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Impact of Heat Stress on Growth Performance and some Blood and Physiological Parameters of Suckling Friesian Calves in Egypt 热应激对埃及哺乳黑黑马犊牛生长性能及部分血液和生理指标的影响
Pub Date : 2021-01-01 DOI: 10.26502/ijpaes.202121
Gaafar Hma, El-Nahrawy Mm, Mesbah Ra, Shams A Sh, Sayed Sk, A. Badr
Forty newly born male and female Friesian calves including 20 calves during the winter season and 20 calves summer season 2020, were used to study the effect of heat stress on live body weight, body weight gain, feed intake, feed and economic efficiency during suckling period. Ambient temperature, relative humidity and temperature humidity index were markedly higher during summer in compression with winter season. Digestibility coefficients of all nutrients (DM, OM, CP, CF, EE and NFE) and feeding values (TDN and DCP) reduced significantly (P<0.05) for summer ration compared to winter ration. Ruminal pH value and ammonia nitrogen concentration were higher significantly (P<0.05), however total VFA concentration was lower significantly (P<0.05) for summer than winter season. Concentrations of serum total protein, globulin and glucose were higher significantly (P<0.05), however, albumin to globulin ratio, concentrations of urea and creatinine and activity of AST and ALT activity were lower significantly (P<0.05) for winter season compared to summer season. Hemoglobin (HGB) concentration and counts of white blood cells (WBC) and red blood cells (RBC) were declined significantly (P<0.05), however, haematocrit percentage (HCT), mean cellular volume (MCV), mean cellular hemoglobin (MCH) and mean cellular hemoglobin concentration (MCHC), were raised significantly (P<0.05) for summer season than those of winter season. Intake of TDN, CP and DCP were higher significantly (P<0.05) for winter season than those of summer season. Weaning weight, total weight gain and average daily gain were higher significantly (P<0.05) for winter season in comparison with summer season. The amounts of DM, TDN, CP and DCP per kg weight gain were lower significantly (P<0.05) for summer season compared to winter season. Feed cost per kg weight gain was higher significantly (P<0.05), however, output of ADG, net revenue and economic efficiency were higher significantly (P<0.05) for winter season than those of summer season. All physiological parameters such as rectum temperature (RT), skin temperature (ST), respiration rate (RR) and pulse rate (PR) were higher significantly (P < 0.05) during the summer compared to the winter season.
本试验选用2020年冬季和夏季各20头初生雄性和雌性弗里西亚犊牛40头,研究了热应激对哺乳期活重、增重、采食量、饲料和经济效益的影响。夏季环境温度、相对湿度和温湿指数明显高于冬季。与冬季日粮相比,夏季日粮各营养物质(DM、OM、CP、CF、EE和NFE)消化系数和饲粮值(TDN和DCP)显著降低(P<0.05)。夏季瘤胃pH值和氨氮浓度显著高于冬季(P<0.05),总VFA浓度显著低于冬季(P<0.05)。血清总蛋白、球蛋白和葡萄糖浓度显著高于夏季(P<0.05),白蛋白/球蛋白比、尿素和肌酐浓度以及AST和ALT活性显著低于夏季(P<0.05)。夏季猪的血红蛋白(HGB)浓度、白细胞(WBC)和红细胞(RBC)计数显著降低(P<0.05),红细胞压积率(HCT)、平均细胞体积(MCV)、平均细胞血红蛋白(MCH)和平均细胞血红蛋白浓度(MCHC)显著高于冬季(P<0.05)。冬季TDN、CP和DCP摄取量显著高于夏季(P<0.05)。冬季断奶重、总增重和平均日增重显著高于夏季(P<0.05)。每公斤增重DM、TDN、CP和DCP值夏季显著低于冬季(P<0.05)。冬季的每公斤增重成本显著高于夏季(P<0.05),但平均日增重产量、净收入和经济效益显著高于夏季(P<0.05)。直肠温度(RT)、皮肤温度(ST)、呼吸速率(RR)、脉搏率(PR)等生理指标夏季均显著高于冬季(P < 0.05)。
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引用次数: 3
Assesment of Sequestration Potential and Biomass Accumulation of Managed Mangrove Plantations of Mida Creek, Kilifi County Kenya 肯尼亚基利菲县米达溪红树林人工林固存潜力和生物量积累评价
Pub Date : 2021-01-01 DOI: 10.26502/IJPAES.202104
Ogola Kevin Omollo, N. Dharani, B. Okeyo
Mangrove forests play a very significant role along the coastal environment throughout the tropical coast. They provide ecosystem services that are able to sustain both flora and faunal organisms found in such ecosystems. They are store large quantities of carbon in their biomass hence referred to as carbon sinks. This carbon can be emitted into the atmosphere when mangrove forests are degraded through unsustainable utility. The main objective of this study is to quantify the amount of carbon sequestered by the managed mangrove forest of the Mida Creek, Kenya. Three sites were selected for this study; Kibusa Plantation, Green Island Plantation, and a Natural Stand. Plots of (10 x10) m 2 were selected in each study site. Three carbon pools were investigated; aboveground carbon, belowground carbon, and soil organic carbon. Biomass for carbon determination in Kibusa and Natural Stands was estimated using a general equation. Mean total carbon stocks in Kibusa and Green Island Plantations was 424.52±11.68 Mg C/ha and 958.57±50.01 Mg C/ha while the natural stand contained significantly higher total Carbon stocks of 2159.77±31.09 Mg C/ha (ANOVA, F0.05(1),2,6 = 262.91, P < 0.05). There was no significant difference in the amount of soil organic carbon Int J Plant Anim Environ Sci 2021; 11 (2): 233-250 10.26502/ijpaes.202104 International Journal of Plant, Animal and Environmental Sciences Vol. 11 No. 2 – June 2021 234 among the three different sites (F0.05(1),2,15=0.35, p>0.05). This study indicates that reforestation enhances structural development of replanted mangroves and that replanted mangroves are significant carbon stores. From these results, we can deduce that awareness should be raised among the community members on the need for conservation and management which will increase the amount of carbon sequestered since more mangroves increase the rate of carbon (IV) oxide capture. This will help in mitigating the issue of global warming at local levels.
红树林在整个热带海岸的沿海环境中起着非常重要的作用。它们提供生态系统服务,能够维持在这些生态系统中发现的动植物群。它们在生物量中储存了大量的碳,因此被称为碳汇。当红树林因不可持续的利用而退化时,这些碳就会被排放到大气中。本研究的主要目标是量化肯尼亚米达溪管理红树林所吸收的碳量。本研究选择了三个地点;Kibusa种植园,绿岛种植园和一个自然林分。在每个研究地点选择(10 × 10) m2的地块。研究了三个碳库;地上碳,地下碳和土壤有机碳。利用一般方程估算了Kibusa林分和天然林分测定碳的生物量。Kibusa人工林和绿岛人工林的平均总碳储量分别为424.52±11.68 Mg C/ha和958.57±50.01 Mg C/ha,而自然林分的平均总碳储量为2159.77±31.09 Mg C/ha(方差分析,F0.05(1),2,6 = 262.91, P < 0.05)。土壤有机碳在不同地区间差异不显著[J] .植物与环境科学,2021;11 (2): 233-250 10.26502/ijpaes.202104国际植物,动物和环境科学杂志Vol. 11 No. 2 - June 2021 . 234在三个不同的地点(F0.05(1),2,15=0.35, p>0.05)。研究表明,再造林促进了再植红树林的结构发育,再植红树林具有重要的碳储量。根据这些结果,我们可以推断,应该提高社区成员对保护和管理的必要性的认识,这将增加固碳量,因为更多的红树林会增加碳(IV)氧化物的捕获率。这将有助于在地方层面上缓解全球变暖问题。
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引用次数: 0
Screening mungbean (Vigna radiata L.) lines for salinity tolerance using salinity induction response technique at seedling and physiological growth assay at whole plant level 利用苗期盐诱导响应技术和全株生理生长试验筛选绿豆耐盐品系
R. Manasa, Rameshraddy, H. Bindumadhava, R. Nair, T. G. Prasad, A. G. Shankar
Mungbean (Vigna radiata L.) is an economically important food legume rich in nutrients. However, its productivity over the last few decades has been stagnant largely due to various biotic and abiotic stresses. Among the abiotic stresses, salinity stress is more damaging that limits functional plant growth and yield worldwide. Due to the complex nature of salinity stress and lack of suitable techniques for introgression of desirable agronomic traits, little progress has been made in developing salt tolerant lines in legumes, in general and mungbean in particular. In the present investigation, an attempt has been made to screen mungbean lines for salinity tolerance. Forty mungbean lines were screened and contrasting lines were identified based on Salinity Induction Response (SIR) technique at the seedling level. As tolerance is a developmental stage specific, we further subjected the identified nine tolerant and nine susceptible lines for physiology based whole plant growth and yield phenotyping assay under 150 and 300 mM NaCl stress in pots. The results shown a considerable reduction in growth and yield performances of both tolerant and susceptible lines, but a few lines displayed relatively a better biomass and pod yield on par with non-stressed control plants. Based on seedling and whole plant level tolerance, a few tolerant (EC 693357, 58, 66, 71 and ML 1299) lines were identified for further investigation. Efforts are underway to use these identified tolerant lines as donor source for salinity breeding program to introgress with high yielding popular varieties.
绿豆(Vigna radiata L.)是一种营养丰富的经济重要食品豆类。然而,在过去的几十年里,由于各种生物和非生物的压力,其生产力一直停滞不前。在非生物胁迫中,盐度胁迫的危害更大,限制了植物的功能性生长和产量。由于盐胁迫的复杂性和缺乏适当的农艺性状渗入技术,在培育豆类,特别是绿豆耐盐品系方面进展甚微。在本研究中,对绿豆耐盐品系进行了筛选。采用盐诱导响应(SIR)技术筛选了40个绿豆品系,并在苗期鉴定了对照品系。由于耐受性是发育阶段特异性的,我们进一步将鉴定的9个耐受性和9个易感品系在150和300 mM NaCl胁迫下进行了盆栽生理全株生长和产量表型分析。结果表明,抗性和敏感品系的生长和产量表现均显著下降,但少数品系的生物量和荚果产量相对较好,与未受胁迫的对照植株相当。根据苗期和整株水平的耐受性,鉴定出EC 693357、58、66、71和ML 1299等耐受性品系。目前正在努力利用这些鉴定出的耐盐品系作为盐育种计划的供体来源,向高产的流行品种进行渗透。
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引用次数: 18
DISTRIBUTION AND CONCENTRATION SEVERAL TYPES OF HEAVY METAL CORRELATED WITH DIVERSITY AND ABUNDANCE OF MICROALGAE AT TALLO RIVER, MAKASSAR, SOUTH SULAWESI, INDONESIA 印度尼西亚南苏拉威西望加锡塔洛河几种重金属的分布和浓度与微藻多样性和丰度相关
Pub Date : 2015-03-25 DOI: 10.5376/ijms.2015.05.0022
N. Rukminasari, S. Sahabuddin
The main problem of aquatic ecosystem is caused by pollution of heavy metal that comes from industrial disposal and domestic. This pollution is causing loss of biodiversity, also increasing of bioaccumulation and biomagnifications from the poisonous materials at the food web. River ecosystem is the most affected by aquatic pollution due to a major role of the river for community. A study on water quality, heavy metal concentration and microalgae abundance at selected sites of Tallo River was carried out. Eighteen water and sediment samples were collected along the river for heavy metal and water quality analysis and 27 water samples were collected for microalgae analysis. The aims of this research are: 1) Determine distribution and concentration of existing heavy metal Tallo River ecosystem, 2) Identify microalgae dominant from Tallo River ecosystem where have been polluted by several type of heavy metal. The results showed that there were three heavy metals at Tallo River which have a high concentration and above permissible maximum concentration of environment quality standard, such as Lead (Pb), Copper (Cu) and Chromium (Cr). Those heavy metals were distributed patchily between station sampling. The highest concentrations of those heavy metals were found close to the industrial location of the Tallo River. Statistical result showed that there was a significant different of heavy metal concentration between sampling stations and concentration all of heavy metal measured was higher at sediment than in the water column. Only four genera found during sampling. Skeletonema sp and Synedra sp were abundance at all sites of the research location. However, there is a weak regression between abundance of microalgae, species dominant and concentration of heavy metal (Pb, Cu and Cd) in the sediment and water column.
水生生态系统的主要问题是工业处理和生活重金属的污染。这种污染正在造成生物多样性的丧失,也增加了食物网中有毒物质的生物积累和生物放大。河流生态系统受水体污染的影响最大,因为河流对社区起着重要作用。对塔罗河水质、重金属浓度和微藻丰度进行了研究。沿河采集了18个水体和沉积物样本进行重金属和水质分析,采集了27个水体样本进行微藻分析。本研究的目的是:1)确定塔罗河生态系统现有重金属的分布和浓度;2)确定塔罗河生态系统中已被几种重金属污染的微藻优势。结果表明,塔洛河水体中铅(Pb)、铜(Cu)和铬(Cr)三种重金属浓度均高于环境质量标准允许的最大浓度。这些重金属在各个采样站之间分布不均。这些重金属的最高浓度是在塔洛河工业区附近发现的。统计结果表明,各采样点间重金属含量存在显著差异,沉积物重金属含量均高于水柱重金属含量。在取样过程中只发现了4个属。研究点各样点均有丰富的骨灰体和蛇毒。微藻丰度、优势种与沉积物和水柱中重金属(Pb、Cu和Cd)浓度呈弱回归关系。
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引用次数: 1
REVIEW ON ANAEROBIC TREATMENT OF MUNICIPAL SOLID WASTE WITH LEACHATE RECIRCULATION 城市生活垃圾渗滤液循环厌氧处理研究进展
Pub Date : 2015-02-01 DOI: 10.1002/CHIN.201506317
Mansi Rastogi, R. Hooda, M. Nandal
Anaerobic bioreactor landfills are the most popular method to treat municipal solid waste as they rapidly stabilize waste, enhance leachate treatment along with increased landfill gas generation for cost-effective energy recovery and reduced post closure maintenance period. Leachate recirculation is one of the methods to apply in bioreactor to eliminate the leachate treatment and ground water contamination. The landfill leachate is characterized by high contents of organic and inorganic compounds which gradually decrease and depict the stabilization of municipal solid waste. Concentrations of these compounds in leachate depends on the type of waste, its susceptibility to dissolution, chemical and biological degradation and the volume of water running through the waste. The objective of this review paper is to highlight the effects of leachate recirculation on waste stabilization in a simulated bioreactor and to depict that the bioreactor efficiently stabilizes the waste mined from dumpsite by enhancing organic degradation
厌氧生物反应器填埋是最受欢迎的处理城市固体废物的方法,因为它可以快速稳定废物,加强渗滤液处理,同时增加垃圾填埋气的产生,以实现经济有效的能源回收,并缩短关闭后的维护周期。渗滤液回灌是应用于生物反应器中消除渗滤液处理和地下水污染的方法之一。垃圾渗滤液的特点是有机和无机化合物含量高,并逐渐减少,表现为城市生活垃圾的稳定化。这些化合物在渗滤液中的浓度取决于废物的类型、其对溶解、化学和生物降解的敏感性以及流经废物的水量。本文的目的是强调在模拟生物反应器中渗滤液循环对废物稳定化的影响,并描述生物反应器通过增强有机降解来有效地稳定垃圾场开采的废物
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引用次数: 11
MODELLING AND LIGAND INTERACTION STUDIES OF ENDO-1,4-BETA-XYLANASE FROM BACILLUS SUBTILIS 枯草芽孢杆菌内生-1,4- β -木聚糖酶的建模和配体相互作用研究
Pub Date : 2013-01-01 DOI: 10.13140/RG.2.2.30713.57442
Sreenath Konanki, J. Daddam, S. Anitha, M. Dowlathabad
Xylanase is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4- xylan into xylose, thus breaking down hemicellulose, which is a major component of the cell wall of plants. The sequence of Xylanase from Bacillus subtilis was obtained from NCBI. The predicted domain was searched to find out the related protein structure to be used as a template by the Basic Local Alignment Search Tool (BLAST) program against Protein Databank (PDB). Sequence that showed maximum identity with high score and less e-value was aligned and used as a reference structure to build a 3D model for Xylanase. In order to understand the mechanisms of ligand binding and the interaction between the ligand and the Xylanase a three-dimensional (3D) model of the Xylanase is generated based on the crystal structure of the Template by using the Modeller. With the aid of the molecular mechanics and molecular dynamics methods, the final refined model is obtained and is further assessed by Profile-3D, which shows that the refined model is reliable. With this model, a flexible docking study is performed with the acetate ion as ligand. After the docking studies, important determined residues in binding are identified. The hydrogen bonds play an important role for the stability of the complex. These results may be helpful for further experimental investigations.
木聚糖酶是一类酶的名称,它可以将线状多糖-1,4-木聚糖分解成木糖,从而分解半纤维素,而半纤维素是植物细胞壁的主要成分。从NCBI中获得枯草芽孢杆菌木聚糖酶的序列。利用BLAST (Basic Local Alignment Search Tool)程序对预测结构域进行检索,找出相关的蛋白结构作为模板。选取同源性最大、e值越高、e值越小的序列作为参考结构,建立木聚糖酶的三维模型。为了了解配体结合的机制以及配体与木聚糖酶之间的相互作用,利用modeler基于模板的晶体结构生成了木聚糖酶的三维(3D)模型。借助分子力学和分子动力学方法,得到了最终的精化模型,并通过Profile-3D进行了进一步的评估,结果表明精化模型是可靠的。利用该模型,以醋酸离子为配体进行了柔性对接研究。在对接研究之后,确定了结合中重要的确定残基。氢键对配合物的稳定性起着重要的作用。这些结果可能对进一步的实验研究有所帮助。
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引用次数: 1
EFFECT OF PESTICIDES ON MICROBIAL TRANSFORMATION OF SULPHUR IN SOIL 农药对土壤中硫微生物转化的影响
Pub Date : 2011-04-30 DOI: 10.5897/JSSEM.9000026
P. Giri, M. Saha, M. Halder, D. Mukherjee
A pot study was conducted in the laboratory of Department of Agricultural Chemistry and Soil Science, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, India in the year 2007-2008 to investigate the effect of pesticides, on the microbial transformation of sulphur (S) in soil. Insecticide (Endosulfan), fungicide (Dithane M-45), herbicide (2,4-D) were added to the soil at their recommended doses, respectively and their effect on the proliferation and potentiality of thiosulphate oxiding bacteria, aryl sulphatase, available and total sulphur were investigated in soil. The results of the present investigation revealed that insecticide, endosulfan effectuated a significant detrimental effect on some microbiological, biochemical and chemical properties in soil whereas fungicide, dithane M-45 caused a significant detrimental influence during the later stages in spite of stimulating influence at early stages incubation period. However, herbicide, 2,4-D, brought about a beneficial influence on the microbiological, biochemical and chemical properties in soil. Among the pesticides used in the study, the performance of the herbicide was favorable in all respect.
2007-2008年,在印度西孟加拉邦Nadia Mohanpur Bidhan Chandra Krishi Viswavidyalaya农业化学与土壤科学系实验室进行了一项盆栽研究,以调查农药对土壤中硫(S)微生物转化的影响。在土壤中按推荐剂量分别添加杀虫剂(硫丹)、杀菌剂(Dithane M-45)和除草剂(2,4- d),考察了它们对土壤中硫代硫酸盐氧化菌、芳基硫酸酶、有效硫和总硫的增殖和潜能的影响。本研究结果表明,杀虫剂硫丹对土壤中某些微生物、生化和化学性质产生了显著的不利影响,而杀菌剂乙烷M-45虽然在孵化初期具有刺激作用,但在后期产生了显著的不利影响。而2,4- d除草剂对土壤的微生物学、生化和化学性质都产生了有益的影响。在所使用的农药中,该除草剂在各方面的性能均较好。
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引用次数: 8
期刊
The International Journal of Plant, Animal and Environmental Sciences
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