Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0699
M. Ozden, R. Qaderi
International fresh table grape trade has expanded tremendously over the last few decades. However, fresh table grapes decay quickly due to pathogen infestation and water loss, which make it difficult to preserve without an effective treatment. Therefore, this study was conducted to investigate the effects of CT, with SA on maintaining quality characteristics of ‘Red Globe’ grapes during storage at 0.5°C and 90-95% RH in pomology laboratories of the faculty in 2018-2019. The experiment was conducted using a completely randomized design with treatments [1% CT and two concentrations of SA (0, 1 and 2 mM)] fora 60 day of storage time. To determine the effects of the treatmentsat 15-day intervals, weight loss, fruit decay, chroma index, total soluble solids, titratable acidity (TA), pH, fruit flesh firmness and secondary metabolites such as total phenolic contents, total flavonoids and total anthocyanins were determined. Also, antioxidant activity and antioxidant capacity of the berry extracts determined by the DPPH and FRAP assays at each sampling time. Results revealed no effect on total soluble solids and total phenolics However, there were significant effects on the quality parameters fruit decay, weight loss, TA, pH, chroma index, and fruit flesh firmness. Chitosan coating was also found to be a potentially useful treatment for the phytochemical compounds and total antioxidant activity. The pre-storage IC 50 value of the berry extract was 0.53 mg mL -1 and by the end of storage period, the values were 0.49 mg mL -1 in control, 0.50 mg mL -1 in CT, 0.53 mg mL -1 in CT + 1 mM SA and 0.46 mg mL -1 in CT + 2 mM SA. Total antioxidant capacity of grapes was 23.7 mg BHT mL -1 before storage and this increased during storage for both coated and uncoated grapes. Based on the findings, there was a strong relationship between total phenolic and flavonoid contents of the berries and antioxidant activity. It was concluded that the use of CT + 2 mM SA together with MAP is a potentially useful alternative to the use of SO 2 in grape storage in commercial contexts.
在过去的几十年里，国际鲜食葡萄贸易得到了极大的发展。然而，新鲜的鲜食葡萄由于病原体侵袭和水分流失而迅速腐烂，如果没有有效的处理，就很难保存。因此，本研究旨在研究2018-2019年在学院果学实验室0.5°C和90-95% RH条件下，CT加SA对保持“红地球”葡萄品质特性的影响。试验采用完全随机设计，处理[1% CT和两种浓度的SA(0,1和2 mM)]，储存时间为60天。为了确定每隔15天处理的效果，测定了失重、果实腐烂、色度指数、总可溶性固形物、可滴定酸度(TA)、pH、果肉硬度和次生代谢产物如总酚含量、总黄酮和总花青素含量。同时，通过DPPH和FRAP测定各采样时间的抗氧化活性和抗氧化能力。结果表明，总可溶性固形物和总酚类物质对果实腐烂、失重、TA、pH、色度指数和果肉硬度等品质参数均无显著影响。壳聚糖包被还被发现是一个潜在的有用的处理植物化学化合物和总抗氧化活性。贮藏结束时，对照组的ic50值为0.49 mg mL -1, CT组为0.50 mg mL -1, CT + 1 mM SA组为0.53 mg mL -1, CT + 2 mM SA组为0.46 mg mL -1。葡萄在贮藏前的总抗氧化能力为23.7 mg BHT mL -1，在贮藏过程中，包衣和未包衣葡萄的总抗氧化能力均有所增加。基于这些发现，浆果的总酚和类黄酮含量与抗氧化活性之间存在很强的关系。综上所述，在商业环境下，使用CT + 2mm SA和MAP是一种潜在的有用的替代so2在葡萄储存中的使用。
{"title":"COATING OF CHITOSAN AND SALICYLIC ACID CAN MAINTAIN QUALITY CHARACTERISTICS OF TABLE GRAPES","authors":"M. Ozden, R. Qaderi","doi":"10.36899/japs.2023.5.0699","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0699","url":null,"abstract":"International fresh table grape trade has expanded tremendously over the last few decades. However, fresh table grapes decay quickly due to pathogen infestation and water loss, which make it difficult to preserve without an effective treatment. Therefore, this study was conducted to investigate the effects of CT, with SA on maintaining quality characteristics of ‘Red Globe’ grapes during storage at 0.5°C and 90-95% RH in pomology laboratories of the faculty in 2018-2019. The experiment was conducted using a completely randomized design with treatments [1% CT and two concentrations of SA (0, 1 and 2 mM)] fora 60 day of storage time. To determine the effects of the treatmentsat 15-day intervals, weight loss, fruit decay, chroma index, total soluble solids, titratable acidity (TA), pH, fruit flesh firmness and secondary metabolites such as total phenolic contents, total flavonoids and total anthocyanins were determined. Also, antioxidant activity and antioxidant capacity of the berry extracts determined by the DPPH and FRAP assays at each sampling time. Results revealed no effect on total soluble solids and total phenolics However, there were significant effects on the quality parameters fruit decay, weight loss, TA, pH, chroma index, and fruit flesh firmness. Chitosan coating was also found to be a potentially useful treatment for the phytochemical compounds and total antioxidant activity. The pre-storage IC 50 value of the berry extract was 0.53 mg mL -1 and by the end of storage period, the values were 0.49 mg mL -1 in control, 0.50 mg mL -1 in CT, 0.53 mg mL -1 in CT + 1 mM SA and 0.46 mg mL -1 in CT + 2 mM SA. Total antioxidant capacity of grapes was 23.7 mg BHT mL -1 before storage and this increased during storage for both coated and uncoated grapes. Based on the findings, there was a strong relationship between total phenolic and flavonoid contents of the berries and antioxidant activity. It was concluded that the use of CT + 2 mM SA together with MAP is a potentially useful alternative to the use of SO 2 in grape storage in commercial contexts.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81440426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0704
G. Sunkad, S. Meghana, S. T. Yenjerappa, S. Kulkarni, S. Rao, N. Naik
Dry root rot is a major threat to chickpea production globally. The disease is caused by Rhizoctonia bataticola. The recent rise in global temperature and worsening of drought spells has aggravated dry root rot outbreaks in chickpeas. To combat this necrotrophic pathogen, the present study was conducted to inhibit the pathogen by using potential plant growth-promoting microorganisms (PGPMs). Forty isolates of endophytic fungal PGPMs were isolated from healthy plant parts of chickpeas in a potato dextrose agar medium. Isolates were screened for antagonistic potential and bioactive compound production against R. bataticola. Using ITS genes BLAST analysis, the putative endophytic fungal PGPM was identified at the molecular level. Finally, the extraction of bio-active compounds and metabolic profiling was carried out by solvent extraction method and GC-MS/MS technique, respectively. Among forty isolates, FEPGPM-34 produced the maximum concentration of bio-active compounds with the highest percent mycelial inhibition of 74.61. Through molecular characterization and BLAST analysis, the isolateFEPGPM-34 was identified as Trichoderma asperellum . GC-MS/MS analysis of T. asperellum extract showed the presence of 65 compounds at different retention times and mass to charge (m/z) ratios with 13 compounds exhibiting antimicrobial properties. Hence, PGPMs can be exploited for managing dry root rot disease.
{"title":"TRICHODERMA ASPERELLUM: A TREASURE HOUSE OF BIOACTIVE COMPOUNDS WITH ANTAGONISTIC ACTIVITY AGAINST Rhizoctonia bataticola, A CAUSAL AGENT OF DRY ROOT ROT IN CHICKPEA","authors":"G. Sunkad, S. Meghana, S. T. Yenjerappa, S. Kulkarni, S. Rao, N. Naik","doi":"10.36899/japs.2023.5.0704","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0704","url":null,"abstract":"Dry root rot is a major threat to chickpea production globally. The disease is caused by Rhizoctonia bataticola. The recent rise in global temperature and worsening of drought spells has aggravated dry root rot outbreaks in chickpeas. To combat this necrotrophic pathogen, the present study was conducted to inhibit the pathogen by using potential plant growth-promoting microorganisms (PGPMs). Forty isolates of endophytic fungal PGPMs were isolated from healthy plant parts of chickpeas in a potato dextrose agar medium. Isolates were screened for antagonistic potential and bioactive compound production against R. bataticola. Using ITS genes BLAST analysis, the putative endophytic fungal PGPM was identified at the molecular level. Finally, the extraction of bio-active compounds and metabolic profiling was carried out by solvent extraction method and GC-MS/MS technique, respectively. Among forty isolates, FEPGPM-34 produced the maximum concentration of bio-active compounds with the highest percent mycelial inhibition of 74.61. Through molecular characterization and BLAST analysis, the isolateFEPGPM-34 was identified as Trichoderma asperellum . GC-MS/MS analysis of T. asperellum extract showed the presence of 65 compounds at different retention times and mass to charge (m/z) ratios with 13 compounds exhibiting antimicrobial properties. Hence, PGPMs can be exploited for managing dry root rot disease.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74244876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0698
B. Erdal, Ş. Turhan, P. A. Temel
The aim of this paper is to identify factors influencing the purchase intention of generations towards organic products in Bursa, Türkiye. The study was conducted with a quantitative approach with data collection from 395 respondents in Bursa City between November 2021 and February 2022. The results were investigated using descriptive analysis, reliability analysis, exploratory factor analysis, correlation analysis, chi-square analysis and regression analysis. It is important to consider generational characteristics in order to achieve realistic results in organic product consumption studies. Thus, marketers will be provided with a different perspective and they will develop more consistent marketing strategies. In this research, it has been determined that the reasons for buying a product differ in the context of generations. According to the results, especially the consumers in the Y Generation are more interested in the concept of organic products. It is seen that social media is the most effective (43%) source of organic product information. According to the results of the factor analysis, the environmental friendliness of organic products and the high nutritional value of organic products are the first preferences of consumers. The least important variable is the reliability of organic products. Within the scope of the research, the organic food product trends of 5 generations were discussed. According to the results obtained, it has been determined that the tendency of the Z Generation towards organic food is lower than that of the X and Y Generations. It can be thought that this situation is due to the fact that they are young and do not have enough economic power yet. It is clearly seen that generational characteristics affect the consumption of organic products. This result will help to identify the problems and opportunities in the organic product market. Raising awareness, informing and directing individuals towards organic products in a way that covers all generations will be an important step in terms of both the country’s economy and the healthy functioning of the ecosystem.
{"title":"GENERATIONAL DIFFERENCES TOWARD ORGANIC FOOD BEHAVIOR","authors":"B. Erdal, Ş. Turhan, P. A. Temel","doi":"10.36899/japs.2023.5.0698","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0698","url":null,"abstract":"The aim of this paper is to identify factors influencing the purchase intention of generations towards organic products in Bursa, Türkiye. The study was conducted with a quantitative approach with data collection from 395 respondents in Bursa City between November 2021 and February 2022. The results were investigated using descriptive analysis, reliability analysis, exploratory factor analysis, correlation analysis, chi-square analysis and regression analysis. It is important to consider generational characteristics in order to achieve realistic results in organic product consumption studies. Thus, marketers will be provided with a different perspective and they will develop more consistent marketing strategies. In this research, it has been determined that the reasons for buying a product differ in the context of generations. According to the results, especially the consumers in the Y Generation are more interested in the concept of organic products. It is seen that social media is the most effective (43%) source of organic product information. According to the results of the factor analysis, the environmental friendliness of organic products and the high nutritional value of organic products are the first preferences of consumers. The least important variable is the reliability of organic products. Within the scope of the research, the organic food product trends of 5 generations were discussed. According to the results obtained, it has been determined that the tendency of the Z Generation towards organic food is lower than that of the X and Y Generations. It can be thought that this situation is due to the fact that they are young and do not have enough economic power yet. It is clearly seen that generational characteristics affect the consumption of organic products. This result will help to identify the problems and opportunities in the organic product market. Raising awareness, informing and directing individuals towards organic products in a way that covers all generations will be an important step in terms of both the country’s economy and the healthy functioning of the ecosystem.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83213227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0710
H. Altaweel, F. Al‐Daoud, G. Mushtaq
Cryptosporidium is one of the most common causes of diarrhea in calves worldwide, and the risk factors contributing to its control and prevention are extensively studied. This epidemiological study was conducted in an unstable breeding environment. Ninety-six (96) calves suffering from diarrhea were studied from five regions in northwestern Syria. The goal of this study was to assess the prevalence of cryptosporidium in fecal samples and to measure the expected risk and regression for assumed risk factors that may cause diarrhea in calves up to 60 days old. Fecal samples were tested using direct ELISA. The results showed 9.1% (1/11) cases in the western Aleppo countryside, 21.1% (4/19) in the eastern Idlib countryside, 13.6% (3/22) in the Northern Idlib countryside, 2.9% (1/35) in the Jisr Al-Shughur countryside, while no positive case was recorded in the western countryside. This is the first study conducted in the region showing the incidence, regression, and risk rates, and it accentuates the need for a broader study on the effect of certain drugs used in the treatment of diarrhea and its consequences.
{"title":"STUDY ON THE PREVALENCE OF CRYPTOSPORIDIUM IN CALVES SUFFERING FROM DIARRHEA IN NORTHWESTERN SYRIA","authors":"H. Altaweel, F. Al‐Daoud, G. Mushtaq","doi":"10.36899/japs.2023.5.0710","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0710","url":null,"abstract":"Cryptosporidium is one of the most common causes of diarrhea in calves worldwide, and the risk factors contributing to its control and prevention are extensively studied. This epidemiological study was conducted in an unstable breeding environment. Ninety-six (96) calves suffering from diarrhea were studied from five regions in northwestern Syria. The goal of this study was to assess the prevalence of cryptosporidium in fecal samples and to measure the expected risk and regression for assumed risk factors that may cause diarrhea in calves up to 60 days old. Fecal samples were tested using direct ELISA. The results showed 9.1% (1/11) cases in the western Aleppo countryside, 21.1% (4/19) in the eastern Idlib countryside, 13.6% (3/22) in the Northern Idlib countryside, 2.9% (1/35) in the Jisr Al-Shughur countryside, while no positive case was recorded in the western countryside. This is the first study conducted in the region showing the incidence, regression, and risk rates, and it accentuates the need for a broader study on the effect of certain drugs used in the treatment of diarrhea and its consequences.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88226503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0713
S. Kanwal, R. Qamar, S. Jamil, M. Tahir
Genetic variation and varied response of genotypes with erraticsalt tolerance enable us to recognize and utilize the underlying physiological and biochemical mechanisms in development of salinity tolerant cultivars. In the present studies, ten lines viz., B-56, BLBN, G-96, R-3, RBJ-8007, ZMR-1, ZMR-2, ZNR-1, ZMM-5, and 23627, and six testers i.e., Faisal, Shiralee, Laclone, Long, Legend, and ZMR-3 were collected and crossed in a line by tester fashion. The resultant F 1 crosses and their parental genotypes were assessed at maturity phase in a pot experiment under factorial structured CRD. Recorded data on morphological traits were analyzed to assess the genetic variability in breeding material, association among traits, and their direct and subsidiary effects on seed and oil yield. Analysis of variance showed the presence of significant genetic variability within and among the salinity treatments. Parental genotypes were found significantly different for Na+/K+ ratio, proline contents, osmotic potential, primary branches, and oil contents under all treatments. Entries and parents vs. crosses differed significantly for most of the traits indicating that breeding material possesses genetic variability which may be exploited in future breeding program for effective upgradation of yield and salinity tolerance. Correlation and path analysis exposed that silique per plant, seeds per silique, oil content, thousand seeds weight, and days to 50% maturity might be used as criteria of selection for development of salinity tolerant types with higher yield. Morphological traits showed significant association with yield per plant under salt stress conditions. These associations can be useful in sorting salt tolerant and sensitive high yielding genotypes. Accumulation of proline and Na + /K + ratio may also be chosen as selection criteria for targeting salinity tolerance in the future breeding program. Screening of salt tolerant lines and subsequent development of salinity tolerant cultivars may be helpful in inclusion of salt effected area’s under Brassica cultivation thereby increasing the local production of edible oil in Pakistan.
{"title":"EVALUATION AND SELECTION OF SALINITY TOLERANT LINES IN BRASSICA NAPUS USING CORRELATION AND PATH COEFFICIENT ANALYSIS","authors":"S. Kanwal, R. Qamar, S. Jamil, M. Tahir","doi":"10.36899/japs.2023.5.0713","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0713","url":null,"abstract":"Genetic variation and varied response of genotypes with erraticsalt tolerance enable us to recognize and utilize the underlying physiological and biochemical mechanisms in development of salinity tolerant cultivars. In the present studies, ten lines viz., B-56, BLBN, G-96, R-3, RBJ-8007, ZMR-1, ZMR-2, ZNR-1, ZMM-5, and 23627, and six testers i.e., Faisal, Shiralee, Laclone, Long, Legend, and ZMR-3 were collected and crossed in a line by tester fashion. The resultant F 1 crosses and their parental genotypes were assessed at maturity phase in a pot experiment under factorial structured CRD. Recorded data on morphological traits were analyzed to assess the genetic variability in breeding material, association among traits, and their direct and subsidiary effects on seed and oil yield. Analysis of variance showed the presence of significant genetic variability within and among the salinity treatments. Parental genotypes were found significantly different for Na+/K+ ratio, proline contents, osmotic potential, primary branches, and oil contents under all treatments. Entries and parents vs. crosses differed significantly for most of the traits indicating that breeding material possesses genetic variability which may be exploited in future breeding program for effective upgradation of yield and salinity tolerance. Correlation and path analysis exposed that silique per plant, seeds per silique, oil content, thousand seeds weight, and days to 50% maturity might be used as criteria of selection for development of salinity tolerant types with higher yield. Morphological traits showed significant association with yield per plant under salt stress conditions. These associations can be useful in sorting salt tolerant and sensitive high yielding genotypes. Accumulation of proline and Na + /K + ratio may also be chosen as selection criteria for targeting salinity tolerance in the future breeding program. Screening of salt tolerant lines and subsequent development of salinity tolerant cultivars may be helpful in inclusion of salt effected area’s under Brassica cultivation thereby increasing the local production of edible oil in Pakistan.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88880396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0708
T. B. Prakarsa, S. Hadisusanto, S. Pudyatmoko, I. Maryanto
Bats that feed on insects and are members of the Hipposideridae family, category Chiroptera is widely dispersed worldwide, including Hipposideros spp. There are 29 species of 73 species that inhabit Indonesia. Most of these species use caves as their roosting habitat. In cave habitats, with different physicochemical conditions from surface habitats, the hematological profile can be a physiological indicator in responding to habitat conditions. This study aims to analyze the hematological profile of Hipposideros spp. as a parameter of physiological adaptation in the cave habitat in Gunung Sewu Karst Area, Indonesia. Sampling was carried out purposively in six caves locality of the Gunung Sewu karst area. Bats were collected with misnet (12 x 3 m), and Harpnet was installed at the entrance of the cave at 17.00 - 21.00 WIB. The Blood samples from each bat were taken intravenously, as much as 1 mL, on the Forearm and inserted into a 1.5 mL microtube that had been given EDTA anticoagulant. A total of 20 parameters were observed from erythrocyte, leukocyte, and platelet profiles. These parameters use a Hematology Analyzer for analysis. Data differences in hematological profiles between species and sex were analyzed using one-way ANOVA (sig= 95%), respectively. The relationship trend between the physicochemical parameters of the roosting area in the cave with erythrocytes and hemoglobin was then analyzed using a univariate linear model with multiple regression. All statistical analyses were performed using the Paleontological statistics program (PAST) ver. 4.09 and R. Studio v1.4.1717-3. We found three species ( Hipposideros diadema, H. larvatus, and H. ater ) with 70 individuals. This study concluded that there were differences in the hematological profiles between the three Hipposideros species based on species and gender, although some samples did not show significant differences based on statistical tests. Erythrocytes and hemoglobin can be used as parameters of physiological adaptation to cave habitats. This is indicated by an increase in erythrocytes and hemoglobin followed by a decrease in air temperature and oxygen levels, as well as increased humidity and ammonia levels in cave habitats. These study results can be supported if similar studies analyze the hematological profile of non-cave-roosting bat. It is recommended that future studies analyze the hematological profile of non-cave-roosting bats around the caves of the studies or in areas with similar conditions.
{"title":"HEMATOLOGICAL PROFILE OF THREE SPECIES OF Hipposideros spp. (HIPPOSIDERIDAE) AS AN ADAPTATION IN CAVE HABITAT, IN GUNUNG SEWU GEOPARK AREA, INDONESIA","authors":"T. B. Prakarsa, S. Hadisusanto, S. Pudyatmoko, I. Maryanto","doi":"10.36899/japs.2023.5.0708","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0708","url":null,"abstract":"Bats that feed on insects and are members of the Hipposideridae family, category Chiroptera is widely dispersed worldwide, including Hipposideros spp. There are 29 species of 73 species that inhabit Indonesia. Most of these species use caves as their roosting habitat. In cave habitats, with different physicochemical conditions from surface habitats, the hematological profile can be a physiological indicator in responding to habitat conditions. This study aims to analyze the hematological profile of Hipposideros spp. as a parameter of physiological adaptation in the cave habitat in Gunung Sewu Karst Area, Indonesia. Sampling was carried out purposively in six caves locality of the Gunung Sewu karst area. Bats were collected with misnet (12 x 3 m), and Harpnet was installed at the entrance of the cave at 17.00 - 21.00 WIB. The Blood samples from each bat were taken intravenously, as much as 1 mL, on the Forearm and inserted into a 1.5 mL microtube that had been given EDTA anticoagulant. A total of 20 parameters were observed from erythrocyte, leukocyte, and platelet profiles. These parameters use a Hematology Analyzer for analysis. Data differences in hematological profiles between species and sex were analyzed using one-way ANOVA (sig= 95%), respectively. The relationship trend between the physicochemical parameters of the roosting area in the cave with erythrocytes and hemoglobin was then analyzed using a univariate linear model with multiple regression. All statistical analyses were performed using the Paleontological statistics program (PAST) ver. 4.09 and R. Studio v1.4.1717-3. We found three species ( Hipposideros diadema, H. larvatus, and H. ater ) with 70 individuals. This study concluded that there were differences in the hematological profiles between the three Hipposideros species based on species and gender, although some samples did not show significant differences based on statistical tests. Erythrocytes and hemoglobin can be used as parameters of physiological adaptation to cave habitats. This is indicated by an increase in erythrocytes and hemoglobin followed by a decrease in air temperature and oxygen levels, as well as increased humidity and ammonia levels in cave habitats. These study results can be supported if similar studies analyze the hematological profile of non-cave-roosting bat. It is recommended that future studies analyze the hematological profile of non-cave-roosting bats around the caves of the studies or in areas with similar conditions.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88979919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0714
R. Mumtaz, M. A. Khan, T. Mubeen, M. Azeem, M. Zubai̇r
Wounds have always been a serious hazard to the public health around the world. Currently available treatments are limited due to their side effects and high cost. Plants are being used as a good source of medicines due to their safety, and ease of access. The objective of the study was to examine the potential cell proliferation and migration activities of Syzygium aromaticum extracts to understand the wound healing property of this plant. Methanol-, ethanol-and acetone-based extracts were prepared from S. aromaticum flower buds and tested in a scratch assay using a human retinal pigment epithelial (RPE) cell line. Polyphenolic contents in the extracts of S. aromaticum responsible for wound healing potential were identified using high performance liquid chromatography (HPLC). The study revealed that the majority of examined extracts stimulated cell proliferation of RPE cells. The activities were highest when the extracts were prepared using acetone as solvent at 0.05 mg/mL concentration. Acetone-and ethanol-based extracts yielded the best results at 0.05 mg/mL whereas methanol-based extract produced the highest result at 0.5 mg/mL. Thus, the present study provides better understanding of the traditional use of S. aromaticum for the treatment of wounds. However, further detailed studies are needed to optimize the concentration of different compounds of S. aromaticum for wound healing treatment.
{"title":"SYZYGIUM AROMATICUM (L.) EXTRACTS STIMULATE THE CELL PROLIFERATION IN AN IN VITRO SCRATCH ASSAY","authors":"R. Mumtaz, M. A. Khan, T. Mubeen, M. Azeem, M. Zubai̇r","doi":"10.36899/japs.2023.5.0714","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0714","url":null,"abstract":"Wounds have always been a serious hazard to the public health around the world. Currently available treatments are limited due to their side effects and high cost. Plants are being used as a good source of medicines due to their safety, and ease of access. The objective of the study was to examine the potential cell proliferation and migration activities of Syzygium aromaticum extracts to understand the wound healing property of this plant. Methanol-, ethanol-and acetone-based extracts were prepared from S. aromaticum flower buds and tested in a scratch assay using a human retinal pigment epithelial (RPE) cell line. Polyphenolic contents in the extracts of S. aromaticum responsible for wound healing potential were identified using high performance liquid chromatography (HPLC). The study revealed that the majority of examined extracts stimulated cell proliferation of RPE cells. The activities were highest when the extracts were prepared using acetone as solvent at 0.05 mg/mL concentration. Acetone-and ethanol-based extracts yielded the best results at 0.05 mg/mL whereas methanol-based extract produced the highest result at 0.5 mg/mL. Thus, the present study provides better understanding of the traditional use of S. aromaticum for the treatment of wounds. However, further detailed studies are needed to optimize the concentration of different compounds of S. aromaticum for wound healing treatment.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90598588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-20DOI: 10.36899/japs.2023.5.0706
M. Munir, I. Fatima, B. Aftab, A. Sheikh, A. Shabir, J. Ahmad, M. S. Ahmed
Stevia rebaundiana is containing sweet phytochemicals and is a zero-caloric natural alternative to artificial sweeteners. The direct organogenesis of S. rebaundiana was carried out using apical bud explant in vitro . The Murashige and Skoog medium was used and the culture was set up under optimized conditions of 24±2°C temperature, and 16 hours of light exposure with cool, white fluorescent light. The seven different concentrations of benzylaminopurine (BAP) and IAA (Indole-3-acetic acid) (0.01, 0.02, 0.03, 0.04, 0.05, 0.06 and 0.07mg/L) were employed for shoot development and root development respectively. The results revealed that of all the tested concentrations of BAP used for shoot development, 0.07mg/L proved to be best for shoot initiation after 6 days of culture. On the other hand, the suitable concentration of IAA at which root initiation was observed after 7 days of subculture, was 0.06mg/L. The study can be beneficial for the researchers, food industry and growers for commercial production of the S.rebaudiana nursery.
{"title":"DEVELOPMENT OF TISSUE CULTURE PROTOCOL FOR MASS PRODUCTION OF STEVIAREBAUDIANA (BERTONI) BERTONI","authors":"M. Munir, I. Fatima, B. Aftab, A. Sheikh, A. Shabir, J. Ahmad, M. S. Ahmed","doi":"10.36899/japs.2023.5.0706","DOIUrl":"https://doi.org/10.36899/japs.2023.5.0706","url":null,"abstract":"Stevia rebaundiana is containing sweet phytochemicals and is a zero-caloric natural alternative to artificial sweeteners. The direct organogenesis of S. rebaundiana was carried out using apical bud explant in vitro . The Murashige and Skoog medium was used and the culture was set up under optimized conditions of 24±2°C temperature, and 16 hours of light exposure with cool, white fluorescent light. The seven different concentrations of benzylaminopurine (BAP) and IAA (Indole-3-acetic acid) (0.01, 0.02, 0.03, 0.04, 0.05, 0.06 and 0.07mg/L) were employed for shoot development and root development respectively. The results revealed that of all the tested concentrations of BAP used for shoot development, 0.07mg/L proved to be best for shoot initiation after 6 days of culture. On the other hand, the suitable concentration of IAA at which root initiation was observed after 7 days of subculture, was 0.06mg/L. The study can be beneficial for the researchers, food industry and growers for commercial production of the S.rebaudiana nursery.","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72960780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-14DOI: 10.36899/japs.2023.1.0615
{"title":"ROLE OF HERBS AS ANTHELMINTIC IN THE CONTROL OF PARASCARIASIS IN EQUINES","authors":"","doi":"10.36899/japs.2023.1.0615","DOIUrl":"https://doi.org/10.36899/japs.2023.1.0615","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78075163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-20DOI: 10.36899/japs.2023.1.0609
{"title":"MOLECULAR ANALYSIS OF HEXON AND FIBER GENES OF FOWL AVIADENOVIRUSES ISOLATED FROM FIELD CASES OF INCLUSION BODY HEPATITIS","authors":"","doi":"10.36899/japs.2023.1.0609","DOIUrl":"https://doi.org/10.36899/japs.2023.1.0609","url":null,"abstract":"","PeriodicalId":22588,"journal":{"name":"The Journal of Animal and Plant Sciences","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81127532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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