol-water (92:8, v/v) and detected fluorimetrically (excitation at 235 nm and detection at 350 nm). Before labeling, the fatty acids in yogurt were extracted with toluene and the resulting toluene extract was directly subject to the labeling without time-consuming steps of solvent evaporation and solvent replacement. The fluorimetric HPLC analysis of fatty acids could be measured at few μ M levels in yogurt. The results indicate that the content of free fatty acids in low-fat yogurt is lower than that in plain yogurt from the same food producer.
{"title":"A Highly Sensitive Method for the Analysis of Long-Chain Free Fatty Acids in Yogurt by High Performance Liquid Chromatography with Fluorimetric Detection","authors":"Chi-Yu Lu, H. Kou, Chia-Hsien Feng, Hsiang-Ming Chen, Hsin‐Lung Wu","doi":"10.7019/TPJ.200912.0065","DOIUrl":"https://doi.org/10.7019/TPJ.200912.0065","url":null,"abstract":"The fat content in food is concerned by genera l public, because over uptake of some saturated fatty acids could lead to heart related disease. Therefore, practical method for the analysis of fatty acids in food is essential. In this article, a highly sensitive HPLC method is described for the quantitative analysis of long-chain free fatty acids in yogurt, including lauric, myristic, palmitic, stearic, palmitoleic, oleic and linoleic acids. The fatty acids were labeled with a fluorophore by reacting with 2-(2-naphthoxy) ethyl 2-(piperidino) ethanesulfonate (NOEPES) at 95℃ for 0.5 h in the presence of 18-crown-6 and solid potassium carbonate. The resulting naphthoxyethyl derivatives were separated on a C8 column with a mobile phase of methanol-water (92:8, v/v) and detected fluorimetrically (excitation at 235 nm and detection at 350 nm). Before labeling, the fatty acids in yogurt were extracted with toluene and the resulting toluene extract was directly subject to the labeling without time-consuming steps of solvent evaporation and solvent replacement. The fluorimetric HPLC analysis of fatty acids could be measured at few μ M levels in yogurt. The results indicate that the content of free fatty acids in low-fat yogurt is lower than that in plain yogurt from the same food producer.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"29 1","pages":"65-72"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83096087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Hsiao, Y. Yen, Chun-Nin Lee, Weng‐Foung Huang, Hsiang-Yin Chen
Antituberculosis therapy frequently causes hepatotoxicity. The study is to evaluate the appropriateness of liver function monitoring during antituberculosis therapy. Two hundred forty five patients treated with antituberculosis agents were included. Abnormal baseline liver function (LFT) was the most significant risk factor for developing hepatotoxicity during the therapy (adjusted OR 23.48; 95% CI: 9.74-56.61). However, the baseline aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were only checked in 76.2% and 75.4% subjects in the hepatotoxic group; and even lower to 58.5% and 57.8% for the non-hepatotoxic group. Although smoking, severe drinking, age, gender and concurrent diseases were significant risk factors, the logistic regression showed that only abnormal baseline LFT (adjusted OR 2.21; 95% CI: 1.22-4.02) and age (adjusted OR 1.02; 95% CI: 1.01-1.04) were determinants of patients receiving follow-up liver function tests (LFTs). Effective strategies to improve the monitoring of liver function should be established to ensure patient safety.
{"title":"Prevention and Monitoring of Hepatotoxicity among Patients Receiving Antituberculosis Medications","authors":"F. Hsiao, Y. Yen, Chun-Nin Lee, Weng‐Foung Huang, Hsiang-Yin Chen","doi":"10.7019/TPJ.200912.0107","DOIUrl":"https://doi.org/10.7019/TPJ.200912.0107","url":null,"abstract":"Antituberculosis therapy frequently causes hepatotoxicity. The study is to evaluate the appropriateness of liver function monitoring during antituberculosis therapy. Two hundred forty five patients treated with antituberculosis agents were included. Abnormal baseline liver function (LFT) was the most significant risk factor for developing hepatotoxicity during the therapy (adjusted OR 23.48; 95% CI: 9.74-56.61). However, the baseline aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were only checked in 76.2% and 75.4% subjects in the hepatotoxic group; and even lower to 58.5% and 57.8% for the non-hepatotoxic group. Although smoking, severe drinking, age, gender and concurrent diseases were significant risk factors, the logistic regression showed that only abnormal baseline LFT (adjusted OR 2.21; 95% CI: 1.22-4.02) and age (adjusted OR 1.02; 95% CI: 1.01-1.04) were determinants of patients receiving follow-up liver function tests (LFTs). Effective strategies to improve the monitoring of liver function should be established to ensure patient safety.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"7 1","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82291854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. El-Azazy, Magda Y El-Mammli, A. Shalaby, M. Ayad
A new spectrophotometric method has been described for the assay of aciclovir, fluconazole, ramipril and secnidazole based on formation of insoluble molecular complexes with molybdophosphoric acid (MPA) under acidic conditions. As a second step, a colour reaction has been combined to determine MI’A, released from the complex (and the studied drugs in turn), using a chromogenic reductant; cobalt (Ⅱ)-EDTA complex to produce molybdenum blue Mo(subscript 5+). The colour of the produced Mo(subscript 5+) is measured at 700-710 nm. Appropriate conditions were established for the precipitation and the colour reactions to obtain maximum sensitivity. Under the proposed conditions, this method is applicable over concentration range of 40-580μg ML^(-1). The results demonstrated that the proposed method is equally accurate, precise and reproducible as the official or reported methods thus it is recommended for quality control and routine analysis where time, cost effectiveness and high specificity of analytical techniques are of great importance.
{"title":"Application of Cobalt (II)-EDTA Complex as a Reductant for Molybdophosphoric Acid Used for the Spectrophotometric Assay of Aciclovir, Fluconazole, Ramipril and Secnidazole","authors":"M. El-Azazy, Magda Y El-Mammli, A. Shalaby, M. Ayad","doi":"10.7019/TPJ.200909.0043","DOIUrl":"https://doi.org/10.7019/TPJ.200909.0043","url":null,"abstract":"A new spectrophotometric method has been described for the assay of aciclovir, fluconazole, ramipril and secnidazole based on formation of insoluble molecular complexes with molybdophosphoric acid (MPA) under acidic conditions. As a second step, a colour reaction has been combined to determine MI’A, released from the complex (and the studied drugs in turn), using a chromogenic reductant; cobalt (Ⅱ)-EDTA complex to produce molybdenum blue Mo(subscript 5+). The colour of the produced Mo(subscript 5+) is measured at 700-710 nm. Appropriate conditions were established for the precipitation and the colour reactions to obtain maximum sensitivity. Under the proposed conditions, this method is applicable over concentration range of 40-580μg ML^(-1). The results demonstrated that the proposed method is equally accurate, precise and reproducible as the official or reported methods thus it is recommended for quality control and routine analysis where time, cost effectiveness and high specificity of analytical techniques are of great importance.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"272 1","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77098601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The In vitro metabolism of an arylalkylamine analogue (McN-5691), a calcium entry blocker, was conducted after 0 min and 60 min incubations with the rat hepatic S9 fraction in the presence of an NADPH-generating system. Unchanged McN-5691 (31% of the sample) plus 15 metabolites from the 60min incubation were profiled, quantified, and tentatively identified on the basis of API-MS and MS/MS data. The formation McN-5691 metabolites are via the following four metabolic pathways: A. N-demethylation, B. O-demethylation, C. phenylhydroxylation, and D. oxidative N-dealkylation. Pathways A to C formed 9 major/moderate/minor metabolites, N-desmethyl (MI, 42% of the sample), 4-O-desmethyl (M2, 6%), 4'-O-desmethyl (M3, 6%), OH-phenyl (M4, 1%), O,O-didesmethyl (M5, 1%), and OH-phenyl-M1 (M6, 1%) McN-5691s, and 3 other minor phenylhydroxyl/N-desmethyl/O-desmethyl-combined McN-5691 metabolites (M7-M9, ≤ 1%). Pathway C produced a N-dealkylated metabolite, M10 (2%); in conjunction with pathways A to C yielded 5 minor oxidized N-dealkylated metabolites M11-M15 (each, ≤ 1-2%). Rat appeared to metabolize McN-5691 extensively in this hepatic system.
在nadph生成系统存在的情况下，将一种芳基烷基胺类似物(McN-5691)(钙进入阻断剂)与大鼠肝脏S9部分孵育0分钟和60分钟后进行体外代谢。在API-MS和MS/MS数据的基础上，对未改变的McN-5691(31%的样本)和60分钟孵育后的15种代谢物进行了分析、定量和初步鉴定。McN-5691代谢产物的形成主要通过四种代谢途径:A. n -去甲基化，B. o -去甲基化，C.苯基羟基化和D.氧化n -脱烷基。途径A到C形成了9个主要/中等/次要代谢物，n -去甲基(MI，占样本的42%)、4-O-去甲基(M2, 6%)、4'-O-去甲基(M3, 6%)、oh -苯基(M4, 1%)、O,O-二甲基(M5, 1%)和oh -苯基- m1 (M6, 1%) McN-5691，以及3个其他次要苯基羟基/ n -去甲基/O-去甲基组合McN-5691代谢物(M7-M9，≤1%)。途径C产生n -脱烷基代谢物M10 (2%);与途径A到C结合产生5种次要氧化n -脱烷基代谢物M11-M15(每种≤1-2%)。大鼠似乎在这个肝脏系统中广泛代谢McN-5691。
{"title":"The Metabolism of a Calcium Entry Blocker, Arylalkylamine Analogue, in the In-Vitro Rat Hepatic System","authors":"W. Wu, L. A. Mckown","doi":"10.7019/TPJ.200909.0053","DOIUrl":"https://doi.org/10.7019/TPJ.200909.0053","url":null,"abstract":"The In vitro metabolism of an arylalkylamine analogue (McN-5691), a calcium entry blocker, was conducted after 0 min and 60 min incubations with the rat hepatic S9 fraction in the presence of an NADPH-generating system. Unchanged McN-5691 (31% of the sample) plus 15 metabolites from the 60min incubation were profiled, quantified, and tentatively identified on the basis of API-MS and MS/MS data. The formation McN-5691 metabolites are via the following four metabolic pathways: A. N-demethylation, B. O-demethylation, C. phenylhydroxylation, and D. oxidative N-dealkylation. Pathways A to C formed 9 major/moderate/minor metabolites, N-desmethyl (MI, 42% of the sample), 4-O-desmethyl (M2, 6%), 4'-O-desmethyl (M3, 6%), OH-phenyl (M4, 1%), O,O-didesmethyl (M5, 1%), and OH-phenyl-M1 (M6, 1%) McN-5691s, and 3 other minor phenylhydroxyl/N-desmethyl/O-desmethyl-combined McN-5691 metabolites (M7-M9, ≤ 1%). Pathway C produced a N-dealkylated metabolite, M10 (2%); in conjunction with pathways A to C yielded 5 minor oxidized N-dealkylated metabolites M11-M15 (each, ≤ 1-2%). Rat appeared to metabolize McN-5691 extensively in this hepatic system.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"67 1","pages":"53-64"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88410601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shu-fen Lee, Yiji Chen, Choung-Hui Lee, Chiareiy Liu
The purpose of this study was to investigate the discrepancy of gene expression of cells treated with heroin or morphine and codeine. Firstly, microarray was used to screen for the difference of genes, which are different in expression between heroin and non-drug users. Compared to non- drug user, 80 genes were distinctly induced in drug users. Only one gene, APOBEC3A gene was up-regulation, and the others were down-regulation. The expressions of seven out of the eighty affected genes, including CAMP, MPP1, OGFR, PARP10 RGS12, HES7, and, APOBEC3A were then validated by real-time RT-PCR amplification by using a cultured cell model. Ramos cells treated with morphine and codeine indicated that both drugs could down regulate CAMP gene expression. Ramos cells treated with morphine showed that the expression of MPP1, RGSI2, OGFR, P4RP10, and HES7 resulted in down-regulation, but cells treated with codeine revealed no induction. The APOBEC3A mRNA was not influenced in Ramos cells treated with morphine, but up-regulated in codeine-treated cells. According to these results, we hypothesize that there arc different gene expressions between cells exposed to morphine and codeine.
{"title":"The Difference of Gene Expression between Morphine and Codeine on Ramos Cells","authors":"Shu-fen Lee, Yiji Chen, Choung-Hui Lee, Chiareiy Liu","doi":"10.7019/TPJ.200909.0001","DOIUrl":"https://doi.org/10.7019/TPJ.200909.0001","url":null,"abstract":"The purpose of this study was to investigate the discrepancy of gene expression of cells treated with heroin or morphine and codeine. Firstly, microarray was used to screen for the difference of genes, which are different in expression between heroin and non-drug users. Compared to non- drug user, 80 genes were distinctly induced in drug users. Only one gene, APOBEC3A gene was up-regulation, and the others were down-regulation. The expressions of seven out of the eighty affected genes, including CAMP, MPP1, OGFR, PARP10 RGS12, HES7, and, APOBEC3A were then validated by real-time RT-PCR amplification by using a cultured cell model. Ramos cells treated with morphine and codeine indicated that both drugs could down regulate CAMP gene expression. Ramos cells treated with morphine showed that the expression of MPP1, RGSI2, OGFR, P4RP10, and HES7 resulted in down-regulation, but cells treated with codeine revealed no induction. The APOBEC3A mRNA was not influenced in Ramos cells treated with morphine, but up-regulated in codeine-treated cells. According to these results, we hypothesize that there arc different gene expressions between cells exposed to morphine and codeine.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"13 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83799515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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