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Analysis of Varietal Differences in Pre-harvest Sprouting of Rice using RNA-Sequencing 利用rna测序分析水稻收获前发芽的品种差异
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.274
MyoungGoo Choi, Hyen-Seok Lee, Woon-Ha Hwang, Seo-Yeong Yang, Yun-Ho Lee, Chung-gun Lee, S. Yun, Jae-Hyeok Jeong
Seed dormancy is an adaptive trait in which seeds do not germinate under unfavorable environmental conditions. Low dormancy seeds are easily germinated under optimal environmental conditions, and these characteristics greatly reduce the yield and quality of crops. In the present study, we compared the pre-harvest sprouting (PHS) rate of two cultivars, Joun and Jopyeong, using the Winkler scale after heading day and temperature of the test. The PHS rate increased as the Winkler scale after heading day increased from 700°C to 1100°C and the temperature of the test increased. In all conditions, the PHS rate of Jopyeong was higher than that of Joun. RNA-sequencing was used to analyze the cause of the high PHS rate. We analyzed the biological metabolic processes related to the abscisic acid (ABA) metabolite pathway using the KEGG mapper with selected differentially expressed genes in PHS seeds. We found that the expression of ABA biosynthesis genes (OsNCEDs) was down-regulated and that ABA catabolic genes (OsCYP707As) was up-regulated in PHS seeds. However, the quantitative real-time PCR results showed that Joun had a higher expression of OsNCEDs than that of Jopyeong, but OsCYP707As did not yield a significant result. Joun displayed higher ABA content than that of Jopyeong not only during ripeness time but also during PHS treatment. Taken together, we provided evidence that the ABA content remaining in the seed is important to the PHS rate, which is determined by the expression level of the ABA biosynthesis gene OsNCEDs.
种子休眠是种子在不利环境条件下不发芽的一种适应性性状。低休眠种子在最佳的环境条件下容易发芽,这些特点大大降低了作物的产量和品质。本研究采用Winkler比较法,比较了抽穗日和试验温度后两个品种“琼”和“佐平”的采前发芽率。抽穗日后Winkler标度从700℃升高到1100℃,试验温度升高,小PHS率随之升高。在所有条件下,佐平的小灵通率都高于琼。采用rna测序分析小灵通率高的原因。我们利用KEGG图谱分析了小灵通种子中与脱落酸(ABA)代谢途径相关的生物代谢过程。结果表明,小灵通种子中ABA生物合成基因OsNCEDs表达下调,ABA分解代谢基因OsCYP707As表达上调。然而,实时荧光定量PCR结果显示,Joun的osnced表达量高于Jopyeong,而OsCYP707As的表达量不显著。无论是成熟期还是小灵通处理期间,“琼”的ABA含量均高于“佐平”。综上所述,我们提供的证据表明,种子中残留的ABA含量对小灵通率很重要,而小灵通率是由ABA生物合成基因osnced的表达水平决定的。
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引用次数: 0
Current Research Trends of Wheat Transformation and Biotechnology 小麦转化与生物技术研究进展
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.386
Jae-Ryeong Sim, Sewon Kim, Su-Bin Lee, Beom-Gi Kim, S. Lee, Jong-Yeol Lee
Wheat is one of the world's top three crops and is an important staple crop, accounting for 20% of the nutrient calories consumed by the world’s population. However, due to its complex heterogeneous hexaploid chromosomes and vast genome of approximately 16 Gb, compared to those of other crops, molecular biology and biotechnology studies on wheat are lacking. In recent years, wheat genome analysis has been performed using the latest next-generation sequencing technology so that useful genes can be easily obtained, and wheat biotechnology research is accelerating in various fields. In this review, wheat transformation, an indispensable technique for developing new functional biotech wheat by revealing the function of wheat genes, is described in detail. In addition, the latest research results for overcoming plant diseases, abiotic stresses, and wheat-related diseases that are difficult to solve by classical breeding through wheat transformation and biotechnology are described.
小麦是世界三大作物之一,是重要的主粮作物,占世界人口营养热量消耗的20%。然而,由于其复杂的异质六倍体染色体和庞大的约16gb基因组,与其他作物相比,小麦的分子生物学和生物技术研究缺乏。近年来,利用最新的新一代测序技术对小麦进行基因组分析,使有用基因的获取变得更加容易,小麦生物技术在各个领域的研究正在加速。本文详细介绍了通过揭示小麦基因功能来开发新型功能转基因小麦的一项重要技术——小麦转化。此外,还介绍了利用小麦转化和生物技术克服传统育种难以解决的植物病害、非生物胁迫和小麦相关病害的最新研究成果。
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引用次数: 0
Changes in the Hyperspectral Characteristics of Wheat Plants According to N Top-dressing Rates at Various Growth Stages 不同生育期氮素追肥量对小麦植株高光谱特性的影响
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.377
Jae Gyeong Jung, Y. Lee, J. Choi, G. Song, Jonghan Ko, Kyungdo Lee, S. Shim
Recently, wheat consumption has been increasing in Korea, requiring increased production. Nitrogen fertilization is a critical determinant in crop yield; therefore, it is necessary to optimize the nitrogen fertilization regime with current trends that emphasize the minimum impact of nitrogen fertilizer on the environment. In this study, both nondestructive spectral analysis using a hyperspectral camera and growth analysis were performed to determine the optimal N top-dressing rates after heading. The nitrogen application regimes consisted of three conditions according to the secondary top-dressing rate: N4:3:0 (0 kg 10 a ), N4:3:3 (2.73 kg 10 a-1), and N4:3:6 (5.46 kg 10 a -1). Subsequently, growth and physiological investigations were performed at the jointing, heading, and ripening stages of wheat, and spectral investigations were conducted. On April 29, as the nitrogen fertilization rate was increased to N4:3:3 and N4:3:6, plant height and grain yield increased by 4% and 8%, and 8% and 52%, respectively, compared to those under N4:3:0. Leaf area index and SPAD value also increased by 13% and 24%, and 32% and 43%, respectively. The R (red), G (green), and B (blue) of leaf color were lowered by 15, 11, and 4 in N4:3:3 and 44, 34, and 18 in N4:3:6, respectively, as compared to the control. Grain yield was the highest at high top-dressing (N4:3:6), however, there was no difference between no top-dressing (N4:3:0) and intermediat top-dressing (N4:3:3). The reflectance analyzed using a hyperspectral camera showed a difference in the near-infrared (NIR) region on March 19, and on April 29, there was a difference both in the visible light region greater than 550 nm and the NIR region. Vegetation indices differed according to fertilization regime, except for the greenness index (GI). The results of this study showed that not only growth and physiological analysis but also spectral indices can be used to optimize the nitrogen top-dressing rate.
最近,国内小麦消费量不断增加,因此需要增加产量。氮肥是作物产量的关键决定因素;因此,有必要根据当前的趋势优化氮肥施用制度,以强调氮肥对环境的影响最小。在本研究中,利用高光谱相机进行非破坏性光谱分析和生长分析,以确定抽穗后的最佳氮肥追肥率。根据二次追肥速率,施氮量为N4:3:0 (0 kg - 10 a)、N4:3:3 (2.73 kg - 10 a-1)和N4:3:6 (5.46 kg - 10 a-1)。随后,在小麦拔节、抽穗和成熟阶段进行生长和生理研究,并进行光谱研究。4月29日,当施氮量增加到N4:3:3和N4:3:6时,株高和籽粒产量分别比N4:3:0时提高了4%和8%,提高了8%和52%。叶面积指数和SPAD值分别提高了13%和24%,32%和43%。与对照相比,N4:3:3处理叶片颜色R(红色)、G(绿色)和B(蓝色)分别降低了15、11和4,N4:3:6处理叶片颜色R(红色)、G(绿色)和B(蓝色)分别降低了44、34和18。籽粒产量以高追肥(N4:3:6)最高,不追肥(N4:3:0)与中追肥(N4:3:3)无显著差异。利用高光谱相机分析的反射率显示,3月19日在近红外(NIR)区域存在差异,4月29日在大于550 nm的可见光区域和近红外区域都存在差异。除绿度指数(GI)外,不同施肥方式的植被指数存在差异。本研究结果表明,不仅可以通过生长和生理分析,还可以通过光谱指标来优化氮肥追肥速率。
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引用次数: 0
Changes in Growth Characteristics of Waxy Corn ‘Ilmichal’ due to Low Temperature during the Seedling Stage 苗期低温对糯玉米' Ilmichal '生长特性的影响
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.426
Seung-Ho Jeon, Seung-Ka Oh, Hang-Yong Kim, Chae-In Na, Hui-su Bae, Young-Son Cho
To determine the damages to waxy corn caused by low temperature weather, we investigated the relationship between the temperature and duration of low temperature treatment and the changes in growth characteristics during the recovery period after the treatments in different growth periods. Growth inhibition started in the low temperature group treated at temperatures below 5°C for three days. The inhibition ratio (IR) was more than 22% or more in all sample groups. As the treatment duration increased and the Temperature decreased, the growth was more greatly inhibited than that in the control. The IR was the highest at 27% or more in the 2nd leaf stage group treated at below 5°C for 5 days. The IR was in the order of 2nd leaf stage > 1st leaf stage > coleoptile. The IR during recovery was the highest in the 2nd leaf stage group treated at -3°C degree for 7 days, nd the values were 82% and 98% for NDVI and Fv/Fm, respectively. Especially, all groups treated at -3°C showed either no changes or decreases in the growth characteristics. As a result, growth inhibition increased as the temperature decreased, and as the duration of the low temperature increased. The degree of damage was in the order of 2nd leaf stage > 1st leaf stage > coleoptile. All early seedlings stopped growing and withered when exposed to temperatures at or below -3°C for 3 days or more.
为确定低温天气对糯玉米的危害程度,研究了低温处理温度与处理时间的关系,以及不同生育期处理后恢复期糯玉米生长特性的变化。低温组在低于5°C的温度下处理3天,开始出现生长抑制。各样品组的抑制率(IR)均大于22%。随着处理时间的延长和温度的降低,其生长受到的抑制比对照更大。在5℃以下处理5 d的第2叶期组IR最高,达27%以上。IR大小为叶片2期>叶片1期>胚芽组织。在-3℃处理7 d的第2叶期组,恢复期间的IR最高,NDVI和Fv/Fm分别达到82%和98%。特别是,在-3°C处理下,所有组的生长特性没有变化或下降。因此,随着温度的降低和低温持续时间的增加,生长抑制作用增强。受害程度为叶片2期>叶片1期>胚芽鞘。当暴露在-3℃或低于-3℃的温度下3天或更长时间时,所有早期幼苗都停止生长并枯萎。
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引用次数: 0
Effects of High Temperature and Drought on Yield and Quality of Soybean 高温干旱对大豆产量和品质的影响
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.346
P. Shin, W. Sang, Junhwan Kim, Y. Lee, Jae-kyeong Baek, Dongwon Kwon, Jung-Il Cho, andMyung-Chul Seo
Currently, many studies are being conducted to cope with climate changes due to global warming and abnormal weather. The objective of this study was to investigate the effects of weather on the growth, yield components, and quality of soybeans using weather data from 2017 and 2018. The average temperature in 2018 was higher than that in 2017 from R1 to R5 of the growth stage for all cultivars. On the other hand, precipitation in 2018 was reduced compared to that in 2017 for Daewon and Daepung-2ho. It was observed that the flowering date in 2018 was earlier than that in 2017 for Daewon and Daepung-2ho, but the flowering date for Pungsannamul in 2018 was similar to that in 2017. Simulating soil water content with the estimation model (AFKAE0.5) determined that there were fewer drought dates in 2017 than those in 2018, and drought lasted from R1 to early R5 of the growth stage in 2018. Soybean growth in 2017 was better than that in 2018, and seed yield and 100-seed weight of soybean were higher in 2017 than those in 2018 for all cultivars. The seed size in 2017 was larger than that in 2018 for all cultivars. Oil content in 2017 was higher than that in 2018; in particular, the difference between both years was observed for Daewon and Daepung-2ho. Protein content was higher in 2018 than that in 2017; however, there were different levels for each cultivar. Thus, these results indicate that the yield component and quality of soybeans are affected by high temperature and drought.
目前,由于全球变暖和异常天气,人们正在进行许多研究来应对气候变化。本研究的目的是利用2017年和2018年的天气数据,研究天气对大豆生长、产量组成部分和质量的影响。各品种生育期R1 ~ R5, 2018年平均气温均高于2017年。相反,大原和大平二湖地区2018年的降水量比2017年有所减少。据观察,大院和大平二湖2018年的花期比2017年早,但丰山南村2018年的花期与2017年相似。利用估算模型(AFKAE0.5)模拟土壤含水量,发现2017年干旱天数少于2018年,干旱持续时间为生长期R1 ~ R5前期。2017年大豆生长情况好于2018年,各品种大豆单产和百粒重均高于2018年。所有品种2017年的种子大小均大于2018年。2017年含油量高于2018年;特别是大院和大平二湖的年份差异较大。蛋白质含量2018年高于2017年;但不同品种间含量不同。因此,这些结果表明,高温和干旱影响大豆的产量组成和品质。
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引用次数: 1
Review on Adaptability of Rice Varieties and Cultivation Technology According to Climate Change in Korea 韩国水稻品种和栽培技术对气候变化的适应性研究进展
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.327
M. Seo, Joon-Hwan Kim, K. Choi, Y. Lee, W. Sang, Hyeon-Suk Cho, Jung-Il Cho, P. Shin, Jae-kyeong Baek
In recent years, the temperature of Korea has been rapidly increasing due to global warming. Over the past 40 years, the temperature of Korea has risen by about 1.26°C compared to that in the early 1980s. By region, the west region of the Gangwon Province was the highest at 1.76°C and the Jeonnam Province was the lowest at 0.96°C. As the temperature continues to rise, it is expected that the rice yield will decrease in the future using the current standard cultivation method. As a result of global warming, the periods in which rice cultivation could be possible in regions each year has increased compared those to the past, showing a wide variety from 110 days in Taebaek to 180 days in Busan and Gwangyang. In addition, the transplanting time was delayed by 3-5 days in all regions. The average annual yield of rice showed an increasing trend when we analyzed the average productivities of developed varieties for cooked rice since the 1980s, especially in the early 1990s, which showed a rapid increase in productivity. The relationship between the average temperature at the time of development and the rice yield was divided into the periods before and after 1996. The higher the average temperature, the lower the yield of the developed varieties until 1996. However, since 1996, the increase in the average temperature did not show a trend in the productivity of the developed varieties. The climate change adaptability of developed rice varieties was investigated by analyzing the results of growing crops nationwide from 1999 to 2016 and the change in the annual yields of developed varieties and recently developed varieties as basic data to investigate the growth status of the crops in the country. As a result of annual comparisons of the yields of Taebongbyeo (2000) and Ungwangbyeo (2004) developed in the early 2000s for Odaebyeo, which was developed in the 1980s, the annual yields were relatively higher in varieties in the 2000s despite the increase in temperature. The annual yields of Samgwangbyeo (2003) and Saenuribyeo (2007), which were recently developed as mid-late-type varieties, were higher than those of an earlier developed variety called Chucheongbyeo, which was developed in the 1970s. Despite the rapid increase in temperature, rice cultivation technology and variety development are well adapted to climate change. However, since the biological potential of rice could reach its limit, it is necessary to develop continuous response technology.
近年来,由于全球变暖,韩国的气温急剧上升。在过去的40年里,韩国的气温比20世纪80年代初上升了约1.26摄氏度。从地区来看,江原道西部地区最高,为1.76度,全南地区最低,为0.96度。随着气温的持续升高,按照目前的标准栽培方法,预计未来水稻产量将会下降。受全球变暖的影响,各地区每年可以种植水稻的时间比过去有所增加,从太白的110天到釜山和光阳的180天,变化很大。此外,各地区移栽时间均延迟3-5天。分析20世纪80年代以来,特别是90年代初,我国水稻年平均产量呈上升趋势,水稻年平均产量增长较快。发育时平均气温与水稻产量的关系分为1996年前后两个时期。1996年以前,平均温度越高,所开发品种的产量越低。但自1996年以来,平均气温的升高对发育品种的产量影响不明显。通过对1999 - 2016年全国作物生长结果的分析,以发达品种和新开发品种的年产量变化为基础数据,调查水稻品种的气候变化适应性,了解全国作物生长状况。以20世纪80年代栽培的五大田为对象,对21世纪初栽培的太丰碑(2000年)和云光碑(2004年)的产量进行了年度比较,结果发现,虽然气温有所上升,但各品种的产量在21世纪初相对较高。最近开发的中后期品种“三光别”(2003年)和“新国家别”(2007年)的年产量比70年代开发的早期品种“初清别”要高。尽管全球气温快速上升,但水稻栽培技术和品种发展对气候变化的适应能力较强。然而,由于水稻的生物潜力可能达到极限,因此有必要开发连续响应技术。
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引用次数: 3
Changes in Chemical Properties and Effect on Germination of Radish Seed from Aeration of Co-digestate Fertilizers 共消化肥料曝气对萝卜种子化学性质的变化及萌发的影响
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.508
Ji-Eun Byeon, Hong-Ju Lee, J. Ryoo, Sun-Goo Hwang
This study aimed to determine the minimum period of aeration treatment of co-digestate to develop it as liquid fertilizer and the chemical changes that occur in the aerobic liquefying process. The co-digestates were divided into three types depending on their additives: swine slurry anaerobic digestate (SS AD), swine slurry 70% + cow slurry 30% anaerobic digestate (SS + CS AD), and swine slurry 70% + apple pomace 30% anaerobic digestate (SS + AP AD). The pH of all co-digestates increased rapidly after 3 days of aerobic treatment, but had slightly decreased in SS AD after 9 days and in SS + CS AD and SS + AP AD after 15 days. All co-digestates showed a strongly reduced pH between 27 and 36 days of aeration treatment. SS AD had lower pH value, dissolved oxygen (DO), NH4-N, and NO3-N content under aerobic conditions than other co-digestates. To assess the fully decomposed liquid fertilizer, a germination test was performed on the undiluted and diluted co-digestate using the liquid fertilizer germination index (LFGI) method. The relative germination ratio, relative root elongation, and germination index of SS AD were higher than those of the others. When the LFGI method was used for the germination test, all co-digestates showed an appropriate germination index of 70 after 60 days of aeration treatment. Thus, we suggest that the minimum period of aeration treatment for co-digestates might be 60 days to develop the fully decomposed liquid fertilizer.
本研究旨在确定共消化液作为液体肥料进行曝气处理的最短时间,以及好氧液化过程中发生的化学变化。根据共消化液添加物的不同,将共消化液分为猪浆厌氧消化液(SS AD)、猪浆70% +牛浆30%厌氧消化液(SS + CS AD)和猪浆70% +苹果渣30%厌氧消化液(SS + AP AD) 3种类型。在好氧处理3天后,所有共消化液的pH均迅速升高,但在SS AD处理9天后,SS + CS AD和SS + AP AD处理15天后,pH略有下降。在曝气处理27 ~ 36天期间,所有共消化菌的pH值均显著降低。SS AD在好氧条件下具有较低的pH值、溶解氧(DO)、NH4-N和NO3-N含量。采用液肥发芽指数(LFGI)法对未稀释和稀释的共消化液进行了发芽试验,以评价液肥的完全分解效果。相对发芽率、相对根伸长和发芽指数均高于其他品种。采用LFGI法进行萌发试验时,所有共消化物在曝气处理60 d后的萌发指数均为70。综上所述,建议共消化菌曝气处理的最短时间为60 d,以开发充分分解的液肥。
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引用次数: 0
QTL Analysis to Improve and Diversify the Grain Shape of Rice Cultivars in Korea, Using the Long Grain japonica Cultivar, Langi 以长粒粳稻“琅吉”为例,对韩国水稻品种籽粒形状改良和多样化的QTL分析
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.303
Suk‐Man Kim, Hyun-Su Park, Chang-Min Lee, Man-Kee Baek, Young-chan Cho, J. Suh, O. Jeong
Rice grain shape is one of the key components of grain yield and market value. An understanding of the genetic basis of the variation in grain shape could be used to improve grain shape. In this study, we developed a total of 265 F2 individuals derived from a cross between japonica cultivars (Josaeng-jado and Langi) and used this population for quantitative trait locus (QLT) analysis. Correlation analysis was performed to identify relationships between grain traits (GL: grain length, GW: grain width, L/W: ratio of length to width, TGW: 1,000 grain weight). The grain shape was positively correlated with GL and TGW, and negatively correlated with GW. In QTL analysis associated with grain shape, one QTL for GL, qGL5, detected on chromosome 5, explained 20.3% of the phenotypic variation (PV), while two QTLs, qGW5 (PV=36.1) and qGW7 (PV=26.1), for GW were identified on chromosomes 5 and 7, respectively. Evaluation of the effects of each of the QTLs on the grain shape in the population showed a significant difference in the grain size in positive lines compared with the lines without the QTLs. According to the QTL combination of the allelic-types, the grain shape of the tested lines varied from semi-round type to long spindle-shaped type. The results of this study extend our knowledge about the genetic pool governing the diversity of grain shape in japonica cultivars and could be used to improve the grain shape of this species through marker-assisted selective breeding in Korea.
稻米粒形是决定粮食产量和市场价值的关键因素之一。了解籽粒形状变异的遗传基础可用于改善籽粒形状。本研究从一个粳稻品种(josaengjado和Langi)的杂交群体中获得265个F2个体,并利用该群体进行了数量性状位点(QLT)分析。对籽粒性状(GL:粒长,GW:粒宽,L/W:长宽比,TGW:千粒重)进行相关性分析。籽粒形状与GL和TGW呈正相关,与GW负相关。在与籽粒形状相关的QTL分析中,在第5染色体上检测到1个gl5 qGL5,解释了20.3%的表型变异(PV),而在第5染色体和第7染色体上分别检测到两个gl5 qGW5 (PV=36.1)和qGW7 qGW7 (PV=26.1)。评价各qtl对群体籽粒形状的影响表明,阳性株系的籽粒大小与未添加qtl的株系相比存在显著差异。根据等位基因型的QTL组合,供试品系的籽粒形状从半圆形到长纺锤形不等。本研究的结果扩展了我们对粳稻品种籽粒形状多样性的基因库的认识,并可用于通过标记辅助选择育种来改善该品种的籽粒形状。
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引用次数: 1
Effects of the Slow-releasing Fertilizer and Sowing Date on Waxy Corn “Mibaek 2” Propagated through Double Cropping without Tillage in the Middle Region of Korea 缓释肥和播期对韩国中部地区免耕两熟糯玉米米白2号的影响
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.477
Jae-Wung Lee, Se-Gu Hwang, Hyeran Moon, Ik-Jei Kim, Young Ho Kim
This study was conducted to investigate the effects of slow-releasing fertilizer and sowing date on waxy corn propagated through double cropping without tillage. “Mibaek 2” was sown for first cropping on March 25, April 5 , and April 15, and for second cropping on July 5, July 15, and July 25 in 2018-2019. In order to save labor, slow-releasing fertilizer was utilized only one time before sowing. The accumulated temperature from sowing to silking was about 590-700°C. It took 65-77 days when “Mibaek 2” was sown in early April, but the one sown in early July took 42-52 days. In the first cropping, the culm length and ear length caused by the sowing date had no statistical significance, but the kernel set length was the highest at 123 cm in the sowing district on April 5. The weight of marketable ears was the highest at 100%, in addition to soil testing-based recommended fertilization. Meanwhile, in the second cropping, culm length, ear length, and yield were less compared to the first cropping. The culm length, kernel set length, ear length, and seed set length decreased as sowing date was delayed. The number and yield of marketable ears were the highest at 100%, in addition to soil testing-based recommended fertilization like in the first cropping. It has been found that securing yield by July 15 to finish the second seeding of the crop in the central part of Korea is advantageous. These results of this study will be helpful to farmers for the double cropping of waxy corn cultivation and management.
本试验研究了缓释肥和播期对免耕两熟糯玉米的影响。“米白2号”在2018 ~ 2019年分别于3月25日、4月5日、4月15日、4月15日、7月5日、7月25日播种第一季和第二季。为了节省劳动力,缓释肥在播种前只施用一次。从播种到吐丝的积温约为590 ~ 700℃。4月初播种的“米白2号”需要65 ~ 77天,而7月初播种的“米白2号”需要42 ~ 52天。播期对第一季茎长和穗长影响无统计学意义,但4月5日播种区粒长最高,为123 cm。除土壤试验推荐施肥外,可售穗重量最高,达到100%。第二季的茎长、穗长和产量均低于第一季。随着播期的推迟,茎长、粒长、穗长和种子长均呈下降趋势。除首季土壤试验推荐施肥外,可售穗数和产量最高,达到100%。据调查,在7月15日之前确保产量,在中部地区完成第二次播种是有利的。本研究结果对糯玉米双季作的栽培管理具有一定的指导意义。
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引用次数: 0
Evaluation of Morphological Traits and Genetic Composition in Melon Germplasm 甜瓜种质形态性状及遗传组成的评价
Pub Date : 2020-12-01 DOI: 10.7740/KJCS.2020.65.4.485
Seung-bae Lee, I. Jang, D. Hyun, Jung Ro Lee, Seong-Hoon Kim, Eunae Yoo, Sookyeong Lee, G. Cho, A. J. Lee
Melon (Cucumis melo L.), one of the most important fruit crop species, is cultivated worldwide. In this study, a total of 206 melon accessions conserved at the National Agrobiodiversity Center (NAC) in RDA were characterized for nine morphological characteristics according to the NAC descriptor list. In addition, to confirm the genetic composition of each melon accession, genetic profiling was performed using 20 SSR markers. Among the 206 melon accessions, 159 (77.2%) were collected from Asia. The color of fruit flesh and skin were mostly ‘white’ (56.0%) and ‘green’ (49%), respectively. Days to female flowering (FD) and maturity (MD) of the accessions ranged from 58 to 72 and 17 to 63, respectively. The fruit length and width of the accessions ranged from 6.0 to 29.3 and 3.6 to 17.2 cm, respectively. The sugar content (SU) ranged from 2.5% to 13.2% with an average of 7.0%. In correlation analysis, SU showed positive and negative correlations with MD and FD, respectively. The accessions were classified into four clusters by cluster analysis. From the results of genetic profiling using 20 SSR markers, three accessions (K189118, K100486, and K190292) were expected to be inbred lines among 206 melon accessions. These results could expand the knowledge of the melon germplasm, providing valuable material for the development of new melon varieties to suit consumer tastes.
甜瓜(Cucumis melo L.)是世界上最重要的水果作物之一。以国家农业生物多样性中心(NAC)保存的206份甜瓜种质资源为研究对象,根据NAC描述符表对9个形态特征进行了分析。此外,利用20个SSR标记进行遗传图谱分析,以确定各甜瓜品种的遗传组成。206份甜瓜中,159份来自亚洲,占77.2%。果肉和果皮的颜色分别为“白色”(56.0%)和“绿色”(49%)。雌花期(FD)为58 ~ 72天,成熟期(MD)为17 ~ 63天。果实长为6.0 ~ 29.3 cm,宽为3.6 ~ 17.2 cm。含糖量(SU)为2.5% ~ 13.2%,平均为7.0%。在相关分析中,SU与MD和FD分别呈正相关和负相关。通过聚类分析将其分为4类。从20个SSR标记的遗传分析结果来看,在206份甜瓜材料中,3份材料(K189118、K100486和K190292)有望成为自交系。这些结果可以扩大对甜瓜种质资源的认识,为开发适合消费者口味的甜瓜新品种提供有价值的材料。
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引用次数: 2
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The Korean Journal of Crop Science
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