Yuh-Chiang Shen, Chieh‐fu Chen, Yea-Hwey Wang, T. Chang, C. Chou
We have previously isolated some lanostane- and ergostane-type compounds from the fruiting bodies of Antrodia camphorata and Antrodia sp. in our laboratory. In this study, an ergostane-type chemical, named antcin C (Ant C), and three lanostane-type chemicals including sulphurenic acid (SA), eburicoic acid (EA), and dehydroeburicoic acid (DeEA), were examined for their immunomodulating effect against reactive oxygen species (ROS) production induced by N-formyl-meth-ionyl-leucyl-phenylalanine (fMLP) or phorbol-1 2-myristate-1 3-acetate (PMA) in peripheral human neutrophils (PMN) or mononuclear cells (MNC). ROS production by activated-leukocytes is an important inflammatory mediator and also signals immuno-responses. Pretreatment with these four compounds concentration-dependently diminished fMLP- and PMA-induced ROS production in PMN, as measured by lucigenin-amplified chemiluminescence, with IC50 values (jiM) of 16.9±4.2, 43.9±14 and 144.8±13 for Ant C, EA and DeEA, respectively. These compounds were relatively less effective in the inhibition of ROS production by MNC with IC50 values around 200 jiM. The anti-oxidative actions of these drugs were not due to cytotoxic effects because no significant difference in cell viability was observed as compared to the vehicle control group. In summary, our data suggests that inhibition of ROS production in leukocytes by these active principles could be responsible for the immuno-modulating effects of A. camphorata.
{"title":"Evaluation of the Immuno-modulating Activity of Some Active Principles Isolated from the Fruiting Bodies of Antrodia camphorata","authors":"Yuh-Chiang Shen, Chieh‐fu Chen, Yea-Hwey Wang, T. Chang, C. Chou","doi":"10.7019/CPJ.200310.0313","DOIUrl":"https://doi.org/10.7019/CPJ.200310.0313","url":null,"abstract":"We have previously isolated some lanostane- and ergostane-type compounds from the fruiting bodies of Antrodia camphorata and Antrodia sp. in our laboratory. In this study, an ergostane-type chemical, named antcin C (Ant C), and three lanostane-type chemicals including sulphurenic acid (SA), eburicoic acid (EA), and dehydroeburicoic acid (DeEA), were examined for their immunomodulating effect against reactive oxygen species (ROS) production induced by N-formyl-meth-ionyl-leucyl-phenylalanine (fMLP) or phorbol-1 2-myristate-1 3-acetate (PMA) in peripheral human neutrophils (PMN) or mononuclear cells (MNC). ROS production by activated-leukocytes is an important inflammatory mediator and also signals immuno-responses. Pretreatment with these four compounds concentration-dependently diminished fMLP- and PMA-induced ROS production in PMN, as measured by lucigenin-amplified chemiluminescence, with IC50 values (jiM) of 16.9±4.2, 43.9±14 and 144.8±13 for Ant C, EA and DeEA, respectively. These compounds were relatively less effective in the inhibition of ROS production by MNC with IC50 values around 200 jiM. The anti-oxidative actions of these drugs were not due to cytotoxic effects because no significant difference in cell viability was observed as compared to the vehicle control group. In summary, our data suggests that inhibition of ROS production in leukocytes by these active principles could be responsible for the immuno-modulating effects of A. camphorata.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"1 1","pages":"313-318"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84734980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Moscatilin and a series of bib enzyl derivatives were synthesized from appropriate aromatic a1dehydes and phosphonium salts through the Wittig reaction. The antioxidative and cytotoxic activities of these bibenzyl derivatives were evaluated.
{"title":"Synthesis of antioxidative moscatilin and its bibenzyl derivatives","authors":"Chien‐Chih Chen, Yu-Ling Huang, Y. Shiao, Ray-Ling Huang, Tai-Ti Fu, Chien-Chang Shen","doi":"10.7019/CPJ.200310.0381","DOIUrl":"https://doi.org/10.7019/CPJ.200310.0381","url":null,"abstract":"Moscatilin and a series of bib enzyl derivatives were synthesized from appropriate aromatic a1dehydes and phosphonium salts through the Wittig reaction. The antioxidative and cytotoxic activities of these bibenzyl derivatives were evaluated.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"35 1","pages":"381-390"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85115159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ko, K. Chen, C. Chou, Yi-Ju Chang, Guei‐Jane Wang, Chieh‐fu Chen
In this study, a high performance liquid chromatographic method was developed for simultaneous determination of four bioactive components in T ruticarpum collecting from various flowering seasons. Our results demonstrate that the component synephrine (IV) was only found in flower bud and flower. During the season of flower and unripe fruit, the contents of dehydroevodiamine (I), evodiamine (II), and rutaecarpine (III) were found to increase with the development time of flower and fruit, upon ripening of the fruit, the amount of these three components were found to be decreasing with time. Therefore, the time of harvesting is an important factor to control the quality of herbal drugs.
{"title":"Quantitatively Evaluation of Bioactive Components of Evodia Rutaecarpa (Tetradium Ruticarpum) in Different Harvesting Times","authors":"H. Ko, K. Chen, C. Chou, Yi-Ju Chang, Guei‐Jane Wang, Chieh‐fu Chen","doi":"10.7019/CPJ.200310.0305","DOIUrl":"https://doi.org/10.7019/CPJ.200310.0305","url":null,"abstract":"In this study, a high performance liquid chromatographic method was developed for simultaneous determination of four bioactive components in T ruticarpum collecting from various flowering seasons. Our results demonstrate that the component synephrine (IV) was only found in flower bud and flower. During the season of flower and unripe fruit, the contents of dehydroevodiamine (I), evodiamine (II), and rutaecarpine (III) were found to increase with the development time of flower and fruit, upon ripening of the fruit, the amount of these three components were found to be decreasing with time. Therefore, the time of harvesting is an important factor to control the quality of herbal drugs.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"142 1","pages":"305-312"},"PeriodicalIF":0.0,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75514901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Investigation of the chemical composition of the water soluble fraction of the methanolic extract of the roots of Eurycoma longifolia, a medicinal plant popularly known as ”Tongkat Ali”, afforded three quassinoid diterpenoids, sugar and amino acid fractions. The sugar fraction was analyzed for its qualitative and quantitative carbohydrate con tent and glucose and fructose were identified. Quantification of these natural free sugars was achieved by HPLC using refractive index detection. The total amino acid concentration and the amino acid pattern, i.e., the relative proportion of each amino acid to the total amino acids, in the amino acid fraction were analyzed using an amino acid analyzer. Twenty five amino acids were identified. The predominant amino acids were alanine, proline, arginine, and serine summing up to 53.39% of the total. The isolated quassinoid diterpenes exhibited potent cytotoxicity and antimalarial activity.
{"title":"Characterization of the Water Soluble Fraction from the Root Extract of Eurycoma Longifolia","authors":"P. Kuo, A. G. Damu, Tian-Shung Wu","doi":"10.7019/CPJ.200308.0257","DOIUrl":"https://doi.org/10.7019/CPJ.200308.0257","url":null,"abstract":"Investigation of the chemical composition of the water soluble fraction of the methanolic extract of the roots of Eurycoma longifolia, a medicinal plant popularly known as ”Tongkat Ali”, afforded three quassinoid diterpenoids, sugar and amino acid fractions. The sugar fraction was analyzed for its qualitative and quantitative carbohydrate con tent and glucose and fructose were identified. Quantification of these natural free sugars was achieved by HPLC using refractive index detection. The total amino acid concentration and the amino acid pattern, i.e., the relative proportion of each amino acid to the total amino acids, in the amino acid fraction were analyzed using an amino acid analyzer. Twenty five amino acids were identified. The predominant amino acids were alanine, proline, arginine, and serine summing up to 53.39% of the total. The isolated quassinoid diterpenes exhibited potent cytotoxicity and antimalarial activity.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"73 1","pages":"257-265"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90378808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Dixit, Jih-Jung Chen, T. Ishikawa, I. Tsai, I. Chen
Five new alkyl phenols, virenols A~E (1~5), were isolated from the leaves of Ardisia virens. The structures of these compounds were determined by spectral analysis.
从紫荆叶中分离到5个新的烷基酚，分别为毒酚A~E(1~5)。通过光谱分析确定了这些化合物的结构。
{"title":"Alkyl Phenols from the Leaves of Formosan Ardisia Virens","authors":"A. Dixit, Jih-Jung Chen, T. Ishikawa, I. Tsai, I. Chen","doi":"10.7019/CPJ.200308.0273","DOIUrl":"https://doi.org/10.7019/CPJ.200308.0273","url":null,"abstract":"Five new alkyl phenols, virenols A~E (1~5), were isolated from the leaves of Ardisia virens. The structures of these compounds were determined by spectral analysis.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"54 1","pages":"273-278"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77173844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinese Materia Medica (CMM), in eluding herbal and traditional medicines, has been used for centuries by Asian peoples to treat disease and main tam health. CMM has also played an important role as the basis of numerous new leads for modern ”single-component” drug discovery and development. How ever, modern scientific technology should be applied not only to the discovery of single bioactive lead com pounds, but also to the development of active fractions and effective and safe herbal prescriptions as new medicines and high quality dietary supplements. This review will discuss modern research routes to wards the discovery and development of bioactive natural products and their analogs as new medicines, with emphasis on those single pure com pounds developed in the author's laboratory as anticancer and anti-AIDS clinical trials candidates. In addition, this brief re view will also discuss the quality control methods for the production of standardized new medicines, as well as the introduction of modern research technologies, such as proteomics, for the generation of new medicines from CMM in the post genomic era.
{"title":"Modern research routes to new medicines from Chinese Materia Medica","authors":"K. Lee","doi":"10.7019/CPJ.200308.0221","DOIUrl":"https://doi.org/10.7019/CPJ.200308.0221","url":null,"abstract":"Chinese Materia Medica (CMM), in eluding herbal and traditional medicines, has been used for centuries by Asian peoples to treat disease and main tam health. CMM has also played an important role as the basis of numerous new leads for modern ”single-component” drug discovery and development. How ever, modern scientific technology should be applied not only to the discovery of single bioactive lead com pounds, but also to the development of active fractions and effective and safe herbal prescriptions as new medicines and high quality dietary supplements. This review will discuss modern research routes to wards the discovery and development of bioactive natural products and their analogs as new medicines, with emphasis on those single pure com pounds developed in the author's laboratory as anticancer and anti-AIDS clinical trials candidates. In addition, this brief re view will also discuss the quality control methods for the production of standardized new medicines, as well as the introduction of modern research technologies, such as proteomics, for the generation of new medicines from CMM in the post genomic era.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"324 1","pages":"221-230"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76071743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Oyama, K. Bastow, Y. Tachibana, Y. Shirataki, S. Yamaguchi, G. Cragg, Tian-Shung Wu, K. Lee
Two novel prenylated acetophenone dimers, acrofoliones A (1) and B (2), were isolated from Acronychia trifoliolata (Rutaceae) to gether with a known cytotoxic principle, acrovestone (3). The new structures were determined from NMR and MS spectroscopic anal y sis. Com pounds 1 and 2 showed only moderate cytotoxicity against HCT-8 and KB cells with ED50 values of 4.3 and 4.8 μg/mL, respectively; how ever, both compounds demonstrated selectivity against MOLT-4 and RPMI-8225 leukemia cell lines.
{"title":"Antitumor Agents 225. Acrofoliones A and B, Two Novel Cytotoxic Acetophenone Dimers from Acronychia Trifoliolata","authors":"M. Oyama, K. Bastow, Y. Tachibana, Y. Shirataki, S. Yamaguchi, G. Cragg, Tian-Shung Wu, K. Lee","doi":"10.7019/CPJ.200308.0239","DOIUrl":"https://doi.org/10.7019/CPJ.200308.0239","url":null,"abstract":"Two novel prenylated acetophenone dimers, acrofoliones A (1) and B (2), were isolated from Acronychia trifoliolata (Rutaceae) to gether with a known cytotoxic principle, acrovestone (3). The new structures were determined from NMR and MS spectroscopic anal y sis. Com pounds 1 and 2 showed only moderate cytotoxicity against HCT-8 and KB cells with ED50 values of 4.3 and 4.8 μg/mL, respectively; how ever, both compounds demonstrated selectivity against MOLT-4 and RPMI-8225 leukemia cell lines.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"73 1","pages":"239-245"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85601080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The chemical constituents in the roots of Cassia fistula L. were investigated with column chromatography. Nine teen compounds including seven anthraquinones, chrysophanol (1), physcion (2), emodin (3), aloe-emodin (4), 11-acetylaloe-emodin (5), rhein (6), citreorosein (7); four flavan-3-ols, (-)-epiafzelechin (8), (+)-afzelechin (9), (-)-epicatechin (10), (+)-catechin (11); three sterols, a mixture of β-sitosterol (12) and stigmasterol (13), β-sitosterol-3-O-β-glucopyanoside (14); one triterpene, lupeol (15); and four gly cer ides, a mixture of glycerol-1-tetraeicosanoate (16) and glycerol-1-pentaeicosanoate (17), trimyristin (18), and glyceryl trilinolate (19) were determined by spectroscopic analysis.
{"title":"Chemical constituents from the roots of Cassia fistula L.","authors":"Ching-Kuo Lee, Y. Chung, F. Hsu, Y. Kuo","doi":"10.7019/CPJ.200308.0231","DOIUrl":"https://doi.org/10.7019/CPJ.200308.0231","url":null,"abstract":"The chemical constituents in the roots of Cassia fistula L. were investigated with column chromatography. Nine teen compounds including seven anthraquinones, chrysophanol (1), physcion (2), emodin (3), aloe-emodin (4), 11-acetylaloe-emodin (5), rhein (6), citreorosein (7); four flavan-3-ols, (-)-epiafzelechin (8), (+)-afzelechin (9), (-)-epicatechin (10), (+)-catechin (11); three sterols, a mixture of β-sitosterol (12) and stigmasterol (13), β-sitosterol-3-O-β-glucopyanoside (14); one triterpene, lupeol (15); and four gly cer ides, a mixture of glycerol-1-tetraeicosanoate (16) and glycerol-1-pentaeicosanoate (17), trimyristin (18), and glyceryl trilinolate (19) were determined by spectroscopic analysis.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"53 1","pages":"231-237"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76918658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One new triterpene, cycloart-24β-methoxy-3β-ol (1), and seven known com pounds, were isolated from the whole herb of Euphorbia makinoi. Their structures were established by spectral methods, and their cytotoxic activities were evaluated against three different cell lines. Com pound 1 showed marginal cytotoxic activity against the three human cancer cell lines while 3 showed marginal cytotoxic activity against Hep 3B and HT-29 cells in vitro.
{"title":"The Cytotoxic Constitunets of Euphorbia Makinoi","authors":"J. Weng, H. Su, M. Yen, S. Won, Chun-nan Lin","doi":"10.7019/CPJ.200308.0267","DOIUrl":"https://doi.org/10.7019/CPJ.200308.0267","url":null,"abstract":"One new triterpene, cycloart-24β-methoxy-3β-ol (1), and seven known com pounds, were isolated from the whole herb of Euphorbia makinoi. Their structures were established by spectral methods, and their cytotoxic activities were evaluated against three different cell lines. Com pound 1 showed marginal cytotoxic activity against the three human cancer cell lines while 3 showed marginal cytotoxic activity against Hep 3B and HT-29 cells in vitro.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"34 1","pages":"267-272"},"PeriodicalIF":0.0,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89921319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Abdel-salam, G. Enan, F. Serry, M. Ghonaim, Hemmat K. Abdel-Latif, C. Hollenberg
An expression plasmid was constructed and transformed to the methylotrophic yeast Hansenulapolyinorpha to produce the kappa chain peptide of IgG antibody (MAK33). Hansenula polymorpha appears to be one of the most efficient host cells for the expression of genetically engineered antibody genes. Although the rate of transformation of this yeast by polyethylene glycol was low (3-5 cells/1.0μg plasmid DNA), yet the transformants showed high mitotic stability for more than 100 generations. The expression plasmid was integrated within the yeast genome at one or more integrations site(s) with low and multicopy-number plasmids via a non-homologous integration mechanism. An N-terminal glucoamylase gene fragment was linked to the light chain (kappa) gene of the Fab derivative of the MAK33 antibody. Prepro-alpha factor of the yeast Saceharomyces cerevisiae was inserted into the plasmid between the glucoamylase and light chain genes as a secretion signal sequence for the light chain peptide. The kappa chain was produced at 50mg/L free protein in the culture medium and 500mg/L entrapped within the cells, amounting to 550mg/L and representing about 10% of the total cell protein. The prepro-a factor was shown to be incompletely processed in Hansenulapolyinorpha and the pro-segment accompanied the light chain peptide.
{"title":"Expression of the Kappa (K) Light Chain of Mouse Anticreatine Kinase-M (MAK33) Antibody in the Yeast Hansenula Polymorpha","authors":"H. Abdel-salam, G. Enan, F. Serry, M. Ghonaim, Hemmat K. Abdel-Latif, C. Hollenberg","doi":"10.7019/CPJ.200306.0197","DOIUrl":"https://doi.org/10.7019/CPJ.200306.0197","url":null,"abstract":"An expression plasmid was constructed and transformed to the methylotrophic yeast Hansenulapolyinorpha to produce the kappa chain peptide of IgG antibody (MAK33). Hansenula polymorpha appears to be one of the most efficient host cells for the expression of genetically engineered antibody genes. Although the rate of transformation of this yeast by polyethylene glycol was low (3-5 cells/1.0μg plasmid DNA), yet the transformants showed high mitotic stability for more than 100 generations. The expression plasmid was integrated within the yeast genome at one or more integrations site(s) with low and multicopy-number plasmids via a non-homologous integration mechanism. An N-terminal glucoamylase gene fragment was linked to the light chain (kappa) gene of the Fab derivative of the MAK33 antibody. Prepro-alpha factor of the yeast Saceharomyces cerevisiae was inserted into the plasmid between the glucoamylase and light chain genes as a secretion signal sequence for the light chain peptide. The kappa chain was produced at 50mg/L free protein in the culture medium and 500mg/L entrapped within the cells, amounting to 550mg/L and representing about 10% of the total cell protein. The prepro-a factor was shown to be incompletely processed in Hansenulapolyinorpha and the pro-segment accompanied the light chain peptide.","PeriodicalId":22409,"journal":{"name":"The Chinese Pharmaceutical Journal","volume":"36 1","pages":"197-206"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86983030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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