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EFFECT OF MUTAGENS ON BIOMASS AND LIPID CONTENT OF ULOTHRIX sp. AND OSCILLATORIA sp. FOR IMPROVED BIODIESEL production 诱变剂对乌氏菌和振荡菌生物量和脂质含量的影响
Pub Date : 2022-09-20 DOI: 10.36899/japs.2023.1.0606
N. Munir, M. Samad, N. Sharif, R. Haq, S. Naz
Chemical and physical mutagens like sodium azide, H 2 O 2 and UV light have a significant role in the metabolism of living organisms. Effect of these mutagens was recorded during the present work on lipid content of Oscillatoria sp . and Ulothrix sp. The algal species were subjected to the mutagens by adding them in culture media. Lipids were extracted by Microwave-assisted extraction using methanol, ethanol and chloroform. During the present work generally a significant effect of algal species and mutagens was observed. All the tested mutagen significantly affected the lipid content. A significant effect of solvent on lipid content of algal species was observed when the algae were extracted with different solvents after treatment with H 2 O 2 and UV light however this effect was non-significant when algae were subjected to sodium azide. . In general, the mutagen resulted in an increase in lipid content. In Oscillatoria sp. the lipid content was generally less as compared to Ulothrix sp. The highest lipid content of 0.5433±0.002 g100g -1 was recorded in Ulothrix sp. after 3 days stress of sodium azide stress followed by H 2 O 2 treated (50mM) samples with a content of 0.5133±0.05g100g -1 . In Oscillatoria sp. 15mM treatment for 3 days gave a lipid content of 0.3733±0.0001g100g -1 after 3 days. A reduction in fresh weights of both tested algal species was recorded after UV stress. The oil content also showed a significant reduction after UV exposure. GC-MS analysis of the transesterified lipids showed that different fatty acids show variation when algal samples were treated with mutagens. Hence this study is helpful in manipulation of algal oil for biodiesel production.
叠氮化钠、h2o2和紫外线等化学和物理诱变剂在生物体的代谢中起着重要作用。本研究记录了这些诱变剂对振荡藻脂质含量的影响。在培养基中加入诱变剂对藻类进行诱变。采用甲醇、乙醇和氯仿微波辅助萃取法提取脂质。在目前的工作中,一般观察到藻类和诱变剂的显著影响。所有被试诱变剂均显著影响脂质含量。用不同溶剂提取海藻,经过氧化氢和紫外光处理后,溶剂对藻类脂质含量有显著影响,而叠氮化钠对藻类脂质含量的影响不显著。总的来说,诱变剂导致脂质含量增加。在叠氮化钠胁迫3 d后,振荡藻脂质含量最高,为0.5433±0.002 g100g -1,其次是h2o2处理(50mM)样品,其脂质含量为0.5133±0.05g100g -1。15mM处理3天后,脂质含量为0.3733±0.0001g100g -1。在紫外线胁迫后,两种测试藻类的新鲜重量都有所减少。紫外线照射后,油含量也显著降低。气相色谱-质谱分析表明,在诱变剂作用下,不同脂肪酸发生了变化。因此,本研究对藻类油的制备具有一定的指导意义。
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引用次数: 0
PHYSIO-BIOLOGICAL MARKERS IN ZINC-INDUCED MOULTED AND NON-MOULTED WHITE LEGHORN LAYERS (GALLUS DOMESTICUS) 锌诱导脱毛和未脱毛白来角蛋鸡(家鸡)的生理生物学标记
Pub Date : 2022-09-20 DOI: 10.36899/japs.2023.1.0594
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引用次数: 0
IDENTIFICATION AND CHARACTERIZATION OF DIFFERENT POTENTIALLY ANTIBACTERIAL COMPOUNDS FROM A MARINE STREPTOMYCES SP. SP1 海洋链霉菌(Streptomyces sp. sp1)中不同潜在抗菌化合物的鉴定与特性研究
Pub Date : 2022-09-20 DOI: 10.36899/japs.2023.1.0605
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引用次数: 0
SUSTAINABILITY OF EXTENSIVE SHEEP FARMING PRACTICES: PASTORALISM AND TRADITIONAL USE OF MEDICINAL PLANTS, CASE STUDY ON THE MĂRGINIMEA SIBIULUI AREA, ROMANIA 广泛牧羊做法的可持续性:畜牧业和药用植物的传统利用,对罗马尼亚mĂrginimea sibiului地区的案例研究
Pub Date : 2022-09-20 DOI: 10.36899/japs.2023.1.0592
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引用次数: 1
SYNERGISTIC AND ANTAGONISTIC EFFECTS ON ANTIMICROBIAL PROPERTIES OF ORGANIC AND AQUEOUS EXTRACTS OF BACILLUS CLAUSII KP10 IN COMBINATION WITH CONVENTIONAL ANTIBIOTICS 克氏芽孢杆菌kp10有机和水提物与常规抗生素联合抑菌性能的增效和拮抗作用
Pub Date : 2022-09-20 DOI: 10.36899/japs.2023.1.0603
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引用次数: 0
EFFECTS OF DIET ON CCL11 EXPRESSION AND AGING OF LUNGS AND TESTES OF MICE 饮食对小鼠肺、睾丸ccl11表达及衰老的影响
Pub Date : 2022-08-20 DOI: 10.36899/japs.2023.1.0593
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引用次数: 0
COMPARATIVE EVALUATION OF PHYTOCHEMICAL COMPOSITION AND ANTIOXIDANT POTENTIAL OF SOME MEDICINALLY-IMPORTANT EUPHORBIACEOUS PLANTS 几种药用大戟属植物化学成分及抗氧化活性的比较评价
Pub Date : 2022-07-06 DOI: 10.36899/japs.2022.6.0578
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引用次数: 2
ASSESSING THE IMPACT OF TRAINING PROGRAMMES FOR DAIRY FARMERS BY KRISHI VIGYAN KENDRA, FARM SCIENCE CENTRE, INDIA 印度农业科学中心krishi vigyan kendra对奶农培训项目影响的评估
Pub Date : 2022-07-06 DOI: 10.36899/japs.2022.6.0566
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引用次数: 0
FEEDING BEHAVIOR AND FECUNDITY RATE OF SHRIMP SCAD ALEPES DJEDABA ALONG BALOCHISTAN COAST, PAKISTAN 巴基斯坦俾路支省沿海吉达巴虾的摄食行为和繁殖率
Pub Date : 2022-06-11 DOI: 10.36899/japs.2022.6.0584
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引用次数: 0
HEMATOLOGICAL PROFILE OF MARECHA CAMEL (CAMELUS DROMEDARIUS) REARED UNDER SEMI-INTENSIVE MANAGEMENT SYSTEM 半集约化管理下的骆驼血液学特征
Pub Date : 2022-06-11 DOI: 10.36899/japs.2022.6.0568
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引用次数: 0
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The Journal of Animal and Plant Sciences
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