{"title":"Rapid Detection of Pseudomonas aeruginosa Causing Ventilator Associated Pneumonia Using Multiple Cross Displacement Amplification Technique in Intensive Care Units in Tanta University Hospitals","authors":"Shaimaa Marey, Abd Alreheem Ads, Azza Hassan, Ghada Elbaradey, Radwa Eissa","doi":"10.21608/ejmm.2023.322729","DOIUrl":"https://doi.org/10.21608/ejmm.2023.322729","url":null,"abstract":"ABSTRACT","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136153145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/ejmm.2023.313257
R. E. El Sayed, Mysa S. Mostafa, A. Mahedy, Kawthar Mohamed
ABSTRACT
{"title":"Invasive Versus Noninvasive Methods for Diagnosis of Helicobacter Pylori infection","authors":"R. E. El Sayed, Mysa S. Mostafa, A. Mahedy, Kawthar Mohamed","doi":"10.21608/ejmm.2023.313257","DOIUrl":"https://doi.org/10.21608/ejmm.2023.313257","url":null,"abstract":"ABSTRACT","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89956725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/ejmm.2023.320674
Wafaa Elkasaby, Fikry El-Morsy, Mohammad El badrawy, Niveen El-wakeel
Background: Invasive fungal lung infection (IFLI) is a collective term used to describe a range of opportunistic infections, caused by one or more endemic or opportunistic fungi, the most isolated microbes in these infections are Candida species, Aspergillus species and Cryptococcus species. Clinical symptoms typically manifest as fever, cough, dyspnea, chest pain, and hemoptysis, Radiologic examination showed various presentations, few had typical features, such as denseness, cavitation, a halo sign some even showed negative results. For diagnosis; microscopy, fungal culture, galactomannan antigen, and PCR are useful tests. Other tools include imaging facilities and blood serum biomarkers. Antigen detection methods include (1-3)-β-D-Glucan lacks higher sensitivity, PCR hasn’t been standardized, in this study, we evaluated serum (1-3)-β-D- Glucan antigen in comparison with PCR for diagnosis accuracy. Objective: This study aimed to evaluate serum (1-3)-β-D-Glucan antigen detection and PCR methods for diagnosis of invasive fungal lung infections . Methodology: The study conducted a cross-sectional comparative study on suspected IFLI patients and a healthy control group. Clinical and radiological criteria were used for patient selection. Blood and bronchoalveolar lavage (BAL) samples were collected for biomarker assessment. PCR and BDG antigen detection were evaluated. Results: Male patients accounted for 78.1%, with no significant gender difference. Hypertension (40%), followed by diabetes (22%), were identified as the most common risk factors. BDG antigen levels were significantly associated with fungal infections. Sensitivity was 92%, specificity 80%, with an AUC of 0.879. BDG levels correlated with treatment outcomes. Conclusion: Elevated BDG levels were associated with cancer and COPD patients. While BDG provides supportive evidence for fungal infection, a multifaceted approach is crucial. Implementing new diagnostic tools and centralizing laboratories of fungal identification in clinical setting are essential as the diagnostic landscape evolves.
{"title":"(1-3)-β-D-Glucan Antigen Detection and PCR for Diagnosis of Invasive Fungal Lung Infections","authors":"Wafaa Elkasaby, Fikry El-Morsy, Mohammad El badrawy, Niveen El-wakeel","doi":"10.21608/ejmm.2023.320674","DOIUrl":"https://doi.org/10.21608/ejmm.2023.320674","url":null,"abstract":"Background: Invasive fungal lung infection (IFLI) is a collective term used to describe a range of opportunistic infections, caused by one or more endemic or opportunistic fungi, the most isolated microbes in these infections are Candida species, Aspergillus species and Cryptococcus species. Clinical symptoms typically manifest as fever, cough, dyspnea, chest pain, and hemoptysis, Radiologic examination showed various presentations, few had typical features, such as denseness, cavitation, a halo sign some even showed negative results. For diagnosis; microscopy, fungal culture, galactomannan antigen, and PCR are useful tests. Other tools include imaging facilities and blood serum biomarkers. Antigen detection methods include (1-3)-β-D-Glucan lacks higher sensitivity, PCR hasn’t been standardized, in this study, we evaluated serum (1-3)-β-D- Glucan antigen in comparison with PCR for diagnosis accuracy. Objective: This study aimed to evaluate serum (1-3)-β-D-Glucan antigen detection and PCR methods for diagnosis of invasive fungal lung infections . Methodology: The study conducted a cross-sectional comparative study on suspected IFLI patients and a healthy control group. Clinical and radiological criteria were used for patient selection. Blood and bronchoalveolar lavage (BAL) samples were collected for biomarker assessment. PCR and BDG antigen detection were evaluated. Results: Male patients accounted for 78.1%, with no significant gender difference. Hypertension (40%), followed by diabetes (22%), were identified as the most common risk factors. BDG antigen levels were significantly associated with fungal infections. Sensitivity was 92%, specificity 80%, with an AUC of 0.879. BDG levels correlated with treatment outcomes. Conclusion: Elevated BDG levels were associated with cancer and COPD patients. While BDG provides supportive evidence for fungal infection, a multifaceted approach is crucial. Implementing new diagnostic tools and centralizing laboratories of fungal identification in clinical setting are essential as the diagnostic landscape evolves.","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.21608/ejmm.2023.323674
Magdy Elsharkawy, Ahmed Emara, Abdelrahman Elbraky, Shaimaa Abdallah
ABSTRACT
{"title":"Kidney Transplantation with Modification of Induction Protocol with Infection Control Strategy during COVID-19 Era: Graft and Patients' Outcomes","authors":"Magdy Elsharkawy, Ahmed Emara, Abdelrahman Elbraky, Shaimaa Abdallah","doi":"10.21608/ejmm.2023.323674","DOIUrl":"https://doi.org/10.21608/ejmm.2023.323674","url":null,"abstract":"ABSTRACT","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136247329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-27DOI: 10.21608/ejm.2023.226021.1232
Feriala A. Abo Safe
{"title":"Evaluating the Safety Properties of Probiotic Lactobacillus spp. from Fermented Food in Egypt","authors":"Feriala A. Abo Safe","doi":"10.21608/ejm.2023.226021.1232","DOIUrl":"https://doi.org/10.21608/ejm.2023.226021.1232","url":null,"abstract":"","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135587683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-21DOI: 10.21608/ejm.2023.216815.1230
Doaa Abouzeid, Soha A. El-Hady, M. Abouzeid, N. Ibrahim
{"title":"Analysis of Species Distribution and Antifungal Susceptibilities among Locally Prevailed Clinical Isolates of Candida","authors":"Doaa Abouzeid, Soha A. El-Hady, M. Abouzeid, N. Ibrahim","doi":"10.21608/ejm.2023.216815.1230","DOIUrl":"https://doi.org/10.21608/ejm.2023.216815.1230","url":null,"abstract":"","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85728810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.21608/ejm.2023.207321.1228
B. Sherif, Yasmeen Mahmood
{"title":"Invasive Candidiasis: Prevalence, Species Distribution and Trends in Antifungal Susceptibility in an Egyptian University Hospital","authors":"B. Sherif, Yasmeen Mahmood","doi":"10.21608/ejm.2023.207321.1228","DOIUrl":"https://doi.org/10.21608/ejm.2023.207321.1228","url":null,"abstract":"","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82438026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/ejmm.2023.312063
I. Afifi, Asmaa S Mostafa, Waseem Hassan, S. AlShareef, Sherhana Abdulmajid, E. Elsebaei
Background: Blood culture contamination is a global problem that heeds all healthcare settings and has many negative impacts. Objectives: to compare the effectiveness of the two implemented interventions on blood culture contamination rates. Methodology: The blood culture reports of specimens received by the microbiology laboratory during the study period were grouped into 3 groups; pre-intervention group, post-intervention I group after educational lectures and practical workshops, post-intervention II group after implementation of the same intervention I together with individual counseling for staff identified as having obtained contaminated specimens. The contamination rates were evaluated and compared to the target and as regards departments and organisms. Results: After intervention I, there was a 31.56% reduction rate while after post-intervention II there was a 56.8% reduction from the pre-intervention rate. The total number of contaminants showed a highly significant difference between pre-intervention and post-intervention I & between post-interventions I and II (p=0.001) and an extremely highly significant difference between pre-intervention and post-intervention II (p=0.0001). The highest rate of contamination was found in the emergency department followed by ICUs. The contaminants were coagulase-negative staphylococci (CoNS) (82.8%, 92.6%, 93.3%) micrococci (9.7%, 5.5% 6.7%), anthracoid (4.9%,1.2%, 0%) and Corynebacterium spp. (2.6%, 0.6%, 0%) in the three groups pre or post interventions respectively. Conclusion: Intervention II proved to be more effective in reducing blood culture contamination rate. So, it is recommended to continuously track the contamination rate and train the staff on the best practice together with disciplinary counseling for those who frequently withdraw contaminated blood culture specimens.
{"title":"The Impact of Performance Improvement Interventions and Phlebotomy Staff Counseling on Blood Culture Contamination Rates: Experience of Security Forces Hospital, Makkah, Saudi Arabia","authors":"I. Afifi, Asmaa S Mostafa, Waseem Hassan, S. AlShareef, Sherhana Abdulmajid, E. Elsebaei","doi":"10.21608/ejmm.2023.312063","DOIUrl":"https://doi.org/10.21608/ejmm.2023.312063","url":null,"abstract":"Background: Blood culture contamination is a global problem that heeds all healthcare settings and has many negative impacts. Objectives: to compare the effectiveness of the two implemented interventions on blood culture contamination rates. Methodology: The blood culture reports of specimens received by the microbiology laboratory during the study period were grouped into 3 groups; pre-intervention group, post-intervention I group after educational lectures and practical workshops, post-intervention II group after implementation of the same intervention I together with individual counseling for staff identified as having obtained contaminated specimens. The contamination rates were evaluated and compared to the target and as regards departments and organisms. Results: After intervention I, there was a 31.56% reduction rate while after post-intervention II there was a 56.8% reduction from the pre-intervention rate. The total number of contaminants showed a highly significant difference between pre-intervention and post-intervention I & between post-interventions I and II (p=0.001) and an extremely highly significant difference between pre-intervention and post-intervention II (p=0.0001). The highest rate of contamination was found in the emergency department followed by ICUs. The contaminants were coagulase-negative staphylococci (CoNS) (82.8%, 92.6%, 93.3%) micrococci (9.7%, 5.5% 6.7%), anthracoid (4.9%,1.2%, 0%) and Corynebacterium spp. (2.6%, 0.6%, 0%) in the three groups pre or post interventions respectively. Conclusion: Intervention II proved to be more effective in reducing blood culture contamination rate. So, it is recommended to continuously track the contamination rate and train the staff on the best practice together with disciplinary counseling for those who frequently withdraw contaminated blood culture specimens.","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75870547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/ejmm.2023.305231
N. Radi, Alzahraa Fahmy, Taha Singer, M. Abdel-Aziz
ABSTRACT
摘要
{"title":"The Correlation between Ciprofloxacin Resistant Salmonella Strains and Its Ability to Biofilm Formation","authors":"N. Radi, Alzahraa Fahmy, Taha Singer, M. Abdel-Aziz","doi":"10.21608/ejmm.2023.305231","DOIUrl":"https://doi.org/10.21608/ejmm.2023.305231","url":null,"abstract":"ABSTRACT","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78834280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-01DOI: 10.21608/ejmm.2023.312957
Areej M El-Mahdy, Y. Mahran
ABSTRACT
摘要
{"title":"The Impact of Staphylococcus aureus on Biofilm, Antibiotic Resistance and Cytotoxicity of Pseudomonas aeruginosa Isolated from Mixed Wound Infections","authors":"Areej M El-Mahdy, Y. Mahran","doi":"10.21608/ejmm.2023.312957","DOIUrl":"https://doi.org/10.21608/ejmm.2023.312957","url":null,"abstract":"ABSTRACT","PeriodicalId":22549,"journal":{"name":"The Egyptian Journal of Medical Microbiology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88720679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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