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Application of Filtration to Recover Solubilized Proteins During pH-Shift Processing of Blue Whiting (Micromesistius poutassou); Effects on Protein Yield and Qualities of Protein Isolates 过滤技术在蓝白鱼ph移位加工中溶解蛋白回收中的应用对分离蛋白产量和品质的影响
Pub Date : 2011-01-18 DOI: 10.2174/1874256401105010001
Helgi Nolsøe, Sofia Marmon, I. Undeland
Previous studies of the pH-shift protein isolation process have shown that substantial amounts of solubilized proteins can be trapped in the sediments formed in the first centrifugation step of this process. As a strategy to improve the protein yield during pH-shift processing, the aim of this study was to evaluate how filtration as an alternative to centrifugation in the first separation step of pH-shift processing of blue whiting affected proteins yield and protein isolate characteristics (basic composition, polypeptide profiles, surimi gel quality and color attributes). The study comprised both the acid and alkaline versions of the method, and also fresh as well as frozen fish raw material. Results showed that the replacement of centrifugation with filtration substantially improved the protein yield by from about 38% to 62%, but also reduced the removal of lipid. There were no significant effects on gel quality. Protein isolates from fresh raw material were about 5 % whiter and frozen raw materials about 3% whiter with centrifugation as compared to filtration in the pHshift process. For surimi from fresh raw material centrifugation gave about 2 % whiter gels, while the gels from frozen raw material were about 3% whiter for filtered compared to centrifuged material. The whitest isolates and gels were obtained with acid processing of fresh blue whiting. Slight proteolytic breakdown resulting in fragments of 83 and 152 kDa was however noted with the acid process, especially when centrifugation was used.
先前对ph位移蛋白分离过程的研究表明,在该过程的第一个离心步骤中形成的沉积物中可以捕获大量的溶解蛋白。作为提高ph移位处理过程中蛋白质产量的策略,本研究的目的是评估在蓝白ph移位处理的第一步中,过滤替代离心对蛋白质产量和分离蛋白特性(基本组成、多肽谱、鱼米凝胶质量和颜色属性)的影响。这项研究包括酸法和碱法两种方法,以及新鲜和冷冻鱼原料。结果表明,用过滤代替离心后,蛋白质得率从38%提高到62%,但也降低了脂质去除率。对凝胶质量无显著影响。与在pHshift过程中过滤相比,从新鲜原料分离的蛋白在离心过程中白约5%,从冷冻原料分离的蛋白在离心过程中白约3%。对于新鲜原料的鱼糜,离心后的凝胶白度约为2%,而冷冻原料过滤后的凝胶白度约为3%。对鲜蓝白进行酸处理,得到了最白的分离物和凝胶。然而,在酸处理过程中,特别是在使用离心时,注意到轻微的蛋白质水解分解导致83和152 kDa的片段。
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引用次数: 17
Effects of Low-Methoxyl Pectin on Physicochemical and Sensory Properties of Reduced- Calorie Sorrel/ Roselle (Hibiscus sabdariffa L.) Jams 低甲氧基果胶对低热量酢浆草/芙蓉理化及感官特性的影响堵塞
Pub Date : 2010-12-21 DOI: 10.2174/1874256401004010048
J. Broomes, N. Badrie
Low-fat/ reduced calorie products were originally targeted for diabetics but now the focus is on healthiness. The global prevalence of diabetes is on the increase and may be addressed by controlling the total dietary carbohydrate and calorie intake. This study investigated the effects of low-methoxyl (LM) pectin on physiochemical and sensory properties of a reduced-calorie sorrel (Hibiscus sabdariffa) jam with sucralose. Sorrel calyces were treated with 0.5% pec- tolase to form puree. The puree was processed to jam at 90oC for 30 minutes, then at 100oC for 2 minutes upon the addi- tion of 8% sucralose and three levels (1.5, 2.0, 2.5%) of calcium added LM pectin for gelation. The addition of xanthan gum was at 2% w/w. The effects of higher LM pectin increased (P<0.01) the moisture content and texture but reduced wa- ter activity and total titratable acidity. The additional varying levels of LM pectin resulted (P<0.05) in lighter, more chro- matic and less bluish-red colored jam. The texture became firmer (P<0.01) with higher levels of LM pectin. A sorrel jam with 1.5% LM pectin had total soluble solids of 16oBrix, 0.96% citric acid and pH 3.3. This treatment was liked slightly to moderately in texture and overall acceptance. On storage the jam became less (P<0.01) bright and bluish-red on storage at 4o C for 28 days. Most panelists would purchase the reduced - calorie sorrel jam.
低脂/低热量产品最初是针对糖尿病患者的,但现在的重点是健康。全球糖尿病患病率呈上升趋势,可通过控制饮食中碳水化合物和卡路里的总摄入量来解决。本研究考察了低甲氧基(LM)果胶对含三氯蔗糖的低热量酢浆草(芙蓉)果酱的理化和感官特性的影响。用0.5%的乙醇酶处理酢浆草萼形成果浆。将果泥在90℃下加工成果酱30分钟,然后在100℃下加工2分钟,加入8%的三氯蔗糖和3个水平(1.5、2.0、2.5%)的钙,加入LM果胶凝胶化。黄原胶的添加量为2% w/w。高LM果胶的作用增加了水分含量和质构(P<0.01),但降低了水活性和总可滴定酸度。添加不同水平的LM果胶可使果酱颜色更浅、色度更高、蓝红色更少(P<0.05)。随着LM果胶添加量的增加,果胶的质地变得更加坚硬(P<0.01)。添加1.5% LM果胶的酸橙果酱的总可溶性固形物为16oBrix,柠檬酸含量为0.96%,pH为3.3。这种治疗方法在质地和总体接受度上略有到中等程度。40℃贮藏28 d时,果酱颜色变浅(P<0.01),呈蓝红色。大多数小组成员会购买低热量的酸橙果酱。
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引用次数: 26
Isolation of components with antimicrobial property from the donkey milk: a preliminary study. 驴奶中抗菌成分的分离初步研究。
Pub Date : 2010-09-03 DOI: 10.2174/1874256401004010043
F. Nazzaro, P. Orlando, F. Fratianni, R. Coppola
The presence of components in donkey milk, different from lysozyme, capable of inhibiting the growth of pathogenic microorganisms was investigated. Milk was acidified, hydrolyzed with pepsin, and analyzed by HPLC. The chromatographic fractions were collected in 5 main pools that, after the removal of lysozyme, were analyzed by micro- electrophoresis; antimicrobial activity was evaluated against the pathogens Bacillus cereus, Staphylococcus aureus, En- terococcus faecalis, and Escherichia coli. Hydrolyzed milk showed a protein pattern ranging from 0.78 to 25.2 kDa. It was active against all pathogenic strains tested, with inhibition halos ranging from 4.3 mm (against E.coli) to 17.4 mm (versus E.faecalis). Among the pools collected after HPLC step, pool "b" resulted to be the most rich in protein bands, most of them with a molecular weght less than 20.4 kDa, and the most effective against all the pathogens, except against E. coli. Pool "a", was active versus E. coli and B. cereus and contained only two bands, at 0.31 and 8.3 kDa, this last band probably responsible for its antimicrobial activity and chemically different from the other ones with a similar MW present in pools "c" and "e" that did not inhibit the growth of the pathogenic strains. This finding highlights the presence of bio molecules released during proteolytic hydrolysis that may contribute to the antimicrobial activity in donkey milk and play a significant role in the host defense system.
研究了驴奶中存在与溶菌酶不同的抑制病原菌生长的成分。将牛奶酸化,用胃蛋白酶水解,用高效液相色谱分析。在5个主要池中收集层析组分,去除溶菌酶后进行微电泳分析;对蜡样芽孢杆菌、金黄色葡萄球菌、粪肠球菌和大肠杆菌的抑菌活性进行了评价。水解乳的蛋白质含量在0.78 ~ 25.2 kDa之间。它对所有检测的致病菌株都有活性,抑制圈从4.3 mm(对大肠杆菌)到17.4 mm(对粪肠杆菌)不等。在HPLC步骤采集的池中,池“b”的蛋白质带最丰富,大部分分子量小于20.4 kDa,对除大肠杆菌外的所有病原菌都有最有效的抑制作用。池“a”对大肠杆菌和蜡样芽孢杆菌有活性,只有两个条带,分别为0.31和8.3 kDa,最后一个条带可能是其抗菌活性的原因,并且与池“c”和“e”中具有相似MW的其他条带在化学上不同,这些条带对致病菌株的生长没有抑制作用。这一发现强调了蛋白水解过程中释放的生物分子的存在,这些分子可能有助于驴奶的抗菌活性,并在宿主防御系统中发挥重要作用。
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引用次数: 37
Quality and safety assurance according to ISO 22000: 2005 in a meat delicatessen industry of Cyprus. 塞浦路斯肉类熟食行业的质量和安全保证符合ISO 22000: 2005。
Pub Date : 2010-06-22 DOI: 10.2174/1874256401004010030
A. Zorpas, C. Tzia, Irene Voukali, A. Panayiotou
Quality and safety are very important for the meat delicatessen industries. In Cyprus, food industries have started to apply safety programs the past few years in a preliminary way in order to comply with the current food safety directives or regulations. The verification activities in food industries encompass sampling for monitoring GMPs-GHPs and CCPs and determination of microbiological variables, review of records, flow diagrams and HACCP plan. The pre- sent paper describes the application of the above according to the standard ISO 22000:2005 in a meat delicatessen indus- try in Cyprus.
对于肉类熟食行业来说,质量和安全是非常重要的。在塞浦路斯,食品工业在过去几年中已经开始初步应用安全计划,以符合当前的食品安全指令或法规。食品工业的验证活动包括为监测gmp - ghps和ccp进行抽样,确定微生物变量,审查记录、流程图和HACCP计划。本文描述了上述根据标准ISO 22000:2005在塞浦路斯肉类熟食行业的应用。
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引用次数: 2
Antioxidative Activities of Palm Sugar-Like Flavouring 棕榈糖样香料的抗氧化活性
Pub Date : 2010-04-01 DOI: 10.2174/1874256401004010023
N. Amin, W. Mustapha, M. Y. Maskat, Ho Chun Wai
Maillard reaction products have been considered to have antioxidant capacities due to the reductones com- pound formations which contribute to antioxidant activity. Besides caramelisation, Maillard reaction takes place during the production of palm sugar. This paper reports the antioxidative activity of palm sugar-like flavourings and the commer- cial palm sugar (CPS) which was obtained from Pahang by using different types of methods. Formulation of palm sugar- like flavourings which had been optimised was obtained from the previous studies. Palm sugar-like flavourings (PSLFs) were prepared from sucrose and selected amino acids (Asparagine, Glutamine, Arginine and Lysine) at various ratios with buffer solutions (pH 7.86), heated at 143°C for 116 minutes. The results revealed that the PSLF (C) showed a significant difference (p<0.05) in reducing power, thiobarbituric acid (TBA) test, and radical scavenging activity (DPPH) compared to other formulations of PSLF and commercial palm sugar.
美拉德反应产物被认为具有抗氧化能力,因为还原化合物的形成有助于抗氧化活性。除了焦糖化作用外,棕榈糖生产过程中还发生美拉德反应。本文报道了棕榈类糖调味料的抗氧化活性,以及用不同方法从彭亨州提取的商品棕榈糖(CPS)的抗氧化活性。在前人的研究基础上,优化了棕榈糖类调味品的配方。以蔗糖和选定的氨基酸(天冬酰胺、谷氨酰胺、精氨酸和赖氨酸)按不同比例与缓冲液(pH 7.86)混合,在143℃下加热116分钟,制备棕榈糖样调味料(PSLFs)。结果表明,PSLF (C)在还原力、硫代巴比妥酸(TBA)测试和自由基清除活性(DPPH)方面与其他配方的PSLF和商业棕榈糖相比差异显著(p<0.05)。
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引用次数: 9
Food Applications and Physiological Effects of Anthocyanins as Functional Food Ingredients 花青素作为功能性食品成分在食品中的应用及生理效应
Pub Date : 2010-03-09 DOI: 10.2174/1874256401004010007
J. Shipp, E. Abdel‐Aal
Anthocyanins have been suggested as promising dietary compounds with an important role in human health. They are the largest group of water-soluble pigments in the plants, which are responsible for the red, purple and blue hues evident in fruits, vegetables, flowers and grains. As natural compounds of vegetables, fruits and red wines, anthocyanins are estimated to be widely consumed by humans with the estimated daily intake about 12.5 mg/d in the United States. In- terest in anthocyanin-rich foods and extracts has intensified recently because of their possible health benefits. Anthocyan- ins have been shown to be potent antioxidants as well as anti-diabetic, anti-carcinogenic and having ocular effects among others. Findings of their beneficial health effects support their role as natural food colorants, functional foods and dietary supplements. The present article summarizes our knowledge on the bioavailability, antioxidant activity and health- enhancing components of anthocyanin-rich foods and extracts. In addition, their current and potential use as a natural food colorant, functional food and dietary supplement in the food and beverage industry is discussed.
花青素已被认为是一种有前途的膳食化合物,对人体健康具有重要作用。它们是植物中最大的水溶性色素群,是水果、蔬菜、花卉和谷物中常见的红色、紫色和蓝色的原因。花青素作为蔬菜、水果和红酒中的天然化合物,据估计被人类广泛食用,在美国,估计每天的摄入量约为12.5毫克/天。由于富含花青素的食物和提取物可能对健康有益,最近人们对它们的兴趣日益浓厚。花青素已被证明是有效的抗氧化剂以及抗糖尿病、抗癌和具有眼部作用等。它们对健康有益的研究结果支持了它们作为天然食用色素、功能性食品和膳食补充剂的作用。本文综述了富含花青素的食品和提取物的生物利用度、抗氧化活性和保健成分的研究进展。此外,还讨论了它们作为天然食品着色剂、功能性食品和膳食补充剂在食品和饮料工业中的现状和潜在用途。
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引用次数: 167
Determination of moisture in powder and lyophilised saffron (Crocus sativus L.) by Karl Fischer method. 卡尔费希尔法测定藏红花粉和冻干藏红花中的水分。
Pub Date : 2010-02-01 DOI: 10.2174/1874256401004010001
D. Naviglio, S. Conti, L. Ferrara, A. Santini
Saffron is a spice obtained from the dried stigmas of the purple-flowered saffron crocus (Crocus sativus L.). The determination of the water content in saffron is very important in order to establish its microbiological stability and consequently to determine the commercial value. The average moisture content in dried saffron powder ranges between 10-12% causing a slow degradation of the product. On the other hand lyophilised saffron has lower water content and a better stability. The reference method commonly used for the determination of moisture in saffron it is based on the gra- vimetric loss of weight after heating at 105 °C for 16 hours. In this paper a procedure to obtain lyophilised saffron is proposed and two different methods, namely the infrared balance and the Karl Fischer titration, are used to determine moisture in dried and lyophilised saffron powder, and results are compared with the reference method. The Karl Fischer method, never applied so far to determine moisture in saffron, gave the best results. The average values determined were 6.87% and 4.01% (w/w) for conventionally dried and lyophi- lised saffron powder, respectively. The Karl Fischer titration method allows to determine in a faster manner the moisture in saffron powder and in lyophilised saffron, without any interference and using a minor quantity of saffron compared to the gravimetric procedure reported in the reference method. The volatile matter content, differently from the reference method, this way is not determined.
藏红花是一种香料,从开紫色花的藏红花(crocus sativus L.)的干柱头中提取。藏红花中水分含量的测定对确定其微生物稳定性和确定其商业价值具有重要意义。干藏红花粉的平均水分含量在10-12%之间,导致产品的缓慢降解。另一方面,冻干藏红花的含水量较低,稳定性较好。通常用于测定藏红花中水分的参考方法是在105°C加热16小时后的失重。本文提出了一种获得冻干藏红花粉的方法,并采用红外天平法和卡尔费歇尔滴定法测定了干燥和冻干藏红花粉中的水分,并与参考方法进行了比较。卡尔·费歇尔的方法给出了最好的结果,而这种方法迄今为止从未用于测定藏红花中的水分。常规干燥和冻干藏红花粉的平均含量分别为6.87%和4.01% (w/w)。卡尔菲舍尔滴定法允许以更快的方式确定藏红花粉和冻干藏红花中的水分,没有任何干扰,与参考方法中报告的重量法相比,使用少量藏红花。挥发物含量,与参考方法不同,此法不确定。
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引用次数: 12
Protective effect of thiols on wine aroma volatiles. 硫醇对葡萄酒香气挥发物的保护作用。
Pub Date : 2010-01-04 DOI: 10.2174/1874256400903010098
I. Roussis, D. Papadopoulou, M. Sakarellos-Daitsiotis
The ability of glutathione, oxidized glutathione, N-acetyl-cysteine, and N-acetyl-serine to protect wine aroma volatiles was examined. Muscat-white and Xinomavro-red wine were stored in open bottles at 20 o C and aroma volatiles were determined using solid phase microextraction along with GC-MS. Glutathione and N-acetyl-cysteine inhibited the decrease of several ace- tate esters, ethyl esters and terpenols, while oxidized glutathione and N-acetyl-serine did not. Present results show that the free -SH is responsible for the ability of glutathione and N-acetyl-cysteine to protect white and red wine aroma volatiles. Consequently, the protective action of any thiol in any wine may be taken into account.
考察了谷胱甘肽、氧化谷胱甘肽、n -乙酰半胱氨酸和n -乙酰丝氨酸对葡萄酒香气挥发物的保护作用。麝香白葡萄酒和xinomavro红葡萄酒20℃开瓶保存,采用固相微萃取-气相色谱-质谱法测定香气挥发物。谷胱甘肽和n -乙酰半胱氨酸抑制了几种ace-酸酯、乙酯和萜烯醇的减少,而氧化谷胱甘肽和n -乙酰丝氨酸则无此作用。目前的研究结果表明,游离sh对谷胱甘肽和n -乙酰半胱氨酸保护白葡萄酒和红葡萄酒香气挥发物的能力负责。因此,任何酒中任何硫醇的保护作用都可以考虑在内。
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引用次数: 27
Inhibition of the Oxidation of Corn Oil Stripped of Tocopherols and Refined Olive Oil by Thiols~!2009-05-05~!2009-06-21~!2009-12-24~! 硫醇对去除生育酚的玉米油和精制橄榄油氧化的抑制作用
Pub Date : 2010-01-04 DOI: 10.2174/1874256400903010103
I. Roussis, D. Papadopoulou, M. Sakarellos-Daitsiotis
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引用次数: 1
Inhibition of the Oxidation of Corn Oil Stripped of Tocopherols and Refined Olive Oil by Thiols 硫醇对去除生育酚的玉米油和精制橄榄油氧化的抑制作用
Pub Date : 2009-12-24 DOI: 10.2174/18742564009030100103
I. Roussis, D. Papadopoulou, M. Sakarellos-Daitsiotis
The ability of N-acetyl-cysteine and glutathione to inhibit the oxidation of corn oil stripped of tocopherols and refined olive oil was examined. The oxidative stability of corn oil stripped of tocopherols at 50 o C, 120 o C and 180 o C was evaluated. The absorbances at 234 nm and 270 nm, and p-anisidine value were monitored. N-acetyl-cysteine and glutathione inhibited the oxidation of oil, N-acetyl-cysteine at a higher degree. In comparison to BHA at 200 mg/L, both thiols at 20-40 mg/L exhibited lower (50 o C and 120 o C) or similar (180 o C) antioxidant activities. Compounds similar to the two thiols but not containing –SH group, N-acetyl-serine and oxidized glutathione, exhibited very low or no antioxidant activities. The oxidative stability of refined olive oil at 20 o C was determined by monitoring the peroxide value. N-acetyl-cysteine and glutathione at 20 mg/L exhibited antioxidant activities comparable to that of BHA at 200 mg/L. Present results show the ability of N-acetyl-cysteine and glutathione to inhibit the oxidation of corn oil stripped of tocopherols and refined olive oil indicating that thiols can inhibit the oxidation of any oil.
考察了n -乙酰半胱氨酸和谷胱甘肽对去除生育酚的玉米油和精制橄榄油氧化的抑制作用。考察了剥去生育酚的玉米油在50℃、120℃和180℃下的氧化稳定性。测定其在234 nm和270 nm处的吸光度及对茴香胺值。n -乙酰半胱氨酸和谷胱甘肽对油脂氧化的抑制作用较强。与200 mg/L的BHA相比,20-40 mg/L的两种硫醇具有较低(50℃和120℃)或相近(180℃)的抗氧化活性。与这两种硫醇相似但不含-SH基团、n -乙酰丝氨酸和氧化谷胱甘肽的化合物表现出极低或没有抗氧化活性。用过氧化值法测定了精制橄榄油在20℃时的氧化稳定性。20 mg/L的n -乙酰半胱氨酸和谷胱甘肽的抗氧化活性与200 mg/L的BHA相当。目前的研究结果表明,n -乙酰半胱氨酸和谷胱甘肽对去除生育酚的玉米油和谷胱甘肽的氧化有抑制作用,这表明硫醇可以抑制任何油的氧化。
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引用次数: 0
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The Open Food Science Journal
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