Pub Date : 2021-08-01DOI: 10.29261/pakvetj/2020.099
Dong Chen
The aim of the study was to evaluate the variations in the rumen ecosystem at genus level and predicted functions of rumen bacteria by using whole crop corn silage (WCS), whole crop rice silage (WRS) or rice straw (RS) as forage sources in beef cattle ration. Ruminal digesta samples from 10 bulls per treatment were collected at day 60 of experimental period. The PCoA plots based on the Bray-Curtis distance matrix (BDM) expressed separation between WRS and WCS , WRS and RS using PC1 (P<0.05, 45.56%). The PCoA plots based on BDM also expressed separation among WRS, WCS and RS group using PC2 (P<0.05, 11.95%). Microbiota composition resutls at genus level showed that the most abundant genera were Prevotella (13.37%) and Ruminococcus (4.00%). Comparison of treatments represented that Prevotella, Treponema and Anaerostipes were higher in bulls fed WCS forage. Clostridium, Anaeroplasma and RFN20 were higher in bulls fed RS forage than animals fed WCS and WRS. Butyrivibrio was higher in WRS and RS treatments than WCS treatment group. Pseudobutyrivibrio was higher in bulls fed WRS forage than animals fed RS and WCS forage. Fibrobacter was higher in RS and WCS as compared to WRS. The results of functional alteration of rumen microbiota in different experimental groups represented that the leading modified function of the microbiome was the transporter. Based on findings of current study, it is concluded that microbial community at genus level in the rumen of bulls was highly altered by forage type.
{"title":"Rumen Microbial Community and Functions of Rumen Bacteria under Different Feeding Regime","authors":"Dong Chen","doi":"10.29261/pakvetj/2020.099","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.099","url":null,"abstract":"The aim of the study was to evaluate the variations in the rumen ecosystem at genus level and predicted functions of rumen bacteria by using whole crop corn silage (WCS), whole crop rice silage (WRS) or rice straw (RS) as forage sources in beef cattle ration. Ruminal digesta samples from 10 bulls per treatment were collected at day 60 of experimental period. The PCoA plots based on the Bray-Curtis distance matrix (BDM) expressed separation between WRS and WCS , WRS and RS using PC1 (P<0.05, 45.56%). The PCoA plots based on BDM also expressed separation among WRS, WCS and RS group using PC2 (P<0.05, 11.95%). Microbiota composition resutls at genus level showed that the most abundant genera were Prevotella (13.37%) and Ruminococcus (4.00%). Comparison of treatments represented that Prevotella, Treponema and Anaerostipes were higher in bulls fed WCS forage. Clostridium, Anaeroplasma and RFN20 were higher in bulls fed RS forage than animals fed WCS and WRS. Butyrivibrio was higher in WRS and RS treatments than WCS treatment group. Pseudobutyrivibrio was higher in bulls fed WRS forage than animals fed RS and WCS forage. Fibrobacter was higher in RS and WCS as compared to WRS. The results of functional alteration of rumen microbiota in different experimental groups represented that the leading modified function of the microbiome was the transporter. Based on findings of current study, it is concluded that microbial community at genus level in the rumen of bulls was highly altered by forage type.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"101 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73808675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.079
S. Farooq
Mycoplasma gallisepticum (MG) is a pathogen of concern for poultry. Present study was conducted to determine the biological characteristics of a field isolate of MG, recovered from an MG-affected flock. This isolation was made through conventional method of MG cultivation, using modified Frey’s media after confirming the isolate by polymerase chain reaction (PCR). A total of 48 birds were segregated into experimental group (32 birds) and the control group (16 birds). To appraise primary site of infection, MG broth propagated culture containing 1x106 CFU/ml was inoculated intratracheally to each bird in the experimental group, whereas the control group was sham inoculated by uninoculated broth. The clinical signs and symptoms were recorded daily from day 1 to 21 post-infection (p.i.). Seroconversion monitoring was carried out, at day 5, 10, 15, 20 p.i. by Serum Plate Agglutination test (SPA) and Enzyme Linked Immunosorbent Assay (ELISA). To determine the dissemination pattern of MG, birds were sacrificed according to plan, swabbed from various organs and subjected to MG-specific PCR. Tracheal lesions and air sac lesions were scored after necropsy. Clinically, mild signs of respiratory discomfort were observed on day 5 p.i., which intensified on day 9 to 21 p.i. in the experimental group. PCR of tracheal swab samples was positive from day 7 to 21 p.i., and the swabs collected from lungs were positive for MG from day 9 to 21 p.i. The study concluded that, MG isolate from field showed limited dissemination pattern and is restricted to respiratory tract.
{"title":"Biological Characterization of Locally Circulating Mycoplasma gallisepticum in Poultry","authors":"S. Farooq","doi":"10.29261/PAKVETJ/2020.079","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.079","url":null,"abstract":"Mycoplasma gallisepticum (MG) is a pathogen of concern for poultry. Present study was conducted to determine the biological characteristics of a field isolate of MG, recovered from an MG-affected flock. This isolation was made through conventional method of MG cultivation, using modified Frey’s media after confirming the isolate by polymerase chain reaction (PCR). A total of 48 birds were segregated into experimental group (32 birds) and the control group (16 birds). To appraise primary site of infection, MG broth propagated culture containing 1x106 CFU/ml was inoculated intratracheally to each bird in the experimental group, whereas the control group was sham inoculated by uninoculated broth. The clinical signs and symptoms were recorded daily from day 1 to 21 post-infection (p.i.). Seroconversion monitoring was carried out, at day 5, 10, 15, 20 p.i. by Serum Plate Agglutination test (SPA) and Enzyme Linked Immunosorbent Assay (ELISA). To determine the dissemination pattern of MG, birds were sacrificed according to plan, swabbed from various organs and subjected to MG-specific PCR. Tracheal lesions and air sac lesions were scored after necropsy. Clinically, mild signs of respiratory discomfort were observed on day 5 p.i., which intensified on day 9 to 21 p.i. in the experimental group. PCR of tracheal swab samples was positive from day 7 to 21 p.i., and the swabs collected from lungs were positive for MG from day 9 to 21 p.i. The study concluded that, MG isolate from field showed limited dissemination pattern and is restricted to respiratory tract.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84201593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2021.006
K. Ali
Fifteen dogs were presented with complete blindness that progressed over 2-4 months. Diagnosis was confirmed that dogs had leishmaniasis through direct observation of the amastigotes within the blood cells, PCR testing and phylogenetic analysis. Gross pathologic and histopathologic examinations were performed for two dogs that were severely debilitated and humanely euthanized. Systemic involvement including decreased appetite (n=8), generalized weight loss (n=4), generalized lymphadenopathy (n=3), icterus (n=3), polyuria and polydepsia (n=2), lethargy (n=5) and four dogs were presented without any systemic involvement. All dogs had bilateral panophthalmia (n=30 eyes) manifested by cataract, anterior uveitis, posterior uveitis, retinal detachment, peri-ocular alopecia, conjunctivitis, blepharitis, keratoconjunctivitis and glaucoma. Detailed ultrasonographic ocular lesions were described; histopathological examination confirmed the ongoing changes within the eye. Leishmaniasis should be considered in the differential diagnosis of dogs with bilateral ocular involvement especially those not responding to symptomatic medicinal therapy.
{"title":"Bilateral Panophthalmia as a Late Sequel of Leishmaniasis in Dogs","authors":"K. Ali","doi":"10.29261/PAKVETJ/2021.006","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2021.006","url":null,"abstract":"Fifteen dogs were presented with complete blindness that progressed over 2-4 months. Diagnosis was confirmed that dogs had leishmaniasis through direct observation of the amastigotes within the blood cells, PCR testing and phylogenetic analysis. Gross pathologic and histopathologic examinations were performed for two dogs that were severely debilitated and humanely euthanized. Systemic involvement including decreased appetite (n=8), generalized weight loss (n=4), generalized lymphadenopathy (n=3), icterus (n=3), polyuria and polydepsia (n=2), lethargy (n=5) and four dogs were presented without any systemic involvement. All dogs had bilateral panophthalmia (n=30 eyes) manifested by cataract, anterior uveitis, posterior uveitis, retinal detachment, peri-ocular alopecia, conjunctivitis, blepharitis, keratoconjunctivitis and glaucoma. Detailed ultrasonographic ocular lesions were described; histopathological examination confirmed the ongoing changes within the eye. Leishmaniasis should be considered in the differential diagnosis of dogs with bilateral ocular involvement especially those not responding to symptomatic medicinal therapy.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78151083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.095
A. Guerrini
Few data are available about the prevalence of Salmonella enterica serovar Enteritidis (S.E.) and Salmonella enterica serovar Typhimurium (S.T.) in ornamental poultry in Italy. The aim of this study was to investigate the seroprevalence for S.E. and S.T. using serological tests and the prevalence of Salmonella spp. in eggs by culture methods. For this purpose, 240 serum samples and 216 eggs were sampled from asymptomatic and unvaccinated ornamental hens reared in 24 farms, located in 8 different Italian regions. As screening test, a Tube Serum Agglutination test (TSA) was performed on 231 out of 240 serum samples. Four out of 24 farms (16.67%) were serologically positive for Salmonella spp. for a total of 10 samples. These positive samples were confirmed using an ELISA test and the results show that 5/231 (2.16%) and 7/231 (3.03%) serum samples were positive for S.E. and S.T. respectively, and 2/231 (0.87%) for both serotypes. Among all farms, 2/24 (8.33%) were positive for S.E. and 4/24 (16.67%) for S.T. The analysis of eggs using culture methods gave negative results for both yolk and shell pools (0/48, 0.0%). The seroconversion associated with exposure to S.E./S.T. in ornamental poultry, poses a potential public health problem. This study confirms that S.E. and S.T. are widespread in studied backyard poultry farms as asymptomatic form, and animals as potential reservoirs of Salmonella. It is necessary to inform farmers that a regular and periodic control of animals, eggs or meat, is very important to prevention of Salmonella foodborne infections and their spread.
{"title":"Seroprevalence and Microbiological Monitoring in Eggs for Salmonella enterica Serovar Enteritidis and Salmonella enterica Serovar Typhimurium in Ornamental Chicken Flocks in Italy","authors":"A. Guerrini","doi":"10.29261/PAKVETJ/2020.095","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.095","url":null,"abstract":"Few data are available about the prevalence of Salmonella enterica serovar Enteritidis (S.E.) and Salmonella enterica serovar Typhimurium (S.T.) in ornamental poultry in Italy. The aim of this study was to investigate the seroprevalence for S.E. and S.T. using serological tests and the prevalence of Salmonella spp. in eggs by culture methods. For this purpose, 240 serum samples and 216 eggs were sampled from asymptomatic and unvaccinated ornamental hens reared in 24 farms, located in 8 different Italian regions. As screening test, a Tube Serum Agglutination test (TSA) was performed on 231 out of 240 serum samples. Four out of 24 farms (16.67%) were serologically positive for Salmonella spp. for a total of 10 samples. These positive samples were confirmed using an ELISA test and the results show that 5/231 (2.16%) and 7/231 (3.03%) serum samples were positive for S.E. and S.T. respectively, and 2/231 (0.87%) for both serotypes. Among all farms, 2/24 (8.33%) were positive for S.E. and 4/24 (16.67%) for S.T. The analysis of eggs using culture methods gave negative results for both yolk and shell pools (0/48, 0.0%). The seroconversion associated with exposure to S.E./S.T. in ornamental poultry, poses a potential public health problem. This study confirms that S.E. and S.T. are widespread in studied backyard poultry farms as asymptomatic form, and animals as potential reservoirs of Salmonella. It is necessary to inform farmers that a regular and periodic control of animals, eggs or meat, is very important to prevention of Salmonella foodborne infections and their spread.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90000586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.078
W. El-Deeb
The aim of this study was to correlate the serum levels of acute phase proteins (APPs) and cytokines in response to treatment by various antimicrobial agents in feedlots calves (FL) naturally infected with Mannheimia haemolytica (M. haemolytica) and Histophilus somni (H. somni). 840 feedlot calves in one farm in Al-Kharg region, Saudi Arabia were clinically examined for the presence of respiratory disease manifestations. The infection was confirmed using nasopharyngeal swabs. Blood samples from diseased animals were collected before and after (7 days) treatment for biochemical analysis of serum amyloid A (SAA), haptoglobin (HP) and cytokines tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), interleukin-1 β (IL1β), and interleukin-8 (IL-8). FL that were positive for M. haemolytica and/or H. somni (n=69) received treatment with one antibiotic, including tulathromycin (Tula; n=26 cases), florfenicol (FFC; n=19), tilmicosin (Tm; n=13), or ceftiofur (CEF; n=11) and one non-steroidal anti-inflammatory drug (Flunixin meglumine (FM; n=43) or phenylbutazone (PBZ; n= 26). We demonstrated the selective potent inhibitory effect of the administrated anti-inflammatory agents either FM or PBZ on the production of APPs and pro-inflammatory cytokines in FL infected with bovine respiratory disease (BRD). Our findings showed the antibacterial efficacy of FFC and Tm for the treatment of infected FL when administrated with either FM or PBZ. However, Tula was preferable to administrate in combination with FM for the treatment of FL with respiratory manifestations. Importantly, monitoring the sera level of Hp, IL-1β, and interleukin-8 (IL-8) in feedlots treated with either FM combined with Tula, FFC, or Tm or PBZ combined with FFC, and Tm has been effective in predicting the disease prognosis
{"title":"Clinical Efficacy of Antimicrobial Agents in Combination with Flunixin Meglumine and Phenylbutazone on Acute Phase Response in Respiratory Disease of Calves","authors":"W. El-Deeb","doi":"10.29261/PAKVETJ/2020.078","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.078","url":null,"abstract":"The aim of this study was to correlate the serum levels of acute phase proteins (APPs) and cytokines in response to treatment by various antimicrobial agents in feedlots calves (FL) naturally infected with Mannheimia haemolytica (M. haemolytica) and Histophilus somni (H. somni). 840 feedlot calves in one farm in Al-Kharg region, Saudi Arabia were clinically examined for the presence of respiratory disease manifestations. The infection was confirmed using nasopharyngeal swabs. Blood samples from diseased animals were collected before and after (7 days) treatment for biochemical analysis of serum amyloid A (SAA), haptoglobin (HP) and cytokines tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), interleukin-1 β (IL1β), and interleukin-8 (IL-8). FL that were positive for M. haemolytica and/or H. somni (n=69) received treatment with one antibiotic, including tulathromycin (Tula; n=26 cases), florfenicol (FFC; n=19), tilmicosin (Tm; n=13), or ceftiofur (CEF; n=11) and one non-steroidal anti-inflammatory drug (Flunixin meglumine (FM; n=43) or phenylbutazone (PBZ; n= 26). We demonstrated the selective potent inhibitory effect of the administrated anti-inflammatory agents either FM or PBZ on the production of APPs and pro-inflammatory cytokines in FL infected with bovine respiratory disease (BRD). Our findings showed the antibacterial efficacy of FFC and Tm for the treatment of infected FL when administrated with either FM or PBZ. However, Tula was preferable to administrate in combination with FM for the treatment of FL with respiratory manifestations. Importantly, monitoring the sera level of Hp, IL-1β, and interleukin-8 (IL-8) in feedlots treated with either FM combined with Tula, FFC, or Tm or PBZ combined with FFC, and Tm has been effective in predicting the disease prognosis","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90624947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.087
Muhammad Umar Zafar Khan
Clostridium perfringens (C. perfringens) is widely distributed in broiler chickens causing clinical and subclinical enteritis and is especially known for causing necrotic enteritis (NE). There are numerous reports of NE outbreaks in Pakistan as well as China but there is a lack of information related to PFGE profile from both the countries. To close this gap, we designed this study and obtained samples from broiler chicken farms located in 3 different regions of Pakistan and 4 different regions of China. A total of 79 fecal swabs (Pakistan=29; China=50) were collected and grown on FTA media. Further, isolates were grown on TSE agar and black colonies were selected for DNA extraction. All 79 isolates were tested for toxin profiles by PCR (α-gene; beta-2; netB gene) and PFGE profiling (pulsotypes analysis). Toxinotyping results revealed that all the isolates (n=50) from China were type A (α-toxin positive) while 23 and 6 isolates (n=29) from Pakistan were type A (α-toxin positive) and type G (α-toxin, NetB positive), respectively. Toxinotyping revealed α-toxin is highly prevalent in both the countries while from Pakistani isolates, NetB toxin was also detected. PFGE discriminated 79 isolates into 45 different PFGE patterns (pulsotypes). The analysis further showed different pulsotypes originating from China and Pakistan and isolates were subtyped by SmaI. The results showed high genetic polymorphism in C. perfringens even within the same strain. These preliminary findings of genetic variations will further help to design control strategies
{"title":"Genetic Diversity of Clostridium perfringens Strains Isolated from Broiler Chickens Revealed by PFGE Analysis in China and Pakistan","authors":"Muhammad Umar Zafar Khan","doi":"10.29261/PAKVETJ/2020.087","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.087","url":null,"abstract":"Clostridium perfringens (C. perfringens) is widely distributed in broiler chickens causing clinical and subclinical enteritis and is especially known for causing necrotic enteritis (NE). There are numerous reports of NE outbreaks in Pakistan as well as China but there is a lack of information related to PFGE profile from both the countries. To close this gap, we designed this study and obtained samples from broiler chicken farms located in 3 different regions of Pakistan and 4 different regions of China. A total of 79 fecal swabs (Pakistan=29; China=50) were collected and grown on FTA media. Further, isolates were grown on TSE agar and black colonies were selected for DNA extraction. All 79 isolates were tested for toxin profiles by PCR (α-gene; beta-2; netB gene) and PFGE profiling (pulsotypes analysis). Toxinotyping results revealed that all the isolates (n=50) from China were type A (α-toxin positive) while 23 and 6 isolates (n=29) from Pakistan were type A (α-toxin positive) and type G (α-toxin, NetB positive), respectively. Toxinotyping revealed α-toxin is highly prevalent in both the countries while from Pakistani isolates, NetB toxin was also detected. PFGE discriminated 79 isolates into 45 different PFGE patterns (pulsotypes). The analysis further showed different pulsotypes originating from China and Pakistan and isolates were subtyped by SmaI. The results showed high genetic polymorphism in C. perfringens even within the same strain. These preliminary findings of genetic variations will further help to design control strategies","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91272342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.090
Zulfia Hussain
Liver diseases are among the major health problems in Pakistan. The present study investigated the mechanism of hepatoprotection by cinnamon, cinnamaldehyde and kaempferol in Acetaminophen (APAP)-induced liver injury. Qualitative phytochemical analysis was performed for standardization of cinnamon ethanolic extract. For in-vivo evaluation, Balb/c mice were administered with cinnamon extract (200 mg/kg i.g.), cinnamaldehyde (10 mg/kg i.g.) and kaempferol (10 mg/kg i.g.) for 14 days followed by administration of APAP (200 mg/kg i.p.). At the end of trial, mice were dissected, and blood, liver and spleen samples were collected for biochemical, histopathological and apoptotic genes expression analysis. Statistical analysis was performed for significance of results. The results showed that the hepatic damage due to APAP administration for 8 hours in mice was apparent with increased severity. Cinnamon extract, cinnamaldehyde and kaempferol pretreatment suggested ameliorative effects on organ injury induced by APAP by decreasing the elevated serum levels of total proteins and bilirubin. In addition, APAP exerted severe alterations on liver histology without affecting spleen histology alongwith upregulation of Bad, Bax and Caspase-3 and downregulation of Bcl-2. However, cinnamon, cinnamaldehyde and kaempferol pretreatment ameliorated APAPinduced liver alterations and apoptosis, possibly through their antioxidant activity. In addition, cinnamaldehyde and kaempferol possessed comparable protective potential at 20-times less dose as compared to cinnamon extract alone, suggesting therapeutic potential at lower dose in APAP-induced liver injury and apoptosis.
{"title":"Protective Effects of Cinnamon, Cinnamaldehyde and Kaempferol against Acetaminophen-Induced Acute Liver Injury and Apoptosis in Mouse Model","authors":"Zulfia Hussain","doi":"10.29261/PAKVETJ/2020.090","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.090","url":null,"abstract":"Liver diseases are among the major health problems in Pakistan. The present study investigated the mechanism of hepatoprotection by cinnamon, cinnamaldehyde and kaempferol in Acetaminophen (APAP)-induced liver injury. Qualitative phytochemical analysis was performed for standardization of cinnamon ethanolic extract. For in-vivo evaluation, Balb/c mice were administered with cinnamon extract (200 mg/kg i.g.), cinnamaldehyde (10 mg/kg i.g.) and kaempferol (10 mg/kg i.g.) for 14 days followed by administration of APAP (200 mg/kg i.p.). At the end of trial, mice were dissected, and blood, liver and spleen samples were collected for biochemical, histopathological and apoptotic genes expression analysis. Statistical analysis was performed for significance of results. The results showed that the hepatic damage due to APAP administration for 8 hours in mice was apparent with increased severity. Cinnamon extract, cinnamaldehyde and kaempferol pretreatment suggested ameliorative effects on organ injury induced by APAP by decreasing the elevated serum levels of total proteins and bilirubin. In addition, APAP exerted severe alterations on liver histology without affecting spleen histology alongwith upregulation of Bad, Bax and Caspase-3 and downregulation of Bcl-2. However, cinnamon, cinnamaldehyde and kaempferol pretreatment ameliorated APAPinduced liver alterations and apoptosis, possibly through their antioxidant activity. In addition, cinnamaldehyde and kaempferol possessed comparable protective potential at 20-times less dose as compared to cinnamon extract alone, suggesting therapeutic potential at lower dose in APAP-induced liver injury and apoptosis.","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81513764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.082
A. Sallam
Cisplatin (CP) is a highly efficient remedy in cancer treatment, but it adversely affects the testicular tissue. This work assessed the ameliorative efficacy of Lcarnitine (LC) against CP induced oxidative stress in rat testis, via investigating testosterone level and tissue oxidative/antioxidant parameters, histological alterations, and immunohistochemical expressions of intermediate filaments (IFs) proteins; vimentin (VIM) and cytokeratin 18 (CK18). Twenty-eight rats were assigned into four groups (7 rats each) as follows; groups I and II received saline and LC (100 mg/kg b.wt.) respectively orally once daily for 30 days; group III were injected with a single dose of CP (7.5 mg/kg, IP), 27 days after starting the experiment. Group IV received both LC and CP. Injection of CP significantly decreased serum testosterone and glutathione reductase and catalase in the testicular tissues and elevated malondialdehyde. Histologically, testes of the CP treated group revealed marked degenerative changes. Also, overexpression of both VIM and CK18 in testicular tissues were recorded. However, the administration of LC with CP restored the biochemical parameters, histological and immunohistochemical pictures towards the normalcy. Accordingly, LC is recommended as a supplement with chemotherapy to ameliorate its oxidative stress. This is the first study investigating the immunohistochemical expressions of IFs proteins, VIM and CK18, following administration of LC as a protective agent against CP induced testicular toxicity in rats
{"title":"The Ameliorative Effects of L-Carnitine against Cisplatin-Induced Gonadal Toxicity in Rats","authors":"A. Sallam","doi":"10.29261/PAKVETJ/2020.082","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.082","url":null,"abstract":"Cisplatin (CP) is a highly efficient remedy in cancer treatment, but it adversely affects the testicular tissue. This work assessed the ameliorative efficacy of Lcarnitine (LC) against CP induced oxidative stress in rat testis, via investigating testosterone level and tissue oxidative/antioxidant parameters, histological alterations, and immunohistochemical expressions of intermediate filaments (IFs) proteins; vimentin (VIM) and cytokeratin 18 (CK18). Twenty-eight rats were assigned into four groups (7 rats each) as follows; groups I and II received saline and LC (100 mg/kg b.wt.) respectively orally once daily for 30 days; group III were injected with a single dose of CP (7.5 mg/kg, IP), 27 days after starting the experiment. Group IV received both LC and CP. Injection of CP significantly decreased serum testosterone and glutathione reductase and catalase in the testicular tissues and elevated malondialdehyde. Histologically, testes of the CP treated group revealed marked degenerative changes. Also, overexpression of both VIM and CK18 in testicular tissues were recorded. However, the administration of LC with CP restored the biochemical parameters, histological and immunohistochemical pictures towards the normalcy. Accordingly, LC is recommended as a supplement with chemotherapy to ameliorate its oxidative stress. This is the first study investigating the immunohistochemical expressions of IFs proteins, VIM and CK18, following administration of LC as a protective agent against CP induced testicular toxicity in rats","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75748979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.096
Basang Wang-Dui
A total of three family farms including Village No. 9 in Nima township, Nerong Naqu County (NQA); Village No. 11 in Nima township, Nerong Naqu County (NQB); and Yare township, Gegi County, Ali District (GJ) from three ecology yak populations (EYP) were selected for this study to identify the most optimized mode of exogenous male adult yak introduction (EMI) within EYP for solving the inbreeding problem caused by the small-scale yak husbandry system. Exogenous adult male yaks from the same EYP with different proportions (100% to NQA, 50% to NQB, and 0% to GJ) were introduced, and 10 microsatellites were used to detect the genetic diversity of these populations before (in 2017) and after (in 2019) the introduction of exogenous adult male yaks (EMI). Results showed that the divergence between the observed and the expected heterozygosity of the NQA and NQB populations was reduced in 2019, while the number of markers significantly deviating from Hardy–Weinberg equilibrium (P<0.05) and FIS (inbreeding coefficient) within populations decreased compared with that in 2017. In contrast, the FIS of GJ population without EMI continued to increase (from 0.011 to 0.033) over the years 2017 to 2019. Moreover, genetic differences between the populations (Pairwise Fixation index, FST) showed that EMI increased the genetic divergence between populations. Overall, this study shows that the introduction of exogenous male adult yaks not only effectively reduces the degree of population deviation from equilibrium but also decreases the inbreeding level within the population within a few generations. This study also provides a valuable management model for stable yak production on small sized family farms
{"title":"Exploration of the Exogenous Male Yak Introduction Breeding Model and its Effects on Tibetan Small-Sized Family Farms","authors":"Basang Wang-Dui","doi":"10.29261/PAKVETJ/2020.096","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.096","url":null,"abstract":"A total of three family farms including Village No. 9 in Nima township, Nerong Naqu County (NQA); Village No. 11 in Nima township, Nerong Naqu County (NQB); and Yare township, Gegi County, Ali District (GJ) from three ecology yak populations (EYP) were selected for this study to identify the most optimized mode of exogenous male adult yak introduction (EMI) within EYP for solving the inbreeding problem caused by the small-scale yak husbandry system. Exogenous adult male yaks from the same EYP with different proportions (100% to NQA, 50% to NQB, and 0% to GJ) were introduced, and 10 microsatellites were used to detect the genetic diversity of these populations before (in 2017) and after (in 2019) the introduction of exogenous adult male yaks (EMI). Results showed that the divergence between the observed and the expected heterozygosity of the NQA and NQB populations was reduced in 2019, while the number of markers significantly deviating from Hardy–Weinberg equilibrium (P<0.05) and FIS (inbreeding coefficient) within populations decreased compared with that in 2017. In contrast, the FIS of GJ population without EMI continued to increase (from 0.011 to 0.033) over the years 2017 to 2019. Moreover, genetic differences between the populations (Pairwise Fixation index, FST) showed that EMI increased the genetic divergence between populations. Overall, this study shows that the introduction of exogenous male adult yaks not only effectively reduces the degree of population deviation from equilibrium but also decreases the inbreeding level within the population within a few generations. This study also provides a valuable management model for stable yak production on small sized family farms","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73829848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-01DOI: 10.29261/PAKVETJ/2020.074
Lin-jing Feng
High somatic cell counts (SCCs) in milk significantly influence the quality of milk and give rise to substantial economic loss. The present study was aimed to investigate the effect of extreme heat and cold compared to other season and melatonin (MLT) on milk SCCs in Chinese crossbred (Nili-Ravi×Murrah) buffaloes. We collected the 1948 milk SCCs data records from 2012 to 2017 to explore the effect of different month in China on milk SCCs. Meanwhile, twenty buffaloes with relatively high milk SCCs were employed and randomly divided into two groups (T1 and T2, n=10 each group) to evaluate the effect of MLT treatment on milk SCCs, blood antioxidant activities and immune levels of buffaloes during summer in China. Results showed that the milk SCCs in high temperature seasons (July and August) and low temperature seasons (December, January and February) were significantly higher compared with other months (P<0.05). In summer, MLT treatment significantly reduced milk SCCs and increased the IgM and superoxide dismutase (SOD) levels in plasma on day 1 after MLT treatment, and then both IgM and SOD levels were decreased significantly. In conclusion, our study demonstrated that environmental temperature stress (heat and cold) caused the higher milk SCCs and MLT treatment improved the quality of milk by reducing SCCs suggesting that MLT could improve immune activity in buffaloes
{"title":"The Effect of Exogenous Melatonin on Milk Somatic Cell Count in Buffalo","authors":"Lin-jing Feng","doi":"10.29261/PAKVETJ/2020.074","DOIUrl":"https://doi.org/10.29261/PAKVETJ/2020.074","url":null,"abstract":"High somatic cell counts (SCCs) in milk significantly influence the quality of milk and give rise to substantial economic loss. The present study was aimed to investigate the effect of extreme heat and cold compared to other season and melatonin (MLT) on milk SCCs in Chinese crossbred (Nili-Ravi×Murrah) buffaloes. We collected the 1948 milk SCCs data records from 2012 to 2017 to explore the effect of different month in China on milk SCCs. Meanwhile, twenty buffaloes with relatively high milk SCCs were employed and randomly divided into two groups (T1 and T2, n=10 each group) to evaluate the effect of MLT treatment on milk SCCs, blood antioxidant activities and immune levels of buffaloes during summer in China. Results showed that the milk SCCs in high temperature seasons (July and August) and low temperature seasons (December, January and February) were significantly higher compared with other months (P<0.05). In summer, MLT treatment significantly reduced milk SCCs and increased the IgM and superoxide dismutase (SOD) levels in plasma on day 1 after MLT treatment, and then both IgM and SOD levels were decreased significantly. In conclusion, our study demonstrated that environmental temperature stress (heat and cold) caused the higher milk SCCs and MLT treatment improved the quality of milk by reducing SCCs suggesting that MLT could improve immune activity in buffaloes","PeriodicalId":22797,"journal":{"name":"The Pakistan Veterinary Journal","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85454373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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